Reaction container and method for producing same

Abstract

The invention relates to a reaction container and a method for producing the reaction container, and provides a low-cost reaction container which is capable of consistently, quickly, and efficiently performing a process that includes a reaction such as extraction or amplification of a nucleic acid, while saving user's trouble without increasing the scale of the device. The reaction container is configured to comprise: one or two or more housing parts for reactions comprising: a narrow-mouthed piping part in which a reaction reagent or a portion thereof is housed or is housable; a wide-mouthed piping part that is communicated with the narrow-mouthed piping part and provided on an upper side of the narrow-mouthed piping part, and has an aperture that is wider than an aperture of the narrow-mouthed piping part; and a punchable film provided such that it partitions an interval between the wide-mouthed piping part and the narrow-mouthed piping part.

Claims

1. A reaction container comprising: one or two or more housing parts for reactions comprising: a narrow-mouthed piping part formed with a rotation symmetry and in which a reaction reagent or a portion thereof is housed or is housable; a wide-mouthed piping part that is communicated with the narrow-mouthed piping part and provided on an upper side of the narrow-mouthed piping part, the wide-mouthed piping part being formed with a rotation symmetry coaxially with the narrow-mouthed piping part and having an aperture that is wider than an aperture of said narrow-mouthed piping part; and a punchable film provided such that it partitions an interval between the wide-mouthed piping part and the narrow-mouthed piping part, the punchable film, when punched, being insertable into the aperture of said narrow-mouthed piping part; and a sealing lid that seals the reaction container by being mounted on the aperture of said wide-mouthed piping part of said reaction container, said sealing lid comprising: a plug portion defining an internal cavity and an exterior bottom surface having transparency, wherein the plug portion is fittable to an inside wall defined by the aperture of said wide-mouthed piping part and is able to guide light from said narrow-mouthed piping part; and a pushing portion provided to the exterior bottom surface of said plug portion so that, after the film has been punched and during the fitting of the plug portion with the wide-mouthed piping part, the pushing portion pushes said film, which has been already punched, into the aperture of the narrow-mouthed piping part, and against an inner wall of the narrow-mouthed piping part, thus enabling the plug portion to guide light from said narrow-mouthed piping part; wherein the pushing portion comprises a cylindrical wall extending vertically downward from the exterior bottom surface of the sealing lid and formed so as to circularly enclose a center portion, through which the light guided by the sealing lid passes, adjacent the exterior bottom surface, which exterior bottom surface encloses the center portion at one end of the cylindrical wall to separate the center portion from the internal cavity of the plug portion; wherein, when the plug portion is fitted to the inside wall defined by the aperture of the wide-mouthed piping part, at least a portion of the pushing portion extends downward into the narrow-mouthed piping part and an annular region is defined between an exterior surface of the cylindrical wall of the pushing portion and the inner wall of the narrow-mouthed piping part, so that no portion of the exterior surface contacts the inner wall at any location between the exterior bottom surface of the plug portion and a bottom end of the pushing portion; and wherein the cylindrical wall of the pushing portion extends vertically downward from the exterior bottom surface of the sealing lid by a vertical length that is longer than a radial length of the annular region so that, when the plug portion is fitted to the inside wall defined by the aperture of the wide-mouthed piping part, the cylindrical wall of the pushing portion extends downward to push said punched film into the aperture of the narrow-mouthed piping part and against the inner wall of the narrow-mouthed piping part.

2. A reaction container according to claim 1, in which the aperture of said narrow-mouthed piping part is provided at the center of a bottom portion of the wide-mouthed piping part.

3. A reaction container according to claim 1, wherein said narrow-mouthed piping part and said wide-mouthed piping part are integrally formed, and said film is attached to a bottom portion of said wide-mouthed piping part.

4. A reaction container according to claim 1 that has a cartridge container which further has a base plate in which two or more concave portions are arranged in a single row form, and said housing part for reactions is formed in one of the concave portions, and in the other of the concave portions excluding the concave portion in which said housing part for reactions is formed, instruments for performing processing that are moved to said housing part for reactions are housed or are housable.

5. A reaction container according to claim 4, wherein said other concave portions excluding the concave portion in which said housing part for reactions is formed, are provided with a sealing lid housing part that houses said sealing lid, a tip for punching housing part that houses a tip for punching that punches said film, and/or a dispensing tip housing part that houses a dispensing tip.

6. A reaction container according to claim 4, wherein said wide mouth piping part of said container for reaction formed in said concave portion comprises a concave portion having an aperture formed in said base plate, and said narrow-mouthed piping part is separately formed from said wide-mouthed piping part, a hole portion is piercingly provided in the center of a bottom portion of the wide-mouthed piping part, said narrow-mouthed piping part has an aperture edge portion that encloses the aperture thereof, said narrow-mouthed piping part downwardly protrudes from said hole portion of the wide-mouthed piping part such that it passes through said hole portion, said aperture edge portion is installed on the bottom portion of said wide-mouthed piping part, and said film is attached to said aperture edge portion of said narrow-mouthed piping part.

7. A reaction container according to claim 1, wherein said wide-mouthed piping part or said sealing lid are adapted to be able to be linked with a linking portion, the linking portion including ends of one or two or more light guide portions so that, when the linking portion is linked with said wide-mouthed piping part or said sealing lid, the light guide portions optically connect a light measuring device provided on the exterior of the reaction container with the interior of the housing part for reactions.

8. A reaction container according to claim 1, wherein said sealing lid has: a cavity provided in the center thereof; and a bottom surface, which has transparency, that blocks a lower end of the cavity, and movement of the sealing lid to the housing part for reactions, and the fitting and/or the linking with said linking portion, is performed by inserting a member provided on the exterior of the reaction container and/or said linking portion into said cavity.

9. A reaction container comprising: one or two or more housing parts for reactions comprising: a narrow-mouthed piping part formed with a rotation symmetry and in which a reaction reagent or a portion thereof is housed or is housable; a wide-mouthed piping part that is communicated with the narrow-mouthed piping part and provided on an upper side of the narrow-mouthed piping part, the wide-mouthed piping part being formed with a rotation symmetry coaxially with the narrow-mouthed piping part and having an aperture that is wider than an aperture of said narrow-mouthed piping part; and a punchable film provided such that it partitions an interval between the wide-mouthed piping part and the narrow-mouthed piping part, the punchable film, when punched, being insertable into the aperture of said narrow-mouthed piping part; and a sealing lid that seals the reaction container by being mounted on the aperture of said wide-mouthed piping part of said reaction container, said sealing lid comprising: a plug portion defining an internal cavity and an exterior bottom surface having transparency, wherein the plug portion is fittable to an inside wall defined by the aperture of said wide-mouthed piping part and is able to guide light from said narrow-mouthed piping part; and a pushing portion provided to the exterior bottom surface of said plug portion so that, after the film has been punched and during the fitting of the plug portion with the wide-mouthed piping part, the pushing portion pushes said film, which has been already punched, into the aperture of the narrow-mouthed piping part, and against an inner wall of the narrow-mouthed piping part, thus enabling the plug portion to guide light from said narrow-mouthed piping part; wherein said wide-mouthed piping part and said narrow-mouthed piping part are separately formed, said wide-mouthed piping part having a hole portion piercingly provided in the center of a bottom portion thereof, said narrow-mouthed piping part having an aperture edge portion provided along an outer circumference of the aperture thereof, so as to enclose the aperture, and said film being attached to said aperture edge portion; wherein, excluding for said aperture edge portion, the narrow-mouthed piping part is able to pass through said hole portion such that said aperture edge portion is installed on the bottom of said wide-mouthed piping part and said narrow-mouthed piping part downwardly protrudes from said hole portion of said wide-mouthed piping part; wherein the pushing portion comprises a cylindrical wall extending vertically downward from the exterior bottom surface of the sealing lid and formed so as to circularly enclose a center portion, through which the light guided by the sealing lid passes, adjacent the exterior bottom surface, which exterior bottom surface encloses the center portion at one end of the cylindrical wall to separate the center portion from the internal cavity of the plug portion; wherein, when the plug portion is fitted to the inside wall defined by the aperture of the wide-mouthed piping part, at least a portion of the pushing portion extends downward into the narrow-mouthed piping part and an annular region is defined between an exterior surface of the cylindrical wall of the pushing portion and the inner wall of the narrow-mouthed piping part, so that no portion of the exterior surface contacts the inner wall at any location between the exterior bottom surface of the plug portion and a bottom end of the pushing portion; and wherein the cylindrical wall of the pushing portion extends vertically downward from the exterior bottom surface of the sealing lid by a vertical length that is longer than a radial length of the annular region so that, when the plug portion is fitted to the inside wall defined by the aperture of the wide-mouthed piping part, the cylindrical wall of the pushing portion extends downward to push said punched film into the aperture of the narrow-mouthed piping part and against the inner wall of the narrow-mouthed piping part.

10. A reaction container according to claim 9, wherein said wide-mouthed piping part or said sealing lid are adapted to be able to be linked with a linking portion, the linking portion including ends of one or two or more light guide portions so that, when the linking portion is linked with said wide-mouthed piping part or said sealing lid, the light guide portions optically connect a light measuring device provided on the exterior of the reaction container with the interior of the housing part for reactions.

11. A reaction container according to claim 10, wherein said sealing lid has a cavity provided in the center thereof, wherein the exterior bottom surface, which has transparency, blocks a lower end of the cavity, and movement of the sealing lid to the housing part for reactions, and the fitting and/or the linking with said linking portion, is performed by inserting a member provided on the exterior of the reaction container and/or said linking portion into said cavity.

