TETRAPYRROLES CONJUGATES AS MRI CONTRAST AGENT

Abstract

The invention relates to the use of tetrapyrroles conjugated compounds as contrast agent in magnetic resonance imaging (MRI). In particular conjugates of natural hemin that can be embedded in gels and implants to image local delivery in MRI.

Claims

1-13. (canceled)

14. Tetrapyrroles conjugated compounds according to formula (I) ##STR00003## wherein, M consists of Fe, Ru, Os, Mn, Ni, Co Y consists F, Cl, Br, I, OH, pyridines, pyrazoles, imidazole, histidine, isothiocyanates, acetonitrile, methanol, wherein a linker with the formula (II) ##STR00004## is located between the tetrapyrroles and at least one at least partly water-soluble compound R.sub.1 and R.sub.2 wherein R.sub.1, R.sub.2 consists of OH, SH, NH.sub.2, HisGLyOMe, polyurethanes, polyacrylates, polystyrenes, polyvinyllactams, PGLA, polyacrylic acid, PVP, hyaloronic acid, arginine, lysine, histidine, PEG, polyether amines, polylysine, polyarginine, polyhistidine and their salts, solvates and solvates of these salts.

15. Tetrapyrroles conjugated compounds according to claim 14, wherein the linker between the tetrapyrroles and the at least partly water-soluble compound is containing ethylenediamine, amino ethanol, ethylene glycol, mercaptoethanol, ethanedithiol.

16. Tetrapyrroles conjugated compounds according to claim 15 with the formula (III) ##STR00005## and wherein R.sub.1 and R.sub.2 are consisting of OH, SH, NH.sub.2, HisGlyOMe, polyurethanes, polyacrylates, polystyrenes, polyvinyllactams, PGLA, polyacrylic acid, PVP, hyaloronic acid, arginine, lysine, histidine and PEG, polyether amines, polylysine, polyarginine, polyhistidine and their salts, solvates and solvates of these salts.

17. Tetrapyrroles conjugated compounds according to claim 16 with the formula (IV). ##STR00006##

18. Method for producing tetrapyrrole conjugated compounds, which contain within one molecule at least one tetrapyrroles compound, at least a partially water-soluble compound and their salts, solvates and solvates of these salts, characterized in that tetrapyrroles conjugated compounds with the formula (I) ##STR00007## meaning, M consists of Fe, Ru, Os, Mn, Ni, Co Y consists of F, Cl, Br, I, OH, pyridines, pyrazoles, imidazole, histidine, isothiocyanates, acetonitrile, methanol wherein a linker with the formula (II) ##STR00008## is located between the tetrapyrroles and at least one at least partly water-soluble compound R.sub.1 and R.sub.2 wherein R.sub.1, R.sub.2 consists of OH, SH, NH.sub.2, HisGlyOMe, polyurethanes, polyacrylates, polystyrenes, polyvinyllactams, PGLA, polyacrylic acid, PVP, hyaloronic acid, arginine, lysine and PEG, polyether amines, polylysine, polyarginine and their salts, solvates and solvates of these salts wherein cabodiimides are used as an activating agent for a coupling reaction of the tetrapyrroles compounds (1) with the linker (2) and wherein tetrapyrroles compounds are dissolved in an organic solvent and a coupling with a at least partly water soluble compound is carried out in acetone, PBS, MES, DMSO, DMF, DMAc, acetonitrile or mixtures of acetone, PBS, MES, DMSO, DMF, DMAc, acetonitrile.

19. Method for producing tetrapyrrole conjugated compounds according to the claim 5, wherein the activating agent is selected from the group consisting of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC), N,N-dicyclohexylcarbodiimide (DCC), 1-cyclohexyl-3-(3-dimethylaminopropyl)carbodiimide (CDC), 1-isopropyl-3-(3-dimethylaminopropyl)carbodiimide (IDC), 1-cyclohexyl-3-(2-morpholinoethyl)carbodiimide (CMC).

20. Method for producing tetrapyrrole conjugated compounds according to the claims 19, wherein the tetrapyrrole conjugates are dissolved in polar protic carrier liquids.

21. Method for producing tetrapyrrole conjugated compounds according to the claim 20, wherein the polar protic carrier liquids are PEG, PGLA or derivatives.

