DEVICE FOR EVALUATING NEUROVIRULENCE OF MUMPS VIRUS

Abstract

A device for evaluating the neurovirulence of a mumps virus, comprising: (I) a virus inoculation module, which is used for performing virus inoculation of a mumps virus to be evaluated on the lateral ventricle of a rat; (II) a processing module, which is used for performing vibration slicing on the fixed rat brain; (III) an imaging module, which is used for scanning and imaging the obtained rat brain slices; and (IV) an analysis module, which is used in the obtained imaging for calculating a neurovirulence index by using a formula I: the neurovirulence index=S1/S0×100 (formula I) according to the cross-sectional area S1 of a cavity formed by hydrocephalus in the longitudinal section of the rat brain and the total cross-sectional area S0 of the rat brain. Multiple results show that the results are stable, repeatability is high, and a wild strain may be distinguished from a vaccine strain. In addition, relative to a current monkey body neurovirulence model, animal cost and difficulty of operation are greatly reduced.

Claims

1. A device for evaluating the neurovirulence of mumps virus, which comprises: (I) a virus inoculation module for inoculating virus in the lateral ventricle of a rat with the mumps virus to be evaluated, and the rat is taken as sample i on the 20th to 30th days (preferably 22nd-28th days, more preferably 25th day) after virus inoculation; (II) a processing module for taking the mouse brain from the sample i and fixing it, and performing vibration slicing on the fixed rat brain; (III) an imaging module for scanning and imaging the rat brain slices obtained in the biological tissue slice module; (IV) an analysis module for counting the cross-sectional area S1 of the cavity formed by hydrocephalus in the longitudinal section of the rat brain and the total cross-sectional area S0 of the rat brain without the cerebellum in the imaging obtained by the imaging module, and calculating the neurovirulence index of the rat by Formula I.
Neurovirulence index=cross-sectional area S1 of cavity/total cross-sectional area S0 of brain×100  (Formula I)

2. The device of claim 1, wherein the rat is selected from the group consisting of a Wistar rat or a Lewis rat.

3. The device of claim 1, wherein the rat is 1 to 3 days old, preferably 1 to 2 days old, and more preferably 1 day old.

4. The device of claim 1, wherein in the virus inoculation module, the position of the virus inoculation is between the bregrna and the lambda in the rat brain, 1.5 to 2.5 mm (preferably 1.9 to 2.1 mm, more preferably 2 mm) on the left side of the sagittal suture, and 1.5 to 2.5 mm (preferably 1.9 to 2.1 mm, more preferably 2 mm) in depth.

5. The device of claim 1, wherein in the virus inoculation module, the virus inoculation amount is 10.sup.2 to 10.sup.4 CCID.sub.50, preferably 10.sup.2 to 10.sup.3 CCID.sub.50, more preferably 10.sup.2 CCID.sub.50.

6. The device of claim 1, wherein the processing module comprises the following elements: (a) an anesthesia element for anesthetizing sample i by injecting an anesthetic into sample i to obtain sample ii; (b) an optional cardiac perfusion element for cardiac perfusion of sample ii to obtain sample iii; (c) a fixing element for fixing the rat brain of sample iii; (d) embedding and slicing elements.

7. The device of claim 6, wherein the anesthetic element comprises an anesthetic and an anesthetic syringe.

8. The device of claim 7, wherein the anesthetic is injected by intraperitoneal injection or intramuscular injection.

9. The device of claim 6, wherein the fixing element comprises a fixing solution selected from the group consisting of paraformaldehyde, acetone, ethanol, and a combination thereof.

10. The device of claim 1, wherein in the analysis module, the statistical longitudinal section of the rat brain includes an area of 1 to 2.5 mm on the left side of the sagittal suture of the rat's left brain, preferably an area of 1.8 to 2.2 mm.

Description

DESCRIPTION OF THE DRAWINGS

[0053] FIG. 1 shows a schematic diagram of the position of virus inoculation in Example 1.

