SUGAR COMPOSITION

Abstract

The present invention provides food grade sugar particles comprising sucrose crystals, reducing sugars and polyphenols, wherein the sugar composition comprises about 0 to 0.15 g/100 g reducing sugars and about 20 mg/100 g to about 45 mg/100 g polyphenols and the sugar particles have a glucose based glycaemic index of less than 55.

Claims

1. Food grade sugar particles comprising sucrose crystals, reducing sugars and polyphenols, wherein the sugar particles comprise about 0 to about 0.5 g/100 g reducing sugars; and about 20 mg/100 g to about 45 mg/100 g polyphenols; and wherein the sugar particles have a glucose based glycaemic index of less than 55.

2. The sugar particles of claim 1, wherein a first proportion of the polyphenols are entrained within the sucrose crystals and a second proportion of he polyphenols is distributed on the surfaces of the sucrose crystals.

3. The sugar particles of claim 2, wherein the proportion of the polyphenols entrained within the sugar crystals is about 25% to 30% of the total polyphenol content of the sugar particles.

4. A method for preparing sugar particles comprising washing massecuite to produce sugar particles, wherein the massecuite includes sucrose crystals, polyphenols and reducing sugars, wherein the wash removes an amount of polyphenols and an amount of reducing sugars from the massecuite, wherein the sugar particles comprise about 0 to 0.5 g/100 g reducing sugars and about 20 mg/100 g to about 45 mg/100 g polyphenols and wherein the sugar particles have a glucose based glycaemic index of less than 55.

5. A method for preparing sugar particles according to claim 4, wherein the wash is ceased when the sugar particles comprise 0 to 0.5 g/100 g reducing sugars and less than about 45 mg CE/100 g polyphenols and additional polyphenols are added to the sugar particles to prepare sugar comprising about 20 mg CE/100 g to about 45 mg CE/100 g polyphenols.

6. A method for preparing sugar particles according to claim 4, wherein the wash is ceased when the sugar particles comprise 0 to 0.5 g/100 g reducing sugars and about 20 mg/100 g to about 45 mg/100 g polyphenols and no polyphenols or reducing sugars are either added to or removed from the sugar particles following the wash.

7. The method of claim 4, wherein the massecuite comprises 200-400 mg/100 g polyphenols.

8. The method of claim 4, wherein the amount of polyphenols removed from the massecuite during the wash is 165-380 mg CE/100 g.

9. The method of claim 4, wherein the sugar particles fall within the maximum residue limits for chemicals set out in Schedule 20 of the Australian Food Standards Code in force July 2017.

10. (canceled)

11. The method of claim 4, wherein the sugar particles comprise about 25 mg/100 g to about 35 mg/100 g polyphenols and/or about 98 to about 99.5% w/w sucrose and/or about 0.02% to about 0.6% w/w moisture.

12-18. (canceled)

19. The sugar particles of claim 1, wherein the sugar particles comprise about 0 g/100 g to about 0.2 g/100 g reducing sugars.

20. The sugar particles of claim 1, wherein the sugar particles comprise about 25 mg/100 g to about 35 mg/100 g polyphenols.

21. The sugar particles of claim 1, wherein the sugar particles comprise about 98 to about 99.5% w/w sucrose.

22. The sugar particles of claim 1, wherein the polyphenols include tricin, luteolin and/or apigenin.

23. The sugar particles of claim 1, wherein the sugar particles have a glucose based glycaemic index of about 50.

24. The sugar particles of claim 1, wherein the sugar particles further comprise moisture content of the sugar particles is about 0.02% to about 0.6% w/w.

25. The sugar particles of claim 9, wherein the moisture content of the sugar particles is about 0.1% to about 0.2% wlw.

26. The sugar particles of claim 9, wherein the moisture content of the sugar particles is 0.02% to 0.7% after 6 months storage at room temperature and 40% relative humidity.

27. The sugar particles of claims 9, wherein the increase in the moisture content of the sugar particles is a maximum of 0.3% wlw over 2 years.

