A SYNERGISTIC PLANT GROWTH STIMULANT COMPOSITION COMPRISING MELATONIN AND NITROGENOUS COMPOUNDS FOR IMPROVING PLANT GROWTH AND DECREASING BIOTIC-ABIOTIC STRESSES ON PLANTS

Abstract

The invention disclosed herein is a synergistic plant growth stimulant composition comprising Melatonin and Nitrogenous compounds for improving plant growth, enhancing yield and decreasing the biotic-abiotic stresses on plants. The invention also disclosed herein is a process for preparation of said synergistic composition.

Claims

1. A synergistic plant growth stimulant composition for improving plant growth, enhancing yield and decreasing biotic-abiotic stresses on plants comprising: a) Melatonin; b) Nitrogenous compounds selected from urea and derivatives thereof amino acids and derivatives thereof proteins, protein digest and amines, either alone or in any combination thereof; and c) adjuvants and/or surfactant and/or excipients; wherein the ratio of Melatonin and Nitrogenous compounds varies from 0.5:1, 1:1, 1:10 and 1:20.

2. The synergistic plant growth stimulant composition as claimed in claim 1, wherein the Melatonin is present in an amount ranging from 0.01% to 10% and the Nitrogenous compounds are present in an amount ranging from 1 to 50% of the total composition.

3. The synergistic plant growth stimulant composition as claimed in claim 1, wherein the urea and its derivatives are selected from N, N-diformyl urea, N, N-diacetyl urea, di-substituted diaryl ureas, dialkyl ureas, diacyl ureas, aryl ureas, alkyl ureas, urea formaldehyde (including oligomers and polymers) and urea acrylamide compounds either alone or in combination thereof.

4. The synergistic plant growth stimulant composition as claimed in claim 1, wherein the amino acids and derivatives thereof are selected from alanine, valine, methionine, leucine, iso-leucine and phenylalanine, either alone or in any combination thereof.

5. The synergistic plant growth stimulant composition as claimed in claim 1, wherein the proteins or protein digest are selected from protein hydrolysate and/or peptide combination and/or enzymes, either alone or in my combination thereof.

6. The synergistic plant growth stimulant composition as claimed in claim 1, wherein the amines are selected from amidates, imidates, amine salts, amine oxides, polyamines, amines azides and aziridines, either alone or in any combination thereof.

7. The synergistic plant growth stimulant composition as claimed in claim 1, wherein the adjuvants and/or surfactant and/or excipients are selected from anionic, non-ionic and cationic surfactants, carboxy methyl cellulose (CMC), and lignosulfonates.

8. The synergistic plant growth stimulant composition as claimed in claim 7, wherein the adjuvants and/or surfactant and/or excipients are present in an amount of 0.1 to 5% of the total composition.

9. The synergistic plant growth stimulant composition as claimed in claim 1, wherein the ratio of said composition and adjuvants/surfactant/excipients is about 1:0.25.

10. The synergistic plant growth stimulant composition as claimed in claim 1, wherein the composition further comprises plant growth hormones selected from Gibberelic acid and abscisic acid.

11. The synergistic plant growth stimulant composition as claimed in claim 10, wherein the plant growth hormones are present in an amount of 0.05 to 10% of the total composition.

12. The synergistic plant growth stimulant composition as claimed in claim 1, wherein the ratio of said composition and plant growth hormones varies from 1:10:0.5, 1:20:1, 1:50:1 and 1:100:1.

13. A process for the preparation of a synergistic plant growth stimulant composition according to claim 1, the process comprising: a) dissolving melatonin in DMSO (Dimethyl sulfoxide); b) dissolving nitrogenous compounds in water; c) mixing together the solutions of step (a) and step (b) in a ratio varying from 0.5:1 or 1:1 or 1:10 or 1:20; d) adding adjuvants/surfactants to the solution of step (c) in a ratio of 1:10, followed by making up to 300 ml volume per acre; and e) diluting the solution of step (d) with water for foliar application on crop.

14. A process for the preparation of a synergistic plant growth stimulant composition according to claim 10, the process comprising: a) dissolving melatonin in DMSO (Dimethyl sulfoxide); b) dissolving nitrogenous compounds in water; c) dissolving plant growth hormones in ethanol; d) mixing together the solutions of step (a), step (b) and step (c) in a ratio varying from 1:10:0.5 or 1:20:1 or 1:50:1 or 1:100:1; e) adding adjuvants/surfactants to the solution of step (d) in a ratio of 1:10, followed by making up to 300 ml volume per acre; and f) diluting the solution of step (e) with water for foliar application on crop.

15. A method of increasing resistance to water-logging stress of plants, eliminating ROS (reactive oxygen species), alleviating oxidative damages on a plant caused by water logging stress and biotic stresses, the method comprising spraying a synergistic plant growth stimulant composition according to claim 1 on leaves as foliar application or by mixing in soil as fertilizer application.

16. The synergistic plant growth stimulant composition as claimed in claim 1, wherein said compositions are useful for improving plant growth, enhancing yield, increasing resistance to water-logging stress of plants, eliminating ROS (reactive oxygen species) and alleviating oxidative damages on the plant caused by water logging stress and biotic stresses.

17. The synergistic plant growth stimulant composition as claimed in claim 10, wherein the ratio of said composition and plant growth hormones varies from 1:10:0.5, 1:20:1, 1:50:1 and 1:100:1.

18. The synergistic plant growth stimulant composition as claimed in claim 10, wherein said compositions are useful for improving plant growth, enhancing yield, increasing resistance to water-logging stress of plants, eliminating ROS (reactive oxygen species) and alleviating oxidative damages on the plant caused by water logging stress and biotic stresses.

19. A method of increasing resistance to water-logging stress of plants, eliminating ROS (reactive oxygen species), alleviating oxidative damages on a plant caused by water logging stress and biotic stresses, the method comprising spraying a synergistic plant growth stimulant composition according to claim 10 on leaves as foliar application or by mixing in soil as fertilizer application.

Description

BRIEF DESCRIPTION OF FIGURES

[0020] FIG. 1: Comparison of Absorption of micronutrients (in ppm) between composition comprising Melatonin and Nitrogenous compounds Vs. individual melatonin and control.

[0021] FIG. 2: Comparison of Phenol concentration (in ppm) between composition comprising Melatonin and Nitrogenous compounds Vs. control.

[0022] FIG. 3: Comparison of % of protein between composition comprising Melatonin and Nitrogenous compounds Vs. individual melatonin, individual nitrogenous compounds and control.

[0023] FIG. 4: Comparison of total protein content between composition comprising Melatonin and Plant growth hormones Vs. individual melatonin, individual Gibberellic acid and control.

DETAILED DESCRIPTION OF THE INVENTION

[0024] The invention will now be described in detail in connection with certain preferred and optional embodiments, so that various aspects thereof may be more fully understood and appreciated. The melatonin, nitrogenous compounds and plant growth hormones used in present composition are commercially procured or synthetically manufactured.

[0025] The present invention discloses a synergistic plant growth stimulant composition comprising Melatonin and Nitrogenous compounds along with suitable adjuvants and/or surfactant and/or excipients for improving plant growth, enhancing yield and decreasing biotic-abiotic stresses on plants.

[0026] The nitrogenous compounds used in the present synergistic composition are selected from urea and its derivatives, amino acids and its derivatives, proteins, protein digest and amines, either alone or in combination thereof.

[0027] Accordingly, in an embodiment, the present invention discloses a synergistic plant growth stimulant composition comprising Melatonin and Nitrogenous compounds such as urea and its derivatives selected from N, N-diformyl urea, N, N-diacetyl urea, di-substituted diaryl urea, dialkyl urea, diacyl urea, aryl urea, alkyl urea, urea formaldehyde (including oligomers and polymers) and urea acrylamide compounds either alone or in combination thereof.

