RADIOISOTOPE LABELED COMPOUND FOR IMAGING OR TREATMENT OF PROSTATE CANCER

Abstract

The present invention relates to: a derivative in which glutamate-urea-Lysine (GUL) and isonitrile are linked by a linker; a radioisotope labeled compound comprising the derivative; and a pharmaceutical composition for treating and diagnosing prostate cancer, containing the derivative as an active ingredient. A derivative according to the present invention has high binding capacity for PSMA, which is expressed in prostate cancer, by acting as a multi-ligand through the binding of three or six derivatives to one atom of technetium or rhenium, has excellent stability in human serum when administered in vivo, and is excreted into the kidney rather than the hepatobiliary tract because of high water solubility so that a clear image of a prostate cancer tumor site can be obtained, and thus the present invention can be effectively usable as a pharmaceutical composition for treating or diagnosing prostate cancer.

Claims

1: A compound represented by formula 1 below or a pharmaceutically acceptable salt thereof: ##STR00010## wherein L is a linker.

2: The compound or the pharmaceutically acceptable salt thereof according to claim 1, wherein the linker L includes at least one complex selected from the group consisting of straight or branched C.sub.1-10 alkyl, C.sub.4-10 aryl, urea, thiourea, triazole, monopeptide, dipeptide, tripeptide C.sub.4-10 cycloalkyl, benzyl, halogenated benzyl, phenyl, halogenated phenyl, ether, thioether, amine, amide, ketone, ester, thioester, hydrazine, hydrazide, pentose and hexose.

3: A compound represented by formula 2a or 2b below or a pharmaceutically acceptable salt thereof: ##STR00011## wherein in formula 2a or 2b M is a metal, R.sub.1 is a compound of claim 1, R.sub.2 is R.sub.1 or —CO, and the binding between R.sub.1 and M is that the isonitrile group of R1 binds with M.

4: The compound or the pharmaceutically acceptable salt thereof according to claim 3, wherein the M is copper (Cu), technetium (Tc) or rhenium (Re).

5: The compound or the pharmaceutically acceptable salt thereof according to claim 3, wherein the M is .sup.60Cu, .sup.61Cu, .sup.62Cu, .sup.63Cu, .sup.64Cu, .sup.65Cu, .sup.67Cu, .sup.96Tc, .sup.96mTc, .sup.97mTc, .sup.99mTc, .sup.101Tc, .sup.186Re or .sup.188Re.

6: A pharmaceutical composition for the treatment or diagnosis of prostate cancer containing the compound of claim 1 or a pharmaceutically acceptable salt thereof as an active ingredient.

7: A kit for imaging or treatment of prostate cancer, said kit comprising a pharmaceutically acceptable non-pyrogenic sterile vial containing 0.01 to 100 mg/unit dose of the compound of claim 1, a reducing agent, a buffer, an antioxidant and an adjuvant.

8: The kit for imaging or the treatment of prostate cancer according to claim 7, wherein the reducing agent is at least one selected from the group consisting of stannous chloride, cysteine, sodium borohydride, ascorbic acid or a salt thereof.

9: The kit for imaging or the treatment of prostate cancer according to claim 7, wherein the buffer is at least one selected from the group consisting of acetic acid, gluconate, glucoheptonate, phosphonate, glucarate, tartrate, succinate and citric acid.

10: The kit for imaging or the treatment of prostate cancer according to claim 7, wherein the antioxidant is at least one selected from the group consisting of ascorbic acid or gentisic acid.

11: The kit for imaging or the treatment of prostate cancer according to claim 7, wherein the adjuvant is carbon monoxide.

12: The kit for imaging or the treatment of prostate cancer according to claim 7, wherein the kit father comprises a radioisotope, wherein radioisotope is .sup.60Cu, .sup.61Cu, .sup.62Cu, .sup.63Cu, .sup.64Cu, .sup.65Cu, .sup.67Cu, .sup.96Tc, .sup.96mTc, .sup.97mTc, .sup.99mTc, .sup.101Tc, .sup.186Re or .sup.188Re.

13: A method of treating or diagnosing prostate cancer comprising administering the compound of claim 3 or a pharmaceutically acceptable salt thereof to a subject in need thereof.

14: A pharmaceutical composition for the treatment or diagnosis of prostate cancer containing the compound of claim 3 or a pharmaceutically acceptable salt thereof as an active ingredient.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0037] FIGS. 1A to 1C show the results of HPLC for confirming the labeling efficiency after radioisotope labeling. An Xterra RP18 3.5 μm (4.6 mm×100 mm) column was used, and solvent A (0.1% trifluoroacetic acid (TFA) aqueous solution) and solvent (acetonitrile) were used. A concentration gradient, starting with 100% solvent A at the beginning and reaching 100% solvent B after 30 minutes, was used and the flow rate was 3 mL/min. As a result, [.sup.99mTc][Tc(H.sub.2O).sub.3(CO).sub.3].sup.+ and [.sup.99mTc]Tc-15 and [.sup.99mTc]Tc-16 according to the present invention are shown in FIGS. 1A, 1B, and 1C, respectively, and the labeling efficiency of [.sup.99mTc]Tc-15 and [.sup.99mTc]Tc-16 can be seen to be almost 100%.

[0038] FIGS. 2A and 2B show the results of a stability test in which [.sup.99mTc]Tc-15 and [.sup.99mTc]Tc-16 according to the present invention were incubated with human serum at 37° C. for 6 hours. The results for [.sup.99mTc]Tc-15 and [.sup.99mTc]Tc-16 are shown in FIGS. 2A and 2B, respectively, and both showed stability of 98% or more even after 6 hours.

[0039] FIGS. 3A and 3B show the saturation binding curves according to the in vitro cell binding test results of [.sup.99mTc]Tc-15 and [.sup.99mTc]Tc-16 according to the present invention, respectively. As a result of calculating the Kd values of [.sup.99mTc]Tc-15 and [.sup.99mTc]Tc-16 by nonlinear regression analysis, 5.5 nM and 0.2 nM were obtained, respectively.

[0040] FIGS. 4A and 4B are SPECT images 1 hour and 3 hours after the intravenous injection of [.sup.99mTc]Tc-15 according to the present invention into 22Rv1 cell-transplanted mice. As shown in FIG. 4A, the cancer tumor was located on the right shoulder indicated by the arrow, and although the radioactivity was slightly increased due to high radioactivity in the lower kidney, no clear intake was observed at both 1 hour and 3 hours after the injection. As shown in FIG. 4B, [.sup.99mTc]Tc-15 was not observed in the cancer tumor by injecting MIP-1072 with [.sup.99mTc]Tc-15 to selectively block PSMA.

[0041] FIGS. 5A and 5B are SPECT images 1 hour and 4 hours after the intravenous injection of [.sup.99mTc]Tc-16 according to the present invention into 22Rv1 cell-transplanted mice. As shown in FIG. 5, the cancer tumor was located on the right shoulder indicated by the arrow, and although the radioactivity was high in the lower kidney, the increase in radioactivity was evident even 1 hour after the injection. In particular, 4 hours after the injection, the surrounding radioactivity was low, and the intake was more distinct. As shown in FIG. 5B, [.sup.99mTc]Tc-16 was not observed in the cancer tumor by injecting MIP-1072 with [.sup.99mTc]Tc-16 to selectively block PSMA.

DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0042] Hereinafter, the present invention is described in detail.

[0043] The embodiments of this invention can be modified in various other forms, and the scope of the present invention is not limited to the embodiments described below. It is well understood by those in the art who has the average knowledge on this field that the embodiments of the present invention are given to explain the present invention more precisely. In addition, the “inclusion” of an element throughout the specification does not exclude other elements, but may include other elements, unless specifically stated otherwise.