28. The sugar particles of claim 1, wherein the sugar particles fall within the maximum residue limits for chemicals set out in Schedule 20 of the Australian Food Standards Code in force July 2017.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0065] FIG. 1 shows a graph of Cl v polyphenol content in mg CE/100 g of sucrose sugars prepared by washing massecuite to various polyphenol contents. This figure shows sugars have low GI at about 22-32 mg CE/100 g polyphenols.

[0066] FIG. 2 graphs moisture (% w/w or mg/100 g), glucose (% w/w or mg/100 g) and fructose (% w/w or mg/100 g) content each separately against polyphenol content in sucrose sugars prepared by washing massecuite to various polyphenol contents. This figure shows that GI increases in sugars with polyphenol content above 32 mg CE/100 g (ie more polyphenols is not better). Without being bound by theory, it is thought that the increase in polyphenol content itself does not increase the GI of the sugar. As the polyphenol content of the sugar increases above about 22-32 mg CE/100 g polyphenols, the reducing sugar content of the sugar also increases and the sugar becomes hygroscopic so moisture content increases. The higher GI of the reducing sugars is then thought to overpower the GI lowering polyphenols and raise the GI of the sugar as a whole.

[0067] FIG. 3 graphs glucose (% w/w or mg/100 g) and fructose (% w/w or mg/100 g) content against polyphenol content in mg CE/100 g for sucrose sugars prepared by washing massecuite to various polyphenol contents. This figure also shows that GI and reducing sugar content increases in sugars with polyphenol content above 32 mg CE/100 g.

[0068] FIG. 4 graphs the sucrose content (% w/w or mg/100 g) and moisture levels (% w/w) against polyphenol content in mg CE/100 g for sucrose sugars prepared by washing massecuite to various polyphenol contents.

[0069] FIG. 5 graphs the polyphenol content in mg CE/100 g versus the conductivity in S/cm of sucrose sugars prepared by washing massecuite to various polyphenol contents. The results show a linear relationship between polyphenol content and conductivity.

[0070] FIG. 6 graphs similar results to FIG. 5 but the graph is limited to a narrower range of polyphenol content.

[0071] FIG. 7 graphs the polyphenol content in mg CE/100 g versus the colour of the sugar in ICUMSA of sucrose sugars prepared by washing massecuite to various polyphenol contents. The results show a linear relationship between polyphenol content and ICUMSA.

[0072] FIG. 8 graphs similar results to FIG. 7 but the graph is limited to a narrower range of polyphenol content.

[0073] FIG. 9 graphs the polyphenol content in mg CE/100 g versus the antioxidant activity (mg GAE/100 g) of sucrose sugars prepared by washing massecuite to various polyphenol contents.

DETAILED DESCRIPTION OF THE EMBODIMENTS

[0074] Reference will now be made in detail to certain embodiments of the invention. While the invention will be described in conjunction with the embodiments, it will be understood that the intention is not to limit the invention to those embodiments. On the contrary, the invention is intended to cover all alternatives, modifications, and equivalents, which may be included within the scope of the present invention as defined by the claims.

[0075] Further aspects of the present invention and further embodiments of the aspects described in the preceding paragraphs will become apparent from the following description, given by way of example.

[0076] All of the patents and publications referred to herein are incorporated by reference in their entirety.

[0077] For purposes of interpreting this specification, terms used in the singular will also include the plural and vice versa.

[0078] One skilled in the art will recognize many methods and materials similar or equivalent to those described herein, which could be used in the practice of the present invention. The present invention is in no way limited to the methods and materials described.

[0079] The inventors of the present invention have developed a method for preparing less refined sugar that retains an efficacious level of phytochemicals including polyphenols and flavonoids. The method avoids the need to add molasses or some other extract from a side product of sugar preparation back onto white refined sugar. Consequently, the method is a more direct way to achieve the desired phytochemical content. As the method is more efficient, it is expected that sugar produced by the method will be a cheaper version of raw sugar than currently available.