[0028] These nitrogenous compounds improve plant growth (vegetative and flowering/reproductive stage) and quality produce with stress tolerance.

[0029] In another embodiment, the present invention discloses a synergistic plant growth stimulant composition comprising Melatonin and Nitrogenous compounds such as amino acids and its derivatives selected from alanine, valine, methionine, leucine, iso-leucine and phenylalanine, either alone or in combination thereof.

[0030] In another embodiment, the present invention discloses a synergistic plant growth stimulant composition comprising Melatonin and Nitrogenous compounds such as protein and/or protein hydrolysate and/or peptide combination and/or enzymes, either alone or in combination thereof.

[0031] In another embodiment, the present invention discloses a synergistic plant growth stimulant composition comprising Melatonin and Nitrogenous compounds such as amines selected from amidates, imidates, amine salts, amine oxides, polyamines, amines azides and aziridines, either alone or in combination thereof.

[0032] The amount of Melatonin used in the present synergistic composition ranges from 0.01% to 10% of the total composition.

[0033] The amount of Nitrogenous compounds used in the present synergistic composition ranges from 1 to 50% of the total composition.

[0034] The ratio of Melatonin and Nitrogenous compounds in the present synergistic composition varies from 0.5:1, 1:1, 1:10 and 1:20.

[0035] In another embodiment, the present invention disclose a synergistic plant growth stimulant composition comprising Melatonin and Nitrogenous compounds optionally in combination with plant growth hormones, for improving plant growth, enhancing yield and decreasing biotic-abiotic stresses on plants.

[0036] The plant growth hormones are selected from Gibberelic acid and abscisic acid either alone or in combination thereof.

[0037] The amount of plant growth hormones used in the present synergistic composition ranges from 0.05 to 10% of the total composition.

[0038] The ratio of Melatonin, Nitrogenous compounds and plant growth hormones in the present synergistic composition varies from 1:10:0.5, 1:20:1, 1:50:1, 1:100:1.

[0039] In another embodiment, a synergistic plant growth stimulant composition of the present invention comprises suitable adjuvants and/or surfactant and/or excipients selected from anionic, non-ionic and cationic surfactants viz., carboxy methyl cellulose (CMC), lignosulfonates, and the like.

[0040] The adjuvants and/or surfactant and/or excipients used in the synergistic composition are present in an amount ranges from 0.1 to 5% of the total composition.

[0041] The surfactant includes a wetting agent selected from poly oxy ethylene sorbitan ester, polyoxyethylene alkyl (or aryl) ether or polyoxyethylene fatty acid ester, anionic surfactants such as straight-chain alkylbenzene sulfonate, dialkylsulfosuccinate, lignin sulfonate or polynaphthyl sulfonate and high molecular compounds such as polyacrylates and a dispersant selected from naphthalene sulfonic acid formaldehyde condensate or lignin sulfonate etc.

[0042] The ratio of Melatonin in combination with Nitrogenous compounds and suitable adjuvants/surfactants/excipients is about 1:0.25.

[0043] The adjuvants/surfactants/excipients used in the present composition ensure uniform and wide spreading of the composition on the plant when applied, to give complete application of foliage by maintaining surface tension of leaf and molecule contact to the plant.

[0044] In another embodiment, a synergistic plant growth stimulant composition of the present invention comprising Melatonin with Nitrogenous compounds can be formulated in the form of solutions and/or powders and/or granules.

[0045] The present compositions can be prepared with different concentrations to give specific characteristics of antioxidant and nutritional property along with abiotic and biotic stress controlling capacity to the plants/crops.

[0046] In one preferred embodiment, the present invention provides a synergistic plant growth stimulant composition comprising Melatonin and Nitrogenous compounds in a ratio varies from 0.5:1 or 1:1 or 1:10 or 1:20 along with inert excipients such as an adjuvants and/or surfactant in an amount of 0.1 to 5%, for improving plant growth, enhancing yield and decreasing biotic-abiotic stresses on plants.