12. A reaction container according to claim 9 that has a cartridge container which further has a base plate in which two or more concave portions are arranged in a single row form, and said housing part for reactions is formed in one of the concave portions, and in the other of the concave portions excluding the concave portion in which said housing part for reactions is formed, instruments for performing processing that are moved to said housing part for reactions are housed or are housable.

13. A reaction container according to claim 12, wherein said other concave portions excluding the concave portion in which said housing part for reactions is formed, are provided with a sealing lid housing part that houses said sealing lid, a tip for punching housing part that houses a tip for punching that punches said film, and/or a dispensing tip housing part that houses a dispensing tip.

14. A reaction container according to claim 12, wherein said wide mouth piping part of said container for reaction formed in said concave portion comprises a concave portion having an aperture formed in said base plate.

15. A method comprising: providing a narrow-mouthed piping part of a reaction container in which a reaction reagent or a portion thereof is housed or is housable, the narrow-mouthed piping part including a first aperture and an aperture edge portion provided along an outer circumference of the first aperture, attaching a punchable film to said aperture edge portion of said narrow-mouthed piping part so that the punchable film, when punched, is insertable into the first aperture, providing a wide-mouthed piping part of the reaction container including a second aperture and a hole portion piercingly provided in a center of a bottom portion of the wide-mouthed piping part, the second aperture being wider than the first aperture, passing said narrow-mouthed piping part, excluding the aperture edge portion, through said hole portion of the wide-mouthed piping part such that said aperture edge portion is installed on the bottom portion of said wide-mouthed piping part and at least part of the narrow-mouthed piping part downwardly protrudes from the hole portion of the wide-mouthed piping part, punching the punchable film, and sealing the reaction container by mounting a sealing lid on the second aperture, the sealing lid comprising: a plug portion defining an internal cavity and an exterior bottom surface having transparency, wherein mounting the sealing lid on the second aperture fits the plug portion to an inside wall defined by the second aperture so that the sealing lid is able to guide light from the narrow-mouthed piping part via at least the exterior bottom surface, and a pushing portion extending from the exterior bottom surface of the plug portion, wherein mounting the sealing lid on the second aperture causes the pushing portion to push the punched film into the first aperture and against an inner wall of the narrow-mouthed piping part, thus enabling the plug portion to guide light from the narrow-mouthed piping part, wherein the pushing portion comprises a wall extending from the exterior bottom surface of the sealing lid and formed so as to circularly enclose a center portion, through which the light guided by the sealing lid passes, adjacent the exterior bottom surface, wherein the exterior bottom surface separates the center portion from the internal cavity of the plug portion, wherein fitting the plug portion to the inside wall defined by the second aperture extends at least part of the pushing portion downward into the narrow-mouthed piping part so that an annular region is defined between an exterior surface of the wall of the pushing portion and the inner wall of the narrow-mouthed piping part, wherein no portion of the exterior surface contacts the inner wall at any location between the exterior bottom surface of the plug portion and a bottom end of the pushing portion, and wherein the wall of the pushing portion extends from the exterior bottom surface of the sealing lid by a vertical length that is longer than a radial length of the annular region so that fitting the plug portion to the inside wall defined by the second aperture extends the pushing portion downward to push said punched film into the first aperture of the narrow-mouthed piping part and against the inner wall of the narrow-mouthed piping part.

16. A system, comprising: a nozzle head provided with a suction-discharge mechanism and two or more nozzles to which dispensing tips are detachably mountable, wherein suction and discharge of liquids is possible using the suction-discharge mechanism and the nozzles; a container group including two or more reaction containers in which a solution for reaction is housed or is housable; a nozzle transfer mechanism adapted to relatively move said nozzle head and/or said container group; a temperature controller adapted to control an interior temperature of said reaction containers; a light guide stage including two or more linking portions that are directly or indirectly linkable with said reaction containers, wherein respective first ends of two or more light guide portions are provided to the linking portions so that, when the linking portions are directly or indirectly linked with the reaction containers, the light guide portions are optically connected with the respective interiors of the linked reaction containers; a connecting end arranging body including an arranging surface that supports two or more connecting ends along a predetermined path, wherein respective second ends of said light guide portions are provided to the connecting ends; a measuring device provided adjacent said arranging surface so that, by means of optical connections with the respective connecting ends, the measuring device is able to receive light based on optical states within said reaction containers, wherein the measuring device and/or said respective connecting ends are relatively movable such that the measuring device is successively optically connected to the respective connecting ends, wherein each of the reaction containers comprises: a narrow-mouthed piping part in which a reaction reagent is housed or is housable, the narrow-mouthed piping part including a first aperture; a wide-mouthed piping part that is communicated with the narrow-mouthed piping part and provided on an upper side of the narrow-mouthed piping part, the wide-mouthed piping part including a second aperture that is wider than the first aperture of said narrow-mouthed piping part; and a punchable film provided such that it partitions an interval between the wide-mouthed piping part and the narrow-mouthed piping part, the punchable film, when punched, being insertable into the first aperture of the narrow-mouthed piping part, and wherein the linking portions are linkable with the reaction containers via sealing lids, each of the sealing lids comprising: a plug portion defining an internal cavity and an exterior bottom surface having transparency, wherein the plug portion is fittable to an inside wall defined by the second aperture and is able to guide light from the narrow-mouthed piping part, and a pushing portion extending from the exterior bottom surface of the plug portion so that, after the punchable film has been punched and during fitting of the plug portion with the wide-mouthed piping part, the pushing portion pushes the punched film into the first aperture and against an inner wall of the narrow-mouthed piping part, thus enabling the plug portion to guide light from the narrow-mouthed piping part, wherein the pushing portion comprises a wall extending from the exterior bottom surface of the sealing lid and formed so as to circularly enclose a center portion, through which the light guided by the sealing lid passes, adjacent the exterior bottom surface, wherein the exterior bottom surface separates the center portion from the internal cavity of the plug portion, wherein, when the plug portion is fitted to the inside wall defined by the second aperture, at least part of the pushing portion extends downward into the narrow-mouthed piping part and an annular region is defined between an exterior surface of the wall of the pushing portion and the inner wall of the narrow-mouthed piping part, so that no portion of the exterior surface contacts the inner wall at any location between the exterior bottom surface of the plug portion and a bottom end of the pushing portion, and wherein the wall of the pushing portion extends vertically downward from the exterior bottom surface of the sealing lid by a vertical length that is longer than a radial length of the annular region so that, when the plug portion is fitted to the inside wall defined by the second aperture of the wide-mouthed piping part, the wall of the pushing portion extends downward to push said punched film into the first aperture and against the inner wall of the narrow-mouthed piping part.

17. A reaction container system according to claim 16, wherein said container group additionally has: two or more liquid housing parts that house; a sample, a magnetic particle suspension in which magnetic particles that are able to capture a target substance of a reaction are suspended, and a solution for separating and extracting used for the separation and the extraction of said target substance; and a magnetic force part that is able to apply or remove a magnetic field to the interior of said dispensing tips mounted on said nozzles or the liquid housing parts provided to said container group, and which is able to adsorb said magnetic particles on an inner wall of said dispensing tips or said liquid housing parts.

18. A reaction container, comprising: a housing part, comprising: a narrow-mouthed piping part formed with a rotation symmetry and in which a reaction reagent or a portion thereof is housed or is housable, the narrow-mouthed piping part comprising: a frusto-conically-shaped wall, the frusto-conically-shaped wall extending angularly downward, the frusto-conically shaped wall defining a frusto-conical inside surface; a bottom wall to which the frusto-conically-shaped wall extends, the bottom wall defining a bottom inside surface that is contiguous with the frusto-conical inside surface, wherein the frusto-conical inside surface and the bottom inside surface at least partially define a first internal region in which the reaction reagent or a portion thereof is housed or is housable; an intermediate portion connected to the frusto-conically-shaped wall and from which the frusto-conically-shaped wall extends angularly downward, wherein the intermediate portion is formed with a rotation symmetry coaxially with the narrow-mouthed piping part and defines a first circular opening circumscribed by the frusto-conical inside surface at the connection between the intermediate portion and the frusto-conically-shaped wall; wherein the first circular opening has a first diameter; wherein the intermediate portion defines an annular horizontally-extending inside surface and an annular rounded inside surface extending therefrom, wherein the annular rounded inside surface also circumscribes the first circular opening of the intermediate portion; wherein the annular rounded inside surface extends from the annular horizontally-extending inside surface to the frusto-conical inside surface; and wherein the annular horizontally-extending inside surface, the annular rounded inside surface, the frusto-conical inside surface, and the bottom inside surface are contiguous; and a first cylindrical wall extending upward and opposite the downward extension of the frusto-conically-shaped wall, the first cylindrical wall being formed with a rotation symmetry coaxially with the narrow-mouthed piping part and defining a first cylindrical vertically-extending inside surface that is perpendicular to the annular horizontally-extending inside surface; wherein the first cylindrical wall defines a second circular opening, the second circular opening having a second diameter that is greater than the first diameter of the first circular opening; and wherein the first cylindrical vertically-extending surface and the annular horizontally-extending inside surface at least partially define a second internal region; a sealing lid, comprising: a plug portion that extends through the second circular opening and within the second internal region, the plug portion comprising: a second cylindrical wall, the second cylindrical wall defining a first outside surface that contacts the first cylindrical vertically-extending inside surface of the housing part, the second cylindrical wall further defining a second vertically-extending inside surface; and a circular horizontally-extending end cap connected to the lower end of the second cylindrical wall; wherein the circular horizontally-extending end cap is transparent; wherein the circular horizontally-extending end cap defines a circular inside surface; and wherein the second vertically-extending inside surface and the circular inside surface at least partially define an internal cavity; and a pushing portion connected to the plug portion, the pushing portion comprising a third cylindrical wall extending vertically downward from the circular horizontally-extending end cap of the plug portion and into the first circular opening, the third cylindrical wall being formed so as to circularly enclose a center portion, through which light guided by the sealing lid passes, adjacent the circular horizontally-extending end cap, which circular horizontally-extending end cap encloses the center portion at one end of the cylindrical wall to separate the center portion from the internal cavity of the plug portion; wherein the third cylindrical wall defines a third diameter that is less than the first diameter of the first circular opening so that the third cylindrical wall of the pushing portion is able to extend into the first circular opening of the housing part, and wherein the third cylindrical wall of the pushing portion extends downward into the narrow-mouthed piping part, and an annular region is defined between an exterior surface of the third cylindrical wall and the frusto-conical inside surface defined by the frusto-conically shaped wall of the narrow-mouthed piping part, so that no portion of the exterior surface contacts the frusto-conical inside surface at any location between the circular horizontally-extending end cap of the plug portion and a bottom end of the pushing portion; and a film positioned between the housing part and the sealing lid so that: the film extends between the annular horizontally-extending inside surface of the housing part and the circular horizontally-extending end cap of the sealing lid; an opening is defined in the film due to the extension of the third cylindrical wall into the first circular opening; and the film extends between the annular rounded inside surface of the housing part and the third cylindrical wall of the plug portion of the sealing lid; wherein the housing part, the sealing lid, and the film are configured so that the plug portion is able to guide light: from the first internal region of the narrow-mouthed piping part of the housing part, vertically along the third cylindrical wall of the pushing portion of the sealing lid, through the transparent circular horizontally-extending end cap of the plug portion of the sealing lid, and into the internal cavity of the sealing lid; and wherein the third cylindrical wall of the pushing portion extends vertically downward from the circular horizontally-extending end cap of the plug portion by a vertical length that is longer than a radial length of the annular region so that the third cylindrical wall of the pushing portion extends downward to push said film into the first circular opening of the intermediate portion and against the frusto-conical wall of the narrow-mouthed piping part.

19. The reaction container of claim 18, wherein the narrow-mouthed piping part, the intermediate portion, and the first cylindrical wall of the housing part are integrally formed.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

(1) FIG. 1 is a drawing showing a housing part for reactions according to a first embodiment of the present invention.

(2) FIG. 2 is a processing explanatory drawing with regard to the housing part for reactions according to the first embodiment of the present invention.

(3) FIG. 3 is a drawing showing a sealing lid and the housing part for reactions according to the first embodiment of the present invention mounted with the same.

(4) FIG. 4 is a drawing in which a linking portion is linked with the housing part for reactions according to the first embodiment of the present invention.

(5) FIG. 5 is a housing part for reactions according to a second embodiment of the present invention, and a processing explanatory drawing thereof.

(6) FIG. 6 is a drawing in which a linking portion is linked with the housing part for reactions according to the second embodiment of the present invention.

(7) FIG. 7 is a housing part for reactions according to a third embodiment of the present invention, and a processing explanatory drawing thereof.

(8) FIG. 8 is a drawing showing a sealing lid and a housing part for reactions according to a fourth embodiment of the present invention mounted with the same.

(9) FIG. 9 is a drawing in which a linking portion is linked with the housing part for reactions according to the fourth embodiment of the present invention.

(10) FIG. 10 is a drawing showing a cartridge container according to a fifth embodiment of the present invention.

(11) FIG. 11 is a drawing showing a cartridge container according to a sixth embodiment of the present invention.

(12) FIG. 12 is an overall perspective view showing a reaction container system according to a seventh embodiment of the present invention.

(13) FIG. 13 is a plan view showing enlarged, a container group of the reaction container system shown in FIG. 12.

(14) FIG. 14 is a plan view showing enlarged, the whole nozzle head of the reaction container system shown in FIG. 12.

(15) FIG. 15 is a front side perspective view of the nozzle head of the reaction container system shown in FIG. 14.

(16) FIG. 16 is a perspective view showing more specifically the transfer mechanism and the suction-discharge mechanism shown in FIG. 14.

(17) FIG. 17 is a perspective view showing more specifically the suction-discharge mechanism shown in FIG. 14.

(18) FIG. 18 is a perspective view of the nozzle head shown in FIG. 14 viewed from the rear side.

BEST MODE FOR CARRYING OUT THE INVENTION

(19) Next, an embodiment of the present invention is described with reference to the drawings. This embodiment is not to be interpreted as limiting the present invention unless particularly specified. Furthermore, in the embodiments, the same objects are denoted by the same reference symbols, and the descriptions are omitted.

(20) FIGS. 1(a) and 1(b) are drawings showing a housing part for reactions, which represents a reaction container, according to a first embodiment of the present invention.

(21) The housing part for reactions 1 has: a narrow-mouthed piping part 1b, whereby a solution for nucleic acid amplification or a portion thereof, which represents the reaction reagent for nucleic acid amplification, is housable; a wide-mouthed piping part 1a that communicates with the narrow-mouthed piping part 1b, is provided on the upper side of the narrow-mouthed piping part 1b, and has a wider aperture than an aperture le of the narrow-mouthed piping part lb; and a punchable film 1c formed from aluminum foil or the like, provided such that it partitions the interval between the wide-mouthed piping part 1a and the narrow-mouthed piping part 1b. By means of sealing the narrow-mouthed piping part 1b in an empty state with the film 1c, contamination of the narrow-mouthed piping part 1b can be prevented. The aperture 1e of the narrow-mouthed piping part 1b is provided at the center portion of a bottom portion 1d of the wide-mouthed piping part 1a. Furthermore, the narrow-mouthed piping part 1b and the wide-mouthed piping part 1a are integrally formed, and the film 1c is attached to the bottom portion 1d of the wide-mouthed piping part 1a.

(22) FIG. 2 is a drawing showing the action in a case where, using a tip for punching 212 mounted on a nozzle head 50 mentioned below, the film 1c of the housing part for reactions 1 shown in FIG. 2(a) is punched, as shown in FIG. 2(b). FIG. 2(c) is a drawing showing a state in which a reaction reagent solution is housed following punching of the film 1c.

(23) FIG. 3 is a drawing showing a reaction container 2 according to the first embodiment of the present invention, which comprises a lid 251 and a housing part for reactions 1 sealed by the lid 251.

(24) As shown in FIG. 3(a) and FIG. 3(b), the sealing lid 251 which has transparency, has transparency, and has a plug portion 251 a that is fittable to the aperture of the wide-mouthed piping part 1a, and a cylindrical member 251c provided on the upper side of the plug portion 251a and on which a plurality of protrusions 251b along the axial direction are arranged on the outside surface. Furthermore, the plug portion 251a is provided with a circular protrusion 251e that protrudes along the outer circumference thereof in the radial direction, and adheres to the inner wall of the wide-mouthed piping part 1a. The lower side of the plug portion 251a is provided with a pushing portion 251d that is inserted into the aperture of the narrow-mouthed piping part 1b, and pushes the punched punchable film 1c into the rim of the aperture.

(25) As shown in FIG. 4, the reaction containers 2, which comprise a housing part for reactions 1 sealed by the sealing lid 251, are further respectively housed in a plurality of indentations arranged in a single row form (front-to-rear direction in the drawing) of a block 29a of a temperature controller 29 that performs the temperature control necessary for nucleic acid amplification reactions. The block 29a is heated and cooled by means of a Peltier element 29b and a heat sink 29c. The upper side of the housing parts for reactions 1 is provided with a light guide stage 147 that is mounted such that a single measuring device moves and is able to successively optically connect with the plurality of housing parts for reactions 1. Furthermore, a plurality of linking portions 141, are provided such that they downwardly protrude from the light guide stage 147 in a single row form (front-to-rear direction in the drawing). The respective linking portions 141 are inserted into a cavity 251f (see FIG. 3) of the respective sealing lids 251, and since the light guide portions 142 that pass through the interior of the linking portions 141 have a size that approximately covers the apertures of the narrow-mouthed piping parts 1b, the light within the narrow-mouthed piping part 1b can be guided with certainty via the light guide portion 142 to the measuring device on the light guide stage 147 without loss. Consequently, by utilizing the stable light receivable time of the fluorescence, and the like, that is generated at the time amplification reactions of nucleic acids (DNA, RNA and the like) and the fragments thereof (oligonucleotides, nucleotides and the like) are performed using the real-time PCR method, and by moving a single measuring device, measurements relating to the plurality of housing parts for reactions 1 are made possible, and the overall device construction can be made compact. Reference symbol 143 is a lens, reference symbol 144 is a hot lid, reference symbol 145 is a seat heater, reference symbol 146 is a heat insulator, and reference symbol 148 is a pressing ring of the lens, and the prevention of condensation of the sealing lid, which is a sign of the nucleic acid amplification reaction, is achieved as a result of these. Furthermore, the groove 149 provided in the light guide stage 147 is one that guides the movement (front-to-rear direction in the drawing) of the measuring device such that the measuring device becomes successively connectable with the light guide portions 142 that correspond to the respective housing parts for reactions 1.

(26) FIG. 5 is a housing part for reactions 3 according to a second embodiment of the present invention, and a processing explanatory drawing thereof.

(27) As shown in FIG. 5(a), the housing part for reactions 3 according to the second embodiment has: a narrow-mouthed piping part 3b whereby a solution for nucleic acid amplification or a portion thereof 3f, which represents the reaction reagent for nucleic acid amplification, is housed beforehand; a wide-mouthed piping part 3a that communicates with the narrow-mouthed piping part 3b, is provided on the upper side of the narrow-mouthed piping part 3b ,and has a wider aperture than an aperture 3e of the narrow-mouthed piping part 3b; and a punchable film 3c formed from aluminum foil or the like, provided such that it partitions the interval between the wide-mouthed piping part 3a and the narrow-mouthed piping part 3b. By means of sealing the narrow-mouthed piping part 3b with the film 3c, evaporation or contamination of the reagent housed within the narrow-mouthed piping part 3b can be prevented. The aperture 3e of the narrow-mouthed piping 3b is provided at the center portion of a bottom portion 3d of the wide-mouthed piping part 3a. Furthermore, the narrow-mouthed piping part 3b and the wide-mouthed piping part 3a are integrally formed, and the film 3c is attached to the bottom portion 3d of the wide-mouthed piping part 3a.

