ACYL-ACP THIOESTERASES AND MUTANTS THEREOF

Abstract

Novel plant acyl-ACP thioesterase genes of the FatB and FatA classes and proteins encoded by these genes are disclosed. The genes are useful for constructing recombinant host cells having altered fatty acid profiles. Expression of the novel and/or mutated FATB and FATA genes is demonstrated in oleaginous microalga host cells. Furthermore, a method for producing an oil elevated in one or more of C12:0, C14:0, C16:0, C18:0 and/or C18:1 fatty acids includes transforming a cell with novel and/or mutated FATB and/or FATA genes, e.g., having an N-terminal deletion. The cells produce triglycerides with altered and useful fatty acid profiles.

Claims

1. A cDNA or nucleic acid construct comprising a polynucleotide sequence encoding a heterologous regulatory element and a FatB acyl-ACP thioesterase gene operable to produce an altered fatty acid profile in an oil produced by a cell expressing the nucleic acid construct, wherein the FatB gene expresses a protein having an amino acid sequence having at least 65% identity to SEQ ID NO: 40 or SEQ ID NO: 42.

2. The cDNA or nucleic acid construct of claim 1, wherein the acyl-ACP thioesterase coding sequence comprises at least 65% identity to SEQ ID NO: 44 or any equivalent sequences by virtue of the degeneracy of the genetic code.

3. (canceled)

4. The nucleic acid construct of claim 1, further comprising a polynucleotide encoding a plastid targeting peptide with at least 65% identity to SEQ ID NO: 37.

5. (canceled)

6. A host cell capable of expressing the cDNA or nucleic acid construct of claim 1 so as to produce a triglyceride oil having an altered composition relative to a control cell without the construct, the oil optionally having an increase in C8-C12 fatty acids.

7. A host cell capable of expressing the cDNA or nucleic acid construct of claim 1 so as to produce a triglyceride oil having an altered composition relative to a control cell without the construct, the oil optionally having an increase in C10 and C12 fatty acids.

8. The host cell of claim 6, wherein the host cell is selected from the group consisting of a plant cell, a microbial cell, and a microalgal cell.

9. A method of producing a recombinant cell that produces an altered fatty acid profile, the method comprising transforming a cell with a cDNA or nucleic acid construct of claim 1.

10. A host cell produced by the method of claim 9.

11. The host cell of claim 10, wherein the host cell is selected from the group consisting of a plant cell, a microbial cell, and a microalgal cell.

12. A method for producing an oil or oil-derived product, the method comprising cultivating a host cell of claim 6 and extracting oil produced thereby, optionally wherein the cultivation is by heterotrophic growth on sugar.

13. The method of claim 12, further comprising producing a fatty acid, fuel, chemical, food, or other oil-derived product from the oil.

14. (canceled)

15. An oil produced by the method of claim 12, optionally having a fatty acid profile comprising at least 20% C8, C10, C12, C14, C16 or C18 fatty acids.

16. The oil of claim 15, wherein the oil is produced by a microalgae, has a microalgal sterol profile, and optionally, lacks C24-alpha sterols.

17. (canceled)

18. A method for producing an oil, the method comprising: (a) providing a plastidic, oleaginous cell, optionally a microbial cell, the cell expressing a functional, acyl-ACP thioesterase gene encoded by a FATB gene having a deletion mutation in a region corresponding to the region coding for amino acids 66-98 of SEQ ID NO: 8; (b) cultivating the cell to produce a cell-oil; and (c) isolating the cell-oil from the cell.

19. The method of claim 17, wherein the cell-oil is enriched in C12 due to the deletion.

20. The method of claim 18 wherein the FATB gene encodes a protein with at least 65% sequence identity to SEQ ID NO: 42.

21. (canceled)

22.-38. (canceled)

39. The oil produced by the method of claim 19, wherein the fatty acid profile of the cell-oil comprises at least 5% C12 fatty acids.

40. The oil produced by the method of claim 39, wherein the fatty acid profile of the cell-oil comprises at least 10% C12 fatty acids.

41. The oil produced by the method of claim 40, wherein the fatty acid profile of the cell-oil comprises at least 15% C12 fatty acids.

Description

EXAMPLE 1

Discovery of Novel FATB Sequences

[0096] RNA was extracted from dried plant seeds and submitted for paired-end sequencing using the Illumina Hiseq 2000 platform. RNA sequence reads were assembled into corresponding seed transcriptomes using the Trinity software package and putative thioesterase-containing cDNA contigs were identified by mining transcriptomes for sequences with homology to known thioesterases. In some cases, these in silico identified putative thioesterase cDNAs were verified by direct reverse transcription PCR analysis using seed RNA and primer pairs targeting full-length thioesterase cDNAs. The resulting amplified products were cloned and sequenced de novo to confirm authenticity of identified thioesterase genes and to identify sequence variants arising from expression of different gene alleles or diversity of sequences within a population of seeds. For some sequences, a high-confidence, full-length transcript was assembled using Trinity and reverse transcription was not deemed to be necessary. The resulting amino acid sequences of all new putative FATB thioesterases were subjected to phylogenetic analyses using published full-length (Mayer and Shanklin, 2007) and truncated (THYME database) sequences as well as an extensive in-house phylogeny developed at Solazyme from FATB sequences identified in numerous oilseed transcriptomes. The in-house phylogeny comprising the acyl-ACP FATB thioesterases allows for prediction, in many cases, of the midchain specificity for each thioesterase; the FATBs predicted to be involved in biosynthesis of C8-C12 fatty acids were pursued.

[0097] The amino acid sequence and nucleic acid CDSs (native to the plant and codon optimized for Prototheca moriformis) of the novel FatB genes with and without their N-terminal plastid targeting peptides are shown in Table 1, above.

EXAMPLE 2

Expression of Transforming Vectors Expressing Acyl-ACP FATB Thioesterases

[0098] The nine acyl-ACP FATB thioesterase genes of Example 1 were synthesized in a codon-optimized form to reflect Prototheca moriformis (UTEX 1435) codon usage. A representative transforming construct and the sequence of the FATB enzymes is provided in SEQ ID NO: 38, using CcrasFATB1 as an example. The new thioesterases were synthesized with a modified transit peptide from Chlorella protothecoides (Cp) (SEQ ID NO: 40) in place of the native transit peptide. The modified transit peptide derived from the CpSAD1 gene, CpSAD1tp_trimmed, was synthesized as an in-frame, N-terminal fusion to the FATB thioesterases in place of the native transit peptide.

[0099] Transgenic strains were generated via transformation of the base strain S7485 with a construct encoding 1 of the 12 FatB thioesterases. The construct pSZ5342/D4219 encoding CcrasFATB1 is shown as an example, but identical methods were used to generate each of the remaining 11 constructs encoding the different respective thioesterases. Construct pSZ5342 can be written as THI4A_5::CrTUB2-ScSUC2-PmPGH:PmSAD2-2ver3-CpSAD1tp_trmd:CcrasFATB1-CvNR-THI4A_3. The relevant restriction sites in the construct from 5-3, BspQ1, KpnI, BamHI, EcoRV, SpeI, XhoI, SacI, BspQ1, respectively, are indicated in lowercase, bold, and underlined. BspQ1 sites delimit the 5 and 3 ends of the transforming DNA. Bold, lowercase sequences at the 5 and 3 end of the construct represent genomic DNA from UTEX 1435 that target integration to the THI4A locus via homologous recombination. Proceeding in the 5 to 3 direction, the selection cassette has the C. reinhardtii -tubulin promoter driving expression of the S. cerevisiae gene SUC2 (conferring the ability to grow on sucrose) and the P.moriformis PGH gene 3 UTR. The promoter is indicated by lowercase, boxed text. The initiator ATG and terminator TGA for ScSUC2 are indicated by bold, uppercase italics, while the coding region is indicated with lowercase italics. The 3 UTR is indicated by lowercase underlined text. The spacer region between the two cassettes is indicated by upper case text. The second cassette containing the codon optimized CcrasFATB1 gene from Cuphea crassiflora fused to the heterologous C. protothecoides SAD1 plastid-targeting transit peptide, CpSAD1tp_trimmed, is driven by the P.moriformis SAD2-2ver3 pH5-responsive promoter and has the Chlorella vulgaris Nitrate Reductase (NR) gene 3 UTR. In this cassette, the PmSAD2-2ver3 promoter is indicated by lowercase, boxed text. The initiator ATG and terminator TGA for the CcrasFATB1 gene are indicated in bold, uppercase italics, while the coding region is indicated by lowercase italics. The 3 UTR is indicated by lowercase underlined text.

[0100] The sequence for all of the thioesterase constructs is identical with the exception of the encoded thioesterase. The full sequence for pSZ5342/D4219 integrating construct (SEQ ID NO: 38) is provided.

[0101] Constructs encoding heterologous FATB genes were transformed into a high-lipid-producing Prototheca strain and selected for the ability to grow on sucrose. Transformations, cell culture, lipid production and fatty acid analysis were all carried out as in WO2013/158938. Multiple transformations were performed. The fatty acid profiles of the strain with the highest C10 (for the first 6 genes listed), or C12 production (for the remaining genes) is reported in Table 8.

TABLE-US-00008 TABLE 8 Fatty acid profiles of top performing strain from each transformation (%; primary lipid) Species Gene Name C8:0 C10:0 C12:0 C14:0 C16:0 C18:0 C18:1 C18:2 C18:3 Cuphea crassiflora CcrasFATB1 0 4 1 3 35 3 47 5 0 Cuphea koehneana CkoeFATB3 0 9 2 3 32 3 45 5 0 Cuphea leptopoda CleptFATB1 0 6 1 3 34 4 46 5 0 Cuphea angustifolia CangFATB1 0 4 1 3 34 3 48 5 1 Cuphea llavea CllaFATB1 0 9 1 4 33 3 43 5 1 Cuphea lophostoma ClopFATB1 0 7 1 4 33 3 45 5 1 Sassafras albidum SalFATB1 0 0 7 3 32 4 47 5 1 Sassafras albidum SalFATB2 0 0 0 2 36 3 52 5 1 Lindera benzoin LbeFATB1 0 1 11 3 23 2 53 6 1 None (Parent strain) None 0 0 0 2 38 4 48 5 1

[0102] The six thioesterases from the Lythraceae cluster all display specificity towards C10:0 fatty acids: CcrasFATB1, which exhibits 4% C10:0 and 1% C12:0 fatty acid levels; CkoeFATB3, which exhibits 9% C10:0 and 2% C12:0 fatty acid levels; CleptFATB1, which exhibits 6% C10:0 and 1% C12:0 fatty acid levels; CangFATB1, which exhibits 4% C10:0 and 1% C12:0 fatty acid levels; CllaFATB1, which exhibits 9% C10:0 and 1% C12:0 fatty acid levels; and, ClopFATB1, which exhibits 7% C10:0 and 1% C12:0 fatty acid levels.

[0103] SalFATB1 and LbeFATB1, both of the Lauraceae family, exhibit substantial activity towards C12:0 fatty acids.

EXAMPLE 3

FATB Deletion Mutants of Lauraceae FATB Genes

[0104] Transforming vectors for deletion variants, of SalFATB1, and LbeFATB1, known respectively as SalFATB 1 a and LbeFATB1a, were synthesized, using the expression cassette and transit-peptide described in Example 2. The deletion variants had deletions in the region corresponding to amino acids 66-98 of the SalFATB2 gene (SEQ ID NO: 8). The constructs were codon-optimized to reflect UTEX 1435 codon usage. Transformations, cell culture, lipid production and fatty acid analysis were carried out as in Example 2. Constructs encoding heterologous FATB genes were transformed into a Prototheca moriformis strain and selected for the ability to grow on sucrose. The results for the two novel FATB thioesterases are displayed in Table 9.

TABLE-US-00009 TABLE 9 Fatty acid profiles of strains expressing deletion mutants of fatty acyl-ACP FATB genes (FATB1a) compared to wildtype genes lacking the deletion (FATB1). Gene C8:0 C10:0 C12:0 C14:0 C16:0 C18:0 C18:1 C18:2 C18:3 SalFATB1 0 0 7 3 32 4 47 5 1 SalFATB1a 0 0 15 3 27 3 45 5 1 LbeFATB1 0 1 11 3 23 2 53 6 1 LbeFATB1a 0 3 28 5 18 2 37 4 0

[0105] SalFATB1 and LbeFATB1, both of the Lauraceae family, exhibit substantial activity towards C12:0 fatty acids. SalFATB1a, which has a deletion of the 32 amino acids LFAVITTIFSVAEKQWTNLEWKPKPKPRLPQL (SEQ ID NO: 47), produced up to 15% C12:0 compared to 7% produced by the wild-type SalFATB1. The mean C12:0 level in SalFATB1a was 8.3% compared to 3.7% in SalFATB1, demonstrating a greater than 2-fold increase in activity upon deletion of the 32 amino acids. LbeFATB1a, which had a deletion of the 28 amino acids LLTVITTIFSAAEKQWTNLERKPKPPHL (SEQ ID NO: 48), produced up to 28% C12:0 compared to 11% produced by the wild-type LbeFATB1. The mean C12:0 level in LbeFATB1a is 17.2% compared to just 5.7% in LbeFATB1, demonstrating a greater than 3.0-fold increase in activity upon deletion of the 28 amino acids. The data suggest that deletion of those amino acids significantly improves (e.g., by 2-3 fold) the C12 activity of two other Lauraceae family thioesterases, SalFATB1 and LbeFATB1.

EXAMPLE 4

Additional Deletion Mutants from FATB Genes from Lythraceae

[0106] P. moriformis was transformed with additional deletion mutants of Lythraceae FATB genes above for Lauraceae FATB genes. Two deletion mutants were identified that showed elevated midchain (C8-14) fatty acid levels in cell-oil extracted from the microalga relative an equivalent transformation lacking the deletion. These are listed in Table 9, above in which they appear as CpauFATB128 and ChFATB227. Fatty acid profiles obtained in the P. moriformis model system are reported below in Table 10. ChFATB227 demonstrated an increase in C8 and C10 fatty acids when compared to the wild-type, elaborating an average of 3.8% C8:0 and 11.5% C10:0 compared to 2.7% C8:0 and 8.0% C10:0, respectively. CpauFATB128 demonstrates an increase in C10, C12 and C14 fatty acids when compared to the wild-type, elaborating an average of 7.6% C10:0 compared to 4.1% C10:0, respectively.

TABLE-US-00010 TABLE 10 Fatty acid profiles of cell-oil from P. moriformis transformed with Lythraceae FATB deletion mutants for top performing transformants (mean given in parenthesis). Mutant C8:0 C10:0 C12:0 C14:0 C16:0 C18:0 C18:1 C18:2 C18:3 CpauFATB1 0 (0.0) 9 (4.1) 1 3 31 2 45 6 1 (0.6) (2.7) CpauFATB128 0 (0.0) 14 (7.6) 2 4 30 3 42 5 1 (1.1) (3.0) ChFATB2 7 (2.7) 16 (8.0) 0 2 21 3 44 5 1 (0.2) (2.0) ChFATB227 9 (3.8) 20 (11.5) 0 1 17 2 45 5 0 (0.2) (1.8)

EXAMPLE 5

Modify Brassica napus Thioesterase (BnOTE) Enzyme Specificity by Site Directed Mutagenesis

[0107] In the example below, we demonstrate the ability of modifying the enzyme specificity of a FATA thioesterase originally isolated from Brassica napus (BnOTE, accession CAA52070), by site directed mutagenesis targeting two amino acids positions (D124 and D209).

