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PRODUCTION OF STEVIOL GLYCOSIDES IN RECOMBINANT HOSTS

20190144907 ยท 2019-05-16

    Inventors

    Cpc classification

    International classification

    Abstract

    The invention relates to recombinant microorganisms and methods for producing steviol glycosides, glycosides of steviol precursors, and steviol glycoside precursors.

    Claims

    1. A recombinant host cell capable of producing one or more steviol glycosides and/or glycosylated steviol precursors, or a composition thereof, comprising: (a) a gene encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-19 carboxyl position; (b) a gene encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-13 hydroxyl position; (c) a gene encoding a polypeptide capable of beta-1,2-glycosylation of the C2 and/or beta-1,3-glycosylation of the C3 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside; and/or (d) a gene encoding a polypeptide capable of glycosylating a steviol precursor at its C-19 carboxyl or C-19 hydroxyl position; wherein at least one of the genes is a recombinant gene.

    2. The recombinant host cell of claim 1, wherein: (a) the polypeptide capable of glycosylating steviol or a steviol glycoside at its C-19 carboxyl position is a UGT73C1 polypeptide, a UGT73C3 polypeptide, a UGT73C5 polypeptide, a UGT73C6 polypeptide, a UGT73E1 polypeptide, a UGT75B1 polypeptide, a UGT75L6 polypeptide, a Olel polypeptide, a UGT5 polypeptide, a SA Gtase polypeptide, a UDPG1 polypeptide, a UN1671 polypeptide, a UGT74F1 polypeptide, a UGT84B2 polypeptide, and/or a UGT74F2-like UGT polypeptide; (b) the polypeptide capable of glycosylating steviol or a steviol glycoside at its C-13 hydroxyl position is a UGT73C1 polypeptide, a UGT73C3 polypeptide, a UGT73C5 polypeptide, a UGT73C6 polypeptide, a UGT73C7 polypeptide, a UGT73E1 polypeptide, and/or a UGT76E12 polypeptide; (c) the polypeptide capable of beta-1,2-glycosylation of the C2 and/or beta-1,3-glycosylation of the C3 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside is a UGT73C6 polypeptide, a CaUGT3 polypeptide, a UN32491 polypeptide, and/or a UN1671 polypeptide; and/or (d) the polypeptide capable of glycosylating a steviol precursor at its C-19 carboxyl or C-19 hydroxyl position is a UGT73C1 polypeptide, a UGT73C3 polypeptide, a UGT73C5 polypeptide, a UGT73C6 polypeptide, a UGT73E1 polypeptide, a UGT74D1 polypeptide, a UGT75B1 polypeptide, a UGT75L6 polypeptide, a UGT76E12 polypeptide, a Olel polypeptide, a UGT5 polypeptide, a SA Gtase, a UDPG1 polypeptide, a UGT74F1 polypeptide, a UGT75D1 polypeptide, a UGT84B2 polypeptide, a CaUGT2 polypeptide, and/or a UGT74F2-like UGT polypeptide.

    3. The recombinant host cell of claim 2, wherein: the UGT73C1 polypeptide comprises a polypeptide having at least 60% identity to an amino acid sequence set forth in SEQ ID NO:127, the UGT73C3 polypeptide comprises a polypeptide having at least 60% identity to an amino acid sequence set forth in SEQ ID NO:133, the UGT73C5 polypeptide comprises a polypeptide having at least 60% identity to an amino acid sequence set forth in SEQ ID NO:135, the UGT73C6 polypeptide comprises a polypeptide having at least 60% identity to an amino acid sequence set forth in SEQ ID NO:137, the UGT73E1 polypeptide comprises a polypeptide having at least 50% identity to an amino acid sequence set forth in SEQ ID NO:141, the UGT74D1 polypeptide comprises a polypeptide having at least 50% identity to an amino acid sequence set forth in SEQ ID NO:143, the UGT75B1 polypeptide comprises a polypeptide having at least 50% sequence identity to an amino acid sequence set forth in SEQ ID NO:145, the UGT75L6 polypeptide comprises a polypeptide having at least 60% sequence identity to an amino acid sequence set forth in SEQ ID NO:147, the UGT76E12 polypeptide comprises a polypeptide having at least 60% sequence identity to an amino acid sequence set forth in SEQ ID NO:153, the Olel polypeptide comprises a polypeptide having at least 55% identity to an amino acid sequence set forth in SEQ ID NO:177, the UGT5 polypeptide comprises a polypeptide having at least 65% identity to an amino acid sequence set forth in SEQ ID NO:181, the SA Gtase polypeptide comprises a polypeptide having at least 55% identity to an amino acid sequence set forth in SEQ ID NO:183, the UDPG1 polypeptide comprises a polypeptide having at least 50% sequence identity to an amino acid sequence set forth in SEQ ID NO:185, the UN1671 polypeptide comprises a polypeptide having at least 45% identity to an amino acid sequence set forth in SEQ ID NO:201, the UGT74F1 polypeptide comprises a polypeptide having at least 50% sequence identity to an amino acid sequence set forth in SEQ ID NO:203, the UGT75D1 polypeptide comprises a polypeptide having at least 50% sequence identity to an amino acid sequence set forth in SEQ ID NO:205, the UGT84B2 polypeptide comprises a polypeptide having at least 40% sequence identity to an amino acid sequence set forth in SEQ ID NO:207, the UGT74F2-like UGT polypeptide comprises a polypeptide having at least 55% identity to an amino acid sequence set forth in SEQ ID NO:211, the UGT73C7 polypeptide comprises a polypeptide having at least 60% identity to an amino acid sequence set forth in SEQ ID NO:139, the CaUGT3 polypeptide comprises a polypeptide having at least 50% identity to an amino acid sequence set forth in SEQ ID NO:169, the UN32491 polypeptide comprises a polypeptide having at least 50% identity to an amino acid sequence set forth in SEQ ID NO:199, and/or the CaUGT2 polypeptide comprises a polypeptide having at least 55% identity to an amino acid sequence set forth in SEQ ID NO:209.

    4. The recombinant host cell of any one of claims 1-3, wherein the recombinant host cell further comprises: (a) a gene encoding a polypeptide capable of synthesizing geranylgeranyl pyrophosphate (GGPP) from farnesyl diphosphate (FPP) and isopentenyl diphosphate (IPP); (b) a gene encoding a polypeptide capable of synthesizing ent-copalyl diphosphate from GGPP; (c) a gene encoding an a polypeptide capable of synthesizing ent-kaurene from ent-copalyl diphosphate; (d) a gene encoding a polypeptide capable of synthesizing ent-kaurenoic acid from ent-kaurene; (e) a gene encoding a polypeptide capable of reducing cytochrome P450 complex; (f) a gene encoding a polypeptide capable of synthesizing steviol from ent-kaurenoic acid; (g) a gene encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-13 hydroxyl position thereof; (h) a gene encoding a polypeptide capable of beta 1,3 glycosylation of the C3 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside; (i) a gene encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-19 carboxyl position; and/or (k) a gene encoding a polypeptide capable of beta 1,2 glycosylation of the C2 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside; wherein at least one of the genes is a recombinant gene.

    5. The recombinant host cell of claim 4, wherein: (a) the polypeptide capable of synthesizing GGPP comprises a polypeptide having at least 70% sequence identity to the amino acid sequence set forth in SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:24, SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:30, SEQ ID NO:32, or SEQ ID NO:116; (b) the polypeptide capable of synthesizing ent-copalyl diphosphate comprises a polypeptide having at least 70% sequence identity to the amino acid sequence set forth in SEQ ID NO:34, SEQ ID NO:36, SEQ ID NO:38, SEQ ID NO:40, SEQ ID NO:42, or SEQ ID NO:120; (c) the polypeptide capable of synthesizing ent-kaurene comprises a polypeptide having at least 70% sequence identity to the amino acid sequence set forth in SEQ ID NO:44, SEQ ID NO:46, SEQ ID NO:48, SEQ ID NO:50, or SEQ ID NO:52; (d) the polypeptide capable of synthesizing ent-kaurenoic acid comprises a polypeptide having at least 70% sequence identity to the amino acid sequence set forth in SEQ ID NO:60, SEQ ID NO:62, SEQ ID NO:117, SEQ ID NO:66, SEQ ID NO:68, SEQ ID NO:70, SEQ ID NO:72, SEQ ID NO:74, or SEQ ID NO:76; (e) the polypeptide capable of reducing cytochrome P450 complex comprises a polypeptide having at least 70% sequence identity to the amino acid sequence set forth in SEQ ID NO:78, SEQ ID NO:80, SEQ ID NO:82, SEQ ID NO:84, SEQ ID NO:86, SEQ ID NO:88, SEQ ID NO:90, SEQ ID NO:92; (f) the polypeptide capable of synthesizing steviol comprises a polypeptide having at least 70% sequence identity to the amino acid sequence set forth in SEQ ID NO:94, SEQ ID NO:97, SEQ ID NO:100, SEQ ID NO:101, SEQ ID NO:102, SEQ ID NO:103, SEQ ID NO:104, SEQ ID NO:106, SEQ ID NO:108, SEQ ID NO:110, SEQ ID NO:112, or SEQ ID NO:114; (g) the polypeptide capable of glycosylating steviol or a steviol glycoside at its C-13 hydroxyl position thereof comprises a polypeptide having at least 55% sequence identity to the amino acid sequence set forth in SEQ ID NO:7; (h) the polypeptide capable of beta 1,3 glycosylation of the C3 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside comprises a polypeptide having at least 50% sequence identity to the amino acid sequence set forth in SEQ ID NO:9; (i) the polypeptide capable of glycosylating steviol or a steviol glycoside at its C-19 carboxyl position comprises a polypeptide having at least 55% sequence identity to the amino acid sequence set forth in SEQ ID NO:4; and/or (k) the polypeptide capable of beta 1,2 glycosylation of the C2 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside comprises a polypeptide having 80% or greater identity to the amino acid sequence set forth in SEQ ID NO:11; a polypeptide having 80% or greater identity to the amino acid sequence set forth in SEQ ID NO:13; or a polypeptide having at least 65% sequence identity to the amino acid sequence set forth in SEQ ID NO:16.

    6. The recombinant host cell of any of claims 1-5, wherein expression of the one or more recombinant genes increases an amount of the one or more steviol glycosides and/or glycosylated steviol precursors, or a composition thereof accumulated by the cell relative to a corresponding host lacking the one or more recombinant genes.

    7. The recombinant host cell of claim 6, wherein expression of the one or more recombinant genes increases the amount of the one or more steviol glycosides and/or glycosylated steviol precursors, or the composition thereof, accumulated by the cell by at least about 5%, at least about 10%, at least about 25%, at least about 50%, at least about 75%, or at least about 100% relative to a corresponding host lacking the one or more recombinant genes.

    8. The recombinant host cell of claim 6 or 7, wherein expression of the one or more recombinant genes increases the amount of ent-kaurenoic acid+2Glc (#7), ent-kaurenoic acid+3Glc (isomer 1), ent-kaurenoic acid+3Glc (isomer 2), steviol-13-O-glucoside (13-SMG), Rebaudioside A (RebA), Rebaudioside B (RebB), Steviol+4Glc (#36), Steviol+6Glc (isomer 1), Steviol+7Glc (isomer 2), and/or ent-Kaurenol+3Glc (isomer 1 and/or isomer 2) accumulated by the cell relative to a corresponding host lacking the one or more recombinant genes.

    9. The recombinant host cell of any one of claims 1-8, wherein the one or more steviol glycosides and/or glycosylated steviol precursors are, or the composition thereof comprises, steviol-13-O-glucoside (13-SMG), steviol-19-O-glucoside (19-SMG), steviol-1,2-bioside, steviol-1,3-bioside, 1,2-stevioside, 1,3-stevioside, rubusoside, Rebaudioside A (RebA), Rebaudioside B (RebB), Rebaudioside C (RebC), Rebaudioside D (RebD), Rebaudioside E (RebE), Rebaudioside F (RebF), Rebaudioside M (RebM), Rebaudioside Q (RebQ), Rebaudioside I (RebI), dulcoside A, a mono-glycosylated ent-kaurenoic acid, a di-glycosylated ent-kaurenoic acid, a tri-glycosylated ent-kaurenoic acid, a mono-glycosylated ent-kaurenols, a di-glycosylated ent-kaurenol, a tri-glycosylated ent-kaurenol, a tri-glycosylated steviol glycoside, a tetra-glycosylated steviol glycoside, a penta-glycosylated steviol glycoside, a hexa-glycosylated steviol glycoside, a hepta-glycosylated steviol glycoside, or an isomer thereof.

    10. The recombinant host cell of claim 9, wherein the mono-glycosylated ent-kaurenoic acid comprises KA1.58 of Table 1 and/or the penta-glycosylated steviol comprises Compound 5.24 of Table 1.

    11. The recombinant host cell of claim 1-10, wherein the recombinant host cell comprises a plant cell, a mammalian cell, an insect cell, a fungal cell, an algal cell, or a bacterial cell.

    12. A method of producing in a cell culture one or more steviol glycosides and/or glycosylated steviol precursors, or a composition thereof, comprising growing the recombinant host cell of any one of claims 1-11 in the cell culture, under conditions in which the genes are expressed, and wherein the one or more steviol glycosides and/or glycosylated steviol precursors, or the composition thereof is produced by the recombinant host cell.

    13. The method of claim 12, wherein the genes are constitutively expressed and/or expression of the genes is induced.

    14. The method of claim 12 or 13, wherein an amount of ent-kaurenoic acid+2Glc (#7), ent-kaurenoic acid+3Glc (isomer 1), ent-kaurenoic acid+3Glc (isomer 2), 13-SMG, RebA, RebB, Steviol+4Glc (#36), Steviol+6Glc (isomer 1), Steviol+7Glc (isomer 2), and/or ent-Kaurenol+3Glc (isomer 1 and/or isomer 2) accumulated by the recombinant host cell is increased by at least about 5% relative to a corresponding host lacking the one or more recombinant genes.

    15. The method of any one of claims 12-14, further comprising isolating from the cell cultures the one or more steviol glycosides and/or glycosylated steviol precursors or the composition thereof produced thereby.

    16. The method of claim 15, wherein the isolating step comprises: (a) providing the cell culture comprising the one or more steviol glycosides and/or glycosylated steviol precursors, or the composition thereof; (b) separating a liquid phase of the cell culture from a solid phase of the cell culture to obtain a supernatant comprising the produced one or more steviol glycosides and/or glycosylated steviol precursors, or the composition thereof; (c) providing one or more adsorbent resins, comprising providing the adsorbent resins in a packed column; and (d) contacting the supernatant of step (b) with the one or more adsorbent resins in order to obtain at least a portion of the produced one or more steviol glycosides and/or glycosylated steviol precursors, or the composition thereof, thereby isolating the produced one or more steviol glycosides or the steviol glycoside composition; or (a) providing the cell culture comprising the one or more steviol glycosides and/or glycosylated steviol precursors, or the composition thereof; (b) separating a liquid phase of the cell culture from a solid phase of the cell culture to obtain a supernatant comprising the produced one or more steviol glycosides and/or glycosylated steviol precursors, or the composition thereof; (c) providing one or more ion exchange or ion exchange or reversed-phase chromatography columns; and (d) contacting the supernatant of step (b) with the one or more ion exchange or ion exchange or reversed-phase chromatography columns in order to obtain at least a portion of the produced one or more steviol glycosides and/or glycosylated steviol precursors, or the composition thereof, thereby isolating the produced one or more steviol glycosides and/or glycosylated steviol precursors, or the composition thereof; or (a) providing the cell culture comprising the one or more steviol glycosides and/or glycosylated steviol precursors, or the composition thereof; (b) separating a liquid phase of the cell culture from a solid phase of the cell culture to obtain a supernatant comprising the produced one or more steviol glycosides and/or glycosylated steviol precursors, or the composition thereof; (c) crystallizing or extracting the produced one or more steviol glycosides and/or glycosylated steviol precursors, or the composition thereof, thereby isolating the produced one or more steviol glycosides and/or glycosylated steviol precursors, or the composition thereof.

    17. The method of any one of claims 12-14, further comprising recovering from the cell culture the one or more steviol glycosides and/or glycosylated steviol precursors or the composition thereof from the cell culture, wherein the cell culture is enriched for the one or more steviol glycosides and/or glycosides of a steviol precursor, or the composition thereof relative to a steviol glycoside composition from a Stevia plant and has a reduced level of Stevia plant-derived components relative to a plant-derived Stevia extract.

    18. The method of claim 17, wherein the recovered one or more steviol glycosides and/or glycosylated steviol precursors, or the composition thereof are present in relative amounts that are different from a steviol glycoside composition recovered from a Stevia plant and have a reduced level of Stevia plant-derived components relative to a plant-derived Stevia extract.

    19. A method for producing one or more steviol glycosides and/or glycosylated steviol precursors, or the composition thereof, comprising whole cell bioconversion of plant-derived or synthetic steviol, steviol precursors and/or steviol glycosides in a cell culture medium of a recombinant host cell using: (a) a gene encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-19 carboxyl position; (b) a gene encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-13 hydroxyl position; (c) a gene encoding a polypeptide capable of beta-1,2-glycosylation of the C2 and/or beta-1,3-glycosylation of the C3 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside; and/or (d) a gene encoding a polypeptide capable of glycosylating a steviol precursor at its C-19 carboxyl or C-19 hydroxyl position; wherein at least one of the polypeptides is a recombinant polypeptide expressed in the recombinant host cell; and producing the one or more steviol glycosides and/or glycosylated steviol precursors, or the composition thereof, thereby.

    20. The method of claim 19, wherein: (a) the polypeptide capable of glycosylating steviol or a steviol glycoside at its C-19 carboxyl position is a UGT73C1 polypeptide, a UGT73C3 polypeptide, a UGT73C5 polypeptide, a UGT73C6 polypeptide, a UGT73E1 polypeptide, a UGT75B1 polypeptide, a UGT75L6 polypeptide, a Olel polypeptide, a UGT5 polypeptide, a SA Gtase polypeptide, a UDPG1 polypeptide, a UN1671 polypeptide, a UGT74F1 polypeptide, a UGT84B2 polypeptide, and/or a UGT74F2-like UGT polypeptide; (b) the polypeptide capable of glycosylating steviol or a steviol glycoside at its C-13 hydroxyl position is a UGT73C1 polypeptide, a UGT73C3 polypeptide, a UGT73C5 polypeptide, a UGT73C6 polypeptide, a UGT73C7 polypeptide, a UGT73E1 polypeptide, and/or a UGT76E12 polypeptide; (c) the polypeptide capable of beta-1,2-glycosylation of the C2 and/or beta-1,3-glycosylation of the C3 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside is a UGT73C6 polypeptide, a CaUGT3 polypeptide, a UN32491 polypeptide, and/or a UN1671 polypeptide; and/or (d) the polypeptide capable of glycosylating a steviol precursor at its C-19 carboxyl or C-19 hydroxyl position is a UGT73C1 polypeptide, a UGT73C3 polypeptide, a UGT73C5 polypeptide, a UGT73C6 polypeptide, a UGT73E1 polypeptide, a UGT75B1 polypeptide, a UGT75L6 polypeptide, a UGT76E12 polypeptide, a Olel polypeptide, a UGT5 polypeptide, a SA Gtase, a UDPG1 polypeptide, a UGT74F1 polypeptide, a UGT75D1 polypeptide, a UGT84B2 polypeptide, and/or a UGT74F2-like UGT polypeptide.

    21. The method of claim 20, wherein: the UGT73C1 polypeptide comprises a polypeptide having at least 60% identity to an amino acid sequence set forth in SEQ ID NO:127, the UGT73C3 polypeptide comprises a polypeptide having at least 60% identity to an amino acid sequence set forth in SEQ ID NO:133, the UGT73C5 polypeptide comprises a polypeptide having at least 60% identity to an amino acid sequence set forth in SEQ ID NO:135, the UGT73C6 polypeptide comprises a polypeptide having at least 60% identity to an amino acid sequence set forth in SEQ ID NO:137, the UGT73E1 polypeptide comprises a polypeptide having at least 50% identity to an amino acid sequence set forth in SEQ ID NO:141, a UGT74D1 polypeptide comprises a polypeptide having at least 50% identity to an amino acid sequence set forth in SEQ ID NO:143, the UGT75B1 polypeptide comprises a polypeptide having at least 50% sequence identity to an amino acid sequence set forth in SEQ ID NO:145, the UGT75L6 polypeptide comprises a polypeptide having at least 60% sequence identity to an amino acid sequence set forth in SEQ ID NO:147, the UGT76E12 polypeptide comprises a polypeptide having at least 60% sequence identity to an amino acid sequence set forth in SEQ ID NO:153, the Olel polypeptide comprises a polypeptide having at least 55% identity to an amino acid sequence set forth in SEQ ID NO:177, the UGT5 polypeptide comprises a polypeptide having at least 65% identity to an amino acid sequence set forth in SEQ ID NO:181, the SA Gtase polypeptide comprises a polypeptide having at least 55% identity to an amino acid sequence set forth in SEQ ID NO:183, the UDPG1 polypeptide comprises a polypeptide having at least 50% sequence identity to an amino acid sequence set forth in SEQ ID NO:185, the UN1671 polypeptide comprises a polypeptide having at least 45% identity to an amino acid sequence set forth in SEQ ID NO:201, the UGT74F1 polypeptide comprises a polypeptide having at least 50% sequence identity to an amino acid sequence set forth in SEQ ID NO:203, the UGT75D1 polypeptide comprises a polypeptide having at least 50% sequence identity to an amino acid sequence set forth in SEQ ID NO:205, the UGT84B2 polypeptide comprises a polypeptide having at least 40% sequence identity to an amino acid sequence set forth in SEQ ID NO:207, the UGT74F2-like UGT polypeptide comprises a polypeptide having at least 55% identity to an amino acid sequence set forth in SEQ ID NO:211, the UGT73C7 polypeptide comprises a polypeptide having at least 60% identity to an amino acid sequence set forth in SEQ ID NO:139, the CaUGT3 polypeptide comprises a polypeptide having at least 50% identity to an amino acid sequence set forth in SEQ ID NO:169, the UN32491 polypeptide comprises a polypeptide having at least 50% identity to an amino acid sequence set forth in SEQ ID NO:199, or the CaUGT2 polypeptide comprises a polypeptide having at least 55% identity to an amino acid sequence set forth in SEQ ID NO:209.

    22. The method of any one of claims 12-21, wherein the recombinant host cell is a plant cell, a mammalian cell, an insect cell, a fungal cell, an algal cell or a bacterial cell.

    23. An in vitro method for producing one or more steviol glycosides and/or glycosylated steviol precursors, or a composition thereof comprising adding: (a) a UGT85C2 polypeptide having at least 55% identity to an amino acid sequence set forth in SEQ ID NO:7; (b) a UGT76G1 polypeptide having at least 50% identity to an amino acid sequence set forth in SEQ ID NO:9; (c) a UGT74G1 polypeptide having at least 55% identity to an amino acid sequence set forth in SEQ ID NO:4; (d) a UGT91D2 functional homolog polypeptide comprising a UGT91D2e polypeptide having 90% or greater identity to an amino acid sequence set forth in SEQ ID NO:11 or a UGT91D2e-b polypeptide having 90% or greater identity to an amino acid sequence set forth in SEQ ID NO:13; (e) a EUGT11 polypeptide having at least 65% identity to an amino acid sequence set forth in SEQ ID NO:16; and/or (f) a UGT73C1 polypeptide comprises a polypeptide having at least 60% identity to an amino acid sequence set forth in SEQ ID NO:127, a UGT73C3 polypeptide comprises a polypeptide having at least 60% identity to an amino acid sequence set forth in SEQ ID NO:133, a UGT73C5 polypeptide comprises a polypeptide having at least 60% identity to an amino acid sequence set forth in SEQ ID NO:135, a UGT73C6 polypeptide comprises a polypeptide having at least 60% identity to an amino acid sequence set forth in SEQ ID NO:137, a UGT73E1 polypeptide comprises a polypeptide having at least 50% identity to an amino acid sequence set forth in SEQ ID NO:141, a UGT74D1 polypeptide comprises a polypeptide having at least 50% identity to an amino acid sequence set forth in SEQ ID NO:143, a UGT75B1 polypeptide comprises a polypeptide having at least 50% sequence identity to an amino acid sequence set forth in SEQ ID NO:145, a UGT75L6 polypeptide comprises a polypeptide having at least 60% sequence identity to an amino acid sequence set forth in SEQ ID NO:147, a UGT76E12 polypeptide comprises a polypeptide having at least 60% sequence identity to an amino acid sequence set forth in SEQ ID NO:153, a Olel polypeptide comprises a polypeptide having at least 55% identity to an amino acid sequence set forth in SEQ ID NO:177, a UGTS polypeptide comprises a polypeptide having at least 65% identity to an amino acid sequence set forth in SEQ ID NO:181, a SA Gtase polypeptide comprises a polypeptide having at least 55% identity to an amino acid sequence set forth in SEQ ID NO:183, a UDPG1 polypeptide comprises a polypeptide having at least 50% sequence identity to an amino acid sequence set forth in SEQ ID NO:185, a UN1671 polypeptide comprises a polypeptide having at least 45% identity to an amino acid sequence set forth in SEQ ID NO:201, a UGT74F1 polypeptide comprises a polypeptide having at least 50% sequence identity to an amino acid sequence set forth in SEQ ID NO:203, a UGT75D1 polypeptide comprises a polypeptide having at least 50% sequence identity to an amino acid sequence set forth in SEQ ID NO:205, a UGT84B2 polypeptide comprises a polypeptide having at least 40% sequence identity to an amino acid sequence set forth in SEQ ID NO:207, a UGT74F2-like UGT polypeptide comprises a polypeptide having at least 55% identity to an amino acid sequence set forth in SEQ ID NO:211, a UGT73C7 polypeptide comprises a polypeptide having at least 60% identity to an amino acid sequence set forth in SEQ ID NO:139, a CaUGT3 polypeptide comprises a polypeptide having at least 50% identity to an amino acid sequence set forth in SEQ ID NO:169, a UN32491 polypeptide comprises a polypeptide having at least 50% identity to an amino acid sequence set forth in SEQ ID NO:199, or a CaUGT2 polypeptide comprises a polypeptide having at least 55% identity to an amino acid sequence set forth in SEQ ID NO:209; and a plant-derived or synthetic steviol glycoside precursor or a plant-derived or synthetic steviol precursor to a reaction mixture; wherein at least one of the polypeptides is a recombinant polypeptide; and producing the one or more steviol glycosides and/or glycosylated steviol precursors, or the composition thereof, thereby.

    24. The method of claim 23, wherein the reaction mixture comprises: (a) glucose, fructose, sucrose, xylose, rhamnose, uridine diphosphate (UDP)-glucose, UDP-rhamnose, UDP-xylose, and/or N-acetyl-glucosamine; and/or (b) reaction buffer and/or salts.

    25. The method of any one of claims 12-24, wherein the one or more steviol glycosides and/or glycosylated steviol precursors are, or the composition thereof comprises, 13-SMG, 19-SMG, steviol-1,2-bioside, steviol-1,3-bioside, 1,2-stevioside, 1,3-stevioside, rubusoside, RebA, RebB, RebC, RebD, RebE, RebF, RebM, RebQ, RebI, dulcoside A, a mono-glycosylated ent-kaurenoic acid, a di-glycosylated ent-kaurenoic acid, a tri-glycosylated ent-kaurenoic acid, a mono-glycosylated ent-kaurenols, a di-glycosylated ent-kaurenol, a tri-glycosylated ent-kaurenol, a tri-glycosylated steviol glycoside, a tetra-glycosylated steviol glycoside, a penta-glycosylated steviol glycoside, a hexa-glycosylated steviol glycoside, a hepta-glycosylated steviol glycoside, or an isomer thereof.

    26. The method of claim 25, wherein the mono-glycosylated ent-kaurenoic acid comprises KA1.58 of Table 1 and/or the penta-glycosylated steviol comprises Compound 5.24 of Table 1.

    27. A cell culture, comprising the recombinant host cell of any one of claims 1-11, the cell culture further comprising: (a) one or more steviol glycosides and/or glycosylated steviol precursors, or the composition thereof produced by the recombinant host cell, (b) glucose, fructose, sucrose, xylose, rhamnose, UDP-glucose, UDP-rhamnose, UDP-xylose, and/or N-acetyl-glucosamine; and (c) supplemental nutrients comprising trace metals, vitamins, salts, yeast nitrogen base (YNB), and/or amino acids; wherein the one or more steviol glycosides and/or glycosylated steviol precursors, or the composition thereof is present at a concentration of at least 1 mg/liter of the cell culture; wherein the cell culture is enriched for the one or more steviol glycosides and/or glycosides of a steviol precursor, or the composition thereof relative to a steviol glycoside composition from a Stevia plant and has a reduced level of Stevia plant-derived components relative to a plant-derived Stevia extract.

    28. A cell lysate from the recombinant host cell of any one of claims 1-11 grown in the cell culture, comprising: (a) one or more steviol glycosides and/or glycosylated steviol precursors, or the composition thereof produced by the recombinant host cell; (b) glucose, fructose, sucrose, xylose, rhamnose, UDP-glucose, UDP-rhamnose, UDP-xylose, and/or N-acetyl-glucosamine; and/or (c) supplemental nutrients comprising trace metals, vitamins, salts, yeast nitrogen base, YNB, and/or amino acids; wherein the one or more steviol glycosides and/or glycosylated steviol precursors, or the composition thereof produced by the recombinant host cell is present at a concentration of at least 1 mg/liter of the cell culture.

    29. A reaction mixture, comprising: (a) a UGT85C2 polypeptide having at least 55% identity to an amino acid sequence set forth in SEQ ID NO:7; (b) a UGT76G1 polypeptide having at least 50% identity to an amino acid sequence set forth in SEQ ID NO:9; (c) a UGT74G1 polypeptide having at least 55% identity to an amino acid sequence set forth in SEQ ID NO:4; (d) a UGT91D2 functional homolog polypeptide comprising a UGT91D2e polypeptide having 90% or greater identity to an amino acid sequence set forth in SEQ ID NO:11 or a UGT91D2e-b polypeptide having 90% or greater identity to an amino acid sequence set forth in SEQ ID NO:13; (e) a EUGT11 polypeptide having at least 65% identity to an amino acid sequence set forth in SEQ ID NO:16; and/or (f) a UGT73C1 polypeptide comprises a polypeptide having at least 60% identity to an amino acid sequence set forth in SEQ ID NO:127, a UGT73C3 polypeptide comprises a polypeptide having at least 60% identity to an amino acid sequence set forth in SEQ ID NO:133, a UGT73C5 polypeptide comprises a polypeptide having at least 60% identity to an amino acid sequence set forth in SEQ ID NO:135, a UGT73C6 polypeptide comprises a polypeptide having at least 60% identity to an amino acid sequence set forth in SEQ ID NO:137, a UGT73E1 polypeptide comprises a polypeptide having at least 50% identity to an amino acid sequence set forth in SEQ ID NO:141, a UGT75B1 polypeptide comprises a polypeptide having at least 50% sequence identity to an amino acid sequence set forth in SEQ ID NO:145, a UGT75L6 polypeptide comprises a polypeptide having at least 60% sequence identity to an amino acid sequence set forth in SEQ ID NO:147, a UGT76E12 polypeptide comprises a polypeptide having at least 60% sequence identity to an amino acid sequence set forth in SEQ ID NO:153, a Olel polypeptide comprises a polypeptide having at least 55% identity to an amino acid sequence set forth in SEQ ID NO:177, a UGT5 polypeptide comprises a polypeptide having at least 65% identity to an amino acid sequence set forth in SEQ ID NO:181, a SA Gtase polypeptide comprises a polypeptide having at least 55% identity to an amino acid sequence set forth in SEQ ID NO:183, a UDPG1 polypeptide comprises a polypeptide having at least 50% sequence identity to an amino acid sequence set forth in SEQ ID NO:185, a UN1671 polypeptide comprises a polypeptide having at least 45% identity to an amino acid sequence set forth in SEQ ID NO:201, a UGT74F1 polypeptide comprises a polypeptide having at least 50% sequence identity to an amino acid sequence set forth in SEQ ID NO:203, a UGT75D1 polypeptide comprises a polypeptide having at least 50% sequence identity to an amino acid sequence set forth in SEQ ID NO:205, a UGT84B2 polypeptide comprises a polypeptide having at least 40% sequence identity to an amino acid sequence set forth in SEQ ID NO:207, a UGT74F2-like UGT polypeptide comprises a polypeptide having at least 55% identity to an amino acid sequence set forth in SEQ ID NO:211, a UGT73C7 polypeptide comprises a polypeptide having at least 60% identity to an amino acid sequence set forth in SEQ ID NO:139, a CaUGT3 polypeptide comprises a polypeptide having at least 50% identity to an amino acid sequence set forth in SEQ ID NO:169, or a UN32491 polypeptide comprises a polypeptide having at least 50% identity to an amino acid sequence set forth in SEQ ID NO:199; and further comprising: (g) one or more steviol glycosides and/or glycosylated steviol precursors, or a composition thereof; (h) glucose, fructose, sucrose, xylose, rhamnose, uridine diphosphate (UDP)-glucose, UDP-rhamnose, UDP-xylose, and/or N-acetyl-glucosamine; and/or (i) reaction buffer and/or salts.

    30. A composition of one or more steviol glycosides and/or glycosylated steviol precursors produced by the recombinant host cell of any one of claims 1-11; wherein the one or more steviol glycosides and/or glycosylated steviol precursors produced by the recombinant host cell are present in relative amounts that are different from a steviol glycoside composition from a Stevia plant and have a reduced level of Stevia plant-derived components relative to a plant-derived Stevia extract.

    31. A composition of one or more steviol glycosides and/or glycosylated steviol precursors produced by the method of any one of claims 12-26; wherein the one or more steviol glycosides and/or glycosylated steviol precursors produced by the recombinant host cell are present in relative amounts that are different from a steviol glycoside composition from a Stevia plant and have a reduced level of Stevia plant-derived components relative to a plant-derived Stevia extract.

    32. A sweetener composition, comprising one or more steviol glycosides and/or glycosylated steviol precursors of claim 30 or 31.

    33. A food product, comprising the sweetener composition of claim 32.

    34. A beverage or a beverage concentrate, comprising the sweetener composition of claim 32.

    35. An isolated nucleic acid molecule encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-19 carboxyl position or a catalytically active portion thereof, wherein the encoded polypeptide capable of glycosylating steviol or a steviol glycoside at its C-19 carboxyl position or the catalytically active portion thereof has at least 60% sequence identity to the amino acid sequence set forth in SEQ ID NO:127, at least 60% sequence identity to the amino acid sequence set forth in SEQ ID NO:133, at least 60% sequence identity to the amino acid sequence set forth in SEQ ID NO:135, at least 60% sequence identity to the amino acid sequence set forth in SEQ ID NO:137, at least 50% sequence identity to the amino acid sequence set forth in SEQ ID NO:141, at least 50% sequence identity to the amino acid sequence set forth in SEQ ID NO:145, at least 60% sequence identity to the amino acid sequence set forth in SEQ ID NO:147, at least 55% sequence identity to the amino acid sequence set forth in SEQ ID NO:177, at least 65% sequence identity to the amino acid sequence set forth in SEQ ID NO:181, at least 55% sequence identity to the amino acid sequence set forth in SEQ ID NO:183, at least 50% sequence identity to the amino acid sequence set forth in SEQ ID NO:185, at least 45% sequence identity to the amino acid sequence set forth in SEQ ID NO:201, at least 50% sequence identity to the amino acid sequence set forth in SEQ ID NO:203, at least 40% sequence identity to the amino acid sequence set forth in SEQ ID NO:207, or at least 55% sequence identity to the amino acid sequence set forth in SEQ ID NO:211.

    36. An isolated nucleic acid molecule encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-13 hydroxyl position or a catalytically active portion thereof, wherein the encoded polypeptide capable of glycosylating steviol or a steviol glycoside at its C-13 hydroxyl position or the catalytically active portion thereof has at least 60% sequence identity to the amino acid sequence set forth in SEQ ID NO:127, at least 60% sequence identity to the amino acid sequence set forth in SEQ ID NO:133, at least 60% sequence identity to the amino acid sequence set forth in SEQ ID NO:135, at least 60% sequence identity to the amino acid sequence set forth in SEQ ID NO:137, at least 60% sequence identity to the amino acid sequence set forth in SEQ ID NO:139, at least 50% sequence identity to the amino acid sequence set forth in SEQ ID NO:141, or at least 60% sequence identity to the amino acid sequence set forth in SEQ ID NO:153.

    37. An isolated nucleic acid molecule encoding a polypeptide capable of beta-1,2-glycosylation of the C2 and/or beta-1,3-glycosylation of the C3 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside or a catalytically active portion thereof, wherein the encoded polypeptide capable of beta-1,2-glycosylation of the C2 and/or beta-1,3-glycosylation of the C3 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside or the catalytically active portion thereof has at least 60% sequence identity to the amino acid sequence set forth in SEQ ID NO:137, at least 50% sequence identity to the amino acid sequence set forth in SEQ ID NO:169, at least 50% sequence identity to the amino acid sequence set forth in SEQ ID NO:199, or at least 45% sequence identity to the amino acid sequence set forth in SEQ ID NO:201.

    38. An isolated nucleic acid molecule encoding a polypeptide capable of glycosylating a steviol precursor at its C-19 carboxyl or C-19 hydroxyl position or a catalytically active portion thereof, wherein the encoded polypeptide capable of glycosylating a steviol precursor at its C-19 carboxyl or C-19 hydroxyl position or the catalytically active portion thereof has at least 60% sequence identity to the amino acid sequence set forth in SEQ ID NO:127, at least 60% sequence identity to the amino acid sequence set forth in SEQ ID NO:133, at least 60% sequence identity to the amino acid sequence set forth in SEQ ID NO:135, at least 60% sequence identity to the amino acid sequence set forth in SEQ ID NO:137, at least 50% sequence identity to the amino acid sequence set forth in SEQ ID NO:141, at least 50% sequence identity to the amino acid sequence set forth in SEQ ID NO:145, at least 60% sequence identity to the amino acid sequence set forth in SEQ ID NO:147, at least 60% sequence identity to the amino acid sequence set forth in SEQ ID NO:153, at least 55% sequence identity to the amino acid sequence set forth in SEQ ID NO:177, at least 65% sequence identity to the amino acid sequence set forth in SEQ ID NO:181, at least 55% sequence identity to the amino acid sequence set forth in SEQ ID NO:183, at least 50% sequence identity to the amino acid sequence set forth in SEQ ID NO:185, at least 50% sequence identity to the amino acid sequence set forth in SEQ ID NO:203, at least 50% sequence identity to the amino acid sequence set forth in SEQ ID NO:205, at least 40% sequence identity to the amino acid sequence set forth in SEQ ID NO:207, or at least 55% sequence identity to the amino acid sequence set forth in SEQ ID NO:211.

    39. The isolated nucleic acid of any one of claims 35-38, wherein the nucleic acid is cDNA.

    Description

    BRIEF DESCRIPTION OF THE DRAWINGS

    [0127] The following detailed description of the embodiments of the present invention can be best understood when read in conjunction with the following drawings, where like structure is indicated with like reference numerals and in which:

    [0128] FIG. 1 shows representative primary steviol glycoside glycosylation reactions catalyzed by suitable uridine 5-diphospho (UDP) glycosyl transferases (UGT) enzymes and chemical structures for several of the compounds found in Stevia extracts.

    [0129] FIG. 2 shows the biochemical pathway for producing steviol from geranylgeranyl diphosphate using geranylgeranyl diphosphate synthase (GGPPS), ent-copalyl diphosphate synthase (CDPS), ent-kaurene synthase (KS), ent-kaurene oxidase (KO), and ent-kaurenoic acid hydroxylase (KAH) polypeptides.

    [0130] FIG. 3 shows the structures of steviol+6Glc (isomer 1) and steviol+7Glc (isomer 2).

    [0131] FIG. 4 shows the structures of steviol+4Glc (#26) and ent-kaurenoic Acid+3Glc (isomer 1).

    [0132] FIG. 5 shows the structures ent-kaurenoic acid+3Glc (isomer 2) and ent-kaurenol+3Glc (isomer 1).

    [0133] FIGS. 6A, 6B, and 6C show a .sup.1H NMR spectrum and .sup.1H and .sup.13C NMR chemical shifts (in ppm) for ent-kaurenoic acid+3Glc (isomer 1). FIGS. 6D, 6E, and 6F show a .sup.1H NMR spectrum and .sup.1H and .sup.13C NMR chemical shifts (in ppm) for ent-kaurenoic acid+3Glc (isomer 2). FIGS. 6G, 6H, and 6I show a .sup.1H NMR spectrum and .sup.1H and .sup.13C NMR chemical shifts (in ppm) for ent-kaurenol+3Glc (isomer 1). FIGS. 6J, 6K, 6L, and 6M show a .sup.1H NMR spectrum and .sup.1H and .sup.13C NMR chemical shifts (in ppm) for steviol+6Glc (isomer 1). FIGS. 6N, 6O, 6P, and 6Q show a .sup.1H NMR spectrum and .sup.1H and .sup.13C NMR chemical shifts (in ppm) for steviol+7Glc (isomer 2). FIGS. 6R, 6S, 6T, and 6U show a .sup.1H NMR spectrum and .sup.1H and .sup.13C NMR chemical shifts (in ppm) for steviol+4Glc (#26).

    [0134] Skilled artisans will appreciate that elements in the Figures are illustrated for simplicity and clarity and have not necessarily been drawn to scale. For example, the dimensions of some of the elements in the Figures can be exaggerated relative to other elements to help improve understanding of the embodiment(s) of the present invention.

    DETAILED DESCRIPTION OF THE INVENTION

    [0135] All publications, patents and patent applications cited herein are hereby expressly incorporated by reference for all purposes.

    [0136] Before describing the present invention in detail, a number of terms will be defined. As used herein, the singular forms a, an, and the include plural referents unless the context clearly dictates otherwise. For example, reference to a nucleic acid means one or more nucleic acids.

    [0137] It is noted that terms like preferably, commonly, and typically are not utilized herein to limit the scope of the claimed invention or to imply that certain features are critical, essential, or even important to the structure or function of the claimed invention. Rather, these terms are merely intended to highlight alternative or additional features that can or cannot be utilized in a particular embodiment of the present invention.

    [0138] For the purposes of describing and defining the present invention it is noted that the term substantially is utilized herein to represent the inherent degree of uncertainty that can be attributed to any quantitative comparison, value, measurement, or other representation. The term substantially is also utilized herein to represent the degree by which a quantitative representation can vary from a stated reference without resulting in a change in the basic function of the subject matter at issue.

    [0139] Methods well known to those skilled in the art can be used to construct genetic expression constructs and recombinant cells according to this invention. These methods include in vitro recombinant DNA techniques, synthetic techniques, in vivo recombination techniques, and polymerase chain reaction (PCR) techniques. See, for example, techniques as described in Green & Sambrook, 2012, MOLECULAR CLONING: A LABORATORY MANUAL, Fourth Edition, Cold Spring Harbor Laboratory, New York; Ausubel et al., 1989, CURRENT PROTOCOLS IN MOLECULAR BIOLOGY, Greene Publishing Associates and Wiley Interscience, New York, and PCR Protocols: A Guide to Methods and Applications (Innis et al., 1990, Academic Press, San Diego, Calif.).

    [0140] As used herein, the terms polynucleotide, nucleotide, oligonucleotide, and nucleic acid can be used interchangeably to refer to nucleic acid comprising DNA, RNA, derivatives thereof, or combinations thereof, in either single-stranded or double-stranded embodiments depending on context as understood by the skilled worker.

    [0141] As used herein, the terms microorganism, microorganism host, and microorganism host cell can be used interchangeably. As used herein, the terms recombinant host and recombinant host cell can be used interchangeably. The person of ordinary skill in the art will appreciate that the terms microorganism, microorganism host, and microorganism host cell, when used to describe a cell comprising a recombinant gene, may be taken to mean recombinant host or recombinant host cell. As used herein, the term recombinant host is intended to refer to a host, the genome of which has been augmented by at least one DNA sequence. Such DNA sequences include but are not limited to genes that are not naturally present, DNA sequences that are not normally transcribed into RNA or translated into a protein (expressed), and other genes or DNA sequences which one desires to introduce into a host. It will be appreciated that typically the genome of a recombinant host described herein is augmented through stable introduction of one or more recombinant genes. Generally, introduced DNA is not originally resident in the host that is the recipient of the DNA, but it is within the scope of this disclosure to isolate a DNA segment from a given host, and to subsequently introduce one or more additional copies of that DNA into the same host, e.g., to enhance production of the product of a gene or alter the expression pattern of a gene. In some instances, the introduced DNA will modify or even replace an endogenous gene or DNA sequence by, e.g., homologous recombination or site-directed mutagenesis. Suitable recombinant hosts include microorganisms.

    [0142] As used herein, the term recombinant gene refers to a gene or DNA sequence that is introduced into a recipient host, regardless of whether the same or a similar gene or DNA sequence may already be present in such a host. Introduced, or augmented in this context, is known in the art to mean introduced or augmented by the hand of man. Thus, a recombinant gene can be a DNA sequence from another species or can be a DNA sequence that originated from or is present in the same species but has been incorporated into a host by recombinant methods to form a recombinant host. It will be appreciated that a recombinant gene that is introduced into a host can be identical to a DNA sequence that is normally present in the host being transformed, and is introduced to provide one or more additional copies of the DNA to thereby permit overexpression or modified expression of the gene product of that DNA. In some aspects, said recombinant genes are encoded by cDNA. In other embodiments, recombinant genes are synthetic and/or codon-optimized for expression in S. cerevisiae.

    [0143] As used herein, the term engineered biosynthetic pathway refers to a biosynthetic pathway that occurs in a recombinant host, as described herein. In some aspects, one or more steps of the biosynthetic pathway do not naturally occur in an unmodified host. In some embodiments, a heterologous version of a gene is introduced into a host that comprises an endogenous version of the gene.

    [0144] As used herein, the term endogenous gene refers to a gene that originates from and is produced or synthesized within a particular organism, tissue, or cell. In some embodiments, the endogenous gene is a yeast gene. In some embodiments, the gene is endogenous to S. cerevisiae, including, but not limited to S. cerevisiae strain S288C. In some embodiments, an endogenous yeast gene is overexpressed. As used herein, the term overexpress is used to refer to the expression of a gene in an organism at levels higher than the level of gene expression in a wild type organism. See, e.g., Prelich, 2012, Genetics 190:841-54. In some embodiments, an endogenous yeast gene, for example ADH, is deleted. See, e.g., Giaever & Nislow, 2014, Genetics 197(2):451-65. As used herein, the terms deletion, deleted, knockout, and knocked out can be used interchangabley to refer to an endogenous gene that has been manipulated to no longer be expressed in an organism, including, but not limited to, S. cerevisiae.

    [0145] As used herein, the terms heterologous sequence and heterologous coding sequence are used to describe a sequence derived from a species other than the recombinant host. In some embodiments, the recombinant host is an S. cerevisiae cell, and a heterologous sequence is derived from an organism other than S. cerevisiae. A heterologous coding sequence, for example, can be from a prokaryotic microorganism, a eukaryotic microorganism, a plant, an animal, an insect, or a fungus different than the recombinant host expressing the heterologous sequence. In some embodiments, a coding sequence is a sequence that is native to the host.

    [0146] A selectable marker can be one of any number of genes that complement host cell auxotrophy, provide antibiotic resistance, or result in a color change. Linearized DNA fragments of the gene replacement vector then are introduced into the cells using methods well known in the art (see below). Integration of the linear fragments into the genome and the disruption of the gene can be determined based on the selection marker and can be verified by, for example, PCR or Southern blot analysis. Subsequent to its use in selection, a selectable marker can be removed from the genome of the host cell by, e.g., Cre-LoxP systems (see, e.g., Gossen et al., 2002, Ann. Rev. Genetics 36:153-173 and U.S. 2006/0014264). Alternatively, a gene replacement vector can be constructed in such a way as to include a portion of the gene to be disrupted, where the portion is devoid of any endogenous gene promoter sequence and encodes none, or an inactive fragment of, the coding sequence of the gene.

    [0147] As used herein, the terms variant and mutant are used to describe a protein sequence that has been modified at one or more amino acids, compared to the wild-type sequence of a particular protein.

    [0148] As used herein, the term inactive fragment is a fragment of the gene that encodes a protein having, e.g., less than about 10% (e.g., less than about 9%, less than about 8%, less than about 7%, less than about 6%, less than about 5%, less than about 4%, less than about 3%, less than about 2%, less than about 1%, or 0%) of the activity of the protein produced from the full-length coding sequence of the gene. Such a portion of a gene is inserted in a vector in such a way that no known promoter sequence is operably linked to the gene sequence, but that a stop codon and a transcription termination sequence are operably linked to the portion of the gene sequence. This vector can be subsequently linearized in the portion of the gene sequence and transformed into a cell. By way of single homologous recombination, this linearized vector is then integrated in the endogenous counterpart of the gene with inactivation thereof.

    [0149] As used herein, the term steviol glycoside refers to rebaudioside A (RebA) (CAS #58543-16-1), rebaudioside B (RebB) (CAS #58543-17-2), rebaudioside C (RebC) (CAS #63550-99-2), rebaudioside D (RebD) (CAS #63279-13-0), rebaudioside E (RebE) (CAS #63279-14-1), rebaudioside F (RebF) (CAS #438045-89-7), rebaudioside M (RebM) (CAS #1220616-44-3), rubusoside (CAS #63849-39-4), Dulcoside A (CAS #64432-06-0), rebaudioside I (RebI) (MassBank Record: FU000332), rebaudioside Q (RebQ), 1,2-stevioside (CAS #57817-89-7), 1,3-stevioside (RebG), steviol-1,2-bioside (MassBank Record: FU000299), steviol-1,3-bioside, steviol-13-O-glucoside (13-SMG), steviol-19-O-glucoside (19-SMG), a tri-glucosylated steviol glycoside, a tetra-glycosylated steviol glycoside, a penta-glucosylated steviol glycoside, a hexa-glucosylated steviol glycoside, a hepta-glucosylated steviol glycoside, and isomers thereof. See FIG. 1; see also, Steviol Glycosides Chemical and Technical Assessment 69th JECFA, 2007, prepared by Harriet Wallin, Food Agric. Org. Nuclear magnetic resonance (NMR) spectra for steviol glycoside isomers disclosed herein can be found in FIG. 6.

    [0150] As used herein, the terms steviol glycoside precursor and steviol glycoside precursor compound are used to refer to intermediate compounds in the steviol glycoside biosynthetic pathway. Steviol glycoside precursors include, but are not limited to, geranylgeranyl diphosphate (GGPP), ent-copalyl-diphosphate, ent-kaurene, ent-kaurenol, ent-kaurenal, ent-kaurenoic acid, and steviol. See FIG. 2. Also as used herein, the terms steviol precursor and steviol precursor compound are used to refer to intermediate compounds in the steviol biosynthetic pathway (i.e., compounds from which steviol may ultimately be synthesized). Steviol precursors include, but are not limited to, geranylgeranyl diphosphate (GGPP), ent-copalyl-diphosphate, ent-kaurene, ent-kaurenol, ent-kaurenal, and ent-kaurenoic acid. In some embodiments, steviol precurors can be glycosylated, e.g., tri-glycosylated ent-kaurenoic acid (ent-kaurenoic acid+3Glc), di-glycosylated ent-kaurenoic acid, mono-glycosylated ent-kaurenoic acid, tri-glycosylated ent-kaurenol, di-glycosylated ent-kaurenol (ent-kaurenol+2Glc), or mono-glycosylated ent-kaurenol (ent-kaurenol+1Glc). The person of ordinary skill in the art will appreciate that steviol precursors may be steviol glycoside precursors. In some embodiments, steviol glycoside precursors are themselves steviol glycoside compounds. For example, 19-SMG, rubusoside, stevioside, and RebE are steviol glycoside precursors of RebM. See FIG. 1.

    [0151] As used herein, the term contact is used to refer to any physical interaction between two objects. For example, the term contact may refer to the interaction between an an enzyme and a substrate. In another example, the term contact may refer to the interaction between a liquid (e.g., a supernatant) and an adsorbent resin.

    [0152] Steviol glycosides, steviol glycoside precursors, and/or glycosides of steviol precursors can be produced in vivo (i.e., in a recombinant host), in vitro (i.e., enzymatically), or by whole cell bioconversion. As used herein, the terms produce and accumulate can be used interchangeably to describe synthesis of steviol glycosides, glycosides of steviol precursors, and steviol glycoside precursors in vivo, in vitro, or by whole cell bioconversion.

    [0153] Recombinant steviol glycoside-producing Saccharomyces cerevisiae (S. cerevisiae) strains are described in WO 2011/153378, WO 2013/022989, WO 2014/122227, and WO 2014/122328. Methods of producing steviol glycosides in recombinant hosts, by whole cell bio-conversion, and in vitro are also described in WO 2011/153378, WO 2013/022989, WO 2014/122227, and WO 2014/122328.

    [0154] As used herein, the terms culture broth, culture medium, and growth medium can be used interchangeably to refer to a liquid or solid that supports growth of a cell. A culture broth can comprise glucose, fructose, sucrose, trace metals, vitamins, salts, yeast nitrogen base (YNB), and/or amino acids. The trace metals can be divalent cations, including, but not limited to, Mn.sup.2+ and/or Mg.sup.2+. In some embodiments, Mn.sup.2+ can be in the form of MnCl.sub.2 dihydrate and range from approximately 0.01 g/L to 100 g/L. In some embodiments, Mg.sup.2+ can be in the form of MgSO.sub.4 heptahydrate and range from approximately 0.01 g/L to 100 g/L. For example, a culture broth can comprise i) approximately 0.02-0.03 g/L MnCl.sub.2 dihydrate and approximately 0.5-3.8 g/L MgSO.sub.4 heptahydrate, ii) approximately 0.03-0.06 g/L MnCl.sub.2 dihydrate and approximately 0.5-3.8 g/L MgSO.sub.4 heptahydrate, and/or iii) approximately 0.03-0.17 g/L MnCl.sub.2 dihydrate and approximately 0.5-7.3 g/L MgSO.sub.4 heptahydrate. Additionally, a culture broth can comprise one or more steviol glycosides produced by a recombinant host, as described herein.

    [0155] In some embodiments, a recombinant host comprising a gene encoding a polypeptide capable of synthesizing geranylgeranyl pyrophosphate (GGPP) from farnesyl diphosphate (FPP) and isopentenyl diphosphate (IPP) (e.g., geranylgeranyl diphosphate synthase (GGPPS)); a gene encoding a polypeptide capable of synthesizing ent-copalyl diphosphate from GGPP (e.g., ent-copalyl diphosphate synthase (CDPS)); a gene encoding a polypeptide capable of synthesizing ent-kaurene from ent-copalyl diphosphate (e.g., kaurene synthase (KS)); a gene encoding a polypeptide capable of synthesizing ent-kaurenoic acid, ent-kaurenol, and/or ent-kaurenol from ent-kaurene (e.g., kaurene oxidase (KO)); a gene encoding a polypeptide capable of reducing cytochrome P450 complex (e.g., cytochrome P450 reductase (CPR) or P450 oxidoreductase (POR); for example, but not limited to a polypeptide capable of electron transfer from NADPH to cytochrome P450 complex during conversion of NADPH to NADP.sup.+, which is utilized as a cofactor for terpenoid biosynthesis); a gene encoding a polypeptide capable of synthesizing steviol from ent-kaurenoic acid (e.g., steviol synthase (KAH)); and/or a gene encoding a bifunctional polypeptide capable of synthesizing ent-copalyl diphosphate from GGPP and synthesizing ent-kaurene from ent-copalyl diphosphate (e.g., an ent-copalyl diphosphate synthase (CDPS)ent-kaurene synthase (KS) polypeptide) can produce steviol in vivo. See, e.g., FIG. 1. The skilled worker will appreciate that one or more of these genes can be endogenous to the host provided that at least one (and in some embodiments, all) of these genes is a recombinant gene introduced into the recombinant host.

    [0156] In some embodiments, a recombinant host comprising a gene encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-13 hydroxyl position (e.g., a UGT85C2 polypeptide); a gene encoding a polypeptide capable of beta 1,3 glycosylation of the C3 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside (e.g., a UGT76G1 polypeptide); a gene encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-19 carboxyl position (e.g., a UGT74G1 polypeptide); and/or a gene encoding a polypeptide capable of beta 1,2 glycosylation of the C2 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside (e.g., a UGT91D2 or EUGT11 polypeptide) can produce a steviol glycoside in vivo. The skilled worker will appreciate that one or more of these genes can be endogenous to the host provided that at least one (and in some embodiments, all) of these genes is a recombinant gene introduced into the recombinant host.

    [0157] In some embodiments, steviol glycosides, glycosides of steviol precursors, and/or steviol glycoside precursors are produced in vivo through expression of one or more enzymes involved in the steviol glycoside biosynthetic pathway in a recombinant host. For example, a recombinant host comprising a gene encoding a polypeptide capable of synthesizing GGPP from FPP and IPP; a gene encoding a polypeptide capable of synthesizing ent-copalyl diphosphate from GGPP; a gene encoding a polypeptide capable of synthesizing ent-kaurene from ent-copalyl diphosphate; a gene encoding a polypeptide capable of synthesizing ent-kaurenoic acid, ent-kaurenol, and/or ent-kaurenal from ent-kaurene; a gene encoding a polypeptide capable of reducing cytochrome P450 complex; a gene encoding a bifunctional polypeptide capable of synthesizing ent-copalyl diphosphate from GGPP and synthesizing ent-kaurene from ent-copalyl diphosphate; a gene encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-13 hydroxyl position; a gene encoding a polypeptide capable of beta 1,3 glycosylation of the C3 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside; a gene encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-19 carboxyl position; and/or a gene encoding a polypeptide capable of beta 1,2 glycosylation of the C2 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside can produce a steviol glycoside and/or steviol glycoside precursors in vivo. See, e.g., FIGS. 1 and 2. The skilled worker will appreciate that one or more of these genes can be endogenous to the host provided that at least one (and in some embodiments, all) of these genes is a recombinant gene introduced into the recombinant host.

    [0158] In some aspects, the polypeptide capable of synthesizing GGPP from FPP and IPP comprises a polypeptide having an amino acid sequence set forth in SEQ ID NO:20 (which can be encoded by the nucleotide sequence set forth in SEQ ID NO:19), SEQ ID NO:22 (encoded by the nucleotide sequence set forth in SEQ ID NO:21), SEQ ID NO:24 (encoded by the nucleotide sequence set forth in SEQ ID NO:23), SEQ ID NO:26 (encoded by the nucleotide sequence set forth in SEQ ID NO:25), SEQ ID NO:28 (encoded by the nucleotide sequence set forth in SEQ ID NO:27), SEQ ID NO:30 (encoded by the nucleotide sequence set forth in SEQ ID NO:29), SEQ ID NO:32 (encoded by the nucleotide sequence set forth in SEQ ID NO:31), or SEQ ID NO:116 (encoded by the nucleotide sequence set forth in SEQ ID NO:115).

    [0159] In some aspects, the polypeptide capable of synthesizing ent-copalyl diphosphate from GGPP comprises a polypeptide having an amino acid sequence set forth in SEQ ID NO:34 (which can be encoded by the nucleotide sequence set forth in SEQ ID NO:33), SEQ ID NO:36 (encoded by the nucleotide sequence set forth in SEQ ID NO:35), SEQ ID NO:38 (encoded by the nucleotide sequence set forth in SEQ ID NO:37), SEQ ID NO:40 (encoded by the nucleotide sequence set forth in SEQ ID NO:39), or SEQ ID NO:42 (encoded by the nucleotide sequence set forth in SEQ ID NO:41). In some embodiments, the polypeptide capable of synthesizing ent-copalyldiphosphate from GGPP lacks a chloroplast transit peptide.

    [0160] In some aspects, the polypeptide capable of synthesizing ent-kaurene from ent-copalyl pyrophosphate comprises a polypeptide having an amino acid sequence set forth in SEQ ID NO:44 (which can be encoded by the nucleotide sequence set forth in SEQ ID NO:43), SEQ ID NO:46 (encoded by the nucleotide sequence set forth in SEQ ID NO:45), SEQ ID NO:48 (encoded by the nucleotide sequence set forth in SEQ ID NO:47), SEQ ID NO:50 (encoded by the nucleotide sequence set forth in SEQ ID NO:49), or SEQ ID NO:52 (encoded by the nucleotide sequence set forth in SEQ ID NO:51).

    [0161] In some embodiments, a recombinant host comprises a gene encoding a bifunctional polypeptide capable of synthesizing ent-copalyl diphosphate from GGPP and synthesizing ent-kaurene from ent-copalyl pyrophosphate. In some aspects, the bifunctional polypeptide comprises a polypeptide having an amino acid sequence set forth in SEQ ID NO:54 (which can be encoded by the nucleotide sequence set forth in SEQ ID NO:53), SEQ ID NO:56 (encoded by the nucleotide sequence set forth in SEQ ID NO:55), or SEQ ID NO:58 (encoded by the nucleotide sequence set forth in SEQ ID NO:57).

    [0162] In some aspects, the polypeptide capable of synthesizing ent-kaurenoic acid, ent-kaurenol, and/or ent-kaurenol from ent-kaurene comprises a polypeptide having an amino acid sequence set forth in SEQ ID NO:60 (which can be encoded by the nucleotide sequence set forth in SEQ ID NO:59), SEQ ID NO:62 (encoded by the nucleotide sequence set forth in SEQ ID NO:61), SEQ ID NO:117 (encoded by the nucleotide sequence set forth in SEQ ID NO:63 or SEQ ID NO:64), SEQ ID NO:66 (encoded by the nucleotide sequence set forth in SEQ ID NO:65), SEQ ID NO:68 (encoded by the nucleotide sequence set forth in SEQ ID NO:67), SEQ ID NO:70 (encoded by the nucleotide sequence set forth in SEQ ID NO:69), SEQ ID NO:72 (encoded by the nucleotide sequence set forth in SEQ ID NO:71), SEQ ID NO:74 (encoded by the nucleotide sequence set forth in SEQ ID NO:73), or SEQ ID NO:76 (encoded by the nucleotide sequence set forth in SEQ ID NO:75).

    [0163] In some aspects, the polypeptide capable of reducing cytochrome P450 complex comprises a polypeptide having an amino acid sequence set forth in SEQ ID NO:78 (which can be encoded by the nucleotide sequence set forth in SEQ ID NO:77), SEQ ID NO:80 (encoded by the nucleotide sequence set forth in SEQ ID NO:79), SEQ ID NO:82 (encoded by the nucleotide sequence set forth in SEQ ID NO:81), SEQ ID NO:84 (encoded by the nucleotide sequence set forth in SEQ ID NO:83), SEQ ID NO:86 (encoded by the nucleotide sequence set forth in SEQ ID NO:85), SEQ ID NO:88 (encoded by the nucleotide sequence set forth in SEQ ID NO:87), SEQ ID NO:90 (encoded by the nucleotide sequence set forth in SEQ ID NO:89), or SEQ ID NO:92 (encoded by the nucleotide sequence set forth in SEQ ID NO:91).

    [0164] In some aspects, the polypeptide capable of synthesizing steviol from ent-kaurenoic acid comprises a polypeptide having an amino acid sequence set forth in SEQ ID NO:94 (which can be encoded by the nucleotide sequence set forth in SEQ ID NO:93), SEQ ID NO:97 (encoded by the nucleotide sequence set forth in SEQ ID NO:95 or SEQ ID NO:96), SEQ ID NO:100 (encoded by the nucleotide sequence set forth in SEQ ID NO:98 or SEQ ID NO:99), SEQ ID NO:101, SEQ ID NO:102, SEQ ID NO:103, SEQ ID NO:104, SEQ ID NO:106 (encoded by the nucleotide sequence set forth in SEQ ID NO:105), SEQ ID NO:108 (encoded by the nucleotide sequence set forth in SEQ ID NO:107), SEQ ID NO:110 (encoded by the nucleotide sequence set forth in SEQ ID NO:109), SEQ ID NO:112 (encoded by the nucleotide sequence set forth in SEQ ID NO:111), or SEQ ID NO:114 (encoded by the nucleotide sequence set forth in SEQ ID NO:113).

    [0165] In some embodiments, a recombinant host comprises a nucleic acid encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-13 hydroxyl position, a nucleic acid encoding a polypeptide capable of beta 1,3 glycosylation of the C3 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside, a nucleic acid encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-19 carboxyl position, a nucleic acid encoding a polypeptide capable of beta 1,2 glycosylation of the C2 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside. In certain such embodiments, the recombinant host further comprises a gene encoding a polypeptide capable of synthesizing GGPP from FPP and IPP; a gene encoding a polypeptide capable of synthesizing ent-copalyl diphosphate from GGPP; a gene encoding a polypeptide capable of synthesizing ent-kaurene from ent-copalyl diphosphate; a gene encoding a polypeptide capable of synthesizing ent-kaurenoic acid, ent-kaurenol, and/or ent-kaurenal from ent-kaurene; a gene encoding a polypeptide capable of reducing cytochrome P450 complex; and/or a gene encoding a bifunctional polypeptide capable of synthesizing ent-copalyl diphosphate from GGPP and synthesizing ent-kaurene from ent-copalyl diphosphate.

    [0166] In some embodiments, a recombinant host comprises a gene encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-19 carboxyl position, e.g., a UGT73C1 polypeptide, a UGT73C3 polypeptide, a UGT73C5 polypeptide, a UGT73C6 polypeptide, a UGT73E1 polypeptide, a UGT75B1 polypeptide, a UGT75L6 polypeptide, a Olel polypeptide, a UGT5 polypeptide, a SA Gtase polypeptide, a UDPG1 polypeptide, a UN1671 polypeptide, a UGT74F1 polypeptide, a UGT84B2 polypeptide, and/or a UGT74F2-like UGT polypeptide. In certain such embodiments, the recombinant host further comprises a gene encoding a polypeptide capable of synthesizing GGPP from FPP and IPP; a gene encoding a polypeptide capable of synthesizing ent-copalyl diphosphate from GGPP; a gene encoding a polypeptide capable of synthesizing ent-kaurene from ent-copalyl diphosphate; a gene encoding a polypeptide capable of synthesizing ent-kaurenoic acid, ent-kaurenol, and/or ent-kaurenal from ent-kaurene; a gene encoding a polypeptide capable of reducing cytochrome P450 complex; a gene encoding a bifunctional polypeptide capable of synthesizing ent-copalyl diphosphate from GGPP and synthesizing ent-kaurene from ent-copalyl diphosphate; a gene encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-13 hydroxyl position; a gene encoding a polypeptide capable of beta 1,3 glycosylation of the C3 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside; and/or a gene encoding a polypeptide capable of beta 1,2 glycosylation of the C2 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside.

    [0167] In some embodiments, a recombinant host comprises a gene encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-13 hydroxyl position, e.g., a UGT73C1 polypeptide, a UGT73C3 polypeptide, a UGT73C5 polypeptide, a UGT73C6 polypeptide, a UGT73C7 polypeptide, a UGT73E1 polypeptide, and/or a UGT76E12 polypeptide. In certain such embodiments, the recombinant host further comprises a gene encoding a polypeptide capable of synthesizing GGPP from FPP and IPP; a gene encoding a polypeptide capable of synthesizing ent-copalyl diphosphate from GGPP; a gene encoding a polypeptide capable of synthesizing ent-kaurene from ent-copalyl diphosphate; a gene encoding a polypeptide capable of synthesizing ent-kaurenoic acid, ent-kaurenol, and/or ent-kaurenal from ent-kaurene; a gene encoding a polypeptide capable of reducing cytochrome P450 complex; a gene encoding a bifunctional polypeptide capable of synthesizing ent-copalyl diphosphate from GGPP and synthesizing ent-kaurene from ent-copalyl diphosphate; a gene encoding a polypeptide capable of beta 1,3 glycosylation of the C3 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside; a gene encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-19 carboxyl position; and/or a gene encoding a polypeptide capable of beta 1,2 glycosylation of the C2 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside.

    [0168] In some embodiments, a recombinant host comprises a gene encoding a polypeptide capable of beta-1,2-glycosylation of the C2 and/or beta-1,3-glycosylation of the C3 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside (that is, examples of glycosyl-position glycosylation), e.g., a UGT73C6 polypeptide, a CaUGT3 polypeptide, a UN32491 polypeptide, and/or a UN1671 polypeptide. In certain such embodiments, the recombinant host further comprises a gene encoding a polypeptide capable of synthesizing GGPP from FPP and IPP; a gene encoding a polypeptide capable of synthesizing ent-copalyl diphosphate from GGPP; a gene encoding a polypeptide capable of synthesizing ent-kaurene from ent-copalyl diphosphate; a gene encoding a polypeptide capable of synthesizing ent-kaurenoic acid, ent-kaurenol, and/or ent-kaurenal from ent-kaurene; a gene encoding a polypeptide capable of reducing cytochrome P450 complex; a gene encoding a bifunctional polypeptide capable of synthesizing ent-copalyl diphosphate from GGPP and synthesizing ent-kaurene from ent-copalyl diphosphate; a gene encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-13 hydroxyl position; a gene encoding a polypeptide capable of beta 1,3 glycosylation of the C3 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside; a gene encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-19 carboxyl position; and/or a gene encoding a polypeptide capable of beta 1,2 glycosylation of the C2 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside.

    [0169] In some embodiments, a recombinant host comprises a gene encoding a polypeptide capable of glycosylating a steviol precursor at its C-19 carboxyl or C-19 hydroxyl position, e.g., a UGT73C1 polypeptide, a UGT73C3 polypeptide, a UGT73C5 polypeptide, a UGT73C6 polypeptide, a UGT73E1 polypeptide, a UGT75B1 polypeptide, a UGT75L6 polypeptide, a UGT76E12 polypeptide, a Olel polypeptide, a UGTS polypeptide, a SA Gtase, a UDPG1 polypeptide, a UGT74F1 polypeptide, a UGT75D1 polypeptide, a UGT84B2 polypeptide, and/or a UGT74F2-like UGT polypeptide. In certain such embodiments, the recombinant host further comprises a gene encoding a polypeptide capable of synthesizing GGPP from FPP and IPP; a gene encoding a polypeptide capable of synthesizing ent-copalyl diphosphate from GGPP; a gene encoding a polypeptide capable of synthesizing ent-kaurene from ent-copalyl diphosphate; a gene encoding a polypeptide capable of synthesizing ent-kaurenoic acid, ent-kaurenol, and/or ent-kaurenal from ent-kaurene; a gene encoding a polypeptide capable of reducing cytochrome P450 complex; a gene encoding a bifunctional polypeptide capable of synthesizing ent-copalyl diphosphate from GGPP and synthesizing ent-kaurene from ent-copalyl diphosphate; a gene encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-13 hydroxyl position; a gene encoding a polypeptide capable of beta 1,3 glycosylation of the C3 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside; a gene encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-19 carboxyl position; and/or a gene encoding a polypeptide capable of beta 1,2 glycosylation of the C2 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside.

    [0170] In some embodiments, a recombinant host comprises a nucleic acid encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-13 hydroxyl position (e.g., UGT85C2 polypeptide) (SEQ ID NO:7), a nucleic acid encoding a polypeptide capable of beta 1,3 glycosylation of the C3 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside (e.g., UGT76G1 polypeptide) (SEQ ID NO:9), a nucleic acid encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-19 carboxyl position (e.g., UGT74G1 polypeptide) (SEQ ID NO:4), a nucleic acid encoding a polypeptide capable of beta 1,2 glycosylation of the C2 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside (e.g., EUGT11 polypeptide) (SEQ ID NO:16). In some aspects, the polypeptide capable of beta 1,2 glycosylation of the C2 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside (e.g., UGT91D2 polypeptide) can be a UGT91D2e polypeptide (SEQ ID NO:11) or a UGT91D2e-b polypeptide (SEQ ID NO:13).

    [0171] In some aspects, the polypeptide capable of glycosylating steviol or a steviol glycoside at its C-13 hydroxyl position is encoded by the nucleotide sequence set forth in SEQ ID NO:5 or SEQ ID NO:6, the polypeptide capable of beta 1,3 glycosylation of the C3 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside is encoded by the nucleotide sequence set forth in SEQ ID NO:8, the polypeptide capable of glycosylating steviol or a steviol glycoside at its C-19 carboxyl position is encoded by the nucleotide sequence set forth in SEQ ID NO:3, the polypeptide capable of beta 1,2 glycosylation of the C2 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside is encoded by the nucleotide sequence set forth in SEQ ID NO:10,12,14 or 15. The skilled worker will appreciate that expression of these genes may be necessary to produce a particular steviol glycoside but that one or more of these genes can be endogenous to the host provided that at least one (and in some embodiments, all) of these genes is a recombinant gene introduced into the recombinant host.

    [0172] In a particular embodiment, a steviol-producing recombinant microorganism comprises exogenous nucleic acids encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-13 hydroxyl position, a polypeptide capable of beta 1,3 glycosylation of the C3 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside, and a polypeptide capable of beta 1,2 glycosylation of the C2 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside polypeptides.

    [0173] In another particular embodiment, a steviol-producing recombinant microorganism comprises exogenous nucleic acids encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-13 hydroxyl position; a polypeptide capable of beta 1,3 glycosylation of the C3 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside; a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-19 carboxyl position; and a polypeptide capable of beta 1,2 glycosylation of the C2 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside.

    [0174] In some embodiments, polypeptides capable of catalyzing the 19-O-glycosylation of ent-kaurenoic acid (KA) to ent-kaurenoic acid+1Glc (#58), in vitro, in a recombinant host, or by whole cell bioconversion include UGT73C1 (SEQ ID NO:127), UGT73C3 (SEQ ID NO:133), UGT73C5 (SEQ ID NO:135), UGT73C6 (SEQ ID NO:137), UGT73E1 (SEQ ID NO:141), UGT74G1 (SEQ ID NO:4), UGT75B1 (SEQ ID NO:145), UGT75L6 (SEQ ID NO:147), UGT76E12 (SEQ ID NO:153), Olel (SEQ ID NO:177), UGTS (SEQ ID NO:181), SA Gtase (SEQ ID NO:183), UDPG1 (SEQ ID NO:185), UGT74F1 (SEQ ID NO:203), UGT75D1 (SEQ ID NO:205), UGT84B2 (SEQ ID NO:207), CaUGT2 (SEQ ID NO:209), and a UGT74F2-like UGT polypeptide (SEQ ID NO:211). See, Example 3.

    [0175] In some embodiments, polypeptides capable of catalyzing the 13-O-glycosylation of steviol to 13-SMG, in vitro, in a recombinant host, or by whole cell bioconversion include UGT73C1 (SEQ ID NO:127), UGT73C3 (SEQ ID NO:133), UGT73C5 (SEQ ID NO:135), UGT73C6 (SEQ ID NO:137), UGT73C7 (SEQ ID NO:139), UGT73E1 (SEQ ID NO:141), UGT76E12 (SEQ ID NO:153), and UGT85C2 (SEQ ID NO:7). See, Example 3.

    [0176] In some embodiments, polypeptides capable of catalyzing the 19-O-glycosylation of steviol to 19-SMG, in vitro, in a recombinant host, or by whole cell bioconversion include UGT73C1 (SEQ ID NO:127), UGT73C3 (SEQ ID NO:133), UGT73C5 (SEQ ID NO:135), UGT73C6 (SEQ ID NO:137), UGT73E1 (SEQ ID NO:141), UGT74D1 (SEQ ID NO:143), UGT74G1 (SEQ ID NO:4), UGT75B1 (SEQ ID NO:145), UGT75L6 (SEQ ID NO:147), Olel (SEQ ID NO:177), UGT5 (SEQ ID NO:181), SA Gtase (SEQ ID NO:183), and UDPG1 (SEQ ID NO:185). See, Example 3.

    [0177] In some embodiments, polypeptides capable of catalyzing the 19-O-glycosylation of 13-SMG to rubusoside, in vitro, in a recombinant host, or by whole cell bioconversion include UGT73C1 (SEQ ID NO:127), UGT73C6 (SEQ ID NO:137), UGT74G1 (SEQ ID NO:4), UGT85C2 (SEQ ID NO:7), SA Gtase (SEQ ID NO:183), UDPG1 (SEQ ID NO:185), UN1671 (SEQ ID NO:201), UGT74F1 (SEQ ID NO:203), UGT75D1 (SEQ ID NO:205), UGT84B2 (SEQ ID NO:207), CaUGT2 (SEQ ID NO:209), and a UGT74F2-like UGT polypeptide (SEQ ID NO:211). See, Example 3.

    [0178] In some embodiments, polypeptides capable of catalyzing the glycosylation of 13-SMG (that is, an examples of glycosyl-position glycosylation) to steviol-1,2-bioside, in vitro, in a recombinant host, or by whole cell bioconversion include UGT91D2e-b (SEQ ID NO:13), EUGT11 (SEQ ID NO:16), and UN32491 (SEQ ID NO:199).

    [0179] In some embodiments, polypeptides capable of catalyzing the glycosyl-position glycosylation of rubusoside to 1,2-stevioside, in vitro, in a recombinant host, or by whole cell bioconversion include UGT73C6 (SEQ ID NO:137), UGT91D2e-b (SEQ ID NO:13), CaUGT3 (SEQ ID NO:169), and EUGT11 (SEQ ID NO:16). See, Example 3.

    [0180] In some embodiments, polypeptides capable of catalyzing the glycosyl-position glycosylation of rubusoside to steviol+3Glc (#55), in vitro, in a recombinant host, or by whole cell bioconversion include EUGT11 (SEQ ID NO:16).

    [0181] In some embodiments, polypeptides capable of catalyzing the 19-O-glycosylation of RebB to RebA, in vitro, in a recombinant host, or by whole cell bioconversion include UGT74G1 (SEQ ID NO:4). See, Example 3.

    [0182] In some embodiments, polypeptides capable of catalyzing the glycosyl-position glycosylation of RebA to RebD, in vitro, in a recombinant host, or by whole cell bioconversion include EUGT11 (SEQ ID NO:16).

    [0183] In some embodiments, polypeptides capable of catalyzing the glycosyl-position glycosylation of RebA to steviol+5Glc (#24), in vitro, in a recombinant host, or by whole cell bioconversion include EUGT11 (SEQ ID NO:16) and UN1671 (SEQ ID NO:201). See, Example 3.

    [0184] In some aspects, polypeptides capable of 19-O-glycosylation activity on steviol, steviol glycosides, and precurors thereof in vitro, in a recombinant host, or by whole cell bioconversion include UGT73C1 (SEQ ID NO:127), UGT73C3 (SEQ ID NO:133), UGT73C5 (SEQ ID NO:135), UGT73C6 (SEQ ID NO:137), UGT73E1 (SEQ ID NO:141), UGT74G1 (SEQ ID NO:4), UGT85C2 (SEQ ID NO:7), UGT75B1 (SEQ ID NO:145), UGT75L6 (SEQ ID NO:147), UGT76E12 (SEQ ID NO:153), Olel (SEQ ID NO:177), UGT5 (SEQ ID NO:181), SA Gtase (SEQ ID NO:183), UDPG1 (SEQ ID NO:185), UN1671 (SEQ ID NO:201), UGT74F1 (SEQ ID NO:203), UGT75D1 (SEQ ID NO:205), UGT84B2 (SEQ ID NO:207), and a UGT74F2-like UGT (SEQ ID NO:211). See, Example 3. Non-limiting examples of 19-O-glycosylation reactions include conversion of ent-kaurenoic acid to ent-kaurenoic acid+1Glc (#58), conversion of 13-SMG to rubusoside, and/or conversion of steviol to 19-SMG (see, e.g., FIG. 1).

    [0185] In some aspects, polypeptides capable of 13-O-glycosylation activity on steviol and steviol glycosides in vitro, in a recombinant host, or by whole cell bioconversion include UGT73C1 (SEQ ID NO:127), UGT73C3 (SEQ ID NO:133), UGT73C5 (SEQ ID NO:135), UGT73C6 (SEQ ID NO:137), UGT73C7 (SEQ ID NO:139), UGT73E1 (SEQ ID NO:141), UGT76E12 (SEQ ID NO:153), and UGT85C2 (SEQ ID NO:7). See, Example 3. A non-limiting example of a 13-O-glycosylation reaction includes conversion of steviol to 13-SMG (see, e.g., FIG. 1).

    [0186] In some aspects, polypeptides capable of glycosylation activity towards the glucose residues of steviol glycosides including, but not limited to, catalyzing the conversion of 13-SMG to steviol-1,2-bioside, catalyzing the conversion of rubusoside to 1,2-stevioside, and/or catalyzing the conversion of RebA to steviol+5Glc (#24) (see, e.g., FIG. 1), in vitro, in a recombinant host, or by whole cell bioconversion include UGT73C6 (SEQ ID NO:137), UGT91D2e-b (SEQ ID NO:13), CaUGT3 (SEQ ID NO:169), EUGT11 (SEQ ID NO:16), UN32491 (SEQ ID NO:199), and UN1671 (SEQ ID NO:201). See, Example 3.

    [0187] In some embodiments, a recombinant host comprises a nucleic acid encoding a UGT85C2 polypeptide (SEQ ID NO:7), a nucleic acid encoding a UGT76G1 polypeptide (SEQ ID NO:9), a nucleic acid encoding a UGT74G1 polypeptide (SEQ ID NO:4), a nucleic acid encoding a UGT91D2 polypeptide, and/or a nucleic acid encoding a EUGT11 polypeptide (SEQ ID NO:16). In some aspects, the UGT91D2 polypeptide can be a UGT91D2e polypeptide (SEQ ID NO:11) a UGT91D2e-b polypeptide (SEQ ID NO:13). In some embodiments, a recombinant host comprises a nucleic acid encoding a UGT73C1 polypeptide (SEQ ID NO:127), a nucleic acid encoding a UGT73C3 polypeptide (SEQ ID NO:133), a nucleic acid encoding a UGT73C5 polypeptide (SEQ ID NO:135), a nucleic acid encoding a UGT73C6 polypeptide (SEQ ID NO:137), a nucleic acid encoding a UGT73C7 polypeptide (SEQ ID NO:139), a nucleic acid encoding a UGT73E1 polypeptide (SEQ ID NO:141), a nucleic acid encoding a UGT74D1 polypeptide (SEQ ID NO:143), a nucleic acid encoding a UGT75B1 polypeptide (SEQ ID NO:145), a nucleic acid encoding a UGT75L6 polypeptide (SEQ ID NO:147), a nucleic acid encoding a UGT76E12 polypeptide (SEQ ID NO:153), a nucleic acid encoding a CaUGT3 polypeptide (SEQ ID NO:169), a nucleic acid encoding a Olel polypeptide (SEQ ID NO:177), a nucleic acid encoding a UGT5 (SEQ ID NO:181), a nucleic acid encoding a SA Gtase polypeptide (SEQ ID NO:183), a nucleic acid encoding a UDPG1 polypeptide (SEQ ID NO:185), a nucleic acid encoding a UN32491 polypeptide (SEQ ID NO:199), a nucleic acid encoding a UN1671 polypeptide (SEQ ID NO:201), a nucleic acid encoding a UGT74F1 polypeptide (SEQ ID NO:203), a nucleic acid encoding a UGT75D1 polypeptide (SEQ ID NO:205), a nucleic acid encoding a UGT84B2 polypeptide (SEQ ID NO:207), a nucleic acid encoding a CaUGT2 polypeptide (SEQ ID NO:209) or a nucleic acid encoding a UGT74F2-like UGT polypeptide (SEQ ID NO:211).

    [0188] In some aspects, the UGT85C2 polypeptide is encoded by the nucleotide sequence set forth in SEQ ID NO:5, SEQ ID NO:6 the UGT76G1 polypeptide is encoded by the nucleotide sequence set forth in SEQ ID NO:8, the UGT74G1 polypeptide is encoded by the nucleotide sequence set forth in SEQ ID NO:3 or SEQ ID NO:213, the UGT91D2e polypeptide is encoded by the nucleotide sequence set forth in SEQ ID NO:10, the UGT91D2e-b polypeptide is encoded by the nucleotide sequence set forth in SEQ ID NO:12 or SEQ ID NO:212, the EUGT11 polypeptide is encoded by the nucleotide sequence set forth in SEQ ID NO:14 or SEQ ID NO:15, the UGT73C1 polypeptide is encoded by the nucleotide sequence set forth in SEQ ID NO:126, the UGT73C3 polypeptide is encoded by the nucleotide sequence set forth in SEQ ID NO:132, the UGT73C5 polypeptide is encoded by the nucleotide sequence set forth in SEQ ID NO:134, the UGT73C6 polypeptide is encoded by the nucleotide sequence set forth in SEQ ID NO:136, the UGT73C7 polypeptide is encoded by the nucleotide sequence set forth in SEQ ID NO:138, the UGT73E1 polypeptide is encoded by the nucleotide sequence set forth in SEQ ID NO:140, the UGT74D1 polypeptide is encoded by the nucleotide sequence set forth in SEQ ID NO:142, the UGT75B1 polypeptide is encoded by the nucleotide sequence set forth in SEQ ID NO:144, the UGT75L6 polypeptide is encoded by the nucleotide sequence set forth in SEQ ID NO:146, the UGT76E12 polypeptide is encoded by the nucleotide sequence set forth in SEQ ID NO:152, the CaUGT3 polypeptide is encoded by the nucleotide sequence set forth in SEQ ID NO:168, the Olel polypeptide is encoded by the nucleotide sequence set forth in SEQ ID NO:176, the UGT5 polypeptide is encoded by the nucleotide sequence set forth in SEQ ID NO:180, the SA Gtase polypeptide is encoded by the nucleotide sequence set forth in SEQ ID NO:182, the UDPG1 polypeptide is encoded by the nucleotide sequence set forth in SEQ ID NO:184, the UN32491 polypeptide is encoded by the nucleotide sequence set forth in SEQ ID NO:198, the UN1671 polypeptide is encoded by the nucleotide sequence set forth in SEQ ID NO:200, the UGT74F1 polypeptide is encoded by the nucleotide sequence set forth in SEQ ID NO:202, the UGT75D1 polypeptide is encoded by the nucleotide sequence set forth in SEQ ID NO:204, the UGT84B2 polypeptide is encoded by the nucleotide sequence set forth in SEQ ID NO:206, the CaUGT2 polypeptide is encoded by the nucleotide sequence set forth in SEQ ID NO:208, and the UGT74F2-like UGT polypeptide is encoded by the nucleotide sequence set forth in SEQ ID NO:210.

    [0189] In some embodiments, steviol glycosides, glycosides of steviol precursors, and/or steviol glycoside precursors are produced through contact of a steviol glycoside precursor with one or more enzymes involved in the steviol glycoside pathway in vitro. For example, contacting steviol with one or more of a gene encoding a polypeptide capable of beta 1,3 glycosylation of the C3 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside, a polypeptide capable of beta 1,2 glycosylation of the C2 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside, and a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-13 hydroxyl position or a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-19 carboxyl position can result in production of a steviol glycoside in vitro. In some embodiments, a steviol glycoside precursor is produced through contact of an upstream steviol glycoside precursor with one or more enzymes involved in the steviol glycoside pathway in vitro. For example, contacting ent-kaurenoic acid with a polypeptide capable of synthesizing steviol from ent-kaurenoic acid can result in production of steviol in vitro.

    [0190] In some embodiments, one or more steviol glycosides and/or glycosylated steviol precursors, or a composition thereof are produced in vitro. In some embodiments the method comprises adding a UGT85C2 polypeptide having at least 55% identity to an amino acid sequence set forth in SEQ ID NO:7; a UGT76G1 polypeptide having at least 50% identity to an amino acid sequence set forth in SEQ ID NO:9; a UGT74G1 polypeptide having at least 55% identity to an amino acid sequence set forth in SEQ ID NO:4; a UGT91D2 functional homolog polypeptide comprising a UGT91D2e polypeptide having 90% or greater identity to an amino acid sequence set forth in SEQ ID NO:11 or a UGT91D2e-b polypeptide having 90% or greater identity to an amino acid sequence set forth in SEQ ID NO:13; a EUGT11 polypeptide having at least 65% identity to an amino acid sequence set forth in SEQ ID NO:16; a UGT73C1 polypeptide comprises a polypeptide having at least 60% identity to an amino acid sequence set forth in SEQ ID NO:127; a UGT73C3 polypeptide comprises a polypeptide having at least 60% identity to an amino acid sequence set forth in SEQ ID NO:133; a UGT73C5 polypeptide comprises a polypeptide having at least 60% identity to an amino acid sequence set forth in SEQ ID NO:135; a UGT73C6 polypeptide comprises a polypeptide having at least 60% identity to an amino acid sequence set forth in SEQ ID NO:137; a UGT73E1 polypeptide comprises a polypeptide having at least 50% identity to an amino acid sequence set forth in SEQ ID NO:141; a UGT75B1 polypeptide comprises a polypeptide having at least 50% sequence identity to an amino acid sequence set forth in SEQ ID NO:145; a UGT75L6 polypeptide comprises a polypeptide having at least 60% sequence identity to an amino acid sequence set forth in SEQ ID NO:147; a UGT76E12 polypeptide comprises a polypeptide having at least 60% sequence identity to an amino acid sequence set forth in SEQ ID NO:153; a Olel polypeptide comprises a polypeptide having at least 55% identity to an amino acid sequence set forth in SEQ ID NO:177; a UGTS polypeptide comprises a polypeptide having at least 65% identity to an amino acid sequence set forth in SEQ ID NO:181; a SA Gtase polypeptide comprises a polypeptide having at least 55% identity to an amino acid sequence set forth in SEQ ID NO:183; a UDPG1 polypeptide comprises a polypeptide having at least 50% sequence identity to an amino acid sequence set forth in SEQ ID NO:185; a UN1671 polypeptide comprises a polypeptide having at least 45% identity to an amino acid sequence set forth in SEQ ID NO:201; a UGT74F1 polypeptide comprises a polypeptide having at least 50% sequence identity to an amino acid sequence set forth in SEQ ID NO:203; a UGT75D1 polypeptide comprises a polypeptide having at least 50% sequence identity to an amino acid sequence set forth in SEQ ID NO:205; a UGT84B2 polypeptide comprises a polypeptide having at least 40% sequence identity to an amino acid sequence set forth in SEQ ID NO:207; a UGT74F2-like UGT polypeptide comprises a polypeptide having at least 55% identity to an amino acid sequence set forth in SEQ ID NO:211; a UGT73C7 polypeptide comprises a polypeptide having at least 60% identity to an amino acid sequence set forth in SEQ ID NO:139; a CaUGT3 polypeptide comprises a polypeptide having at least 50% identity to an amino acid sequence set forth in SEQ ID NO:169; and/or a UN32491 polypeptide comprises a polypeptide having at least 50% identity to an amino acid sequence set forth in SEQ ID NO:199; and a plant-derived or synthetic steviol glycoside precursor or a plant-derived or synthetic steviol to a reaction mixture; wherein at least one of the polypeptides is a recombinant polypeptide; and producing the one or more steviol glycosides and/or glycosylated steviol precursors, or the composition thereof, thereby.

    [0191] In some embodiments, a steviol glycoside or steviol glycoside precursor is produced by whole cell bioconversion. For whole cell bioconversion to occur, a host cell expressing one or more enzymes involved in the steviol glycoside pathway takes up and modifies the steviol glycoside or steviol glycoside precursor in the cell; following modification in vivo, the steviol glycoside or steviol glycoside precursor remains in the cell and/or is excreted into the cell culture medium. For example, a host cell expressing a gene encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-13 hydroxyl position; a gene encoding a polypeptide capable of beta 1,3 glycosylation of the C3 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside; a gene encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-19 carboxyl position; and/or a gene encoding a polypeptide capable of beta 1,2 glycosylation of the C2 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside can take up steviol and glycosylate steviol in the cell; following glycosylation in vivo, a steviol glycoside can be excreted into the culture medium. In certain such embodiments, the host cell may further express a gene encoding a polypeptide capable of synthesizing GGPP from FPP and IPP; a gene encoding a polypeptide capable of synthesizing ent-copalyl diphosphate from GGPP; a gene encoding a polypeptide capable of synthesizing ent-kaurene from ent-copalyl diphosphate; a gene encoding a polypeptide capable of synthesizing ent-kaurenoic acid, ent-kaurenol, and/or ent-kaurenal from ent-kaurene; a gene encoding a polypeptide capable of reducing cytochrome P450 complex; a gene encoding a polypeptide capable of synthesizing steviol from ent-kaurenoic acid; and/or a gene encoding a bifunctional polypeptide capable of synthesizing ent-copalyl diphosphate from GGPP and synthesizing ent-kaurene from ent-copalyl diphosphate.

    [0192] In some embodiments, the method for producing one or more steviol glycosides and/or glycosylated steviol precursors, or a composition thereof as disclosed herein comprises whole cell bioconversion of a plant-derived or synthetic steviol glycoside precursor or a plant-derived or synthetic steviol precursor in a cell culture medium of a recombinant host cell using (a) a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-19 carboxyl position; (b) a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-13 hydroxyl position; (c) a polypeptide capable of beta-1,2-glycosylation of the C2 and/or beta-1,3-glycosylation of the C3 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside (that is, examples of glycosyl-position glycosylation) activity on a steviol glycoside; and/or (d) a polypeptide is capable of glycosylating a steviol precursor at its C-19 carboxyl or C-19 hydroxyl position; wherein at least one of the polypeptide is a recombinant polypeptide expressed in the recombinant host cell, and producing the one or more steviol glycosides and/or glycosylated steviol precursors, or a composition thereof, thereby.

    [0193] In some embodiments of the method for producing one or more steviol glycosides and/or glycosylated steviol precursors, or a composition thereof as disclosed herein by whole cell bioconversion of a plant-derived or synthetic steviol glycoside precursor or a plant-derived or synthetic steviol precursor in a cell culture medium of a recombinant host cell described herein, the polypeptide capable of glycosylating steviol or a steviol glycoside at its C-19 carboxyl position comprises a UGT73C1 polypeptide, a UGT73C3 polypeptide, a UGT73C5 polypeptide, a UGT73C6 polypeptide, a UGT73E1 polypeptide, a UGT75B1 polypeptide, a UGT75L6 polypeptide, a Olel polypeptide, a UGT5 polypeptide, a SA Gtase polypeptide, a UDPG1 polypeptide, a UN1671 polypeptide, a UGT74F1 polypeptide, a UGT84B2 polypeptide, and/or a UGT74F2-like UGT polypeptide; the polypeptide capable of glycosylating steviol or a steviol glycoside at its C-13 hydroxyl position comprises a UGT73C1 polypeptide, a UGT73C3 polypeptide, a UGT73C5 polypeptide, a UGT73C6 polypeptide, a UGT73C7 polypeptide, a UGT73E1 polypeptide, and/or a UGT76E12 polypeptide; the polypeptide capable of beta-1,2-glycosylation of the C2 and/or beta-1,3-glycosylation of the C3 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside (that is, examples of glycosyl-position glycosylation) activity on a steviol glycoside comprises a UGT73C6 polypeptide, a CaUGT3 polypeptide, a UN32491 polypeptide, and/or a UN1671 polypeptide; and/or the polypeptide is capable of glycosylating a steviol precursor at its C-19 carboxyl or C-19 hydroxyl position comprises a UGT73C1 polypeptide, a UGT73C3 polypeptide, a UGT73C5 polypeptide, a UGT73C6 polypeptide, a UGT73E1 polypeptide, a UGT75B1 polypeptide, a UGT75L6 polypeptide, a UGT76E12 polypeptide, a Olel polypeptide, a UGT5 polypeptide, a SA Gtase, a UDPG1 polypeptide, a UGT74F1 polypeptide, a UGT75D1 polypeptide, a UGT84B2 polypeptide, and/or a UGT74F2-like UGT polypeptide.

    [0194] In some embodiments of the method for producing one or more steviol glycosides and/or glycosylated steviol precursors, or a composition thereof as disclosed herein by whole cell bioconversion of a plant-derived or synthetic steviol glycoside precursor or a plant-derived or synthetic steviol precursor in a cell culture medium of a recombinant host cell described herein, the UGT73C1 polypeptide comprises a polypeptide having at least 60% identity to an amino acid sequence set forth in SEQ ID NO:127, the UGT73C3 polypeptide comprises a polypeptide having at least 60% identity to an amino acid sequence set forth in SEQ ID NO:133, the UGT73C5 polypeptide comprises a polypeptide having at least 60% identity to an amino acid sequence set forth in SEQ ID NO:135, the UGT73C6 polypeptide comprises a polypeptide having at least 60% identity to an amino acid sequence set forth in SEQ ID NO:137, the UGT73E1 polypeptide comprises a polypeptide having at least 50% identity to an amino acid sequence set forth in SEQ ID NO:141, the UGT75B1 polypeptide comprises a polypeptide having at least 50% sequence identity to an amino acid sequence set forth in SEQ ID NO:145, the UGT75L6 polypeptide comprises a polypeptide having at least 60% sequence identity to an amino acid sequence set forth in SEQ ID NO:147, the UGT76E12 polypeptide comprises a polypeptide having at least 60% sequence identity to an amino acid sequence set forth in SEQ ID NO:153, the Olel polypeptide comprises a polypeptide having at least 55% identity to an amino acid sequence set forth in SEQ ID NO:177, the UGTS polypeptide comprises a polypeptide having at least 65% identity to an amino acid sequence set forth in SEQ ID NO:181, the SA Gtase polypeptide comprises a polypeptide having at least 55% identity to an amino acid sequence set forth in SEQ ID NO:183, the UDPG1 polypeptide comprises a polypeptide having at least 50% sequence identity to an amino acid sequence set forth in SEQ ID NO:185, the UN1671 polypeptide comprises a polypeptide having at least 45% identity to an amino acid sequence set forth in SEQ ID NO:201, the UGT74F1 polypeptide comprises a polypeptide having at least 50% sequence identity to an amino acid sequence set forth in SEQ ID NO:203, the UGT75D1 polypeptide comprises a polypeptide having at least 50% sequence identity to an amino acid sequence set forth in SEQ ID NO:205, the UGT84B2 polypeptide comprises a polypeptide having at least 40% sequence identity to an amino acid sequence set forth in SEQ ID NO:207, the UGT74F2-like UGT polypeptide comprises a polypeptide having at least 55% identity to an amino acid sequence set forth in SEQ ID NO:211, the UGT73C7 polypeptide comprises a polypeptide having at least 60% identity to an amino acid sequence set forth in SEQ ID NO:139, the CaUGT3 polypeptide comprises a polypeptide having at least 50% identity to an amino acid sequence set forth in SEQ ID NO:169, or the UN32491 polypeptide comprises a polypeptide having at least 50% identity to an amino acid sequence set forth in SEQ ID NO:199.

    [0195] In some embodiments, a polypeptide, e.g., a UGT polypeptide, can be displayed on the surface of the recombinant host cells disclosed herein by fusing it with anchoring motifs.

    [0196] In some embodiments, the cell is permeabilized to take up a substrate to be modified or to excrete a modified product. In some embodiments, a permeabilizing agent can be added to aid the feedstock entering into the host and product getting out. In some embodiments, the cells are permeabilized with a solvent such as toluene, or with a detergent such as Triton-X or Tween. In some embodiments, the cells are permeabilized with a surfactant, for example a cationic surfactant such as cetyltrimethylammonium bromide (CTAB). In some embodiments, the cells are permeabilized with periodic mechanical shock such as electroporation or a slight osmotic shock. For example, a crude lysate of the cultured microorganism can be centrifuged to obtain a supernatant. The resulting supernatant can then be applied to a chromatography column, e.g., a C18 column, and washed with water to remove hydrophilic compounds, followed by elution of the compound(s) of interest with a solvent such as methanol. The compound(s) can then be further purified by preparative HPLC. See also, WO 2009/140394.

    [0197] In some embodiments, steviol, one or more steviol glycoside precursors, and/or one or more steviol glycosides are produced by co-culturing of two or more hosts. In some embodiments, one or more hosts, each expressing one or more enzymes involved in the steviol glycoside pathway, produce steviol, one or more steviol glycoside precursors, and/or one or more steviol glycosides. For example, a host expressing a gene encoding a polypeptide capable of synthesizing GGPP from FPP and IPP; a gene encoding a polypeptide capable of synthesizing ent-copalyl diphosphate from GGPP; a gene encoding a polypeptide capable of synthesizing ent-kaurene from ent-copalyl diphosphate; a gene encoding a polypeptide capable of synthesizing ent-kaurenoic acid, ent-kaurenol, and/or ent-kaurenal from ent-kaurene; a gene encoding a polypeptide capable of reducing cytochrome P450 complex; a gene encoding a polypeptide capable of synthesizing steviol from ent-kaurenoic acid; and/or a gene encoding a bifunctional polypeptide capable of synthesizing ent-copalyl diphosphate from GGPP and synthesizing ent-kaurene from ent-copalyl diphosphate and a host expressing a gene encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-13 hydroxyl position; a gene encoding a polypeptide capable of beta 1,3 glycosylation of the C3 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside; a gene encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-19 carboxyl position; and/or a gene encoding a polypeptide capable of beta 1,2 glycosylation of the C2 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside, produce one or more steviol glycosides.

    [0198] In some embodiments, a recombinant host comprising a gene encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-19 carboxyl position, e.g., a UGT73C1 polypeptide, a UGT73C3 polypeptide, a UGT73C5 polypeptide, a UGT73C6 polypeptide, a UGT73E1 polypeptide, a UGT75B1 polypeptide, a UGT75L6 polypeptide, a Olel polypeptide, a UGT5 polypeptide, a SA Gtase polypeptide, a UDPG1 polypeptide, a UN1671 polypeptide, a UGT74F1 polypeptide, a UGT84B2 polypeptide, and/or a UGT74F2-like UGT polypeptide further comprises a gene encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-13 hydroxyl position (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:7); a gene encoding a polypeptide capable of beta 1,3 glycosylation of the C3 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:9); a gene encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-19 carboxyl position (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:4); and/or a gene encoding a polypeptide capable of beta 1,2 glycosylation of the C2 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:11, SEQ ID NO:13, or SEQ ID NO:16). In certain such embodiments, the recombinant host cell further comprises a gene encoding a polypeptide capable of synthesizing GGPP from FPP and IPP (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:20); a gene encoding a polypeptide capable of synthesizing ent-copalyl diphosphate from GGPP (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:40); a gene encoding a polypeptide capable of synthesizing ent-kaurene from ent-copalyl diphosphate (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:52); a gene encoding a polypeptide capable of synthesizing ent-kaurenoic acid, ent-kaurenol, and/or ent-kaurenal from ent-kaurene (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:60 or SEQ ID NO:117); a gene encoding a polypeptide capable of reducing cytochrome P450 complex (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:78, SEQ ID NO:86, or SEQ ID NO:92); and/or a gene encoding a polypeptide capable of synthesizing steviol from ent-kaurenoic acid (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:94).

    [0199] In some embodiments, a recombinant host comprising a gene encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-13 hydroxyl position, e.g., a UGT73C1 polypeptide, a UGT73C3 polypeptide, a UGT73C5 polypeptide, a UGT73C6 polypeptide, a UGT73C7 polypeptide, a UGT73E1 polypeptide, and/or a UGT76E12 polypeptide further comprises a gene encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-13 hydroxyl position (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:7); a gene encoding a polypeptide capable of beta 1,3 glycosylation of the C3 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:9); a gene encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-19 carboxyl position (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:4); and/or a gene encoding a polypeptide capable of beta 1,2 glycosylation of the C2 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:11, SEQ ID NO:13, or SEQ ID NO:16). In certain such embodiments, the recombinant host cell further comprises a gene encoding a polypeptide capable of synthesizing GGPP from FPP and IPP (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:20); a gene encoding a polypeptide capable of synthesizing ent-copalyl diphosphate from GGPP (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:40); a gene encoding a polypeptide capable of synthesizing ent-kaurene from ent-copalyl diphosphate (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:52); a gene encoding a polypeptide capable of synthesizing ent-kaurenoic acid, ent-kaurenol, and/or ent-kaurenal from ent-kaurene (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:60 or SEQ ID NO:117); a gene encoding a polypeptide capable of reducing cytochrome P450 complex (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:78, SEQ ID NO:86, or SEQ ID NO:92); and/or a gene encoding a polypeptide capable of synthesizing steviol from ent-kaurenoic acid (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:94).

    [0200] In some embodiments, a recombinant host comprising a gene encoding a polypeptide capable of beta-1,2-glycosylation of the C2 and/or beta-1,3-glycosylation of the C3 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside (that is, examples of glycosyl-position glycosylation), e.g., a UGT73C6 polypeptide, a CaUGT3 polypeptide, a UN32491 polypeptide, and/or a UN1671 polypeptide further comprises a gene encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-13 hydroxyl position (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:7); a gene encoding a polypeptide capable of beta 1,3 glycosylation of the C3 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:9); a gene encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-19 carboxyl position (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:4); and/or a gene encoding a polypeptide capable of beta 1,2 glycosylation of the C2 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:11, SEQ ID NO:13, or SEQ ID NO:16). In certain such embodiments, the recombinant host cell further comprises a gene encoding a polypeptide capable of synthesizing GGPP from FPP and IPP (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:20); a gene encoding a polypeptide capable of synthesizing ent-copalyl diphosphate from GGPP (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:40); a gene encoding a polypeptide capable of synthesizing ent-kaurene from ent-copalyl diphosphate (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:52); a gene encoding a polypeptide capable of synthesizing ent-kaurenoic acid, ent-kaurenol, and/or ent-kaurenal from ent-kaurene (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:60 or SEQ ID NO:117); a gene encoding a polypeptide capable of reducing cytochrome P450 complex (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:78, SEQ ID NO:86, or SEQ ID NO:92); and/or a gene encoding a polypeptide capable of synthesizing steviol from ent-kaurenoic acid (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:94).

    [0201] In some embodiments, a recombinant host comprising a gene encoding a polypeptide capable of glycosylating a steviol precursor at its C-19 carboxyl or C-19 hydroxyl position, e.g., a UGT73C1 polypeptide, a UGT73C3 polypeptide, a UGT73C5 polypeptide, a UGT73C6 polypeptide, a UGT73E1 polypeptide, a UGT75B1 polypeptide, a UGT75L6 polypeptide, a UGT76E12 polypeptide, a Olel polypeptide, a UGTS polypeptide, a SA Gtase, a UDPG1 polypeptide, a UGT74F1 polypeptide, a UGT75D1 polypeptide, a UGT84B2 polypeptide, and/or a UGT74F2-like UGT polypeptide further comprises a gene encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-13 hydroxyl position (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:7); a gene encoding a polypeptide capable of beta 1,3 glycosylation of the C3 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:9); a gene encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-19 carboxyl position (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:4); and/or a gene encoding a polypeptide capable of beta 1,2 glycosylation of the C2 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:11, SEQ ID NO:13, or SEQ ID NO:16). In certain such embodiments, the recombinant host cell further comprises a gene encoding a polypeptide capable of synthesizing GGPP from FPP and IPP (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:20); a gene encoding a polypeptide capable of synthesizing ent-copalyl diphosphate from GGPP (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:40); a gene encoding a polypeptide capable of synthesizing ent-kaurene from ent-copalyl diphosphate (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:52); a gene encoding a polypeptide capable of synthesizing ent-kaurenoic acid, ent-kaurenol, and/or ent-kaurenol from ent-kaurene (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:60 or SEQ ID NO:117); a gene encoding a polypeptide capable of reducing cytochrome P450 complex (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:78, SEQ ID NO:86, or SEQ ID NO:92); and/or a gene encoding a polypeptide capable of synthesizing steviol from ent-kaurenoic acid (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:94).

    [0202] In some embodiments, a recombinant host comprising a gene encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-19 carboxyl position, e.g., a SA Gtase (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:183) further comprises a gene encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-13 hydroxyl position (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:7); a gene encoding a polypeptide capable of beta 1,3 glycosylation of the C3 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:9); a gene encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-19 carboxyl position (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:4); and/or a gene encoding a polypeptide capable of beta 1,2 glycosylation of the C2 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:11, SEQ ID NO:13, or SEQ ID NO:16). In certain such embodiments, the recombinant host cell further comprises a gene encoding a polypeptide capable of synthesizing GGPP from FPP and IPP (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:20); a gene encoding a polypeptide capable of synthesizing ent-copalyl diphosphate from GGPP (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:40); a gene encoding a polypeptide capable of synthesizing ent-kaurene from ent-copalyl diphosphate (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:52); a gene encoding a polypeptide capable of synthesizing ent-kaurenoic acid, ent-kaurenol, and/or ent-kaurenal from ent-kaurene (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:60 or SEQ ID NO:117); a gene encoding a polypeptide capable of reducing cytochrome P450 complex (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:78, SEQ ID NO:86, or SEQ ID NO:92); and/or a gene encoding a polypeptide capable of synthesizing steviol from ent-kaurenoic acid (e.g., a polypeptide having the amino acid sequence set forth in SEQ ID NO:94).

    [0203] In some aspects, expression of SA Gtase (SEQ ID NO:182, SEQ ID NO:183) in S. cerevisiae comprising one or more copies of a recombinant gene encoding a GGPPS polypeptide (e.g., SEQ ID NO:19, SEQ ID NO:20), a recombinant gene encoding a truncated CDPS polypeptide (e.g., SEQ ID NO:39, SEQ ID NO:40), a recombinant gene encoding a KS polypeptide (e.g., SEQ ID NO:51, SEQ ID NO:52), a recombinant gene encoding a KO polypeptide (e.g., SEQ ID NO:59, SEQ ID NO:60), a recombinant gene encoding an ATR2 polypeptide (e.g., SEQ ID NO:91, SEQ ID NO:92), a recombinant gene encoding an EUGT11 polypeptide (e.g., SEQ ID NO:14/SEQ ID NO:15, SEQ ID NO:16), a recombinant gene encoding a KAH polypeptide (e.g., SEQ ID NO:93, SEQ ID NO:94), a recombinant gene encoding a CPR8 polypeptide (e.g., SEQ ID NO:85, SEQ ID NO:86), a recombinant gene encoding a UGT85C2 polypeptide (e.g., SEQ ID NO:5/SEQ ID NO:6/SEQ ID NO:149, SEQ ID NO:7) or a UGT85C2 variant (or functional homolog) of SEQ ID NO:7, a recombinant gene encoding a UGT74G1 polypeptide (e.g., SEQ ID NO:3, SEQ ID NO:4) of a UGT74G1 variant (or functional homolog) of SEQ ID NO:4, a recombinant gene encoding a UGT76G1 polypeptide (e.g., SEQ ID NO:8, SEQ ID NO:9) or a UGT76G1 variant (or functional homolog) of SEQ ID NO:9, and a recombinant gene encoding a UGT91D2e polypeptide (e.g., SEQ ID NO:10, SEQ ID NO:11) and/or a UGT91D2e variant (or functional homolog) of SEQ ID NO:11 such as a UGT91D2e-b (SEQ ID NO:12, SEQ ID NO:13) polypeptide results in increased ent-kaurenoic acid+2Glc (#7), ent-kaurenoic acid+3Glc (isomer 1), ent-kaurenoic acid+3Glc (isomer 2), 13-SMG, RebA, RebB, Steviol+4Glc (#36), Steviol+6Glc (isomer 1), Steviol+7Glc (isomer 2), and/or ent-Kaurenol+3Glc (isomer 1 and/or isomer 2). See, Example 4.

    [0204] In some embodiments, a steviol glycoside and/or glycoside of a steviol precursor, or a composition thereof produced in vivo, in vitro, or by whole cell bioconversion comprises fewer contaminants or less of any particular contaminant than a stevia extract from, inter alia, a stevia plant. Contaminants can include plant-derived compounds that contribute to off-flavors. Potential contaminants include pigments, lipids, proteins, phenolics, saccharides, spathulenol and other sesquiterpenes, labdane diterpenes, monoterpenes, decanoic acid, 8,11,14-eicosatrienoic acid, 2-methyloctadecane, pentacosane, octacosane, tetracosane, octadecanol, stigmasterol, -sitosterol, -amyrin, -amyrin, lupeol, -amryin acetate, pentacyclic triterpenes, centauredin, quercitin, epi-alpha-cadinol, carophyllenes and derivatives, beta-pinene, beta-sitosterol, and gibberellin.

    [0205] As used herein, the terms detectable amount, detectable concentration, measurable amount, and measurable concentration refer to a level of steviol glycosides measured in AUC, M/OD.sub.600, mg/L, M, or mM. Steviol glycoside production (i.e., total, supernatant, and/or intracellular steviol glycoside levels) can be detected and/or analyzed by techniques generally available to one skilled in the art, for example, but not limited to, liquid chromatography-mass spectrometry (LC-MS), thin layer chromatography (TLC), high-performance liquid chromatography (HPLC), ultraviolet-visible spectroscopy/spectrophotometry (UV-Vis), mass spectrometry (MS), and NMR.

    [0206] As used herein, the term undetectable concentration refers to a level of a compound that is too low to be measured and/or analyzed by techniques such as TLC, HPLC, UV-Vis, MS, or NMR. In some embodiments, a compound of an undetectable concentration is not present in a steviol glycoside or steviol glycoside precursor composition.

    [0207] As used herein, the terms or and and/or is utilized to describe multiple components in combination or exclusive of one another. For example, x, y, and/or z can refer to x alone, y alone, z alone, x, y, and z, (x and y) or z, x or (y and z), or x or y or z. In some embodiments, and/or is used to refer to the exogenous nucleic acids that a recombinant cell comprises, wherein a recombinant cell comprises one or more exogenous nucleic acids selected from a group. In some embodiments, and/or is used to refer to production of steviol glycosides and/or steviol glycoside precursors. In some embodiments, and/or is used to refer to production of steviol glycosides, wherein one or more steviol glycosides are produced. In some embodiments, and/or is used to refer to production of steviol glycosides, wherein one or more steviol glycosides are produced through one or more of the following steps: culturing a recombinant microorganism, synthesizing one or more steviol glycosides in a recombinant microorganism, and/or isolating one or more steviol glycosides.

    Functional Homologs

    [0208] Functional homologs of the polypeptides described above are also suitable for use in producing steviol glycosides in a recombinant host. A functional homolog is a polypeptide that has sequence similarity to a reference polypeptide, and that carries out one or more of the biochemical or physiological function(s) of the reference polypeptide. A functional homolog and the reference polypeptide can be a natural occurring polypeptide, and the sequence similarity can be due to convergent or divergent evolutionary events. As such, functional homologs are sometimes designated in the literature as homologs, or orthologs, or paralogs. Variants of a naturally occurring functional homolog, such as polypeptides encoded by mutants of a wild type coding sequence, can themselves be functional homologs. Functional homologs can also be created via site-directed mutagenesis of the coding sequence for a polypeptide, or by combining domains from the coding sequences for different naturally-occurring polypeptides (domain swapping). Techniques for modifying genes encoding functional polypeptides described herein are known and include, inter alia, directed evolution techniques, site-directed mutagenesis techniques and random mutagenesis techniques, and can be useful to increase specific activity of a polypeptide, alter substrate specificity, alter expression levels, alter subcellular location, or modify polypeptide-polypeptide interactions in a desired manner. Such modified polypeptides are considered functional homologs. The term functional homolog is sometimes applied to the nucleic acid that encodes a functionally homologous polypeptide.

    [0209] Functional homologs can be identified by analysis of nucleotide and polypeptide sequence alignments. For example, performing a query on a database of nucleotide or polypeptide sequences can identify homologs of steviol glycoside biosynthesis polypeptides. Sequence analysis can involve BLAST, Reciprocal BLAST, or PSI-BLAST analysis of non-redundant databases using a UGT amino acid sequence as the reference sequence. Amino acid sequence is, in some instances, deduced from the nucleotide sequence. Those polypeptides in the database that have greater than 40% sequence identity are candidates for further evaluation for suitability as a steviol glycoside biosynthesis polypeptide. Amino acid sequence similarity allows for conservative amino acid substitutions, such as substitution of one hydrophobic residue for another or substitution of one polar residue for another. If desired, manual inspection of such candidates can be carried out in order to narrow the number of candidates to be further evaluated. Manual inspection can be performed by selecting those candidates that appear to have domains present in steviol glycoside biosynthesis polypeptides, e.g., conserved functional domains. In some embodiments, nucleic acids and polypeptides are identified from transcriptome data based on expression levels rather than by using BLAST analysis.

    [0210] Conserved regions can be identified by locating a region within the primary amino acid sequence of a steviol glycoside biosynthesis polypeptide that is a repeated sequence, forms some secondary structure (e.g., helices and beta sheets), establishes positively or negatively charged domains, or represents a protein motif or domain. See, e.g., the Pfam web site describing consensus sequences for a variety of protein motifs and domains on the World Wide Web at sanger.ac.uk/Software/Pfam/ and pfam.janelia.org/. The information included at the Pfam database is described in Sonnhammer et al., Nucl. Acids Res., 26:320-322 (1998); Sonnhammer et al., Proteins, 28:405-420 (1997); and Bateman et al., Nucl. Acids Res., 27:260-262 (1999). Conserved regions also can be determined by aligning sequences of the same or related polypeptides from closely related species. Closely related species preferably are from the same family. In some embodiments, alignment of sequences from two different species is adequate to identify such homologs.

    [0211] Typically, polypeptides that exhibit at least about 40% amino acid sequence identity are useful to identify conserved regions. Conserved regions of related polypeptides exhibit at least 45% amino acid sequence identity (e.g., at least 50%, at least 60%, at least 70%, at least 80%, or at least 90% amino acid sequence identity). In some embodiments, a conserved region exhibits at least 92%, 94%, 96%, 98%, or 99% amino acid sequence identity.

    [0212] For example, polypeptides suitable for producing steviol in a recombinant host include functional homologs of UGTs.

    [0213] Methods to modify the substrate specificity of, for example, a UGT, are known to those skilled in the art, and include without limitation site-directed/rational mutagenesis approaches, random directed evolution approaches and combinations in which random mutagenesis/saturation techniques are performed near the active site of the enzyme. For example see Osmani et al., 2009, Phytochemistry 70: 325-347.

    [0214] A candidate sequence typically has a length that is from 80% to 200% of the length of the reference sequence, e.g., 82, 85, 87, 89, 90, 93, 95, 97, 99, 100, 105, 110, 115, 120, 130, 140, 150, 160, 170, 180, 190, or 200% of the length of the reference sequence. A functional homolog polypeptide typically has a length that is from 95% to 105% of the length of the reference sequence, e.g., 90, 93, 95, 97, 99, 100, 105, 110, 115, or 120% of the length of the reference sequence, or any range between. A % identity for any candidate nucleic acid or polypeptide relative to a reference nucleic acid or polypeptide can be determined as follows. A reference sequence (e.g., a nucleic acid sequence or an amino acid sequence described herein) is aligned to one or more candidate sequences using the computer program Clustal Omega (version 1.2.1, default parameters), which allows alignments of nucleic acid or polypeptide sequences to be carried out across their entire length (global alignment). Chenna et al., 2003, Nucleic Acids Res. 31(13):3497-500.

    [0215] ClustalW calculates the best match between a reference and one or more candidate sequences, and aligns them so that identities, similarities and differences can be determined. Gaps of one or more residues can be inserted into a reference sequence, a candidate sequence, or both, to maximize sequence alignments. For fast pairwise alignment of nucleic acid sequences, the following default parameters are used: word size: 2; window size: 4; scoring method: % age; number of top diagonals: 4; and gap penalty: 5. For multiple alignment of nucleic acid sequences, the following parameters are used: gap opening penalty: 10.0; gap extension penalty: 5.0; and weight transitions: yes. For fast pairwise alignment of protein sequences, the following parameters are used: word size: 1; window size: 5; scoring method: % age; number of top diagonals: 5; gap penalty: 3. For multiple alignment of protein sequences, the following parameters are used: weight matrix: blosum; gap opening penalty: 10.0; gap extension penalty: 0.05; hydrophilic gaps: on; hydrophilic residues: Gly, Pro, Ser, Asn, Asp, Gln, Glu, Arg, and Lys; residue-specific gap penalties: on. The ClustalW output is a sequence alignment that reflects the relationship between sequences. ClustalW can be run, for example, at the Baylor College of Medicine Search Launcher site on the World Wide Web (searchlauncher.bcm.tmc.edu/multi-align/multi-align.html) and at the European Bioinformatics Institute site on the World Wide Web (ebi.ac.uk/clustalw).

    [0216] To determine a % identity of a candidate nucleic acid or amino acid sequence to a reference sequence, the sequences are aligned using Clustal Omega, the number of identical matches in the alignment is divided by the length of the reference sequence, and the result is multiplied by 100. It is noted that the % identity value can be rounded to the nearest tenth. For example, 78.11, 78.12, 78.13, and 78.14 are rounded down to 78.1, while 78.15, 78.16, 78.17, 78.18, and 78.19 are rounded up to 78.2.

    [0217] It will be appreciated that functional UGT proteins can include additional amino acids that are not involved in the enzymatic activities carried out by the enzymes. In some embodiments, UGT proteins are fusion proteins. The terms chimera, fusion polypeptide, fusion protein, fusion enzyme, fusion construct, chimeric protein, chimeric polypeptide, chimeric construct, and chimeric enzyme can be used interchangeably herein to refer to proteins engineered through the joining of two or more genes that code for different proteins. In some embodiments, a nucleic acid sequence encoding a UGT polypeptide can include a tag sequence that encodes a tag designed to facilitate subsequent manipulation (e.g., to facilitate purification or detection), secretion, or localization of the encoded polypeptide. Tag sequences can be inserted in the nucleic acid sequence encoding the polypeptide such that the encoded tag is located at either the carboxyl or amino terminus of the polypeptide. Non-limiting examples of encoded tags include green fluorescent protein (GFP), human influenza hemagglutinin (HA), glutathione S transferase (GST), polyhistidine-tag (HIS tag), and Flag tag (Kodak, New Haven, Conn.). Other examples of tags include a chloroplast transit peptide, a mitochondrial transit peptide, an amyloplast peptide, signal peptide, or a secretion tag.

    [0218] In some embodiments, a fusion protein is a protein altered by domain swapping. As used herein, the term domain swapping is used to describe the process of replacing a domain of a first protein with a domain of a second protein. In some embodiments, the domain of the first protein and the domain of the second protein are functionally identical or functionally similar. In some embodiments, the structure and/or sequence of the domain of the second protein differs from the structure and/or sequence of the domain of the first protein. In some embodiments, a UGT polypeptide is altered by domain swapping.

    [0219] In some embodiments, a fusion protein is a protein altered by circular permutation, which consists in the covalent attachment of the ends of a protein that would be opened elsewhere afterwards. Thus, the order of the sequence is altered without causing changes in the amino acids of the protein. In some embodiments, a targeted circular permutation can be produced, for example but not limited to, by designing a spacer to join the ends of the original protein. Once the spacer has been defined, there are several possibilities to generate permutations through generally accepted molecular biology techniques, for example but not limited to, by producing concatemers by means of PCR and subsequent amplification of specific permutations inside the concatemer or by amplifying discrete fragments of the protein to exchange to join them in a different order. The step of generating permutations can be followed by creating a circular gene by binding the fragment ends and cutting back at random, thus forming collections of permutations from a unique construct.

    Steviol and Steviol Glycoside Biosynthesis Nucleic Acids

    [0220] A recombinant gene encoding a polypeptide described herein comprises the coding sequence for that polypeptide, operably linked in sense orientation to one or more regulatory regions suitable for expressing the polypeptide. Because many microorganisms are capable of expressing multiple gene products from a polycistronic mRNA, multiple polypeptides can be expressed under the control of a single regulatory region for those microorganisms, if desired. A coding sequence and a regulatory region are considered to be operably linked when the regulatory region and coding sequence are positioned so that the regulatory region is effective for regulating transcription or translation of the sequence. Typically, the translation initiation site of the translational reading frame of the coding sequence is positioned between one and about fifty nucleotides downstream of the regulatory region for a monocistronic gene.

    [0221] In many cases, the coding sequence for a polypeptide described herein is identified in a species other than the recombinant host, i.e., is a heterologous nucleic acid. Thus, if the recombinant host is a microorganism, the coding sequence can be from other prokaryotic or eukaryotic microorganisms, from plants or from animals. In some case, however, the coding sequence is a sequence that is native to the host and is being reintroduced into that organism.

    [0222] A native sequence can often be distinguished from the naturally occurring sequence by the presence of non-natural sequences linked to the exogenous nucleic acid, e.g., non-native regulatory sequences flanking a native sequence in a recombinant nucleic acid construct. In addition, stably transformed exogenous nucleic acids typically are integrated at positions other than the position where the native sequence is found. Regulatory region refers to a nucleic acid having nucleotide sequences that influence transcription or translation initiation and rate, and stability and/or mobility of a transcription or translation product. Regulatory regions include, without limitation, promoter sequences, enhancer sequences, response elements, protein recognition sites, inducible elements, protein binding sequences, 5 and 3 untranslated regions (UTRs), transcriptional start sites, termination sequences, polyadenylation sequences, introns, and combinations thereof. A regulatory region typically comprises at least a core (basal) promoter. A regulatory region also may include at least one control element, such as an enhancer sequence, an upstream element or an upstream activation region (UAR). A regulatory region is operably linked to a coding sequence by positioning the regulatory region and the coding sequence so that the regulatory region is effective for regulating transcription or translation of the sequence. For example, to operably link a coding sequence and a promoter sequence, the translation initiation site of the translational reading frame of the coding sequence is typically positioned between one and about fifty nucleotides downstream of the promoter. A regulatory region can, however, be positioned as much as about 5,000 nucleotides upstream of the translation initiation site, or about 2,000 nucleotides upstream of the transcription start site.

    [0223] The choice of regulatory regions to be included depends upon several factors, including, but not limited to, efficiency, selectability, inducibility, desired expression level, and preferential expression during certain culture stages. It is a routine matter for one of skill in the art to modulate the expression of a coding sequence by appropriately selecting and positioning regulatory regions relative to the coding sequence. It will be understood that more than one regulatory region may be present, e.g., introns, enhancers, upstream activation regions, transcription terminators, and inducible elements.

    [0224] One or more genes can be combined in a recombinant nucleic acid construct in modules useful for a discrete aspect of steviol and/or steviol glycoside production. Combining a plurality of genes in a module, particularly a polycistronic module, facilitates the use of the module in a variety of species. For example, a steviol biosynthesis gene cluster, or a UGT gene cluster, can be combined in a polycistronic module such that, after insertion of a suitable regulatory region, the module can be introduced into a wide variety of species. As another example, a UGT gene cluster can be combined such that each UGT coding sequence is operably linked to a separate regulatory region, to form a UGT module. Such a module can be used in those species for which monocistronic expression is necessary or desirable. In addition to genes useful for steviol or steviol glycoside production, a recombinant construct typically also contains an origin of replication, and one or more selectable markers for maintenance of the construct in appropriate species.

    [0225] It will be appreciated that because of the degeneracy of the genetic code, a number of nucleic acids can encode a particular polypeptide; i.e., for many amino acids, there is more than one nucleotide triplet that serves as the codon for the amino acid. Thus, codons in the coding sequence for a given polypeptide can be modified such that optimal expression in a particular host is obtained, using appropriate codon bias tables for that host (e.g., microorganism). As isolated nucleic acids, these modified sequences can exist as purified molecules and can be incorporated into a vector or a virus for use in constructing modules for recombinant nucleic acid constructs.

    [0226] In some cases, it is desirable to inhibit one or more functions of an endogenous polypeptide in order to divert metabolic intermediates towards steviol or steviol glycoside biosynthesis. For example, it may be desirable to downregulate synthesis of sterols in a yeast strain in order to further increase steviol or steviol glycoside production, e.g., by downregulating squalene epoxidase. As another example, it may be desirable to inhibit degradative functions of certain endogenous gene products, e.g., glycohydrolases that remove glucose moieties from secondary metabolites or phosphatases as discussed herein. In such cases, a nucleic acid that overexpresses the polypeptide or gene product may be included in a recombinant construct that is transformed into the strain. Alternatively, mutagenesis can be used to generate mutants in genes for which it is desired to increase or enhance function.

    [0227] One aspect of the disclosure is an isolated nucleic acid molecule encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-19 carboxyl position or a catalytically active portion thereof. The nucleic acid is cDNA. In some embodiments, the encoded polypeptide capable of glycosylating steviol or a steviol glycoside at its C-19 carboxyl position or the catalytically active portion thereof comprises a a UGT73C1 polypeptide, a UGT73C3 polypeptide, a UGT73C5 polypeptide, a UGT73C6 polypeptide, a UGT73E1 polypeptide, a UGT75B1 polypeptide, a UGT75L6 polypeptide, a Olel polypeptide, a UGT5 polypeptide, a SA Gtase polypeptide, a UDPG1 polypeptide, a UN1671 polypeptide, a UGT74F1 polypeptide, a UGT84B2 polypeptide, or a UGT74F2-like UGT polypeptide. In some embodiments, the encoded polypeptide capable of glycosylating steviol or a steviol glycoside at its C-19 carboxyl position or the catalytically active portion thereof comprises a polypeptide having the amino acid sequence set forth in SEQ ID NO:127, SEQ ID NO:133, SEQ ID NO:135, SEQ ID NO:137, SEQ ID NO:141, SEQ ID NO:145, SEQ ID NO:147, SEQ ID NO:177, SEQ ID NO:181, SEQ ID NO:183, SEQ ID NO:185, SEQ ID NO:201, SEQ ID NO:203, SEQ ID NO:207, or SEQ ID NO:211.

    [0228] Another aspect of the disclosure is an isolated nucleic acid molecule encoding a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-13 hydroxyl position or a catalytically active portion thereof. In some embodiments, the encoded polypeptide capable of glycosylating steviol or a steviol glycoside at its C-13 hydroxyl position or the catalytically active portion thereof comprises a UGT73C1 polypeptide, a UGT73C3 polypeptide, a UGT73C5 polypeptide, a UGT73C6 polypeptide, a UGT73C7 polypeptide, a UGT73E1 polypeptide, or a UGT76E12 polypeptide. In some embodiments, the encoded polypeptide capable of glycosylating steviol or a steviol glycoside at its C-13 hydroxyl position or the catalytically active portion thereof comprises a polypeptide having the amino acid sequence set forth in SEQ ID NO:127, SEQ ID NO:133, SEQ ID NO:135, SEQ ID NO:137, SEQ ID NO:139, SEQ ID NO:141, or SEQ ID NO:153.

    [0229] Another aspect of the disclosure is an isolated nucleic acid molecule encoding a polypeptide capable of beta-1,2-glycosylation of the C2 and/or beta-1,3-glycosylation of the C3 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside or a catalytically active portion thereof. The nucleic acid is cDNA. In some embodiments, the encoded polypeptide capable of beta-1,2-glycosylation of the C2 and/or beta-1,3-glycosylation of the C3 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside or the catalytically active portion thereof comprises a UGT73C6 polypeptide, a CaUGT3 polypeptide, a UN32491 polypeptide, or a UN1671 polypeptide. In some embodiments, the encoded polypeptide capable of beta-1,2-glycosylation of the C2 and/or beta-1,3-glycosylation of the C3 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside or the catalytically active portion thereof comprises a polypeptide having the amino acid sequence set forth in SEQ ID NO: 137, SEQ ID NO:169, SEQ ID NO:199, or SEQ ID NO:201.

    [0230] Another aspect of the disclosure is an isolated nucleic acid molecule encoding a polypeptide capable of glycosylating a steviol precursor at its C-19 carboxyl or C-19 hydroxyl position or a catalytically active portion thereof. The nucleic acid is cDNA. In some embodiments, the encoded polypeptide capable of glycosylating a steviol precursor at its C-19 carboxyl or C-19 hydroxyl position or the catalytically active portion thereof comprises a UGT73C1 polypeptide, a UGT73C3 polypeptide, a UGT73C5 polypeptide, a UGT73C6 polypeptide, a UGT73E1 polypeptide, a UGT75B1 polypeptide, a UGT75L6 polypeptide, a UGT76E12 polypeptide, a Olel polypeptide, a UGT5 polypeptide, a SA Gtase, a UDPG1 polypeptide, a UGT74F1 polypeptide, a UGT75D1 polypeptide, a UGT84B2 polypeptide, or a UGT74F2-like UGT polypeptide. In some embodiments, the encoded polypeptide capable of glycosylating a steviol precursor at its C-19 carboxyl or C-19 hydroxyl position or the catalytically active portion thereof comprises a polypeptide having the amino acid sequence set forth in SEQ ID NO: 127, SEQ ID NO:133, SEQ ID NO:135, SEQ ID NO:137, SEQ ID NO:141, SEQ ID NO:145, SEQ ID NO:147, SEQ ID NO:153, SEQ ID NO:177, SEQ ID NO:181, SEQ ID NO:183, SEQ ID NO:185, SEQ ID NO:203, SEQ ID NO:205, SEQ ID NO:207, or SEQ ID NO:211.

    Host Microorganisms

    [0231] Recombinant hosts can be used to express polypeptides for the producing steviol glycosides, including mammalian, insect, plant, and algal cells. A number of prokaryotes and eukaryotes are also suitable for use in constructing the recombinant microorganisms described herein, e.g., gram-negative bacteria, yeast, and fungi. A species and strain selected for use as a steviol glycoside production strain is first analyzed to determine which production genes are endogenous to the strain and which genes are not present. Genes for which an endogenous counterpart is not present in the strain are advantageously assembled in one or more recombinant constructs, which are then transformed into the strain in order to supply the missing function(s).

    [0232] Typically, the recombinant microorganism is grown in a fermenter at a temperature(s) for a period of time, wherein the temperature and period of time facilitate the production of a steviol glycoside. The constructed and genetically engineered microorganisms provided by the invention can be cultivated using conventional fermentation processes, including, inter alia, chemostat, batch, fed-batch cultivations, semi-continuous fermentations such as draw and fill, continuous perfusion fermentation, and continuous perfusion cell culture. Depending on the particular microorganism used in the method, other recombinant genes such as isopentenyl biosynthesis genes and terpene synthase and cyclase genes may also be present and expressed. Levels of substrates and intermediates, e.g., isopentenyl diphosphate, dimethylallyl diphosphate, GGPP, ent-kaurene and ent-kaurenoic acid, can be determined by extracting samples from culture media for analysis according to published methods.

    [0233] Carbon sources of use in the instant method include any molecule that can be metabolized by the recombinant host cell to facilitate growth and/or production of the steviol glycosides. Examples of suitable carbon sources include, but are not limited to, sucrose (e.g., as found in molasses), fructose, xylose, ethanol, glycerol, glucose, cellulose, starch, cellobiose or other glucose-comprising polymer. In embodiments employing yeast as a host, for example, carbons sources such as sucrose, fructose, xylose, ethanol, glycerol, and glucose are suitable. The carbon source can be provided to the host organism throughout the cultivation period or alternatively, the organism can be grown for a period of time in the presence of another energy source, e.g., protein, and then provided with a source of carbon only during the fed-batch phase.

    [0234] After the recombinant microorganism has been grown in culture for the period of time, wherein the temperature and period of time facilitate the production of a steviol glycoside, steviol and/or one or more steviol glycosides can then be recovered from the culture using various techniques known in the art. In some embodiments, a permeabilizing agent can be added to aid the feedstock entering into the host and product getting out. For example, a crude lysate of the cultured microorganism can be centrifuged to obtain a supernatant. The resulting supernatant can then be applied to a chromatography column, e.g., a C-18 column, and washed with water to remove hydrophilic compounds, followed by elution of the compound(s) of interest with a solvent such as methanol. The compound(s) can then be further purified by preparative HPLC. See also, WO 2009/140394.

    [0235] It will be appreciated that the various genes and modules discussed herein can be present in two or more recombinant hosts rather than a single host. When a plurality of recombinant hosts is used, they can be grown in a mixed culture to accumulate steviol and/or steviol glycosides.

    [0236] Alternatively, the two or more hosts each can be grown in a separate culture medium and the product of the first culture medium, e.g., steviol, can be introduced into second culture medium to be converted into a subsequent intermediate, or into an end product such as, for example, RebA. The product produced by the second, or final host is then recovered. It will also be appreciated that in some embodiments, a recombinant host is grown using nutrient sources other than a culture medium and utilizing a system other than a fermenter.

    [0237] Exemplary prokaryotic and eukaryotic species are described in more detail below. However, it will be appreciated that other species can be suitable. For example, suitable species can be in a genus such as Agaricus, Aspergillus, Bacillus, Candida, Corynebacterium, Eremothecium, Escherichia, Fusarium/Gibberella, Kluyveromyces, Laetiporus, Lentinus, Phaffia, Phanerochaete, Pichia, Physcomitrella, Rhodoturula, Saccharomyces, Schizosaccharomyces, Sphaceloma, Xanthophyllomyces or Yarrowia. Exemplary species from such genera include Lentinus tigrinus, Laetiporus sulphureus, Phanerochaete chrysosporium, Pichia pastoris, Cyberlindnera jadinii, Physcomitrella patens, Rhodoturula glutinis, Rhodoturula mucilaginosa, Phaffia rhodozyma, Xanthophyllomyces dendrorhous, Fusarium fujikuroi/Gibberella fujikuroi, Candida utilis, Candida glabrata, Candida albicans, and Yarrowia lipolytica.

    [0238] In some embodiments, a microorganism can be a prokaryote such as Escherichia bacteria cells, for example, Escherichia coli cells; Lactobacillus bacteria cells; Lactococcus bacteria cells; Comebacterium bacteria cells; Acetobacter bacteria cells; Acinetobacter bacteria cells; or Pseudomonas bacterial cells.

    [0239] In some embodiments, a microorganism can be an Ascomycete such as Gibberella fujikuroi, Kluyveromyces lactis, Schizosaccharomyces pombe, Aspergillus niger, Yarrowia lipolytica, Ashbya gossypii, or S. cerevisiae.

    [0240] In some embodiments, a microorganism can be an algal cell such as Blakeslea trispora, Dunaliella salina, Haematococcus pluvialis, Chlorella sp., Undaria pinnatifida, Sargassum, Laminaria japonica, Scenedesmus almeriensis species.

    [0241] In some embodiments, a microorganism can be a cyanobacterial cell such as Blakeslea trispora, Dunaliella salina, Haematococcus pluvialis, Chlorefia sp., Undaria pinnatifida, Sargassum, Laminaria japonica, Scenedesmus almeriensis.

    Saccharomyces spp.

    [0242] Saccharomyces is a widely used chassis organism in synthetic biology, and can be used as the recombinant microorganism platform. For example, there are libraries of mutants, plasmids, detailed computer models of metabolism and other information available for S. cerevisiae, allowing for rational design of various modules to enhance product yield. Methods are known for making recombinant microorganisms.

    Aspergillus spp.

    [0243] Aspergillus species such as A. oryzae, A. niger and A. sojae are widely used microorganisms in food production and can also be used as the recombinant microorganism platform. Nucleotide sequences are available for genomes of A. nidulans, A. fumigatus, A. oryzae, A. clavatus, A. flavus, A. niger, and A. terreus, allowing rational design and modification of endogenous pathways to enhance flux and increase product yield. Metabolic models have been developed for Aspergillus, as well as transcriptomic studies and proteomics studies. A. niger is cultured for the industrial production of a number of food ingredients such as citric acid and gluconic acid, and thus species such as A. niger are generally suitable for producing steviol glycosides.

    E. coli

    [0244] E. coli, another widely used platform organism in synthetic biology, can also be used as the recombinant microorganism platform. Similar to Saccharomyces, there are libraries of mutants, plasmids, detailed computer models of metabolism and other information available for E. coli, allowing for rational design of various modules to enhance product yield. Methods similar to those described above for Saccharomyces can be used to make recombinant E. coli microorganisms.

    Agaricus, Gibberella, and Phanerochaete spp.

    [0245] Agaricus, Gibberella, and Phanerochaete spp. can be useful because they are known to produce large amounts of isoprenoids in culture. Thus, the terpene precursors for producing large amounts of steviol glycosides are already produced by endogenous genes. Thus, modules comprising recombinant genes for steviol glycoside biosynthesis polypeptides can be introduced into species from such genera without the necessity of introducing mevalonate or MEP pathway genes.

    Arxula adeninivorans (Blastobotrys adeninivorans)

    [0246] Arxula adeninivorans is dimorphic yeast (it grows as budding yeast like the baker's yeast up to a temperature of 42 C., above this threshold it grows in a filamentous form) with unusual biochemical characteristics. It can grow on a wide range of substrates and can assimilate nitrate. It has successfully been applied to the generation of strains that can produce natural plastics or the development of a biosensor for estrogens in environmental samples.

    Yarrowia lipolytica

    [0247] Yarrowia lipolytica is dimorphic yeast (see Arxula adeninivorans) and belongs to the family Hemiascomycetes. The entire genome of Yarrowia lipolytica is known. Yarrowia species is aerobic and considered to be non-pathogenic. Yarrowia is efficient in using hydrophobic substrates (e.g. alkanes, fatty acids, oils) and can grow on sugars. It has a high potential for industrial applications and is an oleaginous microorgamism. Yarrowia lipolyptica can accumulate lipid content to approximately 40% of its dry cell weight and is a model organism for lipid accumulation and remobilization. See e.g., Nicaud, 2012, Yeast 29(10):409-18; Beopoulos et al., 2009, Biochimie 91(6):692-6; Bankar et al., 2009, Appl Microbiol Biotechnol. 84(5):847-65.

    Rhodotorula sp.

    [0248] Rhodotorula is unicellular, pigmented yeast. The oleaginous red yeast, Rhodotorula glutinis, has been shown to produce lipids and carotenoids from crude glycerol (Saenge et al., 2011, Process Biochemistry 46(1):210-8). Rhodotorula toruloides strains have been shown to be an efficient fed-batch fermentation system for improved biomass and lipid productivity (Li et al., 2007, Enzyme and Microbial Technology 41:312-7).

    Rhodosporidium toruloides

    [0249] Rhodosporidium toruloides is oleaginous yeast and useful for engineering lipid-production pathways (See e.g. Zhu et al., 2013, Nature Commun. 3:1112; Ageitos et al., 2011, Applied Microbiology and Biotechnology 90(4):1219-27).

    Candida boidinii

    [0250] Candida boidinii is methylotrophic yeast (it can grow on methanol). Like other methylotrophic species such as Hansenula polymorpha and Pichia pastoris, it provides an excellent platform for producing heterologous proteins. Yields in a multigram range of a secreted foreign protein have been reported. A computational method, IPRO, recently predicted mutations that experimentally switched the cofactor specificity of Candida boidinii xylose reductase from NADPH to NADH. See, e.g., Mattanovich et al., 2012, Methods Mol Biol. 824:329-58; Khoury et al., 2009, Protein Sci. 18(10):2125-38.

    Hansenula polymorpha (Pichia angusta)

    [0251] Hansenula polymorpha is methylotrophic yeast (see Candida boidinii). It can furthermore grow on a wide range of other substrates; it is thermo-tolerant and can assimilate nitrate (see also Kluyveromyces lactis). It has been applied to producing hepatitis B vaccines, insulin and interferon alpha-2a for the treatment of hepatitis C, furthermore to a range of technical enzymes. See, e.g., Xu et al., 2014, Virol Sin. 29(6):403-9.

    Kluyveromyces lactis

    [0252] Kluyveromyces lactis is yeast regularly applied to the production of kefir. It can grow on several sugars, most importantly on lactose which is present in milk and whey. It has successfully been applied among others for producing chymosin (an enzyme that is usually present in the stomach of calves) for producing cheese. Production takes place in fermenters on a 40,000 L scale. See, e.g., van Ooyen et al., 2006, FEMS Yeast Res. 6(3):381-92.

    Pichia pastoris

    [0253] Pichia pastoris is methylotrophic yeast (see Candida boidinii and Hansenula polymorpha). It provides an efficient platform for producing foreign proteins. Platform elements are available as a kit and it is worldwide used in academia for producing proteins. Strains have been engineered that can produce complex human N-glycan (yeast glycans are similar but not identical to those found in humans). See, e.g., Piirainen et al., 2014, N Biotechnol. 31(6):532-7.

    Physcomitrella spp.

    [0254] Physcomitrella mosses, when grown in suspension culture, have characteristics similar to yeast or other fungal cultures. This genera can be used for producing plant secondary metabolites, which can be difficult to produce in other types of cells.

    [0255] It will be appreciated that the recombinant host cell disclosed herein can comprise a plant cell, comprising a plant cell that is grown in a plant, a mammalian cell, an insect cell, a fungal cell, comprising a yeast cell, wherein the yeast cell is a cell from Saccharomyces cerevisiae, Schizosaccharomyces pombe, Yarrowia lipolytica, Candida glabrata, Ashbya gossypii, Cyberlindnera jadinii, Pichia pastoris, Kluyveromyces lactis, Hansenula polymorpha, Candida boidinii, Arxula adeninivorans, Xanthophyllomyces dendrorhous, or Candida albicans species or is a Saccharomycete or is a Saccharomyces cerevisiae cell, an algal cell or a bacterial cell, comprising Escherichia cells, Lactobacillus cells, Lactococcus cells, Cornebacterium cells, Acetobacter cells, Acinetobacter cells, or Pseudomonas cells.

    Steviol Glycoside Compositions

    [0256] Steviol glycosides do not necessarily have equivalent performance in different food systems. It is therefore desirable to have the ability to direct the synthesis to steviol glycoside compositions of choice. Recombinant hosts described herein can produce compositions that are selectively enriched for specific steviol glycosides (e.g., RebD or RebM) and have a consistent taste profile. As used herein, the term enriched is used to describe a steviol glycoside composition with an increased proportion of a particular steviol glycoside, compared to a steviol glycoside composition (extract) from a stevia plant. Thus, the recombinant hosts described herein can facilitate the production of compositions that are tailored to meet the sweetening profile desired for a given food product and that have a proportion of each steviol glycoside that is consistent from batch to batch. In some embodiments, hosts described herein do not produce or produce a reduced amount of undesired plant by-products found in Stevia extracts. Thus, steviol glycoside compositions produced by the recombinant hosts described herein are distinguishable from compositions derived from Stevia plants.

    [0257] The amount of an individual steviol glycoside (e.g., RebA, RebB, RebD, or RebM) accumulated can be from about 1 to about 7,000 mg/L, e.g., about 1 to about 10 mg/L, about 3 to about 10 mg/L, about 5 to about 20 mg/L, about 10 to about 50 mg/L, about 10 to about 100 mg/L, about 25 to about 500 mg/L, about 100 to about 1,500 mg/L, or about 200 to about 1,000 mg/L, at least about 1,000 mg/L, at least about 1,200 mg/L, at least about at least 1,400 mg/L, at least about 1,600 mg/L, at least about 1,800 mg/L, at least about 2,800 mg/L, or at least about 7,000 mg/L. In some aspects, the amount of an individual steviol glycoside can exceed 7,000 mg/L. The amount of a combination of steviol glycosides (e.g., RebA, RebB, RebD, or RebM) accumulated can be from about 1 mg/L to about 7,000 mg/L, e.g., about 200 to about 1,500, at least about 2,000 mg/L, at least about 3,000 mg/L, at least about 4,000 mg/L, at least about 5,000 mg/L, at least about 6,000 mg/L, or at least about 7,000 mg/L. In some aspects, the amount of a combination of steviol glycosides can exceed 7,000 mg/L. In general, longer culture times will lead to greater amounts of product. Thus, the recombinant microorganism can be cultured for from 1 day to 7 days, from 1 day to 5 days, from 3 days to 5 days, about 3 days, about 4 days, or about 5 days.

    [0258] It will be appreciated that the various genes and modules discussed herein can be present in two or more recombinant microorganisms rather than a single microorganism. When a plurality of recombinant microorganisms is used, they can be grown in a mixed culture to produce steviol and/or steviol glycosides. For example, a first microorganism can comprise one or more biosynthesis genes for producing a steviol glycoside precursor, while a second microorganism comprises steviol glycoside biosynthesis genes. The product produced by the second, or final microorganism is then recovered. It will also be appreciated that in some embodiments, a recombinant microorganism is grown using nutrient sources other than a culture medium and utilizing a system other than a fermenter.

    [0259] Alternatively, the two or more microorganisms each can be grown in a separate culture medium and the product of the first culture medium, e.g., steviol, can be introduced into second culture medium to be converted into a subsequent intermediate, or into an end product such as RebA. The product produced by the second, or final microorganism is then recovered. It will also be appreciated that in some embodiments, a recombinant microorganism is grown using nutrient sources other than a culture medium and utilizing a system other than a fermenter.

    [0260] Steviol glycosides and compositions obtained by the methods disclosed herein can be used to make food products, dietary supplements and sweetener compositions. See, e.g., WO 2011/153378, WO 2013/022989, WO 2014/122227, and WO 2014/122328.

    [0261] For example, substantially pure steviol or steviol glycoside such as RebM or RebD can be included in food products such as ice cream, carbonated beverages, fruit juices, yogurts, baked goods, chewing gums, hard and soft candies, and sauces. Substantially pure steviol or steviol glycoside can also be included in non-food products such as pharmaceutical products, medicinal products, dietary supplements and nutritional supplements. Substantially pure steviol or steviol glycosides may also be included in animal feed products for both the agriculture industry and the companion animal industry. Alternatively, a mixture of steviol and/or steviol glycosides can be made by culturing recombinant microorganisms separately, each producing a specific steviol or steviol glycoside, recovering the steviol or steviol glycoside in substantially pure form from each microorganism and then combining the compounds to obtain a mixture comprising each compound in the desired proportion. The recombinant microorganisms described herein permit more precise and consistent mixtures to be obtained compared to current Stevia products.

    [0262] In another alternative, a substantially pure steviol or steviol glycoside can be incorporated into a food product along with other sweeteners, e.g. saccharin, dextrose, sucrose, fructose, erythritol, aspartame, sucralose, monatin, or acesulfame potassium. The weight ratio of steviol or steviol glycoside relative to other sweeteners can be varied as desired to achieve a satisfactory taste in the final food product. See, eg., U.S. 2007/0128311. In some embodiments, the steviol or steviol glycoside may be provided with a flavor (e.g., citrus) as a flavor modulator.

    [0263] Compositions produced by a recombinant microorganism described herein can be incorporated into food products. For example, a steviol glycoside composition produced by a recombinant microorganism can be incorporated into a food product in an amount ranging from about 20 mg steviol glycoside/kg food product to about 1800 mg steviol glycoside/kg food product on a dry weight basis, depending on the type of steviol glycoside and food product. For example, a steviol glycoside composition produced by a recombinant microorganism can be incorporated into a dessert, cold confectionary (e.g., ice cream), dairy product (e.g., yogurt), or beverage (e.g., a carbonated beverage) such that the food product has a maximum of 500 mg steviol glycoside/kg food on a dry weight basis. A steviol glycoside composition produced by a recombinant microorganism can be incorporated into a baked good (e.g., a biscuit) such that the food product has a maximum of 300 mg steviol glycoside/kg food on a dry weight basis. A steviol glycoside composition produced by a recombinant microorganism can be incorporated into a sauce (e.g., chocolate syrup) or vegetable product (e.g., pickles) such that the food product has a maximum of 1000 mg steviol glycoside/kg food on a dry weight basis. A steviol glycoside composition produced by a recombinant microorganism can be incorporated into bread such that the food product has a maximum of 160 mg steviol glycoside/kg food on a dry weight basis. A steviol glycoside composition produced by a recombinant microorganism, plant, or plant cell can be incorporated into a hard or soft candy such that the food product has a maximum of 1600 mg steviol glycoside/kg food on a dry weight basis. A steviol glycoside composition produced by a recombinant microorganism, plant, or plant cell can be incorporated into a processed fruit product (e.g., fruit juices, fruit filling, jams, and jellies) such that the food product has a maximum of 1000 mg steviol glycoside/kg food on a dry weight basis. In some embodiments, a steviol glycoside composition produced herein is a component of a pharmaceutical composition. See, e.g., Steviol Glycosides Chemical and Technical Assessment 69th JECFA, 2007, prepared by Harriet Wallin, Food Agric. Org.; EFSA Panel on Food Additives and Nutrient Sources added to Food (ANS), Scientific Opinion on the safety of steviol glycosides for the proposed uses as a food additive, 2010, EFSA Journal 8(4):1537; U.S. Food and Drug Administration GRAS Notice 323; U.S Food and Drug Administration GRAS Notice Notice 329; WO 2011/037959; WO 2010/146463; WO 2011/046423; and WO 2011/056834.

    [0264] For example, such a steviol glycoside composition can have from 90-99 weight % RebA and an undetectable amount of stevia plant-derived contaminants, and be incorporated into a food product at from 25-1600 mg/kg, e.g., 100-500 mg/kg, 25-100 mg/kg, 250-1000 mg/kg, 50-500 mg/kg or 500-1000 mg/kg on a dry weight basis.

    [0265] Such a steviol glycoside composition can be a RebB-enriched composition having greater than 3 weight % RebB and be incorporated into the food product such that the amount of RebB in the product is from 25-1600 mg/kg, e.g., 100-500 mg/kg, 25-100 mg/kg, 250-1000 mg/kg, 50-500 mg/kg or 500-1000 mg/kg on a dry weight basis. Typically, the RebB-enriched composition has an undetectable amount of stevia plant-derived contaminants.

    [0266] Such a steviol glycoside composition can be a RebD-enriched composition having greater than 3 weight % RebD and be incorporated into the food product such that the amount of RebD in the product is from 25-1600 mg/kg, e.g., 100-500 mg/kg, 25-100 mg/kg, 250-1000 mg/kg, 50-500 mg/kg or 500-1000 mg/kg on a dry weight basis. Typically, the RebD-enriched composition has an undetectable amount of stevia plant-derived contaminants.

    [0267] Such a steviol glycoside composition can be a RebE-enriched composition having greater than 3 weight % RebE and be incorporated into the food product such that the amount of RebE in the product is from 25-1600 mg/kg, e.g., 100-500 mg/kg, 25-100 mg/kg, 250-1000 mg/kg, 50-500 mg/kg or 500-1000 mg/kg on a dry weight basis. Typically, the RebE-enriched composition has an undetectable amount of stevia plant-derived contaminants.

    [0268] Such a steviol glycoside composition can be a RebM-enriched composition having greater than 3 weight % RebM and be incorporated into the food product such that the amount of RebM in the product is from 25-1600 mg/kg, e.g., 100-500 mg/kg, 25-100 mg/kg, 250-1000 mg/kg, 50-500 mg/kg or 500-1000 mg/kg on a dry weight basis. Typically, the RebM-enriched composition has an undetectable amount of stevia plant-derived contaminants.

    [0269] In some embodiments, a substantially pure steviol or steviol glycoside is incorporated into a tabletop sweetener or cup-for-cup product. Such products typically are diluted to the appropriate sweetness level with one or more bulking agents, e.g., maltodextrins, known to those skilled in the art. Steviol glycoside compositions enriched for RebA, RebB, RebD, RebE, or RebM, can be package in a sachet, for example, at from 10,000 to 30,000 mg steviol glycoside/kg product on a dry weight basis, for tabletop use. In some embodiments, a steviol glycoside produced in vitro, in vivo, or by whole cell bioconversion

    [0270] The invention will be further described in the following examples, which do not limit the scope of the invention described in the claims.

    EXAMPLES

    [0271] The Examples that follow are illustrative of specific embodiments of the invention, and various uses thereof. They are set forth for explanatory purposes only, and are not to be taken as limiting the invention.

    Example 1

    LC-MS Analytical Procedures

    [0272] LC-MS analyses were performed on Waters ACQUITY UPLC (Waters Corporation) with a Waters ACQUITY UPLC BEH C18 column (2.150 mm, 1.7 m particles, 130 pore size) coupled to a Waters ACQUITY TQD triple quadropole mass spectrometer with electrospray ionization (ESI) in negative mode.

    [0273] Compound separation for Method A was achieved by a gradient of the two mobile phases: A (water with 0.1% formic acid) and B (MeCN with 0.1% formic acid) by increasing from 20% to 50 % B between 0.3 to 2.0 min, increasing to 100% B at 2.01 min, holding 100% B for 0.6 min, and re-equilibrating for 0.6 min.

    [0274] Compound separation for Method B was achieved by a gradient of the two mobile phases A (water with 0.1% formic acid) and B (MeCN with 0.1% formic acid) by increasing from 60% to 100% B in 2.5 min, holding 100% B for 0.1 min and re-equilibrating for 0.3 min.

    [0275] The flow rate was 0.6 mL/min, and the column temperature was 55 C. Steviol glycosides were monitored using SIM (Single Ion Monitoring) and quantified by comparing with authentic standards. See Table 1 for m/z trace and retention time values of steviol glycosides detected.

    TABLE-US-00001 TABLE 1 LC-MS Analytical Data for steviol and steviol glycosides. Compound MS Trace RT (min) Method FIG. Table steviol + 6Glc (isomer 1) 1289.53 0.87 A 3 (also referred to as compound 6.1) steviol + 7Glc (isomer 2) 1451.581 0.94 A 3 (also referred to as compound 7.2) RebD 1127.48 1.08 A RebM 1289.53 1.15 A steviol + 4Glc (#26) 965.42 1.21 A 4 (also referred to as compound 4.26) steviol + 5Glc (#24) 1127.48 1.18 A 7 (also referred to as compound 5.24) RebA 965.42 1.43 A 1,2-stevioside 803.37 1.43 A 6 rubusoside 641.32 1.67 A 5, 8 RebB 803.37 1.76 A steviol-1,2-bioside 641.32 1.80 A 5 19-SMG 525.27 1.98 A 4 13-SMG 479.26 2.04 A 4 ent-kaurenoic acid + 3Glc (isomer 1) 787.37 2.16 A 4 (also referred to as compound KA3.1) ent-kaurenoic acid + 3Glc (isomer 2) 787.37 2.28 A 5 (also referred to as compound KA3.2) ent-kaurenol + 3Glc (isomer 1) 773.4 2.36 A 5 co-eluted with ent-kaurenol + 3Glc (#6) (also referred to as compounds KL3.1 and KL3.6) ent-kaurenoic acid + 2Glc (#7) 625.32 2.35 A (also referred to as compound KA2.7) steviol 317.21 2.39 A ent-kaurenoic acid + 1Glc (#58) 439.27 0.69 B 3, 8 [also referred to as compound and KA1.58] 509.61

    Example 2

    Crude Lysate Preparation

    [0276] Colonies of E. coli strains constructed to express a UGT polypeptide were placed into sterile 96 deep well plates with 1 mL of NZCYM bacterial culture broth comprising ampicillin. The plate was sealed and samples were allowed to grow overnight at 37 C., shaking at 200 rpm. The following day (i.e., Day 2), 50 L of each culture was transferred to a new sterile 96 deep well plate with 1 mL of NZCYM bacterial culture broth comprising ampicillin and polypeptide expression inducers. The plate was sealed and samples were incubated at 20 C., shaking at 200 rpm for 20 h. On Day 3, the plate was centrifuged at 4000 rpm for 10 min at 4 C. After decanting the supernatant, 50 L of a buffer comprising Tris-HCl, MgCl.sub.2, CaCl.sub.2, and protease inhibitors was added to each well and cells were resuspended by shaking at 200 rpm for 5 min at 4 C. The contents of each well (i.e., cell slurries) were then transferred to a PCR plate and sealed before freezing at 80 C. overnight. Frozen cell slurries were thawed at room temperature for up to 30 min. If the thawing mix was not viscous due to cell lysing, samples were frozen and thawed again. When samples were nearly thawed, 25 L of binding buffer comprising DNase and MgCl.sub.2 was added to each well. The PCR plate was incubated at room temperature for 5 min, shaking at 500 rpm, until samples became less viscous. Finally, samples were centrifuged at 4000 rpm for 5 min, after which the supernatants were used to measure UGT activity, as described in Example 3.

    Example 3

    UGT Activity Assay

    [0277] UGT polypeptide samples prepared according to Example 2 were screened in vitro for activity on substrates including RebA, RebB, rubusoside, steviol, ent-kaurenoic acid, and 13-SMG by preparing a reaction mixture according to Table 2.

    TABLE-US-00002 TABLE 2 UGT Activity Assay Reaction Mixture. Component Volume (L) H.sub.2O 4.2 Alkaline Phosphatase 0.3 4 Buffer (10 mM Tris-HCl, 5 mM 7.5 MgCl.sub.2, 1 mM CaCl.sub.2) UDP-Glucose (1 mM) 9 Substrate 3 UGT Sample 6

    [0278] The reaction mixture was incubated overnight at 30 C. The reaction was stopped by adding 30 L of 100% DMSO. The resultant mixture was diluted further with 90 L 50% DMSO for LC-MS analysis according to Example 1. Both the products formed and the area-under-the-curve (AUC) values of each product are shown in Tables 3-7, organized by substrate.

    TABLE-US-00003 TABLE 3 UGT Activity on ent-kaurenoic acid. Activity ent-kaurenoic acid + 1Glc UGT Polypeptide SEQ ID NO: (#58) Production (AUC) UGT73C1 127 1095 UGT73C3 133 227 UGT73C5 135 2489 UGT73C6 137 699 UGT73E1 141 109 UGT74D1 143 119 UGT74G1 4 38967 UGT75B1 145 1409 UGT75L6 147 1208 UGT76E12 153 161 OleI 177 1086 UGT5 181 5547 SA Gtase 183 11088 UDPG1 185 460 UGT74F1 203 323 UGT75D1 205 2465 UGT84B2 207 31123 CaUGT2 209 446 UGT74F2-like UGT 211 20552

    TABLE-US-00004 TABLE 4 UGT Activity on steviol. Activity SEQ ID 13-SMG 19-SMG UGT Polypeptide NO: Production (AUC) Production (AUC) UGT73C1 127 9880 1235 UGT73C3 133 1850 295 UGT73C5 135 7100 2160 UGT73C6 137 2255 4980 UGT73C7 139 1570 N/A UGT73E1 141 2220 165 UGT74G1 4 N/A 172485 UGT75B1 145 N/A 230 UGT75L6 147 N/A 4615 UGT76E12 153 650 N/A UGT85C2 7 205575 N/A OleI 177 N/A 540 UGT5 181 N/A 1375 SA Gtase 183 N/A 10580 UDPG1 185 N/A 4420

    TABLE-US-00005 TABLE 5 UGT Activity on 13-SMG. Activity SEQ ID rubusoside steviol-1,2-bioside UGT Polypeptide NO: Production (AUC) Production (AUC) UGT73C1 127 550 N/A UGT73C6 137 1270 N/A UGT74G1 4 138650 N/A UGT85C2 7 865 N/A UGT91D2e-b 13 N/A 1080 EUGT11 16 N/A 10805 SA Gtase 183 4120 N/A UDPG1 185 2355 N/A UN32491 199 N/A 1065 UN1671 201 1185 N/A UGT74F1 203 950 N/A UGT75D1 205 99885 N/A UGT84B2 207 1390 N/A UGT74F2-like UGT 211 31415 N/A

    TABLE-US-00006 TABLE 6 UGT Activity on rubusoside. Activity 1,2-stevioside SEQ ID Production UGT Polypeptide NO: (AUC) UGT73C6 137 385 UGT91D2e-b 13 4680 CaUGT3 169 610 EUGT11 16 1900

    TABLE-US-00007 TABLE 7 UGT Activity on RebA. Activity SEQ ID steviol + 5Glc (#24) UGT Polypeptide NO: Production (AUC) EUGT11 16 4950 UN1671 201 52985

    [0279] As shown in Tables 3-7, 19-O-glycosylation, 13-O-glycosylation, and glycosyl-group glycosylation activity by UGT polypeptides on several substrates was observed, resulting in the formation of glycosides of ent-kaurenoic acid and steviol.

    TABLE-US-00008 TABLE 8 UGT Activity on 13-SMG and ent-kaurenoic acid. SEQ ID AUC rubusoside/ UGT Polypeptide NO: AUC KA1.58 UGT73C1 127 0.5 UGT73C6 137 1.8 UGT74G1 4 3.6 SA Gtase 183 0.4 UDPG1 185 5.1 UGT74F1 203 2.9 UGT75D1 205 40.5 UGT74F2-like UGT 211 1.5

    [0280] As shown in Table 8, UDPG1 (SEQ ID NO:185) and UGT75D1 (SEQ ID NO:205) produce relatively more rubusoside from 13-SMG than ent-kaurenoic acid+1Glc (#58) from ent-kaurenoic acid in vitro, compared to UGT74G1 (SEQ ID NO:4)

    Example 4

    Strain Engineering and Fermentation

    [0281] SA Gtase (SEQ ID NO:182, SEQ ID NO:183) was expressed with a p416-GPD vector in a steviol glycoside-producing S. cerevisiae strain comprising one or more copies of a recombinant gene encoding a GGPPS polypeptide (SEQ ID NO:19, SEQ ID NO:20), a recombinant gene encoding a truncated CDPS polypeptide (SEQ ID NO:39, SEQ ID NO:40), a recombinant gene encoding an KS polypeptide (SEQ ID NO:51, SEQ ID NO:52), a recombinant gene encoding a KO polypeptide (SEQ ID NO:59, SEQ ID NO:60), a recombinant gene encoding an ATR2 polypeptide (SEQ ID NO:91, SEQ ID NO:92), a recombinant gene encoding an EUGT11 polypeptide (SEQ ID NO:14/SEQ ID NO:15, SEQ ID NO:16), a recombinant gene encoding an KAH polypeptide (SEQ ID NO:93, SEQ ID NO:94), a recombinant gene encoding a CPR8 polypeptide (SEQ ID NO:85, SEQ ID NO:86), a recombinant gene encoding an UGT85C2 polypeptide (SEQ ID NO:5/SEQ ID NO:6/SEQ ID NO:149, SEQ ID NO:7) or a UGT85C2 variant (or functional homolog) of SEQ ID NO:7, a recombinant gene encoding a UGT74G1 polypeptide (SEQ ID NO:3, SEQ ID NO:4) of a UGT74G1 variant (or functional homolog) of SEQ ID NO:4, a recombinant gene encoding a UGT76G1 polypeptide (SEQ ID NO:8, SEQ ID NO:9) or a UGT76G1 variant (or functional homolog) of SEQ ID NO:9, and a recombinant gene encoding a UGT91D2e polypeptide (SEQ ID NO:10, SEQ ID NO:11) and a UGT91D2e variant (or functional homolog) of SEQ ID NO:11 such as a UGT91D2e-b (SEQ ID NO:12, SEQ ID NO:13).

    [0282] The strain was incubated in 1 mL synthetic complete (SC) uracil dropout media at 30 C. for five days, shaking at 400 rpm. 50 L of each culture was transferred into 50 L DMSO, incubated at 80 C. for 10 min, and centrifuged at 3220 g for 5 min. 15 L of the resulting supernatant was then transferred to 105 L 50% DMSO for LC-MS analysis, which was carried out according to Example 1. Normalized area-under-the-curve (AUC) values for LC-MS derived peaks corresponding to RebD and RebM were about 0.25 M/OD.sub.600 and 1.15 M/OD.sub.600, respectively. Ent-kaurenoic acid+2Glc (#7), ent-kaurenoic acid+3Glc (isomer 1), and ent-kaurenoic acid+3Glc (isomer 2) accumulated at levels of about 200 AUC/OD.sub.600, 15 AUC/OD.sub.600, and 1000 AUC/OD.sub.600, respectively. 13-SMG, RebA, and Reb B accumulated at levels of about 4.8 M/OD.sub.600, 2.5 M/OD.sub.600, and 0.25 M/OD.sub.600, respectively. Steviol+4Glc (#26), steviol+6Glc (isomer 1), steviol+7Glc (isomer 2), and kaurenol+3Glc (isomer 1 and/or 2) accumulated at levels of about 200 AUC/OD.sub.600, 15 AUC/OD.sub.600, 75 AUC/OD.sub.600, and 750 AUC/OD.sub.600, respectively.

    [0283] Having described the invention in detail and by reference to specific embodiments thereof, it will be apparent that modifications and variations are possible without departing from the scope of the invention defined in the appended claims. More specifically, although some aspects of the present invention are identified herein as particularly advantageous, it is contemplated that the present invention is not necessarily limited to these particular aspects of the invention.

    TABLE-US-00009 TABLE9 Sequencesdisclosedherein. SEQIDNO:3 ArtificialSequence atggcagagcaacaaaagatcaaaaagtcacctcacgtcttacttattccatttcctctg 60 caaggacatatcaacccattcatacaatttgggaaaagattgattagtaagggtgtaaag 120 acaacactggtaaccactatccacactttgaattctactctgaaccactcaaatactact 180 actacaagtatagaaattcaagctatatcagacggatgcgatgagggtggctttatgtct 240 gccggtgaatcttacttggaaacattcaagcaagtgggatccaagtctctggccgatcta 300 atcaaaaagttacagagtgaaggcaccacaattgacgccataatctacgattctatgaca 360 gagtgggttttagacgttgctatcgaatttggtattgatggaggttcctttttcacacaa 420 gcatgtgttgtgaattctctatactaccatgtgcataaagggttaatctctttaccattg 480 ggtgaaactgtttcagttccaggttttccagtgttacaacgttgggaaaccccattgatc 540 ttacaaaatcatgaacaaatacaatcaccttggtcccagatgttgtttggtcaattcgct 600 aacatcgatcaagcaagatgggtctttactaattcattctataagttagaggaagaggta 660 attgaatggactaggaagatctggaatttgaaagtcattggtccaacattgccatcaatg 720 tatttggacaaaagacttgatgatgataaagataatggtttcaatttgtacaaggctaat 780 catcacgaatgtatgaattggctggatgacaaaccaaaggaatcagttgtatatgttgct 840 ttcggctctcttgttaaacatggtccagaacaagttgaggagattacaagagcacttata 900 gactctgacgtaaactttttgtgggtcattaagcacaaagaggaggggaaactgccagaa 960 aacctttctgaagtgataaagaccggaaaaggtctaatcgttgcttggtgtaaacaattg 1020 gatgttttagctcatgaatctgtaggctgttttgtaacacattgcggattcaactctaca 1080 ctagaagccatttccttaggcgtacctgtcgttgcaatgcctcagttctccgatcagaca 1140 accaacgctaaacttttggacgaaatactaggggtgggtgtcagagttaaagcagacgag 1200 aatggtatcgtcagaagagggaacctagcttcatgtatcaaaatgatcatggaagaggaa 1260 agaggagttatcataaggaaaaacgcagttaagtggaaggatcttgcaaaggttgccgtc 1320 catgaaggcggctcttcagataatgatattgttgaatttgtgtccgaactaatcaaagcc 1380 taa 1383 SEQIDNO:4 S.rebaudiana MAEQQKIKKSPHVLLIPFPLQGHINPFIQFGKRLISKGVKTTLVTTIHTLNSTLNHSNTT 60 TTSIEIQAISDGCDEGGFMSAGESYLETFKQVGSKSLADLIKKLQSEGTTIDAIIYDSMT 120 EWVLDVAIEFGIDGGSFFTQACVVNSLYYHVHKGLISLPLGETVSVPGFPVLQRWETPLI 180 LQNHEQIQSPWSQMLFGQFANIDQARWVFTNSFYKLEEEVIEWTRKIWNLKVIGPTLPSM 240 YLDKRLDDDKDNGFNLYKANHHECMNWLDDKPKESVVYVAFGSLVKHGPEQVEEITRALI 300 DSDVNFLWVIKHKEEGKLPENLSEVIKTGKGLIVAWCKQLDVLAHESVGCFVTHCGFNST 360 LEAISLGVPVVAMPQFSDQTTNAKLLDEILGVGVRVKADENGIVRRGNLASCIKMIMEEE 420 RGVIIRKNAVKWKDLAKVAVHEGGSSDNDIVEFVSELIKA 460 SEQIDNO:5 S.rebaudiana atggatgcaatggctacaactgagaagaaaccacacgtcatcttcataccatttccagca 60 caaagccacattaaagccatgctcaaactagcacaacttctccaccacaaaggactccag 120 ataaccttcgtcaacaccgacttcatccacaaccagtttcttgaatcatcgggcccacat 180 tgtctagacggtgcaccgggtttccggttcgaaaccattccggatggtgtttctcacagt 240 ccggaagcgagcatcccaatcagagaatcactcttgagatccattgaaaccaacttcttg 300 gatcgtttcattgatcttgtaaccaaacttccggatcctccgacttgtattatctcagat 360 gggttcttgtcggttttcacaattgacgctgcaaaaaagcttggaattccggtcatgatg 420 tattggacacttgctgcctgtgggttcatgggtttttaccatattcattctctcattgag 480 aaaggatttgcaccacttaaagatgcaagttacttgacaaatgggtatttggacaccgtc 540 attgattgggttccgggaatggaaggcatccgtctcaaggatttcccgctggactggagc 600 actgacctcaatgacaaagttttgatgttcactacggaagctcctcaaaggtcacacaag 660 gtttcacatcatattttccacacgttcgatgagttggagcctagtattataaaaactttg 720 tcattgaggtataatcacatttacaccatcggcccactgcaattacttcttgatcaaata 780 cccgaagagaaaaagcaaactggaattacgagtctccatggatacagtttagtaaaagaa 840 gaaccagagtgtttccagtggcttcagtctaaagaaccaaattccgtcgtttatgtaaat 900 tttggaagtactacagtaatgtctttagaagacatgacggaatttggttggggacttgct 960 aatagcaaccattatttcctttggatcatccgatcaaacttggtgataggggaaaatgca 1020 gttttgccccctgaacttgaggaacatataaagaaaagaggctttattgctagctggtgt 1080 tcacaagaaaaggtcttgaagcacccttcggttggagggttcttgactcattgtgggtgg 1140 ggatcgaccatcgagagcttgtctgctggggtgccaatgatatgctggccttattcgtgg 1200 gaccagctgaccaactgtaggtatatatgcaaagaatgggaggttgggctcgagatggga 1260 accaaagtgaaacgagatgaagtcaagaggcttgtacaagagttgatgggagaaggaggt 1320 cacaaaatgaggaacaaggctaaagattggaaagaaaaggctcgcattgcaatagctcct 1380 aacggttcatcttctttgaacatagacaaaatggtcaaggaaatcaccgtgctagcaaga 1440 aactag 1446 SEQIDNO:6 ArtificialSequence atggatgcaatggcaactactgagaaaaagcctcatgtgatcttcattccatttcctgca 60 caatctcacataaaggcaatgctaaagttagcacaactattacaccataagggattacag 120 ataactttcgtgaataccgacttcatccataatcaatttctggaatctagtggccctcat 180 tgtttggacggagccccagggtttagattcgaaacaattcctgacggtgtttcacattcc 240 ccagaggcctccatcccaataagagagagtttactgaggtcaatagaaaccaactttttg 300 gatcgtttcattgacttggtcacaaaacttccagacccaccaacttgcataatctctgat 360 ggctttctgtcagtgtttactatcgacgctgccaaaaagttgggtatcccagttatgatg 420 tactggactcttgctgcatgcggtttcatgggtttctatcacatccattctcttatcgaa 480 aagggttttgctccactgaaagatgcatcatacttaaccaacggctacctggatactgtt 540 attgactgggtaccaggtatggaaggtataagacttaaagattttcctttggattggtct 600 acagaccttaatgataaagtattgatgtttactacagaagctccacaaagatctcataag 660 gtttcacatcatatctttcacacctttgatgaattggaaccatcaatcatcaaaaccttg 720 tctctaagatacaatcatatctacactattggtccattacaattacttctagatcaaatt 780 cctgaagagaaaaagcaaactggtattacatccttacacggctactctttagtgaaagag 840 gaaccagaatgttttcaatggctacaaagtaaagagcctaattctgtggtctacgtcaac 900 ttcggaagtacaacagtcatgtccttggaagatatgactgaatttggttggggccttgct 960 aattcaaatcattactttctatggattatcaggtccaatttggtaataggggaaaacgcc 1020 gtattacctccagaattggaggaacacatcaaaaagagaggtttcattgcttcctggtgt 1080 tctcaggaaaaggtattgaaacatccttctgttggtggtttccttactcattgcggttgg 1140 ggctctacaatcgaatcactaagtgcaggagttccaatgatttgttggccatattcatgg 1200 gaccaacttacaaattgtaggtatatctgtaaagagtgggaagttggattagaaatggga 1260 acaaaggttaaacgtgatgaagtgaaaagattggttcaggagttgatgggggaaggtggc 1320 cacaagatgagaaacaaggccaaagattggaaggaaaaagccagaattgctattgctcct 1380 aacgggtcatcctctctaaacattgataagatggtcaaagagattacagtcttagccaga 1440 aactaa 1446 SEQIDNO:7 S.rebaudiana MDAMATTEKKPHVIFIPFPAQSHIKAMLKLAQLLHHKGLQITFVNTDFIHNQFLESSGPH 60 CLDGAPGFRFETIPDGVSHSPEASIPIRESLLRSIETNFLDRFIDLVTKLPDPPTCIISD 120 GFLSVFTIDAAKKLGIPVMMYWTLAACGFMGFYHIHSLIEKGFAPLKDASYLTNGYLDTV 180 IDWVPGMEGIRLKDFPLDWSTDLNDKVLMFTTEAPQRSHKVSHHIFHTFDELEPSIIKTL 240 SLRYNHIYTIGPLQLLLDQIPEEKKQTGITSLHGYSLVKEEPECFQWLQSKEPNSVVYVN 300 FGSTTVMSLEDMTEFGWGLANSNHYFLWIIRSNLVIGENAVLPPELEEHIKKRGFIASWC 360 SQEKVLKHPSVGGFLTHCGWGSTIESLSAGVPMICWPYSWDQLTNCRYICKEWEVGLEMG 420 TKVKRDEVKRLVQELMGEGGHKMRNKAKDWKEKARIAIAPNGSSSLNIDKMVKEITVLAR 480 N 481 SEQIDNO:8 ArtificialSequence atggaaaacaagaccgaaacaacagttagacgtaggcgtagaatcattctgtttccagta 60 ccttttcaagggcacatcaatccaatactacaactagccaacgttttgtactctaaaggt 120 ttttctattacaatctttcacaccaatttcaacaaaccaaaaacatccaattacccacat 180 ttcacattcagattcatacttgataatgatccacaagatgaacgtatttcaaacttacct 240 acccacggtcctttagctggaatgagaattccaatcatcaatgaacatggtgccgatgag 300 cttagaagagaattagagttacttatgttggcatccgaagaggacgaggaagtctcttgt 360 ctgattactgacgctctatggtactttgcccaatctgtggctgatagtttgaatttgagg 420 agattggtactaatgacatccagtctgtttaactttcacgctcatgttagtttaccacaa 480 tttgacgaattgggatacttggaccctgatgacaagactaggttagaggaacaggcctct 540 ggttttcctatgttgaaagtcaaagatatcaagtctgcctattctaattggcaaatcttg 600 aaagagatcttaggaaagatgatcaaacagacaaaggcttcatctggagtgatttggaac 660 agtttcaaagagttagaagagtctgaattggagactgtaatcagagaaattccagcacct 720 tcattcctgataccattaccaaaacatttgactgcttcctcttcctctttgttggatcat 780 gacagaacagtttttcaatggttggaccaacaaccacctagttctgttttgtacgtgtca 840 tttggtagtacttctgaagtcgatgaaaaggacttccttgaaatcgcaagaggcttagtc 900 gatagtaagcagtcattcctttgggtcgtgcgtccaggtttcgtgaaaggctcaacatgg 960 gtcgaaccacttccagatggttttctaggcgaaagaggtagaatagtcaaatgggttcct 1020 caacaggaagttttagctcatggcgctattggggcattctggactcattccggatggaat 1080 tcaactttagaatcagtatgcgaaggggtacctatgatcttttcagattttggtcttgat 1140 caaccactgaacgcaagatacatgtctgatgttttgaaagtgggtgtatatctagaaaat 1200 ggctgggaaaggggtgaaatagctaatgcaataagacgtgttatggttgatgaagagggg 1260 gagtatatcagacaaaacgcaagagtgctgaagcaaaaggccgacgtttctctaatgaag 1320 ggaggctcttcatacgaatccttagaatctcttgtttcctacatttcatcactgtaa 1377 SEQIDNO:9 S.rebaudiana MENKTETTVRRRRRIILFPVPFQGHINPILQLANVLYSKGFSITIFHTNFNKPKTSNYPH 60 FTFRFILDNDPQDERISNLPTHGPLAGMRIPIINEHGADELRRELELLMLASEEDEEVSC 120 LITDALWYFAQSVADSLNLRRLVLMTSSLFNFHAHVSLPQFDELGYLDPDDKTRLEEQAS 180 GFPMLKVKDIKSAYSNWQILKEILGKMIKQTKASSGVIWNSFKELEESELETVIREIPAP 240 SFLIPLPKHLTASSSSLLDHDRTVFQWLDQQPPSSVLYVSFGSTSEVDEKDFLEIARGLV 300 DSKQSFLWVVRPGFVKGSTWVEPLPDGFLGERGRIVKWVPQQEVLAHGAIGAFWTHSGWN 360 STLESVCEGVPMIFSDFGLDQPLNARYMSDVLKVGVYLENGWERGEIANAIRRVMVDEEG 420 EYIRQNARVLKQKADVSLMKGGSSYESLESLVSYISSL 458 SEQIDNO:10 ArtificialSequence atggctacatctgattctattgttgatgacaggaagcagttgcatgtggctactttccct 60 tggcttgctttcggtcatatactgccttacctacaactatcaaaactgatagctgaaaaa 120 ggacataaagtgtcattcctttcaacaactagaaacattcaaagattatcttcccacata 180 tcaccattgattaacgtcgttcaattgacacttccaagagtacaggaattaccagaagat 240 gctgaagctacaacagatgtgcatcctgaagatatcccttacttgaaaaaggcatccgat 300 ggattacagcctgaggtcactagattccttgagcaacacagtccagattggatcatatac 360 gactacactcactattggttgccttcaattgcagcatcactaggcatttctagggcacat 420 ttcagtgtaaccacaccttgggccattgcttacatgggtccatccgctgatgctatgatt 480 aacggcagtgatggtagaactaccgttgaagatttgacaaccccaccaaagtggtttcca 540 tttccaactaaagtctgttggagaaaacacgacttagcaagactggttccatacaaggca 600 ccaggaatctcagacggctatagaatgggtttagtccttaaagggtctgactgcctattg 660 tctaagtgttaccatgagtttgggacacaatggctaccacttttggaaacattacaccaa 720 gttcctgtcgtaccagttggtctattacctccagaaatccctggtgatgagaaggacgag 780 acttgggtttcaatcaaaaagtggttagacgggaagcaaaaaggctcagtggtatatgtg 840 gcactgggttccgaagttttagtatctcaaacagaagttgtggaacttgccttaggtttg 900 gaactatctggattgccatttgtctgggcctacagaaaaccaaaaggccctgcaaagtcc 960 gattcagttgaattgccagacggctttgtcgagagaactagagatagagggttggtatgg 1020 acttcatgggctccacaattgagaatcctgagtcacgaatctgtgtgcggtttcctaaca 1080 cattgtggttctggttctatagttgaaggactgatgtttggtcatccacttatcatgttg 1140 ccaatctttggtgaccagcctttgaatgcacgtctgttagaagataaacaagttggaatt 1200 gaaatcccacgtaatgaggaagatggatgtttaaccaaggagtctgtggccagatcatta 1260 cgttccgttgtcgttgaaaaggaaggcgaaatctacaaggccaatgcccgtgaactttca 1320 aagatctacaatgacacaaaagtagagaaggaatatgtttctcaatttgtagattaccta 1380 gagaaaaacgctagagccgtagctattgatcatgaatcctaa 1422 SEQIDNO:11 S.rebaudiana MATSDSIVDDRKQLHVATFPWLAFGHILPYLQLSKLIAEKGHKVSFLSTTRNIQRLSSHI 60 SPLINVVQLTLPRVQELPEDAEATTDVHPEDIPYLKKASDGLQPEVTRFLEQHSPDWIIY 120 DYTHYWLPSIAASLGISRAHFSVTTPWAIAYMGPSADAMINGSDGRTTVEDLTTPPKWFP 180 FPTKVCWRKHDLARLVPYKAPGISDGYRMGLVLKGSDCLLSKCYHEFGTQWLPLLETLHQ 240 VPVVPVGLLPPEIPGDEKDETWVSIKKWLDGKQKGSVVYVALGSEVLVSQTEVVELALGL 300 ELSGLPFVWAYRKPKGPAKSDSVELPDGFVERTRDRGLVWTSWAPQLRILSHESVCGFLT 360 HCGSGSIVEGLMFGHPLIMLPIFGDQPLNARLLEDKQVGIEIPRNEEDGCLTKESVARSL 420 RSVVVEKEGEIYKANARELSKIYNDTKVEKEYVSQFVDYLEKNARAVAIDHES 473 SEQIDNO:12 ArtificialSequence atggctacttctgattccatcgttgacgatagaaagcaattgcatgttgctacttttcca 60 tggttggctttcggtcatattttgccatacttgcaattgtccaagttgattgctgaaaag 120 ggtcacaaggtttcattcttgtctaccaccagaaacatccaaagattgtcctctcatatc 180 tccccattgatcaacgttgttcaattgactttgccaagagtccaagaattgccagaagat 240 gctgaagctactactgatgttcatccagaagatatcccttacttgaaaaaggcttccgat 300 ggtttacaaccagaagttactagattcttggaacaacattccccagattggatcatctac 360 gattatactcattactggttgccatccattgctgcttcattgggtatttctagagcccat 420 ttctctgttactactccatgggctattgcttatatgggtccatctgctgatgctatgatt 480 aacggttctgatggtagaactaccgttgaagatttgactactccaccaaagtggtttcca 540 tttccaacaaaagtctgttggagaaaacacgatttggctagattggttccatacaaagct 600 ccaggtatttctgatggttacagaatgggtatggttttgaaaggttccgattgcttgttg 660 tctaagtgctatcatgaattcggtactcaatggttgcctttgttggaaacattgcatcaa 720 gttccagttgttccagtaggtttgttgccaccagaaattccaggtgacgaaaaagacgaa 780 acttgggtttccatcaaaaagtggttggatggtaagcaaaagggttctgttgtttatgtt 840 gctttgggttccgaagctttggtttctcaaaccgaagttgttgaattggctttgggtttg 900 gaattgtctggtttgccatttgtttgggcttacagaaaacctaaaggtccagctaagtct 960 gattctgttgaattgccagatggtttcgttgaaagaactagagatagaggtttggtttgg 1020 acttcttgggctccacaattgagaattttgtctcatgaatccgtctgtggtttcttgact 1080 cattgtggttctggttctatcgttgaaggtttgatgtttggtcacccattgattatgttg 1140 ccaatctttggtgaccaaccattgaacgctagattattggaagataagcaagtcggtatc 1200 gaaatcccaagaaatgaagaagatggttgcttgaccaaagaatctgttgctagatctttg 1260 agatccgttgtcgttgaaaaagaaggtgaaatctacaaggctaacgctagagaattgtcc 1320 aagatctacaacgataccaaggtcgaaaaagaatacgtttcccaattcgttgactacttg 1380 gaaaagaatgctagagctgttgccattgatcatgaatcttga 1422 SEQIDNO:13 ArtificialSequence MATSDSIVDDRKQLHVATFPWLAFGHILPYLQLSKLIAEKGHKVSFLSTTRNIQRLSSHI 60 SPLINVVQLTLPRVQELPEDAEATTDVHPEDIPYLKKASDGLQPEVTRFLEQHSPDWIIY 120 DYTHYWLPSIAASLGISRAHFSVTTPWAIAYMGPSADAMINGSDGRTTVEDLTTPPKWFP 180 FPTKVCWRKHDLARLVPYKAPGISDGYRMGMVLKGSDCLLSKCYHEFGTQWLPLLETLHQ 240 VPVVPVGLLPPEIPGDEKDETWVSIKKWLDGKQKGSVVYVALGSEALVSQTEVVELALGL 300 ELSGLPFVWAYRKPKGPAKSDSVELPDGFVERTRDRGLVWTSWAPQLRILSHESVCGFLT 360 HCGSGSIVEGLMFGHPLIMLPIFGDQPLNARLLEDKQVGIEIPRNEEDGCLTKESVARSL 420 RSVVVEKEGEIYKANARELSKIYNDTKVEKEYVSQFVDYLEKNARAVAIDHES 473 SEQIDNO:14 Oryzasativa atggactccggctactcctcctcctacgccgccgccgccgggatgcacgtcgtgatctgc 60 ccgtggctcgccttcggccacctgctcccgtgcctcgacctcgcccagcgcctcgcgtcg 120 cggggccaccgcgtgtcgttcgtctccacgccgcggaacatatcccgcctcccgccggtg 180 cgccccgcgctcgcgccgctcgtcgccttcgtggcgctgccgctcccgcgcgtcgagggg 240 ctccccgacggcgccgagtccaccaacgacgtcccccacgacaggccggacatggtcgag 300 ctccaccggagggccttcgacgggctcgccgcgcccttctcggagttcttgggcaccgcg 360 tgcgccgactgggtcatcgtcgacgtcttccaccactgggccgcagccgccgctctcgag 420 cacaaggtgccatgtgcaatgatgttgttgggctctgcacatatgatcgcttccatagca 480 gacagacggctcgagcgcgcggagacagagtcgcctgcggctgccgggcagggacgccca 540 gcggcggcgccaacgttcgaggtggcgaggatgaagttgatacgaaccaaaggctcatcg 600 ggaatgtccctcgccgagcgcttctccttgacgctctcgaggagcagcctcgtcgtcggg 660 cggagctgcgtggagttcgagccggagaccgtcccgctcctgtcgacgctccgcggtaag 720 cctattaccttccttggccttatgccgccgttgcatgaaggccgccgcgaggacggcgag 780 gatgccaccgtccgctggctcgacgcgcagccggccaagtccgtcgtgtacgtcgcgcta 840 ggcagcgaggtgccactgggagtggagaaggtccacgagctcgcgctcgggctggagctc 900 gccgggacgcgcttcctctgggctcttaggaagcccactggcgtctccgacgccgacctc 960 ctccccgccggcttcgaggagcgcacgcgcggccgcggcgtcgtggcgacgagatgggtt 1020 cctcagatgagcatactggcgcacgccgccgtgggcgcgttcctgacccactgcggctgg 1080 aactcgaccatcgaggggctcatgttcggccacccgcttatcatgctgccgatcttcggc 1140 gaccagggaccgaacgcgcggctaatcgaggcgaagaacgccggattgcaggtggcaaga 1200 aacgacggcgatggatcgttcgaccgagaaggcgtcgcggcggcgattcgtgcagtcgcg 1260 gtggaggaagaaagcagcaaagtgtttcaagccaaagccaagaagctgcaggagatcgtc 1320 gcggacatggcctgccatgagaggtacatcgacggattcattcagcaattgagatcttac 1380 aaggattga 1389 SEQIDNO:15 ArtificialSequence atggatagtggctactcctcatcttatgctgctgccgctggtatgcacgttgtgatctgc 60 ccttggttggcctttggtcacctgttaccatgtctggatttagcccaaagactggcctca 120 agaggccatagagtatcatttgtgtctactcctagaaatatctctcgtttaccaccagtc 180 agacctgctctagctcctctagttgcattcgttgctcttccacttccaagagtagaagga 240 ttgccagacggcgctgaatctactaatgacgtaccacatgatagacctgacatggtcgaa 300 ttgcatagaagagcctttgatggattggcagctccattttctgagttcctgggcacagca 360 tgtgcagactgggttatagtcgatgtatttcatcactgggctgctgcagccgcattggaa 420 cataaggtgccttgtgctatgatgttgttagggtcagcacacatgatcgcatccatagct 480 gatagaagattggaaagagctgaaacagaatccccagccgcagcaggacaaggtaggcca 540 gctgccgccccaacctttgaagtggctagaatgaaattgattcgtactaaaggtagttca 600 gggatgagtcttgctgaaaggttttctctgacattatctagatcatcattagttgtaggt 660 agatcctgcgtcgagttcgaacctgaaacagtacctttactatctactttgagaggcaaa 720 cctattactttccttggtctaatgcctccattacatgaaggaaggagagaagatggtgaa 780 gatgctactgttaggtggttagatgcccaacctgctaagtctgttgtttacgttgcattg 840 ggttctgaggtaccactaggggtggaaaaggtgcatgaattagcattaggacttgagctg 900 gccggaacaagattcctttgggctttgagaaaaccaaccggtgtttctgacgccgacttg 960 ctaccagctgggttcgaagagagaacaagaggccgtggtgtcgttgctactagatgggtc 1020 ccacaaatgagtattctagctcatgcagctgtaggggcctttctaacccattgcggttgg 1080 aactcaacaatagaaggactgatgtttggtcatccacttattatgttaccaatctttggc 1140 gatcagggacctaacgcaagattgattgaggcaaagaacgcaggtctgcaggttgcacgt 1200 aatgatggtgatggttcctttgatagagaaggcgttgcagctgccatcagagcagtcgcc 1260 gttgaggaagagtcatctaaagttttccaagctaaggccaaaaaattacaagagattgtg 1320 gctgacatggcttgtcacgaaagatacatcgatggtttcatccaacaattgagaagttat 1380 aaagactaa 1389 SEQIDNO:16 Oryzasativa MDSGYSSSYAAAAGMHVVICPWLAFGHLLPCLDLAQRLASRGHRVSFVSTPRNISRLPPV 60 RPALAPLVAFVALPLPRVEGLPDGAESTNDVPHDRPDMVELHRRAFDGLAAPFSEFLGTA 120 CADWVIVDVFHHWAAAAALEHKVPCAMMLLGSAHMIASIADRRLERAETESPAAAGQGRP 180 AAAPTFEVARMKLIRTKGSSGMSLAERFSLTLSRSSLVVGRSCVEFEPETVPLLSTLRGK 240 PITFLGLMPPLHEGRREDGEDATVRWLDAQPAKSVVYVALGSEVPLGVEKVHELALGLEL 300 AGTRFLWALRKPTGVSDADLLPAGFEERTRGRGVVATRWVPQMSILAHAAVGAFLTHCGW 360 NSTIEGLMFGHPLIMLPIFGDQGPNARLIEAKNAGLQVARNDGDGSFDREGVAAAIRAVA 420 VEEESSKVFQAKAKKLQEIVADMACHERYIDGFIQQLRSYKD 462 SEQIDNO:17 ArtificialSequence MDSGYSSSYAAAAGMHVVICPWLAFGHLLPCLDLAQRLASRGHRVSFVSTPRNISRLPPV 60 RPALAPLVAFVALPLPRVEGLPDGAESTNDVPHDRPDMVELHRRAFDGLAAPFSEFLGTA 120 CADWVIVDVFHHWAAAAALEHKVPCAMMLLGSAHMIASIADRRLERAETESPAAAGQGRP 180 AAAPTFEVARMKLIRTKGSSGMSLAERFSLTLSRSSLVVGRSCVEFEPETVPLLSTLRGK 240 PITFLGLLPPEIPGDEKDETWVSIKKWLDGKQKGSVVYVALGSEALVSQTEVVELALGLE 300 LSGLPFVWAYRKPKGPAKSDSVELPDGFVERTRDRGLVWTSWAPQLRILSHESVCGFLTH 360 CGSGSIVEGLMFGHPLIMLPIFGDQPLNARLLEDKQVGIEIARNDGDGSFDREGVAAAIR 420 AVAVEEESSKVFQAKAKKLQEIVADMACHERYIDGFIQQLRSYKD 465 SEQIDNO:18 ArtificialSequence MATSDSIVDDRKQLHVATFPWLAFGHILPYLQLSKLIAEKGHKVSFLSTTRNIQRLSSHI 60 SPLINVVQLTLPRVQELPEDAEATTDVHPEDIPYLKKASDGLQPEVTRFLEQHSPDWIIY 120 DYTHYWLPSIAASLGISRAHFSVTTPWAIAYMGPSADAMINGSDGRTTVEDLTTPPKWFP 180 FPTKVCWRKHDLARLVPYKAPGISDGYRMGMVLKGSDCLLSKCYHEFGTQWLPLLETLHQ 240 VPVVPVGLMPPLHEGRREDGEDATVRWLDAQPAKSVVYVALGSEVPLGVEKVHELALGLE 300 LAGTRFLWALRKPTGVSDADLLPAGFEERTRGRGVVATRWVPQMSILAHAAVGAFLTHCG 360 WNSTIEGLMFGHPLIMLPIFGDQGPNARLIEAKNAGLQVPRNEEDGCLTKESVARSLRSV 420 VVEKEGEIYKANARELSKIYNDTKVEKEYVSQFVDYLEKNARAVAIDHES 470 SEQIDNO:19 Steviarebaudiana atggctttggtaaacccaaccgctcttttctatggtacctctatcagaacaagacctaca 60 aacttactaaatccaactcaaaagctaagaccagtttcatcatcttccttaccttctttc 120 tcatcagttagtgcgattcttactgaaaaacatcaatctaatccttctgagaacaacaat 180 ttgcaaactcatctagaaactcctttcaactttgatagttatatgttggaaaaagtcaac 240 atggttaacgaggcgcttgatgcatctgtcccactaaaagacccaatcaaaatccatgaa 300 tccatgagatactctttattggcaggcggtaagagaatcagaccaatgatgtgtattgca 360 gcctgcgaaatagtcggaggtaatatccttaacgccatgccagccgcatgtgccgtggaa 420 atgattcatactatgtctttggtgcatgacgatcttccatgtatggataatgatgacttc 480 agaagaggtaaacctatttcacacaaggtctacggggaggaaatggcagtattgaccggc 540 gatgctttactaagtttatctttcgaacatatagctactgctacaaagggtgtatcaaag 600 gatagaatcgtcagagctataggggagttggcccgttcagttggctccgaaggtttagtg 660 gctggacaagttgtagatatcttgtcagagggtgctgatgttggattagatcacctagaa 720 tacattcacatccacaaaacagcaatgttgcttgagtcctcagtagttattggcgctatc 780 atgggaggaggatctgatcagcagatcgaaaagttgagaaaattcgctagatctattggt 840 ctactattccaagttgtggatgacattttggatgttacaaaatctaccgaagagttgggg 900 aaaacagctggtaaggatttgttgacagataagacaacttacccaaagttgttaggtata 960 gaaaagtccagagaatttgccgaaaaacttaacaaggaagcacaagagcaattaagtggc 1020 tttgatagacgtaaggcagctcctttgatcgcgttagccaactacaatgcgtaccgtcaa 1080 aattga 1086 SEQIDNO:20 Steviarebaudiana MALVNPTALFYGTSIRTRPTNLLNPTQKLRPVSSSSLPSFSSVSAILTEKHQSNPSENNN 60 LQTHLETPFNFDSYMLEKVNMVNEALDASVPLKDPIKIHESMRYSLLAGGKRIRPMMCIA 120 ACEIVGGNILNAMPAACAVEMIHTMSLVHDDLPCMDNDDFRRGKPISHKVYGEEMAVLTG 180 DALLSLSFEHIATATKGVSKDRIVRAIGELARSVGSEGLVAGQVVDILSEGADVGLDHLE 240 YIHIHKTAMLLESSVVIGAIMGGGSDQQIEKLRKFARSIGLLFQVVDDILDVTKSTEELG 300 KTAGKDLLTDKTTYPKLLGIEKSREFAEKLNKEAQEQLSGFDRRKAAPLIALANYNAYRQ 360 N 361 SEQIDNO:21 ArtificialSequence atggctgagcaacaaatatctaacttgctgtctatgtttgatgcttcacatgctagtcag 60 aaattagaaattactgtccaaatgatggacacataccattacagagaaacgcctccagat 120 tcctcatcttctgaaggcggttcattgtctagatacgacgagagaagagtctctttgcct 180 ctcagtcataatgctgcctctccagatattgtatcacaactatgtttttccactgcaatg 240 tcttcagagttgaatcacagatggaaatctcaaagattaaaggtggccgattctccttac 300 aactatatcctaacattaccatcaaaaggaattagaggtgcctttatcgattccctgaac 360 gtatggttggaggttccagaggatgaaacatcagtcatcaaggaagttattggtatgctc 420 cacaactcttcattaatcattgatgacttccaagataattctccacttagaagaggaaag 480 ccatctacccatacagtcttcggccctgcccaggctatcaatactgctacttacgttata 540 gttaaagcaatcgaaaagatacaagacatagtgggacacgatgcattggcagatgttacg 600 ggtactattacaactattttccaaggtcaggccatggacttgtggtggacagcaaatgca 660 atcgttccatcaatacaggaatacttacttatggtaaacgataaaaccggtgctctcttt 720 agactgagtttggagttgttagctctgaattccgaagccagtatttctgactctgcttta 780 gaaagtttatctagtgctgtttccttgctaggtcaatacttccaaatcagagacgactat 840 atgaacttgatcgataacaagtatacagatcagaaaggcttctgcgaagatcttgatgaa 900 ggcaagtactcactaacacttattcatgccctccaaactgattcatccgatctactgacc 960 aacatcctttcaatgagaagagtgcaaggaaagttaacggcacaaaagagatgttggttc 1020 tggaaatga 1029 SEQIDNO:22 Gibberellafujikuroi MAEQQISNLLSMFDASHASQKLEITVQMMDTYHYRETPPDSSSSEGGSLSRYDERRVSLP 60 LSHNAASPDIVSQLCFSTAMSSELNHRWKSQRLKVADSPYNYILTLPSKGIRGAFIDSLN 120 VWLEVPEDETSVIKEVIGMLHNSSLIIDDFQDNSPLRRGKPSTHTVFGPAQAINTATYVI 180 VKAIEKIQDIVGHDALADVTGTITTIFQGQAMDLWWTANAIVPSIQEYLLMVNDKTGALF 240 RLSLELLALNSEASISDSALESLSSAVSLLGQYFQIRDDYMNLIDNKYTDQKGFCEDLDE 300 GKYSLTLIHALQTDSSDLLTNILSMRRVQGKLTAQKRCWFWK 342 SEQIDNO:23 ArtificialSequence atggaaaagactaaggagaaagcagaacgtatcttgctggagccatacagatacttatta 60 caactaccaggaaagcaagtccgttctaaactatcacaagcgttcaatcactggttaaaa 120 gttcctgaagataagttacaaatcattattgaagtcacagaaatgctacacaatgcttct 180 ttactgatcgatgatatagaggattcttccaaactgagaagaggttttcctgtcgctcat 240 tccatatacggggtaccaagtgtaatcaactcagctaattacgtctacttcttgggattg 300 gaaaaagtattgacattagatcatccagacgctgtaaagctattcaccagacaacttctt 360 gaattgcatcaaggtcaaggtttggatatctattggagagacacttatacttgcccaaca 420 gaagaggagtacaaagcaatggttctacaaaagactggcggtttgttcggacttgccgtt 480 ggtctgatgcaacttttctctgattacaaggaggacttaaagcctctgttggataccttg 540 ggcttgtttttccagattagagatgactacgctaacttacattcaaaggaatattcagaa 600 aacaaatcattctgtgaagatttgactgaagggaagtttagttttccaacaatccacgcc 660 atttggtcaagaccagaatctactcaagtgcaaaacattctgcgtcagagaacagagaat 720 attgacatcaaaaagtattgtgttcagtacttggaagatgttggttcttttgcttacaca 780 agacatacacttagagaattagaggcaaaagcatacaagcaaatagaagcctgtggaggc 840 aatccttctctagtggcattggttaaacatttgtccaaaatgttcaccgaggaaaacaag 900 taa 903 SEQIDNO:24 Musmusculus MEKTKEKAERILLEPYRYLLQLPGKQVRSKLSQAFNHWLKVPEDKLQIIIEVTEMLHNAS 60 LLIDDIEDSSKLRRGFPVAHSIYGVPSVINSANYVYFLGLEKVLTLDHPDAVKLFTRQLL 120 ELHQGQGLDIYWRDTYTCPTEEEYKAMVLQKTGGLFGLAVGLMQLFSDYKEDLKPLLDTL 180 GLFFQIRDDYANLHSKEYSENKSFCEDLTEGKFSFPTIHAIWSRPESTQVQNILRQRTEN 240 IDIKKYCVQYLEDVGSFAYTRHTLRELEAKAYKQIEACGGNPSLVALVKHLSKMFTEENK 300 SEQIDNO:25 ArtificialSequence atggcaagattctattttcttaacgcactattgatggttatctcattacaatcaactaca 60 gccttcactccagctaaacttgcttatccaacaacaacaacagctctaaatgtcgcctcc 120 gccgaaacttctttcagtctagatgaatacttggcctctaagataggacctatagagtct 180 gccttggaagcatcagtcaaatccagaattccacagaccgataagatctgcgaatctatg 240 gcctactctttgatggcaggaggcaagagaattagaccagtgttgtgtatcgctgcatgt 300 gagatgttcggtggatcccaagatgtcgctatgcctactgctgtggcattagaaatgata 360 cacacaatgtctttgattcatgatgatttgccatccatggataacgatgacttgagaaga 420 ggtaaaccaacaaaccatgtcgttttcggcgaagatgtagctattcttgcaggtgactct 480 ttattgtcaacttccttcgagcacgtcgctagagaaacaaaaggagtgtcagcagaaaag 540 atcgtggatgttatcgctagattaggcaaatctgttggtgccgagggccttgctggcggt 600 caagttatggacttagaatgtgaagctaaaccaggtaccacattagacgacttgaaatgg 660 attcatatccataaaaccgctacattgttacaagttgctgtagcttctggtgcagttcta 720 ggtggtgcaactcctgaagaggttgctgcatgcgagttgtttgctatgaatataggtctt 780 gcctttcaagttgccgacgatatccttgatgtaaccgcttcatcagaagatttgggtaaa 840 actgcaggcaaagatgaagctactgataagacaacttacccaaagttattaggattagaa 900 gagagtaaggcatacgcaagacaactaatcgatgaagccaaggaaagtttggctcctttt 960 ggagatagagctgcccctttattggccattgcagatttcattattgatagaaagaattga 1020 SEQIDNO:26 Thalassiosirapseudonana MARFYFLNALLMVISLQSTTAFTPAKLAYPTTTTALNVASAETSFSLDEYLASKIGPIES 60 ALEASVKSRIPQTDKICESMAYSLMAGGKRIRPVLCIAACEMFGGSQDVAMPTAVALEMI 120 HTMSLIHDDLPSMDNDDLRRGKPTNHVVFGEDVAILAGDSLLSTSFEHVARETKGVSAEK 180 IVDVIARLGKSVGAEGLAGGQVMDLECEAKPGTTLDDLKWIHIHKTATLLQVAVASGAVL 240 GGATPEEVAACELFAMNIGLAFQVADDILDVTASSEDLGKTAGKDEATDKTTYPKLLGLE 300 ESKAYARQLIDEAKESLAPFGDRAAPLLAIADFIIDRKN 339 SEQIDNO:27 ArtificialSequence atgcacttagcaccacgtagagtccctagaggtagaagatcaccacctgacagagttcct 60 gaaagacaaggtgccttgggtagaagacgtggagctggctctactggctgtgcccgtgct 120 gctgctggtgttcaccgtagaagaggaggaggcgaggctgatccatcagctgctgtgcat 180 agaggctggcaagccggtggtggcaccggtttgcctgatgaggtggtgtctaccgcagcc 240 gccttagaaatgtttcatgcttttgctttaatccatgatgatatcatggatgatagtgca 300 actagaagaggctccccaactgttcacagagccctagctgatcgtttaggcgctgctctg 360 gacccagatcaggccggtcaactaggagtttctactgctatcttggttggagatctggct 420 ttgacatggtccgatgaattgttatacgctccattgactccacatagactggcagcagta 480 ctaccattggtaacagctatgagagctgaaaccgttcatggccaatatcttgatataact 540 agtgctagaagacctgggaccgatacttctcttgcattgagaatagccagatataagaca 600 gcagcttacacaatggaacgtccactgcacattggtgcagccctggctggggcaagacca 660 gaactattagcagggctttcagcatacgccttgccagctggagaagccttccaattggca 720 gatgacctgctaggcgtcttcggtgatccaagacgtacagggaaacctgacctagatgat 780 cttagaggtggaaagcatactgtcttagtcgccttggcaagagaacatgccactccagaa 840 cagagacacacattggatacattattgggtacaccaggtcttgatagacaaggcgcttca 900 agactaagatgcgtattggtagcaactggtgcaagagccgaagccgaaagacttattaca 960 gagagaagagatcaagcattaactgcattgaacgcattaacactgccacctcctttagct 1020 gaggcattagcaagattgacattagggtctacagctcatcctgcctaa 1068 SEQIDNO:28 Streptomycesclavuligerus MHLAPRRVPRGRRSPPDRVPERQGALGRRRGAGSTGCARAAAGVHRRRGGGEADPSAAVH 60 RGWQAGGGTGLPDEVVSTAAALEMFHAFALIHDDIMDDSATRRGSPTVHRALADRLGAAL 120 DPDQAGQLGVSTAILVGDLALTWSDELLYAPLTPHRLAAVLPLVTAMRAETVHGQYLDIT 180 SARRPGTDTSLALRIARYKTAAYTMERPLHIGAALAGARPELLAGLSAYALPAGEAFQLA 240 DDLLGVFGDPRRTGKPDLDDLRGGKHTVLVALAREHATPEQRHTLDTLLGTPGLDRQGAS 300 RLRCVLVATGARAEAERLITERRDQALTALNALTLPPPLAEALARLTLGSTAHPA 355 SEQIDNO:29 ArtificialSequence atgtcatatttcgataactacttcaatgagatagttaattccgtgaacgacatcattaag 60 tcttacatctctggcgacgtaccaaaactatacgaagcctcctaccatttgtttacatca 120 ggaggaaagagactaagaccattgatccttacaatttcttctgatcttttcggtggacag 180 agagaaagagcatactatgctggcgcagcaatcgaagttttgcacacattcactttggtt 240 cacgatgatatcatggatcaagataacattcgtagaggtcttcctactgtacatgtcaag 300 tatggcctacctttggccattttagctggtgacttattgcatgcaaaagcctttcaattg 360 ttgactcaggcattgagaggtctaccatctgaaactatcatcaaggcgtttgatatcttt 420 acaagatctatcattatcatatcagaaggtcaagctgtcgatatggaattcgaagataga 480 attgatatcaaggaacaagagtatttggatatgatatctcgtaaaaccgctgccttattc 540 tcagcttcttcttccattggggcgttgatagctggagctaatgataacgatgtgagatta 600 atgtccgatttcggtacaaatcttgggatcgcatttcaaattgtagatgatatacttggt 660 ttaacagctgatgaaaaagagctaggaaaacctgttttcagtgatatcagagaaggtaaa 720 aagaccatattagtcattaagactttagaattgtgtaaggaagacgagaaaaagattgtg 780 ttaaaagcgctaggcaacaagtcagcatcaaaggaagagttgatgagttctgctgacata 840 atcaaaaagtactcattggattacgcctacaacttagctgagaaatactacaaaaacgcc 900 atcgattctctaaatcaagtttcaagtaaaagtgatattccagggaaggcattgaaatat 960 cttgctgaattcaccatcagaagacgtaagtaa 993 SEQIDNO:30 Sulfolobusacidocaldarius MSYFDNYFNEIVNSVNDIIKSYISGDVPKLYEASYHLFTSGGKRLRPLILTISSDLFGGQ 60 RERAYYAGAAIEVLHTFTLVHDDIMDQDNIRRGLPTVHVKYGLPLAILAGDLLHAKAFQL 120 LTQALRGLPSETIIKAFDIFTRSIIIISEGQAVDMEFEDRIDIKEQEYLDMISRKTAALF 180 SASSSIGALIAGANDNDVRLMSDFGTNLGIAFQIVDDILGLTADEKELGKPVFSDIREGK 240 KTILVIKTLELCKEDEKKIVLKALGNKSASKEELMSSADIIKKYSLDYAYNLAEKYYKNA 300 IDSLNQVSSKSDIPGKALKYLAEFTIRRRK 330 SEQIDNO:31 ArtificialSequence atggtcgcacaaactttcaacctggatacctacttatcccaaagacaacaacaagttgaa 60 gaggccctaagtgctgctcttgtgccagcttatcctgagagaatatacgaagctatgaga 120 tactccctcctggcaggtggcaaaagattaagacctatcttatgtttagctgcttgcgaa 180 ttggcaggtggttctgttgaacaagccatgccaactgcgtgtgcacttgaaatgatccat 240 acaatgtcactaattcatgatgacctgccagccatggataacgatgatttcagaagagga 300 aagccaactaatcacaaggtgttcggggaagatatagccatcttagcgggtgatgcgctt 360 ttagcttacgcttttgaacatattgcttctcaaacaagaggagtaccacctcaattggtg 420 ctacaagttattgctagaatcggacacgccgttgctgcaacaggcctcgttggaggccaa 480 gtcgtagaccttgaatctgaaggtaaagctatttccttagaaacattggagtatattcac 540 tcacataagactggagccttgctggaagcatcagttgtctcaggcggtattctcgcaggg 600 gcagatgaagagcttttggccagattgtctcattacgctagagatataggcttggctttt 660 caaatcgtcgatgatatcctggatgttactgctacatctgaacagttggggaaaaccgct 720 ggtaaagaccaggcagccgcaaaggcaacttatccaagtctattgggtttagaagcctct 780 agacagaaagcggaagagttgattcaatctgctaaggaagccttaagaccttacggttca 840 caagcagagccactcctagcgctggcagacttcatcacacgtcgtcagcattaa 894 SEQIDNO:32 Synechococcussp. MVAQTFNLDTYLSQRQQQVEEALSAALVPAYPERIYEAMRYSLLAGGKRLRPILCLAACE 60 LAGGSVEQAMPTACALEMIHTMSLIHDDLPAMDNDDFRRGKPTNHKVFGEDIAILAGDAL 120 LAYAFEHIASQTRGVPPQLVLQVIARIGHAVAATGLVGGQVVDLESEGKAISLETLEYIH 180 SHKTGALLEASVVSGGILAGADEELLARLSHYARDIGLAFQIVDDILDVTATSEQLGKTA 240 GKDQAAAKATYPSLLGLEASRQKAEELIQSAKEALRPYGSQAEPLLALADFITRRQH 297 SEQIDNO:33 ArtificialSequence atgaaaaccgggtttatctcaccagcaacagtatttcatcacagaatctcaccagcgacc 60 actttcagacatcacttatcacctgctactacaaactctacaggcattgtcgccttaaga 120 gacatcaacttcagatgtaaagcagtttctaaagagtactctgatctgttgcagaaagat 180 gaggcttctttcacaaaatgggacgatgacaaggtgaaagatcatcttgataccaacaaa 240 aacttatacccaaatgatgagattaaggaatttgttgaatcagtaaaggctatgttcggt 300 agtatgaatgacggggagataaacgtctctgcatacgatactgcatgggttgctttggtt 360 caagatgtcgatggatcaggtagtcctcagttcccttcttctttagaatggattgccaac 420 aatcaattgtcagatggatcatggggagatcatttgctgttctcagctcacgatagaatc 480 atcaacacattagcatgcgttattgcacttacaagttggaatgttcatccttctaagtgt 540 gaaaaaggtttgaattttctgagagaaaacatttgcaaattagaagatgaaaacgcagaa 600 catatgccaattggttttgaagtaacattcccatcactaattgatatcgcgaaaaagttg 660 aacattgaagtacctgaggatactccagcacttaaagagatctacgcacgtagagatatc 720 aagttaactaagatcccaatggaagttcttcacaaggtacctactactttgttacattct 780 ttggaaggaatgcctgatttggagtgggaaaaactgttaaagctacaatgtaaagatggt 840 agtttcttgttttccccatctagtaccgcattcgccctaatgcaaacaaaagatgagaaa 900 tgcttacagtatctaacaaatatcgtcactaagttcaacggtggcgtgcctaatgtgtac 960 ccagtcgatttgtttgaacatatttgggttgttgatagactgcagagattggggattgcc 1020 agatacttcaaatcagagataaaagattgtgtagagtatatcaataagtactggaccaaa 1080 aatggaatttgttgggctagaaatactcacgttcaagatatcgatgatacagccatggga 1140 ttcagagtgttgagagcgcacggttatgacgtcactccagatgtttttagacaatttgaa 1200 aaagatggtaaattcgtttgctttgcagggcaatcaacacaagccgtgacaggaatgttt 1260 aacgtttacagagcctctcaaatgttgttcccaggggagagaattttggaagatgccaaa 1320 aagttctcttacaattacttaaaggaaaagcaaagtaccaacgaattgctggataaatgg 1380 ataatcgctaaagatctacctggtgaagttggttatgctctggatatcccatggtatgct 1440 tccttaccaagattggaaactcgttattaccttgaacaatacggcggtgaagatgatgtc 1500 tggataggcaagacattatacagaatgggttacgtgtccaataacacatatctagaaatg 1560 gcaaagctggattacaataactatgttgcagtccttcaattagaatggtacacaatacaa 1620 caatggtacgtcgatattggtatagagaagttcgaatctgacaacatcaagtcagtcctg 1680 SEQIDNO:34 Steviarebaudiana MKTGFISPATVFHHRISPATTFRHHLSPATTNSTGIVALRDINFRCKAVSKEYSDLLQKD 60 EASFTKWDDDKVKDHLDTNKNLYPNDEIKEFVESVKAMFGSMNDGEINVSAYDTAWVALV 120 QDVDGSGSPQFPSSLEWIANNQLSDGSWGDHLLFSAHDRIINTLACVIALTSWNVHPSKC 180 EKGLNFLRENICKLEDENAEHMPIGFEVTFPSLIDIAKKLNIEVPEDTPALKEIYARRDI 240 KLTKIPMEVLHKVPTTLLHSLEGMPDLEWEKLLKLQCKDGSFLFSPSSTAFALMQTKDEK 300 CLQYLTNIVTKFNGGVPNVYPVDLFEHIWVVDRLQRLGIARYFKSEIKDCVEYINKYWTK 360 NGICWARNTHVQDIDDTAMGFRVLRAHGYDVTPDVFRQFEKDGKFVCFAGQSTQAVTGMF 420 NVYRASQMLFPGERILEDAKKFSYNYLKEKQSTNELLDKWIIAKDLPGEVGYALDIPWYA 480 SLPRLETRYYLEQYGGEDDVWIGKTLYRMGYVSNNTYLEMAKLDYNNYVAVLQLEWYTIQ 540 QWYVDIGIEKFESDNIKSVLVSYYLAAASIFEPERSKERIAWAKTTILVDKITSIFDSSQ 600 SSKEDITAFIDKFRNKSSSKKHSINGEPWHEVMVALKKTLHGFALDALMTHSQDIHPQLH 660 QAWEMWLTKLQDGVDVTAELMVQMINMTAGRWVSKELLTHPQYQRLSTVTNSVCHDITKL 720 HNFKENSTTVDSKVQELVQLVFSDTPDDLDQDMKQTFLTVMKTFYYKAWCDPNTINDHIS 780 KVFEIVI 787 SEQIDNO:35 ArtificialSequence atgcctgatgcacacgatgctccacctccacaaataagacagagaacactagtagatgag 60 gctacccaactgctaactgagtccgcagaagatgcatggggtgaagtcagtgtgtcagaa 120 tacgaaacagcaaggctagttgcccatgctacatggttaggtggacacgccacaagagtg 180 gccttccttctggagagacaacacgaagacgggtcatggggtccaccaggtggatatagg 240 ttagtccctacattatctgctgttcacgcattattgacatgtcttgcctctcctgctcag 300 gatcatggcgttccacatgatagacttttaagagctgttgacgcaggcttgactgccttg 360 agaagattggggacatctgactccccacctgatactatagcagttgagctggttatccca 420 tctttgctagagggcattcaacacttactggaccctgctcatcctcatagtagaccagcc 480 ttctctcaacatagaggctctcttgtttgtcctggtggactagatgggagaactctagga 540 gctttgagatcacacgccgcagcaggtacaccagtaccaggaaaagtctggcacgcttcc 600 gagactttgggcttgagtaccgaagctgcttctcacttgcaaccagcccaaggtataatc 660 ggtggctctgctgctgccacagcaacatggctaaccagggttgcaccatctcaacagtca 720 gattctgccagaagataccttgaggaattacaacacagatactctggcccagttccttcc 780 attacccctatcacatacttcgaaagagcatggttattgaacaattttgcagcagccggt 840 gttccttgtgaggctccagctgctttgttggattccttagaagcagcacttacaccacaa 900 ggtgctcctgctggagcaggattgcctccagatgctgatgatacagccgctgtgttgctt 960 gcattggcaacacatgggagaggtagaagaccagaagtactgatggattacaggactgac 1020 gggtatttccaatgctttattggggaaaggactccatcaatttcaacaaacgctcacgta 1080 ttggaaacattagggcatcatgtggcccaacatccacaagatagagccagatacggatca 1140 gccatggataccgcatcagcttggctgctggcagctcaaaagcaagatggctcttggtta 1200 gataaatggcatgcctcaccatactacgctactgtttgttgcacacaagccctagccgct 1260 catgcaagtcctgcaactgcaccagctagacagagagctgtcagatgggttttagccaca 1320 caaagatccgatggcggttggggtctatggcattcaactgttgaagagactgcttatgcc 1380 ttacagatcttggccccaccttctggtggtggcaatatcccagtccaacaagcacttact 1440 agaggcagagcaagattgtgtggagccttgccactgactcctttatggcatgataaggat 1500 ttgtatactccagtaagagtagtcagagctgccagagctgctgctctgtacactaccaga 1560 gatctattgttaccaccattgtaa 1584 SEQIDNO:36 Streptomycesclavuligerus MPDAHDAPPPQIRQRTLVDEATQLLTESAEDAWGEVSVSEYETARLVAHATWLGGHATRV 60 AFLLERQHEDGSWGPPGGYRLVPTLSAVHALLTCLASPAQDHGVPHDRLLRAVDAGLTAL 120 RRLGTSDSPPDTIAVELVIPSLLEGIQHLLDPAHPHSRPAFSQHRGSLVCPGGLDGRTLG 180 ALRSHAAAGTPVPGKVWHASETLGLSTEAASHLQPAQGIIGGSAAATATWLTRVAPSQQS 240 DSARRYLEELQHRYSGPVPSITPITYFERAWLLNNFAAAGVPCEAPAALLDSLEAALTPQ 300 GAPAGAGLPPDADDTAAVLLALATHGRGRRPEVLMDYRTDGYFQCFIGERTPSISTNAHV 360 LETLGHHVAQHPQDRARYGSAMDTASAWLLAAQKQDGSWLDKWHASPYYATVCCTQALAA 420 HASPATAPARQRAVRWVLATQRSDGGWGLWHSTVEETAYALQILAPPSGGGNIPVQQALT 480 RGRARLCGALPLTPLWHDKDLYTPVRVVRAARAAALYTTRDLLLPPL 527 SEQIDNO:37 ArtificialSequence atgaacgccctatccgaacacattttgtctgaattgagaagattattgtctgaaatgagt 60 gatggcggatctgttggtccatctgtgtatgatacggcccaggccctaagattccacggt 120 aacgtaacaggtagacaagatgcatatgcttggttgatcgcccagcaacaagcagatgga 180 ggttggggctctgccgactttccactctttagacatgctccaacatgggctgcacttctc 240 gcattacaaagagctgatccacttcctggcgcagcagacgcagttcagaccgcaacaaga 300 ttcttgcaaagacaaccagatccatacgctcatgccgttcctgaggatgcccctattggt 360 gctgaactgatcttgcctcagttttgtggagaggctgcttggttgttgggaggtgtggcc 420 ttccctagacacccagccctattaccattaagacaggcttgtttagtcaaactgggtgca 480 gtcgccatgttgccttcaggacacccattgctccactcctgggaggcatggggtacttct 540 ccaacaacagcctgtccagacgatgatggttctataggtatctcaccagcagctacagcc 600 gcctggagagcccaggctgtgaccagaggctcaactcctcaagtgggcagagctgacgca 660 tacttacaaatggcttcaagagcaacgagatcaggcatagaaggagtcttccctaatgtt 720 tggcctataaacgtattcgaaccatgctggtcactgtacactctccatcttgccggtctg 780 ttcgcccatccagcactggctgaggctgtaagagttatcgttgctcaacttgaagcaaga 840 ttgggagtgcatggcctcggaccagctttacattttgctgccgacgctgatgatactgca 900 gttgccttatgcgttctgcatttggctggcagagatcctgcagttgacgcattgagacat 960 tttgaaattggtgagctctttgttacattcccaggagagagaaatgctagtgtctctacg 1020 aacattcacgctcttcatgctttgagattgttaggtaaaccagctgccggagcaagtgca 1080 tacgtcgaagcaaatagaaatccacatggtttgtgggacaacgaaaaatggcacgtttca 1140 tggctttatccaactgcacacgccgttgcagctctagctcaaggcaagcctcaatggaga 1200 gatgaaagagcactagccgctctactacaagctcaaagagatgatggtggttggggagct 1260 ggtagaggatccactttcgaggaaaccgcctacgctcttttcgctttacacgttatggac 1320 ggatctgaggaagccacaggcagaagaagaatcgctcaagtcgtcgcaagagccttagaa 1380 tggatgctagctagacatgccgcacatggattaccacaaacaccactctggattggtaag 1440 gaattgtactgtcctactagagtcgtaagagtagctgagctagctggcctgtggttagca 1500 ttaagatggggtagaagagtattagctgaaggtgctggtgctgcaccttaa 1551 SEQIDNO:38 Bradyrhizobiumjaponicum MNALSEHILSELRRLLSEMSDGGSVGPSVYDTAQALRFHGNVTGRQDAYAWLIAQQQADG 60 GWGSADFPLFRHAPTWAALLALQRADPLPGAADAVQTATRFLQRQPDPYAHAVPEDAPIG 120 AELILPQFCGEAAWLLGGVAFPRHPALLPLRQACLVKLGAVAMLPSGHPLLHSWEAWGTS 180 PTTACPDDDGSIGISPAATAAWRAQAVTRGSTPQVGRADAYLQMASRATRSGIEGVFPNV 240 WPINVFEPCWSLYTLHLAGLFAHPALAEAVRVIVAQLEARLGVHGLGPALHFAADADDTA 300 VALCVLHLAGRDPAVDALRHFEIGELFVTFPGERNASVSTNIHALHALRLLGKPAAGASA 360 YVEANRNPHGLWDNEKWHVSWLYPTAHAVAALAQGKPQWRDERALAALLQAQRDDGGWGA 420 GRGSTFEETAYALFALHVMDGSEEATGRRRIAQVVARALEWMLARHAAHGLPQTPLWIGK 480 ELYCPTRVVRVAELAGLWLALRWGRRVLAEGAGAAP 516 SEQIDNO:39 ArtificialSequence atggttttgtcttcttcttgtactacagtaccacacttatcttcattagctgtcgtgcaa 60 cttggtccttggagcagtaggattaaaaagaaaaccgatactgttgcagtaccagccgct 120 gcaggaaggtggagaagggccttggctagagcacagcacacatcagaatccgcagctgtc 180 gcaaagggcagcagtttgacccctatagtgagaactgacgctgagtcaaggagaacaaga 240 tggccaaccgatgacgatgacgccgaacctttagtggatgagatcagggcaatgcttact 300 tccatgtctgatggtgacatttccgtgagcgcatacgatacagcctgggtcggattggtt 360 ccaagattagacggcggtgaaggtcctcaatttccagcagctgtgagatggataagaaat 420 aaccagttgcctgacggaagttggggcgatgccgcattattctctgcctatgacaggctt 480 atcaatacccttgcctgcgttgtaactttgacaaggtggtccctagaaccagagatgaga 540 ggtagaggactatcttttttgggtaggaacatgtggaaattagcaactgaagatgaagag 600 tcaatgcctattggcttcgaattagcatttccatctttgatagagcttgctaagagccta 660 ggtgtccatgacttcccttatgatcaccaggccctacaaggaatctactcttcaagagag 720 atcaaaatgaagaggattccaaaagaagtgatgcataccgttccaacatcaatattgcac 780 agtttggagggtatgcctggcctagattgggctaaactacttaaactacagagcagcgac 840 ggaagttttttgttctcaccagctgccactgcatatgctttaatgaataccggagatgac 900 aggtgttttagctacatcgatagaacagtaaagaaattcaacggcggcgtccctaatgtt 960 tatccagtggatctatttgaacatatttgggccgttgatagacttgaaagattaggaatc 1020 tccaggtacttccaaaaggagatcgaacaatgcatggattatgtaaacaggcattggact 1080 gaggacggtatttgttgggcaaggaactctgatgtcaaagaggtggacgacacagctatg 1140 gcctttagacttcttaggttgcacggctacagcgtcagtcctgatgtgtttaaaaacttc 1200 gaaaaggacggtgaatttttcgcatttgtcggacagtctaatcaagctgttaccggtatg 1260 tacaacttaaacagagcaagccagatatccttcccaggcgaggatgtgcttcatagagct 1320 ggtgccttctcatatgagttcttgaggagaaaagaagcagagggagctttgagggacaag 1380 tggatcatttctaaagatctacctggtgaagttgtgtatactttggattttccatggtac 1440 ggcaacttacctagagtcgaggccagagactacctagagcaatacggaggtggtgatgac 1500 gtttggattggcaagacattgtataggatgccacttgtaaacaatgatgtatatttggaa 1560 ttggcaagaatggatttcaaccactgccaggctttgcatcagttagagtggcaaggacta 1620 aaaagatggtatactgaaaataggttgatggactttggtgtcgcccaagaagatgccctt 1680 agagcttattttcttgcagccgcatctgtttacgagccttgtagagctgccgagaggctt 1740 gcatgggctagagccgcaatactagctaacgccgtgagcacccacttaagaaatagccca 1800 tcattcagagaaaggttagagcattctcttaggtgtagacctagtgaagagacagatggc 1860 tcctggtttaactcctcaagtggctctgatgcagttttagtaaaggctgtcttaagactt 1920 actgattcattagccagggaagcacagccaatccatggaggtgacccagaagatattata 1980 cacaagttgttaagatctgcttgggccgagtgggttagggaaaaggcagacgctgccgat 2040 agcgtgtgcaatggtagttctgcagtagaacaagagggatcaagaatggtccatgataaa 2100 cagacctgtctattattggctagaatgatcgaaatttctgccggtagggcagctggtgaa 2160 gcagccagtgaggacggcgatagaagaataattcaattaacaggctccatctgcgacagt 2220 cttaagcaaaaaatgctagtttcacaggaccctgaaaaaaatgaagagatgatgtctcac 2280 gtggatgacgaattgaagttgaggattagagagttcgttcaatatttgcttagactaggt 2340 gaaaaaaagactggatctagcgaaaccaggcaaacatttttaagtatagtgaaatcatgt 2400 tactatgctgctcattgcccacctcatgtcgttgatagacacattagtagagtgattttc 2460 gagccagtaagtgccgcaaagtaaccgcgg 2490 SEQIDNO:40 Zeamays MVLSSSCTTVPHLSSLAVVQLGPWSSRIKKKTDTVAVPAAAGRWRRALARAQHTSESAAV 60 AKGSSLTPIVRTDAESRRTRWPTDDDDAEPLVDEIRAMLTSMSDGDISVSAYDTAWVGLV 120 PRLDGGEGPQFPAAVRWIRNNQLPDGSWGDAALFSAYDRLINTLACVVTLTRWSLEPEMR 180 GRGLSFLGRNMWKLATEDEESMPIGFELAFPSLIELAKSLGVHDFPYDHQALQGIYSSRE 240 IKMKRIPKEVMHTVPTSILHSLEGMPGLDWAKLLKLQSSDGSFLFSPAATAYALMNTGDD 300 RCFSYIDRTVKKFNGGVPNVYPVDLFEHIWAVDRLERLGISRYFQKEIEQCMDYVNRHWT 360 EDGICWARNSDVKEVDDTAMAFRLLRLHGYSVSPDVFKNFEKDGEFFAFVGQSNQAVTGM 420 YNLNRASQISFPGEDVLHRAGAFSYEFLRRKEAEGALRDKWIISKDLPGEVVYTLDFPWY 480 GNLPRVEARDYLEQYGGGDDVWIGKTLYRMPLVNNDVYLELARMDFNHCQALHQLEWQGL 540 KRWYTENRLMDFGVAQEDALRAYFLAAASVYEPCRAAERLAWARAAILANAVSTHLRNSP 600 SFRERLEHSLRCRPSEETDGSWFNSSSGSDAVLVKAVLRLTDSLAREAQPIHGGDPEDII 660 HKLLRSAWAEWVREKADAADSVCNGSSAVEQEGSRMVHDKQTCLLLARMIEISAGRAAGE 720 AASEDGDRRIIQLTGSICDSLKQKMLVSQDPEKNEEMMSHVDDELKLRIREFVQYLLRLG 780 EKKTGSSETRQTFLSIVKSCYYAAHCPPHVVDRHISRVIFEPVSAAK 827 SEQIDNO:41 ArtificialSequence cttcttcactaaatacttagacagagaaaacagagctttttaaagccatgtctcttcagt 60 atcatgttctaaactccattccaagtacaacctttctcagttctactaaaacaacaatat 120 cttcttctttccttaccatctcaggatctcctctcaatgtcgctagagacaaatccagaa 180 gcggttccatacattgttcaaagcttcgaactcaagaatacattaattctcaagaggttc 240 aacatgatttgcctctaatacatgagtggcaacagcttcaaggagaagatgctcctcaga 300 ttagtgttggaagtaatagtaatgcattcaaagaagcagtgaagagtgtgaaaacgatct 360 tgagaaacctaacggacggggaaattacgatatcggcttacgatacagcttgggttgcat 420 tgatcgatgccggagataaaactccggcgtttccctccgccgtgaaatggatcgccgaga 480 accaactttccgatggttcttggggagatgcgtatctcttctcttatcatgatcgtctca 540 tcaatacccttgcatgcgtcgttgctctaagatcatggaatctctttcctcatcaatgca 600 acaaaggaatcacgtttttccgggaaaatattgggaagctagaagacgaaaatgatgagc 660 atatgccaatcggattcgaagtagcattcccatcgttgcttgagatagctcgaggaataa 720 acattgatgtaccgtacgattctccggtcttaaaagatatatacgccaagaaagagctaa 780 agcttacaaggataccaaaagagataatgcacaagataccaacaacattgttgcatagtt 840 tggaggggatgcgtgatttagattgggaaaagctcttgaaacttcaatctcaagacggat 900 ctttcctcttctctccttcctctaccgcttttgcattcatgcagacccgagacagtaact 960 gcctcgagtatttgcgaaatgccgtcaaacgtttcaatggaggagttcccaatgtctttc 1020 ccgtggatcttttcgagcacatatggatagtggatcggttacaacgtttagggatatcga 1080 gatactttgaagaagagattaaagagtgtcttgactatgtccacagatattggaccgaca 1140 atggcatatgttgggctagatgttcccatgtccaagacatcgatgatacagccatggcat 1200 ttaggctcttaagacaacatggataccaagtgtccgcagatgtattcaagaactttgaga 1260 aagagggagagtttttctgctttgtggggcaatcaaaccaagcagtaaccggtatgttca 1320 acctataccgggcatcacaattggcgtttccaagggaagagatattgaaaaacgccaaag 1380 agttttcttataattatctgctagaaaaacgggagagagaggagttgattgataagtgga 1440 ttataatgaaagacttacctggcgagattgggtttgcgttagagattccatggtacgcaa 1500 gcttgcctcgagtagagacgagattctatattgatcaatatggtggagaaaacgacgttt 1560 ggattggcaagactctttataggatgccatacgtgaacaataatggatatctggaattag 1620 caaaacaagattacaacaattgccaagctcagcatcagctcgaatgggacatattccaaa 1680 agtggtatgaagaaaataggttaagtgagtggggtgtgcgcagaagtgagcttctcgagt 1740 gttactacttagcggctgcaactatatttgaatcagaaaggtcacatgagagaatggttt 1800 gggctaagtcaagtgtattggttaaagccatttcttcttcttttggggaatcctctgact 1860 ccagaagaagcttctccgatcagtttcatgaatacattgccaatgctcgacgaagtgatc 1920 atcactttaatgacaggaacatgagattggaccgaccaggatcggttcaggccagtcggc 1980 ttgccggagtgttaatcgggactttgaatcaaatgtcttttgaccttttcatgtctcatg 2040 gccgtgacgttaacaatctcctctatctatcgtggggagattggatggaaaaatggaaac 2100 tatatggagatgaaggagaaggagagctcatggtgaagatgataattctaatgaagaaca 2160 atgacctaactaacttcttcacccacactcacttcgttcgtctcgcggaaatcatcaatc 2220 gaatctgtcttcctcgccaatacttaaaggcaaggagaaacgatgagaaggagaagacaa 2280 taaagagtatggagaaggagatggggaaaatggttgagttagcattgtcggagagtgaca 2340 catttcgtgacgtcagcatcacgtttcttgatgtagcaaaagcattttactactttgctt 2400 tatgtggcgatcatctccaaactcacatctccaaagtcttgtttcaaaaagtctagtaac 2460 ctcatcatcatcatcgatccattaacaatcagtggatcgatgtatccatagatgcgtgaa 2520 taatatttcatgtagagaaggagaacaaattagatcatgtagggttatca 2570 SEQIDNO:42 Arabidopsisthaliana MSLQYHVLNSIPSTTFLSSTKTTISSSFLTISGSPLNVARDKSRSGSIHCSKLRTQEYIN 60 SQEVQHDLPLIHEWQQLQGEDAPQISVGSNSNAFKEAVKSVKTILRNLTDGEITISAYDT 120 AWVALIDAGDKTPAFPSAVKWIAENQLSDGSWGDAYLFSYHDRLINTLACVVALRSWNLF 180 PHQCNKGITFFRENIGKLEDENDEHMPIGFEVAFPSLLEIARGINIDVPYDSPVLKDIYA 240 KKELKLTRIPKEIMHKIPTTLLHSLEGMRDLDWEKLLKLQSQDGSFLFSPSSTAFAFMQT 300 RDSNCLEYLRNAVKRFNGGVPNVFPVDLFEHIWIVDRLQRLGISRYFEEEIKECLDYVHR 360 YWTDNGICWARCSHVQDIDDTAMAFRLLRQHGYQVSADVFKNFEKEGEFFCFVGQSNQAV 420 TGMFNLYRASQLAFPREEILKNAKEFSYNYLLEKREREELIDKWIIMKDLPGEIGFALEI 480 PWYASLPRVETRFYIDQYGGENDVWIGKTLYRMPYVNNNGYLELAKQDYNNCQAQHQLEW 540 DIFQKWYEENRLSEWGVRRSELLECYYLAAATIFESERSHERMVWAKSSVLVKAISSSFG 600 ESSDSRRSFSDQFHEYIANARRSDHHFNDRNMRLDRPGSVQASRLAGVLIGTLNQMSFDL 660 FMSHGRDVNNLLYLSWGDWMEKWKLYGDEGEGELMVKMIILMKNNDLTNFFTHTHFVRLA 720 EIINRICLPRQYLKARRNDEKEKTIKSMEKEMGKMVELALSESDTFRDVSITFLDVAKAF 780 YYFALCGDHLQTHISKVLFQKV 802 SEQIDNO:43 ArtificialSequence atgaatttgagtttgtgtatagcatctccactattgaccaaatctaatagaccagctgct 60 ttatcagcaattcatacagctagtacatcccatggtggccaaaccaaccctacgaatctg 120 ataatcgatacgaccaaggagagaatacaaaaacaattcaaaaatgttgaaatttcagtt 180 tcttcttatgatactgcgtgggttgccatggttccatcacctaattctccaaagtctcca 240 tgtttcccagaatgtttgaattggctgattaacaaccagttgaatgatggatcttggggt 300 ttagtcaatcacacgcacaatcacaaccatccacttttgaaagattctttatcctcaact 360 ttggcttgcatcgtggccctaaagagatggaacgtaggtgaggatcagattaacaagggg 420 cttagtttcattgaatctaacttggcttccgcgactgaaaaatctcaaccatctccaata 480 ggattcgatatcatctttccaggtctgttagagtacgccaaaaatctagatatcaactta 540 ctgtctaagcaaactgatttctcactaatgttacacaagagagaattagaacaaaagaga 600 tgtcattcaaacgaaatggatggttacctagcttatatctctgaaggtcttggtaatctt 660 tacgattggaatatggtgaaaaagtaccagatgaaaaatggctcagttttcaattcccct 720 tctgcaactgcggcagcattcattaaccatcaaaatccaggatgcctgaactatttgaat 780 tcactactagacaaattcggcaacgcagttccaactgtataccctcacgatttgtttatc 840 agattgagtatggtggatacaattgaaagacttggtatatcccaccactttagagtcgag 900 atcaaaaatgttttggatgagacataccgttgttgggtggagagagatgaacaaatcttt 960 atggatgttgtgacgtgcgcgttggcctttagattgttgcgtattaacggttacgaagtt 1020 agtccagatccacttgccgaaattacaaacgaattagctttaaaggatgaatacgccgct 1080 cttgaaacatatcatgcgtcacatatcctttaccaagaggacttatcatctggaaaacaa 1140 attcttaaatctgctgatttcctgaaggaaatcatatccactgatagtaatagactgtcc 1200 aaactgatccataaagaggttgaaaatgcacttaagttccctattaacaccggcttagaa 1260 cgtattaacacaagacgtaacatccagctttacaacgtagacaatactagaatcttgaaa 1320 accacttaccattcttccaacatatcaaacactgattacctaagattagctgttgaagat 1380 ttctacacatgtcagtctatctatagagaagagctgaaaggattagagagatgggtcgtt 1440 gagaataagctagatcaattgaaatttgccagacaaaagacagcttattgttacttctca 1500 gttgccgccactttatcaagtccagaattgtcagatgcacgtatttcttgggctaaaaac 1560 ggaattttgacaactgttgttgatgatttctttgatattggcgggacaatcgacgaattg 1620 acaaacctgattcaatgcgttgaaaagtggaatgtcgatgtcgataaagactgttgctca 1680 gaacatgttagaatactgttcttggctctgaaagatgctatctgttggatcggggatgag 1740 gctttcaaatggcaagctagagatgtgacgtctcacgtcattcaaacctggctagaactg 1800 atgaactctatgttgagagaagcaatttggactagagatgcatacgttcctacattaaac 1860 gagtatatggaaaacgcttatgtctcctttgctttgggtcctatcgttaagcctgccata 1920 tactttgtaggaccaaagctatccgaggaaatcgtcgaatcatcagaataccataacttg 1980 ttcaagttaatgtccacacaaggcagattacttaatgatattcattctttcaaaagagag 2040 tttaaggaaggaaagttaaatgctgttgctctgcatctttctaatggcgaaagtggtaaa 2100 gtcgaagaggaagtagttgaggaaatgatgatgatgatcaaaaacaagagaaaggagttg 2160 atgaaactaatcttcgaagagaacggttcaattgttcctagagcatgtaaggatgcattt 2220 tggaacatgtgtcatgtgctaaactttttctacgcaaacgacgatggttttactgggaac 2280 acaatactagatacagtaaaagacatcatatacaaccctttggtcttagtaaacgaaaac 2340 gaggagcaaagataa 2355 SEQIDNO:44 Steviarebaudiana MNLSLCIASPLLTKSNRPAALSAIHTASTSHGGQTNPTNLIIDTTKERIQKQFKNVEISV 60 SSYDTAWVAMVPSPNSPKSPCFPECLNWLINNQLNDGSWGLVNHTHNHNHPLLKDSLSST 120 LACIVALKRWNVGEDQINKGLSFIESNLASATEKSQPSPIGFDIIFPGLLEYAKNLDINL 180 LSKQTDFSLMLHKRELEQKRCHSNEMDGYLAYISEGLGNLYDWNMVKKYQMKNGSVFNSP 240 SATAAAFINHQNPGCLNYLNSLLDKFGNAVPTVYPHDLFIRLSMVDTIERLGISHHFRVE 300 IKNVLDETYRCWVERDEQIFMDVVTCALAFRLLRINGYEVSPDPLAEITNELALKDEYAA 360 LETYHASHILYQEDLSSGKQILKSADFLKEIISTDSNRLSKLIHKEVENALKFPINTGLE 420 RINTRRNIQLYNVDNTRILKTTYHSSNISNTDYLRLAVEDFYTCQSIYREELKGLERWVV 480 ENKLDQLKFARQKTAYCYFSVAATLSSPELSDARISWAKNGILTTVVDDFFDIGGTIDEL 540 TNLIQCVEKWNVDVDKDCCSEHVRILFLALKDAICWIGDEAFKWQARDVTSHVIQTWLEL 600 MNSMLREAIWTRDAYVPTLNEYMENAYVSFALGPIVKPAIYFVGPKLSEEIVESSEYHNL 660 FKLMSTQGRLLNDIHSFKREFKEGKLNAVALHLSNGESGKVEEEVVEEMMMMIKNKRKEL 720 MKLIFEENGSIVPRACKDAFWNMCHVLNFFYANDDGFTGNTILDTVKDIIYNPLVLVNEN 780 EEQR 784 SEQIDNO:45 ArtificialSequence atgaatctgtccctttgtatagctagtccactgttgacaaaatcttctagaccaactgct 60 ctttctgcaattcatactgccagtactagtcatggaggtcaaacaaacccaacaaatttg 120 ataatcgatactactaaggagagaatccaaaagctattcaaaaatgttgaaatctcagta 180 tcatcttatgacaccgcatgggttgcaatggtgccatcacctaattccccaaaaagtcca 240 tgttttccagagtgcttgaattggttaatcaataatcagttaaacgatggttcttggggt 300 ttagtcaaccacactcataaccacaatcatccattattgaaggactctttatcatcaaca 360 ttagcctgtattgttgcattgaaaagatggaatgtaggtgaagatcaaatcaacaagggt 420 ttatcattcatagaatccaatctagcttctgctaccgacaaatcacaaccatctccaatc 480 gggttcgacataatcttccctggtttgctggagtatgccaaaaaccttgatatcaactta 540 ctgtctaaacaaacagatttctctttgatgctacacaaaagagagttagagcagaaaaga 600 tgccattctaacgaaattgacgggtacttagcatatatctcagaaggtttgggtaatttg 660 tatgactggaacatggtcaaaaagtatcagatgaaaaatggatccgtattcaattctcct 720 tctgcaactgccgcagcattcattaatcatcaaaaccctgggtgtcttaactacttgaac 780 tcactattagataagtttggaaatgcagttccaacagtctatcctttggacttgtacatc 840 agattatctatggttgacactatagagagattaggtatttctcatcatttcagagttgag 900 atcaaaaatgttttggacgagacatacagatgttgggtcgaaagagatgagcaaatcttt 960 atggatgtcgtgacctgcgctctggcttttagattgctaaggatacacggatacaaagta 1020 tctcctgatcaactggctgagattacaaacgaactggctttcaaagacgaatacgccgca 1080 ttagaaacataccatgcatcccaaatactttaccaggaagacctaagttcaggaaaacaa 1140 atcttgaagtctgcagatttcctgaaaggcattctgtctacagatagtaataggttgtct 1200 aaattgatacacaaggaagtagaaaacgcactaaagtttcctattaacactggtttagag 1260 agaatcaatactaggagaaacattcagctgtacaacgtagataatacaaggattcttaag 1320 accacctaccatagttcaaacatttccaacacctattacttaagattagctgtcgaagac 1380 ttttacacttgtcaatcaatctacagagaggagttaaagggcctagaaagatgggtagtt 1440 caaaacaagttggatcaactgaagtttgctagacagaagacagcatactgttatttctct 1500 gttgctgctaccctttcatccccagaattgtctgatgccagaataagttgggccaaaaat 1560 ggtattcttacaactgtagtcgatgatttctttgatattggaggtactattgatgaactg 1620 acaaatcttattcaatgtgttgaaaagtggaacgtggatgtagataaggattgctgcagt 1680 gaacatgtgagaatacttttcctggctctaaaagatgcaatatgttggattggcgacgag 1740 gccttcaagtggcaagctagagatgttacatctcatgtcatccaaacttggcttgaactg 1800 atgaactcaatgctaagagaagcaatctggacaagagatgcatacgttccaacattgaac 1860 gaatacatggaaaacgcttacgtctcatttgccttgggtcctattgttaagccagccata 1920 tactttgttgggccaaagttatccgaagagattgttgagtcttccgaatatcataaccta 1980 ttcaagttaatgtcaacacaaggcagacttctgaacgatatccactccttcaaaagagaa 2040 ttcaaggaaggtaagctaaacgctgttgctttgcacttgtctaatggtgaatctggcaaa 2100 gtggaagaggaagtcgttgaggaaatgatgatgatgatcaaaaacaagagaaaggaattg 2160 atgaaattgattttcgaggaaaatggttcaatcgtacctagagcttgtaaagatgctttt 2220 tggaatatgtgccatgttcttaacttcttttacgctaatgatgatggcttcactggaaat 2280 acaatattggatacagttaaagatatcatctacaacccacttgttttggtcaatgagaac 2340 gaggaacaaagataa 2355 SEQIDNO:46 Steviarebaudiana MNLSLCIASPLLTKSSRPTALSAIHTASTSHGGQTNPTNLIIDTTKERIQKLFKNVEISV 60 SSYDTAWVAMVPSPNSPKSPCFPECLNWLINNQLNDGSWGLVNHTHNHNHPLLKDSLSST 120 LACIVALKRWNVGEDQINKGLSFIESNLASATDKSQPSPIGFDIIFPGLLEYAKNLDINL 180 LSKQTDFSLMLHKRELEQKRCHSNEIDGYLAYISEGLGNLYDWNMVKKYQMKNGSVFNSP 240 SATAAAFINHQNPGCLNYLNSLLDKFGNAVPTVYPLDLYIRLSMVDTIERLGISHHFRVE 300 IKNVLDETYRCWVERDEQIFMDVVTCALAFRLLRIHGYKVSPDQLAEITNELAFKDEYAA 360 LETYHASQILYQEDLSSGKQILKSADFLKGILSTDSNRLSKLIHKEVENALKFPINTGLE 420 RINTRRNIQLYNVDNTRILKTTYHSSNISNTYYLRLAVEDFYTCQSIYREELKGLERWVV 480 QNKLDQLKFARQKTAYCYFSVAATLSSPELSDARISWAKNGILTTVVDDFFDIGGTIDEL 540 TNLIQCVEKWNVDVDKDCCSEHVRILFLALKDAICWIGDEAFKWQARDVTSHVIQTWLEL 600 MNSMLREAIWTRDAYVPTLNEYMENAYVSFALGPIVKPAIYFVGPKLSEEIVESSEYHNL 660 FKLMSTQGRLLNDIHSFKREFKEGKLNAVALHLSNGESGKVEEEVVEEMMMMIKNKRKEL 720 MKLIFEENGSIVPRACKDAFWNMCHVLNFFYANDDGFTGNTILDTVKDIIYNPLVLVNEN 780 EEQR 784 SEQIDNO:47 ArtificialSequence atggctatgccagtgaagctaacacctgcgtcattatccttaaaagctgtgtgctgcaga 60 ttctcatccggtggccatgctttgagattcgggagtagtctgccatgttggagaaggacc 120 cctacccaaagatctacttcttcctctactactagaccagctgccgaagtgtcatcaggt 180 aagagtaaacaacatgatcaggaagctagtgaagcgactatcagacaacaattacaactt 240 gtggatgtcctggagaatatgggaatatccagacattttgctgcagagataaagtgcata 300 ctagacagaacttacagatcttggttacaaagacacgaggaaatcatgctggacactatg 360 acatgtgctatggcttttagaatcctaagattgaacggatacaacgtttcatcagatgaa 420 ctataccacgttgtagaggcatctggtctgcataattctttgggtgggtatcttaacgat 480 accagaacactacttgaattacacaaggcttcaacagttagtatctctgaggatgaatct 540 atcttagattcaattggctctagatccagaacattgcttagagaacaattggagtctggt 600 ggcgcactgagaaagccttctttattcaaagaggttgaacatgcactggatggacctttt 660 tacaccacacttgatagacttcatcataggtggaatattgaaaacttcaacattattgag 720 caacacatgttggagactccatacttatctaaccagcatacatcaagggatatcctagca 780 ttgtcaattagagatttttcctcctcacaattcacttatcaacaagagctacagcatctg 840 gagagttgggttaaggaatgtagattagatcaactacagttcgcaagacagaaattagcg 900 tacttttacctatcagccgcaggcaccatgttttctcctgagctttctgatgcgagaaca 960 ttatgggccaaaaacggggtgttgacaactattgttgatgatttctttgatgttgccggt 1020 tctaaagaggaattggaaaacttagtcatgctggtcgaaatgtgggatgaacatcacaaa 1080 gttgaattctattctgagcaggtcgaaatcatcttctcttccatctacgattctgtcaac 1140 caattgggtgagaaggcctctttggttcaagacagatcaattacaaaacaccttgttgaa 1200 atatggttagacttgttaaagtccatgatgacggaagttgaatggagactgtcaaaatac 1260 gtgcctacagaaaaggaatacatgattaatgcctctcttatcttcggcctaggtccaatc 1320 gttttaccagctttgtatttcgttggtccaaagatttcagaaagtatagtaaaggaccca 1380 gaatatgatgaattgttcaaactaatgtcaacatgtggtagattgttgaatgacgtgcaa 1440 acgttcgaaagagaatacaatgagggtaaactgaattctgtcagtctattggttcttcac 1500 ggaggcccaatgtctatttcagacgcaaagaggaaattacaaaagcctattgatacgtgt 1560 agaagagatcttctttctttggtccttagagaagagtctgtagtaccaagaccatgtaag 1620 gaactattctggaaaatgtgtaaagtgtgctatttcttttactcaacaactgatgggttt 1680 tctagtcaagtcgaaagagcaaaagaggtagacgctgtcataaatgagccactgaagttg 1740 caaggttctcatacactggtatctgatgtttaa 1773 SEQIDNO:48 Zeamays MAMPVKLTPASLSLKAVCCRFSSGGHALRFGSSLPCWRRTPTQRSTSSSTTRPAAEVSSG 60 KSKQHDQEASEATIRQQLQLVDVLENMGISRHFAAEIKCILDRTYRSWLQRHEEIMLDTM 120 TCAMAFRILRLNGYNVSSDELYHVVEASGLHNSLGGYLNDTRTLLELHKASTVSISEDES 180 ILDSIGSRSRTLLREQLESGGALRKPSLFKEVEHALDGPFYTTLDRLHHRWNIENFNIIE 240 QHMLETPYLSNQHTSRDILALSIRDFSSSQFTYQQELQHLESWVKECRLDQLQFARQKLA 300 YFYLSAAGTMFSPELSDARTLWAKNGVLTTIVDDFFDVAGSKEELENLVMLVEMWDEHHK 360 VEFYSEQVEIIFSSIYDSVNQLGEKASLVQDRSITKHLVEIWLDLLKSMMTEVEWRLSKY 420 VPTEKEYMINASLIFGLGPIVLPALYFVGPKISESIVKDPEYDELFKLMSTCGRLLNDVQ 480 TFEREYNEGKLNSVSLLVLHGGPMSISDAKRKLQKPIDTCRRDLLSLVLREESVVPRPCK 540 ELFWKMCKVCYFFYSTTDGFSSQVERAKEVDAVINEPLKLQGSHTLVSDV 590 SEQIDNO:49 ArtificialSequence atgcagaacttccatggtacaaaggaaaggatcaaaaagatgtttgacaagattgaattg 60 tccgtttcttcttatgatacagcctgggttgcaatggtcccatcccctgattgcccagaa 120 acaccttgttttccagaatgtactaaatggatcctagaaaatcagttgggtgatggtagt 180 tggtcacttcctcatggcaatccacttctagttaaagatgcattatcttccactcttgct 240 tgtattctggctcttaaaagatggggaatcggtgaggaacagattaacaaaggactgaga 300 ttcatagaactcaactctgctagtgtaaccgataacgaacaacacaaaccaattggattt 360 gacattatctttccaggtatgattgaatacgctatagacttagacctgaatctaccacta 420 aaaccaactgacattaactccatgttgcatcgtagagcccttgaattgacatcaggtgga 480 ggcaaaaatctagaaggtagaagagcttacttggcctacgtctctgaaggaatcggtaag 540 ctgcaagattgggaaatggctatgaaataccaacgtaaaaacggatctctgttcaatagt 600 ccatcaacaactgcagctgcattcatccatatacaagatgctgaatgcctccactatatt 660 cgttctcttctccagaaatttggaaacgcagtccctacaatataccctctcgatatctat 720 gccagactttcaatggtagatgccctggaacgtcttggtattgatagacatttcagaaag 780 gagagaaagttcgttctggatgaaacatacagattttggttgcaaggagaagaggagatt 840 ttctccgataacgcaacctgtgctttggccttcagaatattgagacttaatggttacgat 900 gtctctcttgaagatcacttctctaactctctgggcggttacttaaaggactcaggagca 960 gctttagaactgtacagagccctccaattgtcttacccagacgagtccctcctggaaaag 1020 caaaattctagaacttcttacttcttaaaacaaggtttatccaatgtctccctctgtggt 1080 gacagattgcgtaaaaacataattggagaggtgcatgatgctttaaacttttccgaccac 1140 gctaacttacaaagattagctattcgtagaaggattaagcattacgctactgacgataca 1200 aggattctaaaaacttcctacagatgctcaacaatcggtaaccaagattttctaaaactt 1260 gcagtggaagatttcaatatctgtcaatcaatacaaagagaggaattcaagcatattgaa 1320 agatgggtcgttgaaagacgtctagacaagttaaagttcgctagacaaaaagaggcctat 1380 tgctatttctcagccgcagcaacattgtttgcccctgaattgtctgatgctagaatgtct 1440 tgggccaaaaatggtgtattgacaactgtggttgatgatttcttcgatgtcggaggctct 1500 gaagaggaattagttaacttgatagaattgatcgagcgttgggatgtgaatggcagtgca 1560 gatttttgtagtgaggaagttgagattatctattctgctatccactcaactatctctgaa 1620 ataggtgataagtcatttggctggcaaggtagagatgtaaagtctcaagttatcaagatc 1680 tggctggacttattgaaatcaatgttaactgaagctcaatggtcttcaaacaagtctgtt 1740 cctaccctagatgagtatatgacaaccgcccatgtttcattcgcacttggtccaattgta 1800 cttccagccttatacttcgttggcccaaagttgtcagaagaggttgcaggtcatcctgaa 1860 ctactaaacctctacaaagtcacatctacttgtggcagactactgaatgattggagaagt 1920 tttaagagagaatccgaggaaggtaagctcaacgctattagtttatacatgatccactcc 1980 ggtggtgcttctacagaagaggaaacaatcgaacatttcaaaggtttgattgattctcag 2040 agaaggcaactgttacaattggtgttgcaagagaaggatagtatcatacctagaccatgt 2100 aaagatctattttggaatatgattaagttattacacactttctacatgaaagatgatggc 2160 ttcacctcaaatgagatgaggaatgtagttaaggcaatcattaacgaaccaatctcactg 2220 gatgaattatga 2232 SEQIDNO:50 Populustrichocarpa MSCIRPWFCPSSISATLTDPASKLVTGEFKTTSLNFHGTKERIKKMFDKIELSVSSYDTA 60 WVAMVPSPDCPETPCFPECTKWILENQLGDGSWSLPHGNPLLVKDALSSTLACILALKRW 120 GIGEEQINKGLRFIELNSASVTDNEQHKPIGFDIIFPGMIEYAKDLDLNLPLKPTDINSM 180 LHRRALELTSGGGKNLEGRRAYLAYVSEGIGKLQDWEMAMKYQRKNGSLFNSPSTTAAAF 240 IHIQDAECLHYIRSLLQKFGNAVPTIYPLDIYARLSMVDALERLGIDRHFRKERKFVLDE 300 TYRFWLQGEEEIFSDNATCALAFRILRLNGYDVSLEDHFSNSLGGYLKDSGAALELYRAL 360 QLSYPDESLLEKQNSRTSYFLKQGLSNVSLCGDRLRKNIIGEVHDALNFPDHANLQRLAI 420 RRRIKHYATDDTRILKTSYRCSTIGNQDFLKLAVEDFNICQSIQREEFKHIERWVVERRL 480 DKLKFARQKEAYCYFSAAATLFAPELSDARMSWAKNGVLTTVVDDFFDVGGSEEELVNLI 540 ELIERWDVNGSADFCSEEVEIIYSAIHSTISEIGDKSFGWQGRDVKSHVIKIWLDLLKSM 600 LTEAQWSSNKSVPTLDEYMTTAHVSFALGPIVLPALYFVGPKLSEEVAGHPELLNLYKVM 660 STCGRLLNDWRSFKRESEEGKLNAISLYMIHSGGASTEEETIEHFKGLIDSQRRQLLQLV 720 LQEKDSIIPRPCKDLFWNMIKLLHTFYMKDDGFTSNEMRNVVKAIINEPISLDEL 775 SEQIDNO:51 ArtificialSequence atgtctatcaaccttcgctcctccggttgttcgtctccgatctcagctactttggaacga 60 ggattggactcagaagtacagacaagagctaacaatgtgagctttgagcaaacaaaggag 120 aagattaggaagatgttggagaaagtggagctttctgtttcggcctacgatactagttgg 180 gtagcaatggttccatcaccgagctcccaaaatgctccacttttcccacagtgtgtgaaa 240 tggttattggataatcaacatgaagatggatcttggggacttgataaccatgaccatcaa 300 tctcttaagaaggatgtgttatcatctacactggctagtatcctcgcgttaaagaagtgg 360 ggaattggtgaaagacaaataaacaagggtctccagtttattgagctgaattctgcatta 420 gtcactgatgaaaccatacagaaaccaacagggtttgatattatatttcctgggatgatt 480 aaatatgctagagatttgaatctgacgattccattgggctcagaagtggtggatgacatg 540 atacgaaaaagagatctggatcttaaatgtgatagtgaaaagttttcaaagggaagagaa 600 gcatatctggcctatgttttagaggggacaagaaacctaaaagattgggatttgatagtc 660 aaatatcaaaggaaaaatgggtcactgtttgattctccagccacaacagcagctgctttt 720 actcagtttgggaatgatggttgtctccgttatctctgttctctccttcagaaattcgag 780 gctgcagttccttcagtttatccatttgatcaatatgcacgccttagtataattgtcact 840 cttgaaagcttaggaattgatagagatttcaaaaccgaaatcaaaagcatattggatgaa 900 acctatagatattggcttcgtggggatgaagaaatatgtttggacttggccacttgtgct 960 ttggctttccgattattgcttgctcatggctatgatgtgtcttacgatccgctaaaacca 1020 tttgcagaagaatctggtttctctgatactttggaaggatatgttaagaatacgttttct 1080 gtgttagaattatttaaggctgctcaaagttatccacatgaatcagctttgaagaagcag 1140 tgttgttggactaaacaatatctggagatggaattgtccagctgggttaagacctctgtt 1200 cgagataaatacctcaagaaagaggtcgaggatgctcttgcttttccctcctatgcaagc 1260 ctagaaagatcagatcacaggagaaaaatactcaatggttctgctgtggaaaacaccaga 1320 gttacaaaaacctcatatcgtttgcacaatatttgcacctctgatatcctgaagttagct 1380 gtggatgacttcaatttctgccagtccatacaccgtgaagaaatggaacgtcttgatagg 1440 tggattgtggagaatagattgcaggaactgaaatttgccagacagaagctggcttactgt 1500 tatttctctggggctgcaactttattttctccagaactatctgatgctcgtatatcgtgg 1560 gccaaaggtggagtacttacaacggttgtagacgacttctttgatgttggagggtccaaa 1620 gaagaactggaaaacctcatacacttggtcgaaaagtgggatttgaacggtgttcctgag 1680 tacagctcagaacatgttgagatcatattctcagttctaagggacaccattctcgaaaca 1740 ggagacaaagcattcacctatcaaggacgcaatgtgacacaccacattgtgaaaatttgg 1800 ttggatctgctcaagtctatgttgagagaagccgagtggtccagtgacaagtcaacacca 1860 agcttggaggattacatggaaaatgcgtacatatcatttgcattaggaccaattgtcctc 1920 ccagctacctatctgatcggacctccacttccagagaagacagtcgatagccaccaatat 1980 aatcagctctacaagctcgtgagcactatgggtcgtcttctaaatgacatacaaggtttt 2040 aagagagaaagcgcggaagggaagctgaatgcggtttcattgcacatgaaacacgagaga 2100 gacaatcgcagcaaagaagtgatcatagaatcgatgaaaggtttagcagagagaaagagg 2160 gaagaattgcataagctagttttggaggagaaaggaagtgtggttccaagggaatgcaaa 2220 gaagcgttcttgaaaatgagcaaagtgttgaacttattttacaggaaggacgatggattc 2280 acatcaaatgatctgatgagtcttgttaaatcagtgatctacgagcctgttagcttacag 2340 aaagaatctttaacttga 2358 SEQIDNO:52 Arabidopsisthaliana MSINLRSSGCSSPISATLERGLDSEVQTRANNVSFEQTKEKIRKMLEKVELSVSAYDTSW 60 VAMVPSPSSQNAPLFPQCVKWLLDNQHEDGSWGLDNHDHQSLKKDVLSSTLASILALKKW 120 GIGERQINKGLQFIELNSALVTDETIQKPTGFDIIFPGMIKYARDLNLTIPLGSEVVDDM 180 IRKRDLDLKCDSEKFSKGREAYLAYVLEGTRNLKDWDLIVKYQRKNGSLFDSPATTAAAF 240 TQFGNDGCLRYLCSLLQKFEAAVPSVYPFDQYARLSIIVTLESLGIDRDFKTEIKSILDE 300 TYRYWLRGDEEICLDLATCALAFRLLLAHGYDVSYDPLKPFAEESGFSDTLEGYVKNTFS 360 VLELFKAAQSYPHESALKKQCCWTKQYLEMELSSWVKTSVRDKYLKKEVEDALAFPSYAS 420 LERSDHRRKILNGSAVENTRVTKTSYRLHNICTSDILKLAVDDFNFCQSIHREEMERLDR 480 WIVENRLQELKFARQKLAYCYFSGAATLFSPELSDARISWAKGGVLTTVVDDFFDVGGSK 540 EELENLIHLVEKWDLNGVPEYSSEHVEIIFSVLRDTILETGDKAFTYQGRNVTHHIVKIW 600 LDLLKSMLREAEWSSDKSTPSLEDYMENAYISFALGPIVLPATYLIGPPLPEKTVDSHQY 660 NQLYKLVSTMGRLLNDIQGFKRESAEGKLNAVSLHMKHERDNRSKEVIIESMKGLAERKR 720 EELHKLVLEEKGSVVPRECKEAFLKMSKVLNLFYRKDDGFTSNDLMSLVKSVIYEPVSLQ 780 KESLT 785 SEQIDNO:53 ArtificialSequence atggaatttgatgaaccattggttgacgaagcaagatctttagtgcagcgtactttacaa 60 gattatgatgacagatacggcttcggtactatgtcatgtgctgcttatgatacagcctgg 120 gtgtctttagttacaaaaacagtcgatgggagaaaacaatggcttttcccagagtgtttt 180 gaatttctactagaaacacaatctgatgccggaggatgggaaatcgggaattcagcacca 240 atcgacggtatattgaatacagctgcatccttacttgctctaaaacgtcacgttcaaact 300 gagcaaatcatccaacctcaacatgaccataaggatctagcaggtagagctgaacgtgcc 360 gctgcatctttgagagcacaattggctgcattggatgtgtctacaactgaacacgtcggt 420 tttgagataattgttcctgcaatgctagacccattagaagccgaagatccatctctagtt 480 ttcgattttccagctaggaaacctttgatgaagattcatgatgctaagatgagtagattc 540 aggccagaatacttgtatggcaaacaaccaatgaccgccttacattcattagaggctttc 600 ataggcaaaatcgacttcgataaggtaagacaccaccgtacccatgggtctatgatgggt 660 tctccttcatctaccgcagcctacttaatgcacgcttcacaatgggatggtgactcagag 720 gcttaccttagacacgtgattaaacacgcagcagggcagggaactggtgctgtaccatct 780 gctttcccatcaacacattttgagtcatcttggattcttaccacattgtttagagctgga 840 ttttcagcttctcatcttgcctgtgatgagttgaacaagttggtcgagatacttgagggc 900 tcattcgagaaggaaggtggggcaatcggttacgctccagggtttcaagcagatgttgat 960 gatactgctaaaacaataagtacattagcagtccttggaagagatgctacaccaagacaa 1020 atgatcaaggtatttgaagctaatacacattttagaacataccctggtgaaagagatcct 1080 tctttgacagctaattgtaatgctctatcagccttactacaccaaccagatgcagcaatg 1140 tatggatctcaaattcaaaagattaccaaatttgtctgtgactattggtggaagtctgat 1200 ggtaagattaaagataagtggaacacttgctacttgtacccatctgtcttattagttgag 1260 gttttggttgatcttgttagtttattggagcagggtaaattgcctgatgttttggatcaa 1320 gagcttcaatacagagtcgccatcacattgttccaagcatgtttaaggccattactagac 1380 caagatgccgaaggatcatggaacaagtctatcgaagccacagcctacggcatccttatc 1440 ctaactgaagctaggagagtttgtttcttcgacagattgtctgagccattgaatgaggca 1500 atccgtagaggtatcgctttcgccgactctatgtctggaactgaagctcagttgaactac 1560 atttggatcgaaaaggttagttacgcacctgcattattgactaaatcctatttgttagca 1620 gcaagatgggctgctaagtctcctttaggcgcttccgtaggctcttctttgtggactcca 1680 ccaagagaaggattggataagcatgtcagattattccatcaagctgagttattcagatcc 1740 cttccagaatgggaattaagagcctccatgattgaagcagctttgttcacaccacttcta 1800 agagcacatagactagacgttttccctagacaagatgtaggtgaagacaaatatcttgat 1860 gtagttccattcttttggactgccgctaacaacagagatagaacttacgcttccactcta 1920 ttcctttacgatatgtgttttatcgcaatgttaaacttccagttagacgaattcatggag 1980 gccacagccggtatcttattcagagatcatatggatgatttgaggcaattgattcatgat 2040 cttttggcagagaaaacttccccaaagagttctggtagaagtagtcagggcacaaaagat 2100 gctgactcaggtatagaggaagacgtgtcaatgtccgattcagcttcagattcccaggat 2160 agaagtccagaatacgacttggttttcagtgcattgagtacctttacaaaacatgtcttg 2220 caacacccatctatacaaagtgcctctgtatgggatagaaaactacttgctagagagatg 2280 aaggcttacttacttgctcatatccaacaagcagaagattcaactccattgtctgaattg 2340 aaagatgtgcctcaaaagactgatgtaacaagagtttctacatctactactaccttcttt 2400 aactgggttagaacaacttccgcagaccatatatcctgcccatactccttccactttgta 2460 gcatgccatctaggcgcagcattgtcacctaaagggtctaacggtgattgctatccttca 2520 gctggtgagaagttcttggcagctgcagtctgcagacatttggccaccatgtgtagaatg 2580 tacaacgatcttggatcagctgaacgtgattctgatgaaggtaatttgaactccttggac 2640 ttccctgaattcgccgattccgcaggaaacggagggatagaaattcagaaggccgctcta 2700 ttaaggttagctgagtttgagagagattcatacttagaggccttccgtcgtttacaagat 2760 gaatccaatagagttcacggtccagccggtggtgatgaagccagattgtccagaaggaga 2820 atggcaatccttgaattcttcgcccagcaggtagatttgtacggtcaagtatacgtcatt 2880 agggatatttccgctcgtattcctaaaaacgaggttgagaaaaagagaaaattggatgat 2940 gctttcaattga 2952 SEQIDNO:54 Phomopsisamygdali MEFDEPLVDEARSLVQRTLQDYDDRYGFGTMSCAAYDTAWVSLVTKTVDGRKQWLFPECF 60 EFLLETQSDAGGWEIGNSAPIDGILNTAASLLALKRHVQTEQIIQPQHDHKDLAGRAERA 120 AASLRAQLAALDVSTTEHVGFEIIVPAMLDPLEAEDPSLVFDFPARKPLMKIHDAKMSRF 180 RPEYLYGKQPMTALHSLEAFIGKIDFDKVRHHRTHGSMMGSPSSTAAYLMHASQWDGDSE 240 AYLRHVIKHAAGQGTGAVPSAFPSTHFESSWILTTLFRAGFSASHLACDELNKLVEILEG 300 SFEKEGGAIGYAPGFQADVDDTAKTISTLAVLGRDATPRQMIKVFEANTHFRTYPGERDP 360 SLTANCNALSALLHQPDAAMYGSQIQKITKFVCDYWWKSDGKIKDKWNTCYLYPSVLLVE 420 VLVDLVSLLEQGKLPDVLDQELQYRVAITLFQACLRPLLDQDAEGSWNKSIEATAYGILI 480 LTEARRVCFFDRLSEPLNEAIRRGIAFADSMSGTEAQLNYIWIEKVSYAPALLTKSYLLA 540 ARWAAKSPLGASVGSSLWTPPREGLDKHVRLFHQAELFRSLPEWELRASMIEAALFTPLL 600 RAHRLDVFPRQDVGEDKYLDVVPFFWTAANNRDRTYASTLFLYDMCFIAMLNFQLDEFME 660 ATAGILFRDHMDDLRQLIHDLLAEKTSPKSSGRSSQGTKDADSGIEEDVSMSDSASDSQD 720 RSPEYDLVFSALSTFTKHVLQHPSIQSASVWDRKLLAREMKAYLLAHIQQAEDSTPLSEL 780 KDVPQKTDVTRVSTSTTTFFNWVRTTSADHISCPYSFHFVACHLGAALSPKGSNGDCYPS 840 AGEKFLAAAVCRHLATMCRMYNDLGSAERDSDEGNLNSLDFPEFADSAGNGGIEIQKAAL 900 LRLAEFERDSYLEAFRRLQDESNRVHGPAGGDEARLSRRRMAILEFFAQQVDLYGQVYVI 960 RDISARIPKNEVEKKRKLDDAFN 983 SEQIDNO:55 ArtificialSequence atggcttctagtacacttatccaaaacagatcatgtggcgtcacatcatctatgtcaagt 60 tttcaaatcttcagaggtcaaccactaagatttcctggcactagaaccccagctgcagtt 120 caatgcttgaaaaagaggagatgccttaggccaaccgaatccgtactagaatcatctcct 180 ggctctggttcatatagaatagtaactggcccttctggaattaaccctagttctaacggg 240 cacttgcaagagggttccttgactcacaggttaccaataccaatggaaaaatctatcgat 300 aacttccaatctactctatatgtgtcagatatttggtctgaaacactacagagaactgaa 360 tgtttgctacaagtaactgaaaacgtccagatgaatgagtggattgaggaaattagaatg 420 tactttagaaatatgactttaggtgaaatttccatgtccccttacgacactgcttgggtg 480 gctagagttccagcgttggacggttctcatgggcctcaattccacagatctttgcaatgg 540 attatcgacaaccaattaccagatggggactggggcgaaccttctcttttcttgggttac 600 gatagagtttgtaatactttagcctgtgtgattgcgttgaaaacatggggtgttggggca 660 caaaacgttgaaagaggaattcagttcctacaatctaacatatacaagatggaggaagat 720 gacgctaatcatatgccaataggattcgaaatcgtattccctgctatgatggaagatgcc 780 aaagcattaggtttggatttgccatacgatgctactattttgcaacagatttcagccgaa 840 agagagaaaaagatgaaaaagatcccaatggcaatggtgtacaaatacccaaccacttta 900 cttcactccttagaaggcttgcatagagaagttgattggaataagttgttacaattacaa 960 tctgaaaatggtagttttctttattcacctgcttcaaccgcatgcgccttaatgtacact 1020 aaggacgttaaatgttttgattacttaaaccagttgttgatcaagttcgaccacgcatgc 1080 ccaaatgtatatccagtcgatctattcgaaagattatggatggttgacagattgcagaga 1140 ttagggatctccagatactttgaaagagagattagagattgtttacaatacgtctacaga 1200 tattggaaagattgtggaatcggatgggcttctaactcttccgtacaagatgttgatgat 1260 acagccatggcgtttagacttttaaggactcatggtttcgacgtaaaggaagattgcttt 1320 agacagtttttcaaggacggagaattcttctgcttcgcaggccaatcatctcaagcagtt 1380 acaggcatgtttaatctttcaagagccagtcaaacattgtttccaggagaatctttattg 1440 aaaaaggctagaaccttctctagaaacttcttgagaacaaagcatgagaacaacgaatgt 1500 ttcgataaatggatcattactaaagatttggctggtgaagtcgagtataacttgaccttc 1560 ccatggtatgcctctttgcctagattagaacataggacatacttagatcaatatggaatc 1620 gatgatatctggataggcaaatctttatacaaaatgcctgctgttaccaacgaagttttc 1680 ctaaagttggcaaaggcagactttaacatgtgtcaagctctacacaaaaaggaattggaa 1740 caagtgataaagtggaacgcgtcctgtcaattcagagatcttgaattcgccagacaaaaa 1800 tcagtagaatgctattttgctggtgcagccacaatgttcgaaccagaaatggttcaagct 1860 agattagtctgggcaagatgttgtgtattgacaactgtcttagacgattactttgaccac 1920 gggacacctgttgaggaacttagagtgtttgttcaagctgtcagaacatggaatccagag 1980 ttgatcaacggtttgccagagcaagctaaaatcttgtttatgggcttatacaaaacagtt 2040 aacacaattgcagaggaagcattcatggcacagaaaagagacgtccatcatcatttgaaa 2100 cactattgggacaagttgataacaagtgccctaaaggaggccgaatgggcagagtcaggt 2160 tacgtcccaacatttgatgaatacatggaagtagctgaaatttctgttgctctagaacca 2220 attgtctgtagtaccttgttctttgcgggtcatagactagatgaggatgttctagatagt 2280 tacgattaccatctagttatgcatttggtaaacagagtcggtagaatcttgaatgatata 2340 caaggcatgaagagggaggcttcacaaggtaagatctcatcagttcaaatctacatggag 2400 gaacatccatctgttccatctgaggccatggcgatcgctcatcttcaagagttagttgat 2460 aattcaatgcagcaattgacatacgaagttcttaggttcactgcggttccaaaaagttgt 2520 aagagaatccacttgaatatggctaaaatcatgcatgccttctacaaggatactgatgga 2580 ttctcatcccttactgcaatgacaggattcgtcaaaaaggttcttttcgaacctgtgcct 2640 gagtaa 2646 SEQIDNO:56 Physcomitrellapatens MASSTLIQNRSCGVTSSMSSFQIFRGQPLRFPGTRTPAAVQCLKKRRCLRPTESVLESSP 60 GSGSYRIVTGPSGINPSSNGHLQEGSLTHRLPIPMEKSIDNFQSTLYVSDIWSETLQRTE 120 CLLQVTENVQMNEWIEEIRMYFRNMTLGEISMSPYDTAWVARVPALDGSHGPQFHRSLQW 180 IIDNQLPDGDWGEPSLFLGYDRVCNTLACVIALKTWGVGAQNVERGIQFLQSNIYKMEED 240 DANHMPIGFEIVFPAMMEDAKALGLDLPYDATILQQISAEREKKMKKIPMAMVYKYPTTL 300 LHSLEGLHREVDWNKLLQLQSENGSFLYSPASTACALMYTKDVKCFDYLNQLLIKFDHAC 360 PNVYPVDLFERLWMVDRLQRLGISRYFEREIRDCLQYVYRYWKDCGIGWASNSSVQDVDD 420 TAMAFRLLRTHGFDVKEDCFRQFFKDGEFFCFAGQSSQAVTGMFNLSRASQTLFPGESLL 480 KKARTFSRNFLRTKHENNECFDKWIITKDLAGEVEYNLTFPWYASLPRLEHRTYLDQYGI 540 DDIWIGKSLYKMPAVTNEVFLKLAKADFNMCQALHKKELEQVIKWNASCQFRDLEFARQK 600 SVECYFAGAATMFEPEMVQARLVWARCCVLTTVLDDYFDHGTPVEELRVFVQAVRTWNPE 660 LINGLPEQAKILFMGLYKTVNTIAEEAFMAQKRDVHHHLKHYWDKLITSALKEAEWAESG 720 YVPTFDEYMEVAEISVALEPIVCSTLFFAGHRLDEDVLDSYDYHLVMHLVNRVGRILNDI 780 QGMKREASQGKISSVQIYMEEHPSVPSEAMAIAHLQELVDNSMQQLTYEVLRFTAVPKSC 840 KRIHLNMAKIMHAFYKDTDGFSSLTAMTGFVKKVLFEPVPE 881 SEQIDNO:57 ArtificialSequence atgcctggtaaaattgaaaatggtaccccaaaggacctcaagactggaaatgattttgtt 60 tctgctgctaagagtttactagatcgagctttcaaaagtcatcattcctactacggatta 120 tgctcaacttcatgtcaagtttatgatacagcttgggttgcaatgattccaaaaacaaga 180 gataatgtaaaacagtggttgtttccagaatgtttccattacctcttaaaaacacaagcc 240 gcagatggctcatggggttcattgcctacaacacagacagcgggtatcctagatacagcc 300 tcagctgtgctggcattattgtgccacgcacaagagcctttacaaatattggatgtatct 360 ccagatgaaatggggttgagaatagaacacggtgtcacatccttgaaacgtcaattagca 420 gtttggaatgatgtggaggacaccaaccatattggcgtcgagtttatcataccagcctta 480 ctttccatgctagaaaaggaattagatgttccatcttttgaatttccatgtaggtccatc 540 ttagagagaatgcacggggagaaattaggtcatttcgacctggaacaagtttacggcaag 600 ccaagctcattgttgcactcattggaagcatttctcggtaagctagattttgatcgacta 660 tcacatcacctataccacggcagtatgatggcatctccatcttcaacggctgcttatctt 720 attggggctacaaaatgggatgacgaagccgaagattacctaagacatgtaatgcgtaat 780 ggtgcaggacatgggaatggaggtatttctggtacatttccaactactcatttcgaatgt 840 agctggattatagcaacgttgttaaaggttggctttactttgaagcaaattgacggcgat 900 ggcttaagaggtttatcaaccatcttacttgaggcgcttcgtgatgagaatggtgtcata 960 ggctttgcccctagaacagcagatgtagatgacacagccaaagctctattggccttgtca 1020 ttggtaaaccagccagtgtcacctgatatcatgattaaggtctttgagggcaaagaccat 1080 tttaccacttttggttcagaaagagatccatcattgacttccaacctgcacgtcctttta 1140 tctttacttaaacaatctaacttgtctcaataccatcctcaaatcctcaaaacaacatta 1200 ttcacttgtagatggtggtggggttccgatcattgtgtcaaagacaaatggaatttgagt 1260 cacctatatccaactatgttgttggttgaagccttcactgaagtgctccatctcattgac 1320 ggtggtgaattgtctagtctgtttgatgaatcctttaagtgtaagattggtcttagcatc 1380 tttcaagcggtacttagaataatcctcacccaagacaacgacggctcttggagaggatac 1440 agagaacagacgtgttacgcaatattggctttagttcaagcgagacatgtatgctttttc 1500 actcacatggttgacagactgcaatcatgtgttgatcgaggtttctcatggttgaaatct 1560 tgctcttttcattctcaagacctgacttggacctctaaaacagcttatgaagtgggtttc 1620 gtagctgaagcatataaactagctgctttacaatctgcttccctggaggttcctgctgcc 1680 accattggacattctgtcacgtctgccgttccatcaagtgatcttgaaaaatacatgaga 1740 ttggtgagaaaaactgcgttattctctccactggatgagtggggtctaatggcttctatc 1800 atcgaatcttcatttttcgtaccattactgcaggcacaaagagttgaaatataccctaga 1860 gataatatcaaggtggacgaagataagtacttgtctattatcccattcacatgggtcgga 1920 tgcaataataggtctagaactttcgcaagtaacagatggctatacgatatgatgtacctt 1980 tcattactcggctatcaaaccgacgagtacatggaagctgtagctgggccagtgtttggg 2040 gatgtttccttgttacatcaaacaattgataaggtgattgataatacaatgggtaacctt 2100 gcgagagccaatggaacagtacacagtggtaatggacatcagcacgaatctcctaatata 2160 ggtcaagtcgaggacaccttgactcgtttcacaaattcagtcttgaatcacaaagacgtc 2220 cttaactctagctcatctgatcaagatactttgagaagagagtttagaacattcatgcac 2280 gctcatataacacaaatcgaagataactcacgattcagtaagcaagcctcatccgatgcg 2340 ttttcctctcctgaacaatcttactttcaatgggtgaactcaactggtggctcacatgtc 2400 gcttgcgcctattcatttgccttctctaattgcctcatgtctgcaaatttgttgcagggt 2460 aaagacgcatttccaagcggaacgcaaaagtacttaatctcctctgttatgagacatgcc 2520 acaaacatgtgtagaatgtataacgactttggctctattgccagagacaacgctgagaga 2580 aatgttaatagtattcattttcctgagtttactctctgtaacggaacttctcaaaaccta 2640 gatgaaaggaaggaaagacttctgaaaatcgcaacttacgaacaagggtatttggataga 2700 gcactagaggccttggaaagacagagtagagatgatgccggagacagagctggatctaaa 2760 gatatgagaaagttgaaaatcgttaagttattctgtgatgttacggacttatacgatcag 2820 ctctacgttatcaaagatttgtcatcctctatgaagtaa 2859 SEQIDNO:58 Gibberellafujikuroi MPGKIENGTPKDLKTGNDFVSAAKSLLDRAFKSHHSYYGLCSTSCQVYDTAWVAMIPKTR 60 DNVKQWLFPECFHYLLKTQAADGSWGSLPTTQTAGILDTASAVLALLCHAQEPLQILDVS 120 PDEMGLRIEHGVTSLKRQLAVWNDVEDTNHIGVEFIIPALLSMLEKELDVPSFEFPCRSI 180 LERMHGEKLGHFDLEQVYGKPSSLLHSLEAFLGKLDFDRLSHHLYHGSMMASPSSTAAYL 240 IGATKWDDEAEDYLRHVMRNGAGHGNGGISGTFPTTHFECSWIIATLLKVGFTLKQIDGD 300 GLRGLSTILLEALRDENGVIGFAPRTADVDDTAKALLALSLVNQPVSPDIMIKVFEGKDH 360 FTTFGSERDPSLTSNLHVLLSLLKQSNLSQYHPQILKTTLFTCRWWWGSDHCVKDKWNLS 420 HLYPTMLLVEAFTEVLHLIDGGELSSLFDESFKCKIGLSIFQAVLRIILTQDNDGSWRGY 480 REQTCYAILALVQARHVCFFTHMVDRLQSCVDRGFSWLKSCSFHSQDLTWTSKTAYEVGF 540 VAEAYKLAALQSASLEVPAATIGHSVTSAVPSSDLEKYMRLVRKTALFSPLDEWGLMASI 600 IESSFFVPLLQAQRVEIYPRDNIKVDEDKYLSIIPFTWVGCNNRSRTFASNRWLYDMMYL 660 SLLGYQTDEYMEAVAGPVFGDVSLLHQTIDKVIDNTMGNLARANGTVHSGNGHQHESPNI 720 GQVEDTLTRFTNSVLNHKDVLNSSSSDQDTLRREFRTFMHAHITQIEDNSRFSKQASSDA 780 FSSPEQSYFQWVNSTGGSHVACAYSFAFSNCLMSANLLQGKDAFPSGTQKYLISSVMRHA 840 TNMCRMYNDFGSIARDNAERNVNSIHFPEFTLCNGTSQNLDERKERLLKIATYEQGYLDR 900 ALEALERQSRDDAGDRAGSKDMRKLKIVKLFCDVTDLYDQLYVIKDLSSSMK 952 SEQIDNO:59 ArtificialSequence atggatgctgtgacgggtttgttaactgtcccagcaaccgctataactattggtggaact 60 gctgtagcattggcggtagcgctaatcttttggtacctgaaatcctacacatcagctaga 120 agatcccaatcaaatcatcttccaagagtgcctgaagtcccaggtgttccattgttagga 180 aatctgttacaattgaaggagaaaaagccatacatgacttttacgagatgggcagcgaca 240 tatggacctatctatagtatcaaaactggggctacaagtatggttgtggtatcatctaat 300 gagatagccaaggaggcattggtgaccagattccaatccatatctacaaggaacttatct 360 aaagccctgaaagtacttacagcagataagacaatggtcgcaatgtcagattatgatgat 420 tatcataaaacagttaagagacacatactgaccgccgtcttgggtcctaatgcacagaaa 480 aagcatagaattcacagagatatcatgatggataacatatctactcaacttcatgaattc 540 gtgaaaaacaacccagaacaggaagaggtagaccttagaaaaatctttcaatctgagtta 600 ttcggcttagctatgagacaagccttaggaaaggatgttgaaagtttgtacgttgaagac 660 ctgaaaatcactatgaatagagacgaaatctttcaagtccttgttgttgatccaatgatg 720 ggagcaatcgatgttgattggagagacttctttccatacctaaagtgggtcccaaacaaa 780 aagttcgaaaatactattcaacaaatgtacatcagaagagaagctgttatgaaatcttta 840 atcaaagagcacaaaaagagaatagcgtcaggcgaaaagctaaatagttatatcgattac 900 cttttatctgaagctcaaactttaaccgatcagcaactattgatgtccttgtgggaacca 960 atcattgaatcttcagatacaacaatggtcacaacagaatgggcaatgtacgaattagct 1020 aaaaaccctaaattgcaagataggttgtacagagacattaagtccgtctgtggatctgaa 1080 aagataaccgaagagcatctatcacagctgccttacattacagctattttccacgaaaca 1140 ctgagaagacactcaccagttcctatcattcctctaagacatgtacatgaagataccgtt 1200 ctaggcggctaccatgttcctgctggcacagaacttgccgttaacatctacggttgcaac 1260 atggacaaaaacgtttgggaaaatccagaggaatggaacccagaaagattcatgaaagag 1320 aatgagacaattgattttcaaaagacgatggccttcggtggtggtaagagagtttgtgct 1380 ggttccttgcaagcccttttaactgcatctattgggattgggagaatggttcaagagttc 1440 gaatggaaactgaaggatatgactcaagaggaagtgaacacgataggcctaactacacaa 1500 atgttaagaccattgagagctattatcaaacctaggatctaa 1542 SEQIDNO:60 Steviarebaudiana MDAVTGLLTVPATAITIGGTAVALAVALIFWYLKSYTSARRSQSNHLPRVPEVPGVPLLG 60 NLLQLKEKKPYMTFTRWAATYGPIYSIKTGATSMVVVSSNEIAKEALVTRFQSISTRNLS 120 KALKVLTADKTMVAMSDYDDYHKTVKRHILTAVLGPNAQKKHRIHRDIMMDNISTQLHEF 180 VKNNPEQEEVDLRKIFQSELFGLAMRQALGKDVESLYVEDLKITMNRDEIFQVLVVDPMM 240 GAIDVDWRDFFPYLKWVPNKKFENTIQQMYIRREAVMKSLIKEHKKRIASGEKLNSYIDY 300 LLSEAQTLTDQQLLMSLWEPIIESSDTTMVTTEWAMYELAKNPKLQDRLYRDIKSVCGSE 360 KITEEHLSQLPYITAIFHETLRRHSPVPIIPLRHVHEDTVLGGYHVPAGTELAVNIYGCN 420 MDKNVWENPEEWNPERFMKENETIDFQKTMAFGGGKRVCAGSLQALLTASIGIGRMVQEF 480 EWKLKDMTQEEVNTIGLTTQMLRPLRAIIKPRI 513 SEQIDNO:61 ArtificialSequence aagcttactagtaaaatggacggtgtcatcgatatgcaaaccattccattgagaaccgct 60 attgctattggtggtactgctgttgctttggttgttgcattatacttttggttcttgaga 120 tcctacgcttccccatctcatcattctaatcatttgccaccagtacctgaagttccaggt 180 gttccagttttgggtaatttgttgcaattgaaagaaaaaaagccttacatgaccttcacc 240 aagtgggctgaaatgtatggtccaatctactctattagaactggtgctacttccatggtt 300 gttgtctcttctaacgaaatcgccaaagaagttgttgttaccagattcccatctatctct 360 accagaaaattgtcttacgccttgaaggttttgaccgaagataagtctatggttgccatg 420 tctgattatcacgattaccataagaccgtcaagagacatattttgactgctgttttgggt 480 ccaaacgcccaaaaaaagtttagagcacatagagacaccatgatggaaaacgtttccaat 540 gaattgcatgccttcttcgaaaagaacccaaatcaagaagtcaacttgagaaagatcttc 600 caatcccaattattcggtttggctatgaagcaagccttgggtaaagatgttgaatccatc 660 tacgttaaggatttggaaaccaccatgaagagagaagaaatcttcgaagttttggttgtc 720 gatccaatgatgggtgctattgaagttgattggagagactttttcccatacttgaaatgg 780 gttccaaacaagtccttcgaaaacatcatccatagaatgtacactagaagagaagctgtt 840 atgaaggccttgatccaagaacacaagaaaagaattgcctccggtgaaaacttgaactcc 900 tacattgattacttgttgtctgaagcccaaaccttgaccgataagcaattattgatgtct 960 ttgtgggaacctattatcgaatcttctgataccactatggttactactgaatgggctatg 1020 tacgaattggctaagaatccaaacatgcaagacagattatacgaagaaatccaatccgtt 1080 tgcggttccgaaaagattactgaagaaaacttgtcccaattgccatacttgtacgctgtt 1140 ttccaagaaactttgagaaagcactgtccagttcctattatgccattgagatatgttcac 1200 gaaaacaccgttttgggtggttatcatgttccagctggtactgaagttgctattaacatc 1260 tacggttgcaacatggataagaaggtctgggaaaatccagaagaatggaatccagaaaga 1320 ttcttgtccgaaaaagaatccatggacttgtacaaaactatggcttttggtggtggtaaa 1380 agagtttgcgctggttctttacaagccatggttatttcttgcattggtatcggtagattg 1440 gtccaagattttgaatggaagttgaaggatgatgccgaagaagatgttaacactttgggt 1500 ttgactacccaaaagttgcatccattattggccttgattaacccaagaaagtaactcgag 1560 ccgcgg 1566 SEQIDNO:62 Lactucasativa MDGVIDMQTIPLRTAIAIGGTAVALVVALYFWFLRSYASPSHHSNHLPPVPEVPGVPVLG 60 NLLQLKEKKPYMTFTKWAEMYGPIYSIRTGATSMVVVSSNEIAKEVVVTRFPSISTRKLS 120 YALKVLTEDKSMVAMSDYHDYHKTVKRHILTAVLGPNAQKKFRAHRDTMMENVSNELHAF 180 FEKNPNQEVNLRKIFQSQLFGLAMKQALGKDVESIYVKDLETTMKREEIFEVLVVDPMMG 240 AIEVDWRDFFPYLKWVPNKSFENIIHRMYTRREAVMKALIQEHKKRIASGENLNSYIDYL 300 LSEAQTLTDKQLLMSLWEPIIESSDTTMVTTEWAMYELAKNPNMQDRLYEEIQSVCGSEK 360 ITEENLSQLPYLYAVFQETLRKHCPVPIMPLRYVHENTVLGGYHVPAGTEVAINIYGCNM 420 DKKVWENPEEWNPERFLSEKESMDLYKTMAFGGGKRVCAGSLQAMVISCIGIGRLVQDFE 480 WKLKDDAEEDVNTLGLTTQKLHPLLALINPRK 512 SEQIDNO:63 Rubussuavissimus atggccaccctccttgagcatttccaagctatgccctttgccatccctattgcactggct 60 gctctgtcttggctgttcctcttttacatcaaagtttcattcttttccaacaagagtgct 120 caggctaagctccctcctgtgccagtggttcctgggctgccggtgattgggaatttactg 180 caactcaaggagaagaaaccctaccagacttttacaaggtgggctgaggagtatggacca 240 atctattctatcaggactggtgcttccaccatggtcgttctcaataccacccaagttgca 300 aaagaggccatggtgaccagatatttatccatctcaaccagaaagctatcaaacgcacta 360 aagattcttactgctgataaatgtatggttgcaataagtgactacaacgattttcacaag 420 atgataaagcgatacatactctcaaatgttcttggacctagtgctcagaagcgtcaccgg 480 agcaacagagataccttgagagctaatgtctgcagccgattgcattctcaagtaaagaac 540 tctcctcgagaagctgtgaatttcagaagagtttttgagtgggaactctttggaattgca 600 ttgaagcaagcctttggaaaggacatagaaaagcccatttatgtggaggaacttggcact 660 acactgtcaagagatgagatctttaaggttctagtgcttgacataatggagggtgcaatt 720 gaggttgattggagagatttcttcccttacctgagatggattccgaatacgcgcatggaa 780 acaaaaattcagcgactctatttccgcaggaaagcagtgatgactgccctgatcaacgag 840 cagaagaagcgaattgcttcaggagaggaaatcaactgttatatcgacttcttgcttaag 900 gaagggaagacactgacaatggaccaaataagtatgttgctttgggagacggttattgaa 960 acagcagatactacaatggtaacgacagaatgggctatgtatgaagttgctaaagactca 1020 aagcgtcaggatcgtctctatcaggaaatccaaaaggtttgtggatcggagatggttaca 1080 gaggaatacttgtcccaactgccgtacctgaatgcagttttccatgaaacgctaaggaag 1140 cacagtccggctgcgttagttcctttaagatatgcacatgaagatacccaactaggaggt 1200 tactacattccagctggaactgagattgctataaacatatacgggtgtaacatggacaag 1260 catcaatgggaaagccctgaggaatggaaaccggagagatttttggacccgaaatttgat 1320 cctatggatttgtacaagaccatggcttttggggctggaaagagggtatgtgctggttct 1380 cttcaggcaatgttaatagcgtgcccgacgattggtaggctggtgcaggagtttgagtgg 1440 aagctgagagatggagaagaagaaaatgtagatactgttgggctcaccactcacaaacgc 1500 tatccaatgcatgcaatcctgaagccaagaagtta 1535 SEQIDNO:64 ArtificialSequence atggctaccttgttggaacattttcaagctatgccattcgctattccaattgctttggct 60 gctttgtcttggttgtttttgttctacatcaaggtttctttcttctccaacaaatccgct 120 caagctaaattgccaccagttccagttgttccaggtttgccagttattggtaatttgttg 180 caattgaaagaaaagaagccataccaaaccttcactagatgggctgaagaatatggtcca 240 atctactctattagaactggtgcttctactatggttgtcttgaacactactcaagttgcc 300 aaagaagctatggttaccagatacttgtctatctctaccagaaagttgtccaacgccttg 360 aaaattttgaccgctgataagtgcatggttgccatttctgattacaacgatttccacaag 420 atgatcaagagatatatcttgtctaacgttttgggtccatctgcccaaaaaagacataga 480 tctaacagagataccttgagagccaacgtttgttctagattgcattcccaagttaagaac 540 tctccaagagaagctgtcaactttagaagagttttcgaatgggaattattcggtatcgct 600 ttgaaacaagccttcggtaaggatattgaaaagccaatctacgtcgaagaattgggtact 660 actttgtccagagatgaaatcttcaaggttttggtcttggacattatggaaggtgccatt 720 gaagttgattggagagattttttcccatacttgcgttggattccaaacaccagaatggaa 780 actaagatccaaagattatactttagaagaaaggccgttatgaccgccttgattaacgaa 840 caaaagaaaagaattgcctccggtgaagaaatcaactgctacatcgatttcttgttgaaa 900 gaaggtaagaccttgaccatggaccaaatctctatgttgttgtgggaaaccgttattgaa 960 actgctgataccacaatggttactactgaatgggctatgtacgaagttgctaaggattct 1020 aaaagacaagacagattataccaagaaatccaaaaggtctgcggttctgaaatggttaca 1080 gaagaatacttgtcccaattgccatacttgaatgctgttttccacgaaactttgagaaaa 1140 cattctccagctgctttggttccattgagatatgctcatgaagatactcaattgggtggt 1200 tattacattccagccggtactgaaattgccattaacatctacggttgcaacatggacaaa 1260 caccaatgggaatctccagaagaatggaagccagaaagatttttggatcctaagtttgac 1320 ccaatggacttgtacaaaactatggcttttggtgctggtaaaagagtttgcgctggttct 1380 ttacaagctatgttgattgcttgtccaaccatcggtagattggttcaagaatttgaatgg 1440 aagttgagagatggtgaagaagaaaacgttgatactgttggtttgaccacccataagaga 1500 tatccaatgcatgctattttgaagccaagatcttaa 1536 SEQIDNO:65 ArtificialSequence aagcttactagtaaaatggcctccatcacccatttcttacaagattttcaagctactcca 60 ttcgctactgcttttgctgttggtggtgtttctttgttgatattcttcttcttcatccgt 120 ggtttccactctactaagaaaaacgaatattacaagttgccaccagttccagttgttcca 180 ggtttgccagttgttggtaatttgttgcaattgaaagaaaagaagccatacaagactttc 240 ttgagatgggctgaaattcatggtccaatctactctattagaactggtgcttctaccatg 300 gttgttgttaactctactcatgttgccaaagaagctatggttaccagattctcttcaatc 360 tctaccagaaagttgtccaaggctttggaattattgacctccaacaaatctatggttgcc 420 acctctgattacaacgaatttcacaagatggtcaagaagtacatcttggccgaattattg 480 ggtgctaatgctcaaaagagacacagaattcatagagacaccttgatcgaaaacgtcttg 540 aacaaattgcatgcccataccaagaattctccattgcaagctgttaacttcagaaagatc 600 ttcgaatctgaattattcggtttggctatgaagcaagccttgggttatgatgttgattcc 660 ttgttcgttgaagaattgggtactaccttgtccagagaagaaatctacaacgttttggtc 720 agtgacatgttgaagggtgctattgaagttgattggagagactttttcccatacttgaaa 780 tggatcccaaacaagtccttcgaaatgaagattcaaagattggcctctagaagacaagcc 840 gttatgaactctattgtcaaagaacaaaagaagtccattgcctctggtaagggtgaaaac 900 tgttacttgaattacttgttgtccgaagctaagactttgaccgaaaagcaaatttccatt 960 ttggcctgggaaaccattattgaaactgctgatacaactgttgttaccactgaatgggct 1020 atgtacgaattggctaaaaacccaaagcaacaagacagattatacaacgaaatccaaaac 1080 gtctgcggtactgataagattaccgaagaacatttgtccaagttgccttacttgtctgct 1140 gtttttcacgaaaccttgagaaagtattctccatctccattggttccattgagatacgct 1200 catgaagatactcaattgggtggttattatgttccagccggtactgaaattgctgttaat 1260 atctacggttgcaacatggacaagaatcaatgggaaactccagaagaatggaagccagaa 1320 agatttttggacgaaaagtacgatccaatggacatgtacaagactatgtcttttggttcc 1380 ggtaaaagagtttgcgctggttctttacaagctagtttgattgcttgtacctccatcggt 1440 agattggttcaagaatttgaatggagattgaaagacggtgaagttgaaaacgttgatacc 1500 ttgggtttgactacccataagttgtatccaatgcaagctatcttgcaacctagaaactga 1560 ctcgagccgcgg 1572 SEQIDNO:66 Castaneamollissima MASITHFLQDFQATPFATAFAVGGVSLLIFFFFIRGFHSTKKNEYYKLPPVPVVPGLPVV 60 GNLLQLKEKKPYKTFLRWAEIHGPIYSIRTGASTMVVVNSTHVAKEAMVTRFSSISTRKL 120 SKALELLTSNKSMVATSDYNEFHKMVKKYILAELLGANAQKRHRIHRDTLIENVLNKLHA 180 HTKNSPLQAVNFRKIFESELFGLAMKQALGYDVDSLFVEELGTTLSREEIYNVLVSDMLK 240 GAIEVDWRDFFPYLKWIPNKSFEMKIQRLASRRQAVMNSIVKEQKKSIASGKGENCYLNY 300 LLSEAKTLTEKQISILAWETIIETADTTVVTTEWAMYELAKNPKQQDRLYNEIQNVCGTD 360 KITEEHLSKLPYLSAVFHETLRKYSPSPLVPLRYAHEDTQLGGYYVPAGTEIAVNIYGCN 420 MDKNQWETPEEWKPERFLDEKYDPMDMYKTMSFGSGKRVCAGSLQASLIACTSIGRLVQE 480 FEWRLKDGEVENVDTLGLTTHKLYPMQAILQPRN 514 SEQIDNO:67 ArtificialSequence atgatttccttgttgttgggttttgttgtctcctccttcttgtttatcttcttcttgaaa 60 aaattgttgttcttcttcagtcgtcacaaaatgtccgaagtttctagattgccatctgtt 120 ccagttccaggttttccattgattggtaacttgttgcaattgaaagaaaagaagccacac 180 aagactttcaccaagtggtctgaattatatggtccaatctactctatcaagatgggttcc 240 tcttctttgatcgtcttgaactctattgaaaccgccaaagaagctatggtcagtagattc 300 tcttcaatctctaccagaaagttgtctaacgctttgactgttttgacctgcaacaaatct 360 atggttgctacctctgattacgatgactttcataagttcgtcaagagatgcttgttgaac 420 ggtttgttgggtgctaatgctcaagaaagaaaaagacattacagagatgccttgatcgaa 480 aacgttacctctaaattgcatgcccataccagaaatcatccacaagaaccagttaacttc 540 agagccattttcgaacacgaattattcggtgttgctttgaaacaagccttcggtaaagat 600 gtcgaatccatctatgtaaaagaattgggtgtcaccttgtccagagatgaaattttcaag 660 gttttggtccacgacatgatggaaggtgctattgatgttgattggagagatttcttccca 720 tacttgaaatggatcccaaacaactctttcgaagccagaattcaacaaaagcacaagaga 780 agattggctgttatgaacgccttgatccaagacagattgaatcaaaacgattccgaatcc 840 gatgatgactgctacttgaatttcttgatgtctgaagctaagaccttgaccatggaacaa 900 attgctattttggtttgggaaaccattatcgaaactgctgataccactttggttactact 960 gaatgggctatgtacgaattggccaaacatcaatctgttcaagatagattattcaaagaa 1020 atccaatccgtctgcggtggtgaaaagatcaaagaagaacaattgccaagattgccttac 1080 gtcaatggtgtttttcacgaaaccttgagaaagtattctccagctccattggttccaatt 1140 agatacgctcatgaagatacccaaattggtggttatcatattccagccggttctgaaatt 1200 gccattaacatctacggttgcaacatggataagaagagatgggaaagacctgaagaatgg 1260 tggccagaaagatttttggaagatagatacgaatcctccgacttgcataagactatggct 1320 tttggtgctggtaaaagagtttgtgctggtgctttacaagctagtttgatggctggtatt 1380 gctatcggtagattggttcaagaattcgaatggaagttgagagatggtgaagaagaaaac 1440 gttgatacttacggtttgacctcccaaaagttgtatccattgatggccattatcaaccca 1500 agaagatcttaa 1512 SEQIDNO:68 Thellungiellahalophila MASMISLLLGFVVSSFLFIFFLKKLLFFFSRHKMSEVSRLPSVPVPGFPLIGNLLQLKEK 60 KPHKTFTKWSELYGPIYSIKMGSSSLIVLNSIETAKEAMVSRFSSISTRKLSNALTVLTC 120 NKSMVATSDYDDFHKFVKRCLLNGLLGANAQERKRHYRDALIENVTSKLHAHTRNHPQEP 180 VNFRAIFEHELFGVALKQAFGKDVESIYVKELGVTLSRDEIFKVLVHDMMEGAIDVDWRD 240 FFPYLKWIPNNSFEARIQQKHKRRLAVMNALIQDRLNQNDSESDDDCYLNFLMSEAKTLT 300 MEQIAILVWETIIETADTTLVTTEWAMYELAKHQSVQDRLFKEIQSVCGGEKIKEEQLPR 360 LPYVNGVFHETLRKYSPAPLVPIRYAHEDTQIGGYHIPAGSEIAINIYGCNMDKKRWERP 420 EEWWPERFLEDRYESSDLHKTMAFGAGKRVCAGALQASLMAGIAIGRLVQEFEWKLRDGE 480 EENVDTYGLTSQKLYPLMAIINPRRS 506 SEQIDNO:69 ArtificialSequence aagcttactagtaaaatggacatgatgggtattgaagctgttccatttgctactgctgtt 60 gttttgggtggtatttccttggttgttttgatcttcatcagaagattcgtttccaacaga 120 aagagatccgttgaaggtttgccaccagttccagatattccaggtttaccattgattggt 180 aacttgttgcaattgaaagaaaagaagccacataagacctttgctagatgggctgaaact 240 tacggtccaattttctctattagaactggtgcttctaccatgatcgtcttgaattcttct 300 gaagttgccaaagaagctatggtcactagattctcttcaatctctaccagaaagttgtcc 360 aacgccttgaagattttgaccttcgataagtgtatggttgccacctctgattacaacgat 420 tttcacaaaatggtcaagggtttcatcttgagaaacgttttaggtgctccagcccaaaaa 480 agacatagatgtcatagagataccttgatcgaaaacatctctaagtacttgcatgcccat 540 gttaagacttctccattggaaccagttgtcttgaagaagattttcgaatccgaaattttc 600 ggtttggctttgaaacaagccttgggtaaggatatcgaatccatctatgttgaagaattg 660 ggtactaccttgtccagagaagaaatttttgccgttttggttgttgatccaatggctggt 720 gctattgaagttgattggagagattttttcccatacttgtcctggattccaaacaagtct 780 atggaaatgaagatccaaagaatggattttagaagaggtgctttgatgaaggccttgatt 840 ggtgaacaaaagaaaagaatcggttccggtgaagaaaagaactcctacattgatttcttg 900 ttgtctgaagctaccactttgaccgaaaagcaaattgctatgttgatctgggaaaccatc 960 atcgaaatttccgatacaactttggttacctctgaatgggctatgtacgaattggctaaa 1020 gacccaaatagacaagaaatcttgtacagagaaatccacaaggtttgcggttctaacaag 1080 ttgactgaagaaaacttgtccaagttgccatacttgaactctgttttccacgaaaccttg 1140 agaaagtattctccagctccaatggttccagttagatatgctcatgaagatactcaattg 1200 ggtggttaccatattccagctggttctcaaattgccattaacatctacggttgcaacatg 1260 aacaaaaagcaatgggaaaatcctgaagaatggaagccagaaagattcttggacgaaaag 1320 tatgacttgatggacttgcataagactatggcttttggtggtggtaaaagagtttgtgct 1380 ggtgctttacaagcaatgttgattgcttgcacttccatcggtagattcgttcaagaattt 1440 gaatggaagttgatgggtggtgaagaagaaaacgttgatactgttgctttgacctcccaa 1500 aaattgcatccaatgcaagccattattaaggccagagaatgactcgagccgcgg 1554 SEQIDNO:70 Vitisvinifera MDMMGIEAVPFATAVVLGGISLVVLIFIRRFVSNRKRSVEGLPPVPDIPGLPLIGNLLQL 60 KEKKPHKTFARWAETYGPIFSIRTGASTMIVLNSSEVAKEAMVTRFSSISTRKLSNALKI 120 LTFDKCMVATSDYNDFHKMVKGFILRNVLGAPAQKRHRCHRDTLIENISKYLHAHVKTSP 180 LEPVVLKKIFESEIFGLALKQALGKDIESIYVEELGTTLSREEIFAVLVVDPMAGAIEVD 240 WRDFFPYLSWIPNKSMEMKIQRMDFRRGALMKALIGEQKKRIGSGEEKNSYIDFLLSEAT 300 TLTEKQIAMLIWETIIEISDTTLVTSEWAMYELAKDPNRQEILYREIHKVCGSNKLTEEN 360 LSKLPYLNSVFHETLRKYSPAPMVPVRYAHEDTQLGGYHIPAGSQIAINIYGCNMNKKQW 420 ENPEEWKPERFLDEKYDLMDLHKTMAFGGGKRVCAGALQAMLIACTSIGRFVQEFEWKLM 480 GGEEENVDTVALTSQKLHPMQAIIKARE 508 SEQIDNO:71 ArtificialSequence aagcttaaaatgagtaagtctaatagtatgaattctacatcacacgaaaccctttttcaa 60 caattggtcttgggtttggaccgtatgccattgatggatgttcactggttgatctacgtt 120 gctttcggcgcatggttatgttcttatgtgatacatgttttatcatcttcctctacagta 180 aaagtgccagttgttggatacaggtctgtattcgaacctacatggttgcttagacttaga 240 ttcgtctgggaaggtggctctatcataggtcaagggtacaataagtttaaagactctatt 300 ttccaagttaggaaattgggaactgatattgtcattataccacctaactatattgatgaa 360 gtgagaaaattgtcacaggacaagactagatcagttgaacctttcattaatgattttgca 420 ggtcaatacacaagaggcatggttttcttgcaatctgacttacaaaaccgtgttatacaa 480 caaagactaactccaaaattggtttccttgaccaaggtcatgaaggaagagttggattat 540 gctttaacaaaagagatgcctgatatgaaaaatgacgaatgggtagaagtagatatcagt 600 agtataatggtgagattgatttccaggatctccgccagagtctttctagggcctgaacac 660 tgtcgtaaccaggaatggttgactactacagcagaatattcagaatcacttttcattaca 720 gggtttatcttaagagttgtacctcatatcttaagaccattcatcgcccctctattacct 780 tcatacaggactctacttagaaacgtttcaagtggtagaagagtcatcggtgacatcata 840 agatctcagcaaggggatggtaacgaagatatactttcctggatgagagatgctgccaca 900 ggagaggaaaagcaaatcgataacattgctcagagaatgttaattctttctttagcatca 960 atccacactactgcgatgaccatgacacatgccatgtacgatctatgtgcttgccctgag 1020 tacattgaaccattaagagatgaagttaaatctgttgttggggcttctggctgggacaag 1080 acagcgttaaacagatttcataagttggactccttcctaaaagagtcacaaagattcaac 1140 ccagtattcttattgacattcaatagaatctaccatcaatctatgaccttatcagatggc 1200 actaacattccatctggaacacgtattgctgttccatcacacgcaatgttgcaagattct 1260 gcacatgtcccaggtccaaccccacctactgaatttgatggattcagatatagtaagata 1320 cgttctgatagtaactacgcacaaaagtacctattctccatgaccgattcttcaaacatg 1380 gctttcggatacggcaagtatgcttgtccaggtagattttacgcgtctaatgagatgaaa 1440 ctaacattagccattttgttgctacaatttgagttcaaactaccagatggtaaaggtcgt 1500 cctagaaatatcactatcgattctgatatgattccagacccaagagctagactttgcgtc 1560 agaaaaagatcacttagagatgaatgaccgcgg 1593 SEQIDNO:72 Gibberellafujikuroi MSKSNSMNSTSHETLFQQLVLGLDRMPLMDVHWLIYVAFGAWLCSYVIHVLSSSSTVKVP 60 VVGYRSVFEPTWLLRLRFVWEGGSIIGQGYNKFKDSIFQVRKLGTDIVIIPPNYIDEVRK 120 LSQDKTRSVEPFINDFAGQYTRGMVFLQSDLQNRVIQQRLTPKLVSLTKVMKEELDYALT 180 KEMPDMKNDEWVEVDISSIMVRLISRISARVFLGPEHCRNQEWLTTTAEYSESLFITGFI 240 LRVVPHILRPFIAPLLPSYRTLLRNVSSGRRVIGDIIRSQQGDGNEDILSWMRDAATGEE 300 KQIDNIAQRMLILSLASIHTTAMTMTHAMYDLCACPEYIEPLRDEVKSVVGASGWDKTAL 360 NRFHKLDSFLKESQRFNPVFLLTFNRIYHQSMTLSDGTNIPSGTRIAVPSHAMLQDSAHV 420 PGPTPPTEFDGFRYSKIRSDSNYAQKYLFSMTDSSNMAFGYGKYACPGRFYASNEMKLTL 480 AILLLQFEFKLPDGKGRPRNITIDSDMIPDPRARLCVRKRSLRDE 525 SEQIDNO:73 ArtificialSequence aagcttaaaatggaagatcctactgtcttatatgcttgtcttgccattgcagttgcaact 60 ttcgttgttagatggtacagagatccattgagatccatcccaacagttggtggttccgat 120 ttgcctattctatcttacatcggcgcactaagatggacaagacgtggcagagagatactt 180 caagagggatatgatggctacagaggatctacattcaaaatcgcgatgttagaccgttgg 240 atcgtgatcgcaaatggtcctaaactagctgatgaagtcagacgtagaccagatgaagag 300 ttaaactttatggacggattaggagcattcgtccaaactaagtacaccttaggtgaagct 360 attcataacgatccataccatgtcgatatcataagagaaaaactaacaagaggccttcca 420 gccgtgcttcctgatgtcattgaagagttgacacttgcggttagacagtacattccaaca 480 gaaggtgatgaatgggtgtccgtaaactgttcaaaggccgcaagagatattgttgctaga 540 gcttctaatagagtctttgtaggtttgcctgcttgcagaaaccaaggttacttagatttg 600 gcaatagactttacattgtctgttgtcaaggatagagccatcatcaatatgtttccagaa 660 ttgttgaagccaatagttggcagagttgtaggtaacgccaccagaaatgttcgtagagct 720 gttccttttgttgctccattggtggaggaaagacgtagacttatggaagagtacggtgaa 780 gactggtctgaaaaacctaatgatatgttacagtggataatggatgaagctgcatccaga 840 gatagttcagtgaaggcaatcgcagagagattgttaatggtgaacttcgcggctattcat 900 acctcatcaaacactatcactcatgctttgtaccaccttgccgaaatgcctgaaactttg 960 caaccacttagagaagagatcgaaccattagtcaaagaggagggctggaccaaggctgct 1020 atgggaaaaatgtggtggttagattcatttctaagagaatctcaaagatacaatggcatt 1080 aacatcgtatctttaactagaatggctgacaaagatattacattgagtgatggcacattt 1140 ttgccaaaaggtactctagtggccgttccagcgtattctactcatagagatgatgctgtc 1200 tacgctgatgccttagtattcgatcctttcagattctcacgtatgagagcgagagaaggt 1260 gaaggtacaaagcaccagttcgttaatacttcagtcgagtacgttccatttggtcacgga 1320 aagcatgcttgtccaggaagattcttcgccgcaaacgaattgaaagcaatgttggcttac 1380 attgttctaaactatgatgtaaagttgcctggtgacggtaaacgtccattgaacatgtat 1440 tggggtccaacagttttgcctgcaccagcaggccaagtattgttcagaaagagacaagtt 1500 agtctataaccgcgg 1515 SEQIDNO:74 Trametesversicolor MEDPTVLYACLAIAVATFVVRWYRDPLRSIPTVGGSDLPILSYIGALRWTRRGREILQEG 60 YDGYRGSTFKIAMLDRWIVIANGPKLADEVRRRPDEELNFMDGLGAFVQTKYTLGEAIHN 120 DPYHVDIIREKLTRGLPAVLPDVIEELTLAVRQYIPTEGDEWVSVNCSKAARDIVARASN 180 RVFVGLPACRNQGYLDLAIDFTLSVVKDRAIINMFPELLKPIVGRVVGNATRNVRRAVPF 240 VAPLVEERRRLMEEYGEDWSEKPNDMLQWIMDEAASRDSSVKAIAERLLMVNFAAIHTSS 300 NTITHALYHLAEMPETLQPLREEIEPLVKEEGWTKAAMGKMWWLDSFLRESQRYNGINIV 360 SLTRMADKDITLSDGTFLPKGTLVAVPAYSTHRDDAVYADALVFDPFRFSRMRAREGEGT 420 KHQFVNTSVEYVPFGHGKHACPGRFFAANELKAMLAYIVLNYDVKLPGDGKRPLNMYWGP 480 TVLPAPAGQVLFRKRQVSL 499 SEQIDNO:75 ArtificialSequence atggcatttttctctatgatttcaattttgttgggatttgttatttcttctttcatcttc 60 atctttttcttcaaaaagttacttagttttagtaggaaaaacatgtcagaagtttctact 120 ttgccaagtgttccagtagtgcctggttttccagttattgggaatttgttgcaactaaag 180 gagaaaaagcctcataaaactttcactagatggtcagagatatatggacctatctactct 240 ataaagatgggttcttcatctcttattgtattgaacagtacagaaactgctaaggaagca 300 atggtcactagattttcatcaatatctaccagaaaattgtcaaacgccctaacagttcta 360 acctgcgataagtctatggtcgccacttctgattatgatgacttccacaaattagttaag 420 agatgtttgctaaatggacttcttggtgctaatgctcaaaagagaaaaagacactacaga 480 gatgctttgattgaaaatgtgagttccaagctacatgcacacgctagagatcatccacaa 540 gagccagttaactttagagcaattttcgaacacgaattgtttggtgtagcattaaagcaa 600 gccttcggtaaagacgtagaatccatatacgtcaaggagttaggcgtaacattatcaaaa 660 gatgaaatctttaaggtgcttgtacatgatatgatggagggtgcaattgatgtagattgg 720 agagatttcttcccatatttgaaatggatccctaataagtcttttgaagctaggatacaa 780 caaaagcacaagagaagactagctgttatgaacgcacttatacaggacagattgaagcaa 840 aatgggtctgaatcagatgatgattgttaccttaacttcttaatgtctgaggctaaaaca 900 ttgactaaggaacagatcgcaatccttgtctgggaaacaatcattgaaacagcagatact 960 accttagtcacaactgaatgggccatatacgagctagccaaacatccatctgtgcaagat 1020 aggttgtgtaaggagatccagaacgtgtgtggtggagagaaattcaaggaagagcagttg 1080 tcacaagttccttaccttaacggcgttttccatgaaaccttgagaaaatactcacctgca 1140 ccattagttcctattagatacgcccacgaagatacacaaatcggtggctaccatgttcca 1200 gctgggtccgaaattgctataaacatctacgggtgcaacatggacaaaaagagatgggaa 1260 agaccagaagattggtggccagaaagattcttagatgatggcaaatatgaaacatctgat 1320 ttgcataaaacaatggctttcggagctggcaaaagagtgtgtgccggtgctctacaagcc 1380 tccctaatggctggtatcgctattggtagattggtccaagagttcgaatggaaacttaga 1440 gatggtgaagaggaaaatgtcgatacttatgggttaacatctcaaaagttatacccacta 1500 atggcaatcatcaatcctagaagatcctaa 1530 SEQIDNO:76 Arabidopsisthaliana MAFFSMISILLGFVISSFIFIFFFKKLLSFSRKNMSEVSTLPSVPVVPGFPVIGNLLQLK 60 EKKPHKTFTRWSEIYGPIYSIKMGSSSLIVLNSTETAKEAMVTRFSSISTRKLSNALTVL 120 TCDKSMVATSDYDDFHKLVKRCLLNGLLGANAQKRKRHYRDALIENVSSKLHAHARDHPQ 180 EPVNFRAIFEHELFGVALKQAFGKDVESIYVKELGVTLSKDEIFKVLVHDMMEGAIDVDW 240 RDFFPYLKWIPNKSFEARIQQKHKRRLAVMNALIQDRLKQNGSESDDDCYLNFLMSEAKT 300 LTKEQIAILVWETIIETADTTLVTTEWAIYELAKHPSVQDRLCKEIQNVCGGEKFKEEQL 360 SQVPYLNGVFHETLRKYSPAPLVPIRYAHEDTQIGGYHVPAGSEIAINIYGCNMDKKRWE 420 RPEDWWPERFLDDGKYETSDLHKTMAFGAGKRVCAGALQASLMAGIAIGRLVQEFEWKLR 480 DGEEENVDTYGLTSQKLYPLMAIINPRRS 509 SEQIDNO:77 ArtificialSequence atgcaatcagattcagtcaaagtctctccatttgatttggtttccgctgctatgaatggc 60 aaggcaatggaaaagttgaacgctagtgaatctgaagatccaacaacattgcctgcacta 120 aagatgctagttgaaaatagagaattgttgacactgttcacaacttccttcgcagttctt 180 attgggtgtcttgtatttctaatgtggagacgttcatcctctaaaaagctggtacaagat 240 ccagttccacaagttatcgttgtaaagaagaaagagaaggagtcagaggttgatgacggg 300 aaaaagaaagtttctattttctacggcacacaaacaggaactgccgaaggttttgctaaa 360 gcattagtcgaggaagcaaaagtgagatatgaaaagacctctttcaaggttatcgatcta 420 gatgactacgctgcagatgatgatgaatatgaggaaaaactgaaaaaggaatccttagcc 480 ttcttcttcttggccacatacggtgatggtgaacctactgataatgctgctaacttctac 540 aagtggttcacagaaggcgacgataaaggtgaatggctgaaaaagttacaatacggagta 600 tttggtttaggtaacagacaatatgaacatttcaacaagatcgctattgtagttgatgat 660 aaacttactgaaatgggagccaaaagattagtaccagtaggattaggggatgatgatcag 720 tgtatagaagatgacttcaccgcctggaaggaattggtatggccagaattggatcaactt 780 ttaagggacgaagatgatacttctgtgactaccccatacactgcagccgtattggagtac 840 agagtggtttaccatgataaaccagcagactcatatgctgaagatcaaacccatacaaac 900 ggtcatgttgttcatgatgcacagcatccttcaagatctaatgtggctttcaaaaaggaa 960 ctacacacctctcaatcagataggtcttgtactcacttagaattcgatatttctcacaca 1020 ggactgtcttacgaaactggcgatcacgttggcgtttattccgagaacttgtccgaagtt 1080 gtcgatgaagcactaaaactgttagggttatcaccagacacatacttctcagtccatgct 1140 gataaggaggatgggacacctatcggtggtgcttcactaccaccaccttttcctccttgc 1200 acattgagagacgctctaaccagatacgcagatgtcttatcctcacctaaaaaggtagct 1260 ttgctggcattggctgctcatgctagtgatcctagtgaagccgataggttaaagttcctg 1320 gcttcaccagccggaaaagatgaatatgcacaatggatcgtcgccaaccaacgttctttg 1380 ctagaagtgatgcaaagttttccatctgccaagcctccattaggtgtgttcttcgcagca 1440 gtagctccacgtttacaaccaagatactactctatcagttcatctcctaagatgtctcct 1500 aacagaatacatgttacatgtgctttggtgtacgagactactccagcaggcagaattcac 1560 agaggattgtgttcaacctggatgaaaaatgctgtccctttaacagagtcacctgattgc 1620 tctcaagcatccattttcgttagaacatcaaatttcagacttccagtggatccaaaagtt 1680 ccagtcattatgataggaccaggcactggtcttgccccattcaggggctttcttcaagag 1740 agattggccttgaaggaatctggtacagaattgggttcttctatctttttctttggttgc 1800 cgtaatagaaaagttgactttatctacgaggacgagcttaacaattttgttgagacagga 1860 gcattgtcagaattgatcgtcgcattttcaagagaagggactgccaaagagtacgttcag 1920 cacaagatgagtcaaaaagcctccgatatatggaaacttctaagtgaaggtgcctatctt 1980 tatgtctgtggcgatgcaaagggcatggccaaggatgtccatagaactctgcatacaatt 2040 gttcaggaacaagggagtctggattcttccaaggctgaattgtacgtcaaaaacttacag 2100 atgtctggaagatacttaagagatgtttggtaa 2133 SEQIDNO:78 Steviarebaudiana MQSDSVKVSPFDLVSAAMNGKAMEKLNASESEDPTTLPALKMLVENRELLTLFTTSFAVL 60 IGCLVFLMWRRSSSKKLVQDPVPQVIVVKKKEKESEVDDGKKKVSIFYGTQTGTAEGFAK 120 ALVEEAKVRYEKTSFKVIDLDDYAADDDEYEEKLKKESLAFFFLATYGDGEPTDNAANFY 180 KWFTEGDDKGEWLKKLQYGVFGLGNRQYEHFNKIAIVVDDKLTEMGAKRLVPVGLGDDDQ 240 CIEDDFTAWKELVWPELDQLLRDEDDTSVTTPYTAAVLEYRVVYHDKPADSYAEDQTHTN 300 GHVVHDAQHPSRSNVAFKKELHTSQSDRSCTHLEFDISHTGLSYETGDHVGVYSENLSEV 360 VDEALKLLGLSPDTYFSVHADKEDGTPIGGASLPPPFPPCTLRDALTRYADVLSSPKKVA 420 LLALAAHASDPSEADRLKFLASPAGKDEYAQWIVANQRSLLEVMQSFPSAKPPLGVFFAA 480 VAPRLQPRYYSISSSPKMSPNRIHVTCALVYETTPAGRIHRGLCSTWMKNAVPLTESPDC 540 SQASIFVRTSNFRLPVDPKVPVIMIGPGTGLAPFRGFLQERLALKESGTELGSSIFFFGC 600 RNRKVDFIYEDELNNFVETGALSELIVAFSREGTAKEYVQHKMSQKASDIWKLLSEGAYL 660 YVCGDAKGMAKDVHRTLHTIVQEQGSLDSSKAELYVKNLQMSGRYLRDVW 710 SEQIDNO:79 Siraitiagrosvenorii atgaaggtcagtccattcgaattcatgtccgctattatcaagggtagaatggacccatct 60 aactcctcatttgaatctactggtgaagttgcctccgttatctttgaaaacagagaattg 120 gttgccatcttgaccacttctattgctgttatgattggttgcttcgttgtcttgatgtgg 180 agaagagctggttctagaaaggttaagaatgtcgaattgccaaagccattgattgtccat 240 gaaccagaacctgaagttgaagatggtaagaagaaggtttccatcttcttcggtactcaa 300 actggtactgctgaaggttttgctaaggctttggctgatgaagctaaagctagatacgaa 360 aaggctaccttcagagttgttgatttggatgattatgctgccgatgatgaccaatacgaa 420 gaaaaattgaagaacgaatccttcgccgttttcttgttggctacttatggtgatggtgaa 480 cctactgataatgctgctagattttacaagtggttcgccgaaggtaaagaaagaggtgaa 540 tggttgcaaaacttgcactatgctgtttttggtttgggtaacagacaatacgaacacttc 600 aacaagattgctaaggttgccgacgaattattggaagctcaaggtggtaatagattggtt 660 aaggttggtttaggtgatgacgatcaatgcatcgaagatgatttttctgcttggagagaa 720 tctttgtggccagaattggatatgttgttgagagatgaagatgatgctactactgttact 780 actccatatactgctgctgtcttggaatacagagttgtctttcatgattctgctgatgtt 840 gctgctgaagataagtcttggattaacgctaatggtcatgctgttcatgatgctcaacat 900 ccattcagatctaacgttgtcgtcagaaaagaattgcatacttctgcctctgatagatcc 960 tgttctcatttggaattcaacatttccggttccgctttgaattacgaaactggtgatcat 1020 gttggtgtctactgtgaaaacttgactgaaactgttgatgaagccttgaacttgttgggt 1080 ttgtctccagaaacttacttctctatctacaccgataacgaagatggtactccattgggt 1140 ggttcttcattgccaccaccatttccatcatgtactttgagaactgctttgaccagatac 1200 gctgatttgttgaactctccaaaaaagtctgctttgttggctttagctgctcatgcttct 1260 aatccagttgaagctgatagattgagatacttggcttctccagctggtaaagatgaatat 1320 gcccaatctgttatcggttcccaaaagtctttgttggaagttatggctgaattcccatct 1380 gctaaaccaccattaggtgttttttttgctgctgttgctccaagattgcaacctagattc 1440 tactccatttcatcctctccaagaatggctccatctagaatccatgttacttgtgctttg 1500 gtttacgataagatgccaactggtagaattcataagggtgtttgttctacctggatgaag 1560 aattctgttccaatggaaaagtcccatgaatgttcttgggctccaattttcgttagacaa 1620 tccaattttaagttgccagccgaatccaaggttccaattatcatggttggtccaggtact 1680 ggtttggctccttttagaggttttttacaagaaagattggccttgaaagaatccggtgtt 1740 gaattgggtccatccattttgtttttcggttgcagaaacagaagaatggattacatctac 1800 gaagatgaattgaacaacttcgttgaaaccggtgctttgtccgaattggttattgctttt 1860 tctagagaaggtcctaccaaagaatacgtccaacataagatggctgaaaaggcttctgat 1920 atctggaacttgatttctgaaggtgcttacttgtacgtttgtggtgatgctaaaggtatg 1980 gctaaggatgttcatagaaccttgcataccatcatgcaagaacaaggttctttggattct 2040 tccaaagctgaatccatggtcaagaacttgcaaatgaatggtagatacttaagagatgtt 2100 tggtaa 2106 SEQIDNO:80 Siraitiagrosvenorii MKVSPFEFMSAIIKGRMDPSNSSFESTGEVASVIFENRELVAILTTSIAVMIGCFVVLMW 60 RRAGSRKVKNVELPKPLIVHEPEPEVEDGKKKVSIFFGTQTGTAEGFAKALADEAKARYE 120 KATFRVVDLDDYAADDDQYEEKLKNESFAVFLLATYGDGEPTDNAARFYKWFAEGKERGE 180 WLQNLHYAVFGLGNRQYEHFNKIAKVADELLEAQGGNRLVKVGLGDDDQCIEDDFSAWRE 240 SLWPELDMLLRDEDDATTVTTPYTAAVLEYRVVFHDSADVAAEDKSWINANGHAVHDAQH 300 PFRSNVVVRKELHTSASDRSCSHLEFNISGSALNYETGDHVGVYCENLTETVDEALNLLG 360 LSPETYFSIYTDNEDGTPLGGSSLPPPFPSCTLRTALTRYADLLNSPKKSALLALAAHAS 420 NPVEADRLRYLASPAGKDEYAQSVIGSQKSLLEVMAEFPSAKPPLGVFFAAVAPRLQPRF 480 YSISSSPRMAPSRIHVTCALVYDKMPTGRIHKGVCSTWMKNSVPMEKSHECSWAPIFVRQ 540 SNFKLPAESKVPIIMVGPGTGLAPFRGFLQERLALKESGVELGPSILFFGCRNRRMDYIY 600 EDELNNFVETGALSELVIAFSREGPTKEYVQHKMAEKASDIWNLISEGAYLYVCGDAKGM 660 AKDVHRTLHTIMQEQGSLDSSKAESMVKNLQMNGRYLRDVW 701 SEQIDNO:81 ArtificialSequence atggcagaattagatacacttgatatagtagtattaggtgttatctttttgggtactgtg 60 gcatactttactaagggtaaattgtggggtgttaccaaggatccatacgctaacggattc 120 gctgcaggtggtgcttccaagcctggcagaactagaaacatcgtcgaagctatggaggaa 180 tcaggtaaaaactgtgttgttttctacggcagtcaaacaggtacagcggaggattacgca 240 tcaagacttgcaaaggaaggaaagtccagattcggtttgaacactatgatcgccgatcta 300 gaagattatgacttcgataacttagacactgttccatctgataacatcgttatgtttgta 360 ttggctacttacggtgaaggcgaaccaacagataacgccgtggatttctatgagttcatt 420 actggcgaagatgcctctttcaatgagggcaacgatcctccactaggtaacttgaattac 480 gttgcgttcggtctgggcaacaatacctacgaacactacaactcaatggtcaggaacgtt 540 aacaaggctctagaaaagttaggagctcatagaattggagaagcaggtgagggtgacgac 600 ggagctggaactatggaagaggactttttagcttggaaagatccaatgtgggaagccttg 660 gctaaaaagatgggcttggaggaaagagaagctgtatatgaacctattttcgctatcaat 720 gagagagatgatttgacccctgaagcgaatgaggtatacttgggagaacctaataagcta 780 cacttggaaggtacagcgaaaggtccattcaactcccacaacccatatatcgcaccaatt 840 gcagaatcatacgaacttttctcagctaaggatagaaattgtctgcatatggaaattgat 900 atttctggtagtaatctaaagtatgaaacaggcgaccatatcgcgatctggcctaccaac 960 ccaggtgaagaggtcaacaaatttcttgacattctagatctgtctggtaagcaacattcc 1020 gtcgtaacagtgaaagccttagaacctacagccaaagttccttttccaaatccaactacc 1080 tacgatgctatattgagataccatctggaaatatgcgctccagtttctagacagtttgtc 1140 tcaactttagcagcattcgcccctaatgatgatatcaaagctgagatgaaccgtttggga 1200 tcagacaaagattacttccacgaaaagacaggaccacattactacaatatcgctagattt 1260 ttggcctcagtctctaaaggtgaaaaatggacaaagataccattttctgctttcatagaa 1320 ggccttacaaaactacaaccaagatactattctatctcttcctctagtttagttcagcct 1380 aaaaagattagtattactgctgttgtcgaatctcagcaaattccaggtagagatgaccca 1440 ttcagaggtgtagcgactaactacttgttcgctttgaagcagaaacaaaacggtgatcca 1500 aatccagctccttttggccaatcatacgagttgacaggaccaaggaataagtatgatggt 1560 atacatgttccagtccatgtaagacattctaactttaagctaccatctgatccaggcaaa 1620 cctattatcatgatcggtccaggtaccggtgttgccccttttagaggcttcgtccaagag 1680 agggcaaaacaagccagagatggtgtagaagttggtaaaacactgctgttctttggatgt 1740 agaaagagtacagaagatttcatgtatcaaaaagagtggcaagagtacaaggaagctctt 1800 ggcgacaaattcgaaatgattacagctttttcaagagaaggatctaaaaaggtttatgtt 1860 caacacagactgaaggaaagatcaaaggaagtttctgatcttctatcccaaaaagcatac 1920 ttctacgtttgcggagacgccgcacatatggcacgtgaagtgaacactgtgttagcacag 1980 atcatagcagaaggccgtggtgtatcagaagccaagggtgaggaaattgtcaaaaacatg 2040 agatcagcaaatcaataccaagtgtgttctgatttcgtaactttacactgtaaagagaca 2100 acatacgcgaattcagaattgcaagaggatgtctggagttaa 2142 SEQIDNO:82 Gibberellafujikuroi MAELDTLDIVVLGVIFLGTVAYFTKGKLWGVTKDPYANGFAAGGASKPGRTRNIVEAMEE 60 SGKNCVVFYGSQTGTAEDYASRLAKEGKSRFGLNTMIADLEDYDFDNLDTVPSDNIVMFV 120 LATYGEGEPTDNAVDFYEFITGEDASFNEGNDPPLGNLNYVAFGLGNNTYEHYNSMVRNV 180 NKALEKLGAHRIGEAGEGDDGAGTMEEDFLAWKDPMWEALAKKMGLEEREAVYEPIFAIN 240 ERDDLTPEANEVYLGEPNKLHLEGTAKGPFNSHNPYIAPIAESYELFSAKDRNCLHMEID 300 ISGSNLKYETGDHIAIWPTNPGEEVNKFLDILDLSGKQHSVVTVKALEPTAKVPFPNPTT 360 YDAILRYHLEICAPVSRQFVSTLAAFAPNDDIKAEMNRLGSDKDYFHEKTGPHYYNIARF 420 LASVSKGEKWTKIPFSAFIEGLTKLQPRYYSISSSSLVQPKKISITAVVESQQIPGRDDP 480 FRGVATNYLFALKQKQNGDPNPAPFGQSYELTGPRNKYDGIHVPVHVRHSNFKLPSDPGK 540 PIIMIGPGTGVAPFRGFVQERAKQARDGVEVGKTLLFFGCRKSTEDFMYQKEWQEYKEAL 600 GDKFEMITAFSREGSKKVYVQHRLKERSKEVSDLLSQKAYFYVCGDAAHMAREVNTVLAQ 660 IIAEGRGVSEAKGEEIVKNMRSANQYQVCSDFVTLHCKETTYANSELQEDVWS 713 SEQIDNO:83 Steviarebaudiana atgcaatcggaatccgttgaagcatcgacgattgatttgatgactgctgttttgaaggac 60 acagtgatcgatacagcgaacgcatctgataacggagactcaaagatgccgccggcgttg 120 gcgatgatgttcgaaattcgtgatctgttgctgattttgactacgtcagttgctgttttg 180 gtcggatgtttcgttgttttggtgtggaagagatcgtccgggaagaagtccggcaaggaa 240 ttggagccgccgaagatcgttgtgccgaagaggcggctggagcaggaggttgatgatggt 300 aagaagaaggttacgattttcttcggaacacaaactggaacggctgaaggtttcgctaag 360 gcacttttcgaagaagcgaaagcgcgatatgaaaaggcagcgtttaaagtgattgatttg 420 gatgattatgctgctgatttggatgagtatgcagagaagctgaagaaggaaacatatgct 480 ttcttcttcttggctacatatggagatggtgagccaactgataatgctgccaaattttat 540 aaatggtttactgagggagacgagaaaggcgtttggcttcaaaaacttcaatatggagta 600 tttggtcttggcaacagacaatatgaacatttcaacaagattggaatagtggttgatgat 660 ggtctcaccgagcagggtgcaaaacgcattgttcccgttggtcttggagacgacgatcaa 720 tcaattgaagacgatttttcggcatggaaagagttagtgtggcccgaattggatctattg 780 cttcgcgatgaagatgacaaagctgctgcaactccttacacagctgcaatccctgaatac 840 cgcgtcgtatttcatgacaaacccgatgcgttttctgatgatcatactcaaaccaatggt 900 catgctgttcatgatgctcaacatccatgcagatccaatgtggctgttaaaaaagagctt 960 catactcctgaatccgatcgttcatgcacacatcttgaatttgacatttctcacactgga 1020 ttatcttatgaaactggggatcatgttggtgtatactgtgaaaacctaattgaagtagtg 1080 gaagaagctgggaaattgttaggattatcaacagatacttatttctcgttacatattgat 1140 aacgaagatggttcaccacttggtggaccttcattacaacctccttttcctccttgtact 1200 ttaagaaaagcattgactaattatgcagatctgttaagctctcccaaaaagtcaactttg 1260 cttgctctagctgctcatgcttccgatcccactgaagctgatcgtttaagatttcttgca 1320 tctcgcgagggcaaggatgaatatgctgaatgggttgttgcaaaccaaagaagtcttctt 1380 gaagtcatggaagctttcccgtcagctagaccgccacttggtgttttctttgcagcggtt 1440 gcaccgcgtttacagcctcgttactactctatttcttcctccccaaagatggaaccaaac 1500 aggattcatgttacttgcgcgttggtttatgaaaaaactcccgcaggtcgtatccacaaa 1560 ggaatctgctcaacctggatgaagaacgctgtacctttgaccgaaagtcaagattgcagt 1620 tgggcaccgatttttgttagaacatcaaacttcagacttccaattgacccgaaagtcccg 1680 gttatcatgattggtcctggaaccgggttggctccatttaggggttttcttcaagaaaga 1740 ttggctcttaaagaatccggaaccgaactcgggtcatctattttattcttcggttgtaga 1800 aaccgcaaagtggattacatatatgagaatgaactcaacaactttgttgaaaatggtgcg 1860 ctttctgagcttgatgttgctttctcccgcgatggcccgacgaaagaatacgtgcaacat 1920 aaaatgacccaaaaggcttctgaaatatggaatatgctttctgagggagcatatttatat 1980 gtatgtggtgatgctaaaggcatggctaaagatgtacaccgtacacttcacaccattgtg 2040 caagaacagggaagtttggactcgtctaaagcggagttgtatgtgaagaatctacaaatg 2100 tcaggaagatacctccgtgatgtttggtaa 2130 SEQIDNO:84 Steviarebaudiana MQSESVEASTIDLMTAVLKDTVIDTANASDNGDSKMPPALAMMFEIRDLLLILTTSVAVL 60 VGCFVVLVWKRSSGKKSGKELEPPKIVVPKRRLEQEVDDGKKKVTIFFGTQTGTAEGFAK 120 ALFEEAKARYEKAAFKVIDLDDYAADLDEYAEKLKKETYAFFFLATYGDGEPTDNAAKFY 180 KWFTEGDEKGVWLQKLQYGVFGLGNRQYEHFNKIGIVVDDGLTEQGAKRIVPVGLGDDDQ 240 SIEDDFSAWKELVWPELDLLLRDEDDKAAATPYTAAIPEYRVVFHDKPDAFSDDHTQTNG 300 HAVHDAQHPCRSNVAVKKELHTPESDRSCTHLEFDISHTGLSYETGDHVGVYCENLIEVV 360 EEAGKLLGLSTDTYFSLHIDNEDGSPLGGPSLQPPFPPCTLRKALTNYADLLSSPKKSTL 420 LALAAHASDPTEADRLRFLASREGKDEYAEWVVANQRSLLEVMEAFPSARPPLGVFFAAV 480 APRLQPRYYSISSSPKMEPNRIHVTCALVYEKTPAGRIHKGICSTWMKNAVPLTESQDCS 540 WAPIFVRTSNFRLPIDPKVPVIMIGPGTGLAPFRGFLQERLALKESGTELGSSILFFGCR 600 NRKVDYIYENELNNFVENGALSELDVAFSRDGPTKEYVQHKMTQKASEIWNMLSEGAYLY 660 VCGDAKGMAKDVHRTLHTIVQEQGSLDSSKAELYVKNLQMSGRYLRDVW 709 SEQIDNO:85 ArtificialSequence atgcaatctaactccgtgaagatttcgccgcttgatctggtaactgcgctgtttagcggc 60 aaggttttggacacatcgaacgcatcggaatcgggagaatctgctatgctgccgactata 120 gcgatgattatggagaatcgtgagctgttgatgatactcacaacgtcggttgctgtattg 180 atcggatgcgttgtcgttttggtgtggcggagatcgtctacgaagaagtcggcgttggag 240 ccaccggtgattgtggttccgaagagagtgcaagaggaggaagttgatgatggtaagaag 300 aaagttacggttttcttcggcacccaaactggaacagctgaaggcttcgctaaggcactt 360 gttgaggaagctaaagctcgatatgaaaaggctgtctttaaagtaattgatttggatgat 420 tatgctgctgatgacgatgagtatgaggagaaactaaagaaagaatctttggcctttttc 480 tttttggctacgtatggagatggtgagccaacagataatgctgccagattttataaatgg 540 tttactgagggagatgcgaaaggagaatggcttaataagcttcaatatggagtatttggt 600 ttgggtaacagacaatatgaacattttaacaagatcgcaaaagtggttgatgatggtctt 660 gtagaacagggtgcaaagcgtcttgttcctgttggacttggagatgatgatcaatgtatt 720 gaagatgacttcaccgcatggaaagagttagtatggccggagttggatcaattacttcgt 780 gatgaggatgacacaactgttgctactccatacacagctgctgttgcagaatatcgcgtt 840 gtttttcatgaaaaaccagacgcgctttctgaagattatagttatacaaatggccatgct 900 gttcatgatgctcaacatccatgcagatccaacgtggctgtcaaaaaggaacttcatagt 960 cctgaatctgaccggtcttgcactcatcttgaatttgacatctcgaacaccggactatca 1020 tatgaaactggggaccatgttggagtttactgtgaaaacttgagtgaagttgtgaatgat 1080 gctgaaagattagtaggattaccaccagacacttactcctccatccacactgatagtgaa 1140 gacgggtcgccacttggcggagcctcattgccgcctcctttcccgccatgcactttaagg 1200 aaagcattgacgtgttatgctgatgttttgagttctcccaagaagtcggctttgcttgca 1260 ctagctgctcatgccaccgatcccagtgaagctgatagattgaaatttcttgcatccccc 1320 gccggaaaggatgaatattctcaatggatagttgcaagccaaagaagtctccttgaagtc 1380 atggaagcattcccgtcagctaagccttcacttggtgttttctttgcatctgttgccccg 1440 cgcttacaaccaagatactactctatttcttcctcacccaagatggcaccggataggatt 1500 catgttacatgtgcattagtctatgagaaaacacctgcaggccgcatccacaaaggagtt 1560 tgttcaacttggatgaagaacgcagtgcctatgaccgagagtcaagattgcagttgggcc 1620 ccaatatacgtccgaacatccaatttcagactaccatctgaccctaaggtcccggttatc 1680 atgattggacctggcactggtttggctccttttagaggtttccttcaagagcggttagct 1740 ttaaaggaagccggaactgacctcggtttatccattttattcttcggatgtaggaatcgc 1800 aaagtggatttcatatatgaaaacgagcttaacaactttgtggagactggtgctctttct 1860 gagcttattgttgctttctcccgtgaaggcccgactaaggaatatgtgcaacacaagatg 1920 agtgagaaggcttcggatatctggaacttgctttctgaaggagcatatttatacgtatgt 1980 ggtgatgccaaaggcatggccaaagatgtacatcgaaccctccacacaattgtgcaagaa 2040 cagggatctcttgactcgtcaaaggcagaactctacgtgaagaatctacaaatgtcagga 2100 agatacctccgtgacgtttggtaa 2124 SEQIDNO:86 Steviarebaudiana MQSNSVKISPLDLVTALFSGKVLDTSNASESGESAMLPTIAMIMENRELLMILTTSVAVL 60 IGCVVVLVWRRSSTKKSALEPPVIVVPKRVQEEEVDDGKKKVTVFFGTQTGTAEGFAKAL 120 VEEAKARYEKAVFKVIDLDDYAADDDEYEEKLKKESLAFFFLATYGDGEPTDNAARFYKW 180 FTEGDAKGEWLNKLQYGVFGLGNRQYEHFNKIAKVVDDGLVEQGAKRLVPVGLGDDDQCI 240 EDDFTAWKELVWPELDQLLRDEDDTTVATPYTAAVAEYRVVFHEKPDALSEDYSYTNGHA 300 VHDAQHPCRSNVAVKKELHSPESDRSCTHLEFDISNTGLSYETGDHVGVYCENLSEVVND 360 AERLVGLPPDTYSSIHTDSEDGSPLGGASLPPPFPPCTLRKALTCYADVLSSPKKSALLA 420 LAAHATDPSEADRLKFLASPAGKDEYSQWIVASQRSLLEVMEAFPSAKPSLGVFFASVAP 480 RLQPRYYSISSSPKMAPDRIHVTCALVYEKTPAGRIHKGVCSTWMKNAVPMTESQDCSWA 540 PIYVRTSNFRLPSDPKVPVIMIGPGTGLAPFRGFLQERLALKEAGTDLGLSILFFGCRNR 600 KVDFIYENELNNFVETGALSELIVAFSREGPTKEYVQHKMSEKASDIWNLLSEGAYLYVC 660 GDAKGMAKDVHRTLHTIVQEQGSLDSSKAELYVKNLQMSGRYLRDVW 707 SEQIDNO:87 ArtificialSequence atgtcctccaactccgatttggtcagaagattggaatctgttttgggtgtttctttcggt 60 ggttctgttactgattccgttgttgttattgctaccacctctattgctttggttatcggt 120 gttttggttttgttgtggagaagatcctctgacagatctagagaagttaagcaattggct 180 gttccaaagccagttactatcgttgaagaagaagatgaattcgaagttgcttctggtaag 240 accagagtttctattttctacggtactcaaactggtactgctgaaggttttgctaaggct 300 ttggctgaagaaatcaaagccagatacgaaaaagctgccgttaaggttattgatttggat 360 gattacacagccgaagatgacaaatacggtgaaaagttgaagaaagaaactatggccttc 420 ttcatgttggctacttatggtgatggtgaacctactgataatgctgctagattttacaag 480 tggttcaccgaaggtactgatagaggtgtttggttggaacatttgagatacggtgtattc 540 ggtttgggtaacagacaatacgaacacttcaacaagattgccaaggttgttgatgatttg 600 ttggttgaacaaggtgccaagagattggttactgttggtttgggtgatgatgatcaatgc 660 atcgaagatgatttctccgcttggaaagaagccttgtggccagaattggatcaattattg 720 caagatgataccaacaccgtttctactccatacactgctgttattccagaatacagagtt 780 gttatccacgatccatctgttacctcttatgaagatccatactctaacatggctaacggt 840 aatgcctcttacgatattcatcatccatgtagagctaacgttgccgtccaaaaagaattg 900 cataagccagaatctgacagaagttgcatccatttggaattcgatattttcgctactggt 960 ttgacttacgaaaccggtgatcatgttggtgtttacgctgataattgtgatgatactgta 1020 gaagaagccgctaagttgttgggtcaaccattggatttgttgttctccattcataccgat 1080 aacaacgacggtacttctttgggttcttctttgccaccaccatttccaggtccatgtact 1140 ttgagaactgctttggctagatatgccgatttgttgaatccaccaaaaaaggctgctttg 1200 attgctttagctgctcatgctgatgaaccatctgaagctgaaagattgaagttcttgtca 1260 tctccacaaggtaaggacgaatattctaaatgggttgtcggttcccaaagatccttggtt 1320 gaagttatggctgaatttccatctgctaaaccaccattgggtgtattttttgctgctgtt 1380 gttcctagattgcaacctagatattactccatctcttccagtccaagatttgctccacat 1440 agagttcatgttacttgcgctttggtttatggtccaactccaactggtagaattcacaga 1500 ggtgtatgttcattctggatgaagaatgttgtcccattggaaaagtctcaaaactgttct 1560 tgggccccaattttcatcagacaatctaatttcaagttgccagccgatcattctgttcca 1620 atagttatggttggtccaggtactggtttagctccttttagaggtttcttacaagaaaga 1680 ttggccttgaaagaagaaggtgctcaagttggtcctgctttgttgttttttggttgcaga 1740 aacagacaaatggacttcatctacgaagtcgaattgaacaactttgtcgaacaaggtgct 1800 ttgtccgaattgatcgttgctttttcaagagaaggtccatccaaagaatacgtccaacat 1860 aagatggttgaaaaggcagcttacatgtggaacttgatttctcaaggtggttacttctac 1920 gtttgtggtgatgctaaaggtatggctagagatgttcatagaacattgcataccatcgtc 1980 caacaagaagaaaaggttgattctaccaaggccgaatccatcgttaagaaattgcaaatg 2040 gacggtagatacttgagagatgtttggtga 2070 SEQIDNO:88 Rubussuavissimus MSSNSDLVRRLESVLGVSFGGSVTDSVVVIATTSIALVIGVLVLLWRRSSDRSREVKQLA 60 VPKPVTIVEEEDEFEVASGKTRVSIFYGTQTGTAEGFAKALAEEIKARYEKAAVKVIDLD 120 DYTAEDDKYGEKLKKETMAFFMLATYGDGEPTDNAARFYKWFTEGTDRGVWLEHLRYGVF 180 GLGNRQYEHFNKIAKVVDDLLVEQGAKRLVTVGLGDDDQCIEDDFSAWKEALWPELDQLL 240 QDDTNTVSTPYTAVIPEYRVVIHDPSVTSYEDPYSNMANGNASYDIHHPCRANVAVQKEL 300 HKPESDRSCIHLEFDIFATGLTYETGDHVGVYADNCDDTVEEAAKLLGQPLDLLFSIHTD 360 NNDGTSLGSSLPPPFPGPCTLRTALARYADLLNPPKKAALIALAAHADEPSEAERLKFLS 420 SPQGKDEYSKWVVGSQRSLVEVMAEFPSAKPPLGVFFAAVVPRLQPRYYSISSSPRFAPH 480 RVHVTCALVYGPTPTGRIHRGVCSFWMKNVVPLEKSQNCSWAPIFIRQSNFKLPADHSVP 540 IVMVGPGTGLAPFRGFLQERLALKEEGAQVGPALLFFGCRNRQMDFIYEVELNNFVEQGA 600 LSELIVAFSREGPSKEYVQHKMVEKAAYMWNLISQGGYFYVCGDAKGMARDVHRTLHTIV 660 QQEEKVDSTKAESIVKKLQMDGRYLRDVW 689 SEQIDNO:89 ArtificialSequence atgacttctgcactttatgcctccgatcttttcaaacaattgaaaagtatcatgggaacg 60 gattctttgtccgatgatgttgtattagttattgctacaacttctctggcactggttgct 120 ggtttcgttgtcttattgtggaaaaagaccacggcagatcgttccggcgagctaaagcca 180 ctaatgatccctaagtctctgatggcgaaagatgaggatgatgacttagatctaggttct 240 ggaaaaacgagagtctctatcttcttcggcacacaaaccggaacagccgaaggattcgct 300 aaagcactttcagaagagatcaaagcaagatacgaaaaggcggctgtaaaagtaatcgat 360 ttggatgattacgctgccgatgatgaccaatatgaggaaaagttgaaaaaggaaacattg 420 gctttcttttgtgtagccacgtatggtgatggtgaaccaaccgataacgccgcaagattc 480 tacaagtggtttactgaagagaacgaaagagatatcaagttgcagcaacttgcttacggc 540 gtttttgccttaggtaacagacaatacgagcactttaacaagataggtattgtcttagat 600 gaagagttatgcaaaaagggtgcgaagagattgattgaagtcggtttaggagatgatgat 660 caatctatcgaggatgactttaatgcatggaaggaatctttgtggtctgaattagataag 720 ttacttaaggacgaagatgataaatccgttgccactccatacacagccgtcattccagaa 780 tatagagtagttactcatgatccaagattcacaacacagaaatcaatggaaagtaatgtg 840 gctaatggtaatactaccatcgatattcatcatccatgtagagtagacgttgcagttcaa 900 aaggaattgcacactcatgaatcagacagatcttgcatacatcttgaatttgatatatca 960 cgtactggtatcacttacgaaacaggtgatcacgtgggtgtctacgctgaaaaccatgtt 1020 gaaattgtagaggaagctggaaagttgttgggccatagtttagatcttgttttctcaatt 1080 catgccgataaagaggatggctcaccactagaaagtgcagtgcctccaccatttccagga 1140 ccatgcaccctaggtaccggtttagctcgttacgcggatctgttaaatcctccacgtaaa 1200 tcagctctagtggccttggctgcgtacgccacagaaccttctgaggcagaaaaactgaaa 1260 catctaacttcaccagatggtaaggatgaatactcacaatggatagtagctagtcaacgt 1320 tctttactagaagttatggctgctttcccatccgctaaacctcctttgggtgttttcttc 1380 gccgcaatagcgcctagactgcaaccaagatactattcaatttcatcctcacctagactg 1440 gcaccatcaagagttcatgtcacatccgctttagtgtacggtccaactcctactggtaga 1500 atccataagggcgtttgttcaacatggatgaaaaacgcggttccagcagagaagtctcac 1560 gaatgttctggtgctccaatctttatcagagcctccaacttcaaactgccttccaatcct 1620 tctactcctattgtcatggtcggtcctggtacaggtcttgctccattcagaggtttctta 1680 caagagagaatggccttaaaggaggatggtgaagagttgggatcttctttgttgtttttc 1740 ggctgtagaaacagacaaatggatttcatctacgaagatgaactgaataactttgtagat 1800 caaggagttatttcagagttgataatggctttttctagagaaggtgctcagaaggagtac 1860 gtccaacacaaaatgatggaaaaggccgcacaagtttgggacttaatcaaagaggaaggc 1920 tatctatatgtctgtggtgatgcaaagggtatggcaagagatgttcacagaacacttcat 1980 actatagtccaggaacaggaaggcgttagttcttctgaagcggaagcaattgtgaaaaag 2040 ttacaaacagagggaagatacttgagagatgtgtggtaa 2079 SEQIDNO:90 Arabidopsisthaliana MTSALYASDLFKQLKSIMGTDSLSDDVVLVIATTSLALVAGFVVLLWKKTTADRSGELKP 60 LMIPKSLMAKDEDDDLDLGSGKTRVSIFFGTQTGTAEGFAKALSEEIKARYEKAAVKVID 120 LDDYAADDDQYEEKLKKETLAFFCVATYGDGEPTDNAARFYKWFTEENERDIKLQQLAYG 180 VFALGNRQYEHFNKIGIVLDEELCKKGAKRLIEVGLGDDDQSIEDDFNAWKESLWSELDK 240 LLKDEDDKSVATPYTAVIPEYRVVTHDPRFTTQKSMESNVANGNTTIDIHHPCRVDVAVQ 300 KELHTHESDRSCIHLEFDISRTGITYETGDHVGVYAENHVEIVEEAGKLLGHSLDLVFSI 360 HADKEDGSPLESAVPPPFPGPCTLGTGLARYADLLNPPRKSALVALAAYATEPSEAEKLK 420 HLTSPDGKDEYSQWIVASQRSLLEVMAAFPSAKPPLGVFFAAIAPRLQPRYYSISSSPRL 480 APSRVHVTSALVYGPTPTGRIHKGVCSTWMKNAVPAEKSHECSGAPIFIRASNFKLPSNP 540 STPIVMVGPGTGLAPFRGFLQERMALKEDGEELGSSLLFFGCRNRQMDFIYEDELNNFVD 600 QGVISELIMAFSREGAQKEYVQHKMMEKAAQVWDLIKEEGYLYVCGDAKGMARDVHRTLH 660 TIVQEQEGVSSSEAEAIVKKLQTEGRYLRDVW 692 SEQIDNO:91 ArtificialSequence atgtcttcctcttcctcttccagtacctctatgattgatttgatggctgctattattaaa 60 ggtgaaccagttatcgtctccgacccagcaaatgcctctgcttatgaatcagttgctgca 120 gaattgtcttcaatgttgatcgaaaacagacaattcgccatgatcgtaactacatcaatc 180 gctgttttgatcggttgtattgtcatgttggtatggagaagatccggtagtggtaattct 240 aaaagagtcgaacctttgaaaccattagtaattaagccaagagaagaagaaatagatgac 300 ggtagaaagaaagttacaatatttttcggtacccaaactggtacagctgaaggttttgca 360 aaagccttaggtgaagaagctaaggcaagatacgaaaagactagattcaagatagtcgat 420 ttggatgactatgccgctgatgacgatgaatacgaagaaaagttgaagaaagaagatgtt 480 gcatttttctttttggcaacctatggtgacggtgaaccaactgacaatgcagccagattc 540 tacaaatggtttacagagggtaatgatcgtggtgaatggttgaaaaacttaaagtacggt 600 gttttcggtttgggtaacagacaatacgaacatttcaacaaagttgcaaaggttgtcgac 660 gatattttggtcgaacaaggtgctcaaagattagtccaagtaggtttgggtgacgatgac 720 caatgtatagaagatgactttactgcctggagagaagctttgtggcctgaattagacaca 780 atcttgagagaagaaggtgacaccgccgttgctaccccatatactgctgcagtattagaa 840 tacagagtttccatccatgatagtgaagacgcaaagtttaatgatatcactttggccaat 900 ggtaacggttatacagttttcgatgcacaacacccttacaaagctaacgttgcagtcaag 960 agagaattacatacaccagaatccgacagaagttgtatacacttggaatttgatatcgct 1020 ggttccggtttaaccatgaagttgggtgaccatgtaggtgttttatgcgacaatttgtct 1080 gaaactgttgatgaagcattgagattgttggatatgtcccctgacacttattttagtttg 1140 cacgctgaaaaagaagatggtacaccaatttccagttctttaccacctccattccctcca 1200 tgtaacttaagaacagccttgaccagatacgcttgcttgttatcatcccctaaaaagtcc 1260 gccttggttgctttagccgctcatgctagtgatcctactgaagcagaaagattgaaacac 1320 ttagcatctccagccggtaaagatgaatattcaaagtgggtagttgaatctcaaagatca 1380 ttgttagaagttatggcagaatttccatctgccaagcctccattaggtgtcttctttgct 1440 ggtgtagcacctagattgcaaccaagattctactcaatcagttcttcacctaagatcgct 1500 gaaactagaattcatgttacatgtgcattagtctacgaaaagatgccaaccggtagaatt 1560 cacaagggtgtatgctctacttggatgaaaaatgctgttccttacgaaaaatcagaaaag 1620 ttgttcttaggtagaccaatcttcgtaagacaatcaaacttcaagttgccttctgattca 1680 aaggttccaataatcatgataggtcctggtacaggtttagccccattcagaggtttcttg 1740 caagaaagattggctttagttgaatctggtgtcgaattaggtccttcagttttgttcttt 1800 ggttgtagaaacagaagaatggatttcatctatgaagaagaattgcaaagattcgtcgaa 1860 tctggtgcattggccgaattatctgtagctttttcaagagaaggtccaactaaggaatac 1920 gttcaacataagatgatggataaggcatccgacatatggaacatgatcagtcaaggtgct 1980 tatttgtacgtttgcggtgacgcaaagggtatggccagagatgtccatagatctttgcac 2040 acaattgctcaagaacaaggttccatggatagtaccaaagctgaaggtttcgtaaagaac 2100 ttacaaacttccggtagatacttgagagatgtctggtga 2139 SEQIDNO:92 Arabidopsisthaliana MSSSSSSSTSMIDLMAAIIKGEPVIVSDPANASAYESVAAELSSMLIENRQFAMIVTTSI 60 AVLIGCIVMLVWRRSGSGNSKRVEPLKPLVIKPREEEIDDGRKKVTIFFGTQTGTAEGFA 120 KALGEEAKARYEKTRFKIVDLDDYAADDDEYEEKLKKEDVAFFFLATYGDGEPTDNAARF 180 YKWFTEGNDRGEWLKNLKYGVFGLGNRQYEHFNKVAKVVDDILVEQGAQRLVQVGLGDDD 240 QCIEDDFTAWREALWPELDTILREEGDTAVATPYTAAVLEYRVSIHDSEDAKFNDITLAN 300 GNGYTVFDAQHPYKANVAVKRELHTPESDRSCIHLEFDIAGSGLTMKLGDHVGVLCDNLS 360 ETVDEALRLLDMSPDTYFSLHAEKEDGTPISSSLPPPFPPCNLRTALTRYACLLSSPKKS 420 ALVALAAHASDPTEAERLKHLASPAGKDEYSKWVVESQRSLLEVMAEFPSAKPPLGVFFA 480 GVAPRLQPRFYSISSSPKIAETRIHVTCALVYEKMPTGRIHKGVCSTWMKNAVPYEKSEK 540 LFLGRPIFVRQSNFKLPSDSKVPIIMIGPGTGLAPFRGFLQERLALVESGVELGPSVLFF 600 GCRNRRMDFIYEEELQRFVESGALAELSVAFSREGPTKEYVQHKMMDKASDIWNMISQGA 660 YLYVCGDAKGMARDVHRSLHTIAQEQGSMDSTKAEGFVKNLQTSGRYLRDVW 712 SEQIDNO:93 ArtificialSequence atggaagcctcttacctatacatttctattttgcttttactggcatcatacctgttcacc 60 actcaacttagaaggaagagcgctaatctaccaccaaccgtgtttccatcaataccaatc 120 attggacacttatacttactcaaaaagcctctttatagaactttagcaaaaattgccgct 180 aagtacggaccaatactgcaattacaactcggctacagacgtgttctggtgatttcctca 240 ccatcagcagcagaagagtgctttaccaataacgatgtaatcttcgcaaatagacctaag 300 acattgtttggcaaaatagtgggtggaacatcccttggcagtttatcctacggcgatcaa 360 tggcgtaatctaaggagagtagcttctatcgaaatcctatcagttcataggttgaacgaa 420 tttcatgatatcagagtggatgagaacagattgttaattagaaaacttagaagttcatct 480 tctcctgttactcttataacagtcttttatgctctaacattgaacgtcattatgagaatg 540 atctctggcaaaagatatttcgacagtggggatagagaattggaggaggaaggtaagaga 600 tttcgagaaatcttagacgaaacgttgcttctagccggtgcttctaatgttggcgactac 660 ttaccaatattgaactggttgggagttaagtctcttgaaaagaaattgatcgctttgcag 720 aaaaagagagatgactttttccagggtttgattgaacaggttagaaaatctcgtggtgct 780 aaagtaggcaaaggtagaaaaacgatgatcgaactcttattatctttgcaagagtcagaa 840 cctgagtactatacagatgctatgataagatcttttgtcctaggtctgctggctgcaggt 900 agtgatacttcagcgggcactatggaatgggccatgagcttactggtcaatcacccacat 960 gtattgaagaaagctcaagctgaaatcgatagagttatcggtaataacagattgattgac 1020 gagtcagacattggaaatatcccttacatcgggtgtattatcaatgaaactctaagactc 1080 tatccagcagggccattgttgttcccacatgaaagttctgccgactgcgttatttccggt 1140 tacaatatacctagaggtacaatgttaatcgtaaaccaatgggcgattcatcacgatcct 1200 aaagtctgggatgatcctgaaacctttaaacctgaaagatttcaaggattagaaggaact 1260 agagatggtttcaaacttatgccattcggttctgggagaagaggatgtccaggtgaaggt 1320 ttggcaataaggctgttagggatgacactaggctcagtgatccaatgttttgattgggag 1380 agagtaggagatgagatggttgacatgacagaaggtttgggtgtcacacttcctaaggcc 1440 gttccattagttgccaaatgtaagccacgttccgaaatgactaatctcctatccgaactt 1500 taa 1503 SEQIDNO:94 S.rebaudiana MEASYLYISILLLLASYLFTTQLRRKSANLPPTVFPSIPIIGHLYLLKKPLYRTLAKIAA 60 KYGPILQLQLGYRRVLVISSPSAAEECFTNNDVIFANRPKTLFGKIVGGTSLGSLSYGDQ 120 WRNLRRVASIEILSVHRLNEFHDIRVDENRLLIRKLRSSSSPVTLITVFYALTLNVIMRM 180 ISGKRYFDSGDRELEEEGKRFREILDETLLLAGASNVGDYLPILNWLGVKSLEKKLIALQ 240 KKRDDFFQGLIEQVRKSRGAKVGKGRKTMIELLLSLQESEPEYYTDAMIRSFVLGLLAAG 300 SDTSAGTMEWAMSLLVNHPHVLKKAQAEIDRVIGNNRLIDESDIGNIPYIGCIINETLRL 360 YPAGPLLFPHESSADCVISGYNIPRGTMLIVNQWAIHHDPKVWDDPETFKPERFQGLEGT 420 RDGFKLMPFGSGRRGCPGEGLAIRLLGMTLGSVIQCFDWERVGDEMVDMTEGLGVTLPKA 480 VPLVAKCKPRSEMTNLLSEL 500 SEQIDNO:95 Rubussuavissimus atggaagtaacagtagctagtagtgtagccctgagcctggtctttattagcatagtagta 60 agatgggcatggagtgtggtgaattgggtgtggtttaagccgaagaagctggaaagattt 120 ttgagggagcaaggccttaaaggcaattcctacaggtttttatatggagacatgaaggag 180 aactctatcctgctcaaacaagcaagatccaaacccatgaacctctccacctcccatgac 240 atagcacctcaagtcaccccttttgtcgaccaaaccgtgaaagcttacggtaagaactct 300 tttaattgggttggccccataccaagggtgaacataatgaatccagaagatttgaaggac 360 gtcttaacaaaaaatgttgactttgttaagccaatatcaaacccacttatcaagttgcta 420 gctacaggtattgcaatctatgaaggtgagaaatggactaaacacagaaggattatcaac 480 ccaacattccattcggagaggctaaagcgtatgttaccttcatttcaccaaagttgtaat 540 gagatggtcaaggaatgggagagcttggtgtcaaaagagggttcatcatgtgagttggat 600 gtctggccttttcttgaaaatatgtcggcagatgtgatctcgagaacagcatttggaact 660 agctacaaaaaaggacagaaaatctttgaactcttgagagagcaagtaatatatgtaacg 720 aaaggctttcaaagtttttacattccaggatggaggtttctcccaactaagatgaacaag 780 aggatgaatgagattaacgaagaaataaaaggattaatcaggggtattataattgacaga 840 gagcaaatcattaaggcaggtgaagaaaccaacgatgacttattaggtgcacttatggag 900 tcaaacttgaaggacattcgggaacatgggaaaaacaacaaaaatgttgggatgagtatt 960 gaagatgtaattcaggagtgtaagctgttttactttgctgggcaagaaaccacttcagtg 1020 ttgctggcttggacaatggttttacttggtcaaaatcagaactggcaagatcgagcaaga 1080 caagaggttttgcaagtctttggaagcagcaagccagattttgatggtctagctcacctt 1140 aaagtcgtaaccatgattttgcttgaagttcttcgattatacccaccagtcattgaactt 1200 attcgaaccattcacaagaaaacacaacttgggaagctctcactaccagaaggagttgaa 1260 gtccgcttaccaacactgctcattcaccatgacaaggaactgtggggtgatgatgcaaac 1320 cagttcaatccagagaggttttcggaaggagtttccaaagcaacaaagaaccgactctca 1380 ttcttccccttcggagccggtccacgcatttgcattggacagaacttttctatgatggaa 1440 gcaaagttggccttagcattgatcttgcaacacttcacctttgagctttctccatctcat 1500 gcacatgctccttcccatcgtataacccttcaaccacagtatggtgttcgtatcatttta 1560 catcgacgttag 1572 SEQIDNO:96 ArtificialSequence atggaagtcactgtcgcctcttctgtcgctttatccttagtcttcatttccattgtcgtc 60 agatgggcttggtccgttgtcaactgggtttggttcaaaccaaagaagttggaaagattc 120 ttgagagagcaaggtttgaagggtaattcttatagattcttgtacggtgacatgaaggaa 180 aattctattttgttgaagcaagccagatccaaaccaatgaacttgtctacctctcatgat 240 attgctccacaagttactccattcgtcgatcaaactgttaaagcctacggtaagaactct 300 ttcaattgggttggtccaattcctagagttaacatcatgaacccagaagatttgaaggat 360 gtcttgaccaagaacgttgacttcgttaagccaatttccaacccattgattaaattgttg 420 gctactggtattgccatttacgaaggtgaaaagtggactaagcatagaagaatcatcaac 480 cctaccttccactctgaaagattgaagagaatgttaccatctttccatcaatcctgtaat 540 gaaatggttaaggaatgggaatccttggtttctaaagaaggttcttcttgcgaattggat 600 gtttggccattcttggaaaatatgtctgctgatgtcatttccagaaccgctttcggtacc 660 tcctacaagaagggtcaaaagattttcgaattgttgagagagcaagttatttacgttacc 720 aagggtttccaatccttctacatcccaggttggagattcttgccaactaaaatgaacaag 780 cgtatgaacgagatcaacgaagaaattaaaggtttgatcagaggtattattatcgacaga 840 gaacaaattattaaagctggtgaagaaaccaacgatgatttgttgggtgctttgatggag 900 tccaacttgaaggatattagagaacatggtaagaacaacaagaatgttggtatgtctatt 960 gaagatgttattcaagaatgtaagttattctacttcgctggtcaagagaccacttctgtt 1020 ttgttagcctggactatggtcttgttaggtcaaaaccaaaattggcaagatagagctaga 1080 caagaagttttgcaagtcttcggttcttccaagccagactttgatggtttggcccacttg 1140 aaggttgttactatgattttgttagaagttttgagattgtacccaccagtcattgagtta 1200 atcagaaccattcataaaaagactcaattgggtaaattatctttgccagaaggtgttgaa 1260 gtcagattaccaaccttgttgattcaccacgataaggaattatggggtgacgacgctaat 1320 caatttaatccagaaagattttccgaaggtgtttccaaggctaccaaaaaccgtttgtcc 1380 ttcttcccatttggtgctggtccacgtatttgtatcggtcaaaacttttccatgatggaa 1440 gccaagttggctttggctttaatcttgcaacacttcactttcgaattgtctccatcccat 1500 gcccacgctccttctcatagaatcactttacaaccacaatacggtgtcagaatcatctta 1560 cacagaagataa 1572 SEQIDNO:97 Rubussuavissimus MEVTVASSVALSLVFISIVVRWAWSVVNWVWFKPKKLERFLREQGLKGNSYRFLYGDMKE 60 NSILLKQARSKPMNLSTSHDIAPQVTPFVDQTVKAYGKNSFNWVGPIPRVNIMNPEDLKD 120 VLTKNVDFVKPISNPLIKLLATGIAIYEGEKWTKHRRIINPTFHSERLKRMLPSFHQSCN 180 EMVKEWESLVSKEGSSCELDVWPFLENMSADVISRTAFGTSYKKGQKIFELLREQVIYVT 240 KGFQSFYIPGWRFLPTKMNKRMNEINEEIKGLIRGIIIDREQIIKAGEETNDDLLGALME 300 SNLKDIREHGKNNKNVGMSIEDVIQECKLFYFAGQETTSVLLAWTMVLLGQNQNWQDRAR 360 QEVLQVFGSSKPDFDGLAHLKVVTMILLEVLRLYPPVIELIRTIHKKTQLGKLSLPEGVE 420 VRLPTLLIHHDKELWGDDANQFNPERFSEGVSKATKNRLSFFPFGAGPRICIGQNFSMME 480 AKLALALILQHFTFELSPSHAHAPSHRITLQPQYGVRIILHRR 523 SEQIDNO:98 Prunusavium atggaagcatcaagggctagttgtgttgcgctatgtgttgtttgggtgagcatagtaatt 60 acattggcatggagggtgctgaattgggtgtggttgaggccaaagaaactagaaagatgc 120 ttgagggagcaaggccttacaggcaattcttacaggcttttgtttggagacaccaaggat 180 ctctcgaagatgctggaacaaacacaatccaaacccatcaaactctccacctcccatgat 240 atagcgccacgagtcaccccatttttccatcgaactgtgaactctaatggcaagaattct 300 tttgtttggatgggccctataccaagagtgcacatcatgaatccagaagatttgaaagat 360 gccttcaacagacatgatgattttcataagacagtaaaaaatcctatcatgaagtctcca 420 ccaccgggcattgtaggcattgaaggtgagcaatgggctaaacacagaaagattatcaac 480 ccagcattccatttagagaagctaaagggtatggtaccaatattttaccaaagttgtagc 540 gagatgattaacaaatgggagagcttggtgtccaaagagagttcatgtgagttggatgtg 600 tggccttatcttgaaaattttaccagcgatgtgatttcccgagctgcatttggaagtagc 660 tatgaagagggaaggaaaatatttcaactactaagagaggaagcaaaagtttattcggta 720 gctctacgaagtgtttacattccaggatggaggtttctaccaaccaagcagaacaagaag 780 acgaaggaaattcacaatgaaattaaaggcttacttaagggcattataaataaaagggaa 840 gaggcgatgaaggcaggggaagccactaaagatgacttactaggaatacttatggagtcc 900 aacttcagggaaattcaggaacatgggaacaacaaaaatgctggaatgagtattgaagat 960 gtaattggagagtgtaagttgttttactttgctgggcaagagaccacttcggtgttgctt 1020 gtttggacaatgattttactaagccaaaatcaggattggcaagctcgtgcaagagaagag 1080 gtcttgaaagtctttggaagcaacatcccaacctatgaagagctaagtcacctaaaagtt 1140 gtgaccatgattttacttgaagttcttcgattatacccatcagtcgttgcgcttcctcga 1200 accactcacaagaaaacacagcttggaaaattatcattaccagctggagtggaagtctcc 1260 ttgcccatactgcttgttcaccatgacaaagagttgtggggtgaggatgcaaatgagttc 1320 aagccagagaggttttcagagggagtttcaaaggcaacaaagaacaaatttacatactta 1380 cctttcggagggggtccaaggatttgcattggacaaaactttgccatggtggaagctaaa 1440 ttggccttggccctgattttacaacactttgcctttgagctttctccatcctatgctcat 1500 gctccttctgcagttataacccttcaacctcaatttggtgctcatatcattttgcataaa 1560 cgttga 1566 SEQIDNO:99 ArtificialSequence atggaagcttctagagcatcttgtgttgctttgtgtgttgtttgggtttccatcgttatt 60 actttggcttggagagttttgaattgggtctggttaagaccaaaaaagttggaaagatgc 120 ttgagagaacaaggtttgactggtaactcttacagattgttgttcggtgataccaaggac 180 ttgtctaagatgttggaacaaactcaatccaagcctatcaagttgtctacctctcatgat 240 attgctccaagagttactccattcttccatagaactgttaactccaacggtaagaactct 300 tttgtttggatgggtccaattccaagagtccatattatgaaccctgaagatttgaaggac 360 gctttcaacagacatgatgatttccataagaccgtcaagaacccaattatgaagtctcca 420 ccaccaggtatagttggtattgaaggtgaacaatgggccaaacatagaaagattattaac 480 ccagccttccacttggaaaagttgaaaggtatggttccaatcttctaccaatcctgctct 540 gaaatgattaacaagtgggaatccttggtttccaaagaatcttcctgtgaattggatgtc 600 tggccatatttggaaaacttcacctccgatgttatttccagagctgcttttggttcttct 660 tacgaagaaggtagaaagatcttccaattattgagagaagaagccaaggtttactccgtt 720 gctttgagatctgtttacattccaggttggagattcttgccaactaagcaaaacaaaaag 780 accaaagaaatccacaacgaaatcaagggtttgttgaagggtatcatcaacaagagagaa 840 gaagctatgaaggctggtgaagctacaaaagatgatttgttgggtatcttgatggaatcc 900 aacttcagagaaatccaagaacacggtaacaacaagaatgccggtatgtctattgaagat 960 gttatcggtgaatgcaagttgttctactttgctggtcaagaaactacctccgttttgttg 1020 gtttggaccatgattttgttgtcccaaaatcaagattggcaagctagagctagagaagaa 1080 gtcttgaaagttttcggttctaacatcccaacctacgaagaattgtctcacttgaaggtt 1140 gtcactatgatcttgttggaagtattgagattatacccatccgttgttgcattgccaaga 1200 actactcataagaaaactcaattgggtaaattgtccttgccagctggtgttgaagtttct 1260 ttgccaattttgttagtccaccacgacaaagaattgtggggtgaagatgctaatgaattc 1320 aagccagaaagattctccgaaggtgtttctaaagctaccaagaacaagttcacttacttg 1380 ccatttggtggtggtccaagaatatgtattggtcaaaatttcgctatggtcgaagctaaa 1440 ttggctttggctttgatcttgcaacatttcgctttcgaattgtcaccatcttatgctcat 1500 gctccatctgctgttattacattgcaaccacaatttggtgcccatatcatcttgcataag 1560 agataac 1567 SEQIDNO:100 Prunusavium MEASRASCVALCVVWVSIVITLAWRVLNWVWLRPKKLERCLREQGLTGNSYRLLFGDTKD 60 LSKMLEQTQSKPIKLSTSHDIAPRVTPFFHRTVNSNGKNSFVWMGPIPRVHIMNPEDLKD 120 AFNRHDDFHKTVKNPIMKSPPPGIVGIEGEQWAKHRKIINPAFHLEKLKGMVPIFYQSCS 180 EMINKWESLVSKESSCELDVWPYLENFTSDVISRAAFGSSYEEGRKIFQLLREEAKVYSV 240 ALRSVYIPGWRFLPTKQNKKTKEIHNEIKGLLKGIINKREEAMKAGEATKDDLLGILMES 300 NFREIQEHGNNKNAGMSIEDVIGECKLFYFAGQETTSVLLVWTMILLSQNQDWQARAREE 360 VLKVFGSNIPTYEELSHLKVVTMILLEVLRLYPSVVALPRTTHKKTQLGKLSLPAGVEVS 420 LPILLVHHDKELWGEDANEFKPERFSEGVSKATKNKFTYLPFGGGPRICIGQNFAMVEAK 480 LALALILQHFAFELSPSYAHAPSAVITLQPQFGAHIILHKR 521 SEQIDNO:101 Prunusmume ASWVAVLSVVWVSMVIAWAWRVLNWVWLRPKKLEKCLREQGLAGNSYRLLFGDTKDLSKM 60 LEQTQSKPIKLSTSHDIAPHVTPFFHQTVNSYGKNSFVWMGPIPRVHIMNPEDLKDTFNR 120 HDDFHKVVKNPIMKSLPQGIVGIEGEQWAKHRKIINPAFHLEKLKGMVPIFYRSCSEMIN 180 KWESLVSKESSCELDVWPYLENFTSDVISRAAFGSSYEEGRKIFQLLREEAKIYTVAMRS 240 VYIPGWRFLPTKQNKKAKEIHNEIKGLLKGIINKREEAMKAGEATKDDLLGILMESNFRE 300 IQEHGNNKNAGMSIEDVIGECKLFYFAGQETTSVLLVWTMVLLSQNQDWQARAREEVLQV 360 FGSNIPTYEELSQLKVVTMILLEVLRLYPSVVALPRTTHKKTQLGKLSLPAGVEVSLPIL 420 LVHHDKELWGEDANEFKPERFSEGVSKATKNQFTYFPFGGGPRICIGQNFAMMEAKLALS 480 LILRHFALELSPLYAHAPSVTITLQPQYGAHIILHKR 517 SEQIDNO:102 Prunusmume MEASRPSCVALSVVLVSIVIAWAWRVLNWVWLRPNKLERCLREQGLTGNSYRLLFGDTKE 60 ISMMVEQAQSKPIKLSTTHDIAPRVIPFSHQIVYTYGRNSFVWMGPTPRVTIMNPEDLKD 120 AFNKSDEFQRAISNPIVKSISQGLSSLEGEKWAKHRKIINPAFHLEKLKGMLPTFYQSCS 180 EMINKWESLVFKEGSREMDVWPYLENLTSDVISRAAFGSSYEEGRKIFQLLREEAKFYTI 240 AARSVYIPGWRFLPTKQNKRMKEIHKEVRGLLKGIINKREDAIKAGEAAKGNLLGILMES 300 NFREIQEHGNNKNAGMSIEDVIGECKLFYFAGQETTSVLLVWTLVLLSQNQDWQARAREE 360 VLQVFGTNIPTYDQLSHLKVVTMILLEVLRLYPAVVELPRTTYKKTQLGKFLLPAGVEVS 420 LHIMLAHHDKELWGEDAKEFKPERFSEGVSKATKNQFTYFPFGAGPRICIGQNFAMLEAK 480 LALSLILQHFTFELSPSYAHAPSVTITLHPQFGAHFILHKR 521 SEQIDNO:103 Prunusmume CVALSVVLVSIVIAWAWRVLNWVWLRPNKLERCLREQGLTGNSYRLLFGDTKEISMMVEQ 60 AQSKPIKLSTTHDIAPRVIPFSHQIVYTYGRNSFVWMGPTPRVTIMNPEDLKDAFNKSDE 120 FQRAISNPIVKSISQGLSSLEGEKWAKHRKIINPAFHLEKLKGMLPTFYQSCSEMINKWE 180 SLVFKEGSREMDVWPYLENLTSDVISRAAFGSSYEEGRKIFQLLREEAKFYTIAARSVYI 240 PGWRFLPTKQNKRMKEIHKEVRGLLKGIINKREDAIKAGEAAKGNLLGILMESNFREIQE 300 HGNNKNAGMSIEDVIGECKLFYFAGQETTSVLLVWTLVLLSQNQDWQARAREEVLQVFGT 360 NIPTYDQLSHLKVVTMILLEVLRLYPAVVELPRTTYKKTQLGKFLLPAGVEVSLHIMLAH 420 HDKELWGEDAKEFKPERFSEGVSKATKNQFTYFPFGAGPRICIGQNFAMLEAKLALSLIL 480 QHFTFELSPSYAHAPSVTITLHPQFGAHFILHKR 514 SEQIDNO:104 Prunuspersica MGPIPRVHIMNPEDLKDTFNRHDDFHKVVKNPIMKSLPQGIVGIEGDQWAKHRKIINPAF 60 HLEKLKGMVPIFYQSCSEMINIWKSLVSKESSCELDVWPYLENFTSDVISRAAFGSSYEE 120 GRKIFQLLREEAKVYTVAVRSVYIPGWRFLPTKQNKKTKEIHNEIKGLLKGIINKREEAM 180 KAGEATKDDLLGILMESNFREIQEHGNNKNAGMSIEDVIGECKLFYFAGQETTSVLLVWT 240 MVLLSQNQDWQARAREEVLQVFGSNIPTYEELSHLKVVTMILLEVLRLYPSVVALPRTTH 300 KKTQLGKLSLPAGVEVSLPILLVHHDKELWGEDANEFKPERFSEGVSKATKNQFTYFPFG 360 GGPRICIGQNFAMMEAKLALSLILQHFTFELSPQYSHAPSVTITLQPQYGAHLILHKR 418 SEQIDNO:105 ArtificialSequence atgggtttgttcccattagaggattcctacgcgctggtctttgaaggactagcaataaca 60 ctggctttgtactatctactgtctttcatctacaaaacatctaaaaagacatgtacacct 120 cctaaagcatctggtgaaatcattccaattacaggaatcatattgaatctgctatctggc 180 tcaagtggtctacctattatcttagcacttgcctctttagcagacagatgtggtcctatt 240 ttcaccattaggctgggtattaggagagtgctagtagtatcaaattgggaaatcgctaag 300 gagattttcactacccacgatttgatagtttctaatagaccaaaatacttagccgctaag 360 attcttggtttcaattatgtttcattctctttcgctccatacggcccatattgggtcgga 420 atcagaaagattattgctacaaaactaatgtcttcttccagacttcagaagttgcaattt 480 gtaagagtttttgaactagaaaactctatgaaatctatcagagaatcatggaaggagaaa 540 aaggatgaagagggaaaggtattagttgagatgaaaaagtggttctgggaactgaatatg 600 aacatagtgttaaggacagttgctggtaaacaatacactggtacagttgatgatgccgat 660 gcaaagcgtatctccgagttattcagagaatggtttcactacactggcagatttgtcgtt 720 ggagacgcttttccttttctaggttggttggacctgggcggatacaaaaagacaatggaa 780 ttagttgctagtagattggactcaatggtcagtaaatggttagatgagcatcgtaaaaag 840 caagctaacgatgacaaaaaggaggatatggatttcatggatatcatgatctccatgaca 900 gaagcaaattcaccacttgaaggatacggcactgatactattatcaagaccacatgtatg 960 actttgattgtttcaggagttgatacaacctcaatcgtacttacttgggccttatcactt 1020 ttgttaaacaacagagatactttgaaaaaggcacaagaggaattagatatgtgcgtaggt 1080 aaaggaagacaagtcaacgagtctgatcttgttaacttgatatacttggaagcagtgctt 1140 aaagaggctttaagactttacccagcagcgttcttaggcggaccaagagcattcttggaa 1200 gattgtactgttgctggttatagaattccaaagggcacctgcttgttgattaacatgtgg 1260 aaactgcatagagatccaaacatttggagtgatccttgcgaattcaagccagaaagattt 1320 ttgacacctaatcaaaaggatgttgatgtgatcggtatggatttcgaattgataccattt 1380 ggtgccggcagaagatattgtccaggtactagattggctttacagatgttgcatatcgta 1440 ttagcgacattgctgcaaaacttcgaaatgtcaacaccaaacgatgcgccagtcgatatg 1500 actgcttctgttggcatgacaaatgccaaagcatcacctttagaagtcttgctatcacct 1560 cgtgttaaatggtcctaa 1578 SEQIDNO:106 Steviarebaudiana MGLFPLEDSYALVFEGLAITLALYYLLSFIYKTSKKTCTPPKASGEHPITGHLNLLSGSS 60 GLPHLALASLADRCGPIFTIRLGIRRVLVVSNWEIAKEIFTTHDLIVSNRPKYLAAKILG 120 FNYVSFSFAPYGPYWVGIRKIIATKLMSSSRLQKLQFVRVFELENSMKSIRESWKEKKDE 180 EGKVLVEMKKWFWELNMNIVLRTVAGKQYTGTVDDADAKRISELFREWFHYTGRFVVGDA 240 FPFLGWLDLGGYKKTMELVASRLDSMVSKWLDEHRKKQANDDKKEDMDFMDIMISMTEAN 300 SPLEGYGTDTIIKTTCMTLIVSGVDTTSIVLTWALSLLLNNRDTLKKAQEELDMCVGKGR 360 QVNESDLVNLIYLEAVLKEALRLYPAAFLGGPRAFLEDCTVAGYRIPKGTCLLINMWKLH 420 RDPNIWSDPCEFKPERFLTPNQKDVDVIGMDFELIPFGAGRRYCPGTRLALQMLHIVLAT 480 LLQNFEMSTPNDAPVDMTASVGMTNAKASPLEVLLSPRVKWS 522 SEQIDNO:107 ArtificialSequence atgatacaagttttaactccaattctactcttcctcatcttcttcgttttctggaaagtc 60 tacaaacatcaaaagactaaaatcaatctaccaccaggttccttcggctggccatttttg 120 ggtgaaaccttagccttacttagagcaggctgggattctgagccagaaagattcgtaaga 180 gagcgtatcaaaaagcatggatctccacttgttttcaagacatcactatttggagacaga 240 ttcgctgttctttgcggtccagctggtaataagtttttgttctgcaacgaaaacaaatta 300 gtggcatcttggtggccagtccctgtaaggaagttgttcggtaaaagtttactcacaata 360 agaggagatgaagcaaaatggatgagaaaaatgctattgtcttacttgggtccagatgca 420 tttgccacacattatgccgttactatggatgttgtaacacgtagacatattgatgtccat 480 tggaggggcaaggaggaagttaatgtatttcaaacagttaagttgtacgcattcgaatta 540 gcttgtagattattcatgaacctagatgacccaaaccacatcgcgaaactcggtagtctt 600 ttcaacattttcctcaaagggatcatcgagcttcctatagacgttcctggaactagattt 660 tactccagtaaaaaggccgcagctgccattagaattgaattgaaaaagctcattaaagct 720 agaaaactcgaattgaaggagggtaaggcgtcttcttcacaggacttgctttctcatcta 780 ttaacatcacctgatgagaatgggatgttcttgacagaagaggaaatagtcgataacatt 840 ctacttttgttattcgctggtcacgatacctctgcactatcaataacacttttgatgaaa 900 accttaggtgaacacagtgatgtgtacgacaaggttttgaaggaacaattagaaatttcc 960 aaaacaaaggaggcttgggaatcactaaagtgggaagatatccagaagatgaagtactca 1020 tggtcagtaatctgtgaagtcatgagattgaatcctcctgtcatagggacatacagagag 1080 gcgttggttgatatcgactatgctggttacactatcccaaaaggatggaagttgcattgg 1140 tcagctgtttctactcaaagagacgaagccaatttcgaagatgtaactagattcgatcca 1200 tccagatttgaaggggcaggccctactccattcacatttgtgcctttcggtggaggtcct 1260 agaatgtgtttaggcaaagagtttgccaggttagaagtgttagcatttctccacaacatt 1320 gttaccaactttaagtgggatcttctaatccctgatgagaagatcgaatatgatccaatg 1380 gctactccagctaagggcttgccaattagacttcatccacaccaagtctaa 1431 SEQIDNO:108 Steviarebaudiana MIQVLTPILLFLIFFVFWKVYKHQKTKINLPPGSFGWPFLGETLALLRAGWDSEPERFVR 60 ERIKKHGSPLVFKTSLFGDRFAVLCGPAGNKFLFCNENKLVASWWPVPVRKLFGKSLLTI 120 RGDEAKWMRKMLLSYLGPDAFATHYAVTMDVVTRRHIDVHWRGKEEVNVFQTVKLYAFEL 180 ACRLFMNLDDPNHIAKLGSLFNIFLKGIIELPIDVPGTRFYSSKKAAAAIRIELKKLIKA 240 RKLELKEGKASSSQDLLSHLLTSPDENGMFLTEEEIVDNILLLLFAGHDTSALSITLLMK 300 TLGEHSDVYDKVLKEQLEISKTKEAWESLKWEDIQKMKYSWSVICEVMRLNPPVIGTYRE 360 ALVDIDYAGYTIPKGWKLHWSAVSTQRDEANFEDVTRFDPSRFEGAGPTPFTFVPFGGGP 420 RMCLGKEFARLEVLAFLHNIVTNFKWDLLIPDEKIEYDPMATPAKGLPIRLHPHQV 476 SEQIDNO:109 ArtificialSequence atggagtctttagtggttcatacagtaaatgctatctggtgtattgtaatcgtcgggatt 60 ttctcagttggttatcacgtttacggtagagctgtggtcgaacaatggagaatgagaaga 120 tcactgaagctacaaggtgttaaaggcccaccaccatccatcttcaatggtaacgtctca 180 gaaatgcaacgtatccaatccgaagctaaacactgctctggcgataacattatctcacat 240 gattattcttcttcattattcccacacttcgatcactggagaaaacagtacggcagaatc 300 tacacatactctactggattaaagcaacacttgtacatcaatcatccagaaatggtgaag 360 gagctatctcagactaacacattgaacttgggtagaatcacccatataaccaaaagattg 420 aatcctatcttaggtaacggaatcataacctctaatggtcctcattgggcccatcagcgt 480 agaattatcgcctacgagtttactcatgataagatcaagggtatggttggtttgatggtt 540 gagtctgctatgcctatgttgaataagtgggaggagatggtaaagagaggcggagaaatg 600 ggatgcgacataagagttgatgaggacttgaaagatgtttcagcagatgtgattgcaaaa 660 gcctgtttcggatcctcattttctaaaggtaaggctattttctctatgataagagatttg 720 cttacagctatcacaaagagaagtgttctattcagattcaacggattcactgatatggtc 780 tttgggagtaaaaagcatggtgacgttgatatagacgctttagaaatggaattggaatca 840 tccatttgggaaactgtcaaggaacgtgaaatagaatgtaaagatactcacaaaaaggat 900 ctgatgcaattgattttggaaggggcaatgcgttcatgtgacggtaacctttgggataaa 960 tcagcatatagaagatttgttgtagataattgtaaatctatctacttcgcagggcatgat 1020 agtacagctgtctcagtgtcatggtgtttgatgttactggccctaaacccatcatggcaa 1080 gttaagatccgtgatgaaattctgtcttcttgcaaaaatggtattccagatgccgaaagt 1140 atcccaaaccttaaaacagtgactatggttattcaagagacaatgagattataccctcca 1200 gcaccaatcgtcgggagagaagcctctaaagatatcagattgggcgatctagttgttcct 1260 aaaggcgtctgtatatggacactaataccagctttacacagagatcctgagatttgggga 1320 ccagatgcaaacgatttcaaaccagaaagattttctgaaggaatttcaaaggcttgtaag 1380 tatcctcaaagttacattccatttggtctgggtcctagaacatgcgttggtaaaaacttt 1440 ggcatgatggaagtaaaggttcttgtttccctgattgtctccaagttctctttcactcta 1500 tctcctacctaccaacatagtcctagtcacaaacttttagtagaaccacaacatggggtg 1560 gtaattagagtggtttaa 1578 SEQIDNO:110 Arabidopsisthaliana MESLVVHTVNAIWCIVIVGIFSVGYHVYGRAVVEQWRMRRSLKLQGVKGPPPSIFNGNVS 60 EMQRIQSEAKHCSGDNIISHDYSSSLFPHFDHWRKQYGRIYTYSTGLKQHLYINHPEMVK 120 ELSQTNTLNLGRITHITKRLNPILGNGIITSNGPHWAHQRRIIAYEFTHDKIKGMVGLMV 180 ESAMPMLNKWEEMVKRGGEMGCDIRVDEDLKDVSADVIAKACFGSSFSKGKAIFSMIRDL 240 LTAITKRSVLFRFNGFTDMVFGSKKHGDVDIDALEMELESSIWETVKEREIECKDTHKKD 300 LMQLILEGAMRSCDGNLWDKSAYRRFVVDNCKSIYFAGHDSTAVSVSWCLMLLALNPSWQ 360 VKIRDEILSSCKNGIPDAESIPNLKTVTMVIQETMRLYPPAPIVGREASKDIRLGDLVVP 420 KGVCIWTLIPALHRDPEIWGPDANDFKPERFSEGISKACKYPQSYIPFGLGPRTCVGKNF 480 GMMEVKVLVSLIVSKFSFTLSPTYQHSPSHKLLVEPQHGVVIRVV 525 SEQIDNO:111 ArtificialSequence atgtacttcctactacaatacctcaacatcacaaccgttggtgtctttgccacattgttt 60 ctctcttattgtttacttctctggagaagtagagcgggtaacaaaaagattgccccagaa 120 gctgccgctgcatggcctattatcggccacctccacttacttgcaggtggatcccatcaa 180 ctaccacatattacattgggtaacatggcagataagtacggtcctgtattcacaatcaga 240 ataggcttgcatagagctgtagttgtctcatcttgggaaatggcaaaggaatgttcaaca 300 gctaatgatcaagtgtcttcttcaagacctgaactattagcttctaagttgttgggttat 360 aactacgccatgtttggtttttcaccatacggttcatactggagagaaatgagaaagatc 420 atctctctcgaattactatctaattccagattggaactattgaaagatgttagagcctca 480 gaagttgtcacatctattaaggaactatacaaattgtgggcggaaaagaagaatgagtca 540 ggattggtttctgtcgagatgaaacaatggttcggagatttgactttaaacgtgatcttg 600 agaatggtggctggtaaaagatacttctccgcgagtgacgcttcagaaaacaaacaggcc 660 cagcgttgtagaagagtcttcagagaattcttccatctctccggcttgtttgtggttgct 720 gatgctataccttttcttggatggctcgattggggaagacacgagaagaccttgaaaaag 780 accgccatagaaatggattccatcgcccaggagtggcttgaggaacatagacgtagaaaa 840 gattctggagatgataattctacccaagatttcatggacgttatgcaatctgtgctagat 900 ggcaaaaatctaggcggatacgatgctgatacgattaacaaggctacatgcttaactctt 960 atatcaggtggcagtgatactactgtagtttctttgacatgggctcttagtcttgtgtta 1020 aacaatagagatactttgaaaaaggcacaggaagagttagacatccaagtcggtaaggaa 1080 agattggttaacgagcaagacatcagtaagttagtttacttgcaagcaatagtaaaagag 1140 acactcagactttatccaccaggtcctttgggtggtttgagacaattcactgaagattgt 1200 acactaggtggctatcacgtttcaaaaggaactagattaatcatgaacttatccaagatt 1260 caaaaagatccacgtatttggtctgatcctactgaattccaaccagagagattccttacg 1320 actcataaagatgtcgatccacgtggtaaacactttgaattcattccattcggtgcagga 1380 agacgtgcatgtcctggtatcacattcggattacaagtactacatctaacattggcatct 1440 ttcttgcatgcgtttgaattttcaacaccatcaaatgagcaggttaacatgagagaatca 1500 ttaggtcttacgaatatgaaatctaccccattagaagttttgatttctccaagactatcc 1560 cttaattgcttcaaccttatgaaaatttga 1590 SEQIDNO:112 Vitisvinifera MYFLLQYLNITTVGVFATLFLSYCLLLWRSRAGNKKIAPEAAAAWPIIGHLHLLAGGSHQ 60 LPHITLGNMADKYGPVFTIRIGLHRAVVVSSWEMAKECSTANDQVSSSRPELLASKLLGY 120 NYAMFGFSPYGSYWREMRKIISLELLSNSRLELLKDVRASEVVTSIKELYKLWAEKKNES 180 GLVSVEMKQWFGDLTLNVILRMVAGKRYFSASDASENKQAQRCRRVFREFFHLSGLFVVA 240 DAIPFLGWLDWGRHEKTLKKTAIEMDSIAQEWLEEHRRRKDSGDDNSTQDFMDVMQSVLD 300 GKNLGGYDADTINKATCLTLISGGSDTTVVSLTWALSLVLNNRDTLKKAQEELDIQVGKE 360 RLVNEQDISKLVYLQAIVKETLRLYPPGPLGGLRQFTEDCTLGGYHVSKGTRLIMNLSKI 420 QKDPRIWSDPTEFQPERFLTTHKDVDPRGKHFEFIPFGAGRRACPGITFGLQVLHLTLAS 480 FLHAFEFSTPSNEQVNMRESLGLTNMKSTPLEVLISPRLSSCSLYN 526 SEQIDNO:113 ArtificialSequence atggaacctaacttttacttgtcattactattgttgttcgtgaccttcatttctttaagt 60 ctgtttttcatcttttacaaacaaaagtccccattgaatttgccaccagggaaaatgggt 120 taccctatcataggtgaaagtttagaattcctatccacaggctggaagggacatcctgaa 180 aagttcatatttgatagaatgcgtaagtacagtagtgagttattcaagacttctattgta 240 ggcgaatccacagttgtttgctgtggggcagctagtaacaaattcctattctctaacgaa 300 aacaaactggtaactgcctggtggccagattctgttaacaaaatcttcccaacaacttca 360 ctggattctaatttgaaggaggaatctataaagatgagaaagttgctgccacagttcttc 420 aaaccagaagcacttcaaagatacgtcggcgttatggatgtaatcgcacaaagacatttt 480 gtcactcactgggacaacaaaaatgagatcacagtttatccacttgctaaaagatacact 540 ttcttgcttgcgtgtagactgttcatgtctgttgaggatgaaaatcatgtggcgaaattc 600 tcagacccattccaactaatcgctgcaggcatcatttcacttcctatcgatcttcctggt 660 actccattcaacaaggccataaaggcttcaaatttcattagaaaagagctgataaagatt 720 atcaaacaaagacgtgttgatctggcagagggtacagcatctccaacccaggatatcttg 780 tcacatatgctattaacatctgatgaaaacggtaaatctatgaacgagttgaacattgcc 840 gacaagattcttggactattgataggaggccacgatacagcttcagtagcttgcacattt 900 ctagtgaagtacttaggagaattaccacatatctacgataaagtctaccaagagcaaatg 960 gaaattgccaagtccaaacctgctggggaattgttgaattgggatgacttgaaaaagatg 1020 aagtattcatggaatgtggcatgtgaggtaatgagattgtcaccacctttacaaggtggt 1080 tttagagaggctataactgactttatgtttaacggtttctctattccaaaagggtggaag 1140 ttatactggtccgccaactctacacacaaaaatgcagaatgtttcccaatgcctgagaaa 1200 ttcgatcctaccagatttgaaggtaatggtccagcgccttatacatttgtaccattcggt 1260 ggaggccctagaatgtgtcctggaaaggaatacgctagattagaaatcttggttttcatg 1320 cataatctggtcaaacgttttaagtgggaaaaggttattccagacgaaaagattattgtc 1380 gatccattcccaatcccagctaaagatcttccaatccgtttgtatcctcacaaagcttaa 1440 SEQIDNO:114 Medicagotruncatula MEPNFYLSLLLLFVTFISLSLFFIFYKQKSPLNLPPGKMGYPIIGESLEFLSTGWKGHPE 60 KFIFDRMRKYSSELFKTSIVGESTVVCCGAASNKFLFSNENKLVTAWWPDSVNKIFPTTS 120 LDSNLKEESIKMRKLLPQFFKPEALQRYVGVMDVIAQRHFVTHWDNKNEITVYPLAKRYT 180 FLLACRLFMSVEDENHVAKFSDPFQLIAAGIISLPIDLPGTPFNKAIKASNFIRKELIKI 240 IKQRRVDLAEGTASPTQDILSHMLLTSDENGKSMNELNIADKILGLLIGGHDTASVACTF 300 LVKYLGELPHIYDKVYQEQMEIAKSKPAGELLNWDDLKKMKYSWNVACEVMRLSPPLQGG 360 FREAITDFMFNGFSIPKGWKLYWSANSTHKNAECFPMPEKFDPTRFEGNGPAPYTFVPFG 420 GGPRMCPGKEYARLEILVFMHNLVKRFKWEKVIPDEKIIVDPFPIPAKDLPIRLYPHKA 479 SEQIDNO:115 ArtificialSequence atggcctctgttactttgggttcctggatcgtcgtccaccaccataaccatcaccatcca 60 tcatctatcctaactaaatctcgttcaagatcctgtcctattacactaaccaaaccaatc 120 tcttttcgttcaaagagaacagtttcctctagtagttctatcgtgtcctctagtgtcgtc 180 actaaggaagacaatctgagacagtctgaaccttcttcctttgatttcatgtcatatatc 240 attactaaggcagaactagtgaataaggctcttgattcagcagttccattaagagagcca 300 ttgaaaatccatgaagcaatgagatactctcttctagctggcgggaagagagtcagacct 360 gtactctgcatagcagcgtgcgaattagttggtggcgaggaatcaaccgctatgcctgcc 420 gcttgtgctgtagaaatgattcatacaatgtcactgatacacgatgatttgccatgtatg 480 gataacgatgatctgagaaggggtaagccaactaaccataaggttttcggcgaagatgtt 540 gccgtcttagctggtgatgctttgttatctttcgcgttcgaacatttggcatccgcaaca 600 tcaagtgatgttgtgtcaccagtaagagtagttagagcagttggagaactggctaaagct 660 attggaactgagggtttagttgcaggtcaagtcgtcgatatctcttccgaaggtcttgat 720 ttgaatgatgtaggtcttgaacatctcgaattcatccatcttcacaagacagctgcactt 780 ttagaagccagtgcggttctcggcgcaattgttggcggagggagtgatgacgaaattgag 840 agattgaggaagtttgctagatgtataggattactgttccaagtagtagacgatatacta 900 gatgtgacaaagtcttccaaagagttgggaaaaacagctggtaaagatttgattgccgac 960 aaattgacctaccctaagattatggggctagaaaaatcaagagaatttgccgagaaactc 1020 aatagagaggcgcgtgatcaactgttgggtttcgattctgataaagttgcaccactctta 1080 gccttagccaactacatcgcttacagacaaaactaa 1116 SEQIDNO:116 Arabidopsisthaliana MASVTLGSWIVVHHHNHHHPSSILTKSRSRSCPITLTKPISFRSKRTVSSSSSIVSSSVV 60 TKEDNLRQSEPSSFDFMSYIITKAELVNKALDSAVPLREPLKIHEAMRYSLLAGGKRVRP 120 VLCIAACELVGGEESTAMPAACAVEMIHTMSLIHDDLPCMDNDDLRRGKPTNHKVFGEDV 180 AVLAGDALLSFAFEHLASATSSDVVSPVRVVRAVGELAKAIGTEGLVAGQVVDISSEGLD 240 LNDVGLEHLEFIHLHKTAALLEASAVLGAIVGGGSDDEIERLRKFARCIGLLFQVVDDIL 300 DVTKSSKELGKTAGKDLIADKLTYPKIMGLEKSREFAEKLNREARDQLLGFDSDKVAPLL 360 ALANYIAYRQN 371 SEQIDNO:117 Rubussuavissimus MATLLEHFQAMPFAIPIALAALSWLFLFYIKVSFFSNKSAQAKLPPVPVVPGLPVIGNLL 60 QLKEKKPYQTFTRWAEEYGPIYSIRTGASTMVVLNTTQVAKEAMVTRYLSISTRKLSNAL 120 KILTADKCMVAISDYNDFHKMIKRYILSNVLGPSAQKRHRSNRDTLRANVCSRLHSQVKN 180 SPREAVNFRRVFEWELFGIALKQAFGKDIEKPIYVEELGTTLSRDEIFKVLVLDIMEGAI 240 EVDWRDFFPYLRWIPNTRMETKIQRLYFRRKAVMTALINEQKKRIASGEEINCYIDFLLK 300 EGKTLTMDQISMLLWETVIETADTTMVTTEWAMYEVAKDSKRQDRLYQEIQKVCGSEMVT 360 EEYLSQLPYLNAVFHETLRKHSPAALVPLRYAHEDTQLGGYYIPAGTEIAINIYGCNMDK 420 HQWESPEEWKPERFLDPKFDPMDLYKTMAFGAGKRVCAGSLQAMLIACPTIGRLVQEFEW 480 KLRDGEEENVDTVGLTTHKRYPMHAILKPRS 511 SEQIDNO:126 Arabidopsisthaliana atggcatcggaatttcgtcctcctcttcattttgttctcttccctttcatggctcaaggc 60 cacatgatcccaatggtagatattgcaaggctcctggctcagcgcggggtgactataacc 120 attgtcactacacctcaaaacgcaggccggttcaagaacgttcttagccgggctatccaa 180 tccggcttgcccatcaatctcgtgcaagtaaagtttccatctcaagaatcgggttcaccg 240 gaaggacaggagaatttggacttgctcgattcattgggggcttcattaaccttcttcaaa 300 gcatttagcctgctcgaggaaccagtcgagaagctcttgaaagagattcaacctaggcca 360 aactgcataatcgctgacatgtgtttgccttatacaaacagaattgccaagaatcttggt 420 ataccaaaaatcatctttcatggcatgtgttgcttcaatcttctttgtacgcacataatg 480 caccaaaaccacgagttcttggaaactatagagtctgacaaggaatacttccccattcct 540 aatttccctgacagagttgagttcacaaaatctcagcttccaatggtattagttgctgga 600 gattggaaagacttccttgacggaatgacagaaggggataacacttcttatggtgtgatt 660 gttaacacgtttgaagagctcgagccagcttatgttagagactacaagaaggttaaagcg 720 ggtaagatatggagcatcggaccggtttccttgtgcaacaagttaggagaagaccaagct 780 gagaggggaaacaaggcggacattgatcaagacgagtgtattaaatggcttgattctaaa 840 gaagaagggtcggtgctatatgtttgccttggaagtatatgcaatcttcctctgtctcag 900 ctcaaagagctcggcttaggcctcgaggaatcccaaagacctttcatttgggtcataaga 960 ggttgggagaagtataacgagttacttgaatggatctcagagagcggttataaggaaaga 1020 atcaaagaaagaggccttctcataacaggatggtcgcctcaaatgcttatccttacacat 1080 cctgccgttggaggattcttgacacattgtggatggaactctactcttgaaggaatcact 1140 tcaggcgttccattactcacgtggccactgtttggagaccaattctgcaatgagaaattg 1200 gcggtgcagatactaaaagccggtgtgagagctggggttgaagagtccatgagatgggga 1260 gaagaggagaaaataggagtactggtggataaagaaggagtaaagaaggcagtggaggaa 1320 ttgatgggtgatagtaatgatgctaaggagagaagaaaaagagtgaaagagcttggagaa 1380 ttagctcacaaggctgtggaagaaggaggctcttctcattccaacatcacattcttgcta 1440 caagacataatgcaattagaacaacccaagcgctag 1476 SEQIDNO:127 Arabidopsisthaliana MASEFRPPLHFVLFPFMAQGHMIPMVDIARLLAQRGVTITIVTTPQNAGRFKNVLSRAIQ 60 SGLPINLVQVKFPSQESGSPEGQENLDLLDSLGASLTFFKAFSLLEEPVEKLLKEIQPRP 120 NCIIADMCLPYTNRIAKNLGIPKIIFHGMCCFNLLCTHIMHQNHEFLETIESDKEYFPIP 180 NFPDRVEFTKSQLPMVLVAGDWKDFLDGMTEGDNTSYGVIVNTFEELEPAYVRDYKKVKA 240 GKIWSIGPVSLCNKLGEDQAERGNKADIDQDECIKWLDSKEEGSVLYVCLGSICNLPLSQ 300 LKELGLGLEESQRPFIWVIRGWEKYNELLEWISESGYKERIKERGLLITGWSPQMLILTH 360 PAVGGFLTHCGWNSTLEGITSGVPLLTWPLFGDQFCNEKLAVQILKAGVRAGVEESMRWG 420 EEEKIGVLVDKEGVKKAVEELMGDSNDAKERRKRVKELGELAHKAVEEGGSSHSNITFLL 480 QDIMQLEQPKR 491 SEQIDNO:132 Arabidopsisthaliana atggctacggaaaaaacccaccaatttcatccttctcttcactttgtcctcttccctttc 60 atggctcaaggccacatgattcccatgattgatattgcaagactcttggctcagcgtggt 120 gtgaccataacaattgtcacgacacctcacaacgcagcaaggtttaagaatgtcctaaac 180 cgagcgatcgagtctggcttggccatcaacatactgcatgtgaagtttccatatcaagag 240 tttggtttgccagaaggaaaagagaatatagattcgttagactcaacggagttgatggta 300 cctttcttcaaagcggtgaacttgcttgaagatccggtcatgaagctcatggaagagatg 360 aaacctagacctagctgtctaatttctgattggtgtttgccttatacaagcataatcgcc 420 aagaacttcaatataccaaagatagttttccacggcatgggttgctttaatcttttgtgt 480 atgcatgttctacgcagaaacttagagatcctagagaatgtaaagtcggatgaagagtat 540 ttcttggttcctagttttcctgatagagttgaatttacaaagcttcaacttcctgtgaaa 600 gcaaatgcaagtggagattggaaagagataatggatgaaatggtaaaagcagaatacaca 660 tcctatggtgtgatcgtcaacacatttcaggagttggagccaccttatgtcaaagactac 720 aaagaggcaatggatggaaaagtatggtccattggacccgtttccttgtgtaacaaggca 780 ggtgcagacaaagctgagaggggaagcaaggccgccattgatcaagatgagtgtcttcaa 840 tggcttgattctaaagaagaaggttcggtgctctatgtttgccttggaagtatatgtaat 900 cttcctttgtctcagctcaaggagctggggctaggccttgaggaatctcgaagatctttt 960 atttgggtcataagaggttcggaaaagtataaagaactatttgagtggatgttggagagc 1020 ggttttgaagaaagaatcaaagagagaggacttctcattaaagggtgggcacctcaagtc 1080 cttatcctttcacatccttccgttggaggattcctgacacactgtggatggaactcgact 1140 ctcgaaggaatcacctcaggcattccactgatcacttggccgctgtttggagaccaattc 1200 tgcaaccaaaaactggtcgttcaagtactaaaagccggtgtaagtgccggggttgaagaa 1260 gtcatgaaatggggagaagaagataaaataggagtgttagtggataaagaaggagtgaaa 1320 aaggctgtggaagaattgatgggtgatagtgatgatgcaaaagagaggagaagaagagtc 1380 aaagagcttggagaattagctcacaaagctgtggaaaaaggaggctcttctcattctaac 1440 atcacactcttgctacaagacataatgcaactagcacaattcaagaattga 1491 SEQIDNO:133 Arabidopsisthaliana MATEKTHQFHPSLHFVLFPFMAQGHMIPMIDIARLLAQRGVTITIVTTPHNAARFKNVLN 60 RAIESGLAINILHVKFPYQEFGLPEGKENIDSLDSTELMVPFFKAVNLLEDPVMKLMEEM 120 KPRPSCLISDWCLPYTSIIAKNFNIPKIVFHGMGCFNLLCMHVLRRNLEILENVKSDEEY 180 FLVPSFPDRVEFTKLQLPVKANASGDWKEIMDEMVKAEYTSYGVIVNTFQELEPPYVKDY 240 KEAMDGKVWSIGPVSLCNKAGADKAERGSKAAIDQDECLQWLDSKEEGSVLYVCLGSICN 300 LPLSQLKELGLGLEESRRSFIWVIRGSEKYKELFEWMLESGFEERIKERGLLIKGWAPQV 360 LILSHPSVGGFLTHCGWNSTLEGITSGIPLITWPLFGDQFCNQKLVVQVLKAGVSAGVEE 420 VMKWGEEDKIGVLVDKEGVKKAVEELMGDSDDAKERRRRVKELGELAHKAVEKGGSSHSN 480 ITLLLQDIMQLAQFKN 496 SEQIDNO:134 Arabidopsisthaliana atggtttccgaaacaaccaaatcttctccacttcactttgttctcttccctttcatggct 60 caaggccacatgattcccatggttgatattgcaaggctcttggctcagcgtggtgtgatc 120 ataacaattgtcacgacgcctcacaatgcagcgaggttcaagaatgtcctaaaccgtgcc 180 attgagtctggcttgcccatcaacttagtgcaagtcaagtttccatatctagaagctggt 240 ttgcaagaaggacaagagaatatcgattctcttgacacaatggagcggatgatacctttc 300 tttaaagcggttaactttctcgaagaaccagtccagaagctcattgaagagatgaaccct 360 cgaccaagctgtctaatttctgatttttgtttgccttatacaagcaaaatcgccaagaag 420 ttcaatatcccaaagatcctcttccatggcatgggttgcttttgtcttctgtgtatgcat 480 gttttacgcaagaaccgtgagatcttggacaatttaaagtcagataaggagcttttcact 540 gttcctgattttcctgatagagttgaattcacaagaacgcaagttccggtagaaacatat 600 gttccagctggagactggaaagatatctttgatggtatggtagaagcgaatgagacatct 660 tatggtgtgatcgtcaactcatttcaagagctcgagcctgcttatgccaaagactacaag 720 gaggtaaggtccggtaaagcatggaccattggacccgtttccttgtgcaacaaggtagga 780 gccgacaaagcagagaggggaaacaaatcagacattgatcaagatgagtgccttaaatgg 840 ctcgattctaagaaacatggctcggtgctttacgtttgtcttggaagtatctgtaatctt 900 cctttgtctcaactcaaggagctgggactaggcctagaggaatcccaaagacctttcatt 960 tgggtcataagaggttgggagaagtacaaagagttagttgagtggttctcggaaagcggc 1020 tttgaagatagaatccaagatagaggacttctcatcaaaggatggtcccctcaaatgctt 1080 atcctttcacatccatcagttggagggttcctaacacactgtggttggaactcgactctt 1140 gaggggataactgctggtctaccgctacttacatggccgctattcgcagaccaattctgc 1200 aatgagaaattggtcgttgaggtactaaaagccggtgtaagatccggggttgaacagcct 1260 atgaaatggggagaagaggagaaaataggagtgttggtggataaagaaggagtgaagaag 1320 gcagtggaagaattaatgggtgagagtgatgatgcaaaagagagaagaagaagagccaaa 1380 gagcttggagattcagctcacaaggctgtggaagaaggaggctcttctcattctaacatc 1440 tctttcttgctacaagacataatggaactggcagaacccaataattga 1488 SEQIDNO:135 Arabidopsisthaliana MVSETTKSSPLHFVLFPFMAQGHMIPMVDIARLLAQRGVIITIVTTPHNAARFKNVLNRA 60 IESGLPINLVQVKFPYLEAGLQEGQENIDSLDTMERMIPFFKAVNFLEEPVQKLIEEMNP 120 RPSCLISDFCLPYTSKIAKKFNIPKILFHGMGCFCLLCMHVLRKNREILDNLKSDKELFT 180 VPDFPDRVEFTRTQVPVETYVPAGDWKDIFDGMVEANETSYGVIVNSFQELEPAYAKDYK 240 EVRSGKAWTIGPVSLCNKVGADKAERGNKSDIDQDECLKWLDSKKHGSVLYVCLGSICNL 300 PLSQLKELGLGLEESQRPFIWVIRGWEKYKELVEWFSESGFEDRIQDRGLLIKGWSPQML 360 ILSHPSVGGFLTHCGWNSTLEGITAGLPLLTWPLFADQFCNEKLVVEVLKAGVRSGVEQP 420 MKWGEEEKIGVLVDKEGVKKAVEELMGESDDAKERRRRAKELGDSAHKAVEEGGSSHSNI 480 SFLLQDIMELAEPNN 495 SEQIDNO:136 Arabidopsisthaliana atggctttcgaaaaaaacaacgaaccttttcctcttcactttgttctcttccctttcatg 60 gctcaaggccacatgattcccatggttgatattgcaaggctcttggctcagcgaggtgtg 120 cttataacaattgtcacgacgcctcacaatgcagcaaggttcaagaatgtcctaaaccgt 180 gccattgagtctggtttgcccatcaacctagtgcaagtcaagtttccatatcaagaagct 240 ggtctgcaagaaggacaagaaaatatggatttgcttaccacgatggagcagataacatct 300 ttctttaaagcggttaacttactcaaagaaccagtccagaaccttattgaagagatgagc 360 ccgcgaccaagctgtctaatctctgatatgtgtttgtcgtatacaagcgaaatcgccaag 420 aagttcaaaataccaaagatcctcttccatggcatgggttgcttttgtcttctgtgtgtt 480 aacgttctgcgcaagaaccgtgagatcttggacaatttaaagtctgataaggagtacttc 540 attgttccttattttcctgatagagttgaattcacaagacctcaagttccggtggaaaca 600 tatgttcctgcaggctggaaagagatcttggaggatatggtagaagcggataagacatct 660 tatggtgttatagtcaactcatttcaagagctcgaacctgcgtatgccaaagacttcaag 720 gaggcaaggtctggtaaagcatggaccattggacctgtttccttgtgcaacaaggtagga 780 gtagacaaagcagagaggggaaacaaatcagatattgatcaagatgagtgccttgaatgg 840 ctcgattctaaggaaccgggatctgtgctctacgtttgccttggaagtatttgtaatctt 900 cctctgtctcagctccttgagctgggactaggcctagaggaatcccaaagacctttcatc 960 tgggtcataagaggttgggagaaatacaaagagttagttgagtggttctcggaaagcggc 1020 tttgaagatagaatccaagatagaggacttctcatcaaaggatggtcccctcaaatgctt 1080 atcctttcacatccttctgttggagggttcttaacgcactgcggatggaactcgactctt 1140 gaggggataactgctggtctaccaatgcttacatggccactatttgcagaccaattctgc 1200 aacgagaaactggtcgtacaaatactaaaagtcggtgtaagtgccgaggttaaagaggtc 1260 atgaaatggggagaagaagagaagataggagtgttggtggataaagaaggagtgaagaag 1320 gcagtggaagaactaatgggtgagagtgatgatgcaaaagagagaagaagaagagccaaa 1380 gagcttggagaatcagctcacaaggctgtggaagaaggaggctcctctcattctaatatc 1440 actttcttgctacaagacataatgcaactagcacagtccaataattga 1488 SEQIDNO:137 Arabidopsisthaliana MAFEKNNEPFPLHFVLFPFMAQGHMIPMVDIARLLAQRGVLITIVTTPHNAARFKNVLNR 60 AIESGLPINLVQVKFPYQEAGLQEGQENMDLLTTMEQITSFFKAVNLLKEPVQNLIEEMS 120 PRPSCLISDMCLSYTSEIAKKFKIPKILFHGMGCFCLLCVNVLRKNREILDNLKSDKEYF 180 IVPYFPDRVEFTRPQVPVETYVPAGWKEILEDMVEADKTSYGVIVNSFQELEPAYAKDFK 240 EARSGKAWTIGPVSLCNKVGVDKAERGNKSDIDQDECLEWLDSKEPGSVLYVCLGSICNL 300 PLSQLLELGLGLEESQRPFIWVIRGWEKYKELVEWFSESGFEDRIQDRGLLIKGWSPQML 360 ILSHPSVGGFLTHCGWNSTLEGITAGLPMLTWPLFADQFCNEKLVVQILKVGVSAEVKEV 420 MKWGEEEKIGVLVDKEGVKKAVEELMGESDDAKERRRRAKELGESAHKAVEEGGSSHSNI 480 TFLLQDIMQLAQSNN 495 SEQIDNO:138 Arabidopsisthaliana atgtgttctcatgatcctcttcacttcgtcgtaataccctttatggcccaaggccatatg 60 atcccattggtcgacatctctaggctcttgtcccagcgccaaggcgtgactgtctgcatc 120 atcacaactactcaaaatgtagccaagatcaagacttcactctcattttcctctttgttt 180 gcgactatcaacatcgttgaagttaagtttctgtctcaacaaacgggtttgccagaaggg 240 tgcgagagtttagatatgttggcttcaatgggcgatatggtgaagttctttgatgctgcc 300 aactcacttgaggagcaagttgagaaagctatggaagagatggttcagccgcggccaagc 360 tgcatcattggagacatgagccttcctttcacttcaagacttgccaagaaattcaagatc 420 cccaaacttatcttccatgggttttcttgtttcagcctcatgtctatacaagtggttcga 480 gaaagcgggatcttgaaaatgatagaatcaaacgacgagtattttgatttgcccggcttg 540 cctgacaaagttgagttcacgaaacctcaggtctctgtgttgcaacctgttgaaggaaat 600 atgaaagagagtacggccaagattattgaagctgataatgactcttatggtgttattgtg 660 aacacttttgaagagttagaggttgattatgcaagagaatataggaaagcaagggctgga 720 aaagtttggtgcgttggacctgtttccttgtgcaataggttagggttagacaaagctaaa 780 agaggagataaggcttctattggtcaagaccaatgtcttcaatggcttgactctcaagaa 840 actggttcagtgctctacgtttgccttggaagtctatgtaatcttcccttggctcagctc 900 aaagagctgggactaggccttgaggcatctaataaacctttcatatgggttataagagaa 960 tggggaaaatatggagatttagcaaattggatgcaacaaagcggatttgaagagcggatc 1020 aaagatagaggactggtgatcaaaggttgggcgccgcaagttttcatcctctcacacgca 1080 tccattggagggtttttgactcactgtggatggaactcgacactagaaggaattactgca 1140 ggagttccattattgacatggcctttgtttgctgaacaattcttgaatgagaagttagtt 1200 gtgcagatactaaaagcagggttaaagataggagtagagaaattgatgaaatatggaaaa 1260 gaagaggagataggagcgatggtgagcagagaatgtgtgagaaaagctgtggatgagcta 1320 atgggtgatagtgaagaagcagaagagagaagaagaaaagttacagaacttagtgacttg 1380 gcaaataaggctttggaaaaaggaggatcttcagattctaatatcacattgctcattcaa 1440 gatattatggagcaatcacaaaatcaattctag 1473 SEQIDNO:139 Arabidopsisthaliana MCSHDPLHFVVIPFMAQGHMIPLVDISRLLSQRQGVTVCIITTTQNVAKIKTSLSFSSLF 60 ATINIVEVKFLSQQTGLPEGCESLDMLASMGDMVKFFDAANSLEEQVEKAMEEMVQPRPS 120 CIIGDMSLPFTSRLAKKFKIPKLIFHGFSCFSLMSIQVVRESGILKMIESNDEYFDLPGL 180 PDKVEFTKPQVSVLQPVEGNMKESTAKIIEADNDSYGVIVNTFEELEVDYAREYRKARAG 240 KVWCVGPVSLCNRLGLDKAKRGDKASIGQDQCLQWLDSQETGSVLYVCLGSLCNLPLAQL 300 KELGLGLEASNKPFIWVIREWGKYGDLANWMQQSGFEERIKDRGLVIKGWAPQVFILSHA 360 SIGGFLTHCGWNSTLEGITAGVPLLTWPLFAEQFLNEKLVVQILKAGLKIGVEKLMKYGK 420 EEEIGAMVSRECVRKAVDELMGDSEEAEERRRKVTELSDLANKALEKGGSSDSNITLLIQ 480 DIMEQSQNQF 490 SEQIDNO:140 Steviarebaudiana atgtcgccaaaaatggtggcaccaccaaccaaccttcattttgttttgtttcctcttatg 60 gctcaaggccatctggtacccatggtcgacatcgctcgaatcttagcccaacgtggtgca 120 acggtcaccataatcaccacaccctaccatgccaaccgggtcagaccggttatctcccga 180 gccatcgcgaccaatctcaagatccagctactcgaactccaactgcggtcaaccgaagcc 240 ggtttacccgaagggtgcgaaagcttcgaccaacttccgtcattcgagtactggaaaaat 300 atttcaaccgctatcgatttgttacaacaacccgctgaagatttgctccgagaactttca 360 ccaccacccgattgcatcatatcggactttttgttcccgtggaccaccgatgtggctcga 420 cggttaaacatcccccggctcgtgttcaatggaccgggctgcttttatctcttgtgcatc 480 catgttgcgatcacttccaacattttgggagagaatgaaccggtcagtagtaataccgag 540 cgcgttgtgctgcccggtttacctgaccggatcgaagtcactaaacttcagatcgtcggt 600 tcgtcgagaccagccaacgtagacgaaatgggctcgtggcttcgagccgtagaagctgag 660 aaagcttcattcgggatagtggttaatactttcgaagagcttgaaccggagtacgttgaa 720 gaatacaaaacggttaaagataagaagatgtggtgtatcggcccggtttcgttatgcaac 780 aaaaccgggccggatttagccgagcgaggaaacaaagctgcaataaccgaacacaactgc 840 ttaaaatggctcgatgagagaaaactggggtccgtgttatacgtttgtttaggtagcctt 900 gcacgcatttctgccgcacaagcaatcgagctcgggttaggactcgagtccataaaccgt 960 ccctttatatggtgcgtaagaaacgaaaccgatgagctcaaaacatggtttttggatggg 1020 tttgaagaaagggttagagatcgcgggttgatcgttcatggttgggcgccacaggttttg 1080 atactgtcgcacccaaccattggcggtttcttaacccattgcggttggaactcgactatt 1140 gaatcgattaccgcgggtgttccaatgatcacgtggccattttttgcggaccagtttttg 1200 aatgaagcttttatagttgaagttttgaagattggagttaggattggtgttgagagggct 1260 tgtttgtttggggaagaagataaggttggagtgttggtgaagaaggaggatgtgaagaag 1320 gctgttgaatgcttgatggatgaagatgaagatggtgatcagagaagaaagagggtgatt 1380 gagcttgcaaaaatggcgaagattgcaatggcggaaggtggatcttcttatgaaaatgta 1440 tcgtcgttgattcgagatgtgactgaaacagttagagcaccacattag 1488 SEQIDNO:141 Steviarebaudiana MSPKMVAPPTNLHFVLFPLMAQGHLVPMVDIARILAQRGATVTIITTPYHANRVRPVISR 60 AIATNLKIQLLELQLRSTEAGLPEGCESFDQLPSFEYWKNISTAIDLLQQPAEDLLRELS 120 PPPDCIISDFLFPWTTDVARRLNIPRLVFNGPGCFYLLCIHVAITSNILGENEPVSSNTE 180 RVVLPGLPDRIEVTKLQIVGSSRPANVDEMGSWLRAVEAEKASFGIVVNTFEELEPEYVE 240 EYKTVKDKKMWCIGPVSLCNKTGPDLAERGNKAAITEHNCLKWLDERKLGSVLYVCLGSL 300 ARISAAQAIELGLGLESINRPFIWCVRNETDELKTWFLDGFEERVRDRGLIVHGWAPQVL 360 ILSHPTIGGFLTHCGWNSTIESITAGVPMITWPFFADQFLNEAFIVEVLKIGVRIGVERA 420 CLFGEEDKVGVLVKKEDVKKAVECLMDEDEDGDQRRKRVIELAKMAKIAMAEGGSSYENV 480 SSLIRDVTETVRAPH 495 SEQIDNO:142 Arabidopsisthaliana atgggagagaaagcgaaagcaaatgtgttagtcttctcatttccgatacaaggtcacata 60 aaccctctcctccaattctcaaaacgcctactctctaaaaacgtcaacgtcacattcctc 120 accacttcctccacccacaactccatcctccgccgtgccatcaccggcggagccactgct 180 cttcctctctcttttgtccccattgacgatggattcgaggaagatcacccatctacggac 240 acatctcccgactacttcgcaaagttccaagaaaacgtatctcgaagcctctcagagctt 300 atctcctcgatggacccaaaaccaaacgccgtcgtttacgactcgtgcctgccttatgtc 360 ctcgacgtttgccggaaacatcctggcgttgctgcggcgtcgtttttcactcagtcctcc 420 accgtgaacgcgacctatattcatttcttgcgtggagagtttaaggagtttcaaaatgat 480 gtcgttttgcctgcaatgcctccgctgaagggtaatgacttaccggtgtttctgtacgat 540 aacaatctctgccggccgttgtttgagctcattagtagccagttcgtgaatgttgacgac 600 attgacttcttcttggttaactctttcgacgaactcgaagtcgaggtgctacaatggatg 660 aaaaaccaatggccggtcaagaacataggaccgatgattccatcaatgtacttagacaaa 720 cgattagcaggtgacaaagactacggaatcaacctcttcaatgcccaagtcaacgaatgc 780 cttgattggcttgactcaaaaccgcccggttcagtgatctacgtgtcttttggaagcttg 840 gccgtcttaaaagacgatcaaatgatagaagtcgcggctggtctaaaacaaactggccat 900 aacttcttatgggttgttagagaaactgaaacaaagaagcttccaagcaattacatagag 960 gacatttgtgacaagggattgatagtgaattggagtcctcaattacaagttcttgcacat 1020 aaatcaatcggttgtttcatgactcattgcgggtggaattcgactttagaggcattgagc 1080 ttaggagttgctttgataggaatgccggcttatagcgaccagccgactaatgctaagttt 1140 attgaagatgtgtggaaggttggggttagggttaaggcagatcaaaatgggtttgttccg 1200 aaggaagagattgtgagatgtgttggagaagttatggaagatatgtcggagaaagggaag 1260 gagattagaaaaaatgctcggaggttgatggagtttgcaagggaagctttgtctgatgga 1320 ggaaattctgataagaatattgatgagtttgttgctaaaattgtgaggtaa 1371 SEQIDNO:143 Arabidopsisthaliana MGEKAKANVLVFSFPIQGHINPLLQFSKRLLSKNVNVTFLTTSSTHNSILRRAITGGATA 60 LPLSFVPIDDGFEEDHPSTDTSPDYFAKFQENVSRSLSELISSMDPKPNAVVYDSCLPYV 120 LDVCRKHPGVAAASFFTQSSTVNATYIHFLRGEFKEFQNDVVLPAMPPLKGNDLPVFLYD 180 NNLCRPLFELISSQFVNVDDIDFFLVNSFDELEVEVLQWMKNQWPVKNIGPMIPSMYLDK 240 RLAGDKDYGINLFNAQVNECLDWLDSKPPGSVIYVSFGSLAVLKDDQMIEVAAGLKQTGH 300 NFLWVVRETETKKLPSNYIEDICDKGLIVNWSPQLQVLAHKSIGCFMTHCGWNSTLEALS 360 LGVALIGMPAYSDQPTNAKFIEDVWKVGVRVKADQNGFVPKEEIVRCVGEVMEDMSEKGK 420 EIRKNARRLMEFAREALSDGGNSDKNIDEFVAKIVR 456 SEQIDNO:144 Arabidopsisthaliana atggcgccaccgcattttctactggtaacgtttccggcgcaaggtcacgtgaacccatct 60 ctccgttttgctcgtcggctcatcaaaagaaccggcgcacgtgtcactttcgtcacttgt 120 gtctccgtcttccacaactccatgatcgcaaaccacaacaaagtcgaaaatctctctttc 180 cttactttctccgacggtttcgacgatggaggcatttccacctacgaagaccgtcagaaa 240 aggtcggtgaatctcaaggttaacggcgataaggcactatcggatttcatcgaagctact 300 aagaatggtgactctcccgtgacttgcttgatctacacgattcttctcaattgggctcca 360 aaagtagcacgtagatttcaacttccctccgctcttctctggatccaaccggctttggtt 420 ttcaacatctattacactcatttcatgggaaacaagtccgttttcgagttacctaatctg 480 tcttctctggaaatcagagatcttccatctttcctcacaccttccaacacaaacaaaggc 540 gcatacgatgcgtttcaagaaatgatggagtttctcataaaagaaaccaaaccgaaaatt 600 ctcatcaacactttcgattcgctggaaccagaggccttaacggctttcccgaatatcgat 660 atggtggcggttggtcctttacttcccacggagattttctcaggaagcaccaacaaatca 720 gttaaagatcaaagtagtagttatacactttggctagactcgaaaacagagtcctctgtt 780 atttacgtttcctttggaacaatggttgagttgtccaagaaacagatagaggaactagcg 840 agagcactcatagaagggaaacgaccgtttttgtgggttataactgataaatccaacaga 900 gaaacgaaaacagaaggagaagaagagacagagattgagaagatagctggattcagacac 960 gagcttgaagaggttgggatgattgtgtcgtggtgttcgcagatagaggttttaagtcac 1020 cgagccgtaggttgttttgtgactcattgtgggtggagctcgacgctggagagtttggtt 1080 cttggcgttccggttgtggcgtttccgatgtggtcggatcaaccgacgaacgcgaagcta 1140 ctggaagaaagttggaagactggtgtgagggtaagagagaacaaggatggtttggtggag 1200 agaggagagatcaggaggtgtttggaagccgtgatggaggagaagtcggtggagttgagg 1260 gaaaacgcaaagaaatggaagcgtttagcgatggaagcgggtagagaaggaggatcttcg 1320 gataagaacatggaggcttttgtggaggatatttgtggagaatctcttattcaaaacttg 1380 tgtgaagcagaggaggtaaaagtacgctag 1410 SEQIDNO:145 Arabidopsisthaliana MAPPHFLLVTFPAQGHVNPSLRFARRLIKRTGARVTFVTCVSVFHNSMIANHNKVENLSF 60 LTFSDGFDDGGISTYEDRQKRSVNLKVNGDKALSDFIEATKNGDSPVTCLIYTILLNWAP 120 KVARRFQLPSALLWIQPALVFNIYYTHFMGNKSVFELPNLSSLEIRDLPSFLTPSNTNKG 180 AYDAFQEMMEFLIKETKPKILINTFDSLEPEALTAFPNIDMVAVGPLLPTEIFSGSTNKS 240 VKDQSSSYTLWLDSKTESSVIYVSFGTMVELSKKQIEELARALIEGKRPFLWVITDKSNR 300 ETKTEGEEETEIEKIAGFRHELEEVGMIVSWCSQIEVLSHRAVGCFVTHCGWSSTLESLV 360 LGVPVVAFPMWSDQPTNAKLLEESWKTGVRVRENKDGLVERGEIRRCLEAVMEEKSVELR 420 ENAKKWKRLAMEAGREGGSSDKNMEAFVEDICGESLIQNLCEAEEVKVR 469 SEQIDNO:146 Gardeniajasminoides atggttcaacaaagacacgttttgttgattacctatccagctcaaggtcatattaaccca 60 gctttacaattcgcccaaagattattgagaatgggtatccaagttaccttggctacttct 120 gtttatgccttgtccagaatgaagaagtcatctggttctactccaaagggtttgactttt 180 gctactttctctgatggttacgatgatggttttagacctaagggtgttgatcacaccgaa 240 tatatgtcatctttggctaagcaaggttccaacactttgagaaacgttattaacacctct 300 gctgatcaaggttgtccagttacttgtttggtttacactttgttgttgccatgggctgct 360 actgttgctagagaatgtcatattccatctgccttgttgtggattcaaccagttgctgtt 420 atggacatctattactactacttcagaggttacgaagatgacgtcaagaacaattctaat 480 gatccaacctggtccattcaatttccaggtttgccatctatgaaggctaaagatttgcct 540 tcctttatcttgccatcctccgataatatctactcttttgctttgccaaccttcaagaag 600 caattggaaactttggacgaagaagaaagaccaaaggttttggttaataccttcgatgct 660 ttggaaccacaagccttgaaagctattgaatcttacaacttgattgccatcggtccattg 720 actccatctgcttttttggatggtaaagatccatccgaaacatccttttctggtgacttg 780 tttcaaaagtccaaggactacaaagaatggttgaactctagaccagcaggttctgttgtt 840 tacgtttcttttggttccttgttgaccttgccaaagcaacaaatggaagaaattgctaga 900 ggtttgttgaagtctggtagaccatttttgtgggttatcagagctaaagaaaacggtgaa 960 gaagaaaaagaagaagatagattgatctgcatggaagaattggaagaacaaggtatgata 1020 gttccatggtgctcccaaattgaagttttgactcatccatctttgggttgcttcgttact 1080 cattgtggttggaatagtactttggaaaccttggtttgtggtgttccagttgttgcattt 1140 ccacattggaccgatcaaggtactaatgccaaattgattgaagatgtttgggaaaccggt 1200 gttagagttgttccaaatgaagatggtactgtcgaatctgacgaaatcaagagatgtatc 1260 gaaaccgttatggatgatggtgaaaaaggtgtcgaattgaagagaaatgccaagaagtgg 1320 aaagaattggctagagaagctatgcaagaagatggttcttctgacaagaatttgaaggct 1380 ttcgttgaagatgctggtaaaggttatcaagccgaatctaactga 1425 SEQIDNO:147 Gardeniajasminoides MVQQRHVLLITYPAQGHINPALQFAQRLLRMGIQVTLATSVYALSRMKKSSGSTPKGLTF 60 ATFSDGYDDGFRPKGVDHTEYMSSLAKQGSNTLRNVINTSADQGCPVTCLVYTLLLPWAA 120 TVARECHIPSALLWIQPVAVMDIYYYYFRGYEDDVKNNSNDPTWSIQFPGLPSMKAKDLP 180 SFILPSSDNIYSFALPTFKKQLETLDEEERPKVLVNTFDALEPQALKAIESYNLIAIGPL 240 TPSAFLDGKDPSETSFSGDLFQKSKDYKEWLNSRPAGSVVYVSFGSLLTLPKQQMEEIAR 300 GLLKSGRPFLWVIRAKENGEEEKEEDRLICMEELEEQGMIVPWCSQIEVLTHPSLGCFVT 360 HCGWNSTLETLVCGVPVVAFPHWTDQGTNAKLIEDVWETGVRVVPNEDGTVESDEIKRCI 420 ETVMDDGEKGVELKRNAKKWKELAREAMQEDGSSDKNLKAFVEDAGKGYQAESN 474 SEQIDNO:152 Arabidopsisthaliana atggaggaaaagcctgcaaggagaagcgtagtgttggttccatttccagcacaaggacat 60 atatctccaatgatgcaacttgccaaaacccttcacttaaagggtttctcgatcacagtt 120 gttcagactaagttcaattactttagcccttcagatgacttcactcatgattttcagttc 180 gtcaccattccagaaagcttaccagagtctgatttcaagaatctcggaccaatacagttt 240 ctgtttaagctcaacaaagagtgtaaggtgagcttcaaggactgtttgggtcagttggtg 300 ctgcaacaaagtaatgagatctcatgtgtcatctacgatgagttcatgtactttgctgaa 360 gctgcagccaaagagtgtaagcttccaaacatcattttcagcacaacaagtgccacggct 420 ttcgcttgccgctctgtatttgacaaactatatgcaaacaatgtccaagctcccttgaaa 480 gaaactaaaggacaacaagaagagctagttccggagttttatcccttgagatataaagac 540 tttccagtttcacggtttgcatcattagagagcataatggaggtgtataggaatacagtt 600 gacaaacggacagcttcctcggtgataatcaacactgcgagctgtctagagagctcatct 660 ctgtcttttctgcaacaacaacagctacaaattccagtgtatcctataggccctcttcac 720 atggtggcctcagctcctacaagtctgcttgaagagaacaagagctgcatcgaatggttg 780 aacaaacaaaaggtaaactcggtgatatacataagcatgggaagcatagctttaatggaa 840 atcaacgagataatggaagtcgcgtcaggattggctgctagcaaccaacacttcttatgg 900 gtgatccgaccagggtcaatacctggttccgagtggatagagtccatgcctgaagagttt 960 agtaagatggttttggaccgaggttacattgtgaaatgggctccacagaaggaagtactt 1020 tctcatcctgcagtaggagggttttggagccattgtggatggaactcgacactagaaagc 1080 atcggccaaggagttccaatgatctgcaggccattttcgggtgatcaaaaggtgaacgct 1140 agatacttggagtgtgtatggaaaattgggattcaagtggagggtgagctagacagagga 1200 gtggtcgagagagctgtgaagaggttaatggttgacgaagaaggagaggagatgaggaag 1260 agagctttcagtttaaaagagcaacttagagcctctgttaaaagtggaggctcttcacac 1320 aactcgctagaagagtttgtacacttcataaggactgcctag 1362 SEQIDNO:153 Arabidopsisthaliana MEEKPARRSVVLVPFPAQGHISPMMQLAKTLHLKGFSITVVQTKFNYFSPSDDFTHDFQF 60 VTIPESLPESDFKNLGPIQFLFKLNKECKVSFKDCLGQLVLQQSNEISCVIYDEFMYFAE 120 AAAKECKLPNIIFSTTSATAFACRSVFDKLYANNVQAPLKETKGQQEELVPEFYPLRYKD 180 FPVSRFASLESIMEVYRNTVDKRTASSVIINTASCLESSSLSFLQQQQLQIPVYPIGPLH 240 MVASAPTSLLEENKSCIEWLNKQKVNSVIYISMGSIALMEINEIMEVASGLAASNQHFLW 300 VIRPGSIPGSEWIESMPEEFSKMVLDRGYIVKWAPQKEVLSHPAVGGFWSHCGWNSTLES 360 IGQGVPMICRPFSGDQKVNARYLECVWKIGIQVEGELDRGVVERAVKRLMVDEEGEEMRK 420 RAFSLKEQLRASVKSGGSSHNSLEEFVHFIRTA 453 SEQIDNO:168 Catharanthusroseus atggcaactgaacaacaacaagcatctatctcctgcaaaatcttaatgtttccttggtta 60 gccttcggtcatatctcttctttcttacaattggctaagaaattgtctgatagaggtttc 120 tacttctacatttgtagtactccaattaatttggactctattaaaaataagataaaccaa 180 aactattcttcatccatacaattggttgatttgcatttgccaaacagtcctcaattgcca 240 ccttctttacatactacaaatggtttgccacctcacttaatgtctacattgaaaaacgct 300 ttgatcgatgcaaatccagacttatgcaagattatagcctcaattaaaccagatttgatc 360 atctatgacttacatcaaccttggaccgaagcattggcttctagacacaacattcctgct 420 gttagtttttctactatgaatgccgtatcctttgcttacgttatgcacatgttcatgaat 480 ccaggtatagaatttcctttcaaagcaatccacttatcagattttgaacaagccagattc 540 ttggaacaattagaatcagctaagaacgatgcctccgctaaagacccagaattgcaaggt 600 agtaagggtttctttaactctaccttcattgttagaagttctagagaaatcgagggtaaa 660 tacgttgattacttgtcagaaatcttaaagtccaaggtcattccagtatgtcctgttata 720 tctttgaataacaacgatcaaggtcagggtaacaaagatgaagacgaaataatccaatgg 780 ttagacaaaaagtctcatagatcatccgtatttgtttcattcggttccgaatactttttg 840 aacatgcaagaaatcgaagaaatcgctataggtttggaattatctaacgtcaactttata 900 tgggtattgagattcccaaagggtgaagatacaaaaattgaagaagttttgcctgaaggt 960 ttcttggacagagttaaaaccaagggtagaattgtccacggttgggcaccacaagccaga 1020 atcttgggtcatccttcaattggtggtttcgtatcccactgcggttggaatagtgttatg 1080 gaatctatccaaatcggtgtcccaattatagcaatgcctatgaacttggatcaacctttt 1140 aatgccagattagttgtcgaaatcggtgtcggtattgaagtaggtagagatgaaaacggt 1200 aaattaaagagagaaagaatcggtgaagttatcaaggaagtcgctataggtaaaaagggt 1260 gaaaaattgagaaagacagcaaaagatttgggtcaaaaattgagagatagagaaaaacaa 1320 gactttgacgaattagcagcaactttgaaacaattatgcgtatga 1365 SEQIDNO:169 Catharanthusroseus MATEQQQASISCKILMFPWLAFGHISSFLQLAKKLSDRGFYFYICSTPINLDSIKNKINQ 60 NYSSSIQLVDLHLPNSPQLPPSLHTTNGLPPHLMSTLKNALIDANPDLCKIIASIKPDLI 120 IYDLHQPWTEALASRHNIPAVSFSTMNAVSFAYVMHMFMNPGIEFPFKAIHLSDFEQARF 180 LEQLESAKNDASAKDPELQGSKGFFNSTFIVRSSREIEGKYVDYLSEILKSKVIPVCPVI 240 SLNNNDQGQGNKDEDEIIQWLDKKSHRSSVFVSFGSEYFLNMQEIEEIAIGLELSNVNFI 300 WVLRFPKGEDTKIEEVLPEGFLDRVKTKGRIVHGWAPQARILGHPSIGGFVSHCGWNSVM 360 ESIQIGVPIIAMPMNLDQPFNARLVVEIGVGIEVGRDENGKLKRERIGEVIKEVAIGKKG 420 EKLRKTAKDLGQKLRDREKQDFDELAATLKQLCV 454 SEQIDNO:172 Arabidopsisthaliana atgaccaaattctccgagccaatcagagactcccacgtggcagttctcgcgtttttcccc 60 gttggcgctcatgccggtcctctcttagccgtcactcgccgtctcgccgccgcttctccc 120 tccaccatcttttctttcttcaacaccgcaagatcaaacgcgtcgttgttctcctctgat 180 catcccgagaacatcaaggtccacgacgtctctgacggtgttccggagggaaccatgctc 240 gggaatccactggagatggtcgagctgtttctcgaagcggctccacgtattttccggagc 300 gaaatcgcggcggcagagatagaagttggaaagaaagtgacatgcatgctaacagatgcc 360 ttcttctggttcgcagcggacatagcggctgagctgaacgcgacttgggttgccttctgg 420 gccggcggagcaaactcactctgtgctcatctctacactgatctcatcagagaaaccatc 480 ggtctcaaagatgtgagtatggaagagacattagggtttataccaggaatggagaattac 540 agagttaaagatataccagaggaagttgtatttgaagatttggactctgttttcccaaag 600 gctttataccaaatgagtcttgctttacctcgtgcctctgctgttttcatcagttccttt 660 gaagagttagaacctacattgaactataacctaagatccaaacttaaacgtttcttgaac 720 atcgcccctctcacgttattatcttctacatcggagaaagagatgcgtgatcctcatggc 780 tgctttgcttggatggggaagagatcagctgcttctgtagcgtacattagcttcggcacc 840 gtcatggaacctcctcctgaagagcttgtggcgatagcacaagggttggaatcaagcaaa 900 gtgccgtttgtttggtcgctgaaggagaagaacatggttcatctaccaaaagggtttttg 960 gatcggacaagagagcaagggatagtggttccttgggctccacaagtggaactgctgaaa 1020 cacgaggcaatgggtgtgaatgtgacacattgtggatggaactcagtgttggagagtgtg 1080 tcggcaggtgtaccgatgatcggcagaccgattttggcggataataggctcaacggaaga 1140 gcagtggaggttgtgtggaaggttggagtgatgatggataatggagtcttcacgaaagaa 1200 ggatttgagaagtgtttgaatgatgtttttgttcatgatgatggtaagacgatgaaggct 1260 aatgccaagaagcttaaagaaaaactccaagaagatttctccatgaaaggaagctcttta 1320 gagaatttcaaaatattgttggacgaaattgtgaaagtttag 1362 SEQIDNO:173 Arabidopsisthaliana MTKFSEPIRDSHVAVLAFFPVGAHAGPLLAVTRRLAAASPSTIFSFFNTARSNASLFSSD 60 HPENIKVHDVSDGVPEGTMLGNPLEMVELFLEAAPRIFRSEIAAAEIEVGKKVTCMLTDA 120 FFWFAADIAAELNATWVAFWAGGANSLCAHLYTDLIRETIGLKDVSMEETLGFIPGMENY 180 RVKDIPEEVVFEDLDSVFPKALYQMSLALPRASAVFISSFEELEPTLNYNLRSKLKRFLN 240 IAPLTLLSSTSEKEMRDPHGCFAWMGKRSAASVAYISFGTVMEPPPEELVAIAQGLESSK 300 VPFVWSLKEKNMVHLPKGFLDRTREQGIVVPWAPQVELLKHEAMGVNVTHCGWNSVLESV 360 SAGVPMIGRPILADNRLNGRAVEVVWKVGVMMDNGVFTKEGFEKCLNDVFVHDDGKTMKA 420 NAKKLKEKLQEDFSMKGSSLENFKILLDEIVKV 453 SEQIDNO:176 Streptomycesantibioticus atgacttctgaacatagatccgcttccgttactccaagacatatttcattcttcaacatc 60 ccaggtcatggtcatgttaatccatctttgggtatcgttcaagaattggttgctagaggt 120 cacagagtttcttacgctattaccgatgaatttgctgctcaagttaaggctgctggtgct 180 actccagttgtttatgattccatcttgccaaaagaatccaacccagaagaatcttggcca 240 gaagatcaagaatctgctatgggtttgttcttggatgaagctgttagagtcttgccacaa 300 ttagaagatgcttacgctgatgatagaccagatttgatcgtttacgatattgcttcttgg 360 ccagctccagttttgggtagaaaatgggatattccattcgtccaattatccccaactttc 420 gttgcttacgaaggttttgaagaagatgttccagcagttcaagatccaactgctgataga 480 ggtgaagaagctgctgctccagcaggtactggtgatgctgaagaaggtgctgaagctgaa 540 gatggtttggttagattcttcactagattgtccgctttcttggaagaacatggtgttgat 600 actccagctaccgaatttttgattgctccaaacagatgcatcgttgctttgccaagaact 660 tttcaaatcaagggtgataccgttggtgataactacacttttgttggtccaacttacggt 720 gatagatctcatcaaggtacttgggaaggtccaggtgatggtagaccagttttgttgatt 780 gctttgggttctgctttcactgatcacttggatttctacagaacctgtttgtctgctgtt 840 gatggtttggattggcatgttgttttgtctgttggtagatttgttgatccagcagatttg 900 ggtgaagttccaccaaatgttgaagttcatcaatgggttccacaattagatattttgacc 960 aaggcttccgccttcattactcatgctggtatgggttctactatggaagccttgtctaat 1020 gctgttccaatggttgctgttccacaaattgctgaacaaactatgaacgccgaaagaata 1080 gtcgaattgggtttgggtagacatatcccaagagatcaagttactgccgaaaaattgaga 1140 gaagctgttttggctgttgcttctgatccaggtgttgctgaaagattggctgctgttaga 1200 caagaaattagagaagccggtggtgctagagctgctgctgatattttggaaggtattttg 1260 gctgaagccggttaa 1275 SEQIDNO:177 Streptomycesantibioticus MTSEHRSASVTPRHISFFNIPGHGHVNPSLGIVQELVARGHRVSYAITDEFAAQVKAAGA 60 TPVVYDSILPKESNPEESWPEDQESAMGLFLDEAVRVLPQLEDAYADDRPDLIVYDIASW 120 PAPVLGRKWDIPFVQLSPTFVAYEGFEEDVPAVQDPTADRGEEAAAPAGTGDAEEGAEAE 180 DGLVRFFTRLSAFLEEHGVDTPATEFLIAPNRCIVALPRTFQIKGDTVGDNYTFVGPTYG 240 DRSHQGTWEGPGDGRPVLLIALGSAFTDHLDFYRTCLSAVDGLDWHVVLSVGRFVDPADL 300 GEVPPNVEVHQWVPQLDILTKASAFITHAGMGSTMEALSNAVPMVAVPQIAEQTMNAERI 360 VELGLGRHIPRDQVTAEKLREAVLAVASDPGVAERLAAVRQEIREAGGARAAADILEGIL 420 AEAG 424 SEQIDNO:180 Oryzasativa atgaagcaaaccgtcgtcctgtaccccggcggcggcgtcggccacgtcgtccccatgctg 60 gagctcgccaaggtcttcgtcaagcacgggcacgacgtcaccatggtgctgctggagccg 120 cccttcaagtcgtccgactccggcgccctcgccgtcgagcgcctcgtcgcctccaaccct 180 tccgtctccttccacgtcctcccgccactccccgcccccgacttcgccagcttcggcaag 240 cacccgttcctcctcgtcatccagctcctgcgccagtacaacgagcggctcgagagcttc 300 ctcctctccatccctcgacagcgcctgcactccctcgtcatcgacatgttctgcgtcgac 360 gccatcgacgtgtgcgcaaagctcggcgtgccggtgtacacgttcttcgcctcgggcgtc 420 tcggtgctgtccgtcttgacccagctcccaccgtttcttgccggtagggagacgggcctg 480 aaggagcttggcgacacgccgcttgatttcctcggtgtttcgccgatgccggcgtctcat 540 ctcgtcaaggaattgctcgagcatccggaggacgagttgtgcaaggccatggtgaaccgc 600 tgggagcgcaacacggaaaccatgggcgtcctggtgaactcgttcgaatcgttggagagc 660 cgggcggctcaggcgctcagggacgacccgctctgcgtcccaggcaaggtgctgcctccg 720 atctactgcgtcgggcctttggtcggcggcggcgcggaggaggcggccgagaggcacgag 780 tgcctcgtctggctcgacgctcagccggagcacagcgtcgtgttcctctgcttcgggagc 840 aagggcgtgttctccgcggagcagctcaaggagatcgccgtcggcttggagaactccagg 900 caacggttcatgtgggtcgtgcgcacgccgccgacaaccaccgaaggcttgaagaagtac 960 ttcgagcaacgcgcggcgccggacctcgacgcgctcttcccggatgggttcgtggagcgt 1020 accaaggaccgtggcttcatcgtcacgacgtgggcgccgcaggtggacgtgctccgccac 1080 cgggcgaccggcgcgttcgtgacgcactgcgggtggaactcggcgctggagggcatcacg 1140 gcgggggtgccgatgctgtgctggccgcagtacgcggagcagaagatgaacaaggtgttc 1200 atgacggcggagatgggcgtcggggtggagctggacgggtacaactcggactttgtcaaa 1260 gcggaggagttggaggccaaggtgaggctggtgatggagtcggaggaagggaagcagctc 1320 agggctcgttcggctgcgcggaagaaggaggcagaggcggcgctggaggaagggggctcg 1380 tcgcacgctgcgttcgtccagttcctgtccgatgtggagaatcttgtccagaactaa 1437 SEQIDNO:181 Oryzasativa MKQTVVLYPGGGVGHVVPMLELAKVFVKHGHDVTMVLLEPPFKSSDSGALAVERLVASNP 60 SVSFHVLPPLPAPDFASFGKHPFLLVIQLLRQYNERLESFLLSIPRQRLHSLVIDMFCVD 120 AIDVCAKLGVPVYTFFASGVSVLSVLTQLPPFLAGRETGLKELGDTPLDFLGVSPMPASH 180 LVKELLEHPEDELCKAMVNRWERNTETMGVLVNSFESLESRAAQALRDDPLCVPGKVLPP 240 IYCVGPLVGGGAEEAAERHECLVWLDAQPEHSVVFLCFGSKGVFSAEQLKEIAVGLENSR 300 QRFMWVVRTPPTTTEGLKKYFEQRAAPDLDALFPDGFVERTKDRGFIVTTWAPQVDVLRH 360 RATGAFVTHCGWNSALEGITAGVPMLCWPQYAEQKMNKVFMTAEMGVGVELDGYNSDFVK 420 AEELEAKVRLVMESEEGKQLRARSAARKKEAEAALEEGGSSHAAFVQFLSDVENLVQN 478 SEQIDNO:182 Nicotianatabacum atgactactcaaaaagctcattgcttgatcttaccatatccagctcagggtcatatcaac 60 cctatgctccaattctccaaacgtttgcaatccaaaggtgtcaaaatcactatagcagcc 120 accaaatcattcttgaaaaccatgcaagaattgtcaacttctgtgtcagtcgaggctatc 180 tccgatggctatgatgatggcggacgcgagcaagctggaacctttgtggcctatattaca 240 agattcaaagaagttggctcggatactttgtctcagcttattggaaagttaacaaattgt 300 ggttgtcctgtgagttgcatagtttacgatccatttcttccttgggctgttgaagtggga 360 aataattttggagtagctactgctgcttttttcactcaatcttgtgcagtggataacatt 420 tattaccatgtacataaaggggttctaaaacttcctccaactgacgttgataaagaaatc 480 tcaattcctggattattaacaattgaggcatcagatgtacctagttttgtttctaatcct 540 gaatcttcaagaatacttgaaatgttggtgaatcagttctcgaatcttgagaacacagat 600 tgggtcctaatcaacagtttctatgaattggagaaagaggtaattgattggatggccaag 660 atctatccaatcaagacaattggaccaactataccatcaatgtacctagacaagaggcta 720 ccagatgacaaagaatatggccttagtgtcttcaagccaatgacaaatgcatgcctaaac 780 tggttaaaccatcaaccagttagctcagtagtatatgtatcatttggaagtttagccaaa 840 ttagaagcagagcaaatggaagaattagcatggggtttgagtaatagcaacaagaacttc 900 ttgtgggtagttagatccactgaagaatccaaacttcccaacaactttttagaggaatta 960 gcaagtgaaaaaggattagtcgtgtcatggtgtccacaattacaagtcttggaacataaa 1020 tcaatagggtgttttctcacgcactgtggctggaattcaactttggaagcaattagtttg 1080 ggagtaccaatgattgcaatgccacattggtcagaccagccaacaaatgcgaagcttgtg 1140 gaagatgtttgggagatgggaattagaccaaaacaagatgaaaaaggattagttagaaga 1200 gaagttattgaagaatgtattaagatagtgatggaggaaaagaaaggaaaaaagattagg 1260 gaaaatgcaaagaaatggaaggaattggctaggaaagctgtggatgaaggaggaagttca 1320 gatagaaatattgaagaatttgtttccaagttggtgactattgcctcagtggaaagctaa 1380 SEQIDNO:183 Nicotianatabacum MTTQKAHCLILPYPAQGHINPMLQFSKRLQSKGVKITIAATKSFLKTMQELSTSVSVEAI 60 SDGYDDGGREQAGTFVAYITRFKEVGSDTLSQLIGKLTNCGCPVSCIVYDPFLPWAVEVG 120 NNFGVATAAFFTQSCAVDNIYYHVHKGVLKLPPTDVDKEISIPGLLTIEASDVPSFVSNP 180 ESSRILEMLVNQFSNLENTDWVLINSFYELEKEVIDWMAKIYPIKTIGPTIPSMYLDKRL 240 PDDKEYGLSVFKPMTNACLNWLNHQPVSSVVYVSFGSLAKLEAEQMEELAWGLSNSNKNF 300 LWVVRSTEESKLPNNFLEELASEKGLVVSWCPQLQVLEHKSIGCFLTHCGWNSTLEAISL 360 GVPMIAMPHWSDQPTNAKLVEDVWEMGIRPKQDEKGLVRREVIEECIKIVMEEKKGKKIR 420 ENAKKWKELARKAVDEGGSSDRNIEEFVSKLVTIASVES 459 SEQIDNO:184 Siraitiagrosvenorii atggagaaaggcgatacgcatattctagtgtttcctttcccttcacaaggccacataaac 60 cctcttcttcaactatcgaagcgcctaatcgccaagggaatcaaggtttcgctggtcaca 120 accttacatgttagcaatcacttgcagttgcagggtgcttattccaactccgtgaagatc 180 gaagtcatttccgatggctctgaggatcgtctggaaaccgatactatgcgccaaactctg 240 gatcgatttcggcagaagatgacgaagaacttggaagatttcttgcagaaagccatggtt 300 tcttcaaatccgcctaaattcattctgtatgattcgacaatgccgtgggttttggaggtc 360 gccaaggagttcggactcgatagggccccgttctacactcagtcttgtgcgcttaacagt 420 atcaattatcatgttcttcatggtcaattgaagcttcctcctgaaacccccacgatttcg 480 ttgccttctatgcctctgcttcgccccagcgatctcccggcttatgattttgatcctgcc 540 tccactgacaccatcatcgatcttcttaccagtcagtattctaatatccaggatgcaaat 600 ctgcttttctgcaacacttttgacaagttggaaggcgagattatccaatggatggagacc 660 ctgggtcgccctgtgaaaaccgtaggaccaactgttccatcagcctacttagacaaaagg 720 gtagagaacgacaagcactatgggctgagtctgttcaagcccaacgaggacgtctgcctc 780 aaatggcttgatagcaagccctctggttctgttctgtatgtgtcttatggcagtttggtt 840 gaaatgggggaagagcagctgaaggagttggctctgggaatcaaggaaactggcaagttc 900 ttcttgtgggtggtgagagacactgaagcagagaagcttcctcccaactttgtggagagt 960 gtggcagagaaggggcttgtggtcagctggtgctcccagctggaggtattggctcacccc 1020 tccgtcggctgcttcttcacgcactgtggctggaactcgacgcttgaggcgctgtgcttg 1080 ggcgtcccggtggtcgctttcccacagtgggctgatcaggtaaccaatgcaaagttttta 1140 gaagatgtttggaaggttgggaagagggtgaagcggaatgagcagaggctggcaagtaaa 1200 gaagaagtaaggagttgcatttgggaagtgatggagggagagagagccagcgagttcaag 1260 agcaactccatggagtggaagaagtgggcaaaagaagctgtggatgaaggtgggagctct 1320 gataagaacattgaggagtttgtggctatgctcaagcaaacttga 1365 SEQIDNO:185 Siraitiagrosvenorii MEKGDTHILVFPFPSQGHINPLLQLSKRLIAKGIKVSLVTTLHVSNHLQLQGAYSNSVKI 60 EVISDGSEDRLETDTMRQTLDRFRQKMTKNLEDFLQKAMVSSNPPKFILYDSTMPWVLEV 120 AKEFGLDRAPFYTQSCALNSINYHVLHGQLKLPPETPTISLPSMPLLRPSDLPAYDFDPA 180 STDTIIDLLTSQYSNIQDANLLFCNTFDKLEGEIIQWMETLGRPVKTVGPTVPSAYLDKR 240 VENDKHYGLSLFKPNEDVCLKWLDSKPSGSVLYVSYGSLVEMGEEQLKELALGIKETGKF 300 FLWVVRDTEAEKLPPNFVESVAEKGLVVSWCSQLEVLAHPSVGCFFTHCGWNSTLEALCL 360 GVPVVAFPQWADQVTNAKFLEDVWKVGKRVKRNEQRLASKEEVRSCIWEVMEGERASEFK 420 SNSMEWKKWAKEAVDEGGSSDKNIEEFVAMLKQT 454 SEQIDNO:198 Crocussativus atggggtcagaagataggtccttgtccatcttattctttccttttatggcacaaggtcac 60 atgttacctatgctagatatggctaagttatttgctctgtatggtgtcaaatcaacagta 120 gtgaccactccagctaatgtaccaatagtcaactcagtaattgatcagcctgatgtttct 180 actttgcacccaatccaattacgactgataccatttccatctgacacgggcttgcctgaa 240 ggttgtgaaaacgtatcatcaattcctccaagagacatgccaactgttcatgtcactttc 300 ttcagcgctacagcaaaacttagagaaccttttggtaaggtgctagaggatctaagacca 360 gattgtattgttactgacatgtttttcccttggacctacgatgtggccgcagaattaggt 420 atcccaaggattgttttccatgggacaaatttcttttctctctgcgtaacagattctctt 480 gaaagatataaaccagttgaaaacttgcgaagtgatgccgagtctgtagtgatcccagga 540 ctcccacacagaatcgaggtattgcgttctcaaataccagaatacgaaaaatcaaaagca 600 gattttgttagagaagttagggaatcagaatctaagtcttacggagcggtggttaattct 660 ttctttgaattggaacctgactacgctagacattacagagaggttgtcggcagacgtgct 720 tggcatatcgggccacttgctctggtcaataactctactacagacaaaagctcaagagga 780 tacaagacagcgatcgatagaaacgattgtttgaaatggctcgattctaaaagactaaga 840 tccgttgtatatgtgtgctttggctcaatgtctgacttttccgatgcccaattacgtgaa 900 atggcaagtggtctagaggcatccaatcatcctttcatttgggtggttagaaaatctggc 960 aaggaatggttaccagaaggatttgaggaaagagtccaggagagaggtttgattatcaga 1020 ggctgggctccacaaatcttaatactcaaccatagagcagtgggaggcttcatgacccat 1080 tgtgggtggaatagtagtttggaagcagtttctgccggactgcctcttgttacatggcct 1140 ctatttgcagaacaattttacaatgaaagattcatggttgatgttttgagaattggtgta 1200 tcagtgggtgcgaagagacacggtatgaaagccgaagagagagaagtcgtagaagccaaa 1260 atggttaaggaagctgttgatggcttgatggacgacggtgaagaggctgagggtagaagg 1320 cgtagagctagagaactgggcgaaaaagctagaaaggccgtcgaaaaaggtggttcatcc 1380 tacgaggacatgagaaatcttttgcaagagcttaagggtgatagcaagttaactgtcgga 1440 tgctaa 1446 SEQIDNO:199 Crocussativus MGSEDRSLSILFFPFMAQGHMLPMLDMAKLFALYGVKSTVVTTPANVPIVNSVIDQPDVS 60 TLHPIQLRLIPFPSDTGLPEGCENVSSIPPRDMPTVHVTFFSATAKLREPFGKVLEDLRP 120 DCIVTDMFFPWTYDVAAELGIPRIVFHGTNFFSLCVTDSLERYKPVENLRSDAESVVIPG 180 LPHRIEVLRSQIPEYEKSKADFVREVRESESKSYGAVVNSFFELEPDYARHYREVVGRRA 240 WHIGPLALVNNSTTDKSSRGYKTAIDRNDCLKWLDSKRLRSVVYVCFGSMSDFSDAQLRE 300 MASGLEASNHPFIWVVRKSGKEWLPEGFEERVQERGLIIRGWAPQILILNHRAVGGFMTH 360 CGWNSSLEAVSAGLPLVTWPLFAEQFYNERFMVDVLRIGVSVGAKRHGMKAEEREVVEAK 420 MVKEAVDGLMDDGEEAEGRRRRARELGEKARKAVEKGGSSYEDMRNLLQELKGDSKLTVG 480 C 481 SEQIDNO:200 Crocussativus atggaggctggaggtgacaaacttcacattgttgtctttccatggttagcttttggccac 60 atgttgccatttctagagctgtctaagtctttggctaaaagaggtcacttaatcagtttt 120 gtttctacacctaaaaacattcaaagatttcctaatcttccaccacaaatctcaccactt 180 atcaactttatcccattaagtctacctaaagtggagggcatgccaggtgacgtagaagct 240 accacagacctaccacctgccaacctacaatatctgaaaaaggcacttgacgggttagaa 300 caacctttcagatcattcctaagagaggcctccccaaaacctgattggataatccaagat 360 cttttacaacattggatacctccaattgccgcagaacttcatgttccttccatgtacttt 420 ggcacagtgccagctgccgccttgacctttttcggtcatccatcacaacttagttcaaga 480 gggaagggattggaaggctggctggcttcaccaccatgggttccattcccatctaaggtg 540 gcatacagattgcacgaactaatcgttatggctaaagatgccgctggtccattgcattcc 600 ggtatgactgatgctagaaggatggaagctgcaatagttggatgctgtgcagtcgctatt 660 agaacatgtagagaattggaatcagaatggttacctattctggaggagatctacggaaag 720 cctgtgataccagttggattacttttacctactgctgatgaatctactgatggaaactct 780 atcatagactggttaggcacaagatcccaggaatcagtagtgtacattgctctgggttca 840 gaagtttctattggtgtggaattgatacatgaattggccttgggtcttgaattagcaggt 900 ttgccattcctatgggcactacgtagaccttatggactgtctagtgatactgagattttg 960 cctggtggattcgaggagagaactagaggctatggaaaggtagtcatgggctgggttcct 1020 caaatgagagtcttggcagatcgttctgtaggcggctttgtcacacactgtggttggtca 1080 tctgtagttgaatcattacattttgggcatccactagttttactgccaatcttcggtgac 1140 caaggattgaatgcaagattgctggaggaaaagggaattggggtcgaagtagaaaggaag 1200 ggtgatgggtcttttacccgtaatgaagttgcaaaagcaatcaatttgatcatggtcgaa 1260 ggtgacggttctggttcctcctacaggaaaaaggcaaaggaaatgaaaaagattttcgct 1320 gataaggaatgccaggagaaatacgtggatgaatttgtgcagttcctgttatcaaatggt 1380 actgctaaaggctaa 1395 SEQIDNO:201 Crocussativus MEAGGDKLHIVVFPWLAFGHMLPFLELSKSLAKRGHLISFVSTPKNIQRFPNLPPQISPL 60 INFIPLSLPKVEGMPGDVEATTDLPPANLQYLKKALDGLEQPFRSFLREASPKPDWIIQD 120 LLQHWIPPIAAELHVPSMYFGTVPAAALTFFGHPSQLSSRGKGLEGWLASPPWVPFPSKV 180 AYRLHELIVMAKDAAGPLHSGMTDARRMEAAIVGCCAVAIRTCRELESEWLPILEEIYGK 240 PVIPVGLLLPTADESTDGNSIIDWLGTRSQESVVYIALGSEVSIGVELIHELALGLELAG 300 LPFLWALRRPYGLSSDTEILPGGFEERTRGYGKVVMGWVPQMRVLADRSVGGFVTHCGWS 360 SVVESLHFGHPLVLLPIFGDQGLNARLLEEKGIGVEVERKGDGSFTRNEVAKAINLIMVE 420 GDGSGSSYRKKAKEMKKIFADKECQEKYVDEFVQFLLSNGTAKG 464 SEQIDNO:202 Arabidopsisthaliana atggagaagatgagaggacatgtattagcagtgccatttccaagccaaggacacatcacc 60 ccgattcgccaattctgcaaacgacttcactccaaaggtttcaaaaccactcacactctc 120 accacttttatcttcaacacaatccacctcgacccatctagtcctatctccatagccaca 180 atctccgatggctatgaccagggagggttctcatcagccggttctgtcccggagtaccta 240 caaaacttcaaaaccttcggctccaaaaccgtcgctgatatcatccgcaaacaccagagt 300 actgataaccctattacttