Device and method for skin laser treatment

Abstract

A system for treating a region of the epidermis, including: at least one laser energy source; a time control device to generate a laser beam; a laser energy focusing system arranged and produced to direct a laser beam on the region of the epidermis. The control device generates a laser beam including a plurality of composite pulses, emitted at a base frequency, each composite pulse including a sequence of sub-pulses at a higher frequency than the base frequency.

Claims

1. A method for treating a portion of epidermis of a patient, the method comprising: emitting a laser beam comprising one or more composite pulses, emitted at a base frequency, each of said one or more composite pulses comprising a sequence of sub-pulses at a higher frequency than said base frequency, each of said composite pulses comprising a first interval of continuous pre-pulse emission and a second sub-pulse sequence emission interval, said sub-pulse sequence comprising a plurality of sub-pulses, said sub-pulse sequence emission interval comprising a train of sub-pulses following said pre-pulse emission, said first interval of continuous pre-pulse emission comprising a first interval pre-pulse emission duration, each of said subsequent sub-pulses having a sub-pulse duration, said first interval pre-pulse emission duration being greater than said sub-pulse duration of each of said sub-pulses.

2. A method according to claim 1, wherein said pre-pulse emission has a higher energy per surface unit than said sub-pulses.

3. A method according to claim 1, wherein said pre-pulse emission has a higher peak power than said sub-pulses.

4. A method according to claim 1, further comprising the step of combining a radiofrequency current with said laser beam.

5. A method according to claim 4, wherein said radiofrequency current is coordinated in time with the laser beam so that the laser beam and the radiofrequency current are emitted in manner overlapped in time and/or with the radiofrequency current emitted in succession to the laser beam.

6. A method for treating a portion of epidermis of a patient, the method comprising: emitting a pulsed laser beam toward said portion of epidermis, said pulsed laser beam comprising a plurality of composite pulses emitted at a base frequency, each of said composite pulses comprising a sequence of sub-pulses, said sequence of sub-pulses being emitted at a higher frequency than said base frequency, each of said composite pulses comprising a first interval of continuous pre-pulse emission followed by a sub-pulse sequence emission interval, said sub-pulse sequence emission interval comprising a plurality of sub-pulses following said pre-pulse emission, said first interval of continuous pre-pulse emission having a greater duration than each of said subsequent sub-pulses; emitting, in combination in time with said laser beam, a radiofrequency current toward said portion of epidermis.

7. A method for emitting energy through a handpiece, the method comprising the steps of: providing a device with a handpiece; emitting a pulsed laser beam and a radiofrequency current coordinated in time with each other, said pulsed laser beam comprising a plurality of composite pulses emitted at a base frequency, each of said composite pulses comprising a continuous pre-pulse emission interval followed by a sub-pulse sequence emission interval, said continuous pre-pulse emission interval comprising a continuous pre-pulse emission, said sub-pulse sequence emission interval comprising a plurality of sub-pulses following said pre-pulse emission, said continuous pre-pulse emission interval comprising a pre-pulse emission interval duration, each of said sub-pulses comprising a sub-pulse duration, said pre-pulse emission interval duration being greater than said sub-pulse duration of each of said sub-pulses, said plurality of sub-pulses being emitted at a sub-pulse frequency, said sub-pulse frequency being greater than said base frequency; transmitting energy through the handpiece.

8. A method according to claim 7, wherein said radiofrequency current is emitted in a time interval at least partly overlapped and/or subsequent to an emission interval of the pulsed laser beam.

9. A system for treating a region of the epidermis, the system comprising: at least one laser energy source to generate a pulsed laser beam having a laser-ablation effect; a laser energy focusing system arranged and designed to direct a laser beam on said region of the epidermis; a radiofrequency current source with at least one electrode for applying radiofrequency current; a control configured to emit said laser beam and said radiofrequency current in a timely-coordinated manner such that said laser beam comprises a plurality of composite pulses emitted at a base frequency, each of said composite pulses comprising a continuous pre-pulse emission interval followed by a sub-pulse sequence emission interval, said continuous pre-pulse emission interval comprising a continuous pre-pulse emission, said sub-pulse sequence emission interval comprising a plurality of sub-pulses following said pre-pulse emission, said continuous pre-pulse emission interval comprising a pre-pulse emission interval duration, each of said sub-pulses comprising a sub-pulse duration, said pre-pulse emission interval duration being greater than said sub-pulse duration of each of said sub-pulses, said plurality of sub-pulses being emitted at a sub-pulse frequency, said sub-pulse frequency being greater than said base frequency.

10. A system according to claim 9, wherein said control is designed to emit said radiofrequency current at least partly simultaneously to, and/or in sequence with, application of said laser beam.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

(1) In the drawings:

(2) FIG. 1 is a view of a device embodying the invention;

(3) FIG. 2 is a detailed view of a handpiece of the device of FIG. 1;

(4) FIG. 3 is a view of a diagram of a laser-beam scanning system;

(5) FIG. 4 is a view of a system for dividing a main laser beam in a plurality of adjacent or contiguous laser beams;

(6) FIG. 5 is a view of a matrix according to which the laser treatment points of a portion of epidermis can be arranged;

(7) FIG. 6 is a schematic view showing an improved handpiece for combined laser and radio frequency treatment;

(8) FIG. 6A is a schematic view of an improved embodiment of an electrode for applying radio frequency current;

(9) FIG. 6B is a schematic view of an improved embodiment of an electrode for applying radio frequency current;

(10) FIG. 6C is a schematic view of an improved embodiment of an electrode for applying radio frequency current;

(11) FIG. 6D is a schematic view of an improved embodiment of an electrode for applying radio frequency current;

(12) FIG. 7 is a view of the handpiece of FIG. 6;

(13) FIG. 8 is a view showing the shape of the laser pulse in one embodiment;

(14) FIG. 9 is a view showing the shape of the laser pulse in another embodiment;

(15) FIG. 10A is a view of a histological image of tissue treated with two different types of laser pulses according to the invention in different application conditions;

(16) FIG. 10B is a view of a histological image of tissue treated with two different types of laser pulses according to the invention in different application conditions;

(17) FIG. 10C is a view of a histological image of tissue treated with two different types of laser pulses according to the invention in different application conditions;

(18) FIG. 10D is a view of a histological image of tissue treated with two different types of laser pulses according to the invention in different application conditions;

(19) FIG. 10E is a view of a histological image of tissue treated with two different types of laser pulses according to the invention in different application conditions;

(20) FIG. 10F is a view of a histological image of tissue treated with two different types of laser pulses according to the invention in different application conditions;

(21) FIG. 10G is a view of a histological image of tissue treated with two different types of laser pulses according to the invention in different application conditions;

(22) FIG. 10H is a view of a histological image of tissue treated with two different types of laser pulses according to the invention in different application conditions;

(23) FIG. 10I is a view of a histological image of tissue treated with two different types of laser pulses according to the invention in different application conditions;

(24) FIG. 10J is a view of a histological image of tissue treated with two different types of laser pulses according to the invention in different application conditions;

(25) FIG. 10K is a view of a histological image of tissue treated with two different types of laser pulses according to the invention in different application conditions;

(26) FIG. 11A is a schematic view showing the effect of ablation and of thermal shock in the tissue treated with different types of laser pulses according to the invention;

(27) FIG. 11B is a schematic view showing the effect of ablation and of thermal shock in the tissue treated with different types of laser pulses according to the invention;

(28) FIG. 12A is a schematic view showing the effect of ablation and of thermal shock in the tissue treated with different types of laser pulses according to the invention;

(29) FIG. 12B is a schematic view showing the effect of ablation and of thermal shock in the tissue treated with different types of laser pulses according to the invention;

(30) FIG. 12C is a schematic view showing the effect of ablation and of thermal shock in the tissue treated with different types of laser pulses according to the invention;

(31) FIG. 13 is a view of a diagram of the conductivity of the tissue as a function of the frequency of a radio frequency electrical current;

(32) FIG. 14A is a view of a diagram of the trend over time of hemoglobin in a tissue treated with laser pulses according to the invention with and without application of radiofrequency electrical current;

(33) FIG. 14B is a view of a diagram of the trend over time of hemoglobin in a tissue treated with laser pulses according to the invention with and without application of radiofrequency electrical current;

(34) FIG. 14C is a view of a diagram of the trend over time of hemoglobin in a tissue treated with laser pulses according to the invention with and without application of radiofrequency electrical current;

(35) FIG. 14D is a view of a diagram of the trend over time of hemoglobin in a tissue treated with laser pulses according to the invention with and without application of radiofrequency electrical current;

(36) FIG. 14E is a view of a diagram of the trend over time of hemoglobin in a tissue treated with laser pulses according to the invention with and without application of radiofrequency electrical current;

(37) FIG. 15 is a view of a diagram illustrating the shrinkage effect provoked by the different types of treatment;

(38) FIG. 16 is a view of a diagram illustrating the speed of disappearance of skin reddening caused by the treatment in various treatment conditions;

(39) FIG. 17 is a view of a diagram relating to the formation of plasma as a function of the density of emitted laser energy; and

(40) FIG. 18 is a view of a time diagram explaining the biological phenomena provoked by the combined application of optical energy in the form of laser radiation and electrical energy in the form of radio frequency current.

