Use of fluorinated cyclic dinucleotides as oral vaccine adjuvants
10357560 ยท 2019-07-23
Assignee
Inventors
Cpc classification
A61K39/105
HUMAN NECESSITIES
A61K39/39
HUMAN NECESSITIES
A61K2039/6037
HUMAN NECESSITIES
A61K2039/57
HUMAN NECESSITIES
A61K2039/55561
HUMAN NECESSITIES
A61K31/7084
HUMAN NECESSITIES
International classification
A61K39/39
HUMAN NECESSITIES
A61K31/7084
HUMAN NECESSITIES
A61K39/09
HUMAN NECESSITIES
Abstract
Disclosed is the use of fluorinated cyclic dinucleotides and pharmaceutically acceptable salts thereof as an adjuvant for the preparation of an oral vaccine. Further disclosed is the use of fluorinated cyclic dinucleotides and pharmaceutically acceptable salts thereof for enhancing the immune response to an orally administered vaccine.
Claims
1. A method for preparing an oral vaccine, comprising admixing one or more non-replicating antigens with an adjuvant, wherein said adjuvant is a fluorinated cyclic dinucleotide having the following structure: ##STR00014## wherein A, C, A and C are independently selected from NH, O or S; X, Y, X, and Y are independently selected from O or S; Z and Z are independently selected from O, S, NH or CH.sub.2; and B.sub.1 and B.sub.2 are independently a purine selected from: ##STR00015## wherein Q is hydrogen or NH.sub.2; nitrogen is optionally substituted with C.sub.1-C.sub.6 alkyl or C.sub.1-C.sub.6 acyl group; and R is O or S.
2. The method according to claim 1, wherein the fluorinated cyclic dinucleotide is 2-F-c-di-GMP having the following structure: ##STR00016##
3. The method according to claim 1, wherein the oral vaccine is an oral vaccine for the prevention or treatment of a gastrointestinal infection and wherein the gastrointestinal bacterial infection is a H. pylori infection, a C. difficile infection or a Listeria infection.
4. An oral vaccine comprising a fluorinated cyclic dinucleotide or a pharmaceutically acceptable salt thereof and a composition comprising H. pylori antigen, wherein said fluorinated cyclic dinucleotide has the following structure: ##STR00017## wherein A, C, A and C are independently selected from NH, O or S; X, Y, X, and Y are independently selected from O or S; Z and Z are independently selected from O, S, NH or CH.sub.2; and B.sub.1 and B.sub.2 are independently a purine selected from: ##STR00018## wherein Q is hydrogen or NH.sub.2; nitrogen is optionally substituted with C.sub.1-C.sub.6 alkyl or C.sub.1-C.sub.6 acyl group; and R is O or S.
5. The oral vaccine according to claim 4, wherein the fluorinated cyclic dinucleotide is 2-F-c-di-GMP having the following structure: ##STR00019##
6. The oral vaccine according to claim 4, wherein the composition comprising H. pylori antigen comprises H. pylori cell free extract or one or more H. pylori specific peptides and/or proteins.
Description
BRIEF DESCRIPTION OF THE FIGURES
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DETAILED DESCRIPTION OF THE INVENTION
(7) Cyclic di-guanosine monophosphate, or 3,5-cyclic diguanylic acid, (c-di-GMP) has been shown to be a potent immunomodulator that stimulates cytokine and chemokines secretion and the maturation of dentritic cells (Chen et al. The potential of 3,5-cyclic diguanylic acid (c-di-GMP) as an effective vaccine adjuvant. Vaccine, 28, 3080-3085, 2010). An enhanced antigen-specific antibody response has been observed in mice administered interperitoneally with S. penuemoniae pneumolysin toxid (PdB) or pneumococal surface protection (PspA) adjuvanted with c-di-GMP (Chen et al, 2010; Ogunniyi et al. c-di-GMP is an effective immunomodulator and vaccine adjuvant against pneumococcal infection. Vaccine 26(36):4676-85, 2008). An enhanced antigen specific antibody response has also been observed in mice administered intranasally with pneumococcal surface adhesion A (Psa) together with c-di-GMP (Yan et al. 3,5-Cyclic diguanylic acid elicits mucosal immunity against bacterial infection. Biochemical and Biophysical Research Communications, 2009, 387(3):581-4). While the use of c-di-GMP as a potential mucosal adjuvant has been suggested (Chen et al., 2010), previous studies have not investigated the use of cyclic dinucleotides as adjuvants for orally administered vaccines. U.S. Pat. Nos. 7,592,326 and 7,569,555 suggest that c-di-GMP and the c-di-GMP analogues disclosed therein can be used as an adjuvant to modulate any vaccine. However, there is no disclosure of an orally administered vaccine adjuvanted with c-di-GMP or any analogue thereof. At best, there is only the suggestion that c-di-GMP can be combined with a vaccine which is administered intranasally, intraperitoneally, subcutaneously, intramuscularly or intradermally for treatment of a C. neoformans infection or as part of an influenza vaccine administered intramuscularly. None of the examples in U.S. Pat. Nos. 7,592,326 and 7,569,555 demonstrate or suggest the use of c-di-GMP or any other cyclic dinucleotide as an adjuvant for an orally administered vaccine. US Patent Application No. 2008/0286296 suggests that the cyclic dinucleotides and conjugates disclosed therein can be used as mucosal adjuvants. However, there is no disclosure of an orally administered vaccine adjuvanted with the disclosed cyclic dinucleotides and conjugates. The examples in US Patent Application No. 2008/0286296 only demonstrate or suggest the use of the c-di-GMP and c-di-AMP as an adjuvant for a vaccine administered intranasally or intraperitoneally.
