MATERIAL PRETREATEMENT METHOD USING LOW-TEMPERATURE EXTRUSION AND MULTIENZYME SYNERGISTIC DEGRADATION

Abstract

A material pretreatment method using low-temperature extrusion and multienzyme synergistic degradation. A to-be-extruded material added with enzyme preparations and having a certain moisture content is blended before extrusion, the to-be-extruded material is fed into a screw extrusion device and is to be subjected to low-temperature extrusion treatment, multiple enzyme preparations are added in the material before extrusion, and starch, cellulose, protein and other substances in the material are degraded under the synergistic effect of the added multiple enzyme preparations in an extrusion process. Compared with the prior art, the yield of glucose and maltose converted from starch is improved, the residual oil rate of oil material meals is reduced, soluble substances of plant fiber materials are increased, and compared with the process of the traditional technology, the time needed in converting a protein material into a polypeptide material is shortened.

Claims

1. A material pretreatment method by low-temperature extrusion-multi-enzyme synergistic degradation, including preparing the material to be extruded added with enzyme preparations and having a certain moisture content before extrusion, wherein two or more enzyme preparations are added to the material before extrusion, and then, the material to be extruded is fed into a screw extrusion device for low-temperature extrusion processing to form an extrudate.

2. (canceled)

3. A material pretreatment method by low-temperature extrusion-multi-enzyme synergistic degradation, including preparing the material to be extruded added with enzyme preparations and having a certain moisture content before extrusion, wherein the added enzyme preparations include: two or more of added high-temperature-resistant ?-amylase, highly-efficient thermo stable ?-amylase, mesophilic ?-amylase, amyloglucosidase, glucoamylase, highly-efficient amyloglucosidase, potent amyloglucosidase, protease, complex enzyme, and two or more of added alkaline protease, neutral protease, acid protease, complex protease and papain; characterized in that the added enzyme preparations further include: two or more of complex amyloglucosidase, pullulanase, maltobiohydrolase, maltotriohydrolase, cellulase, hemicellulase, pectinase, xylanase, ?-amylase, fungal ?-amylase, fuel alcohol protease, alcohol-specific complex amyloglucosidase, and then, the material to be extruded is fed into a screw extrusion device for low-temperature extrusion processing to form an extrudate.

4. The material pretreatment method by low-temperature extrusion-multi-enzyme synergistic degradation according to claim 1, including preparing the material to be extruded added with enzyme preparations and having a certain moisture content before extrusion, the material to be extruded including: one or more of rice, degermed corn, corn with germ, sorghum rice, barley, wheat and their starch, and crude starch added with enzyme preparations, and one or more of cassava whole flour, potato whole flour and their starch, and crude starch added with enzyme preparations, characterized in that the material to be extruded further including: one or more starchy materials of sweet potato whole flour, millet, black kerneled rice, buckwheat, oat, rye and their starch, and crude starch added with enzyme preparations.

5. The material pretreatment method by low-temperature extrusion-multi-enzyme synergistic degradation according to claim 1, including preparing the material to be extruded added with enzyme preparations and having a certain moisture content before extrusion, characterized in that the material to be extruded including: one or more oily materials of soybean, corn germ, peanut, sunflower seed, rapeseed, sesame seed, yellow mustard seed, cottonseed, flax seed, castor seed, and perilla seed added with enzyme preparations.

6. The material pretreatment method by low-temperature extrusion-multi-enzyme synergistic degradation according to claim 1, including preparing the material to be extruded added with enzyme preparations and having a certain moisture content before extrusion, characterized in that the material to be extruded including: stem, leaf, pod, root, fruit vegetable fiber materials from one or more of corn, wheat, barley, rice, sorghum, rape, soybean plants added with enzyme preparations.

7. The material pretreatment method by low-temperature extrusion-multi-enzyme synergistic degradation according to claim 1, including preparing the material to be extruded added with enzyme preparations and having a certain moisture content before extrusion, characterized in that the material to be extruded including: one or more materials in the filtered mash residues from brewing beer with rice, degermed corn, sorghum rice, barley and wheat as auxiliary materials, one or more materials in the filtered residues of sugar liquid from producing syrup with rice, degermed corn, sorghum rice, barley and wheat as raw materials, one or more materials in the dregs or filtered residues from producing alcohol with rice, degermed corn, corn with germ, sorghum rice, barley and wheat as raw materials, one or more materials in the defatted cakes from producing oil with soy, corn germ, peanut, sunflower seed, rapeseed, sesame, yellow mustard, rice bran and cottonseed as raw materials, and one or more proteinaceous materials in soy protein powder, corn protein powder, barley protein powder, wheat protein powder, peanut protein powder, sunflower seed protein powder, rapeseed protein powder, rice bran protein powder, sesame protein powder and cottonseed protein powder.

