REACTIVE GAS, REACTIVE GAS GENERATION SYSTEM AND PRODUCT TREATMENT USING REACTIVE GAS

Abstract

A method of treating a product or surface with a reactive gas, comprises producing the reactive gas by forming a high-voltage cold plasma (HVCP) from a working gas; transporting the reactive gas at least 5 cm away from the HVCP; followed by contacting the product or surface with the reactive gas. The HVCP does not contact the product or surface.

Claims

1. A method of treating a product with a reactive gas, comprising: producing the reactive gas by forming a high-voltage cold plasma (HVCP) from a working gas; transporting the reactive gas at least 5 cm away from the HVCP; followed by contacting the product with the reactive gas; wherein the HVCP does not contact the product.

2. The method of claim 1, wherein the transporting is transporting the reactive gas at least 1 meter away from the HVCP.

3. The method of claim 1, wherein the product is not sealed or substantially sealed inside a package during the contacting.

4. The method of claim 1, wherein: the transporting is transporting the reactive gas at least 1 meter away from the HVCP, and the product is not sealed or substantially sealed inside a package during the contacting.

5. The method of claim 4, wherein the transporting is transporting the reactive gas at least 3 meter away from the HVCP and the product is medically sterilized by the contacting.

6. The method of claim 5, wherein the product comprises food.

7. The method of claim 5, wherein the product comprises grain.

8. The method of claim 5, further comprising removing the reactive gas from contact with the product, after 1 second to 12 hours.

9. The method of claim 5, wherein the reactive gas comprises at least one reactive or excited species other than ozone.

10. The method of claim 5, wherein the working gas comprises MA65.

11. A method of reducing mycotoxins on fruit or seeds, comprising: producing a reactive gas by forming a high-voltage cold plasma (HVCP) from a working gas; transporting the reactive gas at least 3 meters away from the HVCP; followed by contacting the fruit or seeds with the reactive gas.

12. The method of claim 11, wherein the fruit or seeds are medically sterilized by the contacting.

13. The method of claim 11, wherein the working gas comprises MA65.

14. The method of claim 11, wherein the reactive gas comprises at least one reactive or excited species other than ozone.

15. The method of claim 11, wherein the fruit or seeds are whole corn or whole wheat.

16. The method of claim 11, wherein the contacting is carried out with the fruit or seeds in a fluidized bed.

17. The method of claim 11, further comprising removing the reactive gas from contact with the fruit or seeds, after 35 minutes to 12 hours.

18. The method of claim 11, wherein prior to the method, the fruit or seeds contain too much mycotoxin for use as human food by US standards, and after the contacting the fruit or seeds is suitable for use as human food by US standards.

19. The method of claim 11, wherein the contacting is carried out with the fruit or seeds moving on a conveyor.

20. The method of claim 11, wherein the contacting is carried out in a treatment chamber having a volume of at least 1 cubic meter.

21. The method of claim 11, wherein an amount of mycotoxin present on the fruit or seeds is reduced by at least 50%.

22. The method of claim 11, wherein an amount of mycotoxin present on the fruit or seeds is reduced by at least 90%.

23. A method of medically sterilizing a surface, comprising: producing the reactive gas by forming a high-voltage cold plasma (HVCP) from a working gas; and contacting the surface with the reactive gas; wherein the HVCP does not contact the surface, and the surface is the surface of an enclosed space, or equipment in an enclosed space, where the enclosed space has a volume of at least 8 cubic meter.

24. The method of claim 23, wherein at least a portion of the surface is at least 1 meter away from the HVCP.

25. The method of claim 23, wherein at least a portion of the surface is at least 3 meter away from the HVCP.

26. The method of claim 25, further comprising removing the reactive gas from contact with the surface, after 1 second to 12 hours.

27. The method of claim 25, wherein the working gas comprises MA65.

28. The method of claim 25, wherein the reactive gas comprises at least one reactive or excited species other than ozone.

29. The method of claim 25, wherein the surface is the inside surface of a room in a hospital.

30. A method of treating a product or surface with a reactive gas, comprising: providing a container having stored reactive gas produced by forming a high-voltage cold plasma (HVCP) from a working gas; and contacting the product or surface with the reactive gas; wherein the reactive gas comprises at least one reactive or excited species other than ozone.

31. A system for treating a product or surface with a reactive gas, comprising: (1) A dielectric barrier discharge (DBD) system, and (2) a treatment chamber, fluidly connected to the DBD system, wherein the treatment chamber has a volume of at least 1 cubic meter.

32. The system of claim 31, wherein the DBD system comprises: (i) a first electrode fully enclosed by a dielectric, and (ii) a second electrode, electrically grounded, (iii) an alternating current (AC) voltage source, wherein a plenum is present between the first and second electrodes.

33. The system of claim 31, wherein the DBD system comprises: (a) a plurality of first electrode, and (b) a plurality of second electrodes, each between two first electrodes, wherein there are the same number of first electrodes as second electrodes, or one more first electron than second electrodes (c) at least one dielectric barrier between each adjacent first electrode and second electrode, wherein each adjacent first electrode and second electrode forms a plenum.