[0044] The present invention provides a compound represented by the following formula 1 or a pharmaceutically acceptable salt or a complex thereof.

##STR00004##

[0045] (In formula 1 above, L is a linker).

[0046] The linker L can include at least one complex selected from the group consisting of straight or branched C.sub.1-10 alkyl, C.sub.4-10 aryl, urea, thiourea, triazole, monopeptide, dipeptide, tripeptide C.sub.4-10 cycloalkyl, benzyl, halogenated benzyl, phenyl, halogenated phenyl, ether, thioether, amine, amide, ketone, ester, thioester, hydrazine, hydrazide, pentose and hexose.

[0047] The linker connects the GUL portion that binds to prostate cancer cells and the isonitrile group that forms a complex with technetium. The GUL portion composed of Glutamate-urea-Lysine has a property of strongly binding to PSMA, an antigen that is frequently expressed in prostate cancer cells. Therefore, using such property, various bifunctional chelating agents can be combined with a linker to label a metallic radioisotope and used as a radiopharmaceutical, and such patents include KR 10-1639599, US 2012/0269726 A1, etc. These patents are all composed of chelating agents that are hard bases, so they are good for labeling metallic isotopes belonging to hard acids such as .sup.68Ga, .sup.111in, .sup.177Lu and .sup.90Y. In the present invention, an isonitrile group belonging to a soft base was introduced to be good for labeling such metallic isotopes as .sup.99mTc, .sup.188Re and .sup.186Re, which are the most widely used and marketable soft acids in nuclear medicine. In principle, any chemical bond may be used for the linker connecting GUL and the isonitrile group. However, if there is a group such as benzyl or benzyl iodide that can bind to albumin, the blood concentration can be maintained high for a long time, so it is more preferable because it can reduce kidney intake and increase cancer cell intake.

[0048] The present invention also provides a radioisotope-labeled compound represented by the following formula 2 or a pharmaceutically acceptable salt thereof.

##STR00005##

[0049] (In formula 2 above,

[0050] M is a radioactive metal,

[0051] R.sub.1 is

##STR00006##

and

[0052] R.sub.2 is R.sub.1 or —CO).

[0053] At this time, the binding between R.sub.1 and M is that the isonitrile group of R1 binds with M.

[0054] It is known that technetium or rhenium having an oxidation number of +1 binds to a ligand having 6 isonitrile groups or to a ligand having 3 CO molecules and 3 isonitrile groups to form a stable complex. Therefore, by forming an octahedral structure as shown in formula 2 above, a multivalent complex including 3 or 6 GULs can be synthesized. This multivalent structure is known to increase the targeting efficiency when administered in the body, especially due to increased binding force.

[0055] The present invention also provides a pharmaceutical composition for the treatment or diagnosis of prostate cancer containing the labeled compound represented by formula 2 as an active ingredient. For the diagnosis, .sup.99mTc, the most widely used radioactive isotope in nuclear medicine, is used, and for the treatment, .sup.188Re or .sup.186Re is used.

[0056] M may be copper (Cu), technetium (Tc) or rhenium (Re).

[0057] In another aspect, M may be .sup.60Cu, .sup.61Cu, .sup.62Cu, .sup.63Cu, .sup.64Cu, .sup.65Cu, .sup.67Cu, .sup.96Tc, .sup.96mTc, .sup.97mTc, .sup.99mTc, .sup.101Tc, .sup.186Re or .sup.188Re.

[0058] The present invention also provides a radiopharmaceutical for imaging of prostate cancer containing the compound represented by formula 1 or formula 2 or the pharmaceutically acceptable salt thereof as an active ingredient. The radiopharmaceutical for imaging of prostate cancer includes a composition for the treatment or diagnosis of prostate cancer.

[0059] The present invention also provides a kit for the treatment or diagnosis of prostate cancer containing the labeled compound represented by Formula 2 as an active ingredient including the compound represented by formula 1 or the pharmaceutically acceptable salt thereof.

[0060] The compound represented by formula 1 of the present invention can be used as a form of a pharmaceutically acceptable salt, in which the salt is preferably acid addition salt formed by pharmaceutically acceptable free acids. The pharmaceutically acceptable salt refers to any organic or inorganic addition salt of the basic compound of formula 1 at a concentration having an effective action that is relatively non-toxic and harmless to the patient, and the side effects due to this salt do not reduce the beneficial efficacy of the basic compound of formula 1. For these salts, inorganic acids and organic acids can be used as free acids. Hydrochloric acid, bromic acid, nitric acid, sulfuric acid, perchloric acid, phosphoric acid, and the like can be used as inorganic acids, and citric acid, acetic acid, lactic acid, maleic acid, fumaric acid, gluconic acid, methanesulfonic acid, glycolic acid, succinic acid, tartaric acid, galacturonic acid, embonic acid, glutamic acid, aspartic acid, oxalic acid (D) or (L) malic acid, maleic acid, methanesulfonic acid, ethanesulfonic acid, 4-toluenesulfonic acid, salicylic acid, citric acid, benzoic acid, malonic acid, and the like can be used as organic acids. In addition, these salts include alkali metal salts (sodium salt, potassium salt, etc.) and alkaline earth metal salts (calcium salt, magnesium salt, etc.) and the like. For example, acetate, aspartate, benzate, besylate, bicarbonate/carbonate, bisulfate/sulfate, borate, camsylate, citrate, edisylate, esylate, formate, fumarate, gluceptate, gluconate, glucuronate, hexafluorophosphate, hybenzate, hydrochloride/chloride, hydrobromide/bromide, hydroiodide/iodide, isethionate, lactate, malate, maleate, malonate, mesylate, methylsulfate, naphthylate, 2-naphthylate, nicotinate, nitrate, orotate, oxalate, palmitate, pamoate, phosphate/hydrogen phosphate/dihydrogen phosphate, saccharate, stearate, succinate, tartrate, tosylate, trifluoroacetate, aluminum, arginine, benzathine, calcium, choline, diethylamine, diolamine, glycine, lysine, magnesium, meglumine, olamine, potassium, sodium, copper, tromethamine, zinc salt, and the like can be included as the acid addition salt, of which hydrochloride or +1-valent copper is preferable.

[0061] In addition, the compound represented by formula 1 of the present invention includes all salts, isomers, hydrates, solvates and complexes that can be prepared by conventional methods as well as the pharmaceutically acceptable salts.

[0062] The addition salt according to the present invention can be prepared by the conventional method known to those in the art. For example, the compound of formula 1 is dissolved in water-miscible organic solvent such as acetone, methanol, ethanol, or acetonitrile, to which excessive organic acid or acid aqueous solution of inorganic acid is added to induce precipitation or crystallization. Then, the solvent or the excessive acid is evaporated from the mixture, followed by drying the mixture to give addition salt or suction-filtering the precipitated salt to give the same.

[0063] The present invention also provides a labeled compound represented by formula 2 in which a metallic radioisotope is coordinated to a compound represented by formula 1 or a pharmaceutically acceptable salt thereof.

[0064] The metallic radioisotope is preferably .sup.99mTc, .sup.188Re, or .sup.186Re.

[0065] The present invention also provides a pharmaceutical composition for the treatment or diagnosis of prostate cancer containing the labeled compound as an active ingredient. In addition, the present invention provides a radiopharmaceutical for imaging of prostate cancer containing the labeled compound as an active ingredient.

[0066] Radioisotopes mainly include alpha-ray-emitting nuclides, beta-ray-emitting nuclides, gamma-ray-emitting nuclides, and positron-emitting nuclides. Among them, alpha-ray and beta-ray-emitting nuclides are used for treatment, and gamma-ray and positron-emitting nuclides are used for diagnosis by nuclear imaging. When the pharmaceutical composition according to the present invention is used for imaging of prostate cancer, the radioactive isotope of the labeled compound is preferably .sup.99mTc, and when used for the treatment of prostate cancer, it is preferably .sup.188Re or .sup.186Re.