[0080] The term reducing sugar refers to any sugar that is capable of acting as a reducing agent. Generally, reducing sugars have a free aldehyde or free ketone group. Glucose, galactose, fructose, lactose and maltose are reducing sugars. Sucrose and trehalose are not reducing sugars.

[0081] The term phytochemical refers generally to biologically active compounds that occur naturally in plants.

[0082] The term polyphenol refers to chemical compounds that have more than one phenol group. There are many naturally occurring polyphenols and many are phytochemicals. Flavonoids are a class of polyphenols. Polyphenols including flavonoids naturally occur in sugar cane. In the context of the present invention the polyphenols that naturally occur in sugar cane are most relevant. Polyphenols in food are micronutrients that are of interest because of the role they are currently thought to have in prevention of degenerative diseases such as cancer, cardiovascular disease or diabetes.

[0083] The term raw sugar refers to a food grade sugar of light brown colour.

[0084] The term refined white sugar refers to fully processed food grade white sugar that is essentially sucrose with minimal reducing sugar content and minimal phytochemicals such as polyphenols or flavonoids.

[0085] The term entrain or entrained refers to incorporating or drawing in. In relation to crystal formation the term refers to incorporating something into the crystal structure or drawing something into the crystal structure. More specifically, in the context of the present invention the term refers to incorporating polyphenols within the sucrose crystals.

[0086] The term molasses refers to a viscous by-product of sugar preparation, which is separated from the crystalised sugar. The molasses may be separated from the sugar at several stages of sugar processing.

[0087] The term massecuite refers to a dense suspension of sugar crystals in the mother liquor of sugar syrup. This is the suspension that remains after concentration of the sugar juice into a syrup by evaporation, crystallisation of the sugar and removal of molasses. The massecuite is the product that is washed in a centrifuge to prepare bulk sugar crystals.

[0088] The term endogenous refers to something originating from within an organism. In the context of the present invention, it refers to something originating from within sugar cane, for example, a phytochemical including monophenol or polyphenol and polysaccharide can be endogenous because the compound originated from within the sugar cane.

[0089] The terms efficacious or effective amount refer to an amount that is biologically effective. In this context, one example is an effective amount of polyphenols in the sugar particles to achieve a low GI sugar. Another example, is an effective lowering of reducing sugar content to achieve minimal hygroscopicity.

[0090] The sugar particles of the present invention can be prepared to food grade quality by methods known to skilled person including using equipment that has covers to prevent external contamination of the sugar particles, for example by bird droppings, the use of magnets to remove iron shavings and other metals and other methods used to prepare food grade sugar.

[0091] Where sugar particles according to the present invention are prepared by ceasing washing of the massecuite before the desired level of polyphenols are washed off, the sugar particles of the present invention will contain a variety of chemicals endogenous to sugar cane, for example monophenolics and polysaccharides. Where the sugar particles of the present invention are prepared by this method, it is still possible to prepare food grade sugar. When refined white sugar is prepared, the massecuite is washed to white and the white bulk sugar is transported to a refinery for further refining. Sugar particles of the present invention can be prepared to the desired specifications and to food grade without needing to send the sugar to a refinery.

[0092] Sugar cane is referred to specifically in this embodiment because sugar beets do not contain the desired levels of polyphenols. Consequently, sugar particles of the present invention cannot be prepared by ceasing washing of sugar beet massecuite at a desired time.

[0093] Sugar particles of the present invention may optionally include additives or extracts such as added flavours, for example maple syrup flavour, colours or additives/extracts to produce additional health, taste, colour or nutritional benefits. Methods for including these additives are known to those skilled in the art.

[0094] Sugar particles of the invention may optionally be cocrystallised or agglomerated. Methods for performing these processed are known to those skilled in the art.

