MEDIUM AND METHOD FOR CULTURING ANTRODIA CINNAMOMEA

Abstract

A medium for culturing Antrodia cinnamomea includes a carbon source, a nitrogen source, vitamin A, agar and a solvent. The medium includes 8-16 grams/liter of the carbon source, 0.4-1.6 grams/liter of the nitrogen source and 4 grams/liter of vitamin A. Moreover, a method for culturing Antrodia cinnamomea includes inoculating the medium with mycelia of Antrodia cinnamomea. The mycelia of Antrodia cinnamomea inoculated on the medium is cultured at 20-27 C. for 28-50 days.

Claims

1. A medium for culturing Antrodia cinnamomea, comprising: 0.8-1.6% (w/v) of a carbon source; 0.04-0.16% (w/v) of a nitrogen source; 0.4% (w/v) of vitamin A; agar; and a solvent until 100% (w/v).

2. The medium as claimed in claim 1, wherein the carbon source is selected from the group consisting of malt extract, glucose, fructose, galactose, lactose, starch, cellulose, sucrose and combination therof.

3. The medium as claimed in claim 1, wherein the nitrogen source is selected from the group consisting of peptone, yeast extract, whey protein, tryptone, amino acids, urea, ammonium acetate, ammonium chloride, ammonium nitrate, potassium nitrate, sodium nitrate and combination therof.

4. The medium as claimed in claim 1, wherein the medium comprises 0.8% (w/v) of malt extract, 0.8% (w/v) of glucose and 0.04% (w/v) of peptone.

5. The medium as claimed in claim 1, wherein the medium comprises 0.8% (w/v) of malt extract, 0.08% (w/v) of peptone and 0.08% (w/v) of yeast extract.

6. A method for culturing Antrodia cinnamomea, comprising: inoculating a medium with mycelia of Antrodia cinnamomea, wherein the medium comprises: 0.8-1.6% (w/v) of a carbon source, 0.04-0.16% (w/v) of a nitrogen source, 0.4% (w/v) of vitamin A, agar and a solvent until 100% (w/v); and culturing the mycelia of Antrodia cinnamomea inoculated on the medium at 20-27 C. for 28-50 days.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0012] The patent or application file contains at least one drawing executed in color. Copies of this patent or patent application publication with color drawing(s) will be provided by the Office upon request and payment of the necessary fee.

[0013] FIG. 1 depicts a chemical structure of vitamin A.

[0014] FIG. 2a depicts photographs illustrating growth status of mycelia of Antrodia cinnamomea grown on medium according to a first embodiment of the present invention.

[0015] FIG. 2b depicts a bar chart illustrating average diameter of the mycelia of Antrodia cinnamomea grown on medium according to the first embodiment of the present invention.

[0016] FIG. 3a depicts photographs illustrating growth status of mycelia of Antrodia cinnamomea grown on medium according to a second embodiment of the present invention.

[0017] FIG. 3b depicts a bar chart illustrating average diameter of the mycelia of Antrodia cinnamomea grown on medium according to the second embodiment of the present invention.

DETAILED DESCRIPTION OF THE INVENTION

[0018] Vitamin A with a chemical structure shown in FIG. 1 is one of the fat-soluble vitamins. Vitamin A does not easily lose due to metabolism, and can be kept in cells, which is appreciated by a person having ordinary skill in the art. Detail description is not given to avoid redundancy.

[0019] Specifically, vitamin A can be used as an additive in a medium for culturing Antrodia cinnamomea. That is, 1 liter of the medium includes 4 grams of vitamin A. Therefore, when the medium is used to culture mycelia of Antrodia cinnamomea, as an example, Antrodia cinnamomea strains with BCRC number of BCRC 35396, BCRC 35716, BCRC 36401 and BCRC 37848, vitamin A in the medium can be uptaken by the mycelia of Antrodia cinnamomea and the mycelia of Antrodia cinnamomea in red color can thus be obtained.

[0020] Moreover, the medium can further include a carbon source as well as a nitrogen source for growth of Antrodia cinnamomea. As an example, the medium can include 0.8-1.6% (w/v) of a carbon source and 0.04-0.16% (w/v) of a nitrogen source. That is, 8-16 grams of the carbon source, 0.4-1.6 grams of the nitrogen source and 4 grams of vitamin A are mixed, and a solvent, such as distilled water, is added until the total volume is 1 liter. The carbon source can be selected from the group consisting of malt extract, glucose, fructose, galactose, lactose, starch, cellulose, sucrose and combination therof. The nitrogen source can be selected from the group consisting of peptone, yeast extract, whey protein, tryptone, amino acids, urea, ammonium acetate (CH.sub.3COONH.sub.4), ammonium chloride (NH.sub.4Cl), ammonium nitrate (NH.sub.4NO.sub.3), potassium nitrate (KNO.sub.3), sodium nitrate (NaNO.sub.3) and combination therof. In addition, the medium can also include proper amount of agar, and thus the medium can be formed in a solid state and can be used in the solid state fermentation.