(28) FIG. 5(b) is a drawing showing a state in which, in order to punch the film 3c of the reaction housing part 3 shown in FIG. 5(a) using the tip for punching 212 mounted on the nozzle head 50 mentioned below, it is positioned on the upper side thereof. FIG. 5(c) is a drawing showing a state in which, by lowering the tip for punching 212, the end is inserted into the narrow-mouthed piping part 3b, and the film 3c is punched. FIG. 5(d) is a drawing showing a reaction container 4 comprising; a sealing lid 251 exhibiting a state in which the plug portion 251a of the sealing lid 251 is fitted within the wide-mouthed piping part 3a of the housing part for reactions 3, in which the film 3c has been punched, and the film 3c that has been punched is pushed into the inner wall of the aperture of the narrow-mouthed piping part 3b by the pushing portion 251d provided on the end of the plug portion 251a, and the housing part for reactions 3.

(29) FIG. 6(a) and FIG. 6(b) are drawings showing a case where a linking portion 31.sub.1 is linked with the housing part for reactions 3 according to the second embodiment of the present invention.

(30) The plug portion 251a.sub.1 of the sealing lid 251 is fitted with the wide-mouthed piping part 3a of the housing part for reactions 3, which has a punched film 3c.sub.1, and the film 3c.sub.1 exhibits a state in which it is pushed into the inner wall of the narrow-mouthed piping part 3b.sub.1 by means of the pushing portion 251d.sub.1. Further, it exhibits a state in which the linking portion 31.sub.1 mentioned below is inserted into the cavity 251f.sub.1 of the sealing lid 251.sub.1.

(31) FIG. 6(a) is a drawing showing a state in which the linking portion 31.sub.i (here, i=1 for example) that downwardly protrudes from the horizontal plate 32a of the light guide stage 32 mentioned below, is indirectly linked with the housing part for reactions 3 via the sealing lid 251.sub.i which has transparency, that is mounted on the wide-mouthed piping part 3a.sub.i of the housing part for reactions 3, and the linking portion 31.sub.i is inserted into the cavity of the sealing lid 251.sub.i, and the end surface thereof is adhered to the bottom surface of the cavity of the sealing lid 251.sub.i. The housing part for reactions 3.sub.i comprises a wide-mouthed piping part 3a.sub.i, and a narrow mouthed piping part 3b.sub.i communicated with the wide-mouthed piping part 3a.sub.i and formed narrower than the wide-mouthed piping part 3a.sub.i. Furthermore, the narrow-mouthed piping part 3b.sub.i is dried beforehand, or a liquid state solution for amplification 3f.sub.i is housed beforehand. Here, the reagent for real-time amplification represents 70 L of a master mix (SYBR (registered trademark) Green Mix) consisting of enzymes, buffers, primers, and the like.

(32) For the aperture of the wide-mouthed piping part 3ai, in order to mount the sealing lid 251.sub.i which has transparency and protrudes on the lower side of the sealing lid 251.sub.i, onto the housing part for reactions 3.sub.i, and as a result of the circular pushing portion 251d.sub.i which encloses the center portion in which the light of the sealing lid 251.sub.i passes through, being inserted into the narrow-mouthed piping part, it is preferable for the diameter of the optical fiber (bundle) 33.sub.i which represents the light guide portion that passes through the linking portion 31.sub.i, to be the same or larger than the diameter of the aperture of the narrow-mouthed piping part 3b.sub.i. Consequently, it becomes possible to receive the light from the housing part for reactions 3.sub.i with certainty. The narrow-mouthed piping part 3b.sub.i is housed within a block for temperature control that is heated or cooled by means of the temperature controller 29.

(33) In this example, the optical fiber (bundle) 33.sub.i comprises an optical fiber (bundle) for irradiation 332.sub.i that is connectable with a second measuring end 43.sub.i, and an optical fiber (bundle) for receiving light 331.sub.i that is connectable with a first measuring end 42.sub.i.

(34) FIG. 6(b) is a drawing showing an example in which the optical fiber (bundle) 33.sub.i comprises an optical fiber bundle in which an optical fiber bundle comprising a plurality of optical fibers for receiving light that are connectable with the second measuring end 43.sub.i, and an optical fiber bundle comprising a plurality of optical fibers for irradiation that are connectable with the first measuring end 42.sub.i, are combined such that they become uniform.

(35) FIG. 7 is a housing part for reactions 5 according to a third embodiment of the present invention, and a processing explanatory drawing thereof.

(36) As shown in FIG. 7(b), the housing part for reactions 5 according to the third embodiment has: a narrow-mouthed piping part 5b, whereby a solution for nucleic acid amplification or a portion thereof 5f, which represents the reaction reagent for nucleic acid amplification, is housed beforehand; a wide-mouthed piping part 5a that communicates with the narrow-mouthed piping part 5b, is provided on the upper side of the narrow-mouthed piping part 5b, and has a wider aperture than an aperture 5e of the narrow-mouthed piping part 5b; and a punchable film 5c formed from aluminum foil or the like, provided such that it partitions the interval between the wide-mouthed piping part 5a and the narrow-mouthed piping part 5b.

(37) As shown disassembled in FIG. 7(a), the housing part for reactions 5 according to the third embodiment is different from the housing parts for reactions 1 and 3 according to the first and the second embodiment. In the housing part for reactions 5, the wide-mouthed piping part 5a and the narrow-mouthed piping part 5b are separately formed. A hole portion 5h is piercingly provided at the center of the bottom portion 5d of the wide-mouthed piping part 5a, and the narrow-mouthed piping part 5b has an aperture edge portion 5g along the outer circumference of its aperture 5e, that encloses the aperture 5e. The narrow-mouthed piping part 5b is provided such that, excluding the aperture edge portion 5g, it is able to pass through the hole portion 5h. The narrow-mouthed piping part 5b downwardly protrudes from the hole portion 5h of the wide-mouthed piping part 5a such that it passes through the hole portion 5h. The aperture edge portion 5g is mounted on the bottom portion 5d of the wide-mouthed piping part 5a, and the film 5c is attached to the aperture edge portion 5g of the narrow-mouthed piping part 5b. FIG. 7(c) is a drawing showing a state in which, in order to punch the film 5c of the reaction housing part 5 shown in FIG. 5(b) using the tip for punching 212 mounted on the nozzle head 50 mentioned below, it is positioned on the upper side thereof. FIG. 7(d) is a drawing showing a state in which, by lowering the tip for punching 212, the end is inserted into the narrow-mouthed piping part 5b, and the film 5c is punched.

(38) In order to manufacture the housing part for reactions 5 according to the third embodiment, as shown in FIG. 7(a), in step 1, the wide-mouthed piping part 5a, in which the hole portion 5h is piercingly provided in the center of the bottom portion 5d, is manufactured by means of blow molding or injection molding using P.P or P.E for example, and the narrow-mouthed piping part 5b, which has the aperture edge portion 5g along the outer circumference of the aperture 5e that encloses the aperture 5e, is separately manufactured by blow molding using P.P. or P.E. for example.

(39) In step 2, the reaction reagent or a portion thereof 5f is housed within the narrow-mouthed piping part 5b.

(40) In step 3, an adhesive is applied on the upper side of the aperture edge portion 5g of the narrow-mouthed piping part 5b, and the aperture 5e is sealed by attaching the punchable film 5c using heat sealing or ultrasonic sealing such that there are no gaps, and the reaction reagent or a portion thereof 5f is enclosed within the narrow-mouthed piping part 5b. The film is composed of an aluminum layer and a resin layer.

(41) In step 4, the narrow-mouthed piping part 5b, excluding its aperture edge portion 5g, downwardly protrudes such that it passes through the hole portion 5h of the wide-mouthed piping part 5a, and the housing part for reactions 5 is manufactured by mounting the lower side of the aperture edge portion 5g on the upper side of the bottom portion 5d of the wide-mouthed piping part 5a by applying an adhesive and attaching it by heat sealing or ultrasonic sealing such that there are no gaps. Here, a single-liquid moisture curing type multi-purpose elastic adhesive (HT-Bond Miracle 4 for example) is used as the adhesive for example.

(42) FIG. 8 is a drawing showing reaction containers 6 and 7 representing other examples using the housing part for reactions 5 according to the third embodiment.

(43) FIG. 8(a) is a drawing showing a sealing lid 252, whereby an O ring 252h is provided along the edge of the lower side of the plug portion 252a such that it encloses the pushing portion 252d. Consequently, in a case where the sealing lid 252 is fitted to the wide-mouthed piping part 5a of the housing part for reactions 5 via the plug portion 252a, the interval between the film 5c attached to the aperture edge portion 5g of the narrow-mouthed piping part 5b and the sealing lid is sealed, and processing without fluid leakage can be performed.

(44) FIG. 8B is a drawing showing a sealing lid 253, whereby a circular protrusion 253h is provided along the outer circumference of the end of the pushing portion 253d provided on the end of the plug portion 253a. Consequently, the film 5c, which is piercingly provided, is able to be pressed against the inner wall of the narrow-mouthed piping part 5b with certainty.