[0095] ICUMSA is a sugar colour grading system. Lower ICUMSA values represent less colour. ICUMSA is measured at 420 nm by a spectrophotometric instrument such as a Metrohm NIRS XDS spectrometer with a ProFoss analysis system. Currently, sugars considered suitable for human consumption, including refined granulated sugar, crystal sugar, and consumable raw sugar (ie brown sugar), have ICUMSA scores of 45-800. Sugars with scores above 800 are currently used for cosmetics or other non-edible purposes, but require further processing to be fit for human consumption. Consequently, the food grade sugars of the invention with ICUMSA of 500 to 2000 ICUMSA, about 750 to 1800 ICUMSA, about 1000 to 1500 ICUMSA are unexpected.

[0096] The sugar particles of the present invention may optionally be prepared using the methods and systems described in Australian Provisional Patent Application No 2016902957 filed on 27 Jul. 2016 with the title Process for sugar production.

REFERENCES

[0097] Jaffee, W. R., Sugar Tech (2012) 14:87-94

[0098] Joint FAO/WHO Report. Carbohydrates in Human Nutrition. FAO Food and Nutrition. Paper 66. Rome: FAO, 1998.

[0099] Kim, Dae-Ok, et al (2003) Antioxidant capacity of phenolic phytochemicals from various cultivars of plums. Food Chemistry, 81, 321-26.

[0100] Wolever TMS et al. Determination of the glycemic index values of foods: an interlaboratory study. European Journal of Clinical Nutrition 2003;57:475-482.

[0101] A copy of each of these is incorporated into this specification by reference.

EXAMPLES

Example 1

Preparation of Sugar Samples for GI Testing

[0102] Sugar 1 was prepared at a sugar mill by processing sugar cane to massecuite. The massecuite was washed until it had 22-32 mg/100 g polyphenol content. One method of achieving sugar with 22-32 mg/100 g polyphenol content is to wash the massecuite in batches and wash each batch a different length of time. The polyphenol content of each washed batch can be analysed as set out in Example 2. The batch with the appropriate polyphenol content can then be selected. It will be understood by the person skilled in the art that each time massecuite is prepared its components vary. Therefore, there is no single set of wash conditions, eg time, spin and water flow, that will always result in a sugar with the desired polyphenol content. The appropriate wash time will vary depending on the components in the massecuite that is being washed.

TABLE-US-00001 TABLE 1 Sugar samples Polyphenols Moisture Sample Sugar content (mg CE/100 g) (%) Standard glucose 0 Control sugar 99.9% sucrose 0 0.075 (refined white 0% glucose sugar) 0% fructose Sugar 1 95.2% sucrose 26.5 0.36 0.11% glucose 0.09% fructose Sugar 2 89.5% sucrose 60.9 0.65 1.42% glucose 1.55% fructose

Example 2

Analysis of Polyphenol Content in Sugar

[0103] 40 g of sugar sample was accurately weighed into a 100 ml volumetric flask. Approximately 40 ml of distilled water was added and the flask agitated until the sugar was fully dissolved after which the solution was made up to final volume with distilled water. The polyphenol analysis was based on the Folin-Ciocalteu method (Singleton 1965) adapted from the work of Kim et al (2003). In brief, a 50 L aliquot of appropriately diluted raw sugar solution was added to a test tube followed by 650 L pf distilled water. A 50 L aliquot of Folin-Ciocalteu reagent was added to the mixture and shaken. After 5 minutes, 500 L of 7% Na.sub.2CO.sub.3 solution was added with mixing. The absorbance at 750 nm was recorded after 90 minutes at room temperature. A standard curve was constructed using standard solutions of catechin (0-250 mg/L). Sample results were expressed as milligrams of catechin equivalent (CE) per 100 g raw sugar. The absorbance of each sample sugar was determined and the quantity of polyphenols in that sugar determined from the standard curve.

[0104] Where the sugar is a less refined sugar prepared by a limited wash, an alternative method for analysis of the polyphenol content is to measure the amount of tricin in a sample using near-infra red spectroscopy (NIR). In these circumstances, the amount of tricin is proportional to the total polyphenols. Further information on this method is available in Australian Provisional Patent Application No 2016902957 filed on 27 Jul. 2016 with the title Process for sugar production.