[0047] In one preferred embodiment, the present invention provides a synergistic plant growth stimulant composition comprising Melatonin and Nitrogenous compounds optionally in combination with plant growth hormones in a ratio varies from 1:10:0.5, 1:20:1, 1:50:1, 1:100:1 along with inert excipients such as an adjuvants and/or surfactant in an amount of 0.1 to 5%, for improving plant growth, enhancing yield and decreasing biotic-abiotic stresses on plants.

[0048] In yet another preferred embodiment, the present invention provides a synergistic plant growth stimulant composition comprising (i) Melatonin and Nitrogenous compounds and/or composition comprising (ii) Melatonin, Nitrogenous compounds and plant growth hormones that exhibits increase plant growth, enhance yield and decrease the biotic-abiotic stresses on plants, when compare with individual application of Melatonin and Nitrogenous compounds.

[0049] In another preferred embodiment, the present invention provides a process for preparation of said synergistic plant growth stimulant composition comprising Melatonin and Nitrogenous compounds comprising the steps of; [0050] a) Dissolving melatonin in DMSO (Dimethyl sulfoxide); [0051] b) dissolving nitrogenous compounds in water; [0052] c) mixing together the solutions of step (a) and step (b) in a ratio varies from 0.5:1 or 1:1 or 1:10 or 1:20; [0053] d) adding adjuvants/surfactants to the solution of step (c) in a ratio of 1:10, followed by making up to 300 ml volume per acre; and [0054] e) diluting the solution of step (d) with water for foliar application on crop.

[0055] In another preferred embodiment, the present invention provides a process for preparation of said synergistic plant growth stimulant composition comprising Melatonin and Nitrogenous compounds in combination with plant growth hormones comprising the steps of; [0056] a) Dissolving melatonin in DMSO (Dimethyl sulfoxide); [0057] b) dissolving nitrogenous compounds in water; [0058] c) dissolving plant growth hormones in ethanol; [0059] d) mixing together the solutions of step (a), step (b) and step (c) in a ratio varies from 1:10:0.5 or 1:20:1 or 1:50:1 or 1:100:1. [0060] e) adding adjuvants/surfactants to the solution of step (d) in a ratio of 1:10, followed by making up to 300 ml volume per acre; and [0061] f) diluting the solution of step (e) with water for foliar application on crop.

[0062] In another preferred embodiment, the present invention provides a method of increasing resistance to waterlogging stress of plants, eliminating ROS (reactive oxygen species), alleviating oxidative damages on the plant caused by water logging stress and biotic stresses by spraying (i) a synergistic plant growth stimulant composition comprising Melatonin and Nitrogenous compounds and/or (ii) a synergistic plant growth stimulant composition comprising Melatonin and Nitrogenous compounds with plant growth hormones; on leaves as foliar application or by mixing in soil as fertilizer application.

[0063] In yet another embodiment, the present invention discloses (i) a synergistic plant growth stimulant composition comprising Melatonin and Nitrogenous compounds and/or (ii) a synergistic plant growth stimulant composition comprising Melatonin and Nitrogenous compounds with plant growth hormones; wherein the amount of said composition(s) applied/sprayed on leaves as foliar application or by mixing in soil as fertilizer application is ranges from 0.3 kg to 1 kg per acre.

[0064] In yet another embodiment, the present invention discloses (i) a synergistic plant growth stimulant composition comprising Melatonin and Nitrogenous compounds and/or (ii) a synergistic plant growth stimulant composition comprising Melatonin and Nitrogenous compounds with plant growth hormones; wherein said composition(s) are useful for improving plant growth, enhancing yield, increasing resistance to water-logging stress of plants, eliminating ROS (reactive oxygen species) and alleviating oxidative damages on the plant caused by water logging stress and biotic stresses.

[0065] In yet another preferred embodiment, the present invention provides a synergistic plant growth stimulant composition comprising (i) Melatonin and Nitrogenous compounds and/or composition comprising (ii) Melatonin, Nitrogenous compounds and plant growth hormones, that exhibits increasing resistance to water-logging stress of plants, eliminating ROS (reactive oxygen species), alleviating oxidative damages on the plant caused by water logging stress and biotic stresses, when compare with individual application of Melatonin and Nitrogenous compounds.