(45) FIG. 9 is a drawing showing a state in which the sealing lid 253.sub.i of the reaction container 7 shown in FIG. 8(b), and a linking portion 31i, are linked.

(46) FIG. 10 is a drawing showing a cartridge container 9 according to a fifth embodiment of the present invention, which further has a base plate 8j in which four concave portions (8, 8k, 8l and 8m) provided to the base plate 8j are arranged in a single row form. The housing part for reactions 8 is formed in the concave portion (8), the concave portion (8m) has a sealing lid housing part 8m that houses the sealing lid 253, the concave portion (8l) has a tip for punching housing part 8l that houses the tip for punching 212, and the concave portion (8k) has a dispensing tip housing part 8k that houses the dispensing tip 211.

(47) In order to manufacture the cartridge container 9, in step s1, as shown in FIG. 10, the base plate 8j, in which the four concave portions 8a, 8k, 8l, and 8m are arranged in a single row form and the hole portion 8h is piercingly provided in the center of the bottom portion 8d of the concave portion (wide-mouthed piping part) 8a, is manufactured by means of blow molding or injection molding, and the like, using P.P. or P.E. for example, and the narrow-mouthed piping part 8b, which has the aperture edge portion 8g along the outer circumference of the aperture 8e that encloses the aperture 8e, is separately manufactured by blow molding using P.P. or P.E. for example.

(48) In step s2, the reaction reagent or a portion thereof 8f is housed within the narrow-mouthed piping part 8b.

(49) In step s3, an adhesive is applied on the upper side of the aperture edge portion 8g of the narrow-mouthed piping part 8b, and the aperture 8e is sealed by attaching the punchable film 8c using heat sealing or ultrasonic sealing for example, such that there are no gaps, and the reaction reagent or a portion thereof 8f is enclosed within the narrow-mouthed piping part 8b. The film is composed of an aluminum layer and a resin layer.

(50) In step s4, the narrow-mouthed piping part 8b, excluding the aperture edge portion 8g thereof, downwardly protrudes such that it passes through the hole portion 8h of the wide-mouthed piping part 5a, and the housing part for reactions 8 is manufactured by mounting the lower side of the aperture edge portion 8g on the upper side of the bottom portion 8d of the wide-mouthed piping part 8a by applying an adhesive and attaching it using heat sealing or ultrasonic sealing such that there are no gaps. Here, a single-liquid moisture curing type multi-purpose elastic adhesive (HT-Bond Miracle 4 for example) is used as the adhesive for example.

(51) In step s5, the cartridge container 9 is manufactured by means of the dispensing tip 211, the tip for punching 212, and the sealing lid 253 being housed in the respective concave portions 8k, 8l, and 8m.

(52) FIG. 11 is a drawing showing a cartridge container 201.sub.1 according to a sixth embodiment of the present invention, wherein a partition wall 201.sub.0 is provided to the cartridge container 9 shown in FIG. 10 along the row direction (the movement path of the nozzles) in which the concave portions are arranged. This is represented by reference symbol 201.sub.1. Hereunder, a reaction container light measurement device 10 which represents a reaction container system utilizing the cartridge container 201.sub.1 is described.

(53) Hereunder, the reaction container light measurement device 10 mentioned above which represents a reaction container system according to a seventh embodiment of the present invention, is described more specifically with reference to FIG. 12 to FIG. 18. FIG. 12 is a see-through perspective view showing an external view of the reaction container light measurement device 10.

(54) FIG. 12(a) is a drawing showing an external view of the reaction container light measurement device 10, which has: an enclosure 11 with a size of 500 mm in depth (Y axis direction), 600 mm in width (X axis direction), and 600 mm in height (Z axis direction) for example, in which the container group 20, the nozzle head 50, a nozzle head transfer mechanism, and a CPU+program are housed in the interior; a control panel 13 provided on the enclosure 11; and a drawer 15 to which a stage is provided.

(55) FIG. 12(b) is a perspective view that sees through the interior of the enclosure 11, wherein the stage, into which the container group 20 is built-in, is able to be drawn out to the exterior by means of the drawer 15, and further, the nozzle head 50 is movably provided in the X axis direction with respect to the container group 20 by means of the nozzle head transfer mechanism.

(56) FIG. 12(b) is a drawing showing that the nozzle head 50 is largely provided with: various transfer mechanisms 52 having an arranging body Y axis transfer mechanism 41, a stage Z axis transfer mechanism 35, and a nozzle Z axis transfer mechanism 75; a traversable nozzle suction-discharge mechanism 17; the measuring device 40; a connecting end arranging body 30; an optical fiber (bundle) 33.sub.i; and the magnetic force part 57. The traversable nozzle suction-discharge mechanism 17 and the traversable nozzles 71.sub.0 are supported such that they are movable in the Y axis direction by means of the arranging body Y axis transfer mechanism 41 such that they traverse the exclusive regions 20.sub.i.

(57) FIG. 13 is a plan view showing enlarged, the container group 20 shown in FIG. 12. The container group 20 is one in which twelve exclusive regions 20i (i=1, . . . 12), wherein the longitudinal direction thereof is along the X axis direction and housing parts are arranged in a single row form, are arranged in parallel along the Y axis direction at a pitch of 18 mm for example. The exclusive regions 20.sub.i are separately provided with a cartridge container for PCR amplification 201.sub.i, a cartridge container for nucleic acid extraction 202.sub.i, and a cartridge container for housing tips 203.sub.i according to the sixth embodiment of the present invention. The prevention of cross-contaminations between the exclusive regions 20.sub.i is achieved by providing partition walls 201.sub.0, 202.sub.0, and 203.sub.0 on the cartridge containers 201.sub.i, 202.sub.i, and 203.sub.i of the exclusive regions 20.sub.i on the edge of one side along the X axis direction.

(58) The cartridge container for PCR amplification 201.sub.1 has: the housing part for reactions 8.sub.i that, in addition to being detachably linked with the twelve linking portions 31.sub.i provided to the light guide stage 32 via a single sealing lid 253.sub.i which has transparency, houses beforehand the solutions for nucleic acid amplification such as a buffer solution necessary for the PCR reaction; sealing lid housing parts 25.sub.i housing the sealing lids 253.sub.i; tips for punching 212.sub.i for punching the punchable film 8c covering the housing parts for reactions 8.sub.i and the narrow-mouthed piping parts 8b.sub.i; and housing parts for tips and the like 21.sub.i that respectively house the dispensing tips 211.sub.i. It is preferable to provide a barcode that displays the sample information and the inspection information relating to the cartridge container for PCR amplification 201.sub.i.

(59) The cartridge container for nucleic acid extraction 202.sub.i has: seven liquid housing parts 272.sub.i for example, that house various reagents for nucleic acid extraction; tubes for reactions Y 232.sub.i that house the extracted nucleic acids; and barcodes 82.sub.i that display various information, such as the sample information and the inspection information, related to the cartridge container. The housing parts for reactions 8.sub.i and the tubes for reactions 232.sub.i are temperature controllable by means of the temperature controller 29.

(60) The cartridge container for housing tips 203.sub.i has: a tip for punching that is able to punch the film covering the cartridge container for nucleic acid extraction 202.sub.i; two small-quantity dispensing tips that perform the dispensing of small quantities of liquids; and housing parts for tips and the like 21.sub.i that house dispensing tips for separations that are able to perform separation by adsorbing magnetic particles on an inner wall by applying and removing a magnetic force from the exterior. It is preferable to provide a barcode that display various information relating to the cartridge container 203.sub.i.

(61) The capacity of the housing part for reactions 8.sub.i is of the order of approximately 200 L, and the capacity of the other reaction containers, liquid housing parts, and tubes is of the order of approximately 2 mL.

(62) The housing part for reactions 8.sub.i is used for the amplification of nucleic acids or the fragments thereof, and temperature control is performed by means of the temperature controller 29 based on a predetermined amplification method, such as a thermal cycle (from 4 C. to 95 C.) for example. The housing part for reactions 8.sub.i is formed with two levels as shown in FIG. 10(b) for example, and has a narrow-mouthed piping part 8b.sub.i provided on the lower side in which the solution for amplification 8f.sub.i is housed, and a wide-mouthed piping part 8a.sub.i provided on the upper side in which the sealing lid 253.sub.i is fittable. The inner diameter of the wide-mouthed piping part 8a.sub.iis 8 mm for example, and the inner diameter of the aperture of the narrow-mouthed piping part 8b.sub.i is approximately 5 mm for example. The tubes for reactions 232.sub.i housed in the reaction tube housing holes are temperature controlled for incubation to a constant temperature of 55 C. for example.

(63) The liquid housing part group 272.sub.i houses the solutions for separating and extracting as follows. A first liquid housing part houses 40 L of Lysis 1, a second liquid housing part houses 200 L of Lysis 2, a third liquid housing part houses 500 L of a binding buffer solution, a fourth liquid housing part houses a magnetic particle suspension, a fifth liquid housing part houses 700 L of the washing liquid 1, a sixth liquid housing part houses 700 L of the washing liquid 2, a seventh liquid housing part houses 50 L of distilled water as a dissociation liquid, and an eighth liquid housing part, which is slightly separated, houses 1300 L of isopropyl alcohol (isopropanol) used for the removal of protein and the like, as a portion of the solution for separating and extracting protein. The respective reagents and the like are prepacked as a result of the punchable film covering the respective apertures thereof.

(64) In addition, 1.2 mL of distilled water is housed in a separate distilled water reservoir, and tubes that house suspensions of bacteria, cells, and the like, or samples such as whole blood, are separately prepared for each of the respective exclusive regions 20.sub.i.

(65) FIG. 14 is a front view and a side view of the nozzle head 50 according to the seventh embodiment of the present invention, and FIG. 15 is a perspective view from the front side.