Example 3

Analysis of the Reducing Sugar Content in Sugar

[0105] There are several qualitative tests that can be used to determine reducing sugar content in a sugar product. Copper (II) ions in either aqueous sodium citrate or in aqueous sodium tartrate can be reacted with the sugar. The reducing sugars convert the copper(II) to copper(I), which forms a copper(I) oxide precipitate that can be quantified.

[0106] An alternative is to react 3,5-dinitrosalicylic acid with the sugar. The reducing sugars will react with this reagent to form 3-amino-5-nitrosalicylic acid. The quantity of 3-amino-5-nitrosalicylic acid can be measured with spectrophotometry and the results used to quantify the amount of reducing sugar present in the sugar product.

Example 4

GI Testing

[0107] The GI testing was conducted using internationally recognised GI methodology (see the Joint FAO/WHO Report), which has been validated by results obtained from small experimental studies and large multi-centre research trials (see Wolever et al 2003). The experimental procedures used in this study were in accordance with international standards for conducting ethical research with humans approved by the Human Research Ethics Committee of Sydney University.

[0108] Experimental Procedures

[0109] Using standard methodology to determine a food's GI value, a portion of the food containing between 10 and 50 grams of available carbohydrate is fed to 10 healthy people the morning after they have fasted for 10-12 hours overnight. A fasting blood sample is first obtained from each person and then the food is consumed, after which additional blood samples are obtained at regular intervals during the next two hours. In this way, it's possible to measure the total increase in blood sugar produced by that food over a two-hour period. The two-hour blood glucose (glycaemic) response for this test food is then compared to the two-hour blood glucose response produced by the same amount of carbohydrate in the form of pure glucose sugar (the reference food: GI value of glucose =100%). Therefore, GI values for foods and drinks are relative measures (ie they indicate how high blood sugar levels rise after eating a particular food compared to the very high blood sugar response produced by the same amount of carbohydrate in the form of glucose sugar). Equal-carbohydrate portions of test foods and the reference food are used in GI experiments, because carbohydrate is the main component in food that causes the blood's glucose level to rise.

[0110] The night before each test session, the subjects ate a regular low-fat evening meal based on a carbohydrate-rich food, other than legumes, and then fasted for at least 10 hours overnight. The subjects were also required to avoid alcohol and unusual levels of food intake and physical activity for the whole day before each test session.

[0111] Measurement of the Subjects' Blood Glucose Responses

[0112] For each subject, the concentration of glucose in each of the eight whole blood samples collected from them during each test session was analysed in duplicate using a HemoCue B-glucose photometric analyser employing a glucose dehydrogenase/mutarotase enzymatic assay (HemoCue AB, ngelholm, Sweden). Each blood sample was collected into a plastic HemoCue cuvette containing the enzymes and reagents for the blood glucose assay and then placed into the HemoCue analyser while the enzymatic reaction took place. Therefore, each blood sample was analysed immediately after it was collected.

[0113] For each of the 10 subjects, a two-hour blood glucose response curve was constructed for each of their test sessions using the average blood glucose concentrations for each of their eight blood samples. The two fasting blood samples were averaged to provide one baseline glucose concentration. The area under each two-hour blood glucose response curve (AUC) was then calculated in order to obtain a single number, which indicates the total increase in blood glucose during the two-hour test period in that subject as a result of ingesting that food. A glycaemic index (GI) value for each test sugar was then calculated for each subject by dividing their two-hour blood glucose AUC value for the test food by their average two-hour blood glucose AUC value for the reference food and multiplying by 100 to obtain a percentage score.

GI value (%)=Blood glucose AUC value for the test food100

[0114] Average AUC value for the equal carbohydrate portion of the reference food

[0115] Due to differences in body weight and metabolism, blood glucose responses to the same food or drink can vary between different people. The use of the reference food to calculate GI values reduces the variation between the subjects' blood glucose results to the same food arising from these natural differences. Therefore, the GI value for the same food varies less between the subjects than their glucose AUC values for this food.