[0066] In yet another embodiment, the present invention discloses that, the water logging stress of plants is eliminated by foliar application of a synergistic plant growth stimulant composition comprising (i) Melatonin and Nitrogenous compounds and/or composition comprising (ii) Melatonin, Nitrogenous compounds and plant growth hormones; wherein said composition provides antioxidants to plants and these antioxidants gives strength to plants, avoids cell damages occurs through ROS (reactive oxygen species) and develop stress tolerance capacity in plants.

[0067] In yet another embodiment, the present invention discloses that a synergistic plant growth stimulant composition comprising Melatonin and Nitrogenous compounds exhibits higher amount of micronutrients, Phenol concentration, % of protein and total protein content in plant as compared to individual melatonin, nitrogenous compounds, plant growth hormone and control respectively. The same is depicted in below Examples 9 to 12 and FIGS. 1 to 4.

[0068] In yet another embodiment, the present invention discloses a synergistic plant growth stimulant composition comprising Melatonin and Nitrogenous compounds optionally with plant growth hormones for improving plant growth, enhancing yield and decreasing the biotic-abiotic stresses on plants; wherein the plant is selected from the group consisting of cucurbit vegetables, peppers, potatoes, carrots, radishes, grapes, cole crops, leafy vegetables and tomatoes.

[0069] Some typical examples illustrating the embodiments of the present invention are provided; however, these examples are exemplary only and should not be regarded as limiting the elements of the present invention.

EXAMPLES

Example 1: Compositions Comprising Melatonin and Nitrogenous Compounds (Urea)

[0070]

TABLE-US-00001 Composition 1 Composition 2 Composition 3 Sr. Quantity Quantity Quantity No. Ingredients (gm/acre) (gm/acre) (gm/acre) 1. Melatonin 1.125 2.25 4.5 2. Urea 100 200 400 3. Adjuvants/ 5 10 15 surfactants Total weight 106.125 212.25 419.5 in g

Example 2: Compositions Comprising Melatonin and Nitrogenous Compounds (Diacetyl Urea)

[0071]

TABLE-US-00002 Composition 4 Composition 5 Composition 6 Sr. Quantity Quantity Quantity No. Ingredients (g/acre) (g/acre) (g/acre) 1. Melatonin 1.125 2.25 4.5 2. Diacetyl urea 25 50 100 3. Adjuvants/ 5 10 15 surfactants Total weight 31.25 62.25 119.5 in gm

Example 3: Compositions Comprising Melatonin and Nitrogenous Compounds (Di Aryl Urea)

[0072]

TABLE-US-00003 Composition 7 Composition 8 Composition 9 Sr. Quantity Quantity Quantity No. Ingredients (g/acre) (g/acre) (g/acre) 1. Melatonin 1.125 2.25 4.5 2. Di aryl urea 25 50 100 3. Adjuvants/ 5 10 15 surfactants Total weight 31.125 62.25 119.5 in gm

Example 4: Compositions Comprising Melatonin and Nitrogenous Compounds (Aryl Urea)

[0073]

TABLE-US-00004 Composi- Composi- Composi- tion 10 tion 11 tion 12 Sr. Quantity Quantity Quantity No. Ingredients (g/acre) (g/acre) (g/acre) 1. Melatonin 1.125 2.25 4.5 2. Aryl urea 25 50 100 3. Adjuvants/ 5 10 15 surfactants Total weight 31.125 62.25 119.5 in gm

Example 5: Compositions Comprising Melatonin and Nitrogenous Compounds (Diphenyl Urea)

[0074]

TABLE-US-00005 Composi- Composi- Composi- tion 13 tion 14 tion 15 Sr. Quantity Quantity Quantity No. Ingredients (gm/acre) (gm/acre) (gm/acre) 1. Melatonin 1.125 2.25 4.5 2. Diphenyl 25 50 100 urea 3. Adjuvants/ 5 10 15 surfactants Total weight 31.125 62.25 119.5 in gm