DESCRIPTION OF THE PREFERRED EMBODIMENTS

(41) Structure of the Handpiece and of the Optics

(42) The following detailed description of exemplary embodiments refers to the accompanying drawings. The same reference numbers in different drawings identify identical or similar elements. Moreover, the drawings are not necessarily in scale. Further, the following detailed description does not limit the invention. Rather, the scope of the invention is defined by the appended claims.

(43) Reference in the whole of the description to an embodiment or the embodiment or some embodiments means that a particular feature, structure or element described in relation to an embodiment is included in at least one embodiment of the subject described. Therefore, the phrase in an embodiment or in the embodiment or in some embodiments in various points throughout the description does not necessarily refer to the same embodiment or embodiments. Moreover, the particular features, structures or elements can be combined in any suitable manner in one or more embodiments.

(44) FIGS. 1 and 2 show a device in which the invention can be incorporated. In general, the device 1 comprises a base 3, wherein at least one laser source 5 is housed. The laser source 5 can be a continuous laser, but preferably a pulsed laser is used. The block indicated generically with 5 is intended also as including a system to control the emission in time of the laser radiation, i.e. the pulse generation system.

(45) According to some embodiments, the laser source can have an emitting wavelength comprised between 532 and 13,000 nm and more in particular a wavelength of 10600 nm, corresponding to CO.sub.2 laser emission. In fact, the laser source is preferably a CO.sub.2 laser.

(46) In some modes of use, the laser can be controlled so as to provide a pulse for each position or point of a treatment pattern. However, in other modes of use more than one laser pulse can be fired for each operating position, i.e. at each point treated. For example, from two to five pulses can be provided for each position of the laser. Preferably, the laser is controlled so as to be able to emit one or more pulses for each position or point of the pattern on the portion of epidermis to be treated, depending on the settings chosen by the operator. Movement of the laser beam can be obtained through a system of scanning mirrors described in greater detail below. Preferably, the laser emission is interrupted when moving from one treatment position to the other, i.e. from one point to the other of a treatment pattern.

(47) Advantageously, in some embodiments the laser beam has a Gaussian power distribution, with a maximum power density at the center and decreasing toward the periphery of the cross section of the beam. To obtain the Gaussian shape of the beam, in some embodiments the laser cavity is produced so as to isolate the fundamental propagation mode and the focusing optics must be designed to contribute to maintaining the Gaussian shape of the energy distribution when moving from the axis outwardly. An appropriate choice of cavity diameter and an appropriate radius of the mirrors of the laser source are able to provide generation of the TEM00 oscillation mode that provides a Gaussian beam profile.

(48) The laser beam can be conveyed through a waveguide 7 toward a handpiece 9. The guide can be designed in various ways, also depending upon the frequency and the emission power of the laser. In the example illustrated the waveguide is simply made of hollow tubular elements, joined to one another and inside which mirrors for deflecting the laser beam are arranged to deviate the beam along the axis of the various tubular portions of the guide.

(49) Inside the handpiece 9 there are arranged focusing systems and/or scanning of the laser beam, some of which are represented schematically in FIGS. 3 and 4. Preferably in the handpiece 9 there is contained a scanning system (FIG. 3) comprising for example two scanning mirrors 21 with related actuators 23 controlled electronically by a control unit, not shown. The scanning mirrors control the movement of the laser beam F output from the handpiece 13, so that it follows a given path, according to criteria better defined below. Therefore, in this case a single laser beam F is outputted from the handpiece and directed toward the surface of the epidermis to be treated, from which the handpiece can be held at a constant distance, for example by means of a spacer 11. On the handpiece 13 push buttons, knobs or other adjustment and interface members can be arranged, schematically indicated with 15, through which the operator can modify the shape of the beam and/or the dimension and the area of the scanning surface, the movement of the beam and the like.

(50) Through the handpiece 13 and the scanning system contained therein it is possible to control movement of the beam F according to a defined and stored pattern, optionally modifiable by the user.

(51) In a suitable position along the path of the laser beam a focusing optic is arranged. In the diagram of FIG. 3 said optic is indicated with 25 and is placed in the handpiece, but it must be understood that this is not strictly necessary and that other positions are possible. The optic 25 also has the function of imposing on the beam a given energy density distribution as a function of the radius, as will be clarified below.

(52) In other embodiments, inside the handpiece 13 there are arranged focusing systems which divide the laser beam into a plurality of beams adjacent to one another and which impart to each of the adjacent beams an energy density profile as a function of the radius according to the criteria described below.

(53) In some embodiments the lens placed in the handpiece in combination with the shape of the beam generated by the source give rise to a Gaussian energy density distribution profile. The shape of the beam generated depends on the purity of the propagation mode inside the laser cavity which consequently determines the energy distribution transverse to the axis of propagation in the free space at the output of the laser source.

(54) In some embodiments the beams with which the portion of epidermis to be treated is irradiated can be adjacent beams generated with an optical system of the type represented in FIG. 4, or can simply be represented by positions assumed in time sequence by a same laser beam which is moved by a scanning system as represented in FIG. 3. In this latter case, the laser beam is preferably switched on, i.e. activated sequentially in each position desired according to a radiation pattern, while during movement between one point and the other the laser is preferably switched off.

(55) Whatever the system for generating the adjacent laser beams, the epidermis can be irradiated, for example, following a pattern with a matrix of points, as indicated schematically in FIG. 5. The letter E generically indicates a treated portion of epidermis and the letter F the points of intersection between the axis of the laser beam and the surface of epidermis being treated. It must be observed that in this case the treatment pattern is formed by a plurality of points arranged according to a matrix or grid with rectangular mesh, the vertices of which form the points in which the center of the laser beam. is positioned. One or more laser pulses can be emitted in each position represented by a point F.

(56) It must be understood that the pattern of FIG. 5 is provided purely by way of example and that different patterns can be used, for example according to a matrix with rhomboidal mesh, or also a pattern in which the points F are arranged according to curved lines, according to a spiral or in any other way. Currently, a pattern according to a matrix with quadrangular, i.e. rectangular or rhomboidal, mesh is preferred.

(57) The shape of the laser pulses used and the values of the emission parameters, and the results obtained with various shapes of laser radiation will be discussed below.

(58) According to improved embodiments of the invention, the laser treatment is combined with a treatment through applying radio frequency. FIGS. 6 and 7 illustrate this embodiment. FIG. 6 shows a handpiece 109, which contains the same components as the handpiece 9, in addition to a radio frequency generator, indicated schematically with 110. The radio frequency generator is connected to a pair of electrodes 113. In some embodiments the electrodes 113 are shaped to form a spacer between the handpiece 109 and the surface to be treated. The distance is determined on the basis of the optical characteristics of the laser, whose radiation is conveyed to the handpiece 109 through a light guide 115, as in the embodiment described previously. Interface means between the appliance and the user are provided on the handpiece 109, such as one or more push buttons or the like, generically indicated with 117.