22. Use of tetrapyrroles conjugated compounds according to claim 14, which contain within one molecule at least one tetrapyrroles compound, at least one at least partially water-soluble compound and their salts, solvates and solvates of these salts as contrast agent in for MRI.

23. Use of tetrapyrroles conjugated compounds according to claim 14, wherein the tetrapyrroles compounds are located within an implant graft, especially a stent, guide wire or a catheter for MRI.

Description

EXAMPLES

Example 1

[0025] An example of a PEGylation reaction of a tetrapyrroles compound like hemin is described in the following instruction. Hemin (29 mg, 0.0445 mmol ) is dissolved in Tetrahydrofuran THF (10 mL) and triethylamine (0.6 mL) is added. Subsequently, the suspension is cooled to 0 C. and allowed to stir for 1 hour. Next, ethylchloroformate (0.425 mL, 4.46 mmol) is added and stirring was continued at 0 C. for another 2 hours. The mixture was filtered and the filtrate was treated with ethylenediamine (0.3 mL 4.48 mmol) and stirred at room temperature overnight. The mixture was concentrated in vacuo and used as such in the next reaction (I).

##STR00001##

[0026] Hemin derivative (3) was yielded with an amount of (15 mg, 50%). The hemin derivative was dissolved in chloroform and treated with succinymidyl PEG with different molecular weights gave after dialysis the desired PEGylated hemin derivative (4).

Example 2

[0027] An example of a further PEGylation reaction is described in (II) below. 0.15 m1Triethylamine is added to a suspension of 32.6 g Hemin (1) in 100 ml Tetrahydrofurane (THF)and suspension cooled down to 0 C. for 1 hour. Then 19.2 mg of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) is added to a suspension of Hemin to activate the carboxyl groups (2) within.

##STR00002##

[0028] An amine-PEG compound is added directly after the reaction to be further proceed at room temperature overnight with stirring. Treatment with amine-PEG with different molecular weights gave after dialysis the desired pegylated hemin derivative (3).

Example 3

[0029] An example of the process to solubilize hemin by treating it with amino acids as base is described below, similar to procedure as described by Ingberg et al. in U.S. Pat. No. 5,008,388 A. 6.52 g of crystalline hemin (0.01 M) and 3.48 g of crystalline L-arginine (0.02 M) were vigorously stirred for 10 to 15 hours in a beaker provided with a mechanical stirrer and containing a solvent mixture of 300 ml. of acetone and 20 ml. of water. The product formed was filtered off, washed with acetone, and dried. Yield ofhemin arginate: 9.5 g. (95%). Insoluble residue, determined by the method mentioned above: 0.14 g. (1.4%).

Example 4

[0030] An example of the process to solubilize hemin by treating it with amino acids as base is described below, similar to procedure as described by Ingberg et al. in U.S. Pat. No. 5,008,388 A. 6.52 g. of crystalline hemin (0.01 M) and 4.39 g. of 30 crystalline L-lysine (0.03 M) were treated as described in Example 2. Yield of hemin lysinate: 10.8 g. (99%). Insoluble residue: 0.020 g. (0.20%). It appears that the optimal molar proportion of hemin 35 to arginate is 1:3, because this gave the highest yield of hemin arginate, while the amount of insoluble residue was minimal.

Example 5

[0031] An example of incorporation of hemin derivatives in a hydrogel is detailed below:

[0032] Hyaluronic acid based hydrogels (1 g) was dissolved in PBS buffer (20 mL) and cooled to 0 C. Subsequently, EDC (2.5 g, 16.5 mmol) and NHS (0.345 g, 3.0 mmol) were added and allowed to stir for 15 minutes. Aminated hemin 2 (9.7 g, 15 mmol) was added and the reaction was continued for 24 h at room temperature. After dialysis (MCOW 2 k), the product was obtained and confirmed with UV-VIS.

Example 6

[0033] The derivatives of hemin complex structure with/without gel matrix as described vide supra are being used as CEST or chemical exchange saturation transfer agent for MR imaging. The complex hemin derivative with Hyaluronic acid gel within different coupling ratios have resulted in a novel MRI in vitro contrast and formulations are being applied to different cell lines for being imaged via MRI for detecting the presence of contrast agent presenting cell viability in the subject at the site, thereby visualizing contrast agent presenting cell activation in the subject e.g. fibroblasts, smooth muscle cells etc.