34. The system of claim 31, wherein the distance between the DBD system and the treatment chamber is at least 3 meters.

35. The system of claim 31, wherein the treatment chamber has a volume of at least 8 cubic meters.

36. The system of claim 31, further comprising a conveyor inside the treatment chamber.

37. The system of claim 31, further comprising a fan, for transporting a reactive gas from the DBD system to the treatment chamber.

38. The method of claim 4, wherein the transporting is transporting the reactive gas at least 3 meter away from the HVCP and the product is canning sterilized by the contacting.

39. The method of claim 4, wherein the transporting is transporting the reactive gas at least 3 meter away from the HVCP and the product is Salmonella pasteurized by the contacting.

40. The method of claim 4, wherein the transporting is transporting the reactive gas at least 3 meter away from the HVCP and the product is Listeria pasteurized by the contacting.

41. The method of claim 4, wherein the transporting is transporting the reactive gas at least 3 meter away from the HVCP and the product is E. coli pasteurized by the contacting.

42. The method of claim 11, wherein the fruit or seeds are canning sterilized by the contacting.

43. The method of claim 11, wherein the fruit or seeds are Salmonella pasteurized by the contacting.

44. The method of claim 11, wherein the fruit or seeds are Listeria pasteurized by the contacting.

45. The method of claim 11, wherein the fruit or seeds are E. coli pasteurized by the contacting.

46. The method of claim 11, wherein prior to the method, the fruit or seeds contain too much mycotoxin for use as human food by EU standards, and after the contacting the fruit or seeds is suitable for use as human food by EU standards.

47. The method of claim 11, wherein prior to the method, the fruit or seeds contain too much mycotoxin for use as animal feed by US standards, and after the contacting the fruit or seeds is suitable for use as animal feed by US standards.

48. The method of claim 11, wherein prior to the method, the fruit or seeds contain too much mycotoxin for use as animal feed by EU standards, and after the contacting the fruit or seeds is suitable for use as animal feed by EU standards.

49. The method of claim 47, wherein the fruit or seeds are grains.

50. The method of claim 48, wherein the fruit or seeds are grains.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0027] The following figures are provided to help illustrate the products, devices and methods of the application, but other variations and configurations are possible. The figures are not drawn to scale, with the size of some parts increased or decreased for clarity.

[0028] FIGS. 1A, 1B, 1C, 1D, 1E and 1F are schematic illustrations of a variety of DBD systems.

[0029] FIG. 2 is a schematic illustration of a reactive gas treatment system for continuous treatment of a product or a surface with a reactive gas.

[0030] FIG. 3 is a schematic illustration of a reactive gas treatment system for batch treatment of a product or a surface with a reactive gas.

[0031] FIG. 4 is a schematic illustration of a reactive gas treatment system for treatment of equipment and/or surfaces with an enclosed space

DETAILED DESCRIPTION

[0032] The present invention makes use of reactive gas produced by HVCP. The reactive gas is able to sterilize or pasteurize surfaces even when transported a significant distance from the DBD system where the plasma is produced, for example 3 to 30 meters (or 10 to 100 feet). Furthermore, the reactive gas is able to break down some organic and biological materials, such as mycotoxins. This is quite surprising, because unlike HVCP produced within a package, there is no direct exposure of the product to the HVCP, the contact time of the reactive gas with the product is limited, for example for 1 second, 1 minute, 30 minutes, or one hour. Furthermore, because the reactive gas is transported away from the DBD system where the HVCP is produced, it is diluted by both diffusion into the surrounding gas, and mixed with the surrounding gas and/or the working gas. Since the reactive gas is transported away from the DBD system, much larger volumes of product may be exposed to the reactive gas, in batch processes or continuous processes. In addition, large scale disinfection, such as disinfection of a surgical suite, may also be carried out.

[0033] FIGS. 1A, 1B, 1C, 1D, 1E and 1F are schematic illustrations of a variety of DBD systems which may be used to produce HVCP which produces a reactive gas. A DBD system includes a high voltage source, 10, having a ground which generates an alternating current, a first electrode, 20, a second electrode, 30, and an intervening dielectric, 40. One or more additional intervening dielectrics, 60, may also be present between the first and second electrode. In some configurations the dielectric may surround the first and/or second electrode. In some configurations, the charge accumulation on the electrodes, used in conjunction with the voltage waveform, may be used to estimate the power consumption of the DBD system, and may be measured by determining the voltage developed across a conventional capacitor or other sensor, 70. Preferably, a plenum, 50, is present, which defines a space between the electrodes where the HVCP and the reactive gas are produced, as shown in FIGS. 1A, 1B, 1C and 1F. However, the HVCP and reactive gas may also be produced in the vicinity of the dielectrics even when a clear plenum is not present in the DBD system, such as illustrated in FIGS. 1D and 1E. In some configurations, multiple electrodes, such as 3 to 10 electrode, 4 to 8 electrodes, or 5 to 7 electrodes, with one or more intervening dielectrics between each pair of adjacent electrode, and optionally forming multiple plenums, may be used, such as that illustrated in FIG. 1F (where a frame, 80, may be used to hold each electrode-dielectric assembly (such as 40, 20, and 40) to define each plenum (50)); such an arrangement allows for the production of a greater amount of HVCP and therefore production of reactive gas, while maintaining the appropriate distance between electrodes and keeping the system compact. The configuration of the DBD system results in limiting of the current of any filamentary discharge that is formed between the electrodes so as to prevent the formation of a high current arc. In a preferred arrangement, a first electrode is fully enclosed in a dielectric, and a second electrode is grounded.