TABLE-US-00002 TABLE 2 GI for samples prepared in example 1 Sample GI (SEM) GI category Reference 100 0 High GI Control sugar 68 3 Medium GI Sugar 1 53 4 Low GI Sugar 2 70 4 High GI

Example 5

Relationship Between GI and Polyphenol, Glucose, Fructose and Moisture Content

[0116] Increasing glucose and fructose in low GI sugar can affect the GI of sugar. In many unrefined sugars, as sucrose content decreases reducing sugar content increases. The increase in reducing sugars can increase the GI of the unrefined sugar. This effect is counterintuitive and unexpected. Most consumers understand that less refined products are healthier or better for you. However, that is not necessarily the case for unrefined sugar. The healthiest sugars minimise reducing sugar content without refining our all the polyphenols responsible for the low GI. There is a sweet spot in the extent to which sugar is refined where GI remains low. The inventors of the present invention have researched less refined sugars by varying the extent of the massecuite washing. Too much washing removed the majority of polyphenol content and increased the GI. Too little washing resulted in a higher reducing sugar content, which is thought to overpower the GI lowering effect of the polyphenols and increase the GI of the sugar.

[0117] The low GI sweet spot was demonstrated by graphing the results of the sugars in Table 3 below. This graph demonstrates that at least 22 mg CE/100 mg sucrose needs to be retained during sugar processing to produce a low GI sugar. If additional polyphenols are present but reducing sugars are too high then GI effect is removed. Respraying molasses back onto refined white and less refined raw sugars to produce a brown sugar may therefore not be an effective strategy to reduce GI.

TABLE-US-00003 TABLE 3 Example sugars Polyphenols Sucrose Glucose Fructose Moisture mg/100 g GI (%) SE (%) (%) (%) (%) 0 68 3 99.9 0 0 0.075 15-19 66 3 99.1 0.04 0.04 0.09 22-32 50 5 95.2 0.11 0.09 0.36 60-70 70 4 89.5 0.17 0.21 0.65

[0118] FIG. 1 shows a graph of GI v polyphenol content of these sugars. This figure shows sugars have low GI at about 22-32 mg CE/100 g polyphenols. FIG. 2 graphs moisture, glucose and fructose content each separately against polyphenol content. FIG. 3 graphs glucose and fructose content against polyphenol content for these example sugars. FIGS. 2 and 3 illustrate why GI is higher in sugars with higher polyphenol content (ie sugars that would otherwise be expected to remain low GI). As the polyphenol content increases above about 22-32 mg CE/100 g polyphenols, the reducing sugar content of the sugar increases, the sugar becomes hygroscopic so moisture content increases and the higher GI of the glucose and fructose begin to raise the GI of the sugar as a whole despite the GI lowering polyphenols.

Example 6

Washing of Massecuite to Desired Polyphenol Content

[0119] Ten massecuite samples were prepared at two different sugar mills designated Mill 1 and Mill 2. The polyphenol content of each sample was determined (see Example 2). The massecuite samples were washed until they were the depth of colour that is associated with the desired polyphenol content (ie roughly 500 to 2000 ICUMSA) and the polyphenol content measured. The results are in Table 4 below. The skilled person will understand that if the polyphenol content remains too high after the wash, a second wash is possible. The results for each sample are below. The polyphenol content of several of the samples below is too low. Those samples would have to be discarded. It is usual for some sugars prepared at a sugar mill to not meet specifications for various reasons.