(59) Using the electrodes as spacers, an instrument that is particularly compact, inexpensive and easy to use is obtained.

(60) With a handpiece of this type it is possible to synergistically combine the effects of laser and of radio frequency on the treated tissue. When the electrodes 113 are resting on the skin to be treated, for example on the patient's face, as shown in FIG. 7, the radiofrequency field generated by the electrodes propagates into the tissues and generates induced currents, which heat the treated tissue.

(61) FIGS. 6A, 6B, 6C and 6D schematically show an improved embodiment of an electrode for applying radio frequency current which prevents or reduces the risk of generating electrical arcs between the electrode and the epidermis of the subject treated when the electrode is moved away from the skin. In this embodiment a device is provided for switching off the electrical power circuit of the RF current, which opens the circuit and cuts off the electrical power inside a protected zone, preventing electrical discharges from generating on the skin. In particular, the electrode 113 can have ends 113A (FIG. 6D) seated in respective cases 114. The ends 113A form first contacts cooperating with second contacts 118 housed in the respective cases 114. The contacts 113A, 118 form a pair of switches which are closed as a result of compression of respective springs 120, advantageously housed in the cases 114, when the electrode 113 is pressed against the skin. Compression of the springs 120 causes the ends 113A of the electrode 113 to contact the contacts 118, closing the electrical circuit. When the operator moves the handpiece 109 on which the electrodes 113 are placed away from the patient's skin, the springs 120 extend, causing the contacts 113A, 118 to move away from each other and consequent opening of the electrical circuit. Any arcs o discharges remain confined inside the cases 114.

(62) It must be understood that an electrode 113 with one movable end 113A and the other end permanently connected to the electrical circuit could also be used. The elastic effect can also be obtained by means of properties of the material with which the electrode 113 is made, without the need to use an auxiliary spring. For example, the electrode 113 can be made in the form of flat spring, with an advantageously arcuate shape. One end of electrode is fixed and the other forms a movable contact which approaches a fixed contact, enclosed in a protected zone, when the handpiece is pressed onto the skin, closing the electrical circuit.

(63) Alternatively to the use of movable contacts, or in combination therewith, a sponge 116, either made of conducting material or preferably made conductive by impregnating it with a conductive liquid, such as a saline solution, can be associated with the electrode 113. The sponge 116 can be shaped appropriately, for example with a groove, to be reversibly fixed to the electrode 113. The sponge 116 can advantageously be disposable, for reasons of hygiene.

(64) Laser radiation and radio frequency can be combined or overlapped in time in various ways, according to criteria that will be clear from the description set forth below.

(65) The results of the combined application of optical radiation and RF current and some possible explanations of the particular efficacy obtainable with this method will be discussed below.

(66) Time Shape of the Laser Beam

(67) It has been discovered, and is an important element of the present invention, that particular shapes of the pulse of the laser radiation, i.e. particular trends over time of the pulsed laser emission, enable much greater biological effects to be obtained on tissue, compared to prior art systems. It has also been discovered that in some cases the laser pulses shaped according to the invention have a synergistic effect in combination with a radio frequency current. As will be illustrated below, the shapes of the pulses according to the invention enable more efficient treatments and faster healing, especially in skin-tissue rejuvenating and firming treatments.

(68) FIG. 8 shows a first time shape of a series of laser pulses according to the invention, i.e. the trend over time of the laser light emission. In this figure, the abscissa indicates the time and the ordinate indicates the emitted power.

(69) Hereinafter the laser pulse having the shape of FIG. 8 will be indicated as pulse S. Said pulse is in fact a composite pulse, where composite pulse is intended as a pulse that is in turn constituted by the combination of sub-pulses, or hypo-energy pulses, as will be described in detail below.

(70) FIG. 8 shows a sequence of pulses SP with the period T. A period T has an on interval -on and an off interval -off. The sum of the time intervals -on and -off is equal to the period T of the pulse. The relation -on/T is defined as the duty cycle of the composite pulse. The inverse 1/T of the period T of the composite pulse is defined as the frequency of the composite pulse. According to some embodiments, the frequency of the composite pulse, hereinafter also defined as base frequency, is comprised between 1 and 1000 Hz, for example between 1 and 500 Hz. The duty cycle of the composite pulse can be comprised between 1% and 90% and preferably between 2% and 50% and even more preferably between 2% and 40%.

(71) As can be observed in FIG. 8, sub-pulses Si are contained in the interval -on of each composite pulse. In the embodiment of FIG. 8 sub-pulses, all of the same duration, are contained in the interval -on. In some embodiments the sub-pulses Si have a frequency comprised between 1 kHz and 200 kHz. In preferred embodiments the frequency of the sub-pulses is comprised between 1 kHz and 100 kHz and even more preferably between 2 kHz and 50 kHz. In some embodiments the frequency is comprised between 5 and 45 kHz, for example between 8 and 40 kHz.

(72) The duty cycle of the sub-pulses, i.e. the relation between the period of the sub-pulse, indicated with Ts in FIG. 8, and the duration of the on-interval (during which sub-pulses are emitted) is determined as a function of the peak power, of the duration -on of the composite pulse and of the energy per pulse that is required to be emitted at each pulse. In some embodiments the on-duration of the single sub-pulse is comprised between 1 and 50 microseconds and preferably between 2 and 40 microseconds. In some embodiments, the duration of the on-period is between 3 and 25 microseconds. The duty cycle can be comprised between 1 and 90% and preferably between 1 and 50% and even more preferably between 2 and 25%. Typically, the duty cycle is comprised between 3 and 24%.

(73) La peak power, indicated in FIG. 8, can be comprised between 10 and 200 W, preferably between 40 and 190 W.

(74) In some embodiments the energy per pulse of the composite pulses is comprised between 0.2 and 200 mJ, for example between 0.4 and 150 mJ and preferably between 0.4 and 130 mJ

(75) The energy of the single sub-pulse Si can be comprised between 0.2 and 10 mJ and preferably between 0.4 and 8 mJ.

(76) The spot area, i.e. the area of the section of the laser beam on the surface onto which the beam is projected, is advantageously comprised between 0.0001 and 0.0003 cm.sup.2 and preferably between 0.00015 and 0.0002 cm.sup.2. The fluence, i.e. the energy per unit of surface area, is obtained as the ratio between the powers and the spot areas indicated above. According to some embodiments the diameter of the spot is comprised between 50 and 500 micrometers, preferably between 80 and 400 micrometers, even more preferably between 100 and 200 micrometers, for example around 150 micrometers.

(77) The average power can be comprised between 2 and 100 W, for example between 4 and 80 W, preferably between 4 and 50 W.

(78) In some embodiments of the invention the number of pulses Si for each train or composite pulse can be comprised between 1 and 100 and preferably greater than 1 and less than or equal to 80.

(79) The following tables 1 and 2 each indicate two series of values for the main parameters of the pulse. It must be understood that each parameter may vary in the interval defined by the two values of the corresponding line.

(80) TABLE-US-00001 TABLE 1 Repetition frequency (Hz) 10,000 10,000 Duration of the sub-pulse (s) 100 100 On time of the sub-pulse (s) 4 24 Off time of the sub-pulse (s) 96 76 Duty Cycle (%) of the 4% 24% sub-pulse Peak power of the 12 180 sub-pulse (W) Energy of the sub-pulse (mJ) 0.4 6.0 Total energy of the train of 0.4 120.0 pulses (mJ) Number of pulses per train 1 20 (i.e. per composite pulse) Spot diameter (m) 150 150 Spot area (cm.sup.2) 0.0001767146 0.0001767146 Fluence of the single sub- 2.26 33.95 pulse (J/cm.sup.2) Fluence of the composite 2.26354 679.06109 pulse (J/cm.sup.2) Average power (W) 4 60 Dwell time (s) 100 2000

(81) TABLE-US-00002 TABLE 2 Repetition frequency (Hz) 40,000 40,000 Duration of the sub-pulse (s) 25 25 On time of the sub-pulse (s) 1 6 Off time of the sub-pulse (s) 24 19 Duty Cycle (%) of the 4% 24% sub-pulse Peak power of the 6 90 sub-pulse (W) Energy of the sub-pulse (mJ) 0.1 1.5 Total energy of the train of 0.4 120.0 pulses (mJ) Number of pulses per train 4 80 (i.e. per composite pulse) Spot diameter (m) 150 150 Spot area (cm.sup.2) 0.0001767146 0.0001767146 Fluence of the single sub- 0.57 8.49 pulse (J/cm.sup.2) Fluence of the composite 2.26354 679.06109 pulse (J/cm.sup.2) Average power (W) 4 60 Dwell time (s) 100 2000

(82) Table 3 below gives a possible combination of parameters for an exemplary embodiment of a pulse according to the invention.