[0034] The electrodes may be formed from any conductive material, such as a metal. The dielectrics may be formed from any insulating material (dielectric material), such as ceramics, glass, organic materials, or plastics, including multiple layers of various compositions. The thickness of the dielectric, or different layers of dielectric, should be selected to limit the current of any filamentary discharge that may form between the electrodes. Selection of materials for the dielectric layers may have an effect on the reactive gas composition.

[0035] The distance between adjacent electrodes when the electrodes are parallel, or the shortest distance between adjacent electrodes when the electrode are not parallel, is preferably at most 30 cm (or 12 inches), and preferably is at least 0.5 cm (or 0.25 inches), such as 1 to 10 cm, or 2.5 to 6 cm (or 1 to 2 inches), including 2, 3, 4, 5, 6, 7, 8 and 9 cm. The high voltage source produces a voltage of at most 500 kV, more preferably 30 kV to 150 kV, including 40, 50, 60, 70, 80, 90, 95, 100, 110, 120, 130 and 140 kV; having a frequency of at most 1000 Hz, more preferably 10 to 100 Hz, such as 50 to 60 Hz. Time variant (that is, pulsed) DC power may also be used. Although the frequency is chosen primarily for convenience (for example, 50 or 60 Hz AC power is available from the municipal power grid), voltage is selected to ensure the production of HVCP.

[0036] Different selection of working gases and working gas mixtures will affect the species present in the reactive gas produced by the HVCP. Examples of gases which may be used to prepare the HVCP include oxygen (O.sub.2); nitrogen (N.sub.2); water vapor (H.sub.2O); inert and noble gases such as helium (He), neon (Ne), argon (Ar), krypton (Kr), xenon (Xe) and sulfur hexafluoride (SF.sub.6); hydrogen (H.sub.2); carbon dioxide (CO.sub.2) and carbon monoxide (CO); halogens and pseudohalogens such as fluorine (F.sub.2), chlorine (Cl.sub.2), bromine (Br.sub.2), and cyanogen ((CN).sub.2); acidic gases such as hydrogen sulfide (H.sub.2S), hydrogen fluoride (HF), hydrogen chloride (HCl), and carbonyl sulfide (COS); ammonia (NH.sub.3); hydrazine (H.sub.4N.sub.2); nitrogen trifluoride (NF.sub.3); chlorine dioxide (ClO.sub.2); hydrocarbons such as methane (CH.sub.4), ethane (C.sub.2H.sub.6) and acetylene (H.sub.2C.sub.2); alcohols such as methanol (CH.sub.3OH) and ethanol (C.sub.2H.sub.5OH); and mixtures thereof. Preferred gases include air and MA65 (a mixture of 65% O.sub.2, 30% CO.sub.2, and 5% N.sub.2). Increasing the amount of water vapor in the gas may be used to reduce ozone present in the reactive gas. Increasing the amount of noble gas, such as helium, may be used to reduce the voltage needed to produce the HVCP. The pressure of the gas use to prepare the HVCP is conveniently selected as ambient or atmospheric pressure, but other pressures may be used, such as 10 to 50000 Torr, more preferably 100 to 1000 Torr, such as 760 Torr (atmospheric pressure).

[0037] The reactive gas contains a variety of reactive and excited species, and the reactive gas always contains at least one (and typically more than one) reactive and/or excited species which is not present in the working gas. When the working gas contains oxygen (for example, O.sub.2, CO.sub.2, and/or H.sub.2O) ozone may form; however, the properties and reactions of the reactive gas are not explained by the presence of ozone alone, and the reactive gas always contains other reactive and excited species in addition to any ozone (which may, or may not, be present in the reactive gas). In addition to ozone, other reactive and excited species which may be present in reactive gas include: singlet oxygen (.sup.1O.sub.2) and other excited molecular species (both vibrationally excited molecules and electronically excited atoms and/or molecules, such as O.sub.2, H.sub.2, N.sub.2, CO, CO.sub.2, H.sub.2O, He, Ne, Ar, Kr and Xe), hydroxyl radical (HO*), nitrogen oxides (such as N.sub.2O, NO, NO.sub.2, NO.sub.3, N.sub.2O.sub.3, N.sub.2O.sub.4 and N.sub.2O.sub.5), hydrogen peroxide (H.sub.2O.sub.2), hydroperoxyl (HO.sub.2), HNOx species (such as HNO.sub.4, HNO.sub.3 and HNO), atomic radicals (such a O, F, Cl, N and H), and molecular radicals (such as hydrocarbon radicals, which may also contain one or more of oxygen, nitrogen, fluorine and chlorine). Preferably, the reactive gas has at least one additional reactive and/or excited species in addition to ozone and NO.sub.2 (or N.sub.2O.sub.4) (which may, or may not, be present). Unlike HVCP, reactive gas is not a plasma and does not contain free electrons. Preferably, the reactive gas contains at least 2 different reactive and/or excited species listed above, more preferably at least 3 different reactive and/or excited species listed above, even more preferably at least 4 different reactive and/or excited species listed above, and most preferably at least 5 different reactive and/or excited species listed above, including 2-10 or 3-8 or 4-6 different reactive and/or excited species listed above.