(8) It is understood that orally administered vaccines as compared to intranasally, intraperitoneally, or other parenterally administered vaccines are subject to acid and enzymatic degradation in the gastrointenstinal (GI) tract and decreased availability due to first pass metabolism. In most cases, these vaccines are tolerated by the GI mucosal immune surfaces and thus do not lead to the induction of mucosal and systemic immune response. In addition, poor transport of antigens across the intestinal epithelium, antigen-microbiota interactions, and particle-mucous interactions in the GI lumen further impede the development of effective orally administered vaccines. Furthermore, mucosal adjuvants that are able to elicit immune response upon intranasal route of administration often fail to induce protective immunity when they are administered by oral route due to the compartmentalization of the mucosal immune system. Consequently, only a small fraction of mucosal adjuvants function when administered orally, although they work well when administered intranasally. As discussed above, currently available adjuvants and delivery systems for orally administered vaccines have unacceptable toxicities or poor adjuvanticity. The present disclosure is the first to describe the use of fluorinated cyclic dinucleotides, such as 2-F-c-di-GMP, as adjuvants for orally administered vaccines for enhancing immunogenicity of orally administered vaccines.
(9) As used herein, antigen means a compound or composition which, when introduced into an animal or a human in the appropriate context, will result in the formation of antibodies and/or cell-mediated immunity. Non-replicating antigens include but are not limited to modified inactivated infectious agents such as viruses and microorganisms (including bacteria, fungi, and yeast) or parts of such infection agents (including peptides, proteins, nucleic acids, oligosaccharides and polysaccharides).
(10) As used herein, adjuvant means a compound or compounds that, when used in combination with appropriate antigens, augment, or otherwise alter or modify the resultant immune responses.
(11) As used herein, vaccine means a composition comprising one or more antigens that is administered, typically with an adjuvant, to an animal or human to produce an antigen-specific immune response.
(12) As used herein, oral vaccine means a vaccine which is orally administered to an animal or human.
(13) As used herein, enhanced immune response means the increased magnitude or quantities of the reaction of the cells and fluids of the body to the presence of a substance that is not recognized as a constituent of the body itself. The immune response can be an humoral response, which involves the transformation of B cells into plasma cells that produce and secrete antibodies to a specific antigen, and/or a cell-mediated response produced when sensitized T cells directly attack foreign antigens and secrete lymphokines that initiate the body's humoral immune response.
(14) Disclosed herein is the use of a fluorinated cyclic dinucleotide according to Formula (I):
(15) ##STR00010##
wherein A, C, A and C are independently selected from NH, O or S; X, Y, X, and Y are independently selected from O or S; Z and Z are independently selected from O, S, NH or CH.sub.2; and B.sub.1 and B.sub.2 are independently a purine selected from:
(16) ##STR00011##
wherein Q is hydrogen or NH.sub.2; nitrogen is optionally substituted with C.sub.1-C.sub.6 alkyl or C.sub.1-C.sub.6 acyl group; and R is O or S,
or a pharmaceutically acceptable salt thereof, as an adjuvant for the preparation of an oral vaccine or for the enhancement of an immune response in response to an orally administered vaccine.
(17) In an embodiment, the fluorinated cyclic dinucleotide according to Formula (I) is 2-F-c-di-GMP having the following structure.
(18) ##STR00012##
(19) The fluorinated cyclic dinucleotide according to Formula (I) can be formulated as neutral or salt forms. Pharmaceutically acceptable salts include those formed with cations such as those derived from but not limited to sodium, potassium, ammonium, calcium, ferric hydroxides, isopropylamine, triethylamine, 2-ethylamino ethanol, histidine, or procaine.
(20) The fluorinated cyclic dinucleotide according to Formula (I) is expected to have good stability in the gastrointestinal tract and particularly, resistance to acid degradation in stomach fluids. Without being bound to any particular theory, it is believed that resistance to degradation in the gastrointestinal tract enhances the efficacy of the fluorinated cyclic dinucleotide according to Formula (I) to act as an oral adjuvant and to enhance the immune response to oral vaccines. A further benefit of the fluorinated cyclic dinucleotide according to Formula (I) is that it can be easily synthesized in comparison to many other cyclic dinucleotides. For example, 2-F-c-di-GMP is easier to synthesize as compared with c-di-GMP as the synthesis of 2-F-c-di-GMP does not require protection on the 2-OH as is the case for c-di-GMP.