[0108] To determine the impact of each amino acid substitution on the enzyme specificity of the BnOTE, the wild-type and the mutant BnOTE genes were cloned into a vector enabling expression within the lower palmitate P. moriformis strain S8588. The Saccharomyces carlsbergensis MEL1 gene (Accession no: AAA34770) was utilized as the selectable marker to introduce the wild-type and mutant BnOTE genes into FAD2-2 locus of P. moriformis strain S8588 by homologous recombination using previously described transformation methods (biolistics). The constructs that have been expressed in S8588 are listed in Table 11. S8588 is a recombinant P. moriformis strain having a FATA knockout and expressing an exogenous SUC2 gene and an exogenous P. moriformis KASII gene in the FATA locus. FATA knockouts that express sucrose invertase and/or KASII are described in co-owned applications WO2012/106560, WO2013/158938, WO2015/051319 and their respective priority applications thereof, all of which are herein incorporated by reference.

TABLE-US-00011 TABLE 11 DNA lot# and plasmid ID of DNA constructs that expressing wild-type and mutant BnOTE genes SEQ DNA Solazyme ID Lot# Plasmid NO: Construct D5309 pSZ6315 57 FAD2-2::PmHXT1-ScarMEL1-PmPGK:PmSAD2-2 V3-CpSADtp-BnOTE-PmSAD2-1 utr::FAD2-2 D5310 pSZ6316 58 FAD2-2::PmHXT1-ScarMEL1-PmPGK:PmSAD2-2 V3-CpSADtp-BnOTE(D124A)-PmSAD2-1 utr::FAD2-2 D5311 pSZ6317 59 FAD2-2::PmHXT1-ScarMEL1-PmPGK:PmSAD2-2 V3-CpSADtp-BnOTE(D209A)-PmSAD2-1 utr::FAD2-2 D5312 pSZ6318 60 FAD2-2::PmHXT1-ScarMEL1-PmPGK:PmSAD2-2 V3-CpSADtp-BnOTE(D124A, D209A)-PmSAD2-1 utr::FAD2-2
Construct pSZ6315: FAD2-2::PmHXT1-ScarMEL1-PmPGK:PmSAD2-2 V3-CpSADtp-BnOTE-PmSAD2-1 utr::FAD2-2

[0109] The sequence of the pSZ6315 transforming DNA is provided in SEQ ID NO: 57. Relevant restriction sites in pSZ6315 are indicated in lowercase, bold and underlining and are 5-3 SgrAI, Kpn I, SnaBI, AvrII, SpeI, AscI, ClaI, Sac I, SbfI, respectively. SgrAI and SbfI sites delimit the 5 and 3 ends of the transforming DNA. Bold, lowercase sequences represent FAD2-2 genomic DNA that permit targeted integration at FAD2-2 locus via homologous recombination. Proceeding in the 5 to 3 direction, the P. moriformis HXT1 promoter driving the expression of the Saccharomyces carlsbergensis MEL1 gene is indicated by boxed text. The initiator ATG and terminator TGA for MEL1 gene are indicated by uppercase, bold italics while the coding region is indicated in lowercase italics. The P. moriformis PGK 3 UTR is indicated by lowercase underlined text followed by the P. moriformis SAD2-2 V3 promoter, indicated by boxed italics text. The Initiator ATG and terminator TGA codons of the wild-type BnOTE are indicated by uppercase, bold italics, while the remainder of the coding region is indicated by bold italics. The three-nucleotide codon corresponding to the target two amino acids, D124 and D209, are double underlined. The P. moriformis SAD2-1 3UTR is again indicated by lowercase underlined text followed by the FAD2-2 genomic region indicated by bold, lowercase text.

Construct pSZ6316: FAD2-2::PmHXT1-ScarMEL1-PmPGK:PmSAD2-2 V3-CpSADtp-BnOTE (D124A)-PmSAD2-1 utr::FAD2-2

[0110] The sequence of the pSZ6316 transforming DNA is same as pSZ6315 except the D124A point mutation, the BnOTE D124A DNA sequence is provided in SEQ ID NO: 58.

Construct pSZ6317: FAD2-2::PmHXT1-ScarMEL1-PmPGK:PmSAD2-2 V3-CpSADtp-BnOTE (D209A)-PmSAD2-1 utr::FAD2-2

[0111] The sequence of the pSZ6317 transforming DNA is same as pSZ6315 except the D209A point mutation, the BnOTE D209A DNA sequence is provided in SEQ ID NO: 59.

Construct pSZ6318: FAD2-2::PmHXT1-ScarMEL1-PmPGK:PmSAD2-2 V3-CpSADtp-BnOTE (D124A, D209A)-PmSAD2-1 utr::FAD2-2

[0112] The sequence of the pSZ6318 transforming DNA is same as pSZ6315 except two point mutations, D124A and D209A, the BnOTE (D124A, D209A) DNA sequence is provided in SEQ ID NO:60.

Results

[0113] The DNA constructs containing the wild-type and mutant BnOTE genes were transformed into the low palmitate parental strain S8588, primary transformants were clonally purified and grown under standard lipid production conditions at pH5.0. The resulting profiles from representative clones arising from transformations with pSZ6315, pSZ6316, pSZ6317, and pSZ6318 into S8588 are shown in Table 12. The parental strain S8588 produces 5.4% C18:0, when transformed with the DNA cassette expressing wild-type BnOTE, the transgenic lines produce approximately 11% C18:0. The BnOTE mutant (D124A) increased the amount of C18:0 by at least 2 fold compared to the wild-type protein. In contrast, the BnOTE D209A mutation appears to have no impact on the enzyme activity/specificity of the BnOTE thioesterase. Finally, expression of the BnOTE (D124A, D209A) resulted in very similar fatty acid profile to what we observed in the transformants from S8588 expressing BnOTE (S124A), again indicating that D209A has no significant impact on the enzyme activity.

TABLE-US-00012 TABLE 12 Fatty acid profiles in S8588 and derivative transgenic lines transformed with wild-type and mutant BnOTE genes Fatty Acid Area % Transforming DNA Sample ID C16:0 C18:0 C18:1 C18:2 pH5; S8588 (parental strain) 3.00 5.43 81.75 6.47 D5309, pSZ6315, pH5; S8588, D5309-6; 3.86 11.68 76.51 5.06 wild-type BnOTE pH5; S8588, D5309-2; 3.50 11.00 77.80 4.95 pH5; S8588, D5309-9; 3.51 10.72 78.03 5.00 pH5; S8588, D5309-10; 3.55 10.69 78.06 4.96 pH5; S8588, D5309-11; 3.61 10.69 78.05 4.95 D5310, pSZ6316, pH5; S8588, D5310-6; 4.27 31.55 55.31 5.30 BnOTE (D124A) pH5; S8588, D5310-1; 4.53 30.85 54.71 6.03 pH5; S8588, D5310-5; 5.21 20.75 65.43 5.02 pH5; S8588, D5310-10; 4.99 19.18 67.75 5.00 pH5; S8588, D5310-2; 4.90 18.92 68.17 4.98 D5311, pSZ6317, pH5; S8588, D5311-3; 3.50 11.90 76.95 4.98 BnOTE (D209A) pH5; S8588, D5311-4; 3.63 11.35 77.44 4.94 pH5; S8588, D5311-14; 3.47 11.23 77.68 4.98 pH5; S8588, D5311-10; 3.60 11.20 77.53 5.00 pH5; S8588, D5311-12; 3.53 11.12 77.59 5.09 D5312, pSZ6318, pH5; S8588, D5312-20; 4.79 37.97 47.74 6.01 BnOTE (D127A, pH5; S8588, D5312-40; 5.97 22.94 62.20 5.11 D212A) pH5; S8588, D5312-39; 6.07 22.75 62.24 5.17 pH5; S8588, D5312-16; 5.25 18.81 67.36 5.09 pH5; S8588, D5312-26; 4.93 18.70 68.37 4.96

[0114] It is understood that the examples and embodiments described herein are for illustrative purposes only and that various modifications or changes in light thereof will be suggested to persons skilled in the art and are to be included within the spirit and purview of this application and scope of the appended claims. All publications, patents, and patent applications cited herein are hereby incorporated by reference in their entirety for all purposes.

Informal Sequence Listing

[0115]