(83) TABLE-US-00003 TABLE 3 Repetition frequency (Hz) 40,000 Duration of the sub-pulse (s) 25 On time of the sub-pulse (s) 3 Off time of the sub-pulse (s) 22 Duty Cycle (%) of the sub-pulse 12% Peak power of the sub-pulse (W) 45 Energy of the sub-pulse (mJ) 0.75 Total energy of the train of pulses (mJ) 30.0 Number of pulses per train (i.e. per composite pulse) 40 Spot diameter (m) 150 Spot area (cm.sup.2) 0.0001767146 Fluence of the single sub-pulse (J/cm.sup.2) 4.24 Fluence of the composite pulse (J/cm.sup.2) 169.76527 Average power (W) 30 Dwell time (s) 1000

(84) FIG. 9 schematically shows the trend over time of the laser emission in an improved embodiment of the invention. Once again, the time is indicated on the abscissa and the power emitted is indicated on the ordinate. As can be seen in the diagram of FIG. 9, in this case each laser pulse is still a composite pulse, in the sense that emission is non-continuous in the emission time interval -on, but rather characterized by sub-pulses. Hereinafter, the composite pulse of FIG. 9 is named D pulse and is indicated with DP. FIG. 9 shows a sequence of pulses DP with the period T. A period T has an on-interval -on and an off-interval -off. The sum of the time intervals -on and -off is equal to the period T of the pulse DP. The relation -on/T is defined as the duty cycle of the composite pulse DP. The inverse 1/T of the period T of the composite pulse DP is defined as the frequency of the composite pulse DP.

(85) According to some embodiments the frequency of the composite pulse DP, hereinafter also defined as base frequency, is comprised between 1 and 1000 Hz, for example between 1 and 500 Hz. The duty cycle of the composite pulse DP can be comprised between 1% and 90% and preferably between 2% and 50% and even more preferably between 2% and 40%.

(86) As can be observed in FIG. 9, the interval -on of each composite pulse DP contains: a sub-pulse of greater duration and a train of sub-pulses of lesser duration, preferably equal for each of said shorter sub-pulses. Hereinafter the sub-pulse of greater duration will be indicated as pre-pulse (Pi) or hyper-energy pulse and the subsequent sub-pulses of lesser duration will be indicated as sub-pulses or hypo-energy pulses Si. The portion of the on interval -on of the composite pulse DP that follows the pre-pulse Pi is hereinafter also called tail. Therefore, each composite pulse DP is in turn constituted by a pre-pulse Pi, by a train of sub-pulses Si and by an off interval -off. According to one aspect, hyper-energy pulse is intended as a pulse with an energy per unit of surface area such as to generate plasma to remove the epidermis but such as not to interact with the middle layers of the dermis. Hypo-energy is intended as a pulse or sub-pulse with an energy per unit of surface area adapted to generate a cold ablation, i.e. without plasma or substantially without plasma, but of sufficient intensity to cause hyperemia and shrinkage of the collagen fibers of the deep levels of the dermis.

(87) In some embodiments, as shown schematically in FIG. 9, the pre-pulse or hyper-energy pulse Pi has a higher peak power than the hypo-energy pulses or sub-pulses Si. For example, the peak power of the latter is from 15 to 70% lower than the peak power of the former.

(88) It would also be possible for the pulses Si and Pi to have the same peak power.

(89) The sum of the time intervals -on and -off is equal to the period T of the pulse. The relation -on/T is defined duty cycle of the composite pulse. The inverse 1/T of the period T of the composite pulse is defined frequency of the composite pulse. According to some embodiments, the frequency of the composite pulse, hereinafter also defined as base frequency, is comprised between 1 and 1000 Hz, for example between 1 and 500 Hz. The duty cycle of the composite pulse can be comprised between 1% and 90% and preferably between 2% and 50% and even more preferably between 2% and 40%.

(90) In some embodiments the sub-pulses Si have a frequency comprised between 1 kHz and 200 kHz. In preferred embodiments, the frequency of the sub-pulses is comprised between 1 kHz and 100 kHz, and even more preferably between 2 kHz and 50 kHz. In some embodiments the frequency is comprised between 5 and 45 kHz, for example between 8 and 40 kHz.

(91) In some embodiments, the pre-pulse Pi has a duration comprised between 10 and 100 microseconds. In improved embodiments of the invention, the pre-pulse has a duration comprised between 20 and 90 microseconds and in particular between 40 and 80 microseconds. Currently, the preferred duration of the pre-pulse is comprised between 50 and 70 microseconds. Optimal results were obtained with a pre-pulse duration of around 60 microseconds.

(92) The duty cycle of the sub-pulses forming the tail of the pulse DP, i.e. the relation between the period of the sub-pulse, indicated with Ts in FIG. 9, and the duration of the on interval of the sub-pulse Si, is determined as a function of the peak power, of the duration -on of the composite pulse and of the energy per pulse that is required to be emitted at each pulse.

(93) The duty cycle of the sub-pulses can be comprised between 1% and 90%, preferably between 2 and 50%, more preferably between 2 and 40%.

(94) The peak power of the pre-pulse Pi, indicated as Peak Power in FIG. 9, can be comprised between 100 and 500 W, and preferably between 150 and 500 W. In some embodiments the peak power is comprised between 200 and 400 W, for example between 250 and 350 W. It would also be possible to adopt higher peak powers, for example comprised between 250 and 500 W.

(95) The peak power of the sub-pulses or hypo-energy pulses Si can be substantially lower, for example comprised between 20 and 250 W, preferably between 100 and 250 W.

(96) The energy of the pre-pulse can be comprised, for example, between 10 and 40 mJ and preferably between 12 and 25 mJ, even more preferably between 12 and 20 mJ.

(97) In some embodiments the total energy of the train of sub-pulses Si is comprised between 0.4 and 200 mJ, for example between 0.4 and 150 mJ and preferably between 0.4 and 130 mJ.

(98) The energy of the single sub-pulse Si can be comprised between 0.1 and 10 mJ and preferably between 01 and 8 mJ.

(99) The number of hypo-energy sub-pulses Si of each composite pulse is variable for example from 1 to 100 and preferably is greater than 1 and equal to or lower than 80. The spot area, i.e. the area of the section of the laser beam on the surface on which the beam is projected, is advantageously comprised between 0.0001 and 0.0003 cm.sup.2 and preferably between 0.00015 and 0.0002 cm.sub.2. According to some embodiments the diameter of the spot is comprised between 50 and 500 micrometers, preferably between 80 and 400 micrometers, even more preferably between 100 and 200 micrometers, for example around 150 micrometers. The fluence, i.e. the energy per unit of surface area, is obtained as the ratio between the powers and the spot areas indicated above and can be calculated for the pre-pulse or hyper-energy pulse Pi, for each sub-pulse or hypo-energy pulse Si and for the whole train of sub-pulses Si, on the basis of the spot area and of the energy emitted in the interval considered (Pi, single Si or sum of the pulses Si).

(100) The following tables 4 and 5 each indicate two series of values for the main parameters of the pulse. It must be understood that each parameter may vary in the interval defined by the two values of the corresponding line.