(21) The fluorinated cyclic dinucleotide according to Formula (I) or a pharmaceutically acceptable salt of thereof, can be used as an adjuvant for the preparation of an oral vaccine. The oral vaccine is preferably a vaccine which produces an antigen-specific immune response at local (site of immunization) and distal mucosal tissues (such as the respiratory and urogenital systems) as well as in the systemic tissues (such as blood, etc.) when administered orally. In one embodiment, the oral vaccine is for the prevention or treatment of an infection caused by bacteria, viruses, parasites, protozoa, or fungus. In a further embodiment, the oral vaccine is for the prevention or treatment of a gastrointestinal infection. Examples of gastrointestinal infections include infections caused by H. pylori, E. coli, C. jejuni, S. typhimurium, rotavirus, norovirus, C. difficile and Listeria. Such oral vaccines can comprise one or more non-replicating antigens. The one or more non-replicating antigens may include native, modified, or inactivated whole cells, and cell components, including peptides, proteins, nucleic acids, oligosaccharides and polysaccharides.
(22) In one embodiment, the oral vaccine is an oral vaccine for the prevention or treatment of a H. pylori infection and a fluorinated cyclic dinucleotide according to Formula (I) or a pharmaceutically acceptable salt is used as an adjuvant. In a preferred embodiment the fluorinated cyclic dinucleotide according to Formula (I) is 2-F-c-di-GMP. The oral vaccine can comprise H. pylori cell free extracts or one or more H. pylori specific peptides and/or proteins.
(23) Disclosed is a method for preparing an oral vaccine comprising admixing one or more non-replicating antigens with an adjuvant comprising the fluorinated cyclic dinucleotide according to Formula (I) and more preferably, 2-F-c-di-GMP. The oral vaccine can be prepared as a single oral dosage form wherein the fluorinated cyclic dinucleotide according to Formula (I) or pharmaceutically acceptable salt thereof are admixed with the one or more non-replicating antigens.
(24) The relative effective amounts of the components comprising the oral vaccine, including the one or more non-replicating antigens and the fluorinated cyclic dinucleotide or pharmaceutically acceptable salt thereof, can be determined using methods known in the art. Typically, this involves testing vaccine formulations in animals starting with a low dose of the formulation and then increasing the dosage while monitoring the specific immune response and the protection against infection.
(25) For embodiments wherein the oral vaccine is an oral vaccine for the prevention or treatment of H. pylori infection and a fluorinated cyclic dinucleotide according to Formula (I), preferably 2-F-c-di-GMP, or a pharmaceutically acceptable thereof, is used as an adjuvant, the weight ratio of H. pylori cell free extract (HPCE) to the fluorinated cyclic dinucleotide according to Formula (I) or pharmaceutically acceptable salt thereof can be from about 1 to 50, more preferably about 1 to 10, and even more preferably about 1 to 5.
(26) In addition to the antigen and adjuvant compounds, the oral vaccine may comprise suitable carriers, excipients and auxiliaries which facilitate the preparation of dosage forms for oral administration, including solutions or other liquid forms, tablets, capsules, and granules. The oral dosage forms may be provided with a suitable enteric coating, if desired.
(27) The oral dosage forms may include excipients including fillers, binders, and disintegrating agents. Examples of fillers include sugars (e.g. lactose or sucrose mannitol or sorbitol), cellulose preparations, and/or calcium phosphates (e.g. tricalcium phosphate or calcium hydrogen phosphate). Examples of binders include starches (e.g. maize starch, wheat starch, rice starch, or potato starch), gelatin, gum tragacanth, methyl cellulose, hydroxypropylmethylcellulose, sodium carboxymethylcellulose, and/or polyvinyl pyrrolidone. Examples of disintegrating agents include starches discussed above, as well as carboxymethyl starch, cross-linked polyvinyl pyrrolidone, agar, or alginic acid or a salt thereof. The oral dosage forms may further include auxiliaries including flow-regulating agents and lubricants, such as silica, talc, stearic acid or salts thereof (e.g magnesium stearate or calcium stearate) and/or polyethylene glycol.
(28) The oral vaccines disclosed herein can be manufactured by methods known in the art including but not limited to mixing, granulating, dissolving, lyophilizing processes. It will be understood that the choice of manufacturing process will depend on the physical properties of the vaccine ingredients used.
(29) Many bacterial (for example, Campylobacter and E. coli) and viral (for example, rotavirus and norovirus) pathogens are extracellular organisms or have a significant extracellular life for their colonization, replication, and dissemination. It is well known and demonstrated in the published literature that antigen-specific IgA at mucosal sites and IgG in the blood are protective against the infections caused by these pathogens. It has now been determined that the fluorinated cyclic dinucleotide according to Formula (I) can be used to enhance the immune response elicited by oral vaccines for such pathogens.