[0021] The medium according to a first embodiment of the present invention includes 0.8% (w/v) of malt extract, 0.8% (w/v) of glucose, 0.04% (w/v) of peptone, 0.4% (w/v) of vitamin A, 0.8% (w/v) of agar and 97.16% (w/v) of water. Besides, the medium according to a second embodiment of the present invention includes 0.8% (w/v) of malt extract, 0.08% (w/v) of peptone, 0.08% (w/v) of yeast extract, 0.4% (w/v) of vitamin A, 0.8% (w/v) of agar and 97.84% (w/v) of water.

[0022] Therefore, the medium according to the first and the second embodiments of the present invention can be applied in a method for culturing Antrodia cinnamomea. The medium is inoculated with the mycelia of Antrodia cinnamomea, and the mycelia of Antrodia cinnamomea inoculated on the medium is cultured at 20-27 C. for 28-50 days. The mycelia of Antrodia cinnamomea in red color can thus be obtained.

[0023] To evaluate the medium with vitamin A as the additive can be used to obtain the mycelia of Antrodia cinnamomea in red color, the mycelia of Antrodia cinnamomea is inoculated on the medium listed in TABLE 1, and cultured at 20-27 C. for 35 days. Growth status and average diameter of colonies on the medium on days 0, 7, 14, 28 and 35 are shown in FIGS. 2a and 2b.

TABLE-US-00001 TABLE 1 Medium Malt Vitamin Growth status extract Glucose Peptone A Biomass Growth rate Group (%) (%) (%) (%) (mg) (R.sup.2) A0 0.8 0.8 0.04 0 3.4 0.2174 R.sup.2 = 0.9598 A1 0.8 0.8 0.04 0.4 6.85 0.1767 R.sup.2 = 0.8968 A2 0.8 0.8 0.04 0.8 4.49 0.0398 R.sup.2 = 0.9251

[0024] Referring to FIG. 2a, the mycelia of Antrodia cinnamomea obtained by using the medium of groups A0-A2 have color with hexadecimal color codes of #FFC78E, #FF5809 and #FFEEDD, respectively. That is, the mycelia of Antrodia cinnamomea obtained by using the medium of group A1 (with vitamin A in the concentration of 0.4% (w/v)) is redder than the mycelia of Antrodia cinnamomea obtained by using either the medium of group A0 (without vitamin A) or the medium of group A2 (with vitamin A in a concentration of 0.8% (w/v)). Moreover, referring to TABLE 1, on day 35, biomass of the mycelia of Antrodia cinnamomea obtained by using the medium of group A1 significantly increases compared to biomass of the mycelia of Antrodia cinnamomea obtained by using the medium of group A0 or A2. Referring to FIG. 2b, the mycelia of Antrodia cinnamomea obtained by using the medium of group A1 has similar diameter with the mycelia of Antrodia cinnamomea obtained by using the medium of group A0.

[0025] In addition, the mycelia of Antrodia cinnamomea is inoculated on the medium listed in TABLE 2, and cultured at 20-27 C. for 35 days. Growth status and average diameter of colonies on the medium on days 0, 7, 14, 28 and 35 are shown in FIGS. 3a and 3b.

TABLE-US-00002 TABLE 2 Medium Malt Vitamin Growth status extract Glucose Peptone A Biomass Growth rate Group (%) (%) (%) (%) (mg) (R.sup.2) B0 0.8 0.08 0.08 0 2.60 0.1893 R.sup.2 = 0.9763 B1 0.8 0.08 0.08 0.4 4.35 0.2078 R.sup.2 = 0.9356 B2 0.8 0.08 0.08 0.8 5.40 0.0908 R.sup.2 = 0.9693

[0026] Referring to FIG. 3a, the mycelia of Antrodia cinnamomea obtained by using the medium of groups B0-B2 have color with hexadecimal color codes of #FFD1A4, #FF8040 and #FFEEDD, respectively. That is, the mycelia of Antrodia cinnamomea obtained by using the medium of group B1 (with vitamin A in the concentration of 0.4% (w/v)) is redder than the mycelia of Antrodia cinnamomea obtained by using either the medium of group B0 (without vitamin A) or the medium of group B2 (with vitamin A in a concentration of 0.8% (w/v)). Moreover, referring to TABLE 2, on day 35, biomass of the mycelia of Antrodia cinnamomea obtained by using the medium of group B1 significantly increases compared to biomass of the mycelia of Antrodia cinnamomea obtained by using the medium of group B0 or B2. Referring to FIG. 3b, the mycelia of Antrodia cinnamomea obtained by using the medium of group B1 has similar diameter with the mycelia of Antrodia cinnamomea obtained by using the medium of group B0.

[0027] Accordingly, the mycelia of Antrodia cinnamomea grown on the medium can uptake vitamin A in the medium and the mycelia of Antrodia cinnamomea in red color can be obtained. Therefore, the consumer's willingness to purchase the mycelia of Antrodia cinnamomea increases.

[0028] Although the invention has been described in detail with reference to its presently preferable embodiment, it will be understood by one of ordinary skill in the art that various modifications can be made without departing from the spirit and the scope of the invention, as set forth in the appended claims.