[0038] It is also possible to capture and store the reactive gas in a container for later use. Preferably, the stored reactive gas is used to treat a product or surface within 24 hours after it is produced, more preferably within 12 hours, most preferably within 6, even more preferably with 3 hours.

[0039] The reactive gas may also be captured and stored by cooling to extremely low temperatures, for example using liquid nitrogen as a coolant, or using liquid helium as a coolant. When captured and stored at such low temperatures, the reactive gas may be stored for extended periods of time, for example 1 day to 6 weeks, and possibly longer. Containers, such a glass or metal containers used to store other liquefied or solidified gases, may be used.

[0040] A reactive gas treatment system includes either a DBD system or stored reactive gas, and a treatment chamber. The reactive gas treatment system also includes a device, mechanism, or a configuration for moving the reactive gas away from the DBD system (which produces a HVCP, which in turn produces the reactive gas) or from a container having stored reactive gas, and into or throughout the treatment chamber; this may be a fluid connection between the DBD system and the treatment chamber. Preferably, the treatment chamber is not sealed; such an unsealed chamber would include a treatment chamber with a gas outlet. Preferably, the treatment chamber has a volume of at least 28 liters (or 1 cubic foot), more preferably a volume of at least 1 cubic meter, and even more preferably at least 8 cubic meters. Examples of treatment chambers include rooms, bins, grain dryers, silos, tanks and shipping containers.

[0041] The reactive gas system may be used to carry out a method of treating a product and/or a surface, by supplying the reactive gas (either from stored reactive gas, or by generating a HVCP using a DBD system), and distributing the reactive gas into or throughout the treatment chamber. Examples of a device, mechanism, or configuration for moving the reactive gas includes convection, a gas pathway or gas line, a fan, and supplying flowing or pressurized working gas to the DBD system. Preferably, the product or surface treated by the reactive gas is not heated (that is, its temperature is not increased) by the method of treatment by more than 40 C., more preferably by not more than 20 C., even more preferably by not more than 10 C., and most preferably by not more than 5 C., such as no heating of the product or surface. Treatment with the reactive gas is a non-thermal processing method. Preferably, products or surfaces are not exposed to radiation (such as UV light) produced by a HVCP during the method. Optionally, air, a working gas, or another gas (such as a noble gas or nitrogen) may be used to flush the reactive gas out of the treatment chamber, or the treatment chamber may be evacuated. The method may be optionally repeated 1, 2, 3 or more times, to provide multiple treatments to products or surfaces. Optionally, product may be sealed into a container and/or refrigerated after treatment with a reactive gas. Preferably, the product to be treated is not enclosed in a sealed or substantially sealed contain, such as a container have a volume of at most 10 gallons, or at most 6 gallons, during treatment. Preferably, the HVCP is not produced inside a sealed container, such as a container have a volume of at most 10 gallons, or at most 6 gallons.

[0042] The reactive gas produced by the HVCP is transported away from the site of production of the HVCP (to avoid direct exposure of the product or surface to the HVCP), by diffusion or gas transfer. Preferably, the distance between the plasma and the product or surface to be treated is at least a distance of 5 cm, such as at least 10 cm, at least 50 cm, and at least 1 meter (or 3.28 feet), more preferably at least 3 meters, for example 3 to 300 meters, including 5, 10, 20, 30, 40 and 50 meters. In most configurations, the reactive gas is allowed to flow while it is in contact with a product or surface to be treated, although it is also possible to produce the reactive gas and transfer it to a site to treat the product or surface, and confine the gas to the treatment location for a period of time. Examples of flow rates for transferring the reactive gas to a location for contact with a product or surface include 10 to 3000 meters/minute, 30 to 2500 meters per minute, and 1000 to 2000 meters/minute, such as 50, 100, 200, 300, 400, 500, 750, and 1500 meters/minute. The reactive gas is allowed to contact the product or surface for at least 1 second, for example at least 2 seconds, at least 10 seconds, at least 30 seconds, at least 1 minute, at least 10 minutes, at least 30 minutes, at least 35 minutes, at least 1 hour, at least 6 hours, or at least 12 hours. Examples of contact times include 1 second to 12 hours, 10 seconds to 1 hour, 1 minute to 35 minutes, including 5 seconds, 15 seconds, 2 minutes, 5 minutes, 20 minutes, 35 minutes, 40 minutes, 2 hours, 3 hours, 4 hours and 5 hours.