(66) The nozzle head 50 is one having: a nozzle arranging portion 70 in which twelve nozzles 71.sub.i are arranged; a tip detaching mechanism 59 that is able to detach dispensing tips 211.sub.i mounted on the nozzles 71.sub.i; a suction-discharge mechanism 53; a magnetic force part 57 having twelve magnets 571 provided such that they are able to approach and separate with respect to the dispensing tips 211.sub.i; a light guide stage 32; twelve linking portions 31.sub.i provided to the light guide stage 32; a transfer mechanism portion 52 having a nozzle Z axis transfer mechanism 75 and a stage Z axis transfer mechanism 35; optical fibers (bundles) 33.sub.i representing flexible light guide portions that extend to the rear side from the linking portions 31.sub.i; a connecting end arranging body 30; the arranging body Y axis transfer mechanism 41; a measuring device 40 having a measuring end 44; a traversable nozzle 71.sub.0; and a suction-discharge mechanism 17 thereof.

(67) The nozzle arranging portion 70 is provided with a cylinder supporting member 73 that supports twelve cylinders 531.sub.i such that they are arranged along the Y axis direction at the predetermined pitch of 18 mm for example. The nozzles 71.sub.i are provided on the downward end of the cylinders 531.sub.i such that they are communicated with the cylinders 531.sub.i.

(68) The tip detaching mechanism 59 is provided with detaching shafts 593 on both sides, and has a tip detaching member 591 that detaches the twelve dispensing tips 211.sub.i from the nozzles 71.sub.i by sliding in the vertical direction.

(69) As shown specifically in FIG. 16 and FIG. 17, the tip detaching member 591 is interlocked with the lowering of two tip detaching shafts 593 and detaches the dispensing tips 211.sub.i from the nozzles 71.sub.i. The tip detaching shaft 593 is elastically supported by the cylinder support member 73 by means of a spring 600 wrapped around the outer periphery such that it is biased in the upward direction, and the upper end thereof is positioned above the upper ends of the cylinders 531.sub.i but below the lower limit position of the vertical movement range of the normal suction and discharge of a cylinder drive plate 536 mentioned below. The two tip detaching shafts 593 are pushed in the downward direction by means of the cylinder drive plate 536 exceeding the vertical movement range and being lowered near the upper end of the cylinder 531.sub.i, thus lowering the tip detaching member 591. The tip detaching member 591 has twelve holes having an inner diameter that is larger than the outer diameter of the nozzles 71.sub.i but smaller than the mounting portions 211.sub.ic, which represents the largest outer diameter of the dispensing tips 211.sub.i, arranged at the pitch mentioned above such that the nozzles 71.sub.i pass therethrough.

(70) As shown specifically in FIG. 16 and FIG. 17, the suction-discharge mechanism 53 has: the cylinders 531.sub.i for performing suction and discharge of gases with respect to the dispensing tips 211.sub.i which are communicated with the nozzles 71.sub.i and mounted on the nozzles 71.sub.i, and a piston rod 532 that slides within the cylinders 531.sub.i; a drive plate 536 that drives the piston rod 532; a ball screw 533 that threads with the drive plate 536; a nozzle Z axis movable body 535 that, in addition to axially supporting the ball screw 533, is integrally formed with the cylinder support member 73; and a motor 534 mounted on the nozzle Z axis movable body 535 that rotatingly drives the ball screw 533.

(71) The magnetic force part 57 has a magnet 571 that is provided such that it can approach and separate with respect to the narrow diameter portions 211.sub.ia of the dispensing tips 211.sub.i detachably mounted on the nozzles 71.sub.i, and is able to apply and remove a magnetic field in the interior of the dispensing tips 211.sub.i.

(72) As shown specifically in FIG. 16, the nozzle Z axis transfer mechanism 75 has: a ball screw 752 that threads with the Z axis movable body 535 and vertically moves the Z axis movable body 535 along the Z direction; a nozzle head substrate 753 that axially supports the ball screw 752, and in addition to axially supporting the magnet 571 on the lower side thereof such that it is movable in the X axis direction, is itself movable in the X axis direction by means of the nozzle head transfer mechanism 51 mentioned below; and a motor 751 provided on the upper side of the nozzle head substrate 753 that rotatingly drives the ball screw 752.

(73) As shown specifically in FIG. 16, the light guide stage 32 comprises a horizontal plate 32a and a vertical plate 32b, which are letter-L shaped plates in cross-section, and is provided with twelve cylinder-shaped linking portions 31.sub.i having ends of optical fibers (bundles) 33.sub.i, which are directly or indirectly linkable with the apertures of the PCR tubes 231.sub.i and are optically connected with the interior of the linked PCR tubes 231.sub.i, protruding in the downward direction from the horizontal plate 32a. Furthermore, a heater 37 that heats the sealing lids 251.sub.i mounted on the linking portions 31.sub.i and prevents condensation, is built into the bases of the linking portions 31.sub.i. The temperature of the heater is set to approximately 105 C. for example. Since the light guide stage 32 is supported by the nozzle head substrate 753 by means of the nozzle head stage Z axis transfer mechanism 35 such that it is movable in the Z axis direction, it is movable in the nozzle X axis direction and Z axis direction.

(74) The stage Z axis transfer mechanism 35 has: a side plate 355 provided on the nozzle head substrate 753; a mount driving band-shaped member 354 that is supported by a timing belt 352 spanning between two sprockets 353 arranged in the vertical direction axially supported by the side plate 355, and vertically moves in the Z axis direction; and a motor attached to the rear side of the nozzle head substrate 753 that rotatingly drives the sprockets 353.

(75) As shown in FIG. 17, the traversable nozzle suction-discharge mechanism 17 is provided with a tip detaching mechanism 592 on the lower side of the suction-discharge mechanism 17 and on the upper side of the nozzle 71.sub.0. Furthermore, the suction-discharge mechanism 17 is provided with a digital camera 19. The suction-discharge mechanism 17 is movably provided in the Y axis direction by being attached to a timing belt 171 spanning between two sprockets 173 that are rotatingly driven by a motor 172.

(76) FIG. 18 represents two perspective views of the nozzle head according to the seventh embodiment viewed from the rear side, which show the connection starting position (FIG. 18(a)) and the connection finishing position (FIG. 18(b)) at the time the respective connecting ends of the connecting end arranging body 30 and the respective measuring ends are successively optically connected.

(77) The linking portions 31.sub.i are provided with the ends of the optical fibers (bundles) 33.sub.i, and have: a connecting end arranging body 30 in which the connecting ends 34.sub.i which pass through the horizontal plate 32a of the light guide stage 32, and the rear ends thereof provided corresponding to the respective linking portions 31.sub.i, are arranged on an arranging surface on a path along a straight line in the Y axis direction, which represents a predetermined path, at a shorter spacing than the spacing of the linking portions 31.sub.i; and six measuring ends that are provided in the vicinity of, or making contact with, the arranging surface, and are successively optically connectable with the connecting ends 34.sub.i along the straight line. There is also provided a measuring device 40 in which, by means of optical connections between the connecting ends and the measuring ends, the fluorescent light within the housing parts for reactions 8.sub.i, which represents the optical state, is receivable, and excitation light is also able to be irradiated.

(78) Furthermore, the light guide stage 32 has a cylinder-shaped body 311.sub.i, which retains the optical fibers (bundles) 33.sub.i extending to the rear side from the linking portions 31.sub.i such that they pass through the interior in order to prevent folding, protrudingly provided upward from the horizontal plate 32a directly above the linking portions 31.sub.i. In the same manner, the connecting end arranging body 30 is also provided with a cylinder-shaped body 301.sub.i, which retains the optical fibers (bundles) 33.sub.i extending from the connecting ends 34.sub.i such that they pass through the interior in order to prevent folding, on the connecting end 34.sub.i side.

(79) The arranging body Y axis transfer mechanism 41 that moves the connecting end arranging body 30 in the Y axis direction has: arms 412 and 413 provided to the connecting end arranging body 30; a joining body 411 that joins the arms 412 and 413 and the timing belt; a guide rail 414 that guides the Y axis movement of the joining body 411; and two sprockets spanned by the timing belt and arranged along the Y axis direction.

(80) The measuring device 40 is one that supports the measurement of fluorescent light and comprises six types of specific wavelength measuring devices 40.sub.j that are linearly aligned along a straight line in the Y axis direction, which represents the predetermined path, such that they support the measurement of six types of fluorescent light, and they are provided fixed on a substrate of the nozzle head 50, such as the frame that encloses the transfer mechanism portion 52, or a member that supports the same. Therefore, depending on the mechanism provided to the transfer mechanism portion 52, the measuring device 40 does not move.

(81) The measuring device 40 is one in which the measuring ends of the plurality of types (six in this example) of specific wavelength measuring devices 40.sub.j (j=1, 2, 3, 4, 5, 6), and therefore, in this case, the specific wavelength measuring devices 40.sub.j themselves are aligned in a single row form, and integrally fixed to a member joined with the nozzle head substrate 753 using fixtures 45.sub.j. The specific wavelength measuring devices 40.sub.j have: measuring ends 44.sub.j arranged along a straight line path in the Y axis direction which represents the predetermined path, such that they successively optically connect to the connecting ends 34.sub.i; light detectors 46.sub.j in which an optical system having an irradiation source that irradiates excitation light to the PCR tubes 231.sub.i and a light receiving portion that receives the fluorescent light generated in the housing part for reactions 8.sub.i are built-in; and circuit boards 47.sub.j. The measuring ends 44.sub.j have first measuring ends 42.sub.j that optically connect with the irradiation source, and second measuring ends 43.sub.j that optically connect with the light receiving portion. Here, the light detectors 46.sub.j and the circuit boards 47.sub.j correspond to the measuring device main body.