8. (canceled)

9. The material pretreatment method by low-temperature extrusion-multi-enzyme synergistic degradation according to claim 1, including preparing the material to be extruded added with two or more enzyme preparations and having a certain moisture content before extrusion, and then, feeding the material to be extruded into a screw extrusion device for low-temperature extrusion processing, characterized in that the screw extrusion device comprises one or more of a single-screw extruder, a twin-screw extruder, and a tri-screw extruder.

10. (canceled)

11. The material pretreatment method by low-temperature extrusion-multi-enzyme synergistic degradation according to claim 2, including preparing the material to be extruded added with enzyme preparations and having a certain moisture content before extrusion, the material to be extruded including: one or more of rice, degermed corn, corn with germ, sorghum rice, barley, wheat and their starch, and crude starch added with enzyme preparations, and one or more of cassava whole flour, potato whole flour and their starch, and crude starch added with enzyme preparations, characterized in that the material to be extruded further including: one or more starchy materials of sweet potato whole flour, millet, black kerneled rice, buckwheat, oat, rye and their starch, and crude starch added with enzyme preparations.

12. The material pretreatment method by low-temperature extrusion-multi-enzyme synergistic degradation according to claim 2, including preparing the material to be extruded added with enzyme preparations and having a certain moisture content before extrusion, characterized in that the material to be extruded including: one or more oily materials of soybean, corn germ, peanut, sunflower seed, rapeseed, sesame seed, yellow mustard seed, cottonseed, flax seed, castor seed, and perilla seed added with enzyme preparations.

13. The material pretreatment method by low-temperature extrusion-multi-enzyme synergistic degradation according to claim 2, including preparing the material to be extruded added with enzyme preparations and having a certain moisture content before extrusion, characterized in that the material to be extruded including: stem, leaf, pod, root, fruit vegetable fiber materials from one or more of corn, wheat, barley, rice, sorghum, rape, soybean plants added with enzyme preparations.

14. The material pretreatment method by low-temperature extrusion-multi-enzyme synergistic degradation according to claim 2, including preparing the material to be extruded added with enzyme preparations and having a certain moisture content before extrusion, characterized in that the material to be extruded including: one or more materials in the filtered mash residues from brewing beer with rice, degermed corn, sorghum rice, barley and wheat as auxiliary materials, one or more materials in the filtered residues of sugar liquid from producing syrup with rice, degermed corn, sorghum rice, barley and wheat as raw materials, one or more materials in the dregs or filtered residues from producing alcohol with rice, degermed corn, corn with germ, sorghum rice, barley and wheat as raw materials, one or more materials in the defatted cakes from producing oil with soy, corn germ, peanut, sunflower seed, rapeseed, sesame, yellow mustard, rice bran and cottonseed as raw materials, and one or more proteinaceous materials in soy protein powder, corn protein powder, barley protein powder, wheat protein powder, peanut protein powder, sunflower seed protein powder, rapeseed protein powder, rice bran protein powder, sesame protein powder and cottonseed protein powder.

15. The material pretreatment method by low-temperature extrusion-multi-enzyme synergistic degradation according to claim 2, including preparing the material to be extruded added with two or more enzyme preparations and having a certain moisture content before extrusion, and then, feeding the material to be extruded into a screw extrusion device for low-temperature extrusion processing, characterized in that the screw extrusion device comprises one or more of a single-screw extruder, a twin-screw extruder, and a tri-screw extruder.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0082] The present invention provides a material pretreatment method by low-temperature extrusion-multi-enzyme synergistic degradation, including preparing the material to be extruded added with enzyme preparations and having a certain moisture content before extrusion, wherein the extrusion barrel temperature is ?80? C.; the moisture content of the material is ?70%; a number of enzyme preparations are added into the material before extrusion, including: two or more of ?-amylase, glucoamylase, maltase, pullulanase and protease. During the extrusion process of the material, the substances like starch, cellulose and protein in the material are degraded by the synergistic action of a variety of added enzyme preparations all the time.