TABLE-US-00013 InformalSequenceListing CupheacrassifloraFATBaminoacidsequence (CcrasFATB1) SEQIDNO:1 MVAAAASSAFFPVPAPGTSTKPRKSGNWPSRLSPSSKPKSIPNGGFQVK ANASAHPKANGSAVNLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLTA ITIVFVAAEKQWTMLDRKSKRPDMLVDSVGLKSIVRDGLVSRQSFSIRS YEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGMCKNDLIW VLTKMQIMVNRYPTWGDTVEINTWFSQSGKIGMGSDWLISDCNTGEILI RATSVWAMMNQKTRRFSRLPYEVRQELTPHFVDSPHVIEDNDRKLHKFD VKTGDSIRKGLTPRWNDLDVNQHVSNVKYIGWILESMPIEVLETQELCS LTVEYRRECGMDSKLESVTAMDPSEEDGVRSQYNHLLRLEDGTDVVKGR TEWRPKNAGTNGAISTGKTSNGNSVS CupheakoehneanaFATBaminoacidsequence (CkoeFATB3) SEQIDNO:2 MVTAAASSAFFPVPAPGTSPKPGKSWPSSLSPSFKPKSIPNAGFQVKAN ASAHPKANGSAVNLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLTAIT TVFVAAEKQWTMRDRKSKRPDMLVDSVGSKSIVLDGLVSRQIFSIRSYE IGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGMCKNDLIWVL TKMQIMVNRYPTWGDTVEINTWFSHSGKIGMASDWLITDCNTGEILIRA TSVWAMMNQKTRRFSRLPYEVRQELTPHYVDSPHVIEDNDRKLHKFDVK TGDSIRKGLTPKWNDLDVNQHVNNVKYIGWILESMPIEVLETQELCSLT VEYRRECGMDSVLESVTAMDPSEDGGLSQYKHLLRLEDGTDIVKGRTEW RPKNAGTNGAISTAKPSNGNSVS CuphealeptopodaFATBaminoacidsequence (C1eptFATB1) SEQIDNO:3 MVGAAASSAFFPAPAPGTSPKPGKSGNWPSSLSPSLKPKSIPNGGFQVK ANASAHPKANGAAVNLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLTA ITTVFVAAEKQWTMLDRKSKRPDMLVDSVGLKNIVRDGLVSRQSFSIRS YEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGMCKNDLIW VLTKMQILVNRYPAWGDTVEINTWFSQSGKIGMGSDWLISDCNTGEILI RATSVWAMMNQKTRRFSRLPYEVRQELTPHFVDSPHVIEDNDRKLHKFD VKTGDSIRKGLTPRWNDLDVNQHVSNVKYIGWILESMPIEVLETQELCS LTVEYRRECGMDSVLESVTARDPSEDGGRSQYNHLLRLEDGTDVVKGRT EWRSKNAGTNGATSTAKTSNGNSVS CupheaangustifoliaFATBaminoacidsequence (CangFATB1) SEQIDNO:4 MVAAAASSAFFPVPAPGTSLKPGKSGNWPSSLSPSFKPKTIPSGGLQVK ANASAHPKANGSAVNLKSGSLDTQEDTSSSPPPRAFLNQLPDWSMLLTA ITTVFVAAEKQWTMLDRKSKRPEMLVDSVGLKSSVRDGLVSRQSFSIRS YEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGMCKNDLIW VLTKMQIMVNRYPTWGDTVEVNTWFSQSGKIGMASDWLISDCNTGEILI RATSVWAMMNQKTRRFSRLPYEVRQELTPHYVDSPHVIEDNDRKLHKFD VKTGDSIRKGLTPRWNDLDVNQHVSNVKYIGWILESMPIEVLETQELCS LTVEYRRECGMDSVLESVTAMDPSEDGGVSQYKHLLRLEDGTDIVKGRT EWRPKNAGTNGATSKAKTSNGNSVS CupheallaveaFATB1aminoacidsequence (CllaFATB1) SEQIDNO:5 MVAAAASSAFFPAPAPGSSPKPGKPGNWPSSLSPSFKPKSIPNGRFQVK ANASAHPKANGSAVNLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLSA ITTVFVAAEKQWTMLDRKSKRPDMLVDSVGLKNIVRDGLVSRQSFSIRS YEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGMCKNDLIW VLTKMQIMVNRYPAWGDTVEINTWFSQSGKIGMGSDWLISDCNTGEILI RATSVWAMMNQKTRRFSRLPYEVRQELTPHFVDSPHVIEDNDRKLHKFD VKTGDSIRKGLTPRWNDLDVNQHVSNVKYIGWILESMPIEVLETQELCS LTVEYRRECGMDSVLESVTAIDPSEDGGRSQYNHLLRLDDGTDVVKGRT EWRPKNAGTNGAISTGKTSNGNSVS CuphealophostomaFATB1aminoacidsequence (ClopFATB1) SEQIDNO:6 MVAAAASSAFFPVPAPGTSLKPWKSGNWPSSLSPSFKPKTIPSGGFQVK ANASAQPKANGSAVNLKSGSLNTQEDTTSSPPPRAFLNQLPDWSMLLTA ITTVFVAAEKQWTMLDRKSKRPEKLVDSVGLKSSVRDGLVSRQSFSIRS YEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGMCKNDLIW VLTKMQIMVNRYPTWGDTVEINTWFSQSGKIGMASDWLISDCNTGEILI RATSVWAMMNQKTRRFSRLPYEVRQELTPHYVDSPHVIEDNDRKLHKFD VKTGDSIRKGLTPRWNDLDVNQHVSNVKYIGWILESMPIEVLETQELCS LTVEYRRECGMDSVLESVTAMDPSEDEGRSQYKHLLRLEDGTDIVKGRT EWRPKNAGTNGAISTAKNSNGNSVS SassafrasalbidumFATB1aminoacidsequence (SalFATB1) SEQIDNO:7 MATTSLASAFCSMKAVMLARDGRGMKPRSSDLQLRAGNAQTPLKMINGT KFSYTESLKRLPDWSMLFAVITTIFSVAEKQWTNLEWKPKPKPRLPQLL DDHFGLHGLVERRTFAIRSYEVGPDRSTSIVAVMNHLQEATLNHAKSVG ILGDGFGTTLEMSKRDLAWVVRRTHVAVERYPAWGDTVEVECWIGASGN NGMRRDFLVRDCKTGEILTRCTSLSVMMNTRTRRLSKIPEEVRGEIGPL FIDNVAVKDEEIKKLQKLNDSSADYIQGGLTPRWNDLDVNQHVNNIKYV GWILETVPDSIFESHHISSITLEYRRECTRDSVLQSLTTVSGGSLEAGL VCDHLLQLEGGSEVLRARTEWRPKLTDSFRGIIVIPAEPSV SassafrasalbidumFATB2aminoacidsequence (SalFATB2) SEQIDNO:8 MATTSLASAFCSMKAVMLARDGRGMKPRSSDLQLRAGNAQTPLKMINGT KESYTESLKRLPDWSMLFAVITTIFSVAEKQWTNLEWKPKPKPRLPQLL DDHFGLHGLVFRRTFAIRSYEVGPDRSTSIVAVMNHLQEATLNHAKSVG ILGDGFGTTLEMSKRDLAWVVRRTHVAVERYPAWGDTVEVEAWVGASGN IGMRRDFLVRDCKTGHILARCTSVSVMMNARTRRLSKIPQEVRAEIDPL FIEKVAVKEGEIKKLQKFNDSTADYIQGGWTPRWNDLDVNQHVNNIKYI GWIFKSVPDSISENHYLSSITLEYRRECTRGSALQSLTTVCGDSSEAGI ICEHLLQLEDGPEVLRARTEWRPKLTDSFRGIIVIPAEPSV LinderabenzoinFATB1aminoacidsequence (LbeFATB1) SEQIDNO:9 MVATSLASAFCSMKAVMLADDGRGMKPRSSDLQLRAGNAQTSLKMIDGT KFSYTESLKRLPDWSKLLTVITTIFSAAEKQWTNLERKPKPPHLLDDRF GLHGLVFRRTFAIRSYEVGPDRSASILAVLNHLQEATLNHAESVGILGD RFGETLEMSKRDLMWVVRRTYVAVERYPAWGDTVEIESWIGASGNNGMR REFLVRDFKTGEILTRCTSLSVMMNTRTRRLSKIPEEVRGEIGPVFIDN VAVKDEEIKKLQKLNDSTADYIQGGLIPRWNDLDLNQHVNNIKYVSWIL ETVPDSILESYHMSSITLEYRRECTRDSVLQSLTTVSGGSSEAGLVCEH SLLLEGGSEVLRARTEWRPKLTDSFRGISVIPAEQSV CupheacrassifloraFATBaminoacidsequence (CcrasFATB1),withouttargetingpeptide SEQIDNO:10 MVAAAASSAFFPVPAPGTSTKPRKSGNWPSRLSPSSKPKSIPNGGFQVK ANASAHPKANGSAVNLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLTA ITTVFVAAEKQWTMLDRKSKRPDMLVDSVGLKSIVRDGLVSRQSFSIRS YEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGMCKNDLIW VLTKMQIMVNRYPTWGDTVEINTWFSQSGKIGMGSDWLISDCNTGEILI RATSVWAMMNQKTRRFSRLPYEVRQELTPHFVDSPHVIEDNDRKLHKFD VKTGDSIRKGLTPRWNDLDVNQHVSNVKYIGWILESMPIEVLETQELCS LTVEYRRECGMDSKLESVTAMDPSEEDGVRSQYNHLLRLEDGTDVVKGR TEWRPKNAGTNGAISTGKTSNGNSVS CupheakoehneanaFATBaminoacidsequence (CkoeFATB3),withouttargetingpeptide SEQIDNO:11 MVTAAASSAFFPVPAPGTSPKPGKSWPSSLSPSFKPKSIPNAGFQVKAN ASAHPKANGSAVNLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLTAIT TVFVAAEKQWTMRDRKSKRPDMLVDSVGSKSIVLDGLVSRQIFSIRSYE IGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGMCKNDLIWVL TKMQIMVNRYPTWGDTVEINTWFSHSGKIGMASDWLITDCNTGEILIRA TSVWAMMNQKTRRFSRLPYEVRQELTPHYVDSPHVIEDNDRKLHKFDVK TGDSIRKGLTPKWNDLDVNQHVNNVKYIGWILESMPIEVLETQELCSLT VEYRRECGMDSVLESVTAMDPSEDGGLSQYKHLLRLEDGTDIVKGRTEW RPKNAGTNGAISTAKPSNGNSVS CuphealeptopodaFATBaminoacidsequence (CleptFATB1),withouttargetingpeptide SEQIDNO:12 MVGAAASSAFFPAPAPGTSPKPGKSGNWPSSLSPSLKPKSIPNGGFQVK ANASAHPKANGAAVNLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLTA ITTVFVAAEKQWTMLDRKSKRPDMLVDSVGLKNIVRDGLVSRQSFSIRS YEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGMCKNDLIW VLTKMQILVNRYPAWGDTVEINTWFSQSGKIGMGSDWLISDCNTGEILI RATSVWAMMNQKTRRFSRLPYEVRQELTPHFVDSPHVIEDNDRKLHKFD VKTGDSIRKGLTPRWNDLDVNQHVSNVKYIGWILESMPIEVLETQELCS LTVEYRRECGMDSVLESVTARDPSEDGGRSQYNHLLRLEDGTDVVKGRT EWRSKNAGTNGATSTAKTSNGNSVS CupheaangustifoliaFATBaminoacidsequence (CangFATB1),withouttargetingpeptide SEQIDNO:13 MVAAAASSAFFPVPAPGTSLKPGKSGNWPSSLSPSFKPKTIPSGGLQVK ANASAHPKANGSAVNLKSGSLDTQEDTSSSPPPRAFLNQLPDWSMLLTA ITTVFVAAEKQWTMLDRKSKRPEMLVDSVGLKSSVRDGLVSRQSFSIRS YEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGMCKNDLIW VLTKMQIMVNRYPTWGDTVEVNTWFSQSGKIGMASDWLISDCNTGEILI RATSVWAMMNQKTRRFSRLPYEVRQELTPHYVDSPHVIEDNDRKLHKFD VKTGDSIRKGLTPRWNDLDVNQHVSNVKYIGWILESMPIEVLETQELCS LTVEYRRECGMDSVLESVTAMDPSEDGGVSQYKHLLRLEDGTDIVKGRT EWRPKNAGTNGATSKAKTSNGNSVS CupheallaveaFATB1aminoacidsequence (CllaFATB1),withouttargetingpeptide SEQIDNO:14 MVAAAASSAFFPAPAPGSSPKPGKPGNWPSSLSPSFKPKSIPNGRFQVK ANASAHPKANGSAVNLKSGSLNIQEDTSSSPPPRAFLNQLPDWSMLLSA ITTVFVAAEKQWTMLDRKSKRPDMLVDSVGLKNIVRDGLVSRQSFSIRS YEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGMCKNDLIW VLTKMQIMVNRYPAWGDTVEINTWFSQSGKIGMGSDWLISDCNTGEILI RATSVWAMMNQKTRRFSRLPYEVRQELTPHFVDSPHVIEDNDRKLHKFD VKTGDSIRKGLTPRWNDLDVNQHVSNVKYIGWILESMPIEVLETQELCS LTVEYRRECGMDSVLESVTAIDPSEDGGRSQYNHLLRLDDGTDVVKGRT EWRPKNAGTNGAISTGKTSNGNSVS CuphealophostomaFATB1aminoacidsequence (ClopFATB1),withouttargetingpeptide SEQIDNO:15 MVAAAASSAFFPVPAPGTSLKPWKSGNWPSSLSPSFKPKTIPSGGFQVK ANASAQPKANGSAVNLKSGSLNTQEDTTSSPPPRAFLNQLPDWSMLLTA ITTVFVAAEKQWTMLDRKSKRPEKLVDSVGLKSSVRDGLVSRQSFSIRS YEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGMCKNDLIW VLTKMQIMVNRYPTWGDTVEINTWFSQSGKIGMASDWLISDCNTGEILI RATSVWAMMNQKTRRFSRLPYEVRQELTPHYVDSPHVIEDNDRKLHKFD VKTGDSIRKGLTPRWNDLDVNQHVSNVKYIGWILESMPIEVLETQELCS LTVEYRRECGMDSVLESVTAMDPSEDEGRSQYKHLLRLEDGTDIVKGRT EWRPKNAGTNGAISTAKNSNGNSVS SassafrasalbidumFATB1aminoacidsequence (SalFATB1),withouttargetingpeptide SEQIDNO:16 MATTSLASAFCSMKAVMLARDGRGMKPRSSDLQLRAGNAQTPLKMINGT KFSYTESLKRLPDWSMLFAVITTIFSVAEKQWTNLEWKPKPKPRLPQLL DDHFGLHGLVERRTFAIRSYEVGPDRSTSIVAVMNHLQEATLNHAKSVG ILGDGFGTTLEMSKRDLAWVVRRTHVAVERYPAWGDTVEVECWIGASGN NGMRRDFLVRDCKTGEILTRCTSLSVMMNTRTRRLSKIPEEVRGEIGPL FIDNVAVKDEEIKKLQKLNDSSADYIQGGLTPRWNDLDVNQHVNNIKYV GWILETVPDSIFESHHISSITLEYRRECTRDSVLQSLTTVSGGSLEAGL VCDHLLQLEGGSEVLRARTEWRPKLTDSFRGIIVIPAEPSV SassafrasalbidumFATB2aminoacidsequence (SalFATB2),withouttargetingpeptide SEQIDNO:17 MATTSLASAFCSMKAVMLARDGRGMKPRSSDLQLRAGNAQTPLKMINGT KFSYTESLKRLPDWSMLFAVITTIFSVAEKQWTNLEWKPKPKPRLPQLL DDHFGLHGLVFRRTFAIRSYEVGPDRSTSIVAVMNHLQEATLNHAKSVG ILGDGFGTTLEMSKRDLAWVVRRTHVAVERYPAWGDTVEVEAWVGASGN IGMRRDFLVRDCKTGHILARCTSVSVMMNARTRRLSKIPQEVRAEIDPL FIEKVAVKEGEIKKLQKFNDSTADYIQGGWTPRWNDLDVNQHVNNIKYI GWIFKSVPDSISENHYLSSITLEYRRECTRGSALQSLTTVCGDSSEAGI ICEHLLQLEDGPEVLRARTEWRPKLTDSFRGIIVIPAEPSV LinderabenzoinFATB1aminoacidsequence (LbeFATB1),withouttargetingpeptide SEQIDNO:18 MVATSLASAFCSMKAVMLADDGRGMKPRSSDLQLRAGNAQTSLKMIDGI KESYTESLKRLPDWSKLLTVITTIFSAAEKQWTNLERKPKPPHLLDDRF GLHGLVFRRTFAIRSYEVGPDRSASILAVLNHLQEATLNHAESVGILGD