[0043] FIG. 2 is a schematic illustration of a reactive gas treatment system, 200, for continuous treatment of a product or a surface with a reactive gas. The system includes a DBD system, 206, for generating a HVCP to produce a reactive gas, 210. The reactive gas flows along a gas pathway, 208, into a treatment chamber, 216, and then out a gas outlet, 222. Product, 214, to be treated or which has a surface to be treated, may be stored in a hopper, 212, as it is fed into the treatment chamber, and onto a conveyor, 218, which moves the product through the treatment chamber and into a receiving bin, 220, for hold the product after it has been contacted with the reactive gas. Also illustrated is a gas source, 202, such as a gas tank, which provides a working gas from which the HVCP is formed, and a gas line, 204, which supplied the DBD system with the working gas. The reactive gas may be diluted with additional working gas as it flows through the system. The transport of the reactive gas from the DBD system to the treatment chamber is by way of a pressure differential between the DBD system (at higher pressure from introduction of the working gas) and the treatment chamber (at lower pressure due to the gas outlet). Optionally, the gas outlet may be connected back to the DBD system by a second gas line, allowing for recycling of the working gas and any remaining reactive gas. Optionally, the DBD system may be located inside the treatment chamber, avoiding the need for a gas pathway. In a variation, the working gas may be air, and the transport of the reactive gas may be caused by a fan located in the gas pathway (blowing the reactive gas into the treatment chamber) or at the back of the DBD system (blowing air through the DBD system). Optionally, the conveyor may transport the product on a screen to ensure that the reactive gas comes into contact on all surfaces of the product. Furthermore, product may be moved through the treatment chamber on a plurality of conveyors, where the product is shifted around as it moves from a first conveyor to a second conveyor, ensuring that the reactive gas comes into contact with all surfaces of the product. In another variation, the DBD system may be eliminated, by using a stored reactive gas as the gas source and transporting the reactive gas directly to the treatment chamber. A variety of different conveyors may be used, such as a permeable belt conveyor, a screw, a tunnel dryer, a grain dryer or a cylindrical dryer.

[0044] FIG. 3 is a schematic illustration of a reactive gas treatment system, 300, for batch treatment of a product or a surface with a reactive gas. The system includes a DBD system, 306, for generating a HVCP to produce a reactive gas. The reactive gas flows along gas pathways, 308 and 312, into a treatment chamber, 302, and then out through a gas pathway, 316, through an optional product recovery trap, 318, along a gas pathway, 320, and out through a gas outlet, 324. Some or all of the reactive gas and working gas may be recycled back to the DBD system through an optional gas pathway, 304. The reactive gas and working gas is propelled through the system by fans, 310 and 322. Product, 314, to be treated or which has a surface to be treated, is present in the treatment chamber; as illustrated the reactive gas is fed in through the bottom of the treatment chamber to create a fluidized bed from of the reactive gas and the product to ensure treatment of all surfaces of the product. The product recovery trap may be used to capture any product which exits the treatment chamber and into the gas pathway, and return it back to the treatment chamber. The treatment chamber may be a silo in the system illustrated; other treatment chambers include a fluid bed, a mechanical fluid bed, and a bin. The reactive gas may be diluted with addition working gas as it flows through the system. As illustrated, the working gas may be air, but optionally the gas pathway, 304, may be connected to a gas source for supplying a working gas to the DBD system. In another variation, the DBD system may be eliminated and replaced with stored reactive gas.

[0045] Any product or surface may be treated with the reactive gas, to sterilize (medical sterilization or canning sterilization) or pasteurize (Salmonella pasteurized, Listeria pasteurized or E. coli pasteurized) the product or its surface, and/or removed contaminates, such as toxins. Examples of products includes fresh foods (such as fruits, vegetables, grains, beans, seeds, meat, dairy products, eggs, and spices or seasonings), seafood (fish and shell fish, and their parts), prepared foods, frozen foods, processed foods prior to packaging (water, beverages, baby food, liquid eggs, fruit juice, flour, oil, nutritional product, vitamins, nutraceuticals and baked foods), packaged products (for treatment of the exterior of the packages), animal feed, cans, bottles, plastic containers, food containers, cookware and utensils; pills, capsules, unit dosage forms and powders; medical devices and medical equipment, both before use and after use; laboratory glass and plastic ware; ceramic products; metal products; and leather and wood products.

[0046] If a sufficient reduction in viable microorganisms (or microorganism spores) is not accomplished by treatment with the reactive gas, successive treatments may be conducted until the desired reduction is achieved, for example sufficient to achieve medical sterilization or canning sterilization. For example, 1 to 10 treatments may be carried out, or 2 to 9 treatments, including 3, 4, 5, 6, 7 or 8 treatments may be carried out. Similarly, the time of treatment may also be extended. Preferably, treatment with reactive gas is repeated until medical sterilization or canning sterilization is achieved, or Salmonella pasteurization, Listeria pasteurization or E. coli pasteurization is achieved.

[0047] As with sterilization or pasteurization, if a sufficient reduction in toxin (such as mycotoxin or aflatoxin) is not accomplished by treatment with the reactive gas, successive treatments may be conducted until the desired reduction is achieved. For example, the treatment may be repeated until a reduction is achieved of at least a 50% reduction, at least a 90% reduction, at least a 110.sup.1 reduction, at least a 110.sup.2 reduction, or even at least a 110.sup.3 reduction.

[0048] Surfaces of products, rooms and container may be treated with reactive gas, to deodorize, remove pests and insects, remove or kill mold, sterilize, pasteurize, bleaching, and destroy toxins such as biological toxins and pesticides. The reactive gas may also be used to treat waste water, exhaust gases (such as automobile exhaust), chemically modify oils, and denature enzymes.