(82) The pitch between the respective connecting ends 34.sub.i, assuming a pitch between the linking portions 31.sub.i of 18 mm, is 9 mm, which is half thereof. Then, the pitch between the measuring ends 44.sub.j is 9 mm or less for example.

(83) There is a case where the first measuring ends 42.sub.j and the second measuring ends 43.sub.j of the measuring ends 44.sub.j of the respective specific wavelength measuring devices 40.sub.j are arranged aligned in a lateral direction (Y axis direction) along the straight line of the Y axis direction along the predetermined path, and a case where they are arranged aligned in a longitudinal direction (X axis direction). In the former case, without stopping the emission of the excitation light, the respective measuring devices successively receive light at a timing for receiving light determined based on the speed of the connecting end arranging body, the pitch between the connecting ends, the distance between the first measuring ends and the second measuring ends of the measuring ends, and the pitch between the measuring ends.

(84) On the other hand, in the latter case, as shown in FIG. 18, with respect to the connecting end, a first connecting end and a second connecting end are provided. The first connecting end connects only with the first measuring ends 42.sub.j, and the second measuring ends 43.sub.j connect only with the second connecting end. The fixed path represents two paths. Furthermore, the optical fibers (bundles) 33.sub.i have optical fibers (bundles) 331.sub.i for receiving light that have the first connecting end, and optical fibers (bundles) 332.sub.i for irradiation that have the second connecting end. In this case, compared to the former case, connection with the linking portions is performed by means of optical fibers in which the irradiation source and the light receiving portion are dedicated, and therefore, the control is simple, and the reliability is high since optical fibers that are respectively suitable for irradiation and receiving light can be used.

(85) The speed of the connecting end arranging body 30 with respect to the measuring ends 44.sub.j is determined with consideration of the stable light receivable time, the lifetime of the fluorescent light with respect to excitation light irradiation, the number of connecting ends, the pitch between the connecting ends, and the like (the distance of the predetermined path). In the case of a real-time PCR measurement, it is controlled such that it becomes 100 mm to 500 mm per second for example. In the present embodiment, since the movement is performed by sliding the arranging surface with respect to the measuring ends 44, the incidence of optical noise to the measuring ends 44 can be prevented. Furthermore, the connecting end arranging body 30 moves with respect to the measuring ends intermittently such that it momentarily stops at each pitch advance between the connecting ends or between the measuring ends, or continuously.

(86) Next, a series of processing operations that perform real-time PCR of the nucleic acids of a sample containing bacteria using the reaction container light measurement device 10 according to the seventh embodiment is described. Step S1 to step S13 below correspond to separation and extraction processing.

(87) In step S1, the drawer 15 of the reaction container light measurement device 10 shown in FIG. 12 is opened, the container group 20 is pulled out, and by utilizing a feeding device for samples and the like, which is provided separately from the container group 20, the samples which are subject to testing, various washing liquids, and various reagents, are supplied beforehand, and furthermore, a liquid housing part in which reagents and the like are prepacked is mounted.

(88) In step S2, following returning of the container group 20 and closing of the drawer 15, the start of the separation and extraction and amplification processing is instructed by means of the operation of the touch panel of the control panel 13 for example.

(89) In step S3, the extraction control part provided to the nucleic acid processing controller of the CPU+program of the reaction container light measurement device 10 instructs the nozzle head transfer mechanism 51 and moves the nozzle head 50 in the X axis direction, positions the tip for punching mounted to the nozzle 71.sub.i above the first liquid housing part of the liquid housing part group 27.sub.i of the container group, and punches the film covering the aperture of the liquid housing part by lowering the nozzle by means of the nozzle Z axis transfer mechanism 75, and in the same manner, the other liquid housing parts of the liquid housing part group 27.sub.i and the reaction container group 23.sub.i are successively punched by moving the nozzle head 50 in the X axis direction.

(90) In step S4, the nozzle head 50 is again moved in the X axis direction and moved to the housing part for tips and the like 21.sub.i, and the nozzles 71.sub.i are lowered by means of the nozzle Z axis transfer mechanism 75, and the dispensing tips 211.sub.i are mounted. Next, after being raised by the nozzle Z axis transfer mechanism 75, the dispensing tips 211.sub.i are moved along the X axis by means of the nozzle head transfer mechanism 51, and advanced to the eighth liquid housing part of the liquid housing part group 27.sub.i. Then a predetermined amount of isopropanol is aspirated from the liquid housing part, and they are again moved along the X axis direction, and predetermined amounts are respectively dispensed into the solution components (NaCl, SDS solutions) housed in the third liquid housing part and the fifth liquid housing part, and the distilled water housed in the sixth liquid housing part, so that 500 L of a binding buffer solution (NaCl, SDS, isopropanol), 700 L of a washing liquid 1 (NaCl, SDS, isopropanol), and 700 L of a washing liquid 2 (water 50%, isopropanol 50%) are respectively prepared as solutions for separating and extracting within the third, the fifth, and the sixth liquid housing parts.

(91) In step S5, following movement to, among the housing parts for tips and the like 21.sub.i, the sample tube in which the sample is separately housed, the narrow diameter portion 211.sub.ia of the dispensing tip 211.sub.i is loweringly inserted using the nozzle Z axis transfer mechanism 75, and, with respect to the suspension of the sample housed in the sample tube, following suspension of the sample within the liquid by repeating the suction and the discharge by raising and lowering the drive plate 536 of the suction-discharge mechanism 53, the sample suspension is aspirated within the dispensing tip 211.sub.i. The sample suspension is moved along the X axis by means of the nozzle head transfer mechanism 51 to the first liquid housing part of the liquid housing part group 27.sub.i housing the Lysis 1 (enzyme) representing the solution for separating and extracting, and the narrow diameter portion 211.sub.ia of the dispensing tip 211.sub.i is inserted through the hole in the punched film, and the suction and the discharge is repeated in order to stir the sample suspension and the Lysis 1.

(92) In step S6, the entire amount of the stirred liquid is aspirated by the dispensing tip 211.sub.i, and incubation is performed by housing it in the reaction tube retained in the storage cavity 232.sub.i that is set to 55 C. by means of the constant temperature controller. Consequently, the protein contained in the sample is broken down and made a low molecular weight. After a predetermined time has elapsed, the reaction mixture is left in the reaction tube, the dispensing tip 211.sub.i is moved to the second liquid housing part of the liquid housing part group 27.sub.i by means of the nozzle head transfer mechanism 51, and the entire amount of the liquid housed within the second liquid housing part is aspirated by using the nozzle Z axis transfer mechanism 75 and the suction-discharge mechanism 53, and it is transferred using the dispensing tip 211.sub.i by means of the nozzle head transfer mechanism 51, and the reaction solution is discharged within the third liquid housing part by penetrating the hole in the film and inserting the narrow diameter portion.

(93) In step S7, the binding buffer solution housed within the third liquid housing part, which represents a separation and extraction solution, and the reaction solution are stirred, the solubilized protein is further dehydrated, and the nucleic acids or the fragments thereof are dispersed within the solution.

(94) In step S8, using the dispensing tip 211.sub.i, the narrow diameter portion thereof is inserted into the third liquid housing part by passing through the hole in the film, the entire amount is aspirated and the dispensing tip 211.sub.i is raised by means of the nozzle Z axis transfer mechanism 75, and the reaction solution is transferred to the fourth liquid housing part, and the magnetic particle suspension housed within the fourth liquid housing part is stirred with the reaction solution. A cation structure in which Na+ ions bind to the hydroxyl groups formed on the surface of the magnetic particles contained within the magnetic particle suspension is formed. Consequently, the negatively charged DNA is captured by the magnetic particles.

(95) In step S9, the magnetic particles are adsorbed on the inner wall of the narrow diameter portion 211.sub.ia of the dispensing tip 211.sub.i by approaching the magnet 571 of the magnetic force part 57 to the narrow diameter portion 211.sub.ia of the dispensing tip 211.sub.i. In a state in which the magnetic particles are adsorbed on the inner wall of the narrow diameter portion 211.sub.ia of the dispensing tip 211.sub.i, the dispensing tip 211.sub.i is raised by means of the nozzle Z axis transfer mechanism 75 and moved from the fourth liquid housing part to the fifth liquid housing part using the nozzle head transfer mechanism 51, and the narrow diameter portion 211.sub.ia is inserted by passing through the hole in the film.

(96) In a state in which the magnetic force within the narrow diameter portion 211.sub.ia is removed by separating the magnet 571 of the magnetic force part 57 from the narrow diameter portion 211.sub.ia of the dispensing tip 211.sub.i, by repeating the suction and the discharge of the washing liquid 1 (NaCl, SDS, isopropanol) housed in the fifth liquid housing part, the magnetic particles are released from the inner wall, and the protein is washed by stirring within the washing liquid 1. Thereafter, in a state in which the magnetic particles are adsorbed on the inner wall of the narrow diameter portion 211.sub.ia as a result of approaching the magnet 571 of the magnetic force part 57 to the narrow diameter portion 211.sub.ia of the narrow diameter portion 211.sub.ia again, the dispensing tip 211.sub.i is, by means of the nozzle Z axis transfer mechanism 75, moved from the fifth liquid housing part to the sixth liquid housing part by means of the nozzle head transfer mechanism 51.