[0083] Compared with the prior art, the present invention improves the yield of converting starch into glucose and maltose, reduces the meal residual oil rate after oil production by solvent extraction of oil materials, increases the soluble substances of vegetable fiber materials, and increases the protein extraction rate of proteinaceous materials. The above deficiencies of the prior art are solved.

[0084] The processing measures of the technology of the present invention are as follows:

[0085] Step 1: a material pretreatment method by low-temperature extrusion-multi-enzyme synergistic degradation, including preparing the material to be extruded added with enzyme preparations and having a certain moisture content before extrusion, wherein the added enzyme preparations include: one or more of added high-temperature-resistant ?-amylase, highly-efficient thermostable ?-amylase, mesophilic ?-amylase, amyloglucosidase, glucoamylase, highly-efficient amyloglucosidase, potent amyloglucosidase, protease, complex enzyme, etc., and one or more of added alkaline protease, neutral protease, acid protease, complex protease and papain; the added enzyme preparations further include: one or more of added complex amyloglucosidase, pullulanase, maltobiohydrolase, maltotriohydrolase, cellulase, hemicellulase, pectinase, xylanase, ?-amylase, fungal ?-amylase, fuel alcohol protease, alcohol-specific complex amyloglucosidase, and then, the material to be extruded is fed into a screw extrusion device for low-temperature extrusion processing; the screw extrusion device comprises one or more of a single-screw extruder, a twin-screw extruder, and a tri-screw extruder; the screw extrusion device has the extrusion barrel temperature of ?80? C.; the moisture content of the material to be extruded is ?70% before extrusion;

[0086] Step 2: preparing the material to be extruded added with enzyme preparations and having a certain moisture content before extrusion, the material to be extruded including: one or more of rice, degermed corn, corn with germ, sorghum rice, barley, wheat and their starch, and crude starch added with enzyme preparations, and one or more of sweet potato whole flour, cassava whole flour, potato whole flour and their starch, and crude starch added with enzyme preparations, and the material to be extruded further including: one or more starchy materials of millet, black kerneled rice, buckwheat, oat, rye added with enzyme preparations;

[0087] preparing the material to be extruded added with enzyme preparations and having a certain moisture content before extrusion, the material to be extruded including: one or more oily materials of soybean, corn germ, peanut, sunflower seed, rapeseed, sesame seed, yellow mustard seed, cottonseed, flax seed, castor seed, and perilla seed added with enzyme preparations;

[0088] preparing the material to be extruded added with enzyme preparations and having a certain moisture content before extrusion, the material to be extruded including: stem, leaf, pod, root, fruit vegetable fiber materials from one or more of corn, wheat, barley, rice, sorghum, rape, soybean plants added with enzyme preparations;

[0089] preparing the material to be extruded added with enzyme preparations and having a certain moisture content before extrusion, the material to be extruded including: one or more materials in the filtered residues of beer mash with rice, degermed corn, sorghum rice, barley and wheat as auxiliary materials added with enzyme preparations, one or more materials in the filtered residues of sugar liquid with rice, degermed corn, sorghum rice, barley and wheat as raw materials for syrup, one or more materials in the dregs or filtered residues with rice, degermed corn, corn with germ, sorghum rice, barley and wheat as raw materials for alcohol, one or more materials in the defatted cakes with soy, corn germ, peanut, sunflower seed, rapeseed, sesame, yellow mustard, rice bran and cottonseed as raw materials for oil production, and one or more proteinaceous materials in soy protein powder, corn protein powder, barley protein powder, wheat protein powder, peanut protein powder, sunflower seed protein powder, rapeseed protein powder, rice bran protein powder, sesame protein powder and cottonseed protein powder.

[0090] In one embodiment, the present invention relates to low-temperature extrusion multi-enzyme synergistic degradation of starch in a degermed corn extrudate, and can overcome the limitation of the aforementioned prior art about adding only one enzyme preparation during extrusion. For example, as to starchy materials, adding high-temperature-resistant ?-amylase can only degrade ?-1,4 glycosidic bond of starch, but cannot degrade ?-1,6 glycosidic bond of starch in degermed corn.