Toxicity Studies (In Vitro)

[0034] The toxicity of hemin water soluble derivatives (arginate-HA, lysinate-HL) and the PEGylated hemine (HP) was tested in HCT 116 cell line in concentrations up to 250 ug/ml up to 24 hours incubation. This concentration was chosen since it would be equivalent to the maximal plasma concentration after injection of 1 mmol/kg in a patient the common dose of cyclic Gd-contrast such as Omniscan.

[0035] In FIG. 1 the total cell number of HCT 116 cells (dead/alive) after 24 hours incubation with different concentrations of hemin derivatives is shown. Cisplatin (CDDP) was used as a positive control in a concentration of 50 mol/L.

[0036] In FIG. 2 the upper panel shows the amount of early apoptopic cells upon incubation of HCT 116 cells for 24 hours incubation with different concentrations of hemin derivatives is shown. CDDP was used a positive control in a concentration of 50 mol/L. The lower panel shows the amount of cells with nuclear condensation (significant apoptosis) and with membrane damage.

Scaffold Imaging

[0037] A set, 0.5% (w/v) collagen in 0.25M Acetic acid solution have been selected due to easy handling. Hemin Lysinate (HL) solutions in 1.5 ml PBS buffer were mixed with 1.5 ml collagen solution with final concentration of 0.1 mM to 1 mM HL and mixed at room temperature overnight. Two different phases occurred in the final composition so that homogeneity of the samples was not sufficient. The study discontinued.

[0038] In a second set of experiments, 0.5% (w/v) collagen tubular scaffolds without coil support were selected. Tubular shape was achieved after freeze-drying procedure. Tubular collagen scaffolds of 1.5 cm have introduced to HL solutions in 1.5 ml PBS buffer with final concentration of 0.1 mM to 1 mM HL and mixed at room temperature. Scaffold started to shrink and loose 3D structure immediately after introduction of HL. The study discontinued.

[0039] For the third study set, 0.5% (w/v) collagen tubular scaffolds with coil support were selected. The crosslinking procedure was complete and the end structures were stable in PBS buffer and pre-wetted for 30 mins before use. Tubular collagen scaffolds of 1.5 cm have introduced to HL solutions in 1.5 ml PBS buffer with final concentration of 0.1 mM to 1 mM HL and mixed at room temperature overnight. Supernatant solutions have collected from each sample to determine nonintegrated concentration of HL and labeled as HL-Day0 solutions. Following washing procedure with 1.5 ml PBS was performed in PBS solutions for the next 12 h and 24 h. Washing solutions have collected from each sample and labeled as HL-Day1 and HL-Day2.

[0040] For the fourth study set, the procedure described for the third set has followed with Hemin Arginate (HA) and 0.5% (w/v) collagen tubular scaffolds with coil. Cross-linked tubular collagen scaffolds of 1.5 cm have introduced to HA solutions in 1.5 ml PBS buffer with final concentration of 0.1 mM to 1 mM HA and mixed at room temperature overnight. Supernatant solutions have collected from each sample to determine nonintegrated concentration of HA and labeled as HA-Day0 solutions. Following washing procedure with 1.5 ml PBS was performed in PBS solutions for the next 12 h and 24 h. Washing solutions have collected from each sample and labeled as HA-Day1 and HA-Day2.

[0041] For the fifth study set, the procedure described for the third set has followed with Hemin-PEGs (HPEG) and 0.7% (w/v) collagen tubular scaffolds with coil due to sample availability. The HPEG samples were varied with different PEG lengths of 1 KDa, 5 KDa and 10 KDa. Cross-linked tubular collagen scaffolds in 1.5 ml PBS buffer have introduced to the three HPEG solutions in 1.5 ml PBS buffer with the maximum available concentration of each HPEG stock solutions and mixed at room temperature overnight. Supernatant solutions have collected from each sample to determine nonintegrated concentration of HPEG and labeled as HPEG-Day0 solutions. Following washing procedure with 1.5 ml PBS was performed in PBS solutions for the next 12 h and 24 h. Washing solutions have collected from each sample and labeled as HPEG-Day1 and HPEG-Day2.