(101) TABLE-US-00004 TABLE 4 Repetition frequency of the 10,000 10,000 sub-pulse Si (Hz) Duration of the sub-pulse (s) 100 100 On time of the sub-pulse Si 4 24 (s) Off time of the sub-pulse Si 96 76 (s) Duty Cycle (%) 4% 24% Peak power pulse Si (W) 100 250 Energy of the single sub- 0.4 6.0 pulse Si (mJ) Sum of the energy of the train 0.4 120.0 of pulses Si (mJ) Number of the pulses Si in a 1 20 composite pulse Spot diameter (m) 150 150 Spot area (cm.sup.2) 0.001767146 0.0001767146 Fluence of the single sub- 2.26 33.95 pulse Si (J/cm.sup.2) Total fluence of the train 2.26 679.06 of pulses Si (J/cm.sup.2) Average power (W) 4 60 Average power pulse 154 67.5 Dwell time (s) 100 2000

(102) TABLE-US-00005 TABLE 5 Repetition frequency of the 40,000 40,000 sub-pulse Si (Hz) Duration of the sub-pulse (s) 25 25 On time of the sub-pulse Si 1 6 (s) Off time of the sub-pulse Si 24 19 (s) Duty Cycle (%) 4% 24% Peak power of the pulse Si 100 250 (W) Energy of the single sub- 0.1 1.5 pulse Si (mJ) Sum of the energy of the train 0.4 120.0 of pulses Si (mJ) Number of the pulses Si in a 4 80 composite pulse Spot diameter (m) 150 150 Spot area (cm.sup.2) 0.0001767146 0.0001767146 Fluence of the single sub- 0.57 8.49 pulse Si (J/cm.sup.2) Total fluence of the train 2.26 679.06 of pulses Si (J/cm.sup.2) Average power (W) 4 60 Average power pulse 154 67.5 Dwell time (s) 100 2000

(103) The following table 6 indicates an example of the values of the aforesaid parameters:

(104) TABLE-US-00006 TABLE 6 Repetition frequency of the sub-pulse Si (Hz) 40,000 Duration of the sub-pulse (s) 25 On time of the sub-pulse Si (s) 3 Off time of the sub-pulse Si (s) 22 Duty Cycle (%) 12% Peak power of the pulse Si (W) 23 Energy of the single sub-pulse Si (mJ) 0.375 Sum of the energy of the train of pulses Si (mJ) 15.0 Number of the pulses Si in a composite pulse 40 Spot diameter (m) 150 Spot area (cm.sup.2) 0.0001767146 Fluence of the single sub-pulse Si (J/cm.sup.2) 2.12 Total fluence of the train of pulses Si (J/cm.sup.2) 84.88 Average power (W) 15 Average power pulse 30 Dwell time (s) 1000

(105) Table 7 below indicates an example of the values of the significant parameters of the pre-pulse or high energy pulse Pi, usable in combination with the parameters of the pulses Si indicated above:

(106) TABLE-US-00007 TABLE 7 On time of the sub-pulse Si (s) 60 Peak power of the pulse Pi (W) 300 Energy of the single sub-pulse Pi (mJ) 15 Spot diameter (m) 150 Spot area (cm.sup.2) 0.0001767146 Fluence of the single sub-pulse Pi (J/cm.sup.2) 84.88 Average power (W) 250

(107) The period T of the composite pulse is given by the sum of the off-period -off and of the on-period -on, in turn given by the sum of the periods of the pulses Pi and Si. The off-period can be comprised between 0.1 and 5 ms, preferably between 0.5 and 2 ms, and even more preferably between 0.8 and 1.2 ms, for example around 1 ms.

(108) Given a portion of epidennis to be treated, the treatment is carried out by firing a train of pulses SP or DP in a plurality of points according to a given pattern on the surface to be treated. The dwell time of the laser in a given point of the pattern determines, together with the repetition frequency of the composite pulses (i.e. the inverse of the period T) the number of composite pulses applied in a given point of the pattern.

(109) The spacing of the points for applying the laser beam can be comprised between 50 micrometers to 1000 micrometers and preferably between 90 and 550 micrometers.

(110) For sufficiently high laser intensities and very short laser pulse durations, the laser-tissue interaction process is mediated by the fonnation of plasma in proximity of the irradiated surface. Plasma is defined as a macroscopically neutral gaseous phase with a large fraction of ionized particles.

(111) In the optical breakdown process, the photons of the laser pulse generate, in the vicinity of the irradiated surface, a certain number of electrons due to ionization of the molecules that have absorbed them; the intense electrical field of the laser pulse accelerates them greatly and, in a few nanoseconds, the avalanche ionization process that begins can enable very large electron densities, in the order of 10.sup.20 electrons/cm.sup.3 (dense plasma) and very high plasma temperatures, in the order of 10.sup.4 C., to be reached. In these conditions, the plasma is optically opaque, with subsequent shielding of the surface of the tissue from the incident beam, due to the high absorption coefficient of the ionized region. Subsequent expansion of the plasma generates a shock wave, which can cause fragmentation and local breakdown of the tissue.

(112) FIG. 17 (taken from Green H A, Domankevitz Y, Nlshoka N S. Pulsed carbon dioxide laser ablation of burned skin: in vitro and in vivo analysis. Laser Surg Med. 1990; 10(5):476-84) shows the percentage of plasma fonnation as a function of the fluence of the CO.sub.2 laser. As can be noted, for pulses with energy density comprised between 40-50 J/cm.sup.2 the percentage of plasma is very high and the cut is mediated by the plasma itself. Instead, for pulses with low fluence 1-10 J/cm.sup.2 the percentage of plasma is more or less negligible and the cut is mediated mainly by laser radiation. In the first case, it is the plasma, generated by the laser itself that produces its biological effects, while in the second case, the laser beam vaporizes the tissue directly. In the first case the temperatures involved are very high, in the order of 10,000 C. with very short dwell times (ns). In the second case, the temperatures involved are in the order of 1,500-2,000 C. but the times are longer (ms). The biological effects obtained in the two cases are very different from each other.

(113) Plasma vaporization is generally preferred to laser vaporization due to its high precision, the very clean residual tissue (as it induces minimum lateral thermal damage) and, above all, due to the almost total absence of charring. In fact, for example in comeal surgery, where the precisions involved must be extremely high, plasma ablation is currently the absolute gold standard. Moreover, when high peak intensities are used, besides being affected by non-negligible thermal effects, laser ablation also suffers fromphotomechariical effects that limit controllability of the cut by the operator. Instead, in the case of the present invention the photomechanical effects are a positive element as synergistic with the thermal effects for the desired shrinkage of the collagen fibers to be induced to obtain tissue shrinkage.

(114) The main object of some embodiments of the invention is to reach the deep layers of the dermis with the least possible heat front, to induce the least possible lateral thermal damage but, at the same time, which is able to stimulate hyperemia and shrinkage of the collagen fibers. It is known that both phenomena can be activated at medium-low temperatures, i.e. in the interval of 40-70 C. Pulses above the threshold of around 19 J/cm.sup.2 are capable of generating plasma and therefore generate, in the ablation cavity, temperatures of over 7,000 dc. Around the ablation cavity generated by the plasma (hemispherical shaped), the matter is so destructured that lateral thermal damage is minimum and the tissue is unable to contract. In fact, the collagen fibers are destroyed and the capillaries are dehydrated (for this reason there is no bleeding despite reaching the papillary dermis).

(115) The pulse structured according to the present invention makes use of a hyper-energy laser pulse capable of generating plasma to ablate the portion of epidermis with the least possible lateral thermal damage, thereby reducing to minimum correlated collateral effects, such as re-epithelialization defects due to the presence of carbonaceous residues or to excessive lateral thermal damage. However, on the other hand, excessive increase in heat around the ablation cavity causes widespread collagen destructuring, and in order to find collagen capable of contracting and functional capillaries it is necessary to move away from the ablation cavity by at least a hundred micrometers.

(116) Vice versa, pulses under 19 J/cm.sup.2 on average cause a minimum ablation cavity, ensure collagen contraction (shrinkage) even around the ablation cavity and however induce minimum vasodilation of the capillaries as the energy content emitted is decidedly low.

(117) To overcome this limit the stack technology was introduced in the past; this involves multiple repetitions of the aforesaid low energy pulses on each point. This made it possible to reach considerable depths, but to the detriment of tolerability, going against the minimally invasive logic of fractional technology.