[0067] When administered to the body, the pharmaceutical composition according to the present invention has excellent stability in human serum, binds well to PSMA competitively expressed in prostate cancer, and has excellent PSMA inhibitory efficacy at low concentrations. In addition, since the composition is highly water-soluble, it is excreted by the kidney rather than the hepatobiliary tract, and is ingested into the prostate cancer tissue to emit radiation to the prostate cancer tumor site. Therefore, the composition of the present invention can be effectively used as a pharmaceutical composition for the treatment or diagnosis of prostate cancer.

[0068] The present invention provides a kit for the treatment or diagnosis of prostate cancer labeled with a metallic radioisotope in a pharmaceutically acceptable non-pyrogenic sterile form, containing the compound represented by formula 1 or the pharmaceutically acceptable salt thereof.

[0069] Specifically, the kit for the treatment or diagnosis of prostate cancer according to the present invention contains 10 ng to 100 mg of the compound represented by formula 1 above.

[0070] In order to conveniently label the metallic radioisotope to the compound represented by formula 1, the kit is prepared by adding the compound represented by formula 1, an appropriate buffer solution, and a reducing agent in a liquid state in advance, dispensing it into pharmaceutically desirable sterile vials and sealing thereof. It can be stored in refrigeration, freezing or freeze-drying, and can be usefully used when necessary.

[0071] At this time, the kit can be prepared by adding 0.01 mL to 10 mL of a buffer solution having a pH of 1 to 9 and a concentration of 1 μM to 10 M to control the hydrogen ion concentration during isotope labeling, and sealing thereof in a dissolved state, frozen state or freeze-dried state.

[0072] In addition, as the buffer solution, acetic acid, phosphoric acid, citric acid, fumaric acid, ascorbic acid, butyric acid, succinic acid, tartaric acid, carbonic acid, glucoheptonic acid, gluconic acid, glucuronic acid, glucaric acid, boric acid or a sodium salt or potassium salt thereof is preferred.

[0073] The kit of the present invention can include a reducing agent for reducing technetium or rhenium in a +7-valent state to a +1-valent state. As the reducing agent, tin chloride is most widely used, and NaBH4, ascorbic acid and the like can also be used.

[0074] Further, the kit of the present invention can additionally include an antioxidant. The antioxidant is to prevent the compound represented by formula 1 labeled with a radioactive isotope from being deteriorated by radiation decomposition. The antioxidant is preferably vitamin C or gentisic acid, and the kit of the present invention preferably contains 0 to 500 mg per unit dose.

[0075] The kit can be supplemented with buffered sterile vials, saline, syringes, filters, columns and other auxiliary devices to prepare injectable solutions for use by a clinical pathologist or technician. It is well known to those in the art that the kit can be changed and modified according to the patient's individual needs or diet, as well as changes in the form in which the radioisotope can be provided or obtained.

[0076] In addition, a radioisotope of 0.1 to 500.1 to 500 mCi per 1 mg of the compound represented by formula 1 can be added to the kit immediately before use and reacting for 0.1 to 30 minutes to prepare a radioisotope-labeled compound.

[0077] Herein after, the present invention will be described in detail by the following examples and experimental examples.

[0078] However, the following examples and experimental examples are only for illustrating the present invention, and the contents of the present invention are not limited thereto.

[0079] Non-Radioactive Compounds Purified by RP-HPLC

[0080] RP-HPLC was performed using a Gilson, 506C system interface, a 155 UV/VIS detector (dual wavelength 220, 254 nm) and 321 pumps. Operation of the Gilson HPLC system was controlled using Trilution software. Purification of intermediates and final compounds was performed using a semi-preparative XTerra RP18 10 μm (10 mm×250 mm) column (Waters Co., U.S.A). The mobile phase consisted of 0.1% TFA/water (solvent A) and acetonitrile (solvent B) (gradient method), and the flow rate was as follow: 5 mL/min 0-40 min, 0-100% B (method 1), 3 mL/min, 0-5 min, 0% B; 5-30 min, 0-100% B (method 2), 5 mL/min, 0-35 min, 0-100% B (method 3), 5 mL/min, 0-40 min, 0-100% B (method 4), 3 mL/min, water (solvent A), acetonitrile (solvent B) 0-5 min gradient method, 3 mL/min, 0% B; 5-40 min, 0-100% B (method 5), or 0-30 min, 10-100% B (method 6).

<Example 1> Preparation of (3S)-22-isocyano-5,13,20-trioxo-4,6,12,19-tetrazazadocosane-1,3,7-tricarboxylic Acid (15)

[0081] The GUL-NC compound 15 was prepared according to reaction formulas 1 and 2 below.

##STR00007##

[0082] .sup.aReagents and reaction conditions: (a) Cbz-Lys-Ot-Bu, triphosgene, −78° C. RT, 18 h (b) 10% Pd/C, methanol, 18 h (c) Fmoc-6-Ahx-OH, HBTU, DIPEA, 0° C. RT, overnight (d) 20% piperidine/DMF, 1 h (e) TFA:DCM (1:1), 18 h.

##STR00008##

[0083] .sup.aReagents and reaction conditions (a) formic acid, acetic anhydride, refluxed, 3 h (b) 2,3,5,6-tetrafluorophenol, DCC, DMF, RT, 24 h (c) triphosgene, DCM, TEA, 0° C., 1.5 h (d) 5, DIPEA, rt, 5 h (e) 10, DIPEA, rt, 5 h (f) [Re(CO).sub.3(H.sub.2O).sub.3]Br (g) [.sup.99mTc]Tc(H.sub.2O).sub.3(CO).sub.3].sup.+.

Step 1-1: Preparation of tri-tert-butyl (13S)-3,11-dioxo-1-phenyl-2-oxa-4,10,12-triazapentadecane-9,13,15-tricarboxylate (1)

[0084] L-glutamic acid di-tert-butyl ester hydrochloride (2.3 g, 7.88 mmol) dissolved in anhydrous DOM (60 mL) was added to TEA (2.6 mL, 25.7 mmol) at 78° C., followed by stirring in a nitrogen environment for 30 minutes. Triphosgene (0.8 g, 2.6 mmol) was added thereto dropwise for one hour, and after stirring the mixture at room temperature for one hour, N′-Cbz-L-lysine-tert-butyl ester (1.6 g, 4.7 mmol) in DOM (20 mL) containing TEA (0.4 mL, 4.7 mmol) was added. The mixture was reacted overnight (18 hours). Upon completion of the reaction, the organic layer was washed with saturated NaHCO.sub.3 (1×50 mL) and water (2×50 mL), and finally washed with brine (1×30 mL). The organic layer was separated, dried over anhydrous sodium sulfate, concentrated under reduced pressure, and purified by silica gel column chromatography using DCM and methanol (95:5, v/v) to give a target compound as a colorless oil (1.9 g, 66%).

[0085] .sup.1H NMR (500 MHz, CDCl.sub.3) δ 7.37-7.27 (m, 5H), 5.13-5.07 (m, 2H), 4.32 (td, J=8.4, 4.8 Hz, 2H), 3.23-3.10 (m, 2H), 2.33-2.23 (m, 2H), 1.90-1.70 (m, 2H), 1.66-1.46 (m, 4H), 1.46-1.26 (m, 31H). ESI-MS, (m/z): [M+H].sup.+, 622.