[0054] FIG. 2 shows a schematic diagram of the rat brain cavity in Example 1.

[0055] FIG. 3 shows a schematic diagram of a rat brain slice in Example 1.

[0056] FIG. 4 shows a diagram of the statistical method in Example 1.

[0057] FIG. 5 shows a diagram of the statistical results in Example 1.

[0058] FIG. 6 shows a schematic diagram of the effects of two slicing methods in the comparative example.

DETAILED DESCRIPTION OF THE PREFERRED

[0059] After extensive and in-depth research and a large number of screening, the inventors have developed a rat model for evaluating the neurovirulence of mumps virus rats with stable results, convenient operation and objective evaluation for the first time. The experimental results show that in the construction of a rat model for evaluating the neurovirulence of mumps virus, the processing step of vibration section is used to replace the step of paraffin section-HE staining in the traditional method, and the direct analysis after the section can eliminate the HE staining step, which shortens the processing time by 2 to 3 days, and significantly reduces the difficulty of operation. In addition, in the model of the present invention, the use of Wistar rats instead of Lewis rats also solves the problem that Lewis rats are scarce and difficult to obtain. On this basis, the present invention is completed.

[0060] The main advantages of the present invention include:

[0061] 1) Strong repeatability. Multiple results show that the neurovirulence model can distinguish wild strains, partially attenuated strains and attenuated strains.

[0062] 2) Objectivity and accuracy. The influence of artificial operations on the results during the section processing is excluded, which objectively reflects the cavity of brain slices.

[0063] 3) Easy to operate. The rat brain can be directly statistically analyzed after slicing, and no other processing is required.

[0064] 4) Low cost. Replacing monkeys with rats greatly reduces animal costs and feeding costs.

[0065] The present invention is further explained below in conjunction with specific example. It should be understood that these examples are only for illustrating the present invention and not intend to limit the scope of the present invention. The conditions of the experimental methods not specifically indicated in the following examples are usually in accordance with conventional conditions as described in Sambrook et al., Molecular Cloning: A Laboratory Manual (New York: Cold Spring Harbor Laboratory Press, 1989), or according to the conditions recommended by the manufacturer. Unless otherwise stated, percentages and parts are percentages by weight and parts by weight.

Example 1: Establishment of the Rat Model for Evaluating Neurovirulence of Mumps Virus

[0066] 1.1 Animal and Virus Dilution

[0067] Four SPF Wistar pregnant rats were selected and randomly divided into 4 groups, namely A, B, C and D (the number of suckling rats in each group was not less than 10). Among them, group A was the control group inoculated with DMEM medium, group B was inoculated with vaccine strains, group C was inoculated with wild mumps virus strains passaged by Vero cells for 10 times, and group D was inoculated with wild mumps virus strains. In groups B, C and D, the virus titer was diluted with DMEM medium to 4.0 lg CCID.sub.50/mL.

[0068] 1.2 Virus Inoculation

[0069] After the mother rats gave birth, the 1-day-old suckling rats were fixed, and the sterilized 20 μL micro-syringe (size 27G) was used to absorb 10 μL virus solution. The injection position is between the bregrna and the lambda in the rat left brain, 2 mm on the left side of the sagittal suture, and 2 mm in depth. Each virus was inoculated with 10 rats, and the group of blank control was injected with 10 μL DMEM medium in the same operation.

[0070] 1.3 Anesthesia and Cardiac Perfusion in Rat

[0071] On the 25th day after virus inoculation, 1 mL 10% chloral hydrate solution was absorbed and injected intraperitoneally into rats. After 2 minutes, the rats were anesthetized and fixed in supine position, and the heart was perfused with 30 mL of PBS and 30 mL of 4% paraformaldehyde.

[0072] 1.4 Brain Harvesting and Fixation

[0073] The skull was carefully removed and the rat brain was harvested as intact as possible, including the brain stem. The rat brains were immersed in 4% paraformaldehyde solution and placed in a refrigerator at 4° C. for 24 hours.