(30) In another aspect, provided is a method for enhancing an immune response to an orally administered vaccine, comprising: administering an oral vaccine with an effective amount of an adjuvant comprising of a fluorinated cyclic dinucleotide according to Formula (I) or a pharmaceutically acceptable salt thereof.
(31) In an embodiment, the fluorinated cyclic dinucleotide is 2-F-c-di-GMP having the structure shown below.
(32) ##STR00013##
(33) The fluorinated cyclic dinucleotide according to Formula (I) or pharmaceutically acceptable salt thereof can be used to enhance the immune response to an oral vaccine which preferably produces an antigen-specific mucosal immune response when administered. In one embodiment, the oral vaccine is for the prevention or treatment of an infection caused by bacteria, viruses, parasites/protozoa, and fungus. In a further embodiment, the oral vaccine is for the prevention or treatment of an infection. Examples of gastrointestinal infections include infections caused by H. pylori, E. coli, C. jejuni, S. typhimurium, rotavirus, norovirus, C. difficile and Listeria. Such oral vaccines can comprise one or more non-replicating antigens. The one or more non-replicating antigens may include inactivated whole cells, and cell components, including peptides, proteins, nucleic acids, oligosaccharides and polysaccharides.
(34) In one embodiment, the oral vaccine is an oral vaccine for the prevention or treatment of a H. pylori infection and a fluorinated cyclic dinucleotide according to Formula (I), preferably 2-F-c-di-GMP, or a pharmaceutically acceptable salt thereof is used as an adjuvant. The oral vaccine can comprise H. pylori extracts cell free extracts or one or more H. pylori specific peptides or proteins.
(35) Administration of the oral vaccine with an effective amount of an adjuvant comprising a fluorinated cyclic dinucleotide according to Formula (I) or a pharmaceutically acceptable salt thereof enhances the immune response elicited by the oral vaccine alone. Enhancement of the immune response may include an antigen-specific mucosal immune response which would otherwise be absent or elicited to a lesser degree when an equal dosage of a selected oral vaccine is administered to a host without the adjuvant (for example, as evidenced by lowered antibodies titres, cell proliferation or secretion of certain cytokines or toxic substances, or expression of activation markers in the surface of immune cells). The enhanced mucosal immune response may be characterized by an antigen-specific secretive IgA antibody response at mucosal sites. The enhanced immune response may further comprise an antigen-specific humoral immune response and/or an antigen-specific cellular immune response either of which would otherwise be absent or elicited to a lesser degree when an equal dosage of the selected oral vaccine is administered to a patient without the adjuvant.
(36) It will be understood that the type of immune response and the degree of enhanced immunogenicity will depend on the type and amount of oral vaccine administered and the recipient. The relative effective amounts of the oral vaccine and adjuvant can be determined using conventional methods as described above.
(37) For embodiments wherein the oral vaccine is an oral vaccine for the prevention or treatment of H. pylori infection and a fluorinated cyclic dinucleotide according to Formula (I), preferably 2-F-c-di-GMP, or a pharmaceutically acceptable thereof is used as an adjuvant, the weight ratio of H. pylori cell free extract (HPCE) to the fluorinated cyclic dinucleotide according to Formula (I) or pharmaceutically acceptable salt thereof can be from about 1 to 50, more preferably about 1 to 10, and even more preferably about 1 to 5.
(38) In one embodiment, the vaccine and the adjuvant can be orally administered as separate dosage forms.
(39) In another embodiment, the vaccine and the adjuvant can be orally administered as an admixture in a unitary dosage form, including as any of the oral dosage forms described above.
(40) While this invention has been described in connection with specific embodiments and chemical structures, it will be apparent to those skilled in the art that variations and modifications may be made without departing from the invention. The invention includes all such variations and modifications as fall within the scope of the appended claims. The following examples further illustrate the invention and are not intended to limit the invention in any manner.
EXAMPLES
(41) Materials and Methods
(42) c-di-GMP. c-di-GMP was synthesized according to procedures reported previously. (Yan, H.; Anguilar, A. L. Synthesis of 3,5-cyclic diguanylic acid (c-di-GMP), Nucleoside, Nucleotides & Nucleic Acids. 2007, 26, 189.)
(43) 2-F-c-di-GMP. 2-F-c-di-GMP was synthesized according to procedures reported previously (Yan, H.; Anguilar, A. L. Synthesis of 3,5-cyclic diguanylic acid (c-di-GMP), Nucleoside, Nucleotides & Nucleic Acids. 2007, 26, 189).
(44) Cholera toxin (CT). CT was purchased from Sigma-Aldrich Canada Ltd. (Oakville, Ontario, Canada).