TABLE-US-00004 TABLE 4 Example sugars Polyphenol content Massecuite Less refined removed during polyphenols sugar polyphenols massecuite washing Sample (mg CE/100 g) (mg CE/100 g) (mg CE/100 g) Mill 1 - 1 316.8 23.1 293.7 Mill 1 - 2 312 24.3 287.7 Mill 1 - 3 287.6 25.8 261.8 Mill 1 - 4 291.8 18.6 273.2 Mill 1 - 5 314.6 20.5 294.1 Mill 1 - 6 301.8 24.1 277.7 Mill 1 - 7 277.3 17.1 260.2 Mill 1 - 8 262.3 19.5 242.8 Mill 1 - 9 305.4 18.2 287.2 Mill1 - 10 314.7 23.6 291.1 Mill 2 - 1 283 24 259 Mill 2 - 2 267.2 24.2 243 Mill 2 - 3 246.4 24.6 221.8 Mill 2 - 4 262.2 20.2 242 Mill 2 - 5 270.8 30.2 240.6 Mill 2 - 6 282.6 25 257.6 Mill 2 - 7 269.1 23.5 245.6 Mill 2 - 8 256.8 21.2 235.6 Mill 2 - 9 268.9 22.9 246 Mill 2 - 10 276 21.6 254.4

Example 7

Relationship Between Polyphenol Content and Conductivity/ICUMSA

[0120] Further sugar particles were prepared as described in Example 6. Their polyphenol content, conductivity and ICUMSA were measured and the linear relationship between the two confirmed. The results are in Table 5 below.

[0121] ICUMSA was measured with a Metrohm NIRS XDS spectrometer with a ProFoss analysis system. Conductivity was measured under standard conditions by the conductivity meter InPro 7000-VP Conductivity sensor by Mettler Toledo.

TABLE-US-00005 TABLE 5 Features of sugars prepared Polyphe- Conduc- Polyphe- Conduc- nols (mg tivity nols (mg tivity CE/100 g) (S/cm) ICUMSA CE/100 g) (S/cm) ICUMSA 15.88 101 665 31.88 210 1390 16.57 110 620 32.19 220 1560 17.73 100 625 33.76 240 1660 17.87 120 710 34.85 270 1485 18.10 110 915 34.86 250 1880 18.43 110 815 35.24 250 1510 18.53 130 915 37.71 300 1695 18.87 100 760 38.13 290 1600 19.45 120 785 39.58 280 1690 19.71 130 870 41.72 310 1750 20.71 130 780 41.97 300 1725 20.97 150 750 84.32 650 3715 21.31 140 960 26.7 180 1520 21.44 150 960 35 250 2240 21.88 130 865 26.6 200 1740 22.26 120 715 29.1 224 1870 22.47 180 1320 81 614 4090 22.53 130 750 65.9 500 3240 22.84 130 735 64 487 3170 24.02 160 1100 52.8 430 2520 24.05 180 1045 29.3 213 1470 24.15 140 990 34.8 300 1610 24.38 170 985 33.3 278 1650 24.58 160 1050 34.8 280 1700 24.84 170 1100 32 257 1530 25.07 180 1055 28.5 202 1440 25.13 210 1235 39.1 289 1980 25.47 170 1255 35 276 1870 25.56 180 1175 35.1 279 1880 25.59 180 1140 32.4 267 1740 25.80 160 1120 37.9 289 1970 25.82 190 930 29.9 199 1520 26.01 180 1080 29.7 202 1490 26.26 170 1145 31.3 213 1740 26.50 160 1065 76 600 4030 26.68 190 1170 28 189 1460 26.75 180 1175 66 513 3255 26.98 190 1100 32 216 1721 27.05 190 1130 28.26 210 1280 28.46 210 1250 28.53 210 1080 29.02 210 1230 29.11 190 1135 29.96 200 1295 30.67 200 1320 31.47 210 1495 31.58 210 1545

[0122] The relationship between the polyphenol content of the sugar prepared and the ICUMSA/conductivity of that sugar was graphed to show a linear relationship between polyphenol content and ICUMSA/conductivity. The graphs are in FIGS. 5 to 8.

Example 8

Relationship Between Polyphenol Content and Antioxidant Activity

[0123] Further sugar particles were prepared as described in Example 6 and various parameters of the sugar particles measured. The results are in Table 6 below. Some of the methods of measurement are as described elsewhere. Methods for measuring antioxidant activity and t-Aconitic acid are standard and well known in the art.