Step 1-2: Preparation of di-tert-butyl ((6-amino-1-(tert-butoxy)-1-oxohexane-2-yl)carbamoyl)-L-glutamate (2)

[0086] 10% Pd/C (100 mg) was added to a solution of the compound 1 (1.5 g, 2.4 mmol) in methanol (20 mL). The suspension was stirred in a hydrogen environment for 18 hours. Upon completion of the reaction, Pd/C was removed by passing the reactant through Celite®. The obtained filtrate was concentrated under reduced pressure to give a target compound as a colorless oil that slowly hardened for a certain period of time (quantitative yield). Then, the next step was carried out without further purification.

[0087] .sup.1H NMR (500 MHz, CDCl.sub.3) δ 4.31-4.22 (m, 2H), 2.34-2.30 (m, 2H), 1.89-1.77 (m, 2H), 1.77-1.62 (m, 4H), 1.58-1.47 (m, 2H), 1.46-1.28 (m, 29H). ESI-MS, (m/z): [M+H].sup.+, 488.

Step 1-3: Preparation of tri-tert-butyl (20S)-1-(9H-fluorene-9-yl)-3,10,18-trioxo-2-oxa-4,11,17,19-tetrazazadocosane-16,20,22-tricarboxylate (3)

[0088] Fmoc-6-Ahx-OH (505 mg, 1.43 mmol) in DMF (15 mL) and DIPEA (1 mL, 3.5 mmol) were added to a solution of the compound 2 (500 mg, 1.0 mmol) and stirred at 0° C. for 10 minutes in an inert environment. HBTU (585 mg, 1.5 mmol) in DMF (5 mL) was added dropwise thereto, and the reaction mixture was stirred at room temperature for 18 hours. Upon completion of the reaction, ethyl acetate (50 mL) was added thereto, and the organic layer was washed with water (3×30 mL), dried over Na.sub.2SO.sub.4, and then concentrated under reduced pressure. The product was purified by silica gel column chromatography using DCM/methanol (97:3, v/v) to give the product 3 as a white solid (494 mg, 60%).

[0089] .sup.1H NMR (500 MHz, CDCl.sub.3) δ 7.78-7.74 (m, 2H), 7.60 (d, J=7.4 Hz, 2H), 7.42-7.37 (m, 2H), 7.31 (td, J=7.5, 1.2 Hz, 2H), 4.39 (dt, J=10.1, 5.2 Hz, 2H), 4.36-4.26 (m, 2H), 4.25-4.18 (m, 1H), 3.38-3.06 (m, 4H), 2.32 (tdd, J=13.4, 9.3, 6.3 Hz, 2H), 2.19 (dd, J=13.8, 6.6 Hz, 2H), 2.11-2.03 (m, 2H), 1.88-1.75 (m, 2H), 1.58-1.33 (m, 37H). ESI-MS, (m/z): [M+H].sup.+, 823.

Step 1-4: Preparation of di-tert-butyl ((6-(6-aminohexaneamido)-1-(tert-butoxy)-1-oxohexane-2-yl)carbamoyl)-L-glutamate (4)

[0090] A solution of the compound 3 (500 mg, 0.6 mmol) was dissolved in 20% piperidine/DMF (1 mL), followed by stirring at room temperature for one hour. The solvent was removed in vacuo, and the product was purified by HPLC (method 1) to give the product 4 as a white solid (306 mg, 80%).

[0091] .sup.1H NMR (500 MHz, CDCl.sub.3) δ 4.18 (p, J=5.0 Hz, 2H), 2.32 (t, J=7.5 Hz, 2H), 2.28-2.22 (m, 2H), 2.09-1.85 (m, 2H), 1.69 (ddd, J=24.0, 18.3, 11.1 Hz, 6H), 1.58-1.23 (m, 37H). ESI-MS, (m/z): [M+H].sup.+, 601.

Step 1-5: Preparation of ((5-(6-aminohexaneamido)-1-carboxypentyl)carbamoyl)-L-glutamate (5)

[0092] The product 4 (200 mg, 0.33 mmol) was dissolved in a mixture of TFA and DCM (2 mL, 1:1 v/v) and then stirred at room temperature overnight. Upon completion of the reaction, the solvent was removed under reduced pressure, and the residue was purified by HPLC (method 2) to give the product 5 as a white solid (56, 39%).

[0093] .sup.1H NMR (500 MHz, Methanol-d.sub.4) δ 4.27 (ddd, J 31.1, 8.6, 5.0 Hz, 2H), 3.17 (t, J=6.8 Hz, 2H), 2.91 (t, J=7.6 Hz, 2H), 2.40 (ddd, J=8.5, 6.8, 4.2 Hz, 2H), 2.20 (t, J=7.3 Hz, 2H), 1.71-1.60 (m, 10H), 1.45-1.35 (m, 4H). (ESI-MS, (m/z): [M+H].sup.+, 433.

Step 2-1: Preparation of 3-formamidopropanoic Acid (12)

[0094] Acetic anhydride (25 mL) was added dropwise to a solution of b-alanine (5 g, 48.5 mmol) in formic acid (40 mL). After the mixture was heated for 3 hours, the solvent was removed in vacuo and cooled diethyl ether (30 mL) was added to give a solidified oily residue. After the mixture was heated for 3 hours, the solvent was removed in vacuo and cooled diethyl ether (30 mL) was added to give a solidified oily residue. The residue was purified by silica gel chromatography (DCM:methanol, 4:1, v/v) to give the compound 12 as a white solid (3 g, 55%).

[0095] .sup.1H NMR (300 MHz, DMSO-d.sub.6) δ 7.98 (s, 1H), 3.26 (t, J=6.8, 2H), 2.39 (t, J=6.8 Hz, 2H).

Step 2-2: Preparation of 2,3,5,6-tetrafluorophenyl 3-formamidopropanoate (13)

[0096] A solution of the compound 12 (500 mg, 4.27 mmol) was mixed with TFP (780 mg, 4.7 mmol) dissolved in anhydrous DMF (10 mL), and then cooled to 0° C. DCC in DMF (3 mL) was added dropwise for 10 minutes. The reaction mixture was stirred in an ice bath for 10 minutes and then stirred at room temperature for 24 hours. The resulting N,N-dicyclehexylurea was removed by filtration, and ethyl acetate (50 mL) was added to the filtrate. The organic layer was washed with water (40 mL×3), dried over Na.sub.2SO.sub.4 and then concentrated in vacuo. The product was purified by silica gel chromatography (ethyl acetate:hexane, 3:2, v/v) to give the product 13 as a white solid (680 mg, 60%).

[0097] .sup.1H NMR (300 MHz, CDCl.sub.3) δ 8.21 (s, 1H), 7.09-6.95 (m, 1H), 3.72 (t, 5.9, 2H), 2.99 (t, J=5.9 Hz, 2H).

Step 2-3: Preparation of 2,3,5,6-tetrafluorophenyl 3-isocyanopropanoate (14)

[0098] The compound 13 (2 g, 7.7 mmol) was dissolved in ice-cold anhydrous DCM (50 mL), and TEA (2.7 mL, 19.25 mmol) was added thereto, followed by stirring for 10 minutes in a nitrogen environment. Triphosgene (913 mg, 3.1 mmol) was added dropwise thereto over 30 minutes. The mixture was stirred at 0° C. for 1.5 h. Upon completion of the reaction, DCM (20 mL) was added and the organic layer was washed sequentially with saturated NaHCO.sub.3 (50 mL×1), water (50 mL×2) and brine (20 mL×1). The separated organic layer was dried over Na.sub.2SO.sub.4 and concentrated under reduced pressure. The residue was purified by silica gel column chromatography using hexane and ethyl acetate (4:1, v/v) to give the product 14 as a Light yellow solid (1.2 g, 60%).