(45) Production of recombinant pneumococcal surface adhesion A (PsaA) of S. pneumonia. The PsaA gene from type 14 isolate of S. pneumoniae was amplified by colony PCR using the primer set PsaA-BspM-F (GAAGAAACCTGCACAACAACAGGCCGCTAGCGGAAAAAAAGATRCARCTTC) and PsaAH-Hind-R (TAATAAAAGCTTTCACTAATGGTGATGGTGATGGTGGGATCCACCGCCTCCT TTTGCCAATCCTTCAGCAATC). The reaction also in-frame fused DNA encoding 6 His tag to the 3-end of the PsaA gene. The PCR product was digested with BspMI and HindIII and ligated into a periplasmic expression vector pSJF2 (Tanha J, Muruganandam A, Stanimirovic D (2003). Phage display technology for identifying specific antigens on brain endothelial cells. Methods Mol. Med. 89: 435-449), which was further digested with the same enzymes to generate a clone expressing PsaAH. The PsaAH clone was then purified with immobilized metal affinity chromatography (IMAC) as previously described (Zhang J, Tanha J, Hirama T, Khieu N H, To R, et al. (2004). Pentamerization of single-domain antibodies from phage libraries: a novel strategy for the rapid generation of high-avidity antibody reagents. J. Mol. Biol. 335: 49-56.). The recombinant Escherichia coli culture harboring the plasmid encoding for PsaAH was cultivated at 28 C. in 24 L of Luria broth supplemented with 0.2 mg/ml penicillin, in a fermenter vessel. The starting culture optical density (OD.sub.600) was 0.17, and the pH was maintained at 7.2 and the dissolved oxygen at 20% saturation during cultivation. Once an OD.sub.660 of 0.50 was achieved, 2 g isopropyl-beta-D-thiogalactopyranoside (IPTG) was added to induce the culture. At 21 h post-induction, the culture had reached an OD.sub.600 of 3.90, and approximately 180 g wet wt of cells were collected upon harvesting by a combination of ultrafiltration and centrifugation. The cells were frozen at 20 C. until used for PsaA extraction. Cell pellets were lysed with an Emulsiflex Cell Disruptor (Avestin Inc. Ottawa, ON). Following centrifugation of the cell lysate, the clear supernatant was loaded onto a Hi-Trap Chelating affinity column (Amersham Biosciences) and the 6 His-tagged proteins were purified following the manufacturer's instructions. A total of 47 mg of PsaA protein was obtained.
(46) Preparation of H. pylori cell-free sonicate extract (HPCE). H. pylori SS1 were grown on Columbia agar supplemented with 10% Laked horse blood for 48-72 h at 37 C. under microanaerobic conditions generated with CampyGen in an AnaeroJar (Oxoid, Hampshire, UK). The bacterial cells were harvested by centrifugation at 12 000 g for 10 min at 4 C., washed with phosphate-buffered saline (PBS) three times and then suspended in 15 mL PBS. The cells were disrupted by sonication with a Branson sonifier (model B15, Branson Sonic Power Company, Banbury, Conn., USA) at 30 s intervals (60% pulses) for 5 min on ice. The cell debris and unbroken cells were removed by centrifugation at 14,000 g for 20 min at 4 C. The supernatant was aliquoted and stored at 80 C. until use. Protein concentrations of the extract were determined by the Bradford method (Bio-Rad Laboratories, Hercules, Calif., USA) and by using bovine serum albumin as the standard.
(47) Mice. Six to eight-week-old female BALB/c or C57BL/6 mice were purchased from Charles Rivers Laboratories (St. Constant, Quebec). The animals were housed under specific-pathogen-free conditions in a federally licensed small animal containment level 2 facility, and given free access to sterile water and certified mouse chow. The animals were maintained and used in accordance with the recommendations of the Canadian Council on Animal Care Guide to the Care and Use of Experimental Animals, and the experimental procedures were approved by the institutional animal care committee.
(48) Intranasal or oral immunization in mice. For intranasal immunization, mice were lightly anesthetized under isofluorane. On day 0, 14 and 21, groups of mice (n=5) were inoculated with 50 l of various vaccine preparations or controls as detailed in Examples 1-5. For oral immunization, various vaccine preparations or controls in 0.2 ml volume were administered by gavage via an 18-gauge feeding needle.
(49) At day 28, blood and mucosal samples (fecal pellets or/and vaginal wash) were collected as described elsewhere (Patel G B, Zhou H, Ponce A, Chen W (2007). Mucosal and systemic immune responses by intranasal immunization using archaeal lipid-adjuvanted vaccines. Vaccine 25: 8622-8636.). In some experiments, nasal wash samples were also collected at the end of experiment. For both the vaginal and nasal wash, the volume of the lavage fluid recovered from each mouse was recorded. Since the recovery of lavage volumes among different mice in our study were very similar (about 90%), the small variation among individual samples was not adjusted. All samples were stored at 20 C. until assay.
