CRYOPRESERVATION DEVICE FOR BIOLOGICAL MATERIAL

Abstract

A cryopreservation device for biological material comprises an outer tube (11) closed in both ends, an inner tube (12) arranged to be filled with the biological material, closed in both ends, and located in the outer tube, and at least one weight element (14) arranged inside the outer tube and outside the inner tube.

Claims

1. A method for storing and transporting semen from an aquatic species, comprising the steps of: a. providing a cryopreservation device consisting of an outer tube, an inner tube having a volume less than 100 L and adapted for fitting inside the outer tube, and two weight elements adapted for fitting in opposite ends of the outer tube, wherein the weight of the weight elements is larger than the buoyancy of the device in a liquid cryogenic agent, b. inserting a volume of semen from an aquatic species in the inner tube, c. sealing the inner tube, d. inserting the inner tube inside the outer tube, e. arranging the two weight elements inside the outer tube and outside the inner tube, wherein the two weight elements are arranged in opposite ends of the outer tube, and sealing the outer tube, f immersing the cryopreservation device in a liquid cryogenic agent, wherein the device is submerged in the liquid cryogenic agent during freezing of the sample and storage of the sample.

2. The method of claim 1, wherein the liquid cryogenic agent is liquid nitrogen.

3. The method of claim 1, where the outer tube is closed by means of welding.

4. The method of claim 1, where the outer tube is closed by means of a sealant.

5. The method of claim 1, where the inner tube is closed by means of welding.

6. The method of claim 1, where the inner tube is closed by means of a sealant.

7. The method of claim 1, wherein the semen comprises zebra fish milt.

Description

[0020] The invention will now be described in more detail by means of an example, and with reference to the accompanying FIGURE.

[0021] FIG. 1 shows schematically an embodiment of the invention.

[0022] In FIG. 1, the cryopreservation device 10 for biological material is shown in assembled state. The cryopreservation device 10 comprises an outer tube 11 closed in both ends by means of sealant 13, an inner tube 12 filled with the biological material (not shown), closed in both ends by means of a sealant 15, and located in the outer tube. The device 10 further comprises two weight elements 14 arranged inside the outer tube and outside the inner tube.

[0023] The outer tube 11 may for example be a medium straw of 0.5 ml, while the inner tube may be a micropipette.