[0091] The technology of the present invention, low-temperature extrusionmulti-enzyme synergistic degradation of materials (such as starchy dry-process degermed corn), i.e., low-temperature extrusion of materials (such as starchy degermed corn) added with a number of enzyme preparations (such as high-temperature-resistant ?-amylase, glucoamylase and pullulanase), can overcome the above-mentioned deficiencies of the prior art, and increase the glucose content and the maltose content in syrup.

[0092] In another embodiment, the present invention provides a method for the preparation of proteins and polypeptides by low-temperature extrusion-multi-enzyme synergistic degradation of proteinaceous materials. Low-temperature extrusion multi-enzyme synergistic degradation of proteinaceous materials is just low-temperature extrusion of proteinaceous materials added with a number of enzyme preparations (such as alkaline protease, complex protease, cellulase) to convert proteins in the materials into polypeptides. Included are a method for preparing protein and polypeptide by low-temperature extrusion multi-enzyme synergistic degradation of protein, and a method of preparing a powdery protein or polypeptide product through carrying out conventional extraction.fwdarw.separation (single enzyme and multi-enzyme synergistic hydrolysis or alkali-solution and acid-isolation).fwdarw.purification.fwdarw.ultrafiltration.fwdarw.concentration.fwdarw.spray drying or freeze drying on pulverized substances obtained after low-temperature drying of extrudates of raw proteinaceous materials treated by low-temperature extrusion multi-enzyme synergistic degradation for preparing protein and polypeptide. The present invention is used for solving the problems of long process time, low protein extraction rate, low conversion rate of protein into polypeptide, high production cost, etc. for preparing proteins and polypeptides in the prior art.

[0093] It can be seen that for proteinaceous materials, when enzyme preparations such as alkaline protease, acid protease, neutral protease, complex protease, etc. are added, their optimum pH values are respectively different, and the types and characteristics of the enzymatically hydrolyzed proteins are different. During the extrusion process, the simultaneous addition of a number of enzyme preparations can overcome the limitations of enzymolysis of proteins by the addition of only one enzyme preparation. The process time required for the preparation of protein and polypeptide is less than the time required for the conventional process; the protein extraction rate is ?80%; the conversion rate of protein into polypeptide is greater than 80%; and the peptide fragments having the molecular weight of less than 1000 Da of the polypeptide product is ?75%.

[0094] In a further embodiment, the present invention relates to the addition of a number of enzyme preparations for the preparation of a raw material for protein and polypeptide before extrusion processing when preparing corn polypeptide powder and soy isolate protein powder, the enzyme preparation including: cellulase, hemicellulase, alkaline protease, neutral protease, acid protease, complex protease, etc. This can overcome the deficiencies brought by the addition of only one enzyme preparation, such as neutral protease or alkaline protease or acid protease, saying, the degradation of protein and the degree of protein's conversion into polypeptide are limited. The present invention can shorten the time required for the process of preparing proteins and polypeptides, increase the rate of protein extraction and the conversion rate of protein into polypeptide, shorten the time required to convert a protein material into a polypeptide material to ?-? of the time required for the conventional process, and enable the peptide fragments having a molecular weight of less than 1000 Da of the polypeptide product ?75%.

[0095] In a further embodiment, the present invention relates to the low-temperature extrusion multi-enzyme synergistic degradation processing of vegetable fiber materials of stem, leaf, pod, root, fruit and the like from one or more of corn, wheat, barley, rice, sorghum, rape, soybean plants, which can increase the reduction of cellulose, hemicellulose and lignin in vegetable fiber materials by 3%-50%, making the extrudate more favorable for application in the fields like feed processing, papermaking, ethanol production by fermentation, production of xylan and xylitol, production of xylooligosaccharide, and production of foods and medicines for diabetics.

[0096] In a further embodiment, the present invention relates to low-temperature extrusion-multi-enzyme synergistic degradation processing of oily materials like one or more oily materials of soybean, corn germ, peanut, sunflower seed, rapeseed, sesame seed, yellow mustard seed, cottonseed, flax seed, castor seed, and perilla seed before solvent extraction, which can accelerate the destruction of the cell walls of oily materials, so that the oil in the cells rapidly accumulates into oil droplets, and this can accelerate the entry of the solvent into the cells and the extraction of the oil from the cells, thus reducing the residual oil rate to 0.3%-1.0%.