(50) Vaginal wash samples were collected by slowly injecting and withdrawing (3-4 times) 50 l PBS (pH 7.2) into the vagina of conscious mice, using a P100 pipette. The volume of the vaginal lavage fluid recovered from each mouse was recorded.
(51) Enzyme-linked immunosorbent assay (ELISA) for PsaA-specific IgA and IgG isotypes. Levels of PsaA-specific antibodies in serum and mucosal samples were measured by an enzyme-linked immunosorbent assay (ELISA) modified from a previously described procedure (Yan H, Kuo Lee R, Tram K, Qiu H, Zhang J, Patel G B, Chen W. 3,5-Cyclic diguanylic acid elicits mucosal immunity against bacterial infection. Biochemical and Biophysical Research Communications, 2009; 387(3):581-4.). Briefly, 96-well Immunolon 2.sup.R microplates (Thermo Electron Corporation, Milford, Mass.) were coated with 0.5 g PsaA/well in 100 l of 0.015 M sodium carbonate and 0.035 M sodium bicarbonate buffer (pH 9.6) at 4 C. overnight. All the subsequent incubations were carried out at room temperature. The wells were blocked by incubation with 5% bovine serum in PBS for 1 h, and then rinsed three times with PBS-0.05% Tween 20. Duplicates of 100 l pre-diluted samples were added to the wells. Sample dilutions were as follows: 1:2 for fecal extracts and nasal washes, 1:2000 for serum IgG1, 1:500 for serum IgG2a and 1:50 for serum IgA. After the plates were incubated for 3 h, alkaline phosphatase-conjugated goat antibodies specific for mouse IgA, IgG1 and IgG2a (all from Caltag Laboratories, Burlingame, Calif.) were added and incubated for 1 h. Color reactions were developed by the addition of p-nitrophenyl phosphate (pNPP) substrate (KPL, Inc., Gaithersburg, Md.), and optical density was measured at 405 nm with an automated ELISA plate reader (Multiskan Ascent, Thermo Labsystems, Vantaa, Finland). Pooled samples collected from mice that had been intranasally immunized with the PsaA+CT or from the nave mice were used as positive or negative controls respectively.
(52) Measurement of H. pylori-specific antibodies in the serum and mucosal samples. Antibodies specific for H. pylori were measured by an enzyme-linked immunosorbent assay (ELISA) modified from a previously described procedure (Chen W, Shu D, Chadwick V S. Reduced colonization of gastric mucosa by Helicobacter pylori in mice deficient in interleukin-10. J. Gastroenterol. Hepatol. 2001 April; 16(4):377-83.). Briefly, 96-well Immunolon 4 plates (Dynex Technologies Inc., Chantilly, Va., USA) were coated with sonicated whole-cell extracts of H. pylori SS1 (20 g/mL; 50 L/well) in carbonate-bicarbonate buffer (pH 9.5) at 4 C. overnight. The plates were blocked by an incubation with 1% gelatin in phosphate-buffered saline at room temperature for 1 h, and then rinsed four times with PBS with 0.05% Tween 20. Prediluted 100 L serum samples (1:50) were added to the wells, and the plates were incubated at room temperature for 3 h. Thereafter, alkaline phosphatase-conjugated goat antibodies specific for mouse IgA, and IgG (Sigma Chemical Co., St. Louis, Mo., USA), and horseradish peroxidase-conjugated rabbit antibodies specific for mouse IgG1, IgG2a, IgG2b, IgG3 and IgM (all from Serotec Ltd, Oxford, UK) were added for 2 h at room temperature. Color reactions were developed by the addition of p-nitrophenyl phosphate (pNPP) or 2,2-azinobis(3-ethylbenzthiazolinesulfonic acid) (Sigma) substrates, and optical density was measured at 405 nm with an automated ELISA plate reader (Anthos 2001; Anthos Labtec, Salzburg, Austria). Controls for the assay included serum samples from mice orally immunized with H. pylori antigens with cholera toxin as an adjuvant, and serum samples from nave mice, respectively.
(53) Intranasal challenge of mice with S. pneumoniae. Fresh inocula were prepared for each experiment from a frozen stock of S. pneumoniae (type 14). Stock vials of S. pneumoniae were thawed and the culture revived on chocolate agar, which was then used to inoculate Todd-Hewitt and Columbia broth supplemented with 1% glucose and 0.1% sodium bicarbonate. The broth culture was incubated in a candle jar at 37 C. for approximately 6 h. The broth culture in mid-logarithmic growth phase was centrifuged at 12,000g for 15 min, cells were resuspended in PBS and used immediately. Fourteen days after the last immunization, mice were anesthetized and intranasally inoculated with approximately 10.sup.7 colony-forming units (CFU) S. pneumoniae in 50 l saline. The actual inoculum concentrations were determined by plating 10-fold serial dilutions on chocolate agar. Inoculated mice were sacrificed 3 days later and the nasal cavity was lavaged with 0.5 ml lavage fluid and aliquots (100 l) of 10-fold serial dilutions of the lavage fluids were cultured, in duplicates, on chocolate agar plates to quantify the number of viable organisms.