[0074] 1.5 Embedding and Slicing of Rat Brain

[0075] A certain amount of agarose was weighted and heated to dissolve in PBS for the preparation of 5% agarose gel. When the agarose solution is slightly cooled but not solidified, it was poured into a 6-well plate and the rat brain was placed horizontally. During the solidification of the agarose solution, adjust and maintain the horizontal posture of the rat brain.

[0076] After 2 hours, the rat brain that has been embedded in agarose gel was taken out of the 6-well plate, and the rat brain was cut in half vertically along the sagittal suture of the rat brain with a blade. The part containing the left hemisphere was cut into a cuboid glue block containing the intact left brain, and it was fixed on the microtome stage with glue. After the glue has solidified, PBS was poured into the carrier tank until overflowing the agarose gel.

[0077] Set the starting position of slicing and other parameters to start the slicing (the forward speed of the blade is set to 1.50 mm/s, the amplitude is set to 0.75 mm, and the thickness of slice is set to 80 μm). An intact slice at a distance of 2 mL from the sagittal suture of the rat brain was selected, and the brain slice was removed from the PBS with a writing brush and attached to a glass slide. Maintain the natural shape of the slice and place the slide in the scanner for scanning and photographing.

[0078] 1.6 Statistical Analysis

[0079] The software image pro plus was used to analyze the slice photos of each group and calculate the neurovirulence index of each group.

[0080] After the rat brains in group A and group B were fixed, some of the rat brains had obvious cavities after incision, as shown in FIG. 2. After vibration slicing, some rat brain slices in each group are shown in FIG. 3. The cavities in group A (DMEM group) and group B (vaccine strain group) are not obvious, while obvious cavities are found in some rat brains in group C (wild strain passage group) and group D (wild strain group). The final neurovirulence index statistical results are shown in FIG. 5. The average score of group A is 0.15, the average score of group B is 0.31, the average score of group C is 1.63, and the average score of group D is 5.66. There is no significant difference between groups A and B, but there is significant difference between groups B, C and D (P<0.05).

Comparative Example: Effect Test of the Rat Model for Evaluating the Neurovirulence of Mumps Virus-Comparison of Brain Tissue Processing Methods

[0081] 2.1 Animals and Grouping

[0082] Two SPF Wistar pregnant rats were divided into E and F groups. Group E was a group of the wild mumps virus strain, in which half of the rat brains were treated with vibration slicing, and the rest rat brains were treated with paraffin section-HE staining. Group F was a DMEM control group, and the postprocessing method after harvesting brain was consistent with that of group E.

[0083] 2.2 Virus Inoculation

[0084] The titer of wild mumps virus strain was diluted to 4.0 lg CCID.sub.50/mL. The 1-day-old suckling rat was fixed, and the sterilized 20 μL micro-syringe (size 27G) was used to absorb 10 μL virus solution to inoculate the left ventricle of the suckling rat. The inoculation position is between the bregrna and the lambda in the rat left brain, 2 mm on the left side of the sagittal suture, and 2 mm in depth. The group of blank control was injected with 10 μL DMEM medium in the same operation.

[0085] 2.3 Anesthesia and Cardiac Perfusion in Rat

[0086] On the 25th day after virus inoculation, 1 mL 10% chloral hydrate solution was absorbed and injected intraperitoneally into rats. After 2 minutes, the rats were anesthetized and fixed in supine position, and the heart was perfused with 30 mL of PBS and 30 mL of 4% paraformaldehyde.

[0087] 2.4 Brain Harvesting and Fixation

[0088] The skull was carefully removed and the rat brain was harvested as intact as possible, including the brain stem. The rat brains were immersed in 4% paraformaldehyde solution and placed in a refrigerator at 4° C. for 24 hours.