Example 6: Compositions Comprising Melatonin and Nitrogenous Compounds (Dimethyl Urea)

[0075]

TABLE-US-00006 Composi- Composi- Composi- tion 16 tion 17 tion 18 Sr. Quantity Quantity Quantity No. Ingredients (g/acre) (g/acre) (g/acre) 1. Melatonin 1.125 2.25 4.5 2. Dimethyl 25 50 100 urea 3. Adjuvants/ 5 10 15 surfactants Total weight 31.125 62.25 119.5 in gm

Example 7: Compositions Comprising Melatonin and Nitrogenous Compounds [N,N-Diformyl Urea (NN-DFU)]

[0076]

TABLE-US-00007 Composi- Composi- Composi- tion 19 tion 20 tion 21 Sr. Quantity Quantity Quantity No. Ingredients (g/acre) (g/acre) (g/acre) 1. Melatonin 1.125 2.25 4.5 2. NN-DFU 25 50 100 3. Adjuvants/ 5 10 15 surfactants Total weight 31.125 62.25 119.5 in gm

Example 8: Compositions Comprising Melatonin and Nitrogenous Compounds and Plant Growth Hormones

[0077]

TABLE-US-00008 Sr. Composition 22 Composition 23 No. Ingredients (g/acre) (g/acre) 1. Melatonin 1.125 4.5 2. Urea 100 400 3. Diacetyl urea 25 100 4. Diaryl urea 25 100 5. Aryl urea 25 100 6. Gibberellic acid 1 4 7. Abscisic acid 0.5 2 8. Adjuvants/ 5 20 surfactants Total weight 182.65 730.5 in gm

Example 9: Process for Preparation of Present Composition (without Plant Growth Hormones)

[0078] a) Dissolving 2.25 gm of melatonin in 10 ml of DMSO (Dimethyl sulfoxide); [0079] b) dissolving 100 gm of nitrogenous compounds (urea/Diacetyl urea/Diaryl urea/Aryl urea/Diphenyl urea/Dimethyl urea/N,N-diformyl urea) in 100 ml of water; [0080] c) mixing solutions of step (a) and step (b) in a ratio ranging from 0.5:1 or 1:1 or 1:10 or 1:20; [0081] d) adding 10 ml of adjuvants/surfactants to solution of step (c) in a ratio of 1:10, followed by making up to 300 ml volume per acre; and [0082] e) diluting the solution of step (d) with 200 lit of water for foliar application on crop.

Example 8: Process for Preparation of Present Composition (with Plant Growth Hormones)

[0083] a) Dissolving 2.25 gm of melatonin in 10 ml of DMSO (Dimethyl sulfoxide); [0084] b) dissolving 100 gm of nitrogenous compounds in 100 ml of water; [0085] c) dissolving 1.125 gm of plant growth hormones in 10 ml of ethanol; [0086] d) mixing solutions of step (a), step (b) and step (c) in a ratio ranging from 1:10:0.5, 1:20:1, 1:50:1, 1:100:1. [0087] e) adding 10 ml of adjuvants/surfactants to solution of step (d) in a ratio of 1:10, followed by making up to 300 ml volume per acre; and [0088] f) diluting the solution of step (e) with 200 lit of water for foliar application on crop.

[0089] Experimental Results:

Example 9: Micronutrient Analysis in Grain

[0090] Field trail results on PaddyArea 10-meter square 1 gm/liter Foliar Application