[0099] .sup.1H NMR (300 MHz, Chloroform-d) δ 6.98 (m, 1H), 3.77 (t, J=6.8 Hz, 2H), 3.07 (t, J=6.8 Hz, 2H).

Step 2-4: Preparation of (3S)-22-isocyano-5,13,20-trioxo-4,6,12,19-tetrazazadocosane-1,3,7-tricarboxylic Acid (15)

[0100] A solution of the compound 5 (90 mg, 0.2 mmol) and DIPEA (2.5 eq) in anhydrous methanol (2 mL) was stirred at room temperature for 10 minutes. The compound 14 (77 mg, 0.3 mmol) was dissolved in anhydrous methanol (1 mL) and then added dropwise thereto, followed by stirring in a nitrogen environment for 5 hours. After the solvent was removed using a vacuum concentrator, the product was purified by RP-HPLC (method 5), and lyophilized under reduced pressure to give the product 15 as a white solid (35 mg, 34%).

[0101] .sup.1H NMR (500 MHz, Methanol-d4) δ 4.23 (ddd, J=18.8, 8.6, 4.9 Hz, 2H), 3.13 (t, J=6.7 Hz, 2H), 2.87 (t, J=7.7 Hz, 2H), 2.40-2.31 (m, 2H), 2.16 (t, J=7.3 Hz, 2H), 1.90-1.72 (m, 2H), 1.66-1.27 (m, 14H). ESI-MS, (m/z): [M+H]+, 514.

<Example 2> Preparation of (3S,21R)-21,24-dibenzyl-35-isocyano-5,13,20,23,26,33-hexaoxo-4,6,12,19,22,25,32-heptaazapentatriacontane-1,3,7-tricarboxylic Acid (16)

[0102] The GUL-NC compound 16 was prepared according to reaction formulas 2 and 3 below.

##STR00009##

[0103] Reagents and reaction conditions (a) Fmoc-Phe-OH, HBTU, DIPEA, 0° C.-RT overnight (b) 20% piperidine/DMF, 1 h (c) Fmoc-Phe-OH, HBTU, DIPEA, 0° C.-RT overnight (d) 20% piperidine/DMF, 1 h (e) Boc-6-Ahx-OH, HBTU, DIPEA, 0° C.-RT overnight (f) TFA:DCM (1:1, v/v).

Step 3-1: Preparation of tri-tert-butyl (23S)-5-benzyl-1-(9H-fluorene-9-yl)-3,6,13,21-tetraoxo-2-oxa-4,7,14,20,22-pentaazapentacosane-19,23,25-tricarboxylate (6)

[0104] The compound 4 (800 mg, 1.33 mmol) was dissolved in a mixture of Fmoc-Phe-OH (515 mg, 1.33) and (0.8 mL, 4.65 mmol) in DMF (6 mL), cooled to 0° C., and stirred for 10 minutes. HBTU (1.0 g, 2.7 mmol) in DMF (2 mL) was added dropwise thereto, and then the reaction mixture was stirred at room temperature for 18 hours. Upon completion of the reaction, ethyl acetate (20 mL) was added thereto, and the organic layer was washed with water (3×40 mL), dried over Na.sub.2SO.sub.4 and concentrated under reduced pressure. The product was purified by silica gel column chromatography using DOM and methanol (96:4, v/v) to give a target compound as a white solid (839 mg, 65%).

[0105] .sup.1H NMR (500 MHz, CDCl.sub.3) δ 7.75 (dt, J=7.6, 0.9 Hz, 2H), 7.39 (t, J=7.4 Hz, 2H), 7.30-7.20 (m, 9H), 4.52-4.34 (m, 4H), 4.30 (t, J=9.1 Hz, 1H), 4.18 (t, J=7.1 Hz, 1H), 3.17 (d, J=6.4 Hz, 2H), 3.06 (d, J=7.2 Hz, 2H), 2.37-2.27 (m, 2H), 2.26-2.01 (m, 4H), 1.89-1.74 (m, 2H), 1.68-1.56 (m, 2H), 1.55-1.22 (m, 37H). ESI-MS, (m/z): [M+H].sup.+, 971.

Step 3-2: Preparation of tri-tert-butyl (35)-21-amino-5,13,20-trioxo-22-phenyl-4,6,12,19-tetrazazadocosane-1,3,7-tricarboxylate (7)

[0106] The compound 6 (300 mg, 0.3 mmol) was dissolved in 20% piperidine/DMF (1 mL) and stirred at room temperature for 1 hour. The solvent was removed in vacuo and purified by HPLC (method 3) to give a target compound as a white solid (179 mg, 80%).

[0107] .sup.1H NMR (500 MHz, CDCl.sub.3) δ 7.28 (d, J=1.8 Hz, 1H), 7.26-7.20 (m, 4H), 4.33 (t, J=7.3 Hz, 1H), 4.24 (dt, J=8.7, 5.3 Hz, 2H), 3.35-3.08 (m, 6H), 2.31 (td, J=7.4, 6.6, 1.7 Hz, 2H), 2.16 (t, J=6.9 Hz, 2H), 1.66-1.50 (m, 4H), 1.49-1.28 (m, 37H). ESI-MS, (m/z): [M+H].sup.+, 748.

Step 3-3: Preparation of tri-tert-butyl (8R,26S)-5,8-dibenzyl-1-(9H-fluorene-9-yl)-3,6,9,16,24-pentaoxo-2-oxa-4,7,10,17,23,25-hexaazaoctacosane-22,26,28-tricarboxylate (8)

[0108] The compound 7 (526 mg, 0.7 mmol) was dissolved in a mixture of Fmoc-Phe-OH (299 mg, 0.7 mmol) and DIPEA (0.43 mL, 4.65 mmol) in DMF (3 mL), cooled to 0° C., and stirred for 10 minutes. HBTU (530 mg, 1.4 mmol) in DMF (2 mL) was added dropwise thereto, and then the reaction mixture was stirred at room temperature for 18 hours. Upon completion of the reaction, ethyl acetate (30 mL) was added thereto, and the organic layer was washed with water (3×40 mL), dried over Na.sub.2SO.sub.4 and concentrated under reduced pressure. The product was purified by silica gel column chromatography using DCM and methanol (96:3, v/v) to give the compound 8 as a white solid (531 mg, 68%).

[0109] .sup.1H NMR (500 MHz, CDCl.sub.3) δ 7.51-7.46 (m, 2H), 7.39 (t, J=7.4 Hz, 2H), 7.30-7.11 (m, 14H), 4.68 (d, J=7.7 Hz, 1H), 4.51-4.29 (m, 4H), 4.21 (t, J=8.9 Hz, 1H), 4.14 (t, J=7.2 Hz, 1H), 3.29-2.80 (m, 8H), 2.37-2.21 (m, 4H), 2.10-2.01 (m, 2H), 1.89-1.74 (m, 2H), 1.62-1.26 (m, 37H). ESI-MS, (m/z): [M+H].sup.+, 1118.

Step 3-4: Preparation of tri-tert-butyl (3S,21R)-24-amino-21-benzyl-5,13,20,23-tetraoxo-25-phenyl-4,6,12,19,22-pentaazapentacosane-1,3,7-tricarboxylate (9)

[0110] The compound 8 (300 mg, 0.3 mmol) was dissolved in 20% piperidine/DMF solution (1 mL) by stirring at room temperature for 1 hour. The solvent was removed in vacuo and the product was purified by HPLC (method 4) to give the product 9 as a white solid (215 mg, 90%).