[0097] The present invention has the following advantages over the prior art (the extrusion-enzymolysis technique with the addition of only one enzyme preparation and the low-temperature extrusion technique):

[0098] increasing the yield of converting starch in starchy materials into glucose and maltose by 0.5%-3%;

[0099] reducing the meal residual oil rate after oil production by solvent extraction of oil materials to 0.3%-1.0%;

[0100] increasing the soluble substances of vegetable fiber materials by 3%-30%;

[0101] shortening the time required to convert a protein material into a polypeptide material to ?-? of the time required for the conventional process, and enabling the peptide fragments having a molecular weight of less than 1000 Da of the polypeptide product 75%.

EXAMPLES

[0102] The following examples are intended to illustrate the present invention in more details, but are not to be construed as limitations to the scope of the present invention.

Example 1

[0103] A process for producing glucose syrup using the extrudate from low-temperature extrusion-multi-enzyme synergistic degradation of dry-process degermed corn.

[0104] The method for producing glucose syrup by the technology of the present patent is as follows:

[0105] First, the raw corn was degermed by a dry process to obtain dry-process degermed corn flour, which was adjusted to have a moisture content ?70%. At the same time, high-temperature-resistant ?-amylase, glucoamylase and pullulanase were added; the extrusion barrel temperature was adjusted ?80? C., and then, the material to be extruded was fed into a screw extrusion device for low-temperature extrusion processing. The extrudate was mixed with process water for producing syrup. The pH was adjusted, and high-temperature-resistant ?-amylase or mesophilic ?-amylase was added. The temperature was raised to 85? C.-95? C. and maintained for 1 min-20 min, and was then decreased to 55? C.-65? C. The above liquefied liquid was poured into a saccharification tank, followed by the pH adjustment and the addition of amyloglucosidase, for saccharification for 15 h. The resultant syrup had a DE value of about 100% and a glucose content DX of about 98%.

Example 2

[0106] A process for producing oil by the extrudate from the low-temperature extrusion-multi-enzyme synergistic degradation of cell walls in soybean by solvent extraction.

[0107] First, soybean was pulverized to obtain soybean flour, which was adjusted to have a moisture content ?70%. At the same time, cellulase, hemicellulase and pectinase were added; the extrusion barrel temperature was adjusted ?80? C., and then, the material to be extruded was fed into a screw extrusion device for low-temperature extrusion processing. The extrudate was dried until the moisture content was ?10%, and the dried soybean extrudate was introduced into a extractor to produce oil by solvent extraction, giving soybean meal and soybean oil. The residual oil rate of soybean meal was 0.3%-1.0%.

Example 3

[0108] A process for low-temperature extrusion-multi-enzyme synergistic degradation of vegetable fibers in stem, leaf, root and fruit of corn.

[0109] First, stem, leaf, root and fruit of corn were pulverized to obtain a pulverized material which was adjusted to have a moisture content ?70%. At the same time, cellulase, hemicellulase, pectinase and xylanase were added; the extrusion barrel temperature was adjusted ?80? C., and then, the material to be extruded was fed into a screw extrusion device for low-temperature extrusion processing. The extrudate was mixed with water for saccharification treatment to obtain a glucose liquid. Soluble substances were increased by 3%-30%. The extrudate can be used to produce feed, alcohol, sugar products and their derivatives.

Example 4

[0110] A process for producing fuel alcohol by the extrudate from low-temperature extrusion-multi-enzyme synergistic degradation of starch in dry-process degermed corn.

[0111] First, the raw corn was degermed by a dry process to obtain dry-process degermed corn flour, which was adjusted to have a moisture content ?70%. At the same time, high-temperature-resistant ?-amylase, glucoamylase, fungal ?-amylase and protease were added; the extrusion barrel temperature was adjusted ?80? C., and then, the material to be extruded was fed into a screw extrusion device for low-temperature extrusion processing. The extrudate was mixed with process water. The pH was adjusted, and the liquifying enzyme was added. The temperature was raised to 85? C.-95? C. and maintained for 1 min-15 min, and was then decreased to 55? C.-65? C. The pH was adjusted, and then alcohol-specific complex amyloglucosidase was added for saccharification. The temperature was maintained for 5 min-60 min, and was then decreased to 30? C.-40? C. The pH was adjusted to 3.5-5.5. Fuel alcohol protease and yeast were added to carry out fermentation. After a few hours, the desired alcohol content and starch liquor yield were achieved. Distillation was carried out to obtain alcohol. The technology of this project has the fermentation time of about 45 h-50 h and the alcohol content of 13%, while the prior art requires fermentation time of about 70 h to obtain the alcohol content of 13%.