TABLE-US-00009 Zn Mg Mn Fe B Ca Sr. Example (Zinc) (Magnesium) (Manganese) (Iron) (Boron) (Calcium) No. Sample Name No. (ppm) (ppm) (ppm) (ppm) (ppm) (ppm) 1. Control - 28.00 530.00 38.00 216.00 29.00 311.00 Farmer Practice* 2. Melatonin 36.50 609.00 45.00 300.00 40.30 375.00 3. Melatonin + Example 5 43.50 726.00 45.00 371.00 42.90 457.00 Diphenyl urea 4. Melatonin + Example 7 58.10 828.00 54.00 439.00 46.00 455.00 NN DFU 5. Melatonin + Example 6 47.60 781.00 49.00 362.00 43.90 486.00 Dimethyl urea 6. Melatonin + Example 1 46.60 709.00 44.00 334.00 36.50 472.00 Urea *Farmer Practice - All kinds of N, P, K fertilizers and supplements

[0091] The values achieved in above table also demonstrated in the form of Figure. It is clear from above Example 9 and FIG. 1 provided below that the Composition comprising Melatonin and Nitrogenous compounds exhibits presence of higher amount of micronutrients as compare to individual melatonin and control.

Example 10: Phenol Concentration in Grain

[0092] Field trail results on PaddyArea 10-meter square 1 gm/liter Foliar Application

TABLE-US-00010 Sr. Example Phenol Conc No. Sample Name No. (ppm) 1. Control ? Farmer Practice* 16.2800 2. Melatonin + Diphenyl urea Example 5 21.6239 3. Melatonin + Urea Example 1 22.3578 4. Melatonin + Dimethyl urea Example 6 21.8991 5. Melatonin + NN DFU Example 7 24.2844 *Farmer Practice - All kinds of N, P, K fertilizers and supplements

[0093] The values achieved in above table also demonstrated in the form of Figure. It is clear from above Example 10 and FIG. 2 provided below that the Composition comprising Melatonin and Nitrogenous compounds exhibits presence of higher amount of Phenol concentration as compare to control.

Example 11: Percentage of Protein in Grain

[0094] Field trail results on PaddyArea 10-meter square 1 gm/liter Foliar Application

TABLE-US-00011 Sr. Example No. Sample Name No. % of Protein 1. Control ? Farmer Practice* 0.0060 2. Urea 0.0063 3. Diphenyl urea 0.0089 4. Dimethyl urea 0.0081 5. Melatonin + Diphenyl urea Example 5 0.0109 6. Melatonin + Dimethyl urea Example 6 0.0108 7. Melatonin + Urea Example 1 0.0112 8. NN DFU 0.0117 9. Melatonin + NN DFU Example 7 0.0142 *Farmer Practice - All kinds of N, P, K fertilizers and supplements

[0095] The values achieved in above table also demonstrated in the form of Figure. It is clear from above Example 11 and FIG. 3 provided below that the Composition comprising Melatonin and Nitrogenous compounds exhibits presence of higher amount of % of protein as compare to individual melatonin, individual nitrogenous compounds and control.

Example 12: Methionin with Plant Growth HormoneTotal Protein Content in Percentage in Grain

[0096] Field trail results on PaddyArea 10-meter square lgm/liter Foliar Application

TABLE-US-00012 Sr. Example Total Protein No. Sample Name No. content in % 1. Control ? Farmer Practice* 0.068 2. Melatonin 0.076 3. Gibberellic acid 0.084 4. Melatonin + Gibberellic acid 0.094 *Farmer Practice - All kinds of N, P, K fertilizers and supplements

[0097] The values achieved in above table also demonstrated in the form of Figure. It is clear from above Example 12 and FIG. 4 provided below that the Composition comprising Melatonin and Plant growth hormones exhibits presence of higher amount of total protein content as compare to individual melatonin, individual Gibberellic acid and control.

REFERENCES

[0098] .sup.ihttps://www.researchgate.net/publication/336535346_Biotic_and_Abiotic_Stresses_in_Plants [0099] .sup.iihttp://www.jnkvv.org/PDF/04042020191500Biotic%20stress.pdf [0100] .sup.iiihttp://www.jnkvv.org/PDF/04042020191500Biotic%20stress.pdf [0101] .sup.ivhttps://academic.oup.com/aobpla/article/9/4/plx025/3894178 [0102] .sup.vhttps://www.iclfertilizers.com/nitrogen