[0089] 2.5 Paraffin Sections and Hematoxylin-Eosin (HE) Staining

[0090] 2.5.1 Dehydration and Transparency. A suitable size of brain tissue was cut and placed in a disposable plastic dehydration embedding box, and washed with running water for 1 hour. Ethanol gradient dehydration and xylene transparency were performed at room temperature (25° C.), specifically, 50% ethanol for 2 hours.fwdarw.60% ethanol for 2 hours.fwdarw.75% ethanol for 2 hours.fwdarw.85% ethanol for 2 hours.fwdarw.95% ethanol for 1 hour.fwdarw.absolute ethanol for 1 hour.fwdarw.xylene for 20 minutes. The result of transparency was observed. Suck up the liquid on the peripheral surface of the embedding box, and transfer it into the dipping wax.

[0091] 2.5.2 Waxdip and embedding. The rat brain tissue block was immersed in paraffin solution at 60° C. for 2 hours, and then a certain wax solution is injected into the metal embedding mold, put the brain tissue with the section face downward to avoid generating bubbles.

[0092] 2.5.3 Slicing and baking. The embedded tissue block was repaired and fixed, and the slice thickness was set to 8 μm for slicing. The wax slices with intact section were flattened in warm water at 42° C. with a writing brush, and laid flat on the glass slide. The wax slices were baked at 60° C. for 2 hours and then stored in a slicing box in order.

[0093] 2.5.4 Hematoxylin-eosin (HE) staining. The slices were put into xylene for 40 minutes, absolute ethanol for 10 minutes, 75% alcohol for 5 minutes, and washed with tap water. The slices were put into hematoxylin staining solution for 3-5 minutes and washed with tap water. The slices were successively dehydrated with gradient alcohol of 85% and 95% for 5 minutes respectively, and stained with eosin staining solution for 5 minutes. The slices were successively put into anhydrous ethanol for 15 minutes, xylene for 10 minutes to transparent, and then sealed with neutral gum.

[0094] 2.6 Vibration Slicing

[0095] 2.6.1 Rat Brain Embedding

[0096] A certain amount of agarose was weighted and heated to dissolve in PBS for the preparation of 5% agarose gel. When the agarose solution is slightly cooled but not solidified, it was poured into a 6-well plate and the rat brain was placed horizontally. During the solidification of the agarose solution, adjust and maintain the horizontal posture of the rat brain.

[0097] 2.6.2 Slicing

[0098] After 2 hours, the rat brain that has been embedded in agarose gel was taken out of the 6-well plate, and the rat brain was cut in half vertically along the sagittal suture of the rat brain with a blade. The part containing the left hemisphere was cut into a cuboid glue block containing the intact left brain, and it was fixed on the microtome stage with glue. After the glue has solidified, PBS was poured into the carrier tank until overflowing the agarose gel.

[0099] Set the starting position of slicing and other parameters to start the slicing (the forward speed of the blade is set to 1.50 mm/s, the amplitude is set to 0.75 mm, and the thickness of slice is set to 80 μm). An intact slice at a distance of 2 mL from the sagittal suture of the rat brain was selected, and the brain slice was removed from the PBS with a writing brush and attached to a glass slide.

[0100] 2.7 Image Acquisition and Analysis

[0101] After taking photos of the slices, the results of vibration slicing and paraffin section-HE staining in the E and F groups were compared and analyzed.

[0102] As shown in FIG. 6, after vibration slicing treatment and paraffin section-HE staining treatment, in the wild virus strain group, cavities in the cross-section of brain can be seen, but there are no cavities in the DMEM group. There is no significant difference in the final statistical results between the two processing methods, but the paraffin section-HE staining processing cycle is long and the operation is complicated, which may easily lead to shrinkage, folding, and deformation of the slice, change the natural shape of the slice, and artificially change the cross-section of the brain, which interferes with the analysis.

[0103] All literatures mentioned in the present application are incorporated by reference herein, as though individually incorporated by reference. In addition, it should be understood that after reading the above teaching content of the present invention, various changes or modifications may be made by those skilled in the art, and these equivalents also fall within the scope as defined by the appended claims of the present application.