TABLE-US-00006 TABLE 6 Features of prepared sugars Total Antioxidant t- phenolics activity Aconitic Reducing Sample (mg (mg acid Sucrose Sugars Colour No CE/100 g) GAE/100 g) (mg/100 g) (mg/100 g) (mg/100 g) ICUMSA 1 26.7 8.5 32.4 98.94 0.23 1520 2 35 10.7 37.8 98.72 0.25 2240 3 26.6 8.2 25 98.93 0.23 1740 4 29.1 9.1 34.3 98.87 0.21 1870 6 81 24.6 89.8 97.35 0.65 4090 7 65.9 21.2 81.7 97.78 0.56 3240 8 64 20.1 76.4 97.75 0.54 3170 9 52.8 16.9 59.9 98.21 0.41 2520 10 29.3 9.3 36.7 99.06 0.18 1470 11 34.8 11 40.9 99 0.21 1610 12 33.3 10.6 39.5 98.96 0.2 1650 13 34.8 11.1 44.4 98.92 0.23 1700 14 32 10.3 36.2 99.04 0.2 1530 15 28.5 9.3 34.1 99.1 0.18 1440 16 39.1 12.1 44.5 98.84 0.26 1980 17 35 11.2 39.8 99.01 0.21 1870 18 35.1 11.1 38.8 98.91 0.22 1880 19 32.4 10.8 40.1 99.04 0.2 1740 20 31.7 10.3 38.3 99.02 0.2 1690 21 34.2 11 38.8 99 0.22 1770 22 37.9 12.1 42 98.86 0.26 1970 23 29.9 9.7 34.6 99.15 0.18 1520 24 29.7 9.4 36.2 99.11 0.18 1490 25 31.3 10.4 39.2 98.18 0.22 1740 26 76 25 84 4030 27 28 10 32 1460 28 66 21 77 98 0.540 3255 29 32 10 38 99 0.214 1721

[0124] The relationship between the polyphenol content of the sugar prepared and the antioxidant activity of that sugar was graphed. The graph is in FIG. 9.

Example 9

Relationship Between Polyphenol Content and Mineral Content

[0125] The sugar particles from Example 8 were also analysed to determine the amounts of various minerals. The results are in Table 7 below. Some of the methods of measurement are as described elsewhere. Methods for measuring mineral content are standard and well known in the art.

TABLE-US-00007 TABLE 7 Content of example sugars Total Sam- phenolics Element ple (mg (mg/kg) No CE/100 g) Na K Ca Mg Fe PO.sub.4 SO.sub.4 Cl 1 26.7 67 427 171 28 35 17 617 100 2 35 81 555 213 44 32 43 627 122 3 26.6 51 390 145 29 52 22 530 77 4 29.1 55 527 159 32 21 23 646 112 6 81 97 1810 453 119 19 43 737 584 7 65.9 31 1535 367 111 29 35 751 435 8 64 70 1463 419 92 24 19 704 418 9 52.8 64 1106 308 74 74 28 680 277 10 29.3 95 557 168 38 15 14 672 152 11 34.8 45 606 182 42 12 15 735 182 12 33.3 80 562 407 52 13 12 704 173 13 34.8 82 660 270 59 14 15 652 158 14 32 81 577 149 52 14 21 680 144 15 28.5 66 506 135 44 16 14 598 117 16 39.1 47 645 206 57 17 28 590 185 17 35 55 602 292 58 17 33 768 161 18 35.1 41 581 203 54 14 17 766 145 19 32.4 83 575 189 57 14 14 884 121 20 31.7 111 636 236 63 15 27 813 127 21 34.2 39 595 194 59 14 19 801 133 22 37.9 55 601 195 64 51 22 804 129 23 29.9 49 552 183 53 16 19 881 109 24 29.7 18 493 139 54 13 14 767 101 25 31.3 48 596 176 66 15 19 816 129 26 76 51 1189 323 134 23 47 599 419 27 28 26 331 207 45 11 20 540 85 28 66 66 1479 387 99 37 31 718 429 29 32 62 562 201 50 21 20 718 134