POLYPHENOL COATING AND PREPARATION METHOD AND USE THEREOF

Abstract

The present disclosure relates to the technical field of coatings. In order to prepare a polyphenol coating more efficiently and gently on the surface of a material, the present disclosure provides a preparation method of a polyphenol coating, including the following steps: (1) preparing a Tanfloc aqueous solution; (2) adjusting the Tanfloc aqueous solution obtained in step (1) to be in a colloidal suspension state to obtain a modified solution; and (3) placing a carrier material in the modified solution prepared in step (2) to obtain a modified coating on a surface of the carrier material. In the present disclosure, by the preparation method of a polyphenol coating, the polyphenol coating can be efficiently prepared on a material surface under mild conditions.

Claims

1. A preparation method of a polyphenol coating, comprising the following steps: (1) preparing a Tanfloc aqueous solution; (2) adjusting the Tanfloc aqueous solution obtained in step (1) to be in a colloidal suspension state to obtain a modified solution; and (3) placing a carrier material in the modified solution prepared in step (2) to obtain a modified coating on a surface of the carrier material.

2. The preparation method of a polyphenol coating according to claim 1, wherein in step (2), the Tanfloc aqueous solution is adjusted with an alkaline solution, such that the modified solution is at a pH value of 5.5 to 8.5.

3. The preparation method of a polyphenol coating according to claim 1, wherein in step (2), the Tanfloc aqueous solution is treated with a salt solution, such that the Tanfloc aqueous solution forms a colloid.

4. The preparation method of a polyphenol coating according to claim 1, wherein in step (3), the carrier material is immersed in the modified solution for greater than or equal to 1 min.

5. The preparation method of a polyphenol coating according to claim 1, wherein in step (3), when the carrier material is placed in the modified solution, the modified solution is in an ultrasonic or vibrating environment.

6. The preparation method of a polyphenol coating according to claim 1, wherein in step (1), the Tanfloc aqueous solution has 0.05 mg/ml to 20 mg/ml of Tanfloc.

7. A polyphenol coating prepared by the preparation method of a polyphenol coating according to claim 1.

8. The polyphenol coating according to claim 7, wherein in step (2), the Tanfloc aqueous solution is adjusted with an alkaline solution, such that the modified solution is at a pH value of 5.5 to 8.5.

9. The polyphenol coating according to claim 7, wherein in step (2), the Tanfloc aqueous solution is treated with a salt solution, such that the Tanfloc aqueous solution forms a colloid.

10. The polyphenol coating according to claim 7, wherein in step (3), the carrier material is immersed in the modified solution for greater than or equal to 1 min.

11. The polyphenol coating according to claim 7, wherein in step (3), when the carrier material is placed in the modified solution, the modified solution is in an ultrasonic or vibrating environment.

12. The polyphenol coating according to claim 7, wherein in step (1), the Tanfloc aqueous solution has 0.05 mg/ml to 20 mg/ml of Tanfloc.

13. A method for surface modification of a medical material using the preparation method of a polyphenol coating according to claim 1.

14. The method according to claim 13, wherein in step (2), the Tanfloc aqueous solution is adjusted with an alkaline solution, such that the modified solution is at a pH value of 5.5 to 8.5.

15. The method according to claim 13, wherein in step (2), the Tanfloc aqueous solution is treated with a salt solution, such that the Tanfloc aqueous solution forms a colloid.

16. The method according to claim 13, wherein in step (3), the carrier material is immersed in the modified solution for greater than or equal to 1 min.

17. The method according to claim 13, wherein in step (3), when the carrier material is placed in the modified solution, the modified solution is in an ultrasonic or vibrating environment.

18. The method according to claim 13, wherein in step (1), the Tanfloc aqueous solution has 0.05 mg/ml to 20 mg/ml of Tanfloc.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0017] FIG. 1 shows a structural formula of Tanfloc used in Examples 1 to 6 of the present disclosure;

[0018] FIG. 2 shows an X-ray photoelectron spectroscopy (XPS) result provided by Example 1 of the present disclosure;

[0019] FIG. 3 shows a contact angle result provided by Example 1 of the present disclosure;

[0020] FIG. 4 shows a Zeta potential result provided by Example 1 of the present disclosure; and

[0021] FIG. 5A-B show an XPS result provided by Example 4 of the present disclosure.

DETAILED DESCRIPTION OF THE EMBODIMENTS

[0022] FIG. 1 shows a structural formula of Tanfloc used in Examples 1 to 6.

Example 1

[0023] In this example, a preparation method of a polyphenol coating included the following steps:

[0024] (1) Cleaning of a carrier material: a polished carrier material was placed into an ultrasonic cleaner, and washed in acetone and absolute alcohol separately for 5 min to 10 min by an ultrasonic wave at 15 kHz to 30 kHz, and then dried.

[0025] (2) A 1 mg/ml Tanfloc solution was prepared, adjusted to a pH value of 7.0 with 1 M sodium hydroxide, and the solution was immersed into a colloid to be in a colloidal suspension state to form a modified solution; and a cleaned carrier material was immersed in the modified solution for 10 min under an ultrasonic environment.

[0026] (3) The un-adsorbed Tanfloc was removed by washing with RO water to obtain a modified coating.

[0027] A coating state on the surface of the carrier material was detected by XPS, and the results were shown in FIG. 2. It was seen from FIG. 2 that the XPS results on a left showed that a Ti/C peak was inverted, and a new N element peak appeared, proving that Tanfloc in a colloidal suspension state could form a coating on the surface of medical implants; and SEM results on a right side showed that the formed coating had a thickness close to 10 nm. It was confirmed that the preparation method of the present disclosure could quickly form a coating on the surface of a medical implant under mild conditions.