[0111] .sup.1H NMR (500 MHz, CDCl.sub.3) δ 7.25-7.09 (m, 10H), 4.58 (q, J=7.6 Hz, 1H), 4.30 (d, J=10.3 Hz, 2H), 3.30 (dd, J=13.5, 6.9 Hz, 1H), 3.21-3.00 (m, 6H), 2.93-2.83 (m, 1H), 2.36-2.22 (m, 2H), 2.10 (dtt, J=35.8, 14.2, 7.6 Hz, 4H), 1.86-1.67 (m, 2H), 1.49-1.14 (m, 37H). ESI-MS, (m/z): [M+H].sup.+, 896.

Step 3-5: Preparation of tri-tert-butyl (16R,34S)-13,16-dibenzyl-2,2-dimethyl-4,11,14,17,24,32-hexaoxo-3-oxa-5,12,15,18,25,31,33-heptaazahexatriacontane-30,34,36-tricarboxylate (10)

[0112] A solution of the compound 9 (150 mg, 0.2 mmol) was mixed with Boc-6-Ahx-OH (42 mg, 0.2 mmol) and DIPEA (0.1 mL, 0.6 mmol), dissolved in DMF (2 mL), and then cooled to 0° C. HBTU (127 mg, 1.4 mmol) in DMF (1 mL) was added dropwise thereto, and then the reaction mixture was stirred at room temperature for 18 hours. Upon completion of the reaction, ethyl acetate (15 mL) was added thereto, and the organic layer was washed with water (3×20 mL), dried over Na.sub.2SO.sub.4 and concentrated under reduced pressure. The mixture was purified by silica gel column chromatography using DCM and MeOH (96:3, v/v) to give the product 10 as a white solid (155 mg, 70%).

[0113] .sup.1H NMR (500 MHz, CDCl.sub.3) δ 7.26-7.10 (m, 10H), 4.74-0.58 (m, 3H), 4.45 (q, J=6.5 Hz, 2H), 3.25-2.82 (m, 9H), 2.39-2.15 (m, 5H), 2.09 (dt, J=13.0, 6.5 Hz, 4H), 1.92-1.71 (m, 2H), 1.68-1.30 (m, 52H). ESI-MS, (m/z): [M+H].sup.+, 1109.

Step 3-6: Preparation of (3S,21R)-31-amino-21,24-dibenzyl-5,13,20,23,26-pentaoxo-4,6,12,19,22,25-hexaazatriacontane-1,3,7-tricarboxylic Acid (11)

[0114] A solution of the compound 10 (50 mg, 0.05) was dissolved in a mixture of TFA and DCM (2 mL, 1:1, v/v), and stirred at room temperature overnight. Upon completion of the reaction, the solvent was removed under reduced pressure, and the residue was purified by HPLC (method 4) to give the product 11 as a white solid (17 mg, 44%).

[0115] .sup.1H NMR (500 MHz, Methanol-d4) δ 7.34-7.11 (m, 10H), 4.69-4.47 (m, 2H), 4.30 (ddd, J=14.8, 8.4, 4.9 Hz, 2H), 3.21-3.13 (m, 2H), 3.08 (ddt, J=13.4, 6.8, 2.9 Hz, 4H), 2.99-2.74 (m, 4H), 2.41 (ddd, J=8.2, 6.9, 1.9 Hz, 2H), 2.21-2.08 (m, 4H), 1.96-1.77 (m, 2H), 1.61 (s, 2H), 1.58-1.12 (m, 16H). ESI-MS, (m/z): [M+H].sup.+, 841.

Step 3-7: Preparation of (3S,21R)-21,24-dibenzyl-35-isocyano-5,13,20,23,26,33-hexaoxo-4,6,12,19,22,25,32-heptaazapentatriacontane-1,3,7-tricarboxylic Acid (16)

[0116] DIPEA (2.5 eq) was added to a solution of the compound 11 (35 mg, 0.039 mmol) in anhydrous methanol (1 mL), followed by stirring at room temperature for 10 minutes. A solution of the compound 14 (14 mg, 0.05 mmol) in methanol (1 mL) was added thereto and stirred for 5 hours. Upon completion of the reaction, the solvent was removed using a vacuum concentrator and purified by HPLC (method 6) to give the product 16 as a white solid (14 mg, 40%).

[0117] .sup.1H NMR (500 MHz, Methanol-d4) δ 7.22 (dq, J=14.6, 7.0 Hz, 10H), 4.56 (ddd, J=14.1, 8.9, 6.0 Hz, 2H), 4.24 (td, J=7.7, 4.8 Hz, 2H), 3.25-2.88 (m, 12H), 2.54 (s, 2H), 2.38 (ddd, J=8.7, 6.6, 2.7 Hz, 2H), 2.17-2.06 (m, 4H), 1.59 (ddd, J=30.6, 14.5, 7.2 Hz, 4H), 1.46-1.14 (m, 16H). ESI-MS, (m/z): [M+H]+, 921.

<Example 3> Preparation of [.SUP.99m.Tc]Tc-15, the Compound 15 of Example 1 Labeled with .SUP.99m.Tc

[0118] [.sup.99mTc]Tc-15 was prepared as follows according to reaction formula 2 above.

[0119] A [.sup.99mTc][Tc(H.sub.2O).sub.3(CO).sub.3] precursor was prepared using an IsoLink kit. A kit comprising sodium tetraborate decahydrate (2.9 mg), sodium carbonate (7.8 mg), potassium sodium tartrate tetrahydrate (9.0 mg) and disodium borenocarbonate (4.5 mg) was added to .sup.99mTcO.sub.4— (1 mL, 555-740 MBq). The vial was heated in a heating block at 100° C. for 30 minutes and equilibrated for 10 minutes at room temperature. 1 N HCl (200 μL) was added thereto to adjust the pH to 6-6.5. Radiochemical purity was determined by radio-HPLC. The prepared [.sup.99mTc][Tc(H.sub.2O).sub.3(CO).sub.3].sup.+ (500 μL, 370 MBq) solution was added to a vial containing a mixture of methanol and water (100 μg, 200 μL, 3:1 v/v) and compound 15. The vial was heated at 100° C. for 30 minutes and purified by radio-HPLC. The eluted fractions containing the .sup.99mTc-labeled conjugate were collected, diluted with water (20 mL) and then passed through a Sep-Pak C18 cartridge of ethanol (12 mL) and water (12 mL). The Sep-Pak C18 cartridge was washed with water (5 mL), and the .sup.99mTc labeled conjugate was eluted with ethanol (1 mL). Then, the eluent was diluted with saline for further in vitro and imaging studies.

<Example 4> Preparation of [.SUP.99m.Tc]Tc-16, the Compound 16 of Example 2 Labeled with .SUP.99m.Tc

[0120] [.sup.99mTc]Tc-16 was prepared as follows according to reaction formula 2 above.

[0121] A [.sup.99mTc][Tc(H.sub.2O).sub.3(CO).sub.3] precursor was prepared using an IsoLink kit. A kit comprising sodium tetraborate decahydrate (2.9 mg), sodium carbonate (7.8 mg), potassium sodium tartrate tetrahydrate (9.0 mg) and disodium borenocarbonate (4.5 mg) was added to .sup.99mTcO.sub.4— (1 mL, 555-740 MBq). The vial was heated in a heating block at 100° C. for 30 minutes and equilibrated for 10 minutes at room temperature. 1 N HCl (200 μL) was added thereto to adjust the pH to 6-6.5. Radiochemical purity was determined by radio-HPLC. The prepared [.sup.99mTc][Tc(H.sub.2O).sub.3(CO).sub.3].sup.+ (500 μL, 370 MBq) solution was added to a vial containing a mixture of methanol and water (100 μg, 200 μL, 3:1 v/v) and compound 16. The vial was heated at 100° C. for 30 minutes and purified by radio-HPLC. The eluted fractions containing the .sup.99mTc-labeled conjugate were collected, diluted with water (20 mL) and then passed through a Sep-Pak C18 cartridge of ethanol (12 mL) and water (12 mL). The Sep-Pak C18 cartridge was washed with water (5 mL), and the .sup.99mTc labeled conjugate was eluted with ethanol (1 mL). Then, the eluent was diluted with saline for further in vitro and imaging studies.