Example 5

[0112] A process for producing maltose syrup by the extrudate from low-temperature extrusion-multi-enzyme synergistic degradation of dry-process degermed corn.

[0113] First, the raw corn was degermed by a dry process to obtain dry-process degermed corn flour, which was adjusted to have a moisture content ?70%. At the same time, high-temperature-resistant ?-amylase, ?-amylase and pullulanase were added; the extrusion barrel temperature was adjusted ?80? C., and then, the material to be extruded was fed into a screw extrusion device for low-temperature extrusion processing. The extrudate was mixed with process water for producing syrup. The pH was adjusted, and high-temperature-resistant ?-amylase or mesophilic ?-amylase was added. The temperature was raised to 85? C.-95? C. and maintained for 1 min-20 min, and was then decreased to 55? C.-65? C. The above liquefied liquid was poured into a saccharification tank, followed by the pH adjustment and the addition of amyloglucosidase and maltotriohydrolase, for saccharification ?40 h, giving the maltose content of about ?103%.

Example 6

[0114] A process for preparing corn polypeptide by low-temperature extrusion-multi-enzyme synergistic degradation.

[0115] A process for low-temperature extrusion-multi-enzyme synergistic degradation of protein of corn protein powder, wherein the moisture content of the corn protein powder before extrusion was less than 14%, and the powder should have the particle size to pass through an 80-mesh sieve. Then, an appropriate amount of water was added, with the moisture content adjusted to ?70%. At the same time, hemicellulase, neutral protease and pectinase were added in an amount of 0.1 L-5 L or 0.1 kg-5 kg per one ton of raw material for preparing corn polypeptide before extrusion. The pH value of the added water was adjusted to 6.5-8.0. A commercially available single-screw (or twin-screw) extrusion device was used for extrusion processing of the above-mentioned materials. The extrusion barrel temperature was adjusted ?80? C. The extrudate of the corn protein powder added with enzyme preparations for the preparation of corn polypeptide was subject to low-temperature drying at a drying temperature ?70? C., with the moisture content ?15% after drying, and then was pulverized to completely pass through a sieve having the pore diameter of 1.1-2.0 mm for use. Then, conventional extraction was performed to remove soluble non-protein substances (such as saccharides, lipids, etc.), followed by separation (single enzyme and multi-enzyme synergistic hydrolysis or alkali extraction and acid precipitation), purification, ultrafiltration, concentration, spray drying or freeze drying, to produce a powdered corn polypeptide product.

Example 7

[0116] A process for preparing soy isolate protein powder by low-temperature extrusion-multi-enzyme synergistic degradation.

[0117] A process for preparing soy isolate protein powder by low-temperature extrusion-multi-enzyme synergistic degradation of defatted soybean, wherein the defatted soybean meal after the solvent extraction of oil had a moisture content of less than 14% before extrusion, and the powder should have the particle size to pass through an 80-mesh sieve. Then, an appropriate amount of water was added, with the moisture content adjusted to ?70%. At the same time, alkaline protease, hemicellulose and pectinase were added in an appropriate amount of 0.1 L-6 L or 0.1 kg-6 kg per one ton of defatted soybean meal. The pH value of the added water was adjusted to 8-12. A commercially available single-screw (or twin-screw) extrusion device was used. The extrusion barrel temperature was adjusted ?80? C. The extrudate was subject to low-temperature drying at a drying temperature ?70? C., with the moisture content ?15% after drying, and then was pulverized to completely pass through a sieve having the pore diameter of 1.1-2.0 mm for use. The pulverized material of the above extrudate after low-temperature drying was then subject to conventional extraction to remove soluble non-protein substances (such as sacchrides, lipids, etc.), and to separation (single enzyme and multi-enzyme synergistic hydrolysis or alkali extraction and acid precipitation), concentration and spray drying to form a powdered soy isolate protein product.

[0118] The present invention is not limited by the specific embodiments described herein in scope. The foregoing written description is considered to be sufficient to enable those skilled in the art to practice the present invention. In addition to those shown and described herein, various modifications of the present invention will be understood by those skilled in the art from the foregoing description, and the modifications are intended to fall within the scope of the appended claims.