[0049] Fruits (such as fruit parts and dried fruit), and seeds (for example seed parts; grains such as wheat, rice and corn; legumes such as peas, beans, lentils, soybeans and peanuts; and nuts such as cashews, macadamia nuts, hazelnuts, chestnuts, acorns, almonds, pecans, pistachios, walnuts and Brazil nuts), in particular those contaminated with mycotoxins, such as aflatoxins, are preferred products because the reactive gas is able to destroy such toxins, making such products that were previously unsuitable for human or animal consumption, usable for such purposes. Examples of toxins which may be eliminated or reduced with contact with reactive gas include: aflatoxin (such as aflatoxin B1, B2, G1 and G2), deoxynivalenol (such as 15-acetyl deoxynivalenol and 3-acetyl deoxynivalenol), ochratoxin A, T2 toxin, HT-2 toxin, zearalenone and fumonisin (such as fumonisin B1, B2 and B3). The table below indicates the amount of various mycotoxins above which a product is not suitable for use as human food or animal feed, both in the US and Europe (EU). Reactive gas treatment, including repeated reactive gas treatment may be use to remove sufficient mycotoxins to transform a product which is not suitable for use as human food or animal feed into a product which is suitable for use as human food or animal feed.

TABLE-US-00001 TABLE 1 Recommendations and regulations for safe limits on mycotoxin concentrations in grain in the United States and European Union, as of 2008. Grain for human food Grain for animal feed Mycotoxin USA.sup.a EU.sup.b USA.sup.a EU.sup.b Aflatoxins 20 ppb 2-4 ppb.sup.c 20-300 ppb.sup.d 10-50 ppb.sup.d Deoxynivalenol 1000 ppb 750 ppb 5,000-10,000 1,750 ppb.sup.d ppb Fumonisins 200-4,000 ppb.sup.c 1,000 5,000- 4,000 ppb 100,000 ppb.sup.d ppb Zearalenone No guidance 75-100 1,000- 100-350 levels; case- ppb.sup.c 200,000 ppb.sup.d ppb.sup.d bycase basis .sup.aMunkvold, 2003a .sup.bCommission Regulation (EC) No 1126/2007 .sup.cVaries among specific food items .sup.dVaries among livestock species

[0050] FIG. 4 is a schematic illustration of a reactive gas treatment system for treatment of equipment and/or surfaces with an enclosed space, such as a room, a shipping container, a trailer or a refrigerated truck. Within the treatment chamber, 400, which here is the enclosed space, is a DBD system, 406, for generating a HVCP to produce a reactive gas, 408. A fan, 410, is used to distribute the reactive gas throughout the enclosed space. Also illustrated are product or surfaces to be treated, which includes the walls or interior surfaces of the enclosed space, optional equipment, 414, such a medical equipment (for example, surgical instruments, masks, assisted breathing equipment, and vital signs monitors), and/or optional surfaces, 412, such as a surgical table, to be treated with the reactive gas. Optionally, supports, 402, could be used to mount the DBD system to the top or the sides of the enclosed space, or the DBD system could be place on the floor of the enclosed space. Optionally, a working gas supply could be supplied by a gas line, 404, connected to a gas supply (not illustrated). Alternatively, the enclosed space could be filled with a working gas. In another configuration, the DBD system could be replaced with stored reactive gas.

EXAMPLES

[0051] The following examples are test systems to show the effects and properties of reactive gas, where a HVCP was used to produce the reactive gas. In a typical system, the scale would be increased to achieve treatment of commercially significant amounts of product. All HVCP was produced using power at 60 Hz.

Example 1: Treating Whole Corn to Reduce Microbial Load Simulating Short Duration Reactive Gas Exposure

[0052] 100 g of whole corn was place in an ArtBin Polypropylene (PP) Container (model 9100AB)size 37.0 cm35.5 cm5.2 cm (LWH). The ArtBin was place inside a second bag composed of Cryovac B2630 high barrier filmsize 40.0 cm47.0 cm (LW). Each bag was flushed for 3 minutes (37 L/min.) with MA65 (65% O.sub.2, 30% CO.sub.2, 5% N.sub.2) as fill gas and then sealed. The bag was then placed within a DBD system, between two-4 electrode sets (each electrode: aluminum, 15.24 cm diameter, 8 electrodes total4 top, 4 bottom) to produce a HVCP within the bag, but not in contact with the whole corn in the ArtBin. Treatment times were 5 minutes and 15 minutes for the whole corn samples with 280-290 watts power consumption. The height (gap) was 5.2 cm between the electrodes. The HVCP were formed at 95 kV with an amperage of 1.0-1.5 mA. The dielectric barriers were used to regulate plasma field characteristics inside the bags: (1) cutting boards (IKEA brand, 37 cm29 cm2 cm); (2) plexiglass barrier positioned on top electrode set; and (3) tote lids (Bella brand) from 114 L and/or 151 L totes (two above and one below each bag) for additional surface area extension of barrier capacity. These dielectric barriers allowed for optimal reactive gas generation from the HVCP.