RFGETLEMSKRDLMWVVRRTYVAVERYPAWGDTVEIESWIGASGNNGMR REFLVRDFKTGEILTRCTSLSVMMNTRTRRLSKIPEEVRGEIGPVFIDN VAVKDEEIKKLQKLNDSTADYIQGGLIPRWNDLDLNQHVNNIKYVSWIL ETVPDSILESYHMSSITLEYRRECTRDSVLQSLTTVSGGSSEAGLVCEH SLLLEGGSEVLRARTEWRPKLTDSFRGISVIPAEQSV CupheacrassifloraFATBnativeCDSnucleicacid sequence(CcrasFATB1) SEQIDNO:19 ATGGTGGCTGCTGCAGCAAGTTCTGCATTCTTCCCTGTTCCTGCCCCAG GAACCTCCACTAAACCCAGGAAGTCCGGCAATTGGCCATCGAGATTGAG CCCTTCCTCCAAGCCCAAGTCAATCCCCAATGGCGGATTTCAGGTTAAG GCAAATGCCAGTGCCCATCCTAAGGCTAACGGTTCTGCAGTAAATCTAA AGTCTGGCAGCCTCAACACTCAGGAGGACACTTCGTCGTCCCCTCCTCC TCGGGCTTTCCTTAACCAGTTGCCTGATTGGAGTATGCTTCTGACTGCA ATCACGACCGTTTTCGTGGCGGCAGAGAAGCAGTGGACAATGCTTGATC GGAAATCTAAGAGGCCTGACATGCTCGTGGACTCGGTTGGGTTGAAGAG TATTGTTCGGGATGGGCTCGTGTCCAGACAAAGTTTTTCGATCAGGTCT TATGAAATAGGCGCTGATCGAACAGCCTCTATAGAGACGCTGATGAACC ACTTGCAGGAAACATCTATTAATCATTGTAAGAGTTTGGGCCTTCTCAA TGACGGCTTTGGTCGGACTCCTGGGATGTGTAAAAACGACCTCATTTGG GTGCTTACAAAAATGCAGATCATGGTGAATCGCTACCCAACTTGGGGCG ATACTGTTGAGATCAATACCTGGTTCTCCCAGTCGGGGAAAATCGGTAT GGGTAGCGATTGGCTAATAAGTGATTGCAATACAGGAGAAATTCTTATA AGGGCAACGAGCGTGTGGGCCATGATGAATCAAAAGACGAGAAGATTCT CAAGACTTCCATACGAGGTTCGCCAGGAGTTAACGCCTCATTTTGTGGA CTCTCCTCATGTCATTGAAGACAATGATCGGAAATTGCATAAGTTTGAT GTGAAGACTGGCGATTCTATTCGCAAGGGTCTAACTCCGAGGTGGAATG ATTTGGATGTCAATCAGCACGTAAGCAACGTGAAGTACATTGGGTGGAT TCTCGAGAGTATGCCAATAGAAGTTCTGGAGACCCAGGAGCTATGCTCT CTGACAGTTGAATATAGGCGGGAATGCGGAATGGACAGTAAGCTGGAGT CCGTGACTGCTATGGATCCCTCAGAAGAAGATGGAGTCCGGTCTCAGTA CAATCACCTTCTGCGGCTTGAGGATGGGACTGATGTCGTGAAGGGCAGA ACTGAGTGGCGACCGAAGAATGCAGGAACTAACGGGGCGATATCAACAG GAAAGACTTCAAATGGAAACTCGGTTTCTTAG CupheakoehneanaFATBFATBnativeCDSnucleic acidsequence(CkoeFATB3) SEQIDNO:20 ATGGTCACTGCTGCAGCAAGTTCTGCATTCTTCCCTGTTCCAGCCCCGG GAACCTCCCCTAAACCCGGGAAGTCCTGGCCATCGAGCTTGAGCCCTTC CTTCAAGCCCAAGTCAATCCCCAATGCCGGATTTCAGGTTAAGGCAAAT GCCAGTGCCCATCCTAAGGCTAACGGTTCTGCAGTAAATCTAAAGTCTG GCAGCCTCAACACTCAGGAGGACACTTCGTCGTCCCCTCCTCCTCGGGC TTTCCTTAACCAGTTGCCTGATTGGAGTATGCTTCTGACTGCAATCACG ACCGTCTTCGTGGCGGCAGAGAAGCAGTGGACTATGCGTGATCGGAAAT CTAAGAGGCCTGACATGCTCGTGGACTCGGTTGGATCGAAGAGTATTGT TCTGGATGGGCTCGTGTCCAGACAGATTTTTTCGATTAGATCTTATGAA ATAGGCGCTGATCGAACAGCCTCTATAGAGACGCTGATGAACCACTTGC AGGAAACATCTATCAATCATTGTAAGAGTTTGGGTCTTCTCAATGACGG CTTTGGTCGTACTCCTGGGATGTGTAAAAACGACCTCATTTGGGTGCTT ACAAAAATGCAGATCATGGTGAATCGCTACCCAACTTGGGGCGATACTG TTGAGATCAATACCTGGTTCTCCCATTCGGGGAAAATCGGTATGGCTAG CGATTGGCTAATAACTGATTGCAACACAGGAGAAATTCTTATAAGAGCA ACGAGCGTGTGGGCCATGATGAATCAAAAGACGAGAAGATTCTCAAGAC TTCCATACGAGGTTCGCCAGGAGTTAACGCCTCATTATGTGGACTCTCC TCATGTCATTGAAGATAATGATCGGAAATTGCATAAGTTTGATGTGAAG ACTGGTGATTCCATTCGTAAGGGTCTAACTCCGAAGTGGAATGACTTGG ATGTCAATCAGCACGTCAACAACGTGAAGTACATCGGGTGGATTCTCGA GAGTATGCCAATAGAAGTTTTGGAGACTCAGGAGCTATGCTCTCTCACC GTTGAATATAGGCGGGAATGCGGAATGGACAGTGTGCTGGAGTCCGTGA CTGCTATGGATCCCTCAGAAGATGGAGGCCTATCTCAGTACAAGCACCT TCTGCGGCTTGAGGATGGGACTGACATCGTGAAGGGCAGAACTGAGTGG CGACCGAAGAATGCAGGAACTAACGGGGCGATATCAACAGCAAAGCCTT CAAATGGAAACTCGGTCTCTTAG CuphealeptopodaFATBnativeCDSnucleicacid sequence(CleptFATB1) SEQIDNO:21 ATGGTGGGTGCTGCAGCAAGTTCTGCATTCTTCCCTGCTCCAGCCCCGG GAACCTCCCCTAAACCCGGGAAGTCCGGCAATTGGCCATCAAGCTTGAG CCCTTCCTTAAAGCCCAAGTCAATCCCCAATGGCGGATTTCAGGTTAAG GCAAATGCCAGTGCCCATCCTAAGGCTAACGGTGCTGCAGTAAATCTAA AGTCTGGCAGCCTCAACACTCAGGAGGACACTTCGTCGTCCCCTCCTCC TCGGGCTTTCCTTAACCAGTTGCCTGATTGGAGTATGCTTCTGACTGCA ATCACGACCGTCTTCGTGGCGGCAGAGAAGCAGTGGACTATGCTTGATC GGAAATCTAAGAGGCCTGACATGCTCGTGGACTCGGTTGGGTTGAAGAA TATTGTTCGGGATGGGCTCGTGTCCAGACAGAGTTTTTCGATCAGGTCT TATGAAATAGGCGCTGATCGAACAGCCTCTATAGAGACGCTGATGAACC ACTTGCAGGAAACATCTATCAATCATTGTAAGAGTTTGGGTCTTCTCAA TGACGGCTTTGGTCGTACTCCTGGGATGTGTAAAAACGACCTCATTTGG GTGCTTACAAAAATGCAGATCCTGGTGAATCGCTACCCAGCTTGGGGAG ATACTGTTGAGATCAATACCTGGTTCTCTCAGTCGGGGAAAATCGGCAT GGGTAGTGATTGGCTAATAAGTGATTGCAACACAGGAGAAATTCTTATA AGAGCAACGAGCGTGTGGGCAATGATGAATCAAAAGACGAGAAGATTCT CAAGACTTCCATACGAGGTTCGCCAGGAGTTAACGCCTCATTTTGTAGA CTCACCTCATGTCATTGAAGACAATGATCGGAAATTGCATAAGTTTGAT GTGAAGACTGGTGATTCTATTCGCAAGGGTCTAACTCCGAGGTGGAATG ACTTGGATGTCAATCAACACGTAAGCAACGTGAAGTACATTGGGTGGAT TCTCGAGAGTATGCCAATAGAAGTTTTGGAGACTCAGGAGCTATGCTCT CTCACCGTTGAATATAGGCGGGAATGCGGAATGGACAGTGTGCTGGAGT CCGTGACTGCTAGGGATCCCTCAGAAGATGGAGGCCGGTCTCAGTACAA TCACCTTCTGCGGCTTGAGGATGGGACTGATGTCGTGAAGGGCAGAACT GAGTGGCGATCGAAGAATGCAGGAACTAACGGGGCGACATCAACAGCAA AGACTTCAAATGGAAACTCGGTCTCTTAG CupheaangustifoliaFATBnativeCDSnucleicacid sequence(CangFATB1) SEQIDNO:22 ATGGTGGCTGCTGCAGCAAGTTCTGCATTCTTCCCTGTTCCAGCCCCGG GAACATCCCTTAAACCCGGGAAGTCCGGCAATTGGCCATCGAGCTTGAG CCCTTCCTTCAAGCCCAAGACAATCCCCAGTGGCGGACTTCAGGTTAAG GCAAATGCCAGTGCCCATCCTAAGGCTAACGGTTCTGCAGTAAATCTAA AGTCTGGCAGCCTCGACACTCAGGAGGACACTTCGTCGTCCCCTCCTCC TCGGGCTTTCCTTAACCAGTTGCCTGATTGGAGTATGCTTCTGACTGCA ATCACGACCGTCTTCGTGGCGGCAGAGAAGCAGTGGACTATGCTTGATA GGAAATCTAAGAGGCCTGAAATGCTCGTGGACTCGGTTGGGTTGAAGAG TAGTGTTCGGGATGGGCTCGTGTCCAGACAGAGTTTTTCGATTAGGTCT TATGAAATAGGCGCTGATCGAACAGCCTCTATAGAGACGCTGATGAACC ACTTGCAGGAAACATCTATCAATCATTGTAAGAGTTTGGGTCTTCTCAA CGATGGCTTTGGTCGTACTCCTGGGATGTGTAAAAACGACCTCATTTGG GTGCTTACAAAAATGCAGATCATGGTGAATCGCTACCCAACTTGGGGCG ATACTGTTGAGGTCAATACCTGGTTCTCCCAGTCGGGGAAAATCGGTAT GGCTAGCGATTGGCTAATCAGTGATTGCAACACAGGAGAAATTCTTATA AGAGCAACAAGCGTGTGGGCCATGATGAATCAAAAGACGAGAAGATTCT CAAGACTTCCATACGAGGTTCGCCAGGAGCTAACACCTCATTATGTGGA CTCTCCTCATGTCATTGAAGATAATGATCGGAAATTGCATAAGTTTGAT GTGAAGACTGGTGATTCCATTCGCAAGGGTCTAACTCCGAGGTGGAATG ACTTGGATGTCAATCAGCACGTAAGCAACGTGAAGTACATTGGGTGGAT TCTTGAGAGTATGCCAATAGAAGTTTTGGAGACCCAGGAGCTATGCTCT CTCACCGTTGAATATAGGCGGGAATGCGGAATGGACAGTGTGCTGGAGT CCGTGACTGCTATGGATCCCTCAGAAGATGGAGGCGTGTCTCAGTACAA GCACCTTCTGCGGCTTGAGGATGGGACTGATATCGTGAAGGGCAGAACT GAATGGCGACCGAAGAATGCAGGAACTAATGGGGCGACATCAAAAGCAA AGACTTCAAATGGAAACTCGGTCTCTTAG CupheallaveaFATB1nativeCDSnucleicacid sequence(CllaFATB1) SEQIDNO:23 ATGGTGGCTGCTGCAGCAAGTTCTGCATTCTTCCCTGCTCCAGCCCCGG GATCCTCACCTAAACCCGGGAAGCCCGGTAATTGGCCATCGAGCTTGAG CCCTTCCTTCAAGCCCAAGTCAATCCCCAATGGCCGATTTCAGGTTAAG GCAAATGCGAGTGCCCATCCTAAGGCTAACGGTTCTGCAGTAAATCTAA AGTCTGGCAGCCTCAACACTCAGGAGGACACTTCGTCGTCCCCTCCTCC TCGGGCTTTCCTTAACCAGTTGCCTGATTGGAGTATGCTTCTGTCTGCA ATCACGACTGTATTCGTGGCGGCAGAGAAGCAGTGGACTATGCTTGATC GGAAATCTAAGAGGCCTGACATGCTTGTGGACTCGGTTGGGTTGAAGAA TATTGTTCGGGATGGGCTCGTGTCCAGACAGAGTTTTTCGATTAGATCT TATGAAATAGGCGCTGATCGAACAGCTTCTATAGAGACACTGATGAACC ACTTGCAGGAAACATCTATCAATCATTGTAAGAGTTTGGGTCTTCTCAA TGACGGCTTTGGTCGTACTCCTGGGATGTGTAAAAACGACCTCATTTGG GTGCTTACAAAAATGCAGATCATGGTGAATCGCTACCCAGCTTGGGGCG ATACTGTTGAGATCAATACATGGTTCTCCCAGTCGGGGAAAATCGGTAT GGGTAGCGATTGGCTAATAAGTGATTGCAACACAGGAGAAATTCTTATA AGAGCAACGAGCGTGTGGGCCATGATGAATCAAAAGACGAGAAGATTCT CAAGACTTCCATATGAGGTTCGCCAGGAGTTAACGCCTCATTTTGTGGA CTCTCCTCATGTCATTGAAGACAATGATCGGAAATTGCATAAGTTCGAT GTGAAGACTGGTGATTCTATTCGCAAGGGTCTAACTCCGAGGTGGAATG ACTTGGATGTCAATCAACACGTAAGCAACGTGAAGTACATTGGGTGGAT TCTCGAGAGTATGCCAATAGAAGTTTTGGAGACCCAGGAACTATGCTCT CTCACAGTTGAATATAGGCGGGAATGCGGAATGGACAGTGTGCTGGAGT CCGTGACTGCTATAGATCCCTCAGAAGATGGAGGGCGGTCTCAGTACAA TCACCTTCTGCGGCTTGATGATGGGACTGATGTCGTGAAGGGCAGAACA GAGTGGCGACCGAAGAATGCAGGAACTAACGGGGCGATATCAACAGGAA AGACTTCAAATGGGAACTCGGTCTCCTAG CuphealophostomaFATB1nativeCDSnucleicacid sequence(ClopFATB1) SEQIDNO:24 ATGGTGGCTGCTGCAGCAAGTTCTGCATTCTTCCCTGTTCCAGCCCCGG GAACCTCCCTTAAACCCTGGAAGTCCGGAAATTGGCCATCGAGCTTGAG CCCTTCCTTCAAGCCCAAGACAATCCCCAGTGGCGGATTTCAGGTTAAG GCAAATGCCAGTGCCCAGCCTAAGGCTAACGGTTCTGCAGTAAATCTAA AGTCTGGCAGCCTCAACACTCAGGAGGACACAACGTCGTCGCCTCCTCC TCGGGCTTTCCTTAACCAGTTGCCTGATTGGAGTATGCTTCTGACTGCA ATCACGACCGTCTTCGTGGCGGCGGAGAAGCAGTGGACAATGCTTGATA GGAAATCTAAGAGGCCTGAAAAGCTCGTGGACTCGGTTGGGTTGAAGAG TAGTGTTCGGGATGGGCTCGTGTCCAGACAGAGTTTTTCGATTAGGTCT TATGAAATAGGCGCTGATCGAACAGCCTCTATAGAGACGTTGATGAACC ACTTGCAGGAAACATCTATCAATCATTGTAAGAGTTTGGGTCTTCTCAA CGACGGCTTTGGTCGTACTCCTGGGATGTGTAAAAACGACCTCATTTGG GTGCTTACGAAAATGCAGATCATGGTGAATCGCTACCCAACTTGGGGCG ATACTGTTGAGATCAATACCTGGTTCTCCCAGTCGGGGAAAATCGGTAT GGCTAGCGATTGGCTAATAAGTGATTGCAACACAGGAGAAATTCTTATA AGAGCAACGAGCGTGTGGGCCATGATGAATCAAAAGACGAGAAGGTTCT CAAGACTTCCATACGAGGTTCGCCAGGAGTTAACGCCTCATTATGTGGA CTCTCCTCATGTCATTGAAGACAATGATCGGAAATTGCATAAGTTTGAT GTGAAGACTGGTGATTCCATTCGCAAGGGTCTGACTCCGAGGTGGAATG ACTTGGATGTCAATCAGCACGTAAGCAACGTGAAGTACATTGGGTGGAT TCTGGAGAGTATGCCAATAGAAGTTTTGGAGACCCAGGAGCTATGCTCT CTCACCGTTGAATATAGGCGGGAATGCGGGATGGACAGTGTGCTGGAGT CCGTGACTGCTATGGATCCCTCAGAAGATGAAGGCCGGTCTCAGTACAA GCACCTTCTGCGGCTTGAGGATGGGACTGATATCGTGAAGGGCAGAACT GAGTGGCGACCGAAGAATGCAGGAACTAACGGGGCGATATCAACAGCAA AGAATTCAAATGGAAACTCGGTCTCTTAG SassafrasalbidumFATB1nativeCDSnucleicacid sequence(SalFATB1) SEQIDNO:25 ATGGCCACCACCTCTTTAGCTTCTGCTTTCTGCTCGATGAAAGCTGTAA TGTTGGCTCGTGATGGCAGGGGCATGAAACCCAGGAGCAGTGATTTGCA GCTGAGGGCGGGAAATGCACAAACCCCTTTGAAGATGATCAATGGGACC AAGTTCAGTTACACGGAGAGCTTGAAAAGGTTGCCTGACTGGAGCATGC TCTTTGCAGTGATCACAACCATCTTTTCGGTTGCTGAGAAGCAGTGGAC CAATCTAGAGTGGAAGCCGAAGCCGAAGCCGAGGCTACCCCAGTTGCTT GATGACCATTTTGGACTGCATGGGTTAGTTTTCAGGCGCACCTTTGCCA TCAGATCTTATGAGGTCGGACCTGACCGCTCCACATCTATAGTGGCTGT TATGAATCACTTGCAGGAGGCTACACTTAATCATGCGAAGAGTGTGGGA ATTCTAGGAGATGGATTCGGTACGACGCTAGAGATGAGTAAGAGAGATC