(118) Starting from these considerations, with a pulse structured according to the present invention it is possible to eliminate the drawbacks of prior art and significantly increase the results on treated tissue. In particular, the D-type pulse defined above enables plasma mediated ablation to be combined with laser ablation.

(119) As plasma is photo-absorbent and reduces the ablation efficiency of the laser, the ideal fluences to obtain cold laser ablation vary in the interval of 4-19 J/cm.sup.2. Acting with fluences in this interval, 20-40 m of tissue per pulse is removed. In the D-type pulse, a series of hypo-energy sub-pulses Si (4-19 J/cm.sup.2) forming the tail of the composite pulse, is preceded by a single hyper-energy pulse (40 J/cm.sup.2) (pre-pulse Pi) capable of generating plasma to remove the epidermis but not such as to interact with the middle layers of the dermis. The hyper-energy pre-pulse Pi is then followed by a train of ablative hypo-energy laser pulses or laser sub-pulses Si, capable of generating cold ablation, but also of efficaciously inducing the hyperemia and shrinking effects of the collagen fibers located in the deep levels of the dermis.

(120) According to some embodiments the D-type composite pulse is designed by a hyper-energy body or pre-pulse Pi which, according to the curves elaborated by Green (FIG. 17), is capable of generating only plasma. This pre-pulse Pi is followed immediately by a tail of sub-pulses Si, i.e. small hypo-energy pulses. In some embodiments the hyper-energy pre-pulse is characterized by an energy of 15 mJ, by an on-time -on of 60 s, by a peak power of 250 W, by a spot (i.e. a circular area of incidence on the skin) with a diameter of 200 m and consequently by an energy per unit of surface area of 47.7 J/cm.sup.2. The subsequent sub-pulses Si can be characterized by an energy per pulse of 3 mJ, by an on-interval of 24 s, by a peak power again equal to 250 W, by a spot diameter of 200 m and consequently by an energy per unit of surface area of 9.5 J/cm.sup.2.

(121) The concept underlying the invention relates in general to implementation of a technology which is the result of combining different technologies with one another, in virtue of the knowledge of the various physical-biological phenomena taking place, according to precise relations of proportionality, both time- and space-related.

(122) In this regard, again within the scope of regenerating and rejuvenating cosmetic treatments or of treatment for disfiguring scarring, it would also be possible to combine medical products, such as gels containing growth factors or bio-stimulating pharmaceutical products, with fractional technology. The limit of conventional fractional technology consists in the chemical-physical characteristics of lateral thermal damage induced by laser ablation not mediated by plasma. In fact, in these conditions the residual tissue is subject to hyalinization phenomena and represents an obstacle to the diffusion of the aforesaid products applied to the epidermis after laser treatment.

(123) These limits are overcome by the use of an S-type pulse as defined above. As indicated above, the S-type pulse comprises a series of sub-pulses, for example characterized by a spot diameter of 150 m and by an energy per unit of surface area comprised between 1 and 35 J/cm.sup.2, for example comprised between 2 and 20 J/cm.sup.2, preferably between 2 and 15 J/cm.sup.2. These sub-pulses are therefore characterized by energies just above the threshold for significant plasma formation. In fact, plasma is photoabsorbent and therefore it would be counter-productive to emit energy per pulse greatly above this threshold. At these fluences the percentage of pulses forming plasma is significant and, according to Green (1990), is around 30%. A pulse thus obtained, as can be observed in the histologies, induces the formation of a hemispherical shaped crater. The main characteristic, which can be observed histologically, is that this crater is clean, with negligible thermal damage and optimal elasticity both of the margins and of the edges of the cavity. All this can contribute to make the cavity extremely receptive to the application of optional medicated products.

(124) Characteristics of the RF Current

(125) As described with reference to FIGS. 6 and 7, the optical radiation generated by the laser source can be combined with the application of radio frequency current through at least one electrode. The electrode is preferably integral with the same handpiece on which the laser emitter is located. Although a second electrode for closing the electrical circuit can also be provided, to be applied at a distance from the first, for example as an electrode to be placed in connection with a limb of the subject to which the treatment is applied, to obtain a concentration of the currents in the zone of the tissue to be treated, which is located in the zone of incidence of the laser beam, it is preferable to use two electrodes placed close to each other, preferably both carried by the same handpiece that also carries the laser emitter. In some embodiments, as shown in FIG. 6, the electrodes are adjacent to the zone irradiated by the laser source.

(126) In some embodiments the radio frequency current has a frequency comprised between 50 and 1000 kHz and preferably between 100 and 700 kHz. In currently preferred embodiments the frequency of the current is comprised between 400 and 600 kHz and even more preferably between 450 and 550 kHz. Application of the radio frequency current can normally have a duration that is longer than the laser radiation application time. Typically, the emitting time of the radio frequency current is comprised between 1 and 10 seconds. In preferred embodiments, the application time is comprised between 2 and 5 seconds. For reasons that will be apparent below, emission of the radiofrequency current does not start before application of the optical radiation by the laser source. Preferably, application of the laser radiation starts before application of the radio frequency current. In some embodiments, emission of laser radiation stops before application of the radio frequency current starts. In fact, the synergistic effect between the application of the two energy forms is presumably achieved as a result of the changes induced by the laser on the vascularized tissue, said changes facilitating the subsequent flow of radio frequency electrical current in the volume of the tissue in which the application of this energy is required.

(127) The power of the current emitted can advantageously be comprised between 5 and 100 W. In preferred embodiments the power is comprised between 10 and 50 W.

(128) Combination of two different energy forms (optical and RF current), appropriately combined with each other in time and space, enables deep transfer of the quantity of energy capable of exceeding the activation threshold of the biological processes typical of tissue repair. The energy applied in the form of radiofrequency current emitted separately would not be capable of activating any biological process. At the same time, unless extremely invasive parameters (stack 3-5) are used, laser radiation alone would not be capable of reaching the reticular dermis in a quantity sufficient to significantly activate these processes.

(129) Particularly advantageous embodiments of the invention provide for symbiotic energy combination so as to obtain a synergistic biological effect of the two energy forms, optical (laser) and electric (radio frequency current). In other words, this combined emission of different energies, optical and RF current, gives rise to greater biological effects than the simple sum of the single energies emitted. Therefore, the time relationship between the single elements involved is important.

(130) With reference to the rationale of the origin of the D-pulse, comprising a plasma ablation hyper-energy pre-pulse followed by a train of hypo-energy sub-pulses with laser-ablation effect, it can be observed that the RF current flows from the intact epidermis, due to the heat wave of the proximal portion of the ablation cavity generated by the plasma, to the ablation cavity generated by the laser pulse and from here jumps easily into the dilated capillaries that surround said cavity (see FIG. 12C).

(131) The current jump directly from the epidermis to the surface capillaries is more difficult as these are located at about a hundred micrometers from the healthy epidermis and from the ablation cavity generated by the plasma. The sequence of the phenomena, laser ablation and RF current application, is very important for optimizing the phenomenon.

(132) The application sequence, i.e. the time relations between the energies involved, plays an important role in the combination of the two energy forms. According to a possible interpretation of the action mechanism of the two energy forms applied, which is indicated here to provide an explanation of the synergistic effects obtained with the invention, but which must not be considered limiting, there is a close correlation between the two energies, dependent on concatenation of the biological events caused by them, which cannot be neglected to obtain a high treatment efficiency. The loss of efficiency could result in an unbalanced or excessive energy emission, which goes against the principles that inspire fractional technology with RF.

(133) According to a possible interpretation of the phenomena caused by combined emission of the two energy forms, which is provided here as possible explanation, but which the concepts underlying the invention are not bound to or dependent on, after laser ablation (mediated or not by plasma) a transitory ischemia followed by persistent hyperemia occurs, as represented schematically in FIG. 18.

(134) In the diagram of FIG. 18 the time is indicated in the abscissa. As shown in the figure, the laser pulse is followed by a fonnation of vapors and plasma and by an ischemia of the tissue in the time interval from 0.01 to 0.1 seconds after the rising front of the pulse (in the case of composite pulses such as those described here, this is intended as the rising front of the pre-pulse in the case of composite D-type pulse, or the rising front of the first sub-pulse in the case of composite S-type pulse).

