BREEDING METHOD FOR RAPIDLY STABILIZING COTTON DISTANT HYBRIDIZATION PROGENY

Abstract

A breeding method for rapidly stabilizing cotton distant hybridization progeny is disclosed. It belongs to the technical field of plant breeding. The disclosure is based on VSG (G. barbadense semi-compatible material) as female parent, general G. hirsutum materials as male parent of the cross to get F1 generation of G. barbadense-G. hirsutum hybrid materials, and treats the F1 generation of young bolls with 0.03-0.05% of colchicine aqueous solution to induce F2 generation to separate the stable intermediate materials or new varieties with excellent genes of both G. barbadense and G. hirsutum, so as to produce new cotton germplasm resources. The main innovation of the present disclosure lies in that it changes the previous treatment position and method of colchicine on cotton plant, and the disclosure is not limited by haploid and directly treats the cotton bolls on F1 cotton plants.

Claims

1. A breeding method for rapidly stabilizing cotton distant hybridization progeny, comprising: (1) Crossing an G. barbadense semi-compatible material VSG as the female parent and a G. hirsutum material as the male parent to obtain a F1 generation G. barbadense-G. hirsutum hybrid material; (2) Treating young bolls of the F1 generation G. barbadense-G. hirsutum hybrid material with colchicine to obtain a F2 generation G. barbadense-G. hirsutum hybrid material; and (3) Planting all successfully induced bolls, and carrying out 2?3 generations of continuous selection until the line is stable.

2. The breeding method for rapidly stabilizing cotton distant hybridization progeny of claim 1, wherein the G. barbadense semi-compatible material VSG is G. barbadense semi-compatible material VSg: G. barbadense with V7V7 bud yellow gene.

3. The breeding method for rapidly stabilizing cotton distant hybridization progeny of claim 1, wherein the G. hirsutum material is an early maturing G. hirsutum variety; Preferably, the G. hirsutum material is Lumianyan 28 and Jinke 178.

4. The breeding method for rapidly stabilizing cotton distant hybridization progeny of claim 1, wherein a specific operation of treating with colchicine in the step (2) is as follows: after flowering and boll formation of the plants of the F1 generation of G. barbadense-G. hirsutum hybrid material, selecting young bolls that are 10?15 days old after flowering to be processed, and using a 5 ml medical syringe to pierce into middle of the bolls from the base one-third of the young bolls and then injecting 2 ml of aqueous solution of colchicine with mass concentration of 0.03?0.05%.

5. The breeding method for rapidly stabilizing cotton distant hybridization progeny of claim 1, wherein a specific operation of the step (3) is as follows: after the treated cotton bolls with obvious expansion characteristics naturally mature and spit out fluff, retaining seeds individually, and then planting all the seedlings in the following year, selecting and harvesting G. hirsutum type single plants with early maturity, disease resistance and strong bolling from the progeny group, weighing a weight of a single boll after receiving and drying it, and carrying out an indoor seed test one by one, carding fiber length, ginning lint to calculating the lint percentage, selecting and remaining seed of single plant with boll weight more than 6.0 g, lint percentage greater than 42% and fiber length greater than 30 mm; observing each adult plant row that meets the conditions in the second year, conducting the indoor seed test again after the plants with the same appearance are mixed according to the rows, and breeding the plants that meet the breeding objectives to form a line, and carrying out line identification and variety comparison test.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the technical solutions in the embodiments or prior art of the present disclosure, the accompanying drawings to be used in the description of the embodiments or prior art will be briefly introduced below, and it will be obvious that the accompanying drawings in the following description are only embodiments of the present disclosure, and that for the person of ordinary skill in the field, other attachments can be obtained in accordance with the accompanying drawings provided without creative labor.

[0026] The accompanying drawings of FIG. 1 show a flowchart of the selection and breeding method of the present disclosure.

DETAILED DESCRIPTION OF THE EMBODIMENTS

[0027] The technical solutions in the embodiments of the present disclosure will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present disclosure, and it is clear that the described embodiments are only a part of the embodiments of the present disclosure and not all of the embodiments. Based on the embodiments in the present disclosure, all other embodiments obtained by a person of ordinary skill in the art without making creative labor are within the scope of protection of the present disclosure.

[0028] The agents required for the present disclosure are conventional experimental agents procured from commercially available sources; the unmentioned experimental methods are conventional experimental methods and will not be repeated herein.

Embodiment 1

[0029] The method of the present disclosure has been experimentally verified, and the method is feasible.

[0030] In 2012, the project team used Lumianyan 28 preferred line, Jinke 178 and other materials as the male parent to cross with VSG material respectively. In 2013, except for dark green leaves, all the other appearance phases of F1 generation plants were female parent type, with vigorous vegetative growth, tall plants, large leaves and deep cracks, large, long and milky white corolla, long oval bolls and thin bolls. The young bolls of 10-15 days old were treated by injecting 2 ml of aqueous solution of colchicine with a concentration of 0.05% per boll, and 5-8 bolls were treated in each combination. After treatment, except for part of the treated cotton bolls dried up and fall off, the appearance of most of the bolls had no change like untreated bolls, and only 1 or 2 bolls in each combination obviously expanded like G. hirsutum bolls. After these bolls naturally matured and spit out fluff, the seeds were retained individually. In 2013, the seeds of each boll were planted into rows, all of which showed the same appearance as G. hirsutum, but there were differences between individuals. In 2014?2016, single-plant selection of the progeny materials is continued, and in 2017, stable line Wan 116 is selected, and in 2020, stable line Wan 076 is selected, which has the characteristics of high and stable yield, high lint percentage, concentrated bolling and spitting out fluff, and high quality through variety test and identification from 2018 to 2022. Among them, Wan 116 lint cotton was tested by the Cotton Quality Supervision, Inspection and Testing Center of the Ministry of Agriculture, with an average fiber length of 30.3?31.6 mm in the upper part, which is 2?3 mm higher than that of Lumianyan 28, with a Micronaire value of about 4.6, which decreased by about 0.4.

[0031] In comparison, cotton semi-compatible materials and colchicine are used in previous studies and the disclosure, but the differences in the disclosure are also very obvious: First, the treatment objects are different, previous studies used semi-compatible materials as female parents and low-generation G. barbadense-G. hirsutum hybrid materials as male parents to induce the target haploid, then 0.03-0.05% colchicine was used to treat the target haploid seedlings or adult plants to obtain double G. barbadense-G. hirsutum hybrid stable homozygous lines to achieve the breeding goal. Second, the treatment position of plant body are different, the disclosure also uses the semi-compatible materials as the female parents and the G. barbadense-G. hirsutum hybrid low-generation materials as the male parents, but does not first induce haploid and then double the haploid, but directly uses the same dose of colchicine to treat the young on F1 cotton plants, and induces G. barbadense-G. hirsutum hybrid F2 and produced stable G. hirsutum-based phenotypic G. barbadense-G. hirsutum hybrid materials containing G. hirsutum and G. barbadense recombinant genes, so as to achieve the breeding goal.

[0032] Each embodiment in this specification is described in a progressive manner, and each embodiment focuses on the differences with other embodiments, and the same and similar parts of each embodiment can be referred to each other.

[0033] The above description of the disclosed embodiments enables a person skilled in the art to realize or use the present disclosure. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein may be realized in other embodiments without departing from the spirit or scope of the present disclosure. Accordingly, the present disclosure will not be limited to these embodiments shown herein, but will be subject to the broadest scope consistent with the principles and novel features disclosed herein.