(97) In step S10, the narrow diameter portion 211.sub.ia of the dispensing tip 211.sub.i is inserted by passing through the hole in the film using the nozzle Z axis transfer mechanism 75. By repeating the suction and the discharge of the washing liquid 2 (isopropanol) housed in the sixth liquid housing part in a state in which the magnetic force within the narrow diameter portion 211.sub.ia is removed by separating the magnet 571 of the magnetic force part 57 from the narrow diameter portion 211.sub.ia of the dispensing tip 211.sub.i, the magnetic particles are stirred within the liquid, the NaCl and the SDS is removed, and the protein is washed. Thereafter, in a state in which the magnetic particles are adsorbed on the inner wall of the narrow diameter portion 211.sub.ia by approaching the magnet 571 of the magnetic force part 57 to the narrow diameter portion 211.sub.ia of the dispensing tip 211.sub.i again, the dispensing tip 211.sub.i is, following raising by means of the nozzle Z axis transfer mechanism 75, moved from the sixth liquid housing part to the seventh liquid housing part in which the distilled water is housed, by means of the nozzle head transfer mechanism 51.

(98) In step S11, the narrow diameter portion 211.sub.ia of the dispensing tip 211.sub.i is lowered through the hole by means of the nozzle Z axis transfer mechanism 75, and by repeating the suction and the discharge of the water at a slow flow rate in a state where the magnetic force is applied within the narrow diameter portion 211.sub.ia of the dispensing tip 211.sub.i, the isopropanol is substituted by water and is removed. Thereafter, by stirring the magnetic particles by repeating the suction and the discharge within the distilled water which represents the dissociation liquid, in a state in which the magnet 571 of the magnetic force part 57 is separated from the narrow diameter portion 211.sub.ia of the dispensing tip 211.sub.i and the magnetic force is removed, the nucleic acids or the fragments thereof retained by the magnetic particles are dissociated (eluted) from the magnetic particles into the liquid. Thereafter, a magnetic field is applied within the narrow diameter portion and the magnetic particles are adsorbed on the inner wall by approaching the magnet 571 to the narrow diameter portion 211.sub.ia of the dispensing tip 211.sub.i, and the solution containing the extracted nucleic acids, and the like, is made to remain in the eighth liquid housing part. The dispensing tip 211.sub.i is moved to the storage part of the housing parts for tips and the like 21.sub.i in which the dispensing tip 211.sub.i was housed, by means of the nozzle head transfer mechanism 51, and the dispensing tip 211.sub.i to which magnetic particles are adsorbed, is detached from the nozzle 71.sub.i together with the magnetic particles and dropped into the storage part, using the detaching member 591 of the tip detaching mechanism 59.

(99) The following step S12 to step S15 corresponds to nucleic acid amplification and measurement processing.

(100) In step S12, a new dispensing tip 211.sub.i is mounted on the nozzle 71.sub.i, the solution housed within the eighth liquid housing part, which contains nucleic acids and the like, is aspirated, and by transferring it to the housing part for reactions 8.sub.i, in which the solution for amplification 8f.sub.i is housed beforehand, and discharging it, it is introduced into the container. As a result of moving the nozzle head 50 by means of the nozzle head transfer mechanism 51, the nozzle 71.sub.i is moved above the sealing lid housing part 8m.sub.i of the container group 20, which houses the sealing lid 253.sub.i. Mounting is performed by lowering using the nozzle Z axis transfer mechanism 75 and fitting the cavity 253f on the upper side of the sealing lid 253 and fitting it to the lower end of the nozzle 71.sub.i. After being raised by the nozzle Z axis transfer mechanism 75, the sealing lid 253 is positioned above the housing part for reactions 8.sub.i using the nozzle head transfer mechanism 51, and by lowering the sealing lid 253 by means of the nozzle Z axis transfer mechanism 75, it is fitted with the aperture of the wide-mouthed piping part 8a.sub.i of the housing part for reactions 8.sub.i, mountingly sealing it.

(101) In step S13, the nozzle head transfer mechanism 51 is instructed by means of an instruction from the measurement control portion, and by moving the nozzle head 50 along the X axis, the linking portion 31.sub.i of the light guide stage 32 is positioned above the housing part for reactions 8.sub.i, which is mounted with the sealing lid 253.sub.i. Then, by lowering the light guide stage 32 by means of the stage Z axis transfer mechanism 35, the linking portion 31.sub.i is inserted into the cavity 253f.sub.i of the sealing lid 253.sub.i, and the lower end thereof is made to make contact with, or adhere to, the bottom surface 253g.sub.i of the cavity.

(102) In step S14, due to an instruction by the nucleic acid processing controller, the temperature controller 29 instructs a temperature control cycle by real-time PCR, such as a cycle in which the housing part for reactions 8.sub.i is heated for five seconds at 96 C. and heated for 15 seconds at 60 C., to be repeated forty nine times for example.

(103) In step S15, when temperature control at each cycle by the nucleic acid processing controller is started, the measurement control portion determines the start of elongation reaction processing at each cycle, and instructs the continuous or intermittent movement of the connecting end arranging body 30 with respect to the measuring ends 44.sub.j of the measuring device 40. For the movement speed thereof, it is moved at a speed that is calculated based on the stable light receivable time, the fluorescence lifetime, and the number (twelve in this example) of exclusive regions 20.sub.i. Consequently, the receiving of light from all twelve housing parts for reactions 8.sub.i within the stable light receivable time becomes completed.

(104) In step S16, the measurement control portion determines the moment of each optical connection between the optical fibers (bundles) 33.sub.i of the linking portions 31.sub.i and the first measuring end and the second measuring end of the measuring end 44, and instructs the receiving of light to the measuring device 40 for example.

(105) This measurement is executed with respect to cycles in which exponential amplification is performed, and an amplification curve is obtained based on the measurement, and various analyses are performed based on the amplification curve. At the time of the measurement, the measurement control portion heats the heater 37 built into the light guide stage 32 and prevents the condensation on the sealing lid 253, and a clear measurement can be performed.

(106) The foregoing embodiments have been specifically described in order to better understand the present invention, and they are in no way limiting of other embodiments. Therefore, modifications are possible within a scope that does not depart from the gist of the invention. The configurations, shapes, materials, arrangements, and amounts of the nozzles, the dispensing tips, the punching tips, the container group, the exclusive regions thereof, the housing parts, the housing parts for reactions, the wide-mouthed piping part, the narrow-mouthed piping part, the measuring ends, the measuring devices, the specific wavelength measuring devices, the suction-discharge mechanism, the transfer mechanism portion, the magnetic force part, the heating portion, the reaction container, the sealing lids, the light guide stage, the linking portions, the light guide portions, the connecting ends, the connecting end arranging body, the linking portion arranging body, the nozzle head, the temperature controller, and the like, and the utilized reagents and samples are also in no way limited by the examples illustrated in the embodiments. Furthermore, although the nozzles were made to move with respect to the container group, it is possible to also move the container group with respect to the nozzles.

(107) Furthermore, in the foregoing descriptions, although a sealing lid was used for the sealing of the housing part for reactions, it is possible, in its place or in combination, to perform sealing using a sealing liquid, such as mineral oil. Moreover, in place of punching by mounting a tip for punching on the nozzle, it is also possible to use a punching pin that is driven by the suction-discharge mechanism. Moreover, in the foregoing descriptions, although a real-time PCR measurement was described, it is in no way limited to this measurement, and it may also be applied to a variety of other measurements in which temperature control is performed. In the foregoing descriptions, although a case where the measuring device is provided to a dispensing device was described, it is not necessarily limited to this.

(108) Furthermore, the devices described in the respective exemplary embodiments of the present invention, the components that form these devices, or the components that form these components, can be appropriately selected, and can be mutually combined by applying appropriate modifications. The spatial representations within the present application, such as above, below, interior, exterior, X axis, Y axis, and Z axis are for illustration only, and are in no way limiting of the specific spatial directions or positions of the construction.

INDUSTRIAL APPLICABILITY

(109) The present invention is related to fields in which the processing, testing, and analysis of nucleic acids, which primarily includes DNA, RNA, mRNA, rRNA, and tRNA for example, is required, and is related to industrial fields, agricultural fields such as food, agricultural products, and fishery processing, chemical fields, pharmaceutical fields, health care fields such as hygiene, insurance, diseases, and genetics, and scientific fields such as biochemistry or biology for example. The present invention is, in particular, able to be used in processing and analysis that handles various nucleic acids, and the like, such as PCR and real-time PCR.

BRIEF DESCRIPTION OF THE REFERENCE SYMBOLS

(110) 1, 3, 5, 8 Housing part for reactions 1a, 3a, 5a, 8a Wide-mouthed piping part 1b, 3b, 5b, 8b Narrow-mouthed piping part 1c, 3c, 5c, 8c Punchable film 1f, 3f, 5f, 8f Reaction reagent 2, 4, 6, 7, 9, 201 Reaction container 10 Reaction container light measurement device 20 Container group 20.sub.i (i=1, . . . , 12) Exclusive regions 211.sub.i (i=1, . . . , 12) Dispensing tip 212 Tip for punching 231.sub.i, 236.sub.i (i=1, . . . , 12) PCR tube (reaction container) 251, 252, 253 Sealing lid 251a, 252a, 253a Plug portion 251b, 252b, 253b Protrusion 251c, 252c, 253c Cylindrical member 251d, 252d, 252d Pushing portion 251e, 252e, 252e Circular protrusion 251f, 252f, 253f Cavity 251g, 252g, 253g Bottom of cavity 252h O ring 253h Circular protrusion 29 Temperature controller 30 Connecting end arranging body 31.sub.i, 141.sub.i (i=1, . . . , 12) Linking portion 32 Light guide stage 33.sub.i, 142.sub.i Optical fiber (light guide portion) 40, 140 Measuring device 40.sub.j (j=1, . . . , 6) Specific wavelength measuring device 44 Measuring end 50 Nozzle head 52 Transfer mechanism portion 53 Suction-discharge mechanism 59 Tip detaching mechanism 70 Nozzle arranging portion 71.sub.i (i=1, . . . , 12) Nozzles