(54) H. pylori and oral H. pylori infection. The mouse-adapted H. pylori SS1 that was established by Lee et al. (Lee A, O'Rourke J, De Ungria M C, Robertson B, Daskalopoulos G, Dixon M F. A standardized mouse model of Helicobacter pylori infection. Gastroenterology 1997; 112: 1386-97.) was used for all experiments. Bacteria were grown on brain-heart infusion (BHI) broth supplemented with 5% horse serum under a microaerophilic atmosphere created by a CampyGen generator (Oxoid Ltd, Hampshire, England) at 37 C. for 48 h. The organisms were positively identified on the basis of morphology and presence of urease enzyme activity, and via PCR amplification of the cagA gene by using specific primers.
(55) The mice were inoculated orally with 110.sup.8 colony-forming units (CFU) of freshly harvested H. pylori in 500 L BHI broth by using a 19-gauge feeding needle as described previously. The inoculations were repeated twice (a total of three inoculations) over a period of 5 days. Mice were killed by an overdose of carbon dioxide 4-6 weeks after the last inoculation, and the serum and stomachs of the animals were collected for analysis.
(56) Assessment of H. pylori infection. The presence of H. pylori infection in individual mice was determined by quantitative bacterial culture as described previously (Chen W, Shu D, Chadwick V S. Reduced colonization of gastric mucosa by Helicobacter pylori in mice deficient in interleukin-10. J. Gastroenterol. Hepatol. 2001 April; 16(4):377-83.). Briefly, the mouse stomach was opened longitudinally along the greater curvature and gently washed three times in PBS to remove the stomach contents. The stomach tissue was then homogenized in 5 ml saline with Teflon pestles and glass tubes. The homogenates were serially diluted in saline, and 100 l of the dilutions were plated onto GSS (Glaxo Selective Supplement Antibiotics)-supplemented agar plates. The plates were incubated for 96 h at 37 C. under microaerophilic conditions, and the number of colonies were counted and expressed as CFU/stomach. With this method, log.sub.in 2.7 CFU/stomach represented the limit of detection. The data were analyzed using the Mann-Whitney test, using GraphPad software version 5.0.
(57) Preparation of flagellins from L. monocytogenes and C. difficile. Bacterial strains were grown overnight at 37 C. on agar plates. Cells were scraped from surface and resuspended in 10 mM Tris pH7.4. Flagella were sheared from surface of bacterial cells using a warring blender (41 min). Bacterial cells were removed by low speed centrifugation and then flagellar filaments were collected from supernatant by ultracentrifugation (100,000g, 1 hour). Flagellar samples were analysed by SDS-PAGE.
(58) Measurement of flagellin-specific antibodies in the serum and mucosal samples. Antibodies specific for L. monocytogenes flagellin and C. difficile flagellin were measured by an enzyme-linked immunosorbent assay (ELISA) modified from a previously described procedure (Chen W, Shu D, Chadwick V S. Reduced colonization of gastric mucosa by Helicobacter pylori in mice deficient in interleukin-10. J. Gastroenterol. Hepatol. 2001 April; 16(4):377-83.). Briefly, 96-well Nunc Immunoplate II plates (VWR) were coated with purified flagellins (5 g/mL; 50 L/well) in carbonate-bicarbonate buffer (pH 9.5) at 4 C. overnight. The plates were blocked by an incubation with 1% bovine serum albumin in phosphate-buffered saline with 0.1% NaN.sub.3 at room temperature for 1 h, and then rinsed three times with PBS with 0.05% Tween 20. Prediluted 100 L serum samples (1:100) or fecal extracts (1:2 dilution) were added to the wells, and the plates were incubated at room temperature for 3 h. Thereafter, alkaline phosphatase-conjugated rabbit antibodies specific for mouse IgA, IgG1, and IgG2a (all from Serotec Ltd, Oxford, UK) were added for 1 h at room temperature. Color reactions were developed by the addition of p-nitrophenyl phosphate (pNPP)(Sigma) substrates, and optical density was measured at 405 nm with an automated ELISA plate reader (Anthos 2001; Anthos Labtec, Salzburg, Austria).
(59) Statistical analysis. Data are presented as meanSD for parametric data, and median with ranges for non-parametric data. Differences in the number of bacteria between groups of animals were determined by using the Student's t-test. Differences in the severity of gastritis and antibody titers were determined by using the Mann-Whitney
(60) U-test. Differences were considered significant for a P value of 0.05.