(135) In the subsequent 24 hours there is an intense hyperemia of the tissue. Moreover, after the first half second, intense exudation takes place and plugs of exudate and keratin (crusts) form. The diagram shows the trends over time of the conductivity of the epidennis and of the dennis. As indicated in the diagram, it can be observed that the conductivity of the epidermis is generally greater than that of the dermis up to an instant (from a few tenths up to more than one second from the start of application of the laser pulse), in which the conductivity values are inverted, with the dermis that becomes more conductive than the epidermis. The instant in time in which the two curves cross over is the optimal moment for starting application of the energy in the form of RF current. Typically, the radio frequency current can be applied starting from 0.8-1.2 seconds after the rising front of the laser pulse.

(136) In fact, in the preceding instants there is an excessive gap in the conductivity between epidermis and dermis. To obtain a significant therapeutic effect, this impedance jump imposes the application of very high quantities of RF current, greater than those sufficient if the current is emitted starting from the cross-over point of the aforesaid electrical conductivity curves.

(137) In this regard, to induce homogeneous hyperemia of the capillaries of the papillary dermis, the distribution in space of the heat waves generated by the laser radiation assumes considerable importance. In fact, it is important that the spots are distributed with the greatest possible distance between them, although still capable of ensuring a certain degree of overlapping of the heat fronts of the dermis. This ensures that all the capillaries will be involved by the phenomenon of vasodilation and the current can thus flow adequately through them to the reticular dermis.

(138) Effects of the New Laser Pulses, Optionally in Combination with RF Current

(139) Numerous clinical studies have been carried out to evaluate the effects of the new shapes of laser pulses described above, separately or combined with the application of radio frequency current, in order to highlight their multiple ameliorative aspects over the prior art.

(140) Typical applications relate to aesthetic treatments of the skin, in particular with the object of obtaining a reduction of wrinkles, finning and overall rejuvenation of the tissue.

(141) In order to evaluate the different effects on tissue of the laser pulses SP and DP described above, in vivo tests were carried out on a sheep.

(142) FIGS. 10A-10K show a selection of the results attained. Each figure indicates the type of pulse used (DP or SP), the duty cycle of the composite pulse, indicated as burst and expressed in percentage, the energy emitted per pulse expressed in mJ and the duration in microseconds of the composite pulse applied.

(143) The microphotographs illustrated in FIGS. 10A-10K, in particular, show the effect of tissue ablation at the axis of the optical laser beam applied and the shrinkage effect. As can be observed from the histologies in all the photographs indicated in FIGS. 10A-10K, the SP pulse and the DP pulse differ considerably as far as the shape of the ablation zone, and shrinkage effect in the tissue surrounding the central zone involved by laser beam, are concerned. The subsequent FIGS. 11A and 11B show a schematic representation of the ablation and shrinkage effect obtained respectively with the SP pulse (FIG. 11A) and with the DP pulse (FIG. 11B). FIGS. 12A and 12B schematically show the heat bubble that is generated in the tissue in the two cases (FIG. 12A for the SP pulse; FIG. 12B for the DP pulse).

(144) As can be observed from FIGS. 10-12, the SP pulse generates an ablation zone in the papillary dermis (PD) with modest shrinkage effect, while the DP pulse generates an ablation zone, again limited to the layer of papillary dermis (PD), but much deeper. The ablation zone is surrounded by a surrounding area in which the papillary dermis has undergone substantial contraction or shrinkage. From the viewpoint of heat (FIGS. 12A and 12B), it can be observed that the SP pulse generates a heat bubble, i.e. a thermal heating front of the tissue, which involves the thickness of the papillary dermis and laps the reticular dermis RD below. The DP pulse, characterized by the pre-pulse and by the tail formed by a sequence of high frequency sub-pulses, generates a heat bubble, i.e. a heat front represented in FIG. 12B, which besides passing through the papillary dermis also deeply penetrates the reticular dermis below.

(145) FIG. 12C schematically shows the effect of ablation of the optical radiation in the two modes: direct laser ablation and plasma mediated ablation. The first cavity excavated in the epidermis is generated by plasma mediated ablation (indicated as plasma ablation cavity in the figure). The deepest part of the ablation (indicated as laser ablation cavity in the figure) is obtained by direct ablation using the laser beam. The figure also shows the zones involved by the heat wave generated by plasma and by the heat wave generated by laser radiation. As can be observed, the zone hit by the heat generated during the laser ablation phase (not plasma mediated) is located at a greater depth from the epidermis and penetrates the tissue in which there is greater density of blood vessels, which as a result of the radiation undergo dilation and hyperemia.

(146) The effect of this increased penetration is an intense stimulation of the blood supply and consequently intense hyperemia of the tissue. Thermal stimulation of the reticular dermis also causes increased shrinkage of the surface layers of the papillary dermis.

(147) The results illustrated above refer to applications of laser energy alone. The combination of laser radiation (emitted in the form of composite DP-type or SP-type pulses) with the emission of radio frequency electrical current makes it possible to obtain an improvement of the treatment effects.

(148) Penetration of the radio frequency current in the tissue depends on the frequency of the current applied, on the magnetic permeability of the tissue and on the conductivity of the tissue according to the formula:

(149) $=\frac{1}{\sqrt{f}}$
where:
is the standard penetration depth expressed in m
=3.14
f is the frequency in Hz
is the magnetic penneability expressed in Henry per meter
is the electrical conductivity expressed in Siemens per meter.

(150) FIG. 13 shows the trend of the electrical conductivity (expressed in S/m) as a function of the frequency of the current for the following tissues or structures:

(151) BV: blood vessels

(152) WS: wet skin

(153) F: adipose tissue

(154) DS: dry skin

(155) It can be observed in the diagram of FIG. 13 that the maximum conductivity is that of the blood vessels.

(156) In the absence of ablation treatment and of vasodilation, the radiofrequency current flows for about 90% through the epidermis and only for 10% along the blood vessels. Following stimulation of the tissue by laser irradiation and above all as a result of ablation resulting from irradiation of the epidermis with the laser pulses, a substantial improvement of the radio frequency current flow conditions is obtained.

(157) Vasodilatation is mainly due to two effects: a first immediate effect is heating due to the heat wave. Heating of the blood vessels causes immediate vasodilation as a result of thermal effect. A second slower and more persistent effect is due to the action of the laser on neuro-modulating factors. This effect occurs with a delay compared to the first and has greater persistence over time.

(158) Regardless of which of the two effects are used, vasodilation contributes to an increased flow of current through the blood vessels and consequent reduction in the flow of current in the surface layers (epidermis) of the skin. This is due both to the decrease in the distance between vessel walls and outer surface of the epidermis, and to the increased cross section of the vessel. Moreover, the formation of ablation cavities reduces locally, i.e. at the micro-hole obtained by the laser ablation effect on the tissue, the distance between outer surface of the epidermis and blood vessels. This enables more efficient deep penetration of the radio frequency current. The formation of plasma in the ablation cavity, resulting from the localized increase in temperature caused by the laser, further improves electrical transmission.

(159) Typically, from a distribution of 90% of current on the surface and 10% in the blood vessels, a distribution of around 60% of the radio frequency current flowing at the level of the epidermis and 40% at the level of the blood vessels can be obtained as a result of the application of laser energy.

(160) This increased flow of electrical current in the deep tissues causes deep hyperemia. This deep hyperemia in turn supplies the hyperemia of more superficial tissue, even after emission of energy from the outside has ceased.

(161) The quantity of hemoglobin provides an indication of the level of tissue hyperemia. FIGS. 14A to 14E show diagrams of the trend of the variation in percentage of hemoglobin over time following treatment with laser or with laser and radio frequency current according to the invention. The diagrams highlight the different effect of the various types of treatment with one or other of the two SP and DP pulses described above, with or without the application of radiofrequency current. The trend over time of the percentage of hemoglobin is indicative of the trend over time of the hyperemia. With an increase in blood flow, and consequently of hyperemia, there is an increase in hemoglobin. The abscissa indicates the time (not in scale) from the treatment and the ordinate indicates the variation in the percentage of hemoglobin starting from a base value corresponding to the origin of the ordinate (hemoglobin content before the treatment).

