COMPOUNDS FOR TREATING PARASITIC INFECTIONS

Abstract

Compounds and compositions comprising them are provided. The compounds and compositions are useful for inhibiting transport of heme across membranes in parasitic heme auxotrophic organisms, thereby limiting their growth or killing the parasites.

Claims

1. A method for inhibiting transport of heme across a membrane in a parasitic heme auxotrophic organism comprising contacting the parasitic organism with an effective amount of compound having the following structure: ##STR00054## wherein R.sup.1 is selected from the group consisting of OH, OMe, and NHR.sup.6, wherein R.sup.6 is selected from the group consisting of C.sub.1-C.sub.5 alkyl, C.sub.3-C.sub.6 cycloalkyl, substituted or unsubstituted pyridine, and phenyl; R.sup.2 is selected from the group consisting of H, F, Cl, OMe, and OH; R.sup.3 is selected from the group consisting of C4-C6 cycloalklyl, phenyl, and substituted or unsubstituted pyridine; R.sup.4 is selected from the group consisting of substituted or unsubstituted C.sub.1-C.sub.8 alkyl, C.sub.4-C.sub.6 cycloalklyl, substituted or unsubstituted phenyl, substituted or unsubstituted alkyl amine, naphthyl, and heterocyclic; R.sup.5 is selected from the group consisting of ##STR00055## R.sup.7 is selected from the group consisting of halogen, phenyl, and alkyl; n is 0-5; Y is selected from the group consisting of H, F, Cl, and -Me; and Z is O, or NR.sup.8 wherein R.sup.8 is ##STR00056## and R.sup.9 is selected from the group consisting of OMe, and F, such that the transport of the heme across the membrane is inhibited or prevented.

2. The method of claim 1, wherein the compound has the following structure: ##STR00057## wherein R.sup.1 is selected from the group consisting of OH, OMe, and NHR.sub.6, wherein R.sup.6 is selected from the group consisting of C.sub.1-C.sub.5 alkyl, C.sub.3-C.sub.6 cycloalkyl, substituted or unsubstituted pyridine, and phenyl; R.sup.2 is selected from the group consisting of H, F, Cl, OMe, and OH; and R.sup.3 is selected from the group consisting of C.sub.4-C.sub.6 cycloalklyl, phenyl and pyridine.

3. The method of claim 1, wherein the compound has the following structure: ##STR00058## wherein R.sup.4 is selected from the group consisting of C.sub.1-C.sub.8 alkyl, C.sub.4-C.sub.6 cycloalklyl, substituted or unsubstituted phenyl, substituted or unsubstituted alkyl amine, substituted or unsubstituted naphthyl and heterocyclic; R.sup.5 is selected from the group consisting of ##STR00059## R.sup.7 is selected from the group consisting of halogen, phenyl, and alkyl; n is 0-5; and Y is selected from the group consisting of H, F, Cl, and -Me.

4. The method of claim 1, wherein the compound has the following structure: ##STR00060## wherein R.sup.3 is selected from the group consisting of C.sub.4-C.sub.6 cycloalkyl, phenyl and pyridine.

5. The method of claim 1, wherein the compounds have the following structures: ##STR00061## wherein R.sup.4 is selected from the group consisting of C.sub.1-C.sub.8 alkyl, C.sub.4-C.sub.6 cycloalklyl, substituted or unsubstituted phenyl substituted or unsubstituted naphthyl, substituted or unsubstituted alkyl amine, and heterocyclic; R.sup.5 is selected from the group consisting of ##STR00062## R.sup.7 is selected from the group consisting of halogen, phenyl, and alkyl; and n is 0-5.

6. The method of claim 5, wherein the compound has the following structure: ##STR00063##

7. The method of claim 1, wherein the parasitic heme auxotrophic organism is Leishmania.

8. The method of claim 7, wherein Leishmania is a promastigote or an amastigote.

9. The method of claim 1, wherein the inhibiting the transport of the heme is lethal to the parasitic heme auxotrophic organism.

10. The method of claim 5, wherein the inhibiting the transport of the heme is lethal to the parasitic heme auxotrophic organism.

11. The method of claim 7, wherein the inhibiting the transport of the heme is lethal to the parasitic heme auxotrophic organism.

12. The method of claim 8, wherein the inhibiting the transport of the heme is lethal to the parasitic heme auxotrophic organism.

13. A kit comprising a compound having the following structure: ##STR00064## wherein R.sup.1 is selected from the group consisting of OH, OMe, and NHR.sup.6, wherein R.sup.6 is selected from the group consisting of C.sub.1-C.sub.5 alkyl, C.sub.3-C.sub.6 cycloalkyl, substituted or unsubstituted pyridine, and phenyl; R.sup.2 is selected from the group consisting of H, F, Cl, OMe, and OH; R.sup.3 is selected from the group consisting of C.sub.4-C.sub.6 cycloalklyl, phenyl, and substituted or unsubstituted pyridine; R.sup.4 is selected from the group consisting of substituted or unsubstituted C.sub.1-C.sub.8 alkyl, C.sub.4-C.sub.6 cycloalklyl, substituted or unsubstituted phenyl, substituted or unsubstituted alkyl amine, naphthyl, and heterocyclic; R.sup.5 is selected from the group consisting of ##STR00065## R.sup.7 is selected from the group consisting of halogen, phenyl, and alkyl; n is 0-5; Y is selected from the group consisting of H, F, Cl, and -Me; and Z is O, or NR.sup.8 wherein R.sup.8 is ##STR00066## and R.sup.9 is selected from the group consisting of OMe, and F.

14. The kit of claim 13, further comprising instructions for using the compounds to inhibit the growth of or kill a parasitic heme auxotrophic organism.

15. The kit of claim 14, wherein the instructions comprise an indication that the parasitic heme auxotrophic organism is Leishmania.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0013] FIG. 1. Compounds identified as LHR1 inhibitors decrease parasite viability. (A) Log phase axenic promastigotes were incubated in basal or heme depleted (HD) M199 media for 48 h (h=hour(s)) in the presence of 50 M of compounds indicated on the x-axis in 1% DMSO (V). (B) Axenic amastigotes were incubated in 5 M or 10 M of the indicated compounds in 1% DMSO for 72 h. Viability (%) was quantitated using Alamar blue compared to DMSO (V).

[0014] FIG. 2. Axenic L. amazonensis amastigotes are sensitive to Cmpd #34 and novel derivatives. Axenic amastigotes were incubated in 5 M or 10 M of the indicated compounds in 1% DMSO (V) for 72 h. Viability was quantified using Alamar Blue.

[0015] FIG. 3. Cmpd #34 and FX40 specifically inhibit LHR1, but not human HRG1, mediated heme uptake in yeast. Yeast cells expressing pGAL1-LHR1 or pGAL1-hHRG1 were incubated in growth medium containing 10 M compounds and GaPPIX for 42 h. Cell growth was measured by absorbance at O.D. 600 and compared to negative control (DMSO, indicated by V), or positive control (0.2% glucose, indicated by Glu). ***p<0.001.

[0016] FIG. 4. Inhibition of intra-macrophage Leishmania amastigotes. Mouse primary BMDMs were first infected with (A) L. amazonensis amastigotes or (B) L. donovani metacyclic promastigotes followed by incubation with the indicated compounds at a concentration of 15 M or DMSO (V), which was used as a negative control, or 0.25 M amphotericin B (AmphoB), which was used as a positive control. Surviving intracellular amastigotes per 100 macrophages were quantified as determined by DAPI positive cells. ****p<0.0001, ***p<0.001, **p<0.005.

[0017] FIG. 5. Dose response curves (IC.sub.50) of FX40 () and the novel derivative compound FX104 (.circle-solid.). (A) BMDMs infected with L. amazonensis amastigotes were treated with various concentrations of the indicated compound for 72 h. BMDMs were lysed and released amastigotes were transformed to promastigotes for 48 h at 26 C. IC.sub.50 values were calculated by quantitation of % promastigote growth using AlamarBlue. (B) Dose response of uninfected BMDMs to compounds. (C) A table showing selectivity criteria for drug efficacy studies. (D) A table showing a summary of high throughput screening.

DETAILED DESCRIPTION

[0018] Unless defined otherwise herein, all technical and scientific terms used in this disclosure have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure pertains.

[0019] Every numerical range given throughout this specification includes its upper and lower values, as well as every narrower numerical range that falls within it, as if such narrower numerical ranges were all expressly written herein.

[0020] The present disclosure provides compounds, pharmaceutical compositions comprising the compounds, and methods of using the compounds for antagonizing one or more pathways required for the transport of heme. This therapeutic strategy was chosen based at least in part on the following observations: (a) heme is utilized in multiple, critical processes; (b) heme is required in all stages of development; (c) Leishmania and other parasitic heme auxotrophs cannot live without heme and actively ingest it; and (d) both humans and their parasites require heme, but the mechanisms used to fulfill this requirement differ between humans and their parasites.