Example 1Intranasally Administered 2-F-c-di-GMP Induces Strong Antigen-Specific Antibody Responses in the Serum and at Multiple Mucosal Sites
(61) To determine if 2-F-c-di-GMP can elicit mucosal immune responses to microbial (bacterial and viral) antigens, groups of BALB/c mice were intranasally (i.n.) immunized with 2 g recombinant PsaA protein of S. pneumoniae admixed with 2.5 g 2-F-c-di-GMP, 2.5 g c-di-GMP, or 10 g c-di-GMP at day 0, 14 and 21. An additional group of mice was sham-immunized with PBS only. The blood and mucosal samples (fecal pellets and vaginal wash) were collected at day 28 and assayed for PsaA-specific IgA, IgG1 and IgG2a by standard ELISA. As seen in
Example 2Intranasal Immunization of Mice with 2-F-c-di-GMP-Adjuvanted PsaA Reduces Nasopharyngeal Colonization by S. Pneumoniae
(62) Next, it was determined if the mucosal immune responses induced by the 2-F-c-di-GMP adjuvanted vaccine are protective against mucosal infections in the well-established mouse S. pneumoniae colonization model. Groups of immunized (PsaA+2-F-c-di-GMP) or sham-immunized (saline only) mice were intranasally challenged with 4.5510.sup.6 CFU S. pneumoniae at day 35 after the first immunization. Mice were sacrificed 3 days after challenge, and the nasopharyngeal tract was lavaged and bacterial numbers in the nasal wash were determined. As seen in
Example 3Oral Immunization with 2-F-cdiGMP-Adjuvanted Vaccine Induces Strong Antigen-Specific Antibody Responses in the Serum and at Multiple Mucosal Sites
(63) To determine if oral administration of 2-F-c-di-GMP can induce mucosal immune responses to microbial (bacterial and viral) antigens, groups of C57BL/6 mice (n=5) were orally immunized with varying amounts (125-500 g) of HPCE admixed with 100 g 2-F-c-di-GMP, or 500 g HPCE admixed with 10 g CT at day 0, 14 and 21. Additional group of mice was sham-immunized with PBS only. The blood and fecal pellets were collected at day 28 and assayed for HPCE-specific IgA, IgG1 and IgG2a by standard ELISA. As it can been seen in
Example 4Oral Immunization with 2-F-c-di-GMP-Adjuvanted Vaccine Induces Antigen-Specific Th1/Th17 Cytokine Responses
(64) To determine if oral administration of 2-F-c-di-GMP can induce antigen-specific Th1/Th17 cytokine responses to microbial (bacterial and viral) antigens, Groups of 5 C56BL/6 mice were orally immunized with varying amount of H. pylori cell free sonicate extract (HPCE) admixed with either 100 g 2-F-c-di-GMP or 10 g cholera toxin (CT, positive control) at day 0, 14 and 21. Additional group of mice were immunized with PBS and served as sham-immunized group. The spleen were collected at day 28 and single cell suspension was prepared for the determination of antigen-specific cytokine responses. The cells were stimulated by 10 g/ml HPCE and cultured at 37 C. in a 5% CO.sub.2 atmosphere for 72 hours. At the end of the culture, the supernatant was collected and assayed for the levels of IFN-, IL-2 and IL-17 by Luminex. The production of antigen-specific IFN- and IL-17 is associated with the protection against H. pylori infection. As it can be seen in
Example 5Oral Immunization of Mice with 2-F-c-di-GMP-Adjuvanted HPCE Significantly Reduces Gastric Colonization by H. Pylori
(65) Next, it was determined if the mucosal immune responses induced by the 2-F-c-di-GMP adjuvanted H. pylori vaccine are protective against mucosal infections in the well-established mouse model of H. pylori (Lee A, O'Rourke J, De Ungria M C, Robertson B, Daskalopoulos G, Dixon M F. A standardized mouse model of Helicobacter pylori infection. Gastroenterology 1997; 112: 1386-97). Groups of immunized (HPCE+2-F-c-di-GMP or HPCE+CT) or sham-immunized (PBS only) mice were orally challenged with 10.sup.8 CFU H. pylori SS1 three times at every second day, starting at day 35 after the first immunization. Mice were sacrificed 6 weeks after the first challenge, and the stomach tissue was collected and processed for the determination of the bacterial burdens. As seen in
Example 6Oral Immunization with 2-F-cdiGMP-Adjuvanted Vaccine Induces Strong Antigen-Specific Antibody Responses in the Serum and at Multiple Mucosal Sites
(66) As an additional example to demonstrate the capability of oral administration of 2-F-c-di-GMP can induce mucosal immune responses to microbial (bacterial and viral) antigens, groups of BALB/c mice (n=3) were orally immunized with different amounts of bacterial flagellin antigens purified from Listeria (50 g) or C. difficile (30 g) admixed with 75 g 2-F-c-di-GMP at day 0, 14 and 21. Additional group of mice was sham-immunized with PBS only. The blood and fecal pellets were collected at day 35 and assayed for flagellin-specific IgA, IgG1 and IgG2a by standard ELISA. As it can been seen in