(162) The parameters used to obtain the results indicated in these figures are the following:

(163) average pulse power: 30 W

(164) peak power: 250 W

(165) D-pulse with pre-pulse Pi of 60 microseconds followed by 40 sub-pulses Si;

(166) S-pulse with 40 sub-pulses

(167) Stack 1 (one composite pulse)

(168) Dwell time 1 ms

(169) Energy per pulse 0.75 mI

(170) Radiofrequency energy: 30 W for 3 seconds at 500 kHz.

(171) More in particular, FIG. 14A indicates two curves, marked with SP and DP, which show the trend of the variation of hemoglobin percentage over time following treatment with laser alone with SP-type pulses and with DP-type pulses respectively, without the application of radio frequency current. It can be observed that in both cases the quantity of hemoglobin increases following the treatment and has a peak at around 18-20 hours after the treatment. However, in the case of treatment with DP-type pulse, the peak is much lower. In practice, this corresponds to a lower impact of the cosmetic treatment on the patient and consequently fewer negative side effects, such as reddening and swelling.

(172) After passing the hyperemia peak within 24 hours from application, the hemoglobin values drop to levels that exceed the base values (pre-application) by less than 40%. However, it is noted that in the long term, more than 72 hours after the treatment, the hyperemia caused by treatment with DP-type pulses tends to remain above the base value, increasing slightly, while hyperemia caused by conventional pulses tends to decrease, returning toward the pre-application value.

(173) In practice, this means that the treatment with DP pulses is less invasive, causing fewer undesirable side effects in the short time, but maintains the level of hyperemia at values above normal for longer times. This enables a longer lasting effect of stimulation of the biological processes that lead to the desired results of rejuvenation and toning of the tissue.

(174) FIG. 14B compares the effects on the percentage content of hemoglobin obtained by the application of laser energy with SP pulse (SP curve) with those obtained by the combined application of laser pulses of SP-type and radiofrequency current.

(175) It can be observed that by applying radio frequency in combination with the SP-type pulse, there is a further reduction of the peak of increase of hyperemia. Therefore, an advantage of reduction of side effects is obtained in the short term (around 24 hours from application).

(176) In the long term (over 72 hours) an increased hemoglobin content is observed, which indicates an increased degree of hyperemia over time, in the case of combined laser+RF treatment. This corresponds to the fact that the energy emitted through radio frequency caused a deeper hyperemia, as the vasodilation caused by pre-treatment with the laser promoted the flow of electrical current in the deeper layers of the tissue, to the detriment of the flow in the outer layers of the epidermis. The deep hyperemia thus induced maintains a longer lasting effect over time, although reducing the hyperemia peak in the short term.

(177) FIG. 14C compares the effect, in terms of variation of the percentage of hemoglobin content, of the laser beam with DP pulses alone (DP curve) and of the laser beam with DP pulses in combination with radiofrequency (DP+RF curve). It can be observed that the hyperemia peak in the 24 hours remains substantially unvaried, i.e. it is not influenced either positively or negatively by the combined application of laser energy and radiofrequency electrical energy. In the long term, a minimum is reached followed by an increase in both cases, with a steeper trend in the case of combined application.

(178) The curves DP+RF and SP+RF of FIG. 14D show the trend of the variations of hemoglobin percentage over time in the case of combined application of laser+RF current in the two cases of SP pulse (SP+RF curve) and of DP pulse (DP+RF curve).

(179) Finally, FIG. 14E shows overlapping of the four curves SP, DP, SP+RF, DP+RF. An ideal curve Id, indicated with a dashed line, is overlapped on these four curves; this represents the ideal trend that the hyperemia should have to obtain minimum undesirable side effects and maximum treatment efficacy. It can be observed that the use of a DP pulse, or the combined use of one of the two DP or SP pulses with the application of radio frequency current provides hyperemia curves which are closer to the ideal curve and therefore more favorable. In particular, it can be observed that the shape of the DP pulse enables, even without the application of radio frequency current, a particularly efficient result to be obtained in terms of trend over time of the hyperemia.

(180) A hyperemia that lasts over time enables more efficient tissue repair to be obtained as a result of the effect of the hyperemia em pH values, temperature, NO, ptO.sub.2, ptCO.sub.2, O.sub.2, activation of cellular redox complexes, acute phase proteins, cytokines, cellular proliferation speed, cellular differentiation and cellular renewal speed.

(181) Besides the effects in terms of inducing hyperemia, and the trend over time thereof, another important factor in evaluating the efficacy of these treatments is the shrinkage effect on tissue and in particular on collagen. Shrinkage is an effect of considerable importance in treatments to rejuvenate the epidermis, reduce wrinkles, and to tone and firm tissue.

(182) Tests performed using the various combinations of pulses SP, DP and SP+RF, DP+RF gave results that are variable as a function of the type of treatment carried out. The degree of shrinkage can be determined simply by measuring the distance between points of the treatment pattern at the time of application and in a time interval subsequent to application. FIG. 15 indicates in the ordinate the average distance between the points of the pattern, i.e. the average distance between the centers of the marks of the laser beam for the four possible combinations indicated in the abscissa:

(183) SP: laser alone with SP pulse

(184) DP: laser alone with DP pulse

(185) SP+RF: laser with SP pulse in combination with radio frequency current;

(186) DP+RF: laser with DP pulse in combination with radio frequency current.

(187) The diagram indicates squares labeled Im and 120. The former indicate the values immediately after treatment, i.e. indicative of the shrinkage obtained as an immediate effect of the treatment on the tissue. The squares indicated with 120 indicate data collected 120 hours after treatment. The statistical significance of the data is marked with (***) (=significance greater than 99%) and ns (statistically insignificant data).

(188) In the diagram of FIG. 15 it can be observed that in the long term the effect in terms of shrinkage is greater in the case of combined laser+radiofrequency treatment, regardless of the type of laser pulse (SP, DP) used.

(189) The excellence of the treatment is also determined as a function of the time required for complete recovery of the subject treated, i.e. the time necessary for the traces of the treatment to disappear from the epidermis. The experimental results relating to this aspect are summed up in the diagram of FIG. 16.

(190) In this diagram the abscissa indicates the time expressed in days since treatment (origin of the abscissa). The ordinate indicates the percentage of plugs of exudate and keratin, hereinafter improperly called crusts, which persist over time Immediately after the treatment 100% of the crusts are visible. The four curves indicated with DP+RF, SP+RF, DP and SP show the trend over time of the reduction in the number of crusts. It can be observed in the graph that the treatment with laser alone and SP pulse causes greater persistence of these crusts, while combined treatment of laser radiation with DP-type pulse and radiofrequency current is characterized by a substantial decrease in the time required for the disappearance of a high percentage (80%) of the crusts. In the case of treatment with DP pulses and radio frequency, over 80% of the crusts had already disappeared 8-9 days after treatment, while in the case of application of laser alone with SP pulses the same level of decrease is only reached 13 days after treatment.

(191) While the embodiments described of the object illustrated here have been shown in the drawings and described in full in the above with particulars and details in relation to the different examples of embodiment, those skilled in the alt will understand that a number of modifications, changes and omissions are possible without departing from the innovative teachings, from the principles and from the concepts set forth above, and from the advantages of the object defined in the appended claims. Therefore, the effective scope of the innovations described-must be determined only on the basis of the widest interpretation of the appended claims, so as to comprise all modifications, changes and omissions. In addition, the order or sequence or any step of the method or process can be varied or rearranged according to alternative embodiments. In particular, it is possible to obtain the above-described synergistic effects from combination of the laser radiation and of the radio frequency current also using other shapes of laser pulse, such as a sequence of simple pulses, with appropriate repetition frequency.

(192) While specific embodiments of the invention have been shown and described in detail to illustrate the application of the principles of the invention, it will be understood that the invention may be embodied otherwise without departing from such principles.