TGGCGTGGGTTGTGAGACGCACGCATGTTGCTGTGGAACGGTACCCTGC TTGGGGTGATACTGTTGAAGTAGAGTGCTGGATTGGTGCATCTGGAAAT AATGGCATGCGCCGTGATTTCCTTGTCCGGGACTGCAAAACAGGCGAAA TTCTTACAAGATGTACCAGTCTTTCGGTGATGATGAATACAAGGACAAG GAGGTTGTCCAAAATCCCTGAAGAAGTTAGAGGGGAGATAGGGCCTCTA TTCATTGATAATGTGGCTGTCAAGGACGAGGAAATTAAGAAACTACAGA AGCTCAATGACAGCTCTGCAGATTACATCCAAGGAGGTTTGACTCCTCG ATGGAATGATTTGGATGTCAATCAGCATGTTAACAACATCAAATACGTT GGCTGGATTCTTGAGACTGTCCCAGACTCCATCTTTGAGAGTCATCATA TTTCCAGCATCACTCTTGAATACAGGAGAGAGTGCACCAGGGATAGCGT GCTGCAGTCCCTGACCACTGTCTCCGGTGGCTCGTTGGAGGCTGGGTTA GTGTGCGATCACTTGCTCCAGCTTGAAGGTGGGTCTGAGGTATTGAGGG CAAGAACAGAGTGGAGGCCTAAGCTTACCGATAGTTTCAGAGGGATTAT TGTGATACCCGCAGAACCGAGTGTGTAA SassafrasalbidumFATB2nativeCDSnucleicacid sequence(SalFATB2) SEQIDNO:26 ATGGCCACCACCTCTTTAGCTTCTGCTTTCTGCTCGATGAAAGCTGTAA TGTTGGCTCGTGATGGCAGGGGCATGAAACCCAGGAGCAGTGATTTGCA GCTGAGGGCGGGAAATGCACAAACCCCTTTGAAGATGATCAATGGGACC AAGTTCAGTTACACGGAGAGCTTGAAAAGGTTGCCTGACTGGAGCATGC TCTTTGCAGTGATCACAACCATCTTTTCGGTTGCTGAGAAGCAGTGGAC CAATCTAGAGTGGAAGCCGAAGCCGAAGCCGAGGCTACCCCAGTTGCTT GATGACCATTTTGGACTGCATGGGTTAGTTTTCAGGCGCACCTTTGCCA TCAGATCTTATGAGGTCGGACCTGACCGCTCCACATCTATAGTGGCTGT TATGAATCACTTGCAGGAGGCTACACTTAATCATGCGAAGAGTGTGGGA ATTCTAGGAGATGGATTCGGTACGACGCTAGAGATGAGTAAGAGAGATC TGGCGTGGGTTGTGAGACGCACGCATGTTGCTGTGGAACGGTACCCCGC TTGGGGCGATACTGTTGAAGTCGAGGCCTGGGTCGGTGCATCTGGAAAC ATTGGCATGCGCCGCGATTTTCTTGTCCGCGACTGCAAAACTGGCCACA TTCTTGCAAGATGTACCAGTGTTTCAGTGATGATGAATGCGAGGACACG GAGATTGTCCAAAATTCCCCAAGAAGTTAGAGCCGAGATTGACCCTCTT TTCATTGAAAAGGTTGCGGTCAAGGAAGGGGAAATTAAGAAATTACAGA AGTTCAATGATAGCACTGCAGATTACATTCAAGGGGGTTGGACTCCTCG ATGGAATGATTTGGATGTCAATCAGCACGTGAACAATATCAAATACATT GGCTGGATTTTTAAGAGCGTCCCAGACTCTATCTCTGAGAATCATTATC TTTCTAGCATCACTCTCGAATACAGGAGAGAGTGCACAAGGGGCAGCGC GCTGCAGTCCCTGACCACTGTTTGTGGTGACTCGTCGGAAGCTGGGATC ATATGTGAGCACCTACTCCAGCTTGAGGATGGGCCTGAGGTTTTGAGGG CAAGAACAGAGTGGAGGCCTAAGCTTACCGATAGTTTCAGAGGGATTAT TGTGATACCCGCAGAACCGAGTGTGTAA LinderabenzoinFATB1nativeCDSnucleicacid sequence(LbeFATB1) SEQIDNO:27 ATGGTCACTGCTGCAGCAAGTTCTGCATTCTTCCCTGTTCCAGCCCCGG GAACCTCCCCTAAACCCGGGAAGTCCTGGCCATCGAGCTTGAGCCCTTC CTTCAAGCCCAAGTCAATCCCCAATGCCGGATTTCAGGTTAAGGCAAAT GCCAGTGCCCATCCTAAGGCTAACGGTTCTGCAGTAAATCTAAAGTCTG GCAGCCTCAACACTCAGGAGGACACTTCGTCGTCCCCTCCTCCTCGGGC TTTCCTTAACCAGTTGCCTGATTGGAGTATGCTTCTGACTGCAATCACG ACCGTCTTCGTGGCGGCAGAGAAGCAGTGGACTATGCGTGATCGGAAAT CTAAGAGGCCTGACATGCTCGTGGACTCGGTTGGATCGAAGAGTATTGT TCTGGATGGGCTCGTGTCCAGACAGATTTTTTCGATTAGATCTTATGAA ATAGGCGCTGATCGAACAGCCTCTATAGAGACGCTGATGAACCACTTGC AGGAAACATCTATCAATCATTGTAAGAGTTTGGGTCTTCTCAATGACGG CTTTGGTCGTACTCCTGGGATGTGTAAAAACGACCTCATTTGGGTGCTT ACAAAAATGCAGATCATGGTGAATCGCTACCCAACTTGGGGCGATACTG TTGAGATCAATACCTGGTTCTCCCATTCGGGGAAAATCGGTATGGCTAG CGATTGGCTAATAACTGATTGCAACACAGGAGAAATTCTTATAAGAGCA ACGAGCGTGTGGGCCATGATGAATCAAAAGACGAGAAGATTCTCAAGAC TTCCATACGAGGTTCGCCAGGAGTTAACGCCTCATTATGTGGACTCTCC TCATGTCATTGAAGATAATGATCGGAAATTGCATAAGTTTGATGTGAAG ACTGGTGATTCCATTCGTAAGGGTCTAACTCCGAAGTGGAATGACTTGG ATGTCAATCAGCACGTCAACAACGTGAAGTACATCGGGTGGATTCTCGA GAGTATGCCAATAGAAGTTTTGGAGACTCAGGAGCTATGCTCTCTCACC GTTGAATATAGGCGGGAATGCGGAATGGACAGTGTGCTGGAGTCCGTGA CTGCTATGGATCCCTCAGAAGATGGAGGCCTATCTCAGTACAAGCACCT TCTGCGGCTTGAGGATGGGACTGACATCGTGAAGGGCAGAACTGAGTGG CGACCGAAGAATGCAGGAACTAACGGGGCGATATCAACAGCAAAGCCTT CAAATGGAAACTCGGTCTCTTAG CupheacrassifloraFATBnativeCDScodon optimizedacidsequence(CcrasFATB1) SEQIDNO:28 ATGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCGCCCCCG GCACCTCCACCAAGCCCCGCAAGTCCGGCAACTGGCCCTCCCGCCTGTC CCCCTCCTCCAAGCCCAAGTCCATCCCCAACGGCGGCTTCCAGGTGAAG GCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTCCGCCGTGAACCTGA AGTCCGGCTCCCTGAACACCCAGGAGGACACCTCCTCCTCCCCCCCCCC CCGCGCCTTCCTGAACCAGCTGCCCGACTGGTCCATGCTGCTGACCGCC ATCACCACCGTGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCTGGACC GCAAGTCCAAGCGCCCCGACATGCTGGTGGACTCCGTGGGCCTGAAGTC CATCGTGCGCGACGGCCTGGTGTCCCGCCAGTCCTTCTCCATCCGCTCC TACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGATGAACC ACCTGCAGGAGACCTCCATCAACCACTGCAAGTCCCTGGGCCTGCTGAA CGACGGCTTCGGCCGCACCCCCGGCATGTGCAAGAACGACCTGATCTGG GTGCTGACCAAGATGCAGATCATGGTGAACCGCTACCCCACCTGGGGCG ACACCGTGGAGATCAACACCTGGTTCTCCCAGTCCGGCAAGATCGGCAT GGGCTCCGACTGGCTGATCTCCGACTGCAACACCGGCGAGATCCTGATC CGCGCCACCTCCGTGTGGGCCATGATGAACCAGAAGACCCGCCGCTTCT CCCGCCTGCCCTACGAGGTGCGCCAGGAGCTGACCCCCCACTTCGTGGA CTCCCCCCACGTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGAC GTGAAGACCGGCGACTCCATCCGCAAGGGCCTGACCCCCCGCTGGAACG ACCTGGACGTGAACCAGCACGTGTCCAACGTGAAGTACATCGGCTGGAT CCTGGAGTCCATGCCCATCGAGGTGCTGGAGACCCAGGAGCTGTGCTCC CTGACCGTGGAGTACCGCCGCGAGTGCGGCATGGACTCCAAGCTGGAGT CCGTGACCGCCATGGACCCCTCCGAGGAGGACGGCGTGCGCTCCCAGTA CAACCACCTGCTGCGCCTGGAGGACGGCACCGACGTGGTGAAGGGCCGC ACCGAGTGGCGCCCCAAGAACGCCGGCACCAACGGCGCCATCTCCACCG GCAAGACCTCCAACGGCAACTCCGTGTCCTGA CupheakoehneanaFATBFATBcodonoptimizedCDS nucleicacidsequence(CkoeFATB3) SEQIDNO:29 ATGGTGACCGCCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCGCCCCCG GCACCTCCCCCAAGCCCGGCAAGTCCTGGCCCTCCTCCCTGTCCCCCTC CTTCAAGCCCAAGTCCATCCCCAACGCCGGCTTCCAGGTGAAGGCCAAC GCCTCCGCCCACCCCAAGGCCAACGGCTCCGCCGTGAACCTGAAGTCCG GCTCCCTGAACACCCAGGAGGACACCTCCTCCTCCCCCCCCCCCCGCGC CTTCCTGAACCAGCTGCCCGACTGGTCCATGCTGCTGACCGCCATCACC ACCGTGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCGCGACCGCAAGT CCAAGCGCCCCGACATGCTGGTGGACTCCGTGGGCTCCAAGTCCATCGT GCTGGACGGCCTGGTGTCCCGCCAGATCTTCTCCATCCGCTCCTACGAG ATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGATGAACCACCTGC AGGAGACCTCCATCAACCACTGCAAGTCCCTGGGCCTGCTGAACGACGG CTTCGGCCGCACCCCCGGCATGTGCAAGAACGACCTGATCTGGGTGCTG ACCAAGATGCAGATCATGGTGAACCGCTACCCCACCTGGGGCGACACCG TGGAGATCAACACCTGGTTCTCCCACTCCGGCAAGATCGGCATGGCCTC CGACTGGCTGATCACCGACTGCAACACCGGCGAGATCCTGATCCGCGCC ACCTCCGTGTGGGCCATGATGAACCAGAAGACCCGCCGCTTCTCCCGCC TGCCCTACGAGGTGCGCCAGGAGCTGACCCCCCACTACGTGGACTCCCC CCACGTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAG ACCGGCGACTCCATCCGCAAGGGCCTGACCCCCAAGTGGAACGACCTGG ACGTGAACCAGCACGTGAACAACGTGAAGTACATCGGCTGGATCCTGGA GTCCATGCCCATCGAGGTGCTGGAGACCCAGGAGCTGTGCTCCCTGACC GTGGAGTACCGCCGCGAGTGCGGCATGGACTCCGTGCTGGAGTCCGTGA CCGCCATGGACCCCTCCGAGGACGGCGGCCTGTCCCAGTACAAGCACCT GCTGCGCCTGGAGGACGGCACCGACATCGTGAAGGGCCGCACCGAGTGG CGCCCCAAGAACGCCGGCACCAACGGCGCCATCTCCACCGCCAAGCCCT CCAACGGCAACTCCGTGTCCTGA CuphealeptopodaFATBcodonoptimizedCDS nucleicacidsequence(CleptFATB1) SEQIDNO:30 ATGGTGGGCGCCGCCGCCTCCTCCGCCTTCTTCCCCGCCCCCGCCCCCG GCACCTCCCCCAAGCCCGGCAAGTCCGGCAACTGGCCCTCCTCCCTGTC CCCCTCCCTGAAGCCCAAGTCCATCCCCAACGGCGGCTTCCAGGTGAAG GCCAACGCCTCCGCCCACCCCAAGGCCAACGGCGCCGCCGTGAACCTGA AGTCCGGCTCCCTGAACACCCAGGAGGACACCTCCTCCTCCCCCCCCCC CCGCGCCTTCCTGAACCAGCTGCCCGACTGGTCCATGCTGCTGACCGCC ATCACCACCGTGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCTGGACC GCAAGTCCAAGCGCCCCGACATGCTGGTGGACTCCGTGGGCCTGAAGAA CATCGTGCGCGACGGCCTGGTGTCCCGCCAGTCCTTCTCCATCCGCTCC TACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGATGAACC ACCTGCAGGAGACCTCCATCAACCACTGCAAGTCCCTGGGCCTGCTGAA CGACGGCTTCGGCCGCACCCCCGGCATGTGCAAGAACGACCTGATCTGG GTGCTGACCAAGATGCAGATCCTGGTGAACCGCTACCCCGCCTGGGGCG ACACCGTGGAGATCAACACCTGGTTCTCCCAGTCCGGCAAGATCGGCAT GGGCTCCGACTGGCTGATCTCCGACTGCAACACCGGCGAGATCCTGATC CGCGCCACCTCCGTGTGGGCCATGATGAACCAGAAGACCCGCCGCTTCT CCCGCCTGCCCTACGAGGTGCGCCAGGAGCTGACCCCCCACTTCGTGGA CTCCCCCCACGTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGAC GTGAAGACCGGCGACTCCATCCGCAAGGGCCTGACCCCCCGCTGGAACG ACCTGGACGTGAACCAGCACGTGTCCAACGTGAAGTACATCGGCTGGAT CCTGGAGTCCATGCCCATCGAGGTGCTGGAGACCCAGGAGCTGTGCTCC CTGACCGTGGAGTACCGCCGCGAGTGCGGCATGGACTCCGTGCTGGAGT CCGTGACCGCCCGCGACCCCTCCGAGGACGGCGGCCGCTCCCAGTACAA CCACCTGCTGCGCCTGGAGGACGGCACCGACGTGGTGAAGGGCCGCACC GAGTGGCGCTCCAAGAACGCCGGCACCAACGGCGCCACCTCCACCGCCA AGACCTCCAACGGCAACTCCGTGTCCTGA CupheaangustifoliaFATBcodonoptimizedCDS nucleicacidsequence(CangFATB1) SEQIDNO:31 ATGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCGCCCCCG GCACCTCCCTGAAGCCCGGCAAGTCCGGCAACTGGCCCTCCTCCCTGTC CCCCTCCTTCAAGCCCAAGACCATCCCCTCCGGCGGCCTGCAGGTGAAG GCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTCCGCCGTGAACCTGA AGTCCGGCTCCCTGGACACCCAGGAGGACACCTCCTCCTCCCCCCCCCC CCGCGCCTTCCTGAACCAGCTGCCCGACTGGTCCATGCTGCTGACCGCC ATCACCACCGTGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCTGGACC GCAAGTCCAAGCGCCCCGAGATGCTGGTGGACTCCGTGGGCCTGAAGTC CTCCGTGCGCGACGGCCTGGTGTCCCGCCAGTCCTTCTCCATCCGCTCC TACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGATGAACC ACCTGCAGGAGACCTCCATCAACCACTGCAAGTCCCTGGGCCTGCTGAA CGACGGCTTCGGCCGCACCCCCGGCATGTGCAAGAACGACCTGATCTGG GTGCTGACCAAGATGCAGATCATGGTGAACCGCTACCCCACCTGGGGCG ACACCGTGGAGGTGAACACCTGGTTCTCCCAGTCCGGCAAGATCGGCAT GGCCTCCGACTGGCTGATCTCCGACTGCAACACCGGCGAGATCCTGATC CGCGCCACCTCCGTGTGGGCCATGATGAACCAGAAGACCCGCCGCTTCT CCCGCCTGCCCTACGAGGTGCGCCAGGAGCTGACCCCCCACTACGTGGA CTCCCCCCACGTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGAC GTGAAGACCGGCGACTCCATCCGCAAGGGCCTGACCCCCCGCTGGAACG ACCTGGACGTGAACCAGCACGTGTCCAACGTGAAGTACATCGGCTGGAT CCTGGAGTCCATGCCCATCGAGGTGCTGGAGACCCAGGAGCTGTGCTCC CTGACCGTGGAGTACCGCCGCGAGTGCGGCATGGACTCCGTGCTGGAGT CCGTGACCGCCATGGACCCCTCCGAGGACGGCGGCGTGTCCCAGTACAA GCACCTGCTGCGCCTGGAGGACGGCACCGACATCGTGAAGGGCCGCACC GAGTGGCGCCCCAAGAACGCCGGCACCAACGGCGCCACCTCCAAGGCCA AGACCTCCAACGGCAACTCCGTGTCCTGA CupheallaveaFATB1codonoptimizedCDSnucleic acidsequence(CllaFATB1) SEQIDNO:32 ATGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGCCCCCGCCCCCG GCTCCTCCCCCAAGCCCGGCAAGCCCGGCAACTGGCCCTCCTCCCTGTC CCCCTCCTTCAAGCCCAAGTCCATCCCCAACGGCCGCTTCCAGGTGAAG GCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTCCGCCGTGAACCTGA AGTCCGGCTCCCTGAACACCCAGGAGGACACCTCCTCCTCCCCCCCCCC CCGCGCCTTCCTGAACCAGCTGCCCGACTGGTCCATGCTGCTGTCCGCC ATCACCACCGTGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCTGGACC GCAAGTCCAAGCGCCCCGACATGCTGGTGGACTCCGTGGGCCTGAAGAA CATCGTGCGCGACGGCCTGGTGTCCCGCCAGTCCTTCTCCATCCGCTCC TACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGATGAACC ACCTGCAGGAGACCTCCATCAACCACTGCAAGTCCCTGGGCCTGCTGAA CGACGGCTTCGGCCGCACCCCCGGCATGTGCAAGAACGACCTGATCTGG GTGCTGACCAAGATGCAGATCATGGTGAACCGCTACCCCGCCTGGGGCG ACACCGTGGAGATCAACACCTGGTTCTCCCAGTCCGGCAAGATCGGCAT GGGCTCCGACTGGCTGATCTCCGACTGCAACACCGGCGAGATCCTGATC CGCGCCACCTCCGTGTGGGCCATGATGAACCAGAAGACCCGCCGCTTCT CCCGCCTGCCCTACGAGGTGCGCCAGGAGCTGACCCCCCACTTCGTGGA CTCCCCCCACGTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGAC GTGAAGACCGGCGACTCCATCCGCAAGGGCCTGACCCCCCGCTGGAACG ACCTGGACGTGAACCAGCACGTGTCCAACGTGAAGTACATCGGCTGGAT CCTGGAGTCCATGCCCATCGAGGTGCTGGAGACCCAGGAGCTGTGCTCC CTGACCGTGGAGTACCGCCGCGAGTGCGGCATGGACTCCGTGCTGGAGT CCGTGACCGCCATCGACCCCTCCGAGGACGGCGGCCGCTCCCAGTACAA CCACCTGCTGCGCCTGGACGACGGCACCGACGTGGTGAAGGGCCGCACC GAGTGGCGCCCCAAGAACGCCGGCACCAACGGCGCCATCTCCACCGGCA AGACCTCCAACGGCAACTCCGTGTCCTGA CuphealophostomaFATB1codonoptimizedCDS nucleicacidsequence(ClopFATB1) SEQIDNO:33 ATGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCGCCCCCG GCACCTCCCTGAAGCCCTGGAAGTCCGGCAACTGGCCCTCCTCCCTGTC CCCCTCCTTCAAGCCCAAGACCATCCCCTCCGGCGGCTTCCAGGTGAAG GCCAACGCCTCCGCCCAGCCCAAGGCCAACGGCTCCGCCGTGAACCTGA AGTCCGGCTCCCTGAACACCCAGGAGGACACCACCTCCTCCCCCCCCCC CCGCGCCTTCCTGAACCAGCTGCCCGACTGGTCCATGCTGCTGACCGCC ATCACCACCGTGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCTGGACC GCAAGTCCAAGCGCCCCGAGAAGCTGGTGGACTCCGTGGGCCTGAAGTC CTCCGTGCGCGACGGCCTGGTGTCCCGCCAGTCCTTCTCCATCCGCTCC TACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGATGAACC ACCTGCAGGAGACCTCCATCAACCACTGCAAGTCCCTGGGCCTGCTGAA CGACGGCTTCGGCCGCACCCCCGGCATGTGCAAGAACGACCTGATCTGG GTGCTGACCAAGATGCAGATCATGGTGAACCGCTACCCCACCTGGGGCG ACACCGTGGAGATCAACACCTGGTTCTCCCAGTCCGGCAAGATCGGCAT GGCCTCCGACTGGCTGATCTCCGACTGCAACACCGGCGAGATCCTGATC CGCGCCACCTCCGTGTGGGCCATGATGAACCAGAAGACCCGCCGCTTCT CCCGCCTGCCCTACGAGGTGCGCCAGGAGCTGACCCCCCACTACGTGGA CTCCCCCCACGTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGAC GTGAAGACCGGCGACTCCATCCGCAAGGGCCTGACCCCCCGCTGGAACG ACCTGGACGTGAACCAGCACGTGTCCAACGTGAAGTACATCGGCTGGAT CCTGGAGTCCATGCCCATCGAGGTGCTGGAGACCCAGGAGCTGTGCTCC CTGACCGTGGAGTACCGCCGCGAGTGCGGCATGGACTCCGTGCTGGAGT CCGTGACCGCCATGGACCCCTCCGAGGACGAGGGCCGCTCCCAGTACAA GCACCTGCTGCGCCTGGAGGACGGCACCGACATCGTGAAGGGCCGCACC GAGTGGCGCCCCAAGAACGCCGGCACCAACGGCGCCATCTCCACCGCCA AGAACTCCAACGGCAACTCCGTGTCCTGA SassafrasalbidumFATB1codonoptimizedCDS nucleicacidsequence(SalFATB1) SEQIDNO:34 ATGGCCACCACCTCCCTGGCCTCCGCCTTCTGCTCCATGAAGGCCGTGA TGCTGGCCCGCGACGGCCGCGGCATGAAGCCCCGCTCCTCCGACCTGCA GCTGCGCGCCGGCAACGCCCAGACCCCCCTGAAGATGATCAACGGCACC AAGTTCTCCTACACCGAGTCCCTGAAGCGCCTGCCCGACTGGTCCATGC TGTTCGCCGTGATCACCACCATCTTCTCCGTGGCCGAGAAGCAGTGGAC CAACCTGGAGTGGAAGCCCAAGCCCAAGCCCCGCCTGCCCCAGCTGCTG GACGACCACTTCGGCCTGCACGGCCTGGTGTTCCGCCGCACCTTCGCCA TCCGCTCCTACGAGGTGGGCCCCGACCGCTCCACCTCCATCGTGGCCGT GATGAACCACCTGCAGGAGGCCACCCTGAACCACGCCAAGTCCGTGGGC ATCCTGGGCGACGGCTTCGGCACCACCCTGGAGATGTCCAAGCGCGACC TGGCCTGGGTGGTGCGCCGCACCCACGTGGCCGTGGAGCGCTACCCCGC CTGGGGCGACACCGTGGAGGTGGAGTGCTGGATCGGCGCCTCCGGCAAC AACGGCATGCGCCGCGACTTCCTGGTGCGCGACTGCAAGACCGGCGAGA TCCTGACCCGCTGCACCTCCCTGTCCGTGATGATGAACACCCGCACCCG CCGCCTGTCCAAGATCCCCGAGGAGGTGCGCGGCGAGATCGGCCCCCTG TTCATCGACAACGTGGCCGTGAAGGACGAGGAGATCAAGAAGCTGCAGA AGCTGAACGACTCCTCCGCCGACTACATCCAGGGCGGCCTGACCCCCCG CTGGAACGACCTGGACGTGAACCAGCACGTGAACAACATCAAGTACGTG GGCTGGATCCTGGAGACCGTGCCCGACTCCATCTTCGAGTCCCACCACA TCTCCTCCATCACCCTGGAGTACCGCCGCGAGTGCACCCGCGACTCCGT GCTGCAGTCCCTGACCACCGTGTCCGGCGGCTCCCTGGAGGCCGGCCTG GTGTGCGACCACCTGCTGCAGCTGGAGGGCGGCTCCGAGGTGCTGCGCG CCCGCACCGAGTGGCGCCCCAAGCTGACCGACTCCTTCCGCGGCATCAT CGTGATCCCCGCCGAGCCCTCCGTGTGA SassafrasalbidumFATB2codonoptimizedCDS nucleicacidsequence(SalFATB2) SEQIDNO:35 ATGGCCACCACCTCCCTGGCCTCCGCCTTCTGCTCCATGAAGGCCGTGA TGCTGGCCCGCGACGGCCGCGGCATGAAGCCCCGCTCCTCCGACCTGCA GCTGCGCGCCGGCAACGCCCAGACCCCCCTGAAGATGATCAACGGCACC AAGTTCTCCTACACCGAGTCCCTGAAGCGCCTGCCCGACTGGTCCATGC TGTTCGCCGTGATCACCACCATCTTCTCCGTGGCCGAGAAGCAGTGGAC CAACCTGGAGTGGAAGCCCAAGCCCAAGCCCCGCCTGCCCCAGCTGCTG GACGACCACTTCGGCCTGCACGGCCTGGTGTTCCGCCGCACCTTCGCCA TCCGCTCCTACGAGGTGGGCCCCGACCGCTCCACCTCCATCGTGGCCGT GATGAACCACCTGCAGGAGGCCACCCTGAACCACGCCAAGTCCGTGGGC ATCCTGGGCGACGGCTTCGGCACCACCCTGGAGATGTCCAAGCGCGACC TGGCCTGGGTGGTGCGCCGCACCCACGTGGCCGTGGAGCGCTACCCCGC CTGGGGCGACACCGTGGAGGTGGAGGCCTGGGTGGGCGCCTCCGGCAAC ATCGGCATGCGCCGCGACTTCCTGGTGCGCGACTGCAAGACCGGCCACA TCCTGGCCCGCTGCACCTCCGTGTCCGTGATGATGAACGCCCGCACCCG CCGCCTGTCCAAGATCCCCCAGGAGGTGCGCGCCGAGATCGACCCCCTG TTCATCGAGAAGGTGGCCGTGAAGGAGGGCGAGATCAAGAAGCTGCAGA AGTTCAACGACTCCACCGCCGACTACATCCAGGGCGGCTGGACCCCCCG CTGGAACGACCTGGACGTGAACCAGCACGTGAACAACATCAAGTACATC GGCTGGATCTTCAAGTCCGTGCCCGACTCCATCTCCGAGAACCACTACC TGTCCTCCATCACCCTGGAGTACCGCCGCGAGTGCACCCGCGGCTCCGC CCTGCAGTCCCTGACCACCGTGTGCGGCGACTCCTCCGAGGCCGGCATC ATCTGCGAGCACCTGCTGCAGCTGGAGGACGGCCCCGAGGTGCTGCGCG CCCGCACCGAGTGGCGCCCCAAGCTGACCGACTCCTTCCGCGGCATCAT CGTGATCCCCGCCGAGCCCTCCGTGTGA LinderabenzoinFATB1codonoptimizedCDS nucleicacidsequence(LbeFATB1) SEQIDNO:36 ATGGTGACCGCCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCGCCCCCG GCACCTCCCCCAAGCCCGGCAAGTCCTGGCCCTCCTCCCTGTCCCCCTC CTTCAAGCCCAAGTCCATCCCCAACGCCGGCTTCCAGGTGAAGGCCAAC GCCTCCGCCCACCCCAAGGCCAACGGCTCCGCCGTGAACCTGAAGTCCG GCTCCCTGAACACCCAGGAGGACACCTCCTCCTCCCCCCCCCCCCGCGC CTTCCTGAACCAGCTGCCCGACTGGTCCATGCTGCTGACCGCCATCACC ACCGTGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCGCGACCGCAAGT CCAAGCGCCCCGACATGCTGGTGGACTCCGTGGGCTCCAAGTCCATCGT GCTGGACGGCCTGGTGTCCCGCCAGATCTTCTCCATCCGCTCCTACGAG ATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGATGAACCACCTGC AGGAGACCTCCATCAACCACTGCAAGTCCCTGGGCCTGCTGAACGACGG CTTCGGCCGCACCCCCGGCATGTGCAAGAACGACCTGATCTGGGTGCTG ACCAAGATGCAGATCATGGTGAACCGCTACCCCACCTGGGGCGACACCG TGGAGATCAACACCTGGTTCTCCCACTCCGGCAAGATCGGCATGGCCTC CGACTGGCTGATCACCGACTGCAACACCGGCGAGATCCTGATCCGCGCC ACCTCCGTGTGGGCCATGATGAACCAGAAGACCCGCCGCTTCTCCCGCC TGCCCTACGAGGTGCGCCAGGAGCTGACCCCCCACTACGTGGACTCCCC CCACGTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAG ACCGGCGACTCCATCCGCAAGGGCCTGACCCCCAAGTGGAACGACCTGG ACGTGAACCAGCACGTGAACAACGTGAAGTACATCGGCTGGATCCTGGA GTCCATGCCCATCGAGGTGCTGGAGACCCAGGAGCTGTGCTCCCTGACC GTGGAGTACCGCCGCGAGTGCGGCATGGACTCCGTGCTGGAGTCCGTGA CCGCCATGGACCCCTCCGAGGACGGCGGCCTGTCCCAGTACAAGCACCT GCTGCGCCTGGAGGACGGCACCGACATCGTGAAGGGCCGCACCGAGTGG CGCCCCAAGAACGCCGGCACCAACGGCGCCATCTCCACCGCCAAGCCCT CCAACGGCAACTCCGTGTCCTGA CpSADtp_trimmedtransit(plastidtargeting) peptideaminoacidsequence SEQIDNO:37 MATASTFSAFNARCGDLRRSAGSGPRRPARPLPVRAAI Thioesterasetransformingconstruct SEQIDNO:38 gaagagcgcccaatgtttaaacccctcaactgcgacgctgggaaccttc tccgggcaggcgatgtgcgtgggtttgcctccttggcacggctctacac cgtcgagtacgccatgaggcggtgatggctgtgtcggttgccacttcgt ccagagacggcaagtcgtccatcctctgcgtgtgtggcgcgacgctgca gcagtccctctgcagcagatgagcgtgactttggccatttcacgcactc gagtgtacacaatccatttttcttaaagcaaatgactgctgattgacca gatactgtaacgctgatttcgctccagatcgcacagatagcgaccatgt tgctgcgtctgaaaatctggattccgaattcgaccctggcgctccatcc atgcaacagatggcgacacttgttacaattcctgtcacccatcggcatg gagcaggtccacttagattcccgatcacccacgcacatctcgctaatag tcattcgttcgtgtcttcgatcaatctcaagtgagtgtgcatggatctt ggttgacgatgcggtatgggtttgcgccgctggctgcagggtctgccca aggcaagctaacccagctcctctccccgacaatactctcgcaggcaaag ccggtcacttgccttccagattgccaataaactcaattatggcctctgt catgccatccatgggtctgatgaatggtcacgctcgtgtcctgaccgtt ccccagcctctggcgtcccctgccccgcccaccagcccacgccgcgcgg [00001] [00002] tcgccgccaagatcagcgcctccatgacgaacgagacgtccgaccgccc cctggtgcacttcacccccaacaagggctggatgaacgaccccaacggc ctgtggtacgacgagaaggacgccaagtggcacctgtacttccagtaca acccgaacgacaccgtctgggggacgcccttgttctggggccacgccac gtccgacgacctgaccaactgggaggaccagcccatcgccatcgccccg aagcgcaacgactccggcgccttctccggctccatggtggtggactaca acaacacctccggcttcttcaacgacaccatcgacccgcgccagcgctg cgtggccatctggacctacaacaccccggagtccgaggagcagtacatc tcctacagcctggacggcggctacaccttcaccgagtaccagaagaacc ccgtgctggccgccaactccacccagttccgcgacccgaaggtcttctg gtacgagccctcccagaagtggatcatgaccgcggccaagtcccaggac tacaagatcgagatctactcctccgacgacctgaagtcctggaagctgg agtccgcgttcgccaacgagggcttcctcggctaccagtacgagtgccc cggcctgatcgaggtccccaccgagcaggaccccagcaagtcctactgg gtgatgttcatctccatcaaccccggcgccccggccggcggctccttca accagtacttcgtcggcagcttcaacggcacccacttcgaggccttcga caaccagtcccgcgtggtggacttcggcaaggactactacgccctgcag accttcttcaacaccgacccgacctacgggagcgccctgggcatcgcgt gggcctccaactgggagtactccgccttcgtgcccaccaacccctggcg ctcctccatgtccctcgtgcgcaagttctccctcaacaccgagtaccag gccaacccggagacggagctgatcaacctgaaggccgagccgatcctga acatcagcaacgccggcccctggagccggttcgccaccaacaccacgtt gacgaaggccaacagctacaacgtcgacctgtccaacagcaccggcacc ctggagttcgagctggtgtacgccgtcaacaccacccagacgatctcca agtccgtgttcgcggacctctccctctggttcaagggcctggaggaccc cgaggagtacctccgcatgggcttcgaggtgtccgcgtcctccttcttc ctggaccgcgggaacagcaaggtgaagttcgtgaaggagaacccctact tcaccaaccgcatgagcgtgaacaaccagcccttcaagagcgagaacga cctgtcctactacaaggtgtacggcttgctggaccagaacatcctggag ctgtacttcaacgacggcgacgtcgtgtccaccaacacctacttcatga ccaccgggaacgccctgggctccgtgaacatgacgacgggggtggacaa [00003] acgcccgcgcggcgcacctgacctgttctctcgagggcgcctgttctgc cttgcgaaacaagcccctggagcatgcgtgcatgatcgtctctggcgcc ccgccgcgcggtttgtcgccctcgcgggcgccgcggccgcgggggcgca ttgaaattgttgcaaaccccacctgacagattgagggcccaggcaggaa ggcgttgagatggaggtacaggagtcaagtaactgaaagtttttatgat aactaacaacaaagggtcgtttctggccagcgaatgacaagaacaagat tccacatttccgtgtagaggcttgccatcgaatgtgagcgggcgggccg cggacccgacaaaacccttacgacgtggtaagaaaaacgtggcgggcac tgtccctgtagcctgaagaccagcaggagacgatcggaagcatcacagc acaggatccCGCGTCTCGAACAGAGCGCGCAGAGGAACGCTGAAGGTCT CGCCTCTGTCGCACCTCAGCGCGGCATACACCACAATAACCACCTGACG AATGCGCTTGGTTCTTCGTCCATTAGCGAAGCGTCCGGTTCACACACGT GCCACGTTGGCGAGGTGGCAGGTGACAATGATCGGTGGAGCTGATGGTC [00004] gcggcgacctgcgccgctccgccggctccggcccccgccgccccgcccg ccccctgcccgtgcgcgccgccatcaacgcctccgcccaccccaaggcc aacggctccgccgtgaacctgaagtccggctccctgaacacccaggagg acacctcctcctccccccccccccgcgccttcctgaaccagctgcccga ctggtccatgctgctgaccgccatcaccaccgtgttcgtggccgccgag aagcagtggaccatgctggaccgcaagtccaagcgccccgacatgctgg tggactccgtgggcctgaagtccatcgtgcgcgacggcctggtgtcccg ccagtccttctccatccgctcctacgagatcggcgccgaccgcaccgcc tccatcgagaccctgatgaaccacctgcaggagacctccatcaaccact gcaagtccctgggcctgctgaacgacggcttcggccgcacccccggcat gtgcaagaacgacctgatctgggtgctgaccaagatgcagatcatggtg aaccgctaccccacctggggcgacaccgtggagatcaacacctggttct cccagtccggcaagatcggcatgggctccgactggctgatctccgactg caacaccggcgagatcctgatccgcgccacctccgtgtgggccatgatg aaccagaagacccgccgcttctcccgcctgccctacgaggtgcgccagg agctgaccccccacttcgtggactccccccacgtgatcgaggacaacga ccgcaagctgcacaagttcgacgtgaagaccggcgactccatccgcaag ggcctgaccccccgctggaacgacctggacgtgaaccagcacgtgtcca acgtgaagtacatcggctggatcctggagtccatgcccatcgaggtgct ggagacccaggagctgtgctccctgaccgtggagtaccgccgcgagtgc ggcatggactccaagctggagtccgtgaccgccatggacccctccgagg aggacggcgtgcgctcccagtacaaccacctgctgcgcctggaggacgg caccgacgtggtgaagggccgcaccgagtggcgccccaagaacgccggc accaacggcgccatctccaccggcaagacctccaacggcaactccgtgt ccatggactacaaggaccacgacggcgactacaaggaccacgacatcga [00005] gtatcgacacactctggacgctggtcgtgtgatggactgttgccgccac acttgctgccttgacctgtgaatatccctgccgcttttatcaaacagcc tcagtgtgtttgatcttgtgtgtacgcgcttttgcgagttgctagctgc ttgtgctatttgcgaataccacccccagcatccccttccctcgtttcat atcgcttgcatcccaaccgcaacttatctacgctgtcctgctatccctc agcgctgctcctgctcctgctcactgcccctcgcacagccttggtttgg gctccgcctgtattctcctggtactgcaacctgtaaaccagcactgcaa tgctgatgcacgggaagtagtgggatgggaacacaaatggaaagcttga gctccagcgccatgccacgccctttgatggcttcaagtacgattacggt gttggattgtgtgtttgttgcgtagtgtgcatggtttagaataatacac ttgatttcttgctcacggcaatctcggcttgtccgcaggttcaacccca tttcggagtctcaggtcagccgcgcaatgaccagccgctacttcaagga cttgcacgacaacgccgaggtgagctatgtttaggacttgattggaaat tgtcgtcgacgcatattcgcgctccgcgacagcacccaagcaaaatgtc aagtgcgttccgatttgcgtccgcaggtcgatgttgtgatcgtcggcgc cggatccgccggtctgtcctgcgcttacgagctgaccaagcaccctgac gtccgggtacgcgagctgagattcgattagacataaattgaagattaaa cccgtagaaaaatttgatggtcgcgaaactgtgctcgattgcaagaaat tgatcgtcctccactccgcaggtcgccatcatcgagcagggcgttgctc ccggcggcggcgcctggctggggggacagctgttctcggccatgtgtgt acgtagaaggatgaatttcagctggttttcgttgcacagctgtttgtgc atgatttgtttcagactattgttgaatgtttttagatttcttaggatgc atgatttgtctgcatgcgactgaagagc Aminoacidsequenceofregionofdeletionmutant basedonSalFATB2 SEQIDNO:39 LFAVITTIFSVAEKQWTNLEWKPKPKPRLPQL AminoacidsequenceofSalFATB1a(adeletion mutantofSassafrasalbidumSalFATB1) SEQIDNO:40 MATTSLASAFCSMKAVMLARDGRGMKPRSSDLQLRAGNAQTPLKMINGT KESYTESLKRLPDWSMLDDHFGLHGLVERRTFAIRSYEVGPDRSTSIVA VMNHLQEATLNHAKSVGILGDGFGTTLEMSKRDLAWVVRRTHVAVERYP AWGDTVEVECWIGASGNNGMRRDFLVRDCKTGEILTRCTSLSVMMNTRT RRLSKIPEEVRGEIGPLFIDNVAVKDEEIKKLQKLNDSSADYIQGGLTP RWNDLDVNQHVNNIKYVGWILETVPDSIFESHHISSITLEYRRECTRDS VLQSLTTVSGGSLEAGLVCDHLLQLEGGSEVLRARTEWRPKLTDSFRGI IVIPAEPSV AminoacidsequenceofLbeFATB1a(adeletion mutantofLbeFATB1) SEQIDNO:41 MVATSLASAFCSMKAVMLADDGRGMKPRSSDLQLRAGNAQTSLKMIDGT KFSYTESLKRLPDWSKLDDRFGLHGLVFRRTFAIRSYEVGPDRSASILA VLNHLQEATLNHAESVGILGDRFGETLEMSKRDLMWVVRRTYVAVERYP AWGDTVEIESWIGASGNNGMRREFLVRDFKTGEILTRCTSLSVMMNTRT RRLSKIPEEVRGEIGPVFIDNVAVKDEEIKKLQKLNDSTADYIQGGLIP RWNDLDLNQHVNNIKYVSWILETVPDSILESYHMSSITLEYRRECTRDS VLQSLTTVSGGSSEAGLVCEHSLLLEGGSEVLRARTEWRPKLTDSFRGI SVIPAEQSV AminoacidsequenceofmatureSalFATB1a(a deletionmutantofSassafrasalbidumSalFATB1) SEQIDNO:42 GNAQTPLKMINGTKFSYTESLKRLPDWSMLDDHFGLHGLVFRRTFAIRS YEVGPDRSTSIVAVMNHLQEATLNHAKSVGILGDGFGTTLEMSKRDLAW VVRRTHVAVERYPAWGDTVEVECWIGASGNNGMRRDFLVRDCKTGEILT RCTSLSVMMNTRTRRLSKIPEEVRGEIGPLFIDNVAVKDEEIKKLQKLN DSSADYIQGGLTPRWNDLDVNQHVNNIKYVGWILETVPDSIFESHHISS ITLEYRRECTRDSVLQSLTTVSGGSLEAGLVCDHLLQLEGGSEVLRART EWRPKLTDSFRGIIVIPAEPSV AminoacidsequenceofmatureLbeFATB1a(a deletionmutantofLbeFATB1) SEQIDNO:43 GNAQTSLKMIDGTKFSYTESLKRLPDWSKLDDRFGLHGLVFRRTFAIRS YEVGPDRSASILAVLNHLQEATLNHAESVGILGDRFGETLEMSKRDLMW VVRRTYVAVERYPAWGDTVEIESWIGASGNNGMRREFLVRDFKTGEILT RCTSLSVMMNTRTRRLSKIPEEVRGEIGPVFIDNVAVKDEEIKKLQKLN DSTADYIQGGLIPRWNDLDLNQHVNNIKYVSWILETVPDSILESYHMSS ITLEYRRECTRDSVLQSLTTVSGGSSEAGLVCEHSLLLEGGSEVLRART EWRPKLTDSFRGISVIPAEQSV pSZ5176/D4053(SalFATB1a) SEQIDNO:44 [00006] tgcgccgctccgccggctccggcccccgccgccccgcccgccccctgcc cgtgcgcgccgccatcggcaacgcccagacccccctgaagatgatcaac ggcaccaagttctcctacaccgagtccctgaagcgcctgcccgactggt ccatgctggacgaccacttcggcctgcacggcctggtgttccgccgcac cttcgccatccgctcctacgaggtgggccccgaccgctccacctccatc gtggccgtgatgaaccacctgcaggaggccaccctgaaccacgccaagt ccgtgggcatcctgggcgacggcttcggcaccaccctggagatgtccaa gcgcgacctggcctgggtggtgcgccgcacccacgtggccgtggagcgc taccccgcctggggcgacaccgtggaggtggagtgctggatcggcgcct ccggcaacaacggcatgcgccgcgacttcctggtgcgcgactgcaagac cggcgagatcctgacccgctgcacctccctgtccgtgatgatgaacacc cgcacccgccgcctgtccaagatccccgaggaggtgcgcggcgagatcg gccccctgttcatcgacaacgtggccgtgaaggacgaggagatcaagaa gctgcagaagctgaacgactcctccgccgactacatccagggcggcctg accccccgctggaacgacctggacgtgaaccagcacgtgaacaacatca agtacgtgggctggatcctggagaccgtgcccgactccatcttcgagtc ccaccacatctcctccatcaccctggagtaccgccgcgagtgcacccgc gactccgtgctgcagtccctgaccaccgtgtccggcggctccctggagg ccggcctggtgtgcgaccacctgctgcagctggagggcggctccgaggt gctgcgcgcccgcaccgagtggcgccccaagctgaccgactccttccgc ggcatcatcgtgatccccgccgagccctccgtgatggactacaaggacc acgacggcgactacaaggaccacgacatcgactacaaggacgacgacga [00007] pSZ5179/D4056(LbeFATB1a) SEQIDNO:45 [00008] tgcgccgctccgccggctccggcccccgccgccccgcccgccccctgcc cgtgcgcgccgccatcggcaacgcccagacctccctgaagatgatcgac ggcaccaagttctcctacaccgagtccctgaagcgcctgcccgactggt ccaagctggacgaccgcttcggcctgcacggcctggtgttccgccgcac cttcgccatccgctcctacgaggtgggccccgaccgctccgcctccatc ctggccgtgctgaaccacctgcaggaggccaccctgaaccacgccgagt ccgtgggcatcctgggcgaccgcttcggcgagaccctggagatgtccaa gcgcgacctgatgtgggtggtgcgccgcacctacgtggccgtggagcgc taccccgcctggggcgacaccgtggagatcgagtcctggatcggcgcct ccggcaacaacggcatgcgccgcgagttcctggtgcgcgacttcaagac cggcgagatcctgacccgctgcacctccctgtccgtgatgatgaacacc cgcacccgccgcctgtccaagatccccgaggaggtgcgcggcgagatcg gccccgtgttcatcgacaacgtggccgtgaaggacgaggagatcaagaa gctgcagaagctgaacgactccaccgccgactacatccagggcggcctg atcccccgctggaacgacctggacctgaaccagcacgtgaacaacatca agtacgtgtcctggatcctggagaccgtgcccgactccatcctggagtc ctaccacatgtcctccatcaccctggagtaccgccgcgagtgcacccgc gactccgtgctgcagtccctgaccaccgtgtccggcggctcctccgagg ccggcctggtgtgcgagcactccctgctgctggagggcggctccgaggt gctgcgcgcccgcaccgagtggcgccccaagctgaccgactccttccgc ggcatctccgtgatccccgccgagcagtccgtgatggactacaaggacc acgacggcgactacaaggaccacgacatcgactacaaggacgacgacga [00009] CcFATB4Cinnamomumcamphoraacyl-ACPthioesterase CDS. SEQIDNO:46 MVTTSLASAYFSMKAVMLAPDGRGIKPRSSGLQVRAGNERNSCKVINGT KVKDTEGLKGCSTLQGQSMLDDHFGLHGLVFRRTFAIRCYEVGPDRSTS IMAVMNHLQEAARNHAESLGLLGDGFGETLEMSKRDLIWVVRRTHVAVE RYPAWGDTVEVEAWVGASGNTGMRRDFLVRDCKTGHILTRCTSVSVMMN MRTRRLSKIPQEVRAEIDPLFIEKVAVKEGEIKKLQKLNDSTADYIQGG WTPRWNDLDVNQHVNNIIYVGWIFKSVPDSISENHHLSSITLEYRRECT RGNKLQSLTTVCGGSSEAGIICEHLLQLEDGSEVLRARTEWRPKHTDSF QGISERFPQQEPHK DeletedportionofSalFatB1absentfromSalFatB1a SEQIDNO:47 LFAVITTIFSVAEKQWTNLEWKPKPKPRLPQL DeletedportionofLbeFATB1absentfromLbeFATB1a SEQIDNO:48 LLTVITTIFSAAEKQWTNLERKPKPPHL CpauFATB1(transitpeptideappearsinboxedtext) SEQIDNO:49 [00010] AVNLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLTAITTVFVAAEKQW TMRDRKSKRPDMLVDSVGLKSVVLDGLVSRQIFSIRSYEIGADRTASIE TLMNHLQETSINHCKSLGLLNDGFGRTPGMCKNDLIWVLTKMQIMVNRY PTWGDTVEINTWFSHSGKIGMASDWLITDCNTGEILIRATSVWAMMNQK TRRFSRLPYEVRQELTPHYVDSPHVIEDNDRKLHKFDVKTGDSIRKGLT PRWNDLDVNQHVSNVKYIGWILESMPIEVLETQELCSLTVEYRRECGMD SVLESVTAMDPSEDEGRSQYKHLLRLEDGTDIVKGRTEWRPKNAGTNGA ISTAKPSNGNSVS CpauFATB128deletionmutantofCuphea paucipetalaFATB1acyl-ACPthioesterase SEQIDNO:50 [00011] AVNLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLVDSVGLKSVVLDGLV SRQIFSIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTP GMCKNDLIWVLTKMQIMVNRYPTWGDTVEINTWFSHSGKIGMASDWLIT DCNTGEILIRATSVWAMMNQKTRRFSRLPYEVRQELTPHYVDSPHVIED NDRKLHKFDVKTGDSIRKGLTPRWNDLDVNQHVSNVKYIGWILESMPIE VLETQELCSLTVEYRRECGMDSVLESVTAMDPSEDEGRSQYKHLLRLED GTDIVKGRTEWRPKNAGTNGAISTAKPSNGNSVS MatureCpauFATB128deletionmutantofCuphea paucipetalaFATB1acyl-ACPthioesterase SEQIDNO:51 NASAHPKANGSAVNLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLVDSV GLKSVVLDGLVSRQIFSIRSYEIGADRTASIETLMNHLQETSINHCKSL GLLNDGFGRTPGMCKNDLIWVLTKMQIMVNRYPTWGDTVEINTWFSHSG KIGMASDWLITDCNTGEILIRATSVWAMMNQKTRRFSRLPYEVRQELTP HYVDSPHVIEDNDRKLHKFDVKTGDSIRKGLTPRWNDLDVNQHVSNVKY IGWILESMPIEVLETQELCSLTVEYRRECGMDSVLESVTAMDPSEDEGR SQYKHLLRLEDGTDIVKGRTEWRPKNAGTNGAISTAKPSNGNSVS CpauFATB128CDS SEQIDNO:52 [00012] tgcgccgctccgccggctccggcccccgccgccccgcccgccccctgcc cgtgcgcgccgccatcaacgcctccgcccaccccaaggccaacggctcc gccgtgaacctgaagtccggctccctgaacacccaggaggacacctcct cctccccccccccccgcgccttcctgaaccagctgcccgactggtccat gctggtggactccgtgggcctgaagtccgtggtgctggacggcctggtg tcccgccagatcttctccatccgctcctacgagatcggcgccgaccgca ccgcctccatcgagaccctgatgaaccacctgcaggagacctccatcaa ccactgcaagtccctgggcctgctgaacgacggcttcggccgcaccccc ggcatgtgcaagaacgacctgatctgggtgctgaccaagatgcagatca tggtgaaccgctaccccacctggggcgacaccgtggagatcaacacctg gttctcccactccggcaagatcggcatggcctccgactggctgatcacc gactgcaacaccggcgagatcctgatccgcgccacctccgtgtgggcca tgatgaaccagaagacccgccgcttctcccgcctgccctacgaggtgcg ccaggagctgaccccccactacgtggactccccccacgtgatcgaggac aacgaccgcaagctgcacaagttcgacgtgaagaccggcgactccatcc gcaagggcctgaccccccgctggaacgacctggacgtgaaccagcacgt gtccaacgtgaagtacatcggctggatcctggagtccatgcccatcgag gtgctggagacccaggagctgtgctccctgaccgtggagtaccgccgcg agtgcggcatggactccgtgctggagtccgtgaccgccatggacccctc cgaggacgagggccgctcccagtacaagcacctgctgcgcctggaggac ggcaccgacatcgtgaagggccgcaccgagtggcgccccaagaacgccg gcaccaacggcgccatctccaccgccaagccctccaacggcaactccgt gtccatggactacaaggaccacgacggcgactacaaggaccacgacatc [00013] ChFATB2(UniprotQ39514) SEQIDNO:53 MVAAAASSAFFPVPAPGASPKPGKFGNWPSSLSPSFKPKSIPNGGFQVK ANDSAHPKANGSAVSLKSGSLNTQEDTSSSPPPRTFLHQLPDWSRLLTA ITTVFVKSKRPDMHDRKSKRPDMLVDSFGLESTVQDGLVFRQSFSIRSY EIGTDRTASIETLMNHLQETSLNHCKSTGILLDGFGRTLEMCKRDLIWV VIKMQIKVNRYPAWGDTVEINTRFSRLGKIGMGRDWLISDCNTGEILVR ATSAYAMMNQKTRRLSKLPYEVHQEIVPLFVDSPVIEDSDLKVHKFKVK TGDSIQKGLTPGWNDLDVNQHVSNVKYIGWILESMPTEVLETQELCSLA LEYRRECGRDSVLESVTAMDPSKVGVRSQYQHLLRLEDGTAIVNGATEW RPKNAGANGAISTGKTSNGNSVS ChFATB227DeletionmutantofCupheahookeriana FATB2 SEQIDNO:54 [00014] AVSLKSGSLNTQEDTSSSPPPRTFLHQLPDWSRLVDSFGLESTVQDGLV FRQSFSIRSYEIGTDRTASIETLMNHLQETSLNHCKSTGILLDGFGRTL EMCKRDLIWVVIKMQIKVNRYPAWGDTVEINTRFSRLGKIGMGRDWLIS DCNTGEILVRATSAYAMMNQKTRRLSKLPYEVHQEIVPLFVDSPVIEDS DLKVHKFKVKTGDSIQKGLTPGWNDLDVNQHVSNVKYIGWILESMPTEV LETQELCSLALEYRRECGRDSVLESVTAMDPSKVGVRSQYQHLLRLEDG TAIVNGATEWRPKNAGANGAISTGKTSNGNSVS ChFATB227MaturedeletionmutantofCuphea hookerianaFATB2 SEQIDNO:55 NDSAHPKANGSAVSLKSGSLNTQEDTSSSPPPRTFLHQLPDWSRLVDSF GLESTVQDGLVFRQSFSIRSYEIGTDRTASIETLMNHLQETSLNHCKST GILLDGFGRTLEMCKRDLIWVVIKMQIKVNRYPAWGDTVEINTRFSRLG KIGMGRDWLISDCNTGEILVRATSAYAMMNQKTRRLSKLPYEVHQEIVP LFVDSPVIEDSDLKVHKFKVKTGDSIQKGLTPGWNDLDVNQHVSNVKYI GWILESMPTEVLETQELCSLALEYRRECGRDSVLESVTAMDPSKVGVRS QYQHLLRLEDGTAIVNGATEWRPKNAGANGAISTGKTSNGNSVS ChFATB227CDS SEQIDNO:56 [00015] tgcgccgctccgccggctccggcccccgccgccccgcccgccccctgcc cgtgcgcgccgccatcaacgactccgcccaccccaaggccaacggctcc gccgtgagcctgaagtccggcagcctgaacacccaggaggacacctcct ccagcccccccccccgcaccttcctgcaccagctgcccgactggagccg cctggtggacagcttcggcctggagtccaccgtgcaggacggcctggtg ttccgccagtccttctccatccgctcctacgagatcggcaccgaccgca ccgccagcatcgagaccctgatgaaccacctgcaggagacctccctgaa ccactgcaagagcaccggcatcctgctggacggcttcggccgcaccctg gagatgtgcaagcgcgacctgatctgggtggtgatcaagatgcagatca aggtgaaccgctaccccgcctggggcgacaccgtggagatcaacacccg cttcagccgcctgggcaagatcggcatgggccgcgactggctgatctcc gactgcaacaccggcgagatcctggtgcgcgccaccagcgcctacgcca tgatgaaccagaagacccgccgcctgtccaagctgccctacgaggtgca ccaggagatcgtgcccctgttcgtggacagccccgtgatcgaggactcc gacctgaaggtgcacaagttcaaggtgaagaccggcgacagcatccaga agggcctgacccccggctggaacgacctggacgtgaaccagcacgtgtc caacgtgaagtacatcggctggatcctggagagcatgcccaccgaggtg ctggagacccaggagctgtgctccctggccctggagtaccgccgcgagt gcggccgcgactccgtgctggagagcgtgaccgccatggaccccagcaa ggtgggcgtgcgctcccagtaccagcacctgctgcgcctggaggacggc accgccatcgtgaacggcgccaccgagtggcgccccaagaacgccggcg ccaacggcgccatctccaccggcaagaccagcaacggcaactccgtgtc catggactacaaggaccacgacggcgactacaaggaccacgacatcgac [00016] NucleotidesequenceoftransformingDNAcontained inpSZ6315 SEQIDNO:57 caccggcgcgctgcttcgcgtgccgggtgcagcaatcagatccaagtct gacgacttgcgcgcacgcgccggatccttcaattccaaagtgtcgtccg cgtgcgcttcttcgccttcgtcctcttgaacatccagcgacgcaagcgc agggcgctgggcggctggcgtcccgaaccggcctcggcgcacgcggctg aaattgccgatgtcggcaatgtagtgccgctccgcccacctctcaatta agtttttcagcgcgtggttgggaatgatctgcgctcatggggcgaaaga aggggttcagaggtgctttattgttactcgactgggcgtaccagcattc gtgcatgactgattatacatacaaaagtacagctcgcttcaatgccctg cgattcctactcccgagcgagcactcctctcaccgtcgggttgcttccc acgaccacgccggtaagagggtctgtggcctcgcgcccctcgcgagcgc atctttccagccacgtctgtatgattttgcgctcatacgtctggcccgt cgaccccaaaatgacgggatcctgcataatatcgcccgaaatgggatcc aggcattcgtcaggaggcgtcagccccgcgggagatgccggtcccgccg [00017] ggcctgcatctccctgaagggcgtgttcggcgtctccccctcctacaac ggcctgggcctgacgccccagatgggctgggacaactggaacacgttcg cctgcgacgtctccgagcagctgctgctggacacggccgaccgcatctc cgacctgggcctgaaggacatgggctacaagtacatcatcctggacgac tgctggtcctccggccgcgactccgacggcttcctggtcgccgacgagc agaagttccccaacggcatgggccacgtcgccgaccacctgcacaacaa ctccttcctgttcggcatgtactcctccgcgggcgagtacacgtgcgcc ggctaccccggctccctgggccgcgaggaggaggacgcccagttcttcg cgaacaaccgcgtggactacctgaagtacgacaactgctacaacaaggg ccagttcggcacgcccgagatctcctaccaccgctacaaggccatgtcc gacgccctgaacaagacgggccgccccatcttctactccctgtgcaact ggggccaggacctgaccttctactggggctccggcatcgcgaactcctg gcgcatgtccggcgacgtcacggcggagttcacgcgccccgactcccgc tgcccctgcgacggcgacgagtacgactgcaagtacgccggcttccact gctccatcatgaacatcctgaacaaggccgcccccatgggccagaacgc gggcgtcggcggctggaacgacctggacaacctggaggtcggcgtcggc aacctgacggacgacgaggagaaggcgcacttctccatgtgggccatgg tgaagtcccccctgatcatcggcgcgaacgtgaacaacctgaaggcctc ctcctactccatctactcccaggcgtccgtcatcgccatcaaccaggac tccaacggcatccccgccacgcgcgtctggcgctactacgtgtccgaca cggacgagtacggccagggcgagatccagatgtggtccggccccctgga caacggcgaccaggtcgtggcgctgctgaacggcggctccgtgtcccgc cccatgaacacgaccctggaggagatcttcttcgactccaacctgggct ccaagaagctgacctccacctgggacatctacgacctgtgggcgaaccg cgtcgacaactccacggcgtccgccatcctgggccgcaacaagaccgcc accggcatcctgtacaacgccaccgagcagtcctacaaggacggcctgt ccaagaacgacacccgcctgttcggccagaagatcggctccctgtcccc caacgcgatcctgaacacgaccgtccccgcccacggcatcgcgttctac [00018] ctgatgtggcgcggacgccgtcgtactctttcagactttactcttgagg aattgaacctttctcgcttgctggcatgtaaacattggcgcaattaatt gtgtgatgaagaaagggtggcacaagatggatcgcgaatgtacgagatc gacaacgatggtgattgttatgaggggccaaacctggctcaatcttgtc gcatgtccggcgcaatgtgatccagcggcgtgactctcgcaacctggta gtgtgtgcgcaccgggtcgctttgattaaaactgatcgcattgccatcc cgtcaactcacaagcctactctagctcccattgcgcactcgggcgcccg gctcgatcaatgttctgagcggagggcgaagcgtcaggaaatcgtctcg gcagctggaagcgcatggaatgcggagcggagatcgaatcaggatcccg cgtctcgaacagagcgcgcagaggaacgctgaaggtctcgcctctgtcg cacctcagcgcggcatacaccacaataaccacctgacgaatgcgcttgg ttcttcgtccattagcgaagcgtccggttcacacacgtgccacgttggc gaggtggcaggtgacaatgatcggtggagctgatggtcgaaacgttcac [00019] [00020] gcgtgcggggctggcgggagtgggacgccctcctcgctcctctctgttc tgaacggaacaatcggccaccccgcgctacgcgccacgcatcgagcaac gaagaaaaccccccgatgataggttgcggtggctgccgggatatagatc cggccgcacatcaaagggcccctccgccagagaagaagctcctttccca gcagactccttctgctgccaaaacacttctctgtccacagcaacaccaa aggatgaacagatcaacttgcgtctccgcgtagcttcctcggctagcgt gcttgcaacaggtccctgcactattatcttcctgctttcctctgaatta tgcggcaggcgagcgctcgctctggcgagcgctccttcgcgccgccctc gctgatcgagtgtacagtcaatgaatggtgagctccgcgcctgcgcgag gacgcagaacaacgctgccgccgtgtcttttgcacgcgcgactccggcg cttcgctggtggcacccccataaagaaaccctcaattctgtttgtggaa gacacggtgtacccccacccacccacctgcacctctattattggtatta ttgacgcgggagtgggcgttgtaccctacaacgtagcttctctagtttt cagctggctcccaccattgtaaattcatgctagaatagtgcgtggttat gtgagaggtatagtgtgtctgagcagacggggcgggatgcatgtcgtgg tggtgatctttggctcaaggcgtcgtcgacgtgacgtgcccgatcatga gagcaataccgcgctcaaagccgacgcatagcctttactccgcaatcca aacgactgtcgctcgtattttttggatatctattttaaagagcgagcac agcgccgggcatgggcctgaaaggcctcgcggccgtgctcgtggtgggg gccgcgagcgcgtggggcatcgcggcagtgcaccaggcgcagacggagg aacgcatggtgcgtgcgcaatataagatacatgtattgttgtcctgcag g NucleotidesequenceofBnOTE(D124A)inpSZ6316 SEQIDNO:58 [00021] NucleotidesequenceofBnOTE(D209A)inpSZ6317 SEQIDNO:59 [00022] NucleotidesequenceofBnOTE(D124A,D209A)in pSZ6318 SEQIDNO:60 [00023] Aminoacidsequenceofwild-typeBnOTE;positions D124andD209underlined SEQIDNO:61 MATASTFSAFNARCGDLRRSAGSGPRRPARPLPVRGRASQLRKPALDPL RAVISADQGSISPVNSCTPADRLRAGRLMEDGYSYKEKFIVRSYEVGIN KTATVETIANLLQEVACNHVQKCGFSTDGFATTLTMRKLHLIWVTARMH IEIYKYPAWSDVVEIETWCQSEGRIGIRRDWILRDSATNEVIGRATSKW VMMNQDTRRLQRVIDEVRDEYLVFCPREPRLAFPEENNSSLKKIPKLED PAQYSMLELKPRRADLDMNQHVNNVTYIGWVLESIPQEIIDTHELQVIT LDYRRECQQDDIVDSLTTSEIPDDPISKFTGTNGSAMSSIQGHNESQFL HMLRLSENGQEINRGRTQWRKKSSR*