[0042] Chemical Interaction: For the sixth study set, Hemin Lysinate (HL) was selected, the activation of carboxyl groups of HL was performed via EDC/NHS coupling in MES solution (Table 1) and dissolved each in 3 ml EDC/NHS solution* with content of 3.3 mM EDC. (*Ratio EDC:NHS was taken from a previous study with %5 (w/v) collagen tubular scaffolds as 0.5:2 which translated into weights as 15.625 mg EDC to 17.25 mg NHS for 25 ml MES buffer.)

TABLE-US-00001 TABLE 1 EDC/NHS coupling of Hemin Lysinate (HL) within a concentration range Amount of HL Sample Volume Sample Name Content (g) (ml) C L 0.1 1 mg/ml 0.003 3 C L 0.2 2 mg/ml 0.006 3 C L 0.25 2.5 mg/ml 0.0075 3 C L 0.5 5 mg/ml 0.015 3

[0043] In meantime, 0.5% (w/v) non-crosslinked tubular collagen scaffolds with coils incubated in MES buffer for 30 mins for the transition to wet state and MES buffer has removed before application. After the activation of carboxyl groups of HL, samples have introduced collagen scaffolds and mixed under rolling for 4 h to achieve homogenous labeling.

[0044] The final samples were in dark-brown color and homogeneous (except the sample 0.25%). A small amount of samples have collected for histology. The samples then fixed to %1 agarose for MR measurement. Samples were further investigated with 3T Clinical MRI (Philips) and 11.7T preclinical MRI (Bruker). Images are given in FIG. 1 and relaxation times of each sample are given in Table 2. In each scan two regions of interest (ROI) have been selected and mean values of multiple slices are listed. ROIs were chosen as one on scaffold region and one on agarose as control which scaffolds were embedded in.

TABLE-US-00002 TABLE 2 Relaxation Times of Collagen Scaffolds with/-out Hemin lysinate at 11.7T MR Sample Name T.sub.1 (ms) T.sub.2 (ms) T2* (ms) Conc (mM) C L 0.1 1823 36 187 2 30.7 0.5 1.3 Control (agarose) 2233 33 116 0.7 C L 0.20 2003 28 190 3 22.2 0.8 2.6 Control (agarose) 2519 25 108 0.4 C L 0.5 1908 29 194 3 20.9 0.8 6.5

MRI Conditions

[0045] Magnetic Resonance Imaging: Nuclear MR relaxometry of labeled scaffolds was performed in a clinical 3T whole-body MR scanner (Philips Achieva, Best, The Netherlands) using a knee coil (SENSE-flex-M; Philips, Best, The Netherlands) at room temperature. Longitudinal (T1) relaxation times were measured in 2D scan mode of turbo field echo sequences with a 10 flip angle refocusing pulses [TR=52 ms, TE=3-48 ms, number of echoes=10]. Transverse (T2) relaxation times were measured in 2D scan mode using a multi-slice, multi-shot spin-echo sequences with a 90 excitation pulse followed by a train of equally spaced 180 refocusing pulses [TR=1500 ms, TE=8-168 ms, number of echoes=20]. For T2* relaxometry, images at 32 echo times (TE range=3-99 ms) were acquired by using a multi-shot, multi-slice fast-field gradient-echo sequence [TR=196 ms, 3 ms interval between two echoes, slice thickness=2 mm, 30 flip angle]. T2 and T2* relaxation times (R2 and R2*) were calculated by fitting an exponential curve to the signal amplitudes as a function of the echo time (TE) for each segmented scaffold region using the Imalytics Preclinical Software. The exponential curve includes an offset to account for a signal plateau created by noise or a component with slow signal decay. Furthermore, T1- and T2-weighted images were acquired using a T1-weighted turbo-spin-echo (TSE) sequence [TR=9 ms, TE=700 ms, slice thickness=2 mm], and a T2- weighted TSE sequence [TR=1200 ms, TE=100 ms, slice thickness=2 mm]. The size and volume of the hydrogels were assessed based on T1- and T2-weighted TSE images using the Imalytics Preclinical Software. At least it can be seen in FIG. 3 the MR Images of Collagen Scaffolds with/-out HL at 3 T MR. T1-(a) and T2-(b) weighted images. 1 to 5 number tags are referring to HL labeled samples CL 0.1, CL 0.2, CL 0.25, CL 0.5 and Control CL scaffold without HL correspondingly.