[0053] Ozone and nitrogen oxides were measured by means of Drager Short-Term Detector tubes (Draeger Safety AG & Co. KGaA, Luebeck, Germany). Immediately after the treatment was complete, the bags were opened and the samples were flushed with fresh gas to remove any remaining reactive gas with the exception of one sample that was treated for 5 minutes and the reactive gas was allowed to remain in the sealed bag for 24 hours before opening.

[0054] Total aerobic bacteria colony forming units (CFU/g) were determined by standard spread plate methodology using tryptic soy agar for aerobic bacteria (TSA, Difco brand, Becton, Dickinson and Company (BD), Sparks, Md.). Standard TSA plates for aerobic recoveries were incubated at 37 C. for 24 hours. After 24 hours post reactive gas treatment and storage at room temperature (22 C.), microbial populations were recovered from respective food product(s) using a sterile rinse (0.1% peptone) by agitation for 1 minute in sterile filter stomacher bags to remove microorganisms from product surfaces. Rinse by agitation (hand shaking and vortexing) allowed for external recoveries only, without potential for additional bactericidal interference that may be introduced from internal flesh as a result of stomaching. Recoveries from diluents were obtained by performing serial dilutions and plate enumeration. Microbial colonies were enumerated after the plates were incubated at 37 C. for 24 hours. All microbiological methods were performed according to the U.S. Food and Drug Administration, Bacteriological Analytical Manual (BAM: Bacteriological Analytical Manual, 8th Edition, Final Revision: Jan. 25, 2001). Samples of the whole corn were collected from the same whole corn sample by subdividing the sample and analyzing the samples before and after treatment to obtain the differential reduction in microbial load on the corn.

[0055] The table below summarizes the results from this experiment. Temp. in the table refers to the temperature of the electrodes. Additional reduction, using successive treatments, could be used to achieve as great a reduction as desired.

TABLE-US-00002 TABLE 1 HVCP Process Parameters: 95 kV multi-electrode set up, MA65 gas type, 100 g sample quantity whole corn kernels Time Storage Temp. Log 10 Ozone NOx (min) (h) Experiment ( C.) reduction/g (ppm) (ppm) 5 0 1 32 0.35 15000 1000 2 33 0.35 15000 2000 Average 0.35 0.0 15000 0.0 1500 707 5 24 1 35 1.12 15000 1000 2 34 1.66 17500 1500 3 38 1.43 8000? 1600 4 38 1.36 15000 2000 Average 1.69 0.26 15833 1443 1525 411.3 15 0 1 48 3.05 15000 1000 2 35 1.56 10000 2000 3 38 1.75 8000 1200 Average 2.25 0.34 11000 3535 1333 707

Example 2: Treating Whole Wheat to Reduce Microbial Load Simulating Short Reactive Gas Exposure

[0056] 100 g of whole wheat was instead of whole corn, and the experiments and measurement carried out in Example 1 were repeated. The table below summarizes the results from this experiment. Temp. in the table refers to the temperature of the electrodes. Additional reduction, using successive treatments, could be used to achieve as great a reduction as desired.

TABLE-US-00003 TABLE 2 HVCP Process Parameters: 95 kV multi-electrode set up, MA65 gas type, 100 g sample of whole wheat kernels Time Storage Temp. Log10 Ozone NOx (min) (h) Experiment ( C.) reduction/g (ppm) (ppm) 5 0 1 38 0 9250 4000 2 41 0 17500 6000 Average 0 13375 5833 5000 1414 5 24 1 33 0.79 7500 1000 2 37 1.23 10000 1500 Average 1.01 0.31 8750 1767 1250 353 15 0 1 48 0.46 7000 500 2 53 0.62 5500 800 Average 0.54 0.11 6250 1060 650 212

Example 3: Treatment of a Known Reference Sample Containing Mycotoxins to Show a Reduction

[0057] 50 grams of a naturally contaminated multi-toxin corn product supplied by Trilogy Analytical Laboratory, Washington, Mo. (Trilogy Reference Material, Product #: TR-MT500, Batch#: MTC-9999E) with known concentrations of mycotoxins was place in an ArtBin Polypropylene (PP) Container (model 9100AB)size 37.0 cm35.5 cm5.2 cm (LWH). The ArtBin was place inside a second bag composed of Cryovac B2630 high barrier filmsize 40.0 cm47.0 cm (LW). Each bag was flushed for 3 minutes (37 L/min) with either Air (22% O.sub.2, 78% N.sub.2) or MA65 (65% O.sub.2, 30% CO.sub.2, 5% N.sub.2) as fill gas and then sealed. Humidification of the gas used in some of the experiments was performed using a bubble (resulting in about 60% humidity). The bag was then placed within a DBD system, between two-4 electrode sets (each electrode: aluminum, 15.24 cm diameter, 8 electrodes total4 top, 4 bottom) to produce a HVCP within the bag, but not in contact with the product in the ArtBin. The HVCP was formed at 100 kV with an amperage of 0.6-1.8 mA across all samples. The dielectric barriers were used to regulate plasma field characteristics inside the bags: (1) cutting boards (IKEA brand, 37 cm29 cm2 cm); (2) plexiglass barrier positioned on top electrode set; and (3) tote lids (Bella brand) from 114 L and/or 151 L totes (two above and one below each bag) for additional surface area extension of barrier capacity. All product samples were treated for treatment times of 30 min and then stored for 24 hours post treatment under room temperature (22 C.) conditions. After 24 hours storage, all test samples and controls were sent to Trilogy Analytical Laboratory, Washington, Mo. for a complete mycotoxin panel (#6).

[0058] The following two tables show the result from these experiments. In the table. ND means not detected. In Table 3, the total toxin in the reference was 40.67 ppm, while after treatment the total was only 13.00 ppm, resulting in a total reduction of 68%. In Table 4, the total toxin in the reference was 45.97 ppm, while after treatment the total was only 23.75 ppm, resulting in a total reduction of 48%. Additional reduction, using successive treatments, could be used to achieve as great a reduction as desired.

TABLE-US-00004 TABLE 3 Mycotoxin reduction results using MA65 working gas and 100 kV for 30 minutes Dry MA65 Humidified MA65 % Toxin Reference A-1 A-2 A-3 A-4 Reduction Aflatoxin B1 18.8 ppb 7.3 ppb 7.4 ppb 8.2 ppb 7.4 ppb 61% Aflatoxin B2 0.9 ppb ND ND ND ND 100% Aflatoxin G1 2.4 ppb ND ND ND ND 100% Aflatoxin G2 ND ND ND ND ND Deoxynivalenol 2.6 ppm 1.5 ppm 1.5 ppm 1.6 ppm 1.4 ppm 42% 15-Acetyl 0.2 ppm 0.1 ppm 0.2 ppm ND Deoxynivalenol 3-Acetyl ND ND ND ND Deoxynivalenol Ochratoxin A 4 ppb 2.7 ppb 1.9 ppb 2.7 ppb 2.4 ppb 40% T2 Toxin 263.7 ppb 228 ppb 242 ppb 245 ppb 266 ppb 0% HT-2 Toxin 523.3 ppb 521 ppb 522 ppb 530 ppb 525 ppb 0% Zearalenone 352.0 ppb ND ND ND ND 100% Fumonisin B1 28.1 ppm 8.3 ppm 8.1 ppm 7 ppm 7.3 ppm 72% Fumonisin B2 7.1 ppm 2 ppm 1.9 ppm 1.8 ppm 1.9 ppm 73% Fumonisin B3 1.7 ppm 0.9 ppm 1 ppm 0.8 ppm 0.8 ppm 53%

TABLE-US-00005 TABLE 4 Mycotoxin reduction results using air working gas at 100 kV for 30 minutes Dry Air % Toxin Reference TA-1 TA-2 Reduction Aflatoxin B1 18.9 ppb 14.9 ppb 16.5 ppb 16% Aflatoxin B2 ND ND ND Aflatoxin G1 ND ND ND Aflatoxin G2 ND ND ND Deoxynivalenol 2.3 ppm 2.4 ppm 2.2 ppm 0% 15-Acetyl 0.3 ppm 0.2 ppm 0.2 ppm Deoxynivalenol 3-Acetyl ND ND ND Deoxynivalenol Ochratoxin A 3.4 ppb 4 ppb 3.6 ppb 12% T2 Toxin 353.1 ppb 336.9 ppb 313.4 ppb 8% HT-2 Toxin 561.4 ppb 505.7 ppb 512.4 ppb 10% Zearalenone 228.6 ppb 139.7 ppb 177.6 ppb 34% Fumonisin B1 28.6 ppm 14.7 ppm 14.1 ppm 50% Fumonisin B2 10.8 ppm 4.9 ppm 4.6 ppm 56% Fumonisin B3 3 ppm 1.5 ppm 1.2 ppm 55%

Example 4: Generation and Transport of Reactive Gas

[0059] A diameter polypropylene tube with a inner diameter was fitted with two, 20 gauge insulated wires, 180 degrees apart from each other. The wires were five feet in overall length. One foot of each wire were attached to the polypropylene tube using a polyvinylchloride shrink tubing. The apparatus was placed in a stand with two vertical supports to suspend it off the ground. The tubing was connected to a compressed gas tank which had a rotometer to measure the flow of gas that was being passed through the tube. A valve and sampling valve were installed on the discharge of this DBD system to measure the amount of ozone that was being generated as a surrogate for other reactive and excited species that were being generated in addition to ozone. The amount of ozone generated was measured by means of Draeger@ Short-Term Detector tubes (Draeger Safety AG & Co. KGaA, Luebeck, Germany). The working gas used in this experiment was compressed air. Two different flow rates were used to determine if flow rate would affect the reactive and excited species generation rate. Gas flow rates were measure using the rotometer and also measured by the time required to fill a 100 ml syringe which was attached to the sampling valve. Three different measurements were taking over a 30 minute period to determine the average ozone generation rate. The conditions for generating the HVCP were the same for both experiments (30 kV) using 7 watts of power. The table below summarizes the results from this experiment.

TABLE-US-00006 TABLE 5 Generation and transport of reactive gas Gas Flow Gas Flow Rate Rate Calculated residence Rotometer Syringe time in plasma generator Ozone (ft/sec) (ft/sec) (sec) (PPM) Run 1 1.95 2.75 0.364 8 Run 2 4.0 4.59 0.218 8