[0028] FIG. 3 showed changes of a contact angle before and after the polyphenol coating was deposited on the carrier material. It was seen from the results that the contact angle on the surface of the polyphenol coating-coated carrier material was significantly reduced. FIG. 4 showed surface Zeta potential changes before and after the polyphenol coating was deposited on the carrier material. It was seen from the results that the Zeta potential of the polyphenol coating surface was significantly reduced. The above results indicated that the polyphenol coating not only formed a coating on the surface of the carrier material, but also significantly changed the physical and chemical properties of the material surface.

Example 2

[0029] In this example, a preparation method of a polyphenol-copper ion coating included the following steps:

[0030] (1) Cleaning of a carrier material: a polished carrier was placed into an ultrasonic cleaner, and washed in acetone and absolute alcohol separately for 5 min to 10 min by an ultrasonic wave at 15 kHz to 30 kHz, and then dried.

[0031] (2) A 1 mg/ml Tanfloc solution was prepared, adjusted to a pH value of 7.0 with 6 M sodium hydroxide, and the solution was immersed into a colloid to be in a colloidal suspension state to form a modified solution; and a cleaned carrier material was immersed in the modified solution for 10 min under an ultrasonic environment.

[0032] (3) The un-adsorbed Tanfloc was removed by washing with RO water to obtain a modified coating.

[0033] (4) The carrier material containing the modified coating was immersed in a 1 mg/ml cupric chloride solution for 3 min.

[0034] (5) The un-adsorbed ions were removed by washing with RO water to obtain a Tanfloc-copper ion composite coating.

Example 3

[0035] In this example, a preparation method of a polyphenol coating included the following steps:

[0036] (1) Cleaning of a carrier material: a polished carrier was placed into an ultrasonic cleaner, and washed in acetone and absolute alcohol separately for 5 min to 10 min by an ultrasonic wave at 15 kHz to 30 kHz, and then dried.

[0037] (2) A 1 mg/ml Tanfloc solution was prepared, added with an equal amount of normal saline, and the solution was immersed into a colloid to be in a colloidal suspension state to form a modified solution; and a cleaned carrier material was immersed in the colloidal suspension for 10 min under an ultrasonic environment.

[0038] (3) The un-adsorbed Tanfloc was removed by washing with RO water to obtain a coating.

Example 4

[0039] In this example, a preparation method of a polyphenol coating on a contaminated implant surface included the following steps:

[0040] (1) Cleaning of a carrier material: a polished carrier was placed into an ultrasonic cleaner, and washed in acetone and absolute alcohol separately for 5 min to 10 min by an ultrasonic wave at 15 kHz to 30 kHz, and then dried.

[0041] (2) Preparation of a contaminated carrier material surface: the carrier material was immersed in a 5 ?g/ml LPS aqueous solution for 1 h, the un-adsorbed LPS was removed by rinsing with RO water, and the carrier material was dried to obtain a carrier material contaminated by LPS.

[0042] (3) A 1 mg/ml Tanfloc solution was prepared, adjusted to a pH value of 7.0 with 1 M sodium hydroxide, and the solution was immersed into a colloid to be in a colloidal suspension state to form a modified solution; and the contaminated carrier material obtained in step (2) was immersed into the modified solution under a shaker for 10 min.

[0043] (3) The un-adsorbed Tanfloc was removed by washing with RO water to obtain a polyphenol coating.

[0044] Meanwhile, XPS was conducted to detect the polyphenol coating obtained in this example and the polyphenol coating in a control group (the control group was to directly immerse the carrier material in the Tanfloc solution (non-modified solution)), and the results were shown in FIG. 5A-B. It was seen from FIG. 5A-B that unmodified Tanfloc had less deposition on the surface of medical implants contaminated by LPS (in FIG. 5A, the Ti/C peak failed to reverse); however, the Tanfloc under the modified solution could be deposited in large quantities on the surface of medical implants contaminated by LPS (in FIG. 5B, the Ti/C peak was reversed). It was proved that the preparation method of the present disclosure could form a polyphenol coating on the surface of medical implants even after being contaminated by LPS (a common substance from bacterial contamination).

Example 5

[0045] In this example, a preparation method of a polyphenol coating on an implant in vivo included the following steps:

[0046] (1) A 1 mg/ml Tanfloc solution was prepared, adjusted to a pH value of 7.0 with 1 M sodium hydroxide, and the solution was immersed into a colloid to be in a colloidal suspension state to form a modified solution;

[0047] (2) after exposing an implant in vivo during operation, a surface of the implant was rinsed for 3 min to 5 min with the modified solution in step (1), while excess liquid was removed using a suction device; and

[0048] (3) The excess liquid was removed by rinsing with normal saline.

Example 6

[0049] In this example, a preparation method of a polyphenol coating on a contaminated intraoral implant surface included the following steps:

[0050] (1) A 1 mg/ml Tanfloc solution was prepared, adjusted to a pH value of 7.0 with 1 M sodium hydroxide, and the solution was immersed into a colloid to be in a colloidal suspension state to form a modified solution;

[0051] (2) after exposing threads of the intraoral implant during operation, a surface of the intraoral implant was rinsed with the modified solution for 3 min to 5 min using an ultrasonic irrigator, while excess liquid was removed using a suction device; and

[0052] (3) The excess liquid was removed by rinsing with normal saline.

[0053] The above examples are merely preferred examples of the present disclosure and are not intended to limit the present disclosure, and various changes and modifications may be made to the present disclosure by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and scope of the present disclosure should be included within the protection scope of the present disclosure.