[0021] In general, the disclosure is directed to killing or inhibiting the growth of parasitic organisms that acquire environmental heme, including but not necessarily limited to parasitic helminths, such as ascarids (Ascaris), filarias, hookworms, pinworms (Enterobius) and whipworms (Trichuris trichiura), and members of class Kinetoplastea, including but not necessarily limited to parasites of the genus Trypanosoma, such as T. brucei and T. cruzi and Leishmania, including all members of the Leishmania subgenuses.

[0022] In certain embodiments, the disclosure pertains to inhibiting the transport of heme across a membrane in a parasitic heme auxotrophic organism or in a heme prototroph, such as plasmodium. In embodiments, the disclosure comprises inhibiting the transport of heme across a membrane of a parasitic heme auxotrophic organism. In embodiments the organism that is present in a human or non-human animal. Thus, the disclosure includes human and veterinary therapeutic approaches. In embodiments, the parasitic heme auxotrophic organism is present in an insect or a plant. In embodiments, the parasitic heme auxotrophic organism is present in a cell. In embodiments, the disclosure comprises inhibiting the transport of heme across a membrane of a parasitic heme auxotrophic organism that is present in an insect, such as a fly, or in a mammalian cell, including but not necessarily limited to leukocytes, such as monocytes, neutrophils, macrophage, or hepatocytes. In embodiments, the infected cells are present in an organ in an individual, such as the liver or spleen or bone marrow or skin. In embodiments, the parasitic heme auxotrophic organism is an amastigote or a promastigote.

[0023] In a non-limiting aspect, the present disclosure uses Leishmania as a target parasitic heme auxotrophic organism to demonstrate utility of the compounds described herein. Thus, in embodiments, compounds of the disclosure are selective antagonists of one or more heme transporters in Leishmania. Without intending to be constrained by any particular theory, the heme transporter from Leishmania parasite is used as a non-limiting example of utility of the present compositions and methods according to the following rationale: (a) no satisfactory treatment available except antimonials which are toxic with serious side effects in humans; (b) single-celled protozoa that are genetically manipulatable for ectopic overexpression or gene knockouts; (c) fully sequenced and annotated genome; (d) and growth can be manipulated for pharmacologic assays in free-living (axenic), intracellular (inside macrophages) or in vivo (in mice/hamsters) stages.

[0024] Since previous to the present disclosure no effective drugs exist to treat leishmaniasis except antimonials which are highly toxic to humans, a cell-based, high throughput screen (HTS) of >200,000 compounds using growth assays in yeast was performed. As described below, the disclosure includes identification of several antagonists that specifically inhibit the heme transport function of the Leishmania LHR1 but not the corresponding human homolog (hHRG1). The identified compounds were used in part to design and synthesize the novel compounds described herein.

[0025] In embodiments, the disclosure comprises exposing Leishmania or other parasitic heme auxotrophs to one or more compounds of this disclosure such that is inhibited or they are killed. In certain approaches the method comprises reducing the amount of Leishmania promastigotes, such as in a sandfly, or comprises reducing the amount of Leishmania amastigotes. In one approach the disclosure includes administering to an individual in need thereof a composition comprising one or more of the compounds described herein. This is expected to result in reducing the amount of Leishmania organisms in the individual, or in the eradication of Leishmania organisms from the individual. It is contemplated that the individual can be infected with any Leishmania strain, and can be diagnosed with or suspected of having any type of leishmaniasis, such as cutaneous leishmaniasis, or diffuse cutaneous leishmaniasis, or visceral leishmaniasis. The individual may be a human, or a non-human mammal, including but not necessarily limited to a canine.

[0026] The compounds of this disclosure can be combined with any pharmaceutically acceptable carrier, excipient, diluent and the like to obtain pharmaceutical compositions. Compositions comprising the compounds can be administered to an individual using any suitable route, dosage and formulation. In embodiments, the compositions are administered orally, parenterally, sublingually, transdermally, transmucosally, or topically. Parenteral administration includes, but is not limited to, intravenous, intraarterial, intraperitoneal, subcutaneous, and intramuscular routes. Administration of the compounds can be performed sequentially or concurrently with any other anti-infective compositions of matter and treatment modalities. Further, the disclosure encompasses combinations of the compounds of this disclosure with known anti-infective agents. In embodiments, the compounds of this disclosure can be used in conjunction with anti-infective agents which include but are not necessarily limited to liposomal amphotericin B, pentavalent antimonials, paromomycin, including topical paromomycin, miltefosine, fluconazole, itraconazole, and combinations thereof.

[0027] The disclosure includes pharmaceutically acceptable salts of the compounds disclosed herein.

[0028] The compounds can be provided in any suitable form, including in solutions, suspensions, emulsions, or as dry material for reconstitution prior to use, or in tablets, capsules, and the like. The compounds can be formulated for rapid or sustained release. The compounds can be combined with any suitable drug delivery vehicle, including but not necessarily limited to liposomal formulations, nanoparticle formulations, or other delivery vehicles that will be apparent to those skilled in the art given the benefit of the present disclosure.

[0029] The compounds can be provided as an article of manufacture, such as a kit, that includes, for example, a closed or sealed package that contains the compounds. In certain embodiments, the package can comprise one or more closed or sealed vials, bottles, or any other suitable packaging and/or containers for the sale, or distribution, or use of the compounds. The article of manufacture can include printed information. The printed information can be provided on a label, or on a paper insert, or it can be printed on the packaging material itself. The printed information can include information that identifies the compound, the amounts and types of other active and/or inactive ingredients, and instructions for taking the composition, such as the number of doses to take over a given period of time, and/or information directed to a pharmacist and/or another health care provider, such as a physician, or a patient. In embodiments the printed information includes an indication that the contents of the article of manufacture is for use in treating a parasitic infection, examples of which are described above.

[0030] With respect to the compounds of this disclosure, the following description is pertinent. In particular, as used herein, the term alkyl group, unless otherwise stated, refers to branched or unbranched hydrocarbons. Examples of such alkyl groups include methyl groups, ethyl groups, propyl groups, butyl groups, isopropyl groups, sec-butyl, tert-butyl groups, and the like. For example, the alkyl group is a C.sub.1 to C.sub.8 alkyl group, including all integer numbers of carbons and ranges of numbers of carbons therebetween. Alkyl groups can be substituted with various other functional groups. For example, the alkyl groups can be substituted with groups such as, for example, amines (acyclic and cyclic), alcohol groups, ether groups, and halogen atoms.

[0031] As used herein, the term cycloalkyl group refers to a C.sub.4-C.sub.6 cyclic hydrocarbon group, e.g., cyclopropyl, cyclobutyl, cyclohexyl, and cyclopentyl groups. Cycloalkyl groups can be saturated or partially unsaturated ring systems optionally substituted with, for example, one to three groups, independently selected from the group consisting of alkyl, NH.sub.2, oxo (O), phenyl, naphthyl, haloalkyl (e.g., CF3), halo (e.g., F, Cl, Br, I), alkoxy, and OH groups. Additionally, alkyl substituents can be substituted with various other functional groups.

[0032] As used herein, unless otherwise indicated, halogen means fluorine, chlorine, bromine, and iodine, and halogroup means fluoro, chloro, bromo and iodo.

[0033] As used herein, the term heterocycle or heterocyclic group, unless otherwise stated, refers to a cyclic compound having a ring where at least one or more of the atoms forming the ring is a heteroatom (e.g., oxygen, nitrogen, sulfur, etc.). The heterocyclic ring can be aromatic or nonaromatic, and include compounds that are saturated, partially unsaturated or fully unsaturated. Examples of such groups include azetidine, pyrrolidine, piperdine, azepane, azocane, dihydropyridinone, dihydropyridazinone, dihydrooxepinone, dihydroazepineone, furan, thiophene, oxazole, isoxazole, thiazole, oxadiazole, thiadiazole, triazolo, tetrazole, oxazoline, lactam, lactone, dihydrofuran, tetrahydrofuran, furanone, oxazolone, pyridinone, pyrimidinone, dihydropyridazine, pyranone, oxazinone, and the like. For example, the heterocyclic ring can be a 3, 4, 5, 6, 7, 8, 9, or 10 membered ring containing a number of carbon atoms ranging between 1 and 7 and a number of heteroatoms ranging between 1 and 7. The ring can be bonded to other rings to form ring systems. The heterocyclic ring can be unsubstituted or substituted with various substituents.

[0034] As used herein, unless otherwise indicated, phenyl group means

##STR00007##

where each X is independently a substituent on the phenyl group and m is from 0 to 5. The substituents at different occurrences can be the same or different. For example, the substituents on the phenyl group include substituted or unsubstituted C.sub.1-C.sub.8 alkyl, including all integer numbers of carbons and ranges of numbers of carbons therebetween, substituted or unsubstituted amino, haloalkyl (e.g., CF.sub.3), halo (e.g., F, Cl, Br, I), heterocyclic, substituted or unsubstituted alkoxy (e.g., OMe), and carbonyl containing moieties such as esters and amides. In certain instances, two adjacent R groups can be connected through to form a dioxolyl group.

[0035] As used herein, unless otherwise stated, the term substituents or substituted refers to one or more of the following groups: alkyl groups, amide groups, amino groups, alkoxy groups, aryl groups, cycloalkyl groups, halogen atoms, alkylhalides, heterocyclic groups, hydroxyl groups, ketone, groups, phenyl groups, or a combination thereof.

[0036] The disclosure comprises methods and compounds, where the compounds have one of the following structures:

##STR00008##

where R.sup.1 is selected from the group consisting of OH, OMe, and NHR.sup.6, where R.sup.6 is selected from the group consisting of C.sub.1-C.sub.5 alkyl, C.sub.3-C.sub.6 cycloalkyl, pyridine, and phenyl; R.sup.2 is selected from the group consisting of H, F, Cl, OMe, and OH; R.sup.3 is selected from the group consisting of C.sub.4-C.sub.6 cycloalklyl, phenyl and pyridine; R.sup.4 is selected from the group consisting of C.sub.1-C.sub.8 alkyl, C.sub.4-C.sub.6 cycloalklyl, substituted or unsubstituted phenyl, substituted or unsubstituted alkyl amine, substituted or unsubstituted naphthyl and heterocyclic; R.sup.5 is selected from the group consisting of

##STR00009##

where R.sup.7 is independently selected from the group consisting of halogen atom, phenyl, and alkyl; n equals 0-5, Y is H, F, Cl, or Me; and Z is O or NR.sup.8, where R.sup.8 is

##STR00010##

and R.sup.9 is selected from the group consisting of OMe and F.

[0037] In an embodiment, the compound has the following structures:

##STR00011##

where R.sup.3 is selected from the group consisting of C.sub.4-C.sub.6 cycloalkyl, phenyl, and pyridine.

[0038] In an embodiment, the compound has the following structures:

##STR00012##

where R.sup.4 is selected from the group consisting of C.sub.1-C.sub.8 alkyl, C.sub.4-C.sub.6 cycloalklyl, substituted phenyl, substituted or unsubstituted alkyl amine, naphthyl, substituted naphthyl and heterocyclic; and R.sup.7 is selected from the group consisting of H, halogen, phenyl, and alkyl.

[0039] In an embodiment, the compound has the following structures:

##STR00013##

[0040] Without intending to be bound by any particular theory, a representative scheme is presented herein to describe the synthesis of the aforementioned molecules:

##STR00014##

##STR00015##

##STR00016##

##STR00017##

##STR00018##

[0041] Compounds of this disclosure were designed in part using the following information and approach.

[0042] HRG-1 Heme Transporters and Heme Homeostasis: The identification of HRG-1, the first metazoan heme importer/transporter, was accomplished using C. elegans, a roundworm that does not synthesize its own heme. HRG-1 and its paralog HRG-4 is a four transmembrane domain permease with homologs in vertebrates. Knockdown of hrg1 in zebrafish causes congenital malformations and profound anemia. In mammals, HRG1 is strongly expressed in macrophages of the reticuloendothelial system and specifically localizes to the phagolysosomal membranes during erythrophagocytosis (EP), a process that degrades over 5 million senescent RBCs per second. Depletion of Hrg1 in mouse macrophages causes attenuation of heme transport from the phagolysosomal compartment confirming that the long-sought heme transporter for macrophage heme-iron recycling is the mammalian homolog of C. elegans HRG-1.

[0043] Implications for Host-Pathogen Interactions: Like C. elegans, kinetoplastid parasites such as Trypanosomes and Leishmania cannot synthesize heme as they lack most of the heme synthesizing enzymes. The Leishmania ortholog of worm HRG-4 is designated LHR1, and was the first parasite heme transporter to be identified. Loss of a single LHR1 allele (LHR1.sup.+/) causes severe growth defects while LHR1 null mutants (LHR1.sup./) are not viable which indicates that LHR1-dependent heme transport is essential for parasite survival and that 50% reduction in LHR1 function is sufficient to significantly impair growth.

[0044] A HTS to identify small molecule antagonists of LHR1: To develop a cell-based high throughput screen (HTS) for small molecule antagonists of the LHR1, a heterologous yeast assay was developed. While S. cerevisiae synthesize their own heme (like humans), they lack a dedicated plasma membrane heme transporter. Correspondingly, yeast that are genetically defective in heme synthesis (hem1 mutant) are unable to grow unless they express LHR1 or HRG1 orthologs in the presence of low amounts of supplemented heme in the growth medium. However, if wildtype yeast expressing LHR1 are grown in the presence of a toxic heme analog gallium protoporphyrin IX (GaPPIX) instead of heme in the growth medium, the yeast die because mis-incorporation of GaPPIX into the yeast hemoproteins is cytotoxic as gallium cannot undergo redox reactions like the iron within heme. To exploit this assay, yeast stably-transformed with LHR1 were cultured in 384-well plates and candidate small molecule antagonists of LHR1 were identified from a chemical library of >200,000 compounds on the basis of rescuing yeast growth in the presence of the toxic GaPPIX as measured by optical absorbance for growth using a plate reader. The small molecule library was composed of the following compounds: 75,000 (ChemBridge Corp.); 100,000 (Chemical Diversity Labs); 22,000 (ComGenex); 1200 (TimTek), 1100 (Prestwick); and 450 (NIH clinical collection). The compounds in the library satisfy a relaxed set of Lipinsky's rules with 99% having a molecular weight <550 Da.

[0045] Validation and selectivity of compounds that antagonize LHR1 but not human HRG1: The top 960 hits, defined as being >3 standard deviations from the mean, were cherry-picked for validation and preliminary selectivity assessment (FIG. 5D). From these hits, 373 displayed some activity even at 10-fold lower concentration (0.5 M) with the nine best leads able to fully rescue yeast growth under assay conditions. Though some of these sub-M leads appeared to inhibit GaPPIX uptake in counterscreens of yeast expressing HRG-1's from other species such as C. elegans and human hHRG1, many were selective, displaying little or no human HRG1 antagonism, ruling out the possibility that the hits simply coordinate to GaPPIX. From the 373 compounds that showed selectivity for LHR1 over hHRG1 in the yeast assays, compounds that possessed chemically labile structures, shared similar chemical scaffolds, or showed poor (high) IC.sub.50 values in dose-response curves in yeast were eliminated. This resulted in the identification of 54 most efficacious compounds, all of which were next tested in Leishmania grown in normal medium or in heme depleted medium which upregulates LHR1 (FIG. 1A).

[0046] Validation of compounds in Leishmania: Several of the initial hits are toxic to L. amazonensis promastigotes at 50 M, with enhanced toxicity observed in wildtype strains grown in heme-deficient media that upregulates LHR1, supporting the concept that the drug effects are mediated, in part, through the LHR1 transporter itself (FIG. 1A). Although some of the HTS hits appear to have metabolic and chemical liabilities, several chemical series represent potent and drug-like inhibitors. Strikingly, some of the compounds decreased L. amazonensis viability in axenic culture by >90% at 5 M and 10 M such as Cmpd #34, a 6,7,8,9-tetrahydro-2-hydroxy-1-dibenzofuranyl derivative. This compound was not identified from any other HTS that is publicly available (FIG. 1A&B).

[0047] To validate the in vivo efficacy of lead compounds against intracellular amastigotes in macrophages, primary bone marrow-derived macrophages (BMDMs) from mice were obtained and infected them with L. amazonensis amastigotes followed by incubation for 72 h with LHR1 antagonists or amphotericin B which kills intracellular Leishmania, as a positive control. Cells were then fixed and stained with DAPI which permits visualization of the host macrophage nuclei and parasite nuclei and kinetoplasts. Images collected with fluorescent microscopy were used to determine the ratio between total number of parasites and total number of host cells per treatment. Toxicity to host cells were measured by AlamarBlue viability assays. Both Cmpd #34 and novel antagonist FX40 killed about 50% of intracellular L. amazonensis amastigotes (FIG. 4A). The demonstration of this property of FX40 led to the development of FX104. (Sch. 1 and FIG. 4A) The structure of FX104 is shown in Scheme 1. Without intending to be constrained by any particular theory or approach, FX104 was determined to have the best drug-like parameters with significantly lower LogP value; better solubility, absorption and metabolic stability; and decreased P-gp efflux. The results show that (a) the novel compound FX104, synthesized from the original scaffold of FX40, is over 30-fold more effective against intracellular forms of Leishmania (IC.sub.50: 8 M versus 250 nM, FIG. 5A); and (b) FX104 has little or no toxicity in BMDMs even at 40-fold greater concentrations (FIG. 5B).

[0048] L. donovani metacyclic promastigotes were selectively enriched using a Ficoll density-step gradient. L. donovani metacyclic promastigotes were incubated with BMDMs at a macrophage:promastigote ratio of 1:10. After a 24 h incubation at 37 C. in 5% CO.sub.2, non-internalized promastigotes were removed by 4 washes and test compounds were added to the cultures and incubated at 37 C. for 72 h. Intracellular parasite viability was evaluated by DAPI staining. Consistent results obtained with L. amazonensis, Cmpd #34, FX40 and FX104 killed between 50-70% of intracellular parasites (FIG. 4B). As shown in FIG. 5C, embodiments of the disclosure include iterative SAR and CSP modeling to improve the efficacy of compounds of this disclosure in intra-macrophage assays, which may be performed prior to evaluating them for metabolic stability and ADME properties. In embodiments, compounds that attain an IC.sub.50 value that matches or surpasses that for Amphotericin B: IC.sub.50=0.05-0.1 M against intra-macrophage L. amazonensis or 0.02-0.06 M for intra-macrophage L. donovani (>90% efficacy) are provided.

[0049] To further assess the chemical stability of compounds of this disclosure, the compounds can be incubated with simulated gastric fluid, simulated intestinal fluid, mouse plasma, mouse liver S9 fraction as well as Caco-2 cell homogenate, and assessed as follows. After incubation for varying times, samples are removed and subsequently ice-cold acetonitrile is added to precipitate cellular protein and stop enzymatic activity. The samples are centrifuged at 12,000 g at 4 C. for 30 min and supernatant are collected for LC-MS/MS analysis. To determine the enzymes responsible for the metabolism of the compounds, mouse or human liver microsomes are prepared by differential centrifugation. The test compounds are incubated with microsomal preparation for varying times, along with representative cytochrome P450 (CYP) inhibitors and antibodies. The metabolic reactions are stopped and the extracted samples are subjected to LC-MS/MS analysis for metabolite identification, quantitation, and metabolism kinetics. For toxicity evaluation in cells, the effect of lead compounds on the viability (MTT assay), cellular morphology (microscopy and histology), and nucleic acid stability (DNA fragmentation) can be evaluated, for example, in HeLa, HepG2, and/or HEK293 cells.

[0050] The following materials and methods were employed to synthesize compounds of this disclosure as described in the Examples.

Synthesis Materials and Methods

[0051] ##STR00019##

[0052] Step 1: To a solution of compound 1 in toluene was added Ar(R5)-H followed by AlCl3. The reaction mixture was allowed to stir for 24 h, and then concentrated. After removing the solvent, the crude material was purified by flash column chromatograph to get compound 2.

[0053] Step 2: To a solution of compound 2 in dichloromethane was added SOCl2. The reaction was heated under reflux for 2 h. After removing the solvent, the resulting material was redissolved in DMF, to the resulting solution was added the amino compound R4NH2, followed by triethylamine. The reaction mixture was allowed to stir at room temperature overnight, and then concentrated. After removing the solvent, the crude material was purified by flash column chromatograph to get the final compounds. Examples of compounds encompassed by this disclosure include but are not limited to the following:

EXAMPLE 1

[0054] The following is an example of synthesis and characterization of a compound of the present disclosure. Methyl 2-(4-fluoro-1,3-dihydro-3-(naphthalen-2-yl)-1-oxoisobenzofuran-3-ylamino)benzoate

##STR00020##

[0055] Yield 31.0%. .sup.1H NMR (400 MHz, CDCl.sub.3) (ppm) 9.51 (s, 1H), 8.12 (s, 1H), 7.96-7.94 (d, J=7.6 Hz, 1H), 7.82-7.77 (m, 4H), 7.71-7.73 (d, J=8.4, 1H), 7.58-7.52 (m, 1H), 7.48-7.46 (m, 2H), 7.32-7.28 (t, J=8.4 Hz, 1H), 7.08-7.04 (t, J=8.0 Hz, 1H), 6.79-6.76 (d, J=8.4 Hz, 1H), 6.74-6.70 (t, J=8.0 Hz, 1H), 3.90 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) (ppm) 164.3, 163.4, 52.8, 150.3, 141.4, 132.0, 130.0, 129.2, 128.6, 128.4, 128.2, 128.1, 126.6, 124.3, 123.8, 122.8, 122.1, 121.8, 121.0, 118.8, 117.8, 117.6, 117.4, 113.6, 112.5, 108.3, 90.7, 47.3. ESI-MS m/z 426.1 [MH].sup..

EXAMPLE 2

[0056] The following is an example of synthesis and characterization of a compound of the present disclosure. Methyl 2-(4-fluoro-1,3-dihydro-3-(3,4-dimethylphenyl)-1-oxoisobenzofuran-3-ylamino) benzoate

##STR00021##

[0057] Yield 31.8%. .sup.1H NMR (400 MHz, CDCl.sub.3) (ppm) 9.37 (s, 1H), 7.95-7.93 (d, J=7.2 Hz, 1H), 7.75-7.73 (d, J=7.6 Hz, 1H), 7.56-7.50 (m, 1H), 7.37-7.27 (m, 3H), 7.15-7.11 (t, J=7.6 Hz, 1H), 7.09-7.07 (d, J=7.6 Hz, 1H),6.75-6.71 (m, 2H), 3.87 (s, 1H), 2.20 (s, 6H). .sup.13C NMR (100 MHz, CDCl.sub.3) (ppm) 169.0, 168.4, 155.2, 146.2, 137.9, 137.6, 134.9, 134.0, 132.8, 131.5, 130.5, 128.7, 127.2, 123.9, 122.7, 122.5, 122.2, 118.4, 117.4, 51.9, 19.9, 19.4. ESI-MS m/z 404.1 [MH].sup..

EXAMPLE 3

[0058] The following is an example of synthesis and characterization of a compound of the present disclosure. Methyl 2-(4-fluoro-1,3-dihydro-1-oxo-3-p-tolylisobenzofuran-3-ylamino)benzoate

##STR00022##

[0059] Yield 32.6%. .sup.1H NMR (400 MHz, CDCl.sub.3) (ppm) 9.40 (s, 1H), 7.97-7.95 (d, J=8.0 Hz, 1H), 7.77-7.75 (d, J=6.8. 1H), 7.58-7.53 (m, 1H), 7.53-7.50 (d, J=7.6 Hz, 1H), 7.37.34-7.32 (t, J=7.6 Hz, 1H), 7.17-7.13 (m, 3H), 6.75-6.72 (m, 2H), 3.89 (s, 3H), 2.32 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) (ppm) 169.1, 168.2, 157.6, 155.12, 146.3, 139.1, 136.8, 134.4, 133.9, 132.8, 131.4, 129.8, 128.6, 126.2, 122.5, 122.3, 122.1, 118.3, 117.3, 113.1, 95.6, 52.0, 21.2. ESI-MS m/z 390.1 [MH].sup..

EXAMPLE 4

[0060] The following is an example of synthesis and characterization of a compound of the present disclosure. 2-(4-fluoro-1,3-dihydro-3-(naphthalen-2-yl)-1-oxoisobenzofuran-3-ylamino)-N-methylbenzamide

##STR00023##

[0061] Yield 39.9%. .sup.1H NMR (400 MHz, CDCl.sub.3) (ppm) 9.46 (s, 1H), 8.06 (s, 1H), 7.75-7.67 (m, 5H), 7.48-7.43 (m, 1H), 7.40-7.38 (m, 2H), 7.30-7.28 (d, J=7.6 Hz, 1H), 7.22-7.20 (m, 1H), 6.95-6.91 (t, J=8.0 Hz, 1H), 6.73-6.71 (d, J=8.8 Hz, 1H), 6.66-6.62 (t, J 6.8 Hz, 1H), 6.12 (br s, 1H), 2.92-2.91 (d, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) (ppm) 169.0, 168.4, 155.2, 144.2, 135.0, 133.2, 132.6, 132.0, 128.9, 128.6, 127.6, 126.9, 126.4, 123.7, 122.2, 118.4, 29.7, 26.8. ESI-MS m/z 425.1 [MH].sup..

EXAMPLE 5

[0062] The following is an example of synthesis and characterization of a compound of the present disclosure. Methyl 2-(1,3-dihydro-1-oxo-3-p-tolylisobenzofuran-3-ylamino)benzoate

##STR00024##

[0063] Yield 39.9%. .sup.1H NMR (CDCl.sub.3) (ppm) 9.20 (br s, 1H), 7.96-7.92 (t, J=8.8 Hz, 2H), 7.68-7.64 (t, J=7.6 Hz, 1H), 7.57-7.53 (m, 2H), 7.50-7.48 (d, J=7.6 Hz, 2H), 7.16-7.14 (m, 3H), 6.83-6.81 (d, J=8.8 Hz, 1H), 6.76-6.72 (t, J=7.6 Hz, 1H), 3.87 (s, 3H), 2.30 (s, 3H). .sup.13C NMR (CDCl.sub.3) (ppm) 169.5, 169.0, 151.5, 146.5, 138.8, 135.9, 135.3, 133.9, 131.2, 130.2, 130.0, 126.0, 125.5, 125.1, 122.1, 118.2, 117.5, 112.9, 91.5, 51.9, 21.1. ESI-MS m/z 372.1[MH].sup..

EXAMPLE 6

[0064] The following is an example of synthesis and characterization of a compound of the present disclosure. Ethyl 3-(1,3-dihydro-1-oxo-3-p-tolylisobenzofuran-3-ylamino)benzoate

##STR00025##

[0065] Yield 38.2%. .sup.1H NMR (400 MHz, CDCl.sub.3) (ppm) 7.91-7.89 (d, J=7.6 Hz, 1H), 7.67-7.63 (t, J=7.6 Hz, 1H), 7.57-7.53 (m, 4H), 7.51-7.49 (d, J=7.2Hz, 1H), 7.38 (s, 1H), 7.18-7.16 (d, J=8.4 Hz, 2H), 7.13-7.09 (t, J=7.6 Hz, 1H), 6.88-6.86 (d, J=8.0Hz, 1H), 4.29-4.24 (m, 2H), 2.32 (s, 3H), 1.33-1.29 (t, J=7.2 Hz, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) (ppm) 169.2, 166.3, 150.7, 142.5, 139.0, 135.8, 134.8, 131.1, 130.3, 129.9, 128.8, 126.0, 125.6, 122.8, 122.4, 122.0, 119.3, 60.8, 21.1, 14.2. ESI-MS m/z 386.1 [MH].sup..

EXAMPLE 7

[0066] The following is an example of synthesis and characterization of a compound of the present disclosure. 3-(4-methoxybenzylamino)-3-p-tolylisobenzofuran-1 (3H)-one

##STR00026##

[0067] Yield 24.3%. .sup.1H NMR (400 MHz, CDCl.sub.3) (ppm) 7.63-7.62 (d, J=7.6 Hz, 1H), 7.44-7.40 (t, J=7.2 Hz, 1H), 7.38-7.34 (t, J=7.2 Hz, 1H), 7.25-7.20 (m, 2H), 7.07-7.05 (m. 4H), 6.57-6.54 (d, J=8.4 Hz, 2H), 4.49-4.46 (d, J=14.8 Hz, 1H), 4.03-4.00 (d, J=14.8 Hz, 1H), 3.62 (s, 3H), 2.32 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) (ppm) 167.9, 158.2, 149.4, 138.0, 135.7, 132.6, 130.3, 130.2, 129.2, 129.0, 126.3, 123.2, 122.6, 113.3, 61.7, 55.1, 42.4, 21.1. ESI-MS m/z 358.2 [MH].sup..

EXAMPLE 8

[0068] The following is an example of synthesis and characterization of a compound of the present disclosure. 3-(4-chlorophenylamino)-3-p-tolylisobenzofuran-1 (3H)-one

##STR00027##

[0069] Yield 37.4%. .sup.1H NMR (400 MHz, CDCl.sub.3) (ppm) 7.89-7.87 (d, J=8.0 Hz, 1H), 7.65-7.62 (t, J=6.8 Hz, 1H), 7.55-7.52 (m, 2H), 7.17-7.15 (d, J=7.6 Hz, 2H), 7.04-7.01 (d, J=7.6 Hz, 2H), 6.66-6.64 (d, J=8.4 Hz, 2H), 2.32 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) (ppm) 169.2, 14.09, 139.2, 135.5, 134.8, 130.4, 129.9, 128.8, 126.1, 125.7, 122.8, 120.0, 21.1. ESI-MS m/z 348.1 [MH].sup..

EXAMPLE 9

[0070] The following is an example of synthesis and characterization of a compound of the present disclosure. 3-(pentylamino)-3-p-tolylisobenzofuran-1 (3H)-one

##STR00028##

[0071] Yield 55.4%. .sup.1HNMR (400 MHz, CDCl.sub.3) (ppm) 7.70-7.68 (d, J=7.2 Hz, 1H), 7.47-7.39 (m, 2H), 7.26-7.25 (m, 3H), 7.14-7.12 (d, J=8.0 Hz, 2H), 3.48 (br s, 1H), 3.44-3.37 (m, 1H), 2.96-2.89 (m, 1H), 2.33 (s, 3H), 1.51-1.45 (m, 1H), 1.38-1.34 (m, 1H), 1.23-1.14 (m, 4H), 0.82-0.78 (t, J=6.8 Hz, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) (ppm) 167.7, 149.1, 138.2, 135.6, 132.4, 130.6, 129.4, 129.1, 126.1, 123.1, 122.5, 91.4, 39.6, 29.3, 28.4, 22.2, 21.1, 13.9. ESI-MS m/z 308.2 [MH].sup..

EXAMPLE 10

[0072] The following is an example of synthesis and characterization of a compound of the present disclosure. 3-(3,4-dimethylphenyl)-3-(propylamino)isobenzofuran-1 (3H)-one

##STR00029##

[0073] Yield 24.7%. .sup.1H NMR (400 MHz, CDCl.sub.3) (ppm) 7.59-7.57 (d, J=7.2 Hz, 1H), 7.45-7.42 (t, J=8.0 Hz, 1H), 7.38-7.34 (t, J=8.0 Hz, 1H), 7.27-7.25 (d, J=7.2 Hz, 1H), 7.13-7.07 (m, 3H), 4.12 (s, 1H), 3.37-3.30 (m, 1H), 2.90-2.82 (m, 1H), 2.24 (s, 3H), 2.22 (s, 3H), 1.51-1.47 (m, 1H), 1.40-1.34 (m, 1H), 0.79-0.75 (t, J=7.2 Hz, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) (ppm) 167.9, 149.3, 136.8, 136.7, 136.0, 132.4, 130.4, 129.7, 129.1, 127.1, 123.6, 123.1, 122.5, 91.5, 41.3, 22.0, 19.9, 19.5. 11.7. ESI-MS m/z 294.1 [MH].sup..

EXAMPLE 11

[0074] The following is an example of synthesis and characterization of a compound of the present disclosure. 3-(isopropylamino)-3-(3,4-dimethylphenyl)isobenzofuran-1 (3H)-one

##STR00030##

[0075] Yield 19.6%. .sup.1H NMR (400 MHz, CDCl.sub.3) (ppm) 7.68-7.66 (m, 1H), 7.41-7.38 (m, 2H), 7.20-7.14 (m, 3H), 7.09-7.07 (d, J=8.0 Hz, 1H), 3.60-3.55 (m, 1H), 3.47 (br s, 1H), 2.24 (s, 3H), 2.22 (s, 3H), 1.42-1.41 (d, J=6.8 Hz, 3H), 1.26-1.24 (d, J=6.8 Hz, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) 6 (ppm) 167.4, 148.8, 136.7, 136.5, 136.0, 132.2, 131.6, 130.9, 130.1&129.9, 129.7&129.5, 129.3, 128.2&128.0, 127.7, 123.8, 122.9, 122.4, 91.9, 44.8, 22.3, 21.2, 19.8, 19.5. ESI-MS m/z 318.1 [M+Na].sup.+.

EXAMPLE 12

[0076] The following is an example of synthesis and characterization of a compound of the present disclosure. 3-(2-(dimethylamino)ethylamino)-3-(3,4-dimethylphenyl)isobenzofuran-1 (3H)-one

##STR00031##

[0077] Yield 23.6%. .sup.1H NMR (400 MHz, CDCl.sub.3) (ppm) 7.81-7.80 (d, J=6.8 Hz, 1H), 7.44-7.38 (m, 2H), 7.22-7.19 (m, 2H), 7.11-7.09 (m, 2H), 4.14-4.11 (d, J=14.0 Hz, 1H), 2.82-2.73 (m, 2H), 2.28-2.18 (m, 14H). .sup.13C NMR (100 MHz, CDCl.sub.3) (ppm) 168.2, 150.1, 137.8, 136.8, 136.6, 132.6, 129.9, 128.6, 127.4, 126.3, 123.9, 123.3, 122.9, 122.2, 58.8, 44.6, 37.0, 19.9, 19.4. ESI-MS m/z 323.2 [MH].sup..

EXAMPLE 13

[0078] The following is an example of synthesis and characterization of a compound of the present disclosure. 3-(3-chlorophenylamino)-3-(3,4-dimethylphenyl)isobenzofuran-1 (3H)-one

##STR00032##

[0079] Yield 9.70%. .sup.1H NMR (400 MHz, CDCl.sub.3) (ppm) 7.94-7.93 (d, J=6.8 Hz, 1H), 7.69-7.65 (t, J 6.8 Hz, 1H), 7.59-7.54 (m, 2H), 7.42-7.40 (m, 2H), 7.15-7.13 (d, J=7.6 Hz, 1H), 7.02-6.98 (t, J=8.0 Hz, 1H), 6.83-6.82 (d, J=7.6 Hz, 1H), 6.73 (s, 1H), 6.59-6.57 (d, J=7.2 Hz, 1H), 2.25 (s, 6H). .sup.13C NMR (100 MHz, CDCl.sub.3) (ppm) 169.2, 143.6, 137.7, 134.8, 134.4, 130.5, 130.4, 129.8, 126.5, 126.1, 123.0, 122.7, 121.0, 118.3, 116.3, 20.0, 19.5. ESI-MS m/z 386.0 [M+Na].sup.+.

EXAMPLE 14

[0080] The following is an example of synthesis and characterization of a compound of the present disclosure. 3-(2-bromophenylamino)-3-(3,4-dimethylphenyl)isobenzofuran-1 (3H)-one

##STR00033##

[0081] Yield 9.70%. .sup.1H NMR (400 MHz, CDCl.sub.3) (ppm) 7.94-7.92 (d, J=6.8 Hz, 1H), 7.68-7.64 (t, J=7.6 Hz, 1H), 7.58-7.54 (m, 2H), 7.46-7.44 (d, J=8.0 Hz, 1H), 7.38-7.36 (m, 2H), 7.12-7.10 (d, J=8.4 Hz, 1H), 6.96-6.93 (t, J=7.2 Hz, 1H), 6.76-6.74 (d, J=6.8 Hz, 1H), 6.70-6.67 (t, J=7.6 Hz, 1H), 2.22 (s, 3H), 2.21 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) (ppm) 169.3, 150.9, 140.0, 137.7, 135.8, 135.0, 132.3, 130.5, 130.4, 127.9, 126.5, 126.1, 125.5, 123.0, 122.5, 121.4, 118.5, 112.4, 20.0, 19.5. ESI-MS m/z 406.1, 408.1 [MH].sup.

EXAMPLE 15

[0082] The following is an example of synthesis and characterization of a compound of the present disclosure. 3-(4-bromo-2-methoxyphenylamino)-3-(3,4-dimethylphenyl)isobenzofuran-1 (3H)-one

##STR00034##

[0083] Yield 11.1%. .sup.1H NMR (400 MHz, CDCl.sub.3) (ppm) 7.92-7.90 (d, J=8.0 Hz, 1H), 7.66-7.63 (t, J=8.0 Hz, 1H), 7.57-7.53 (m, 2H), 7.39-7.37 (m, 2H), 7.13-7.11 (d, J=8.4 Hz, 1H), 6.90-6.89 (d, J=2.4 Hz, 1H), 6.73-6.70 (dd, J1=8.8 Hz, J2=1.6 Hz, 1H), 6.37-6.34 (d, J=8.8 Hz, 1H), 3.83 (s, 3H), 2.23 (s, 6H). .sup.13C NMR (100 MHz, CDCl.sub.3) (ppm) 169.4, 151.0, 148.6, 137.7, 136.2, 134.8, 131.4, 130.5, 130.2, 126.5, 126.0, 125.6, 124.0, 123.8, 123.4, 123.0, 122.7, 117.5, 113.3, 112.0, 55.8, 20.0, 19.5. ESI-MS m/z 460.0, 462.0 [M+Na].sup.+.

EXAMPLE 16

[0084] The following is an example of synthesis and characterization of a compound of the present disclosure. 3-(benzylamino)-3-(3,4-dimethylphenyl)isobenzofuran-1 (3H)-one

##STR00035##

[0085] Yield 20.6%. .sup.1H NMR (400 MHz, CDCl.sub.3) (ppm) 7.71-7.69 (d, J=3.6 Hz, 1H), 7.44-7.36 (m, 2H), 7.25-7.23 (m, 1H), 7.15-7.08 (m, 5H), 7.03-7.01 (d, J=8.4 Hz, 1H), 6.91 (s, 1H), 4.59-4.56 (d, J=15.6 Hz, 1H), 4.08-4.04 (d, J=15.6 Hz, 1H), 2.19 (s, 3H), 2.09 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) (ppm) 168.0, 149.2, 138.1, 136.8, 136.6, 135.5, 132.7, 130.1, 129.6, 129.3, 128.8, 128.0, 127.5, 126.9, 123.7, 123.3, 122.7, 91.7, 43.0, 19.8, 19.4. ESI-MS m/z 342.2 [MH].sup..

EXAMPLE 17

[0086] The following is an example of synthesis and characterization of a compound of the present disclosure. Methyl 2-(1,3-dihydro-1-(3,4-dimethylphenyl)-3-oxoisobenzofuran-1-ylamino)benzoate

##STR00036##

[0087] Yield 12.8%. .sup.1H NMR (400 MHz, CDCl.sub.3) (ppm) 9.16 (br s, 1H), 8.00-7.91 (t, J=8.0 Hz, 2H), 7.68-7.64 (t, J=7.6 Hz, 1H), 7.57-7.53 (m, 2H), 7.34-7.33 (m, 2H), 7.18-7.16 (m, 1H), 7.10-7.08 (d, J 8.8 Hz, 1H), 6.83-6.81 (d, J 8.8 Hz, 1H), 6.75-6.72 (t, J=7.6 Hz, 1H), 3.87 (s, 3H), 2.21 (s, 6H). .sup.13C NMR (100 MHz, CDCl.sub.3) (ppm) 169.6, 169.0, 151.7, 146.6, 137.7, 137.4, 136.2, 135.2, 133.9, 131.2, 130.5, 130.2, 126.5, 126.0, 125.1, 122.9, 122.1, 118.1, 117.5, 112.8, 91.7, 51.9, 20.0, 19.4. ESI-MS m/z 388.1 [M+H].sup.+.

EXAMPLE 18

[0088] The following is an example of synthesis and characterization of a compound of the present disclosure. Methyl-2-(4-chloro-1,3-dihydro-3-(3,4-dimethylphenyl)-1-oxoisobenzofuran-3-ylamino) benzoate

##STR00037##

[0089] Yield 17.9%. .sup.1H NMR (400 MHz, CDCl.sub.3) (ppm) 9.44 (br s, 1H), 7.98-7.96 (d, J 7.2 Hz, 1H), 7.91-7.89 (d, J 7.6 Hz, 1H), 7.60-7.58 (d, J 7.2 Hz, 1H), 7.54-7.51 (t, J=8.0 Hz, 1H), 7.34-7.32 (d, J=7.6 Hz, 1H), 7.24 (s, 1H), 7.16-7.12 (t, J=8.0 Hz, 1H), 7.10-7.08 (d, J=8.8 Hz, 1H), 6.77-6.73 (t, J=8.0 Hz, 1H), 6.70-6.68 (d, J=8.4 Hz, 1H), 3.89 (s, 3H), 2.23 (s, 3H), 2.20 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) (ppm) 169.0, 168.2, 146.6, 146.3, 137.8, 137.1, 136.3, 133.8, 132.9, 131.8, 131.3, 129.7, 129.3, 128.7, 127.9, 125.0, 124.6, 118.1, 117.2, 112.9, 91.9, 51.9, 20.0, 19.5. ESI-MS m/z 422.0 [M+H].sup.+.

EXAMPLE 19

[0090] The following is an example of synthesis and characterization of a compound of the present disclosure. 3-(2-methoxyphenylamino)-4-chloro-3-p-tolylisobenzofuran-1 (3H)-one

##STR00038##

[0091] Yield 19.7%. .sup.1H NMR (400 MHz, CDCl.sub.3) (ppm) 7.89-7.87 (d, J=7.2 Hz, 1H), 7.58-7.50 (m, 2H), 7.39-7.37 (d, J=7.6 Hz, 1H), 7.34 (s, 1H), 7.13-7.11 (d, J=8.0 Hz, 1H), 6.84-6.76 (m, 2H), 6.61-6.57 (t, J=8.0 Hz, 1H), 6.39-6.37 (d, J=7.6 Hz, 1H), 3.87 (s, 3H), 2.25 (s, 3H), 2.24 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) (ppm) 168.1, 148.2, 146.1, 137.9, 137.0, 135.8, 134.0, 131.9, 131.7, 129.7, 129.5, 127.9, 124.7, 124.5, 120.6, 120.2, 116.2, 110.1, 55.7, 20.0, 19.5. ESI-MS m/z 394.1 [M+H].sup.+.

EXAMPLE 20

[0092] The following is an example of synthesis and characterization of a compound of the present disclosure. 3-(2-fluorophenylamino)-4-chloro-3-(3,4-dimethylphenyl)isobenzofuran-1 (3H)-one

##STR00039##

[0093] Yield 29.8%. .sup.1H NMR (400 MHz, CDCl.sub.3) (ppm) 7.90-7.88 (d, J=7.6 Hz, 1H), 7.59-7.51 (m, 2H), 7.38-7.34 (m, 2H), 7.14-7.12 (d, J=8.0 Hz, 1H), 7.05-6.99 (m, 1H), 6.79-6.75 (m, 2H), 6.55-6.51 (m, 1H), 2.25 (s, 3H), 2.24 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) (ppm) 167.8, 154.1, 151.7, 145.5, 138.2, 137.2, 136.0, 133.3, 132.0, 130.7&130.6, 129.8, 129.3, 124.6&124.6 (1C), 124.0&124.0 (1C), 121.0&120.9 (1C), 115.0, 114.8, 20.0, 19.5. ESI-MS m/z 382.0 [M+H].sup.+.

EXAMPLE 21

[0094] The following is an example of synthesis and characterization of a compound of the present disclosure. 3-(4-(4-chlorophenoxy)phenylamino)-4-chloro-3- (3,4-dimethylphenyl)isobenzofuran-1 (3H)-one

##STR00040##

[0095] Yield 18.2%. .sup.1H NMR (400 MHz, CDCl.sub.3) (ppm) 7.77-7.75 (d, J=6.8 Hz, 1H), 7.51-7.44 (m, 2H), 7.28-7.2 (m, 3H), 7.19-7.16 (d, J=8.8 Hz, 2H), 7.04-6.99 (m, 3H), 6.92-6.89 (d, J=8.8 Hz, 2H), 6.85-6.82 (d, J=8.4 Hz, 2H), 2.20 (s, 3H), 2.15 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) (ppm) 165.9, 155.8, 144.0, 137.1, 136.4, 134.2, 133.9, 133.0, 131.3, 130.1, 129.7, 129.4, 128.8, 127.5, 123.9, 122.3, 120.4, 118.6, 19.9, 19.5. ESI-MS m/z 513.0 [M+Na].sup.+.

EXAMPLE 22

[0096] The following is an example of synthesis and characterization of a compound of the present disclosure. 3,4-bis (2-methoxyphenylamino)-3-p-tolylisobenzofuran-1 (3H)-one

##STR00041##

[0097] Yield 10.7%. .sup.1H NMR (Acetone-d6) (ppm) 7.79-7.77 (d, J=7.2 Hz, 1H), 7.70-7.67 (m, 1H), 7.33-7.29 (t, J=8.4 Hz, 2H), 7.10-7.00 (m, 5H), 6.92-6.84 (m, 3H), 6.72-6.69 (t, J=8.0 Hz, 1H), 6.60-6.56 (t, J=8.0 Hz, 1H), 6.40-6.38 (d, J=8.0 Hz, 1H). 5.62 (br s, 1H), 3.85 (s, 3H), 3.17 (s, 3H), 2.29 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) (ppm) 165.3, 158.3, 156.3, 155.8, 148.0, 138.6, 136.3, 135.9, 134.1, 132.2, 131.6&131.5, 130.6, 129.7, 128.7, 125.6, 123.1, 121.0, 120.4, 120.0, 119.7, 119.5, 119.0, 113.8, 111.7, 109.8, 81.9, 55.8, 55.2, 21.0. ESI-MS m/z 491.1 [M+Na].sup.+.

EXAMPLE 23

[0098] The following is an example of synthesis and characterization of a compound of the present disclosure. 3-(2-methoxyphenylamino)-4-fluoro-3-p-tolylisobenzofuran-1 (3H)-one

##STR00042##

[0099] Yield 31.5%. .sup.iH NMR (400 MHz, CDCl.sub.3) (ppm) 7.76-7.74 (d, J=7.6 Hz, 1H), 7.57-7.53 (m, 1H), 7.32-7.28 (m, 1H), 7.25-7.20 (m, 3H), 7.09-7.07 (d, J=8.0 Hz, 1H), 7.00-6.98 (d, J=8.0 Hz, 1H), 6.85-6.81 (t, J=7.6 Hz, 1H), 6.71-6.69 (d, J=7.6 Hz, 1H), 3.79 (s, 3H), 2.31 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) (ppm) 158.3, 156.0, 155.7, 131.9&131.9, 131.5, 130.1, 129.5&129.4, 18.8, 126.0, 121.3, 120.5, 120.3, 119.8&119.8, 56.1, 21.2. ESI-MS m/z 386.0 [M+Na].sup.+.

EXAMPLE 24

[0100] The following is an example of synthesis and characterization of a compound of the present disclosure. 3,4-bis (2-fluorophenylamino)-3-p-tolylisobenzofuran-1 (3H)-one

##STR00043##

[0101] Yield 14.1%. .sup.1H NMR (400 MHz, CDCl.sub.3) (ppm) 7.93-7.91 (d, J=7.6 Hz, 1H), 7.62-7.58 (m, 1H), 7.36-7.31 (m, 1H), 7.24-7.20 (m, 2H), 7.17-7.10 (m, 5H), 7.08-7.03 (m,2H), 6.80-6.76 (m, 2H), 6.60-6.54 (m, 1H), 5.25-5.24 (d, J=4.8 Hz, 1H), 2.36 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) (ppm) 165.1, 160.3, 158.2, 157.8, 155.6, 153.8, 151.4, 139.4, 135.4, 134.7, 132.3, 132.1, 132.1, 130.1, 130.0&130.0, 129.5, 125.1, 124.5&124.5, 124.3&124.3, 122.2&122.1, 120.3, 120.1&120.1, 116.5&116.3, 115.8, 114.8&114.7, 81.9, 21.1. ESI-MS m/z 467.1 [M+Na].sup.+.

EXAMPLE 25

[0102] The following is an example of synthesis and characterization of a compound of the present disclosure. 3-((pyridin-2-yl)methylamino)-4-fluoro-3-p-tolylisobenzofuran-1 (3H)-one

##STR00044##

[0103] Yield 82.9%. .sup.1H NMR (400 MHz, CDCl.sub.3) (ppm) 9.20 (br s, 1H), 8.41-8.39 (d, J=4.4 Hz, 1H), 7.69-7.65 (td, J1=8.0 Hz, J2=1.6 Hz, 1H), 7.59-7.57 (d, J=7.6 Hz, 1H), 7.42-7.38 (m, 4H), 7.21-7.11 (m, 4H), 5.01-4.97 (d, J=16.4 Hz, 1H), 4.16-4.12 (d, J=16.4 Hz, 1H), 2.32 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) (ppm) 166.9, 158.2, 156.1, 155.7, 148.0, 138.4, 138.0, 136 . . . 2, 132.6, 131.3 & 131.2, 129.3, 126.3, 122.8&122.8, 120.3, 120.1, 119.2, 89.5, 44.6, 21.1. ESI-MS m/z 371.1 [M+Na].sup.+.

EXAMPLE 26

[0104] The following is an example of synthesis and characterization of a compound of the present disclosure. 4-fluoro-3-(3,4-dimethylphenyl)-3-(pyridin-2-ylamino)isobenzofuran-1 (3H)-one

##STR00045##

[0105] Yield 5.52%. .sup.1H NMR (400 MHz, CDCl.sub.3) (ppm) 8.42-8.40 (d, J=7.6 Hz, 1H), 8.15-8.14 (d, J=4.0 Hz, 1H), 7.78-7.75 (m, 2H), 7.50-7.45 (m, 1H), 7.28-7.24 (m, 2H), 7.21-7.17 (t, J=8.4 Hz, 1H), 7.02-6.98 (m, 2H), 2.16 (s, 6H). .sup.13C NMR (100 MHz, CDCl.sub.3) ppm) 165.9, 158.0&155.5 (1C), 151.4, 146.5, 138.6, 137.5, 136.6&136.6 (1C), 136.6&131.6 (1C), 129.6, 126.9, 123.2, 121.2 &121.1 (1C), 120.0, 120.0, 116.5, 93.2, 19.9, 19.4. ESI-MS m/z 371.0 [M+Na].sup.+.

EXAMPLE 27

[0106] The following is an example of synthesis and characterization of a compound of the present disclosure. 3,4-bis (2-fluorophenylamino)-3-(3,4-dimethylphenyl)isobenzofuran-1 (3H)-one

##STR00046##

[0107] Yield 31.0%. .sup.1H NMR (400 MHz, CDCl.sub.3) (ppm) 7.88-7.86 (d, J=7.2 Hz, 1H), 7.57-7.52 (m, 1H), 7.30-7.26 (m, 1H), 7.18-7.14 (m, 2H), 7.10-6.97 (m, 6H), 6.76-6.70 (m, 2H), 6.51-6.47 (m, 1H), 5.21-5.20 (d, J=4.8 Hz, 1H), 2.22 (s, 3H), 2.14 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) (ppm) 165.1, 160.3, 158.1, 157.8, 155.6, 153.8, 151.4, 138.0, 137.2, 135.7, 134.6, 132.2, 132.0, 130.0, 129.9, 129.8, 126.4, 124.5, 124.2, 122.6, 120.3, 120.1, 120.0, 116.5, 116.3, 115.8, 114.8, 114.6, 81.9, 19.9, 19.5.

EXAMPLE 28

[0108] The following is an example of synthesis and characterization of a compound of the present disclosure. 3-((pyridin-3-yl)methylamino)-4-fluoro-3-(3,4-dimethylphenyl)isobenzofuran-1 (3H)-one

##STR00047##

[0109] Yield 68.8%. .sup.1H NMR (400 MHz, CDCl.sub.3) (ppm) 8.19 (br s, 1H), 7.53-7.51 (d, J=6.8 Hz, 2H), 7.45-7.38 (m, 2H), 7.22-7.20 (d, J=7.6 Hz, 1H), 7.15 (s, 1H), 7.13-7.09 (t, J=7.6 Hz, 2H), 6.94-6.91 (m, 1H), 4.64-4.60 (d, J=15.6 Hz, 1H), 4.04-4.00 (d, J=15.6 Hz, 1H), 2.24 (s, 3H), 2.19 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) (ppm) 166.6, 158.2 & 155.6 (1C,C-F), 148.2, 146.0, 137.6, 137.2, 135.3 & 135.2 (1C), 135.1, 134.2, 133.2, 131.6&131.5 (1C), 129.8, 127.3, 123.8, 123.2, 120.3 & 120.1 (1C), 119.1, 90.6, 40.6, 19.9, 19.5. ESI-MS m/z 363.1 [M+H].sup.+.

EXAMPLE 29

[0110] The following is an example of synthesis and characterization of a compound of the present disclosure. 3-(2-methoxyethylamino)-4-fluoro-3-(3,4-dimethylphenyl)isobenzofuran-1 (3H)-one

##STR00048##

[0111] Yield 61.4%. .sup.1H NMR (400 MHz, CDCl.sub.3) (ppm) 7.64-7.62 (d, J=7.2 Hz, 1H), 7.45-7.40 (m, 1H), 7.16-7.09 (m, 4H), 6.07 (br s, 1H), 4.02-3.99 (d, J=14.0 Hz, 1H), 3.67-3.62 (t d, J1=10.0 Hz, J2=1.6 Hz, 1H), 3.38 (s, 3H), 3.01-2.93 (m, 1H), 2.23 (s, 3H), 2.22 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) (ppm) 166.9, 158.1, 155.6, 137.1 & 136.9 (1C), 135.7, 134.9 & 134.8 (1C), 132.8, 131.4 & 131.3 (1C), 129.9, 127.2, 123.8, 120.2 & 120.0 (1C), 119.2 & 119.2 (1C), 89.4, 70.9, 58.9, 39.3, 19.9, 19.5. ESI-MS m/z 352.1[M+Na].sup.+.

EXAMPLE 30

[0112] The following is an example of synthesis and characterization of a compound of the present disclosure. 3-(2-methoxyphenylamino)-4-fluoro-3-(3,4-dimethylphenyl)isobenzofuran-1 (3H)-one

##STR00049##

[0113] Yield 98.8%. .sup.1H NMR (400 MHz, DMSO-d6) (ppm) 7.68-7.62 (m, 2H), 7.51 (br s, 1H), 7.43-7.39 (m, 1H), 7.27-7.23 (t, J=7.6 Hz, 1H), 7.07-7.04 (m, 2H), 6.97-6.93 (t, J=7.6 Hz, 2H), 6.90-6.86 (t, J=7.2 Hz, 1H), 3.49 (s, 3H), 2.13 (s, 3H), 2.09 (s, 3H). .sup.13C NMR (100 MHz, DMSO-d6) (ppm) 164.7, 158.1, 156.2, 155.6136.3, 136.1, 135.5, 133.9, 132.5, 130.4, 129.6, 129.0, 127.5, 124.1, 120.6, 120.4, 120.2, 119.5, 112.6, 90.9, 55.8, 19.9, 19.5. ESI-MS m/z 400.1 [M+Na].sup.+.

EXAMPLE 31

[0114] The following is an example of synthesis and characterization of a compound of the present disclosure. Methyl 2-(1,3-dihydro-1-(4-methoxyphenyl)-3-oxoisobenzofuran-1-ylamino)benzoate

##STR00050##

[0115] Yield 43.9%. .sup.1H NMR (400 MHz, CDCl.sub.3) (ppm) 9.15 (br s, 1H), 7.93-7.89 (t, J=8.4 Hz, 2H), 7.66-7.62 (t, J=7.2 Hz, 1H), 7.53-7.47 (m, 4H), 7.15-7.11 (t, J=8.0 Hz, 1H), 6.84-6.79 (m, 3H), 6.73-6.69 Hz, 1H), 3.84 (s, 3H), 3.73 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) (ppm) 169.5, 169.0, 159.9, 151.6, 146.5, 135.3, 133.9, 131.2, 130.7, 130.2, 126.9, 1326.0, 125.1, 122.1, 118.2, 117.5, 114.6, 112.9. 96.4, 55.2, 51.9. ESI-MS m/z 412.0 [M+Na].sup.+.

EXAMPLE 32

[0116] The following is an example of synthesis and characterization of a compound of the present disclosure. 3-((pyridin-2-yl)methylamino)-3-(4-methoxyphenyl)isobenzofuran-1 (3H)-one

##STR00051##

[0117] Yield 46.0%. .sup.1H NMR (400 MHz, CDCl.sub.3) (ppm) 8.44-8.42 (d, J=4.8 Hz, 1H), 7.79-7.77 (d, J=7.2 Hz, 1H), 7.74-7.71 (t, J=7.6 Hz, 1H), 7.53-7.50 (t, J=7.6 Hz, 1H), 7.45-7.42 (m, 4H), 7.35-7.33 (d, J=7.6 Hz, 1H), 7.24-7.22 (m, 1H), 6.89-6.88 (d, J=8.4 Hz, 2H), 5.06-5.02 (d, J=12.0 Hz, 1H), 4.19-4.15 (d, J=12.0 Hz, 1H), 3.80 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) 6 (ppm) 168.1, 159.6, 156.5, 147.9, 138.1, 132.9, 132.2, 129.4, 128.8, 127.8, 123.3, 122.9, 122.8, 122.6, 113.9, 90.4, 55.3, 44.6. ESI-MS m/z 369.0 [M+Na].sup.+.

EXAMPLE 33

[0118] The following is an example of synthesis and characterization of a compound of the present disclosure. 3-(5-methyl-4-phenylthiazol-2-ylamino)-4-fluoro-3-p-tolylisobenzofuran-1 (3H)-one

##STR00052##

[0119] Yield 38.3%. .sup.1H NMR (400 MHz, CDCl.sub.3) (ppm) 7.80-7.78 (d, J=7.2 Hz, 1H), 7.56-7.51 (m, 1H), 7.43-7.41 (d, J=8.0 Hz, 2H), 7.40-7.35 (m, 4H), 7.32-7.30 (m, 1H), 7.26-7.21 (t, J=8.8 Hz, 1H), 7.16-7.14 (d, J=8.0 Hz, 2H), 2.51 (s, 3H), 2.31 (s, 3H). .sup.13C NMR (100 MHz, CDCl.sub.3) (ppm) 164.1, 158.4, 155.9, 152.0, 144.9, 138.5, 136.3, 134.4, 132.2 & 132.2 (1C), 131.3, 129.3, 128.3, 128.1, 127.5, 125.4, 122.3, 121.7, 121.5, 120.3, 92.9, 21.1, 12.3. ESI-MS m/z 431.0 [M+H].sup.+.

EXAMPLE 34

[0120] The following is an example of synthesis and characterization of a compound of the present disclosure.

##STR00053##

[0121] To a solution of compound 3 in DMF was added 2-fluoro-6-(phenylamino)benzoic acid, followed by EDC and HOBt. The reaction mixture was allowed to sit at 40 C. for 24 h, and then concentrated. After removing the solvent, the crude material was purified by flash column chromatograph to get the final compound.

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