<Example 5> Preparation of Re-15, a Rhenium Complex of the Compound 15 of Example 1

[0122] Re-15 was prepared according to reaction formula 3.

[0123] Re(CO).sub.3(H.sub.2O).sub.3]Br, a rhenium precursor, was prepared by the informed method. Bromopentacarbonyl rhenium (I) (100 mg, 0.25 mmol) was heated in deionized water (5 mL) for 24 hours to obtain [Re(CO).sub.3(H.sub.2O).sub.3]Br at a concentration of 2 mg/mL. For the preparation of Re-15, [Re(CO).sub.3(H.sub.2O).sub.3]Br (400 μL, 1 μmol) was added to the precursor 15 (11 mg, 0.02 mmol) in methanol (2 mL) and then heated at 1000 for 4 hours. The reaction mixture was heated at 100° C. for 3 hours. The resulting mixture was concentrated using a rotary evaporator and purified by HPLC.

<Example 6> Preparation of Re-16, a Rhenium Complex of the Compound 16 of Example 2

[0124] Re-16 was prepared according to reaction formula 3.

[0125] Re(CO).sub.3(H.sub.2O).sub.3]Br, a rhenium precursor, was prepared by the informed method Bromopentacarbonyl rhenium (I) (100 mg, 0.25 mmol) was heated in deionized water (5 mL) for 24 hours to obtain [Re(CO).sub.3(H.sub.2O).sub.3]Br at a concentration of 2 mg/mL. For the preparation of Re-16, [Re(CO).sub.3(H.sub.2O).sub.3]Br (400 μL, 1 μmol) was added to the precursor 16 (10 mg, 0.01 mmol) in a mixture of methanol (2 mL) and water (3 mL, 1:1 v/v). The reaction mixture was heated at 100° C. for 2 hours. The resulting mixture was concentrated using a rotary evaporator and purified by HPLC.

<Experimental Example 1> Stability Test in Human Serum

[0126] The stability test in human serum is to test the stability when it comes into contact with human serum after administration to the human body, and the stability in the body is partly tested in vitro.

[0127] 1. [.sup.99mTc]Tc-15 According to the Present Invention

[0128] In order to examine the stability of [.sup.99mTc]Tc-15 according to the present invention in human serum, 3.7 MBq of [.sup.99mTc]Tc-15 (100 μL) prepared in Example 3 was added to 1 mL of human serum, mixed well, and then cultured at 36.5° C. with stirring. After 2 hours, the reaction mixture was analyzed by ITLC.

[0129] As a result, it was confirmed that most of them were present as [.sup.99mTc]Tc-15 and hardly separated as .sup.99mTc (FIG. 2A).

[0130] 2. [.sup.99mTc]Tc-16 According to the Present Invention

[0131] In order to examine the stability of [.sup.99mTc]Tc-16 according to the present invention in human serum, 3.7 MBq of [.sup.99mTc]Tc-16 (100 μL) prepared in Example 4 was added to 1 mL of human serum, mixed well, and then cultured at 36.5° C. with stirring. After 2 hours, the reaction mixture was analyzed by ITLC.

[0132] As a result, it was confirmed that most of them were present as [.sup.99mTc]Tc-16 and hardly separated as .sup.99mTc (FIG. 2B).

[0133] Therefore, [.sup.99mTc]Tc-15 or [.sup.99mTc]Tc-16 according to the present invention is stable enough to obtain images because technetium, a soft acid, and isonitrile, a soft base, bind very strongly and have excellent stability in human serum.

<Experimental Example 2> In Vitro Cell Binding Test

[0134] In order to conduct the in vitro cell binding test of [.sup.99mTc]Tc-15 and [.sup.99mTc]Tc-16 according to the present invention, the following experiment was performed.

[0135] The PSMA-positive prostate cancer cell line 22Rv1 was placed in a 24-well plate at the density of 1×10.sup.5 cells/well, followed by culture in a 37° C., 5% CO.sub.2 incubator for 24 hours. Each of [.sup.99mTc]Tc-15 and [.sup.99mTc]Tc-16 prepared in Examples 3 and 4 were diluted with a cell culture medium containing 0.5% bovine serum albumin by sequential two-fold dilution. 0.5 mL of the diluted solution was taken and put into cultured cells and incubated in a 37° C., 5% CO.sub.2 incubator for 1 hour. After removing the culture medium, the cells were washed twice with fresh culture medium, 1 mL of 0.5% sodium dodecyl sulfate (SDS) dissolved in phosphate buffered saline was added thereto, gently shaken to dissolve, and then transferred to a 5 mL disposable plastic test tube. The radioactivity of the tube was measured using a gamma counter. When [.sup.99mTc]Tc-15 and [.sup.99mTc]Tc-16 were added to measure non-specific binding, 2-(phosphonomethyl)-pentanedonic acid (PMPA) was added at a concentration of 250 μM and the same experiment was performed. Specific binding was obtained by subtracting non-specific binding from total binding, and a saturation curve was drawn. FIGS. 3A and 3B are the saturation curves for [.sup.99mTc]Tc-15 and [.sup.99mTc]Tc-16, respectively. From these saturation curves, the dissociation constant, Kd, was calculated by nonlinear regression using GraphPad Prism 7 program. As a result, the Kd values of [.sup.99mTc]Tc-15 and [.sup.99mTc]Tc-16 according to the present invention were 5.5 nM and 0.2 nM, respectively, and both showed sufficient binding force for imaging and treatment, but [.sup.99mTc]Tc-16 had a higher binding force among the two.

<Experimental Example 3> PET Image Verification in Cancer Transplanted Experimental Animals

[0136] [.sup.99mTc]Tc-15 and [.sup.99mTc]Tc-16 according to the present invention were administered to animals transplanted with prostate cancer and the following experiment was performed to verify cancer tissue targeting through PET imaging.

[0137] Specifically, 0.1 mL of RPMI1640 culture medium containing 5×10.sup.6 22 Rv1 cells was subcutaneously injected into the right back of a 4-week-old male BALE/c nude mouse. After 2 to 3 weeks, the present inventors confirmed that the tumor tissue had reached an appropriate size and used it in the experiment. For animal injection, [.sup.99mTc]Tc-15 and [.sup.99mTc]Tc-16 were each diluted in saline for injection to a concentration of 10.2 MBq/100 μL. Each of these was injected into the tail vein of the mice transplanted with cancer cells, and after 1 hour and 3 hours, SPECT for animals was taken for 10 minutes.

[0138] As a result, it was observed that [.sup.99mTc]Tc-15 and [.sup.99mTc]Tc-16 according to the present invention were not only excreted by the kidney but also ingested into the prostate cancer tissue. In particular, [.sup.99mTc]Tc-16 showed a higher intake in prostate cancer (FIGS. 4A and 5A). The results show that the blood concentration of [.sup.99mTc]Tc-16, which binds to albumin, was maintained higher, and thus the intake into prostate cancer was increased.

[0139] In addition, when MIP-1072 that binds to PSMA was previously injected prior to the administration of [.sup.99mTc]Tc-15 and [.sup.99mTc]Tc-16, tumor intake was not observed (FIGS. 4B and 5B). This proves that the prostate cancer images of [.sup.99mTc]Tc-15 and [.sup.99mTc]Tc-16 according to the present invention are shown by specific binding to PSMA.

[0140] Therefore, since [.sup.99mTc]Tc-15 and [.sup.99mTc]Tc-16 according to the present invention bind specifically to PSMA, they can be effectively used for imaging of prostate cancer, and it can be seen that [.sup.99mTc]Tc-16, which has an albumin binding site in the linker, shows a better image.

<Experimental Example 4> In Vivo Distribution Test in Cancer Transplantation Animals

[0141] [.sup.99mTc]Tc-15 or [.sup.99mTc]Tc-16 according to the present invention was administered to an animal transplanted with prostate cancer, and it was verified whether it was actually observed in cancer tissue by in vivo distribution test.

[0142] Specifically, 0.1 mL of RPMI1640 culture medium containing 5×10.sup.6 22 Rv1 cells was subcutaneously injected into the left flank of a 4-week-old male BALB/c nude mouse. After 2 to 3 weeks, the present inventors confirmed that the tumor tissue had reached an appropriate size and used it in the experiment. For animal injection, [.sup.99mTc]Tc-15 prepared in Example 3 and [.sup.99mTc]Tc-16 prepared in Example 4 were each diluted in saline for injection to a concentration of 0.74 MBq/100 μL. Each of these was injected into the tail vein of the mice transplanted with cancer cells, and after 1 hour and 4 hours, cancer, blood, muscle, heart. Lung, liver, spleen, stomach, small intestine, kidney, bone, and the like were collected and their weight and radioactivity were measured. Based on this data, the intake amount for each unit tissue (% ID/g) was calculated and shown in Tables 2 and 3.

TABLE-US-00002 TABLE 2 In vivo distribution and absorption rate of [.sup.99mTc]TC-15 in PSMA-targeted 22Rv1 tumor bearing BALB/c male nude mice at 1 and 4 hours Tissues 1 h 4 h 1 h Blockade 4 h Blockade Blood 0.60 ± 0.02 0.31 ± 0.02 0.52 ± 0.09 0.31 ± 0.01 Muscle 0.17 ± 0.07 0.08 ± 0.01 0.11 ± 0.02 Tumor   1.48 ± 0.18***  0.81 ± 0.09 0.38 ± 0.12 0.20 ± 0.04 Heart 0.22 ± 0.02 0.12 ± 0.01 0.10 ± 0.01 Lung 0.64 ± 0.02 0.30 ± 0.04 0.30 ± 0.05 Liver 0.94 ± 0.05 0.85 ± 0.05 0.84 ± 0.12 Spleen 0.90 ± 0.14 0.26 ± 0.06 0.15 ± 0.03 0.16 ± 0.01 Stomach 0.31 ± 0.07 0.15 ± 0.02 0.17 ± 0.04 0.14 ± 0.01 Intestine 0.81 ± 0.19 0.97 ± 0.20 Kidney  59.69 ± 8.45*** 4.22 ± 1.30 Bone 0.34 ± 0.02 0.16 ± 0.03 0.20 ± 0.03 Tumor/blood  2.5 ± 0.18  2.6 ± 0.13 Tumor/muscle 10.4 ± 0.77  3.5 ± 0.50 2.71 ± 0.78 Tumor/liver 1.57 ± 0.15 0.96 ± 0.13 0.45 ± 0.10 0.24 ± 0.04 Tumor/kidney 0.02 ± 0.00 0.06 ± 0.02 0.09 ± 0.02 0.14 ± 0.03 Results were expressed % ID/g (mean ± SD for n = 3). Blocking was performed by co-injection of 2-PMPA (100 μg). ***p ≤ 0.001, **p ≤ 0.01. indicates data missing or illegible when filed

TABLE-US-00003 TABLE 3 In vivo distribution and absorption rate of [.sup.99mTc]Tc-16 in PSMA-targeted in 22Rv1 tumor bearing BALB/c male nude mice at 1 and 4 hours Tissues 1 h 4 h 1 h Blockade 4 h Blockade Blood 0.42 ± 0.01 0.17 ± 0.03 0.76 ± 0.08 0.15 ± 0.01 Muscle 0.13 ± 0.02 0.07 ± 0.01 0.22 ± 0.06 0.05 ± 0.01 Tumor    2.83 ± 0.26 *** 0.45 ± 0.02 0.39 ± 0.03 Heart 0.36 ± 0.01 0.25 ± 0.05 0.48 ± 0.05 0.11 ± 0.01 Lung 0.52 ± 0.05 0.36 ± 0.04 1.63 ± 0.10 0.45 ± 0.04 Liver 1.17 ± 0.04 0.69 ± 0.11 1.95 ± 0.07 1.33 ± 0.15 Spleen 3.40 ± 0.93 0.40 ± 0.03 0.20 ± 0.04 Stomach 0.85 ± 0.23 0.42 ± 0.04 1.04 ± 0.26 0.23 ± 0.04 Intestines 1.48 ± 0.16 1.26 ± 0.14 Kidney .sup. 24.66 ± 2.17 ** Bone 0.63 ± 0.03 1.16 ± 0.08 0.47 ± 0.12 Tall 0.49 ± 0.10 0.48 ± 0.12 1.18 ± 0.14 Tumor/blood 4.43 ± 0.39 2.67 ± 0.25 Tumor/muscle 14.05 ± 1.78  40.43 ± 3.97  2.16 ± 0.43 Tumor/liver 1.60 ± 0.13 4.17 ± 0.36 0.23 ± 0.02 0.30 ± 0.04 Tumor/kidney 0.08 ± 0.01 0.07 ± 0.01 0.08 ± 0.01 0.11 ± 0.01 Results were expressed as % ID/g (mean ± SD for n = 4). Blocking was performed by co-injection of 2-PMPA (100 μg). *** p ≤ 0.001, ** p ≤ 0.01. indicates data missing or illegible when filed

[0143] As shown in Tables 2 and 3, [.sup.99mTc]Tc-15 or [.sup.99mTc]Tc-1.6 according to the present invention showed the highest intake in the kidney like a conventional radiopharmaceutical for peptide imaging, followed by a high intake in the cancer tissue. However, the intake of Tc-15 in the liver and the intake of Tc-16 in the spleen appeared to be slightly higher than the intake in the cancer tissue, but none of them were statistically significant. However, the intake of [.sup.99mTc]Tc-15 in the liver and the intake of [.sup.99mTc]Tc-16 in the spleen appeared to be slightly higher than the intake in the cancer tissue, but none of them were statistically significant. In addition, as both [.sup.99mTc]Tc-1.5 and [.sup.99mTc]Tc-16 showed that the intake was decreased when PSMA was blocked with PMPA, it was found that the intake into cancer cells was specific to PSMA.

[0144] Therefore, since [.sup.99mTc]Tc-15 or [.sup.99mTc]Tc-16 according to the present invention is effective for imaging of prostate cancer, it can be effectively used as a radiopharmaceutical, and it can be seen that [.sup.99mTc]Tc-16 having an albumin binding site in the linker is superior.

[0145] As mentioned above, the present invention has been described in detail through the preferred preparative examples, examples and experimental examples, but the scope of the present invention is not limited to the specific examples, and should be interpreted by the appended claims. In addition, those of ordinary skill in the art should understand that many modifications and variations are possible without departing from the scope of the present invention.

INDUSTRIAL APPLICABILITY

[0146] The GUL-isonitrile derivative and radioactive metal complex according to the present invention is simple to label, has high labeling efficiency, has excellent stability in human serum when administered to the body, binds well to PSMA expressed in prostate cancer, and has high water solubility, so it is excreted by the kidney rather than the hepatobiliary tract, so the intake is very small in the intestines. In addition, the GUL-isonitrile derivative and radioactive metal complex of the present invention accumulates in prostate cancer tissue and emits radiation from the prostate cancer tumor site, so it can be effectively used as a therapeutic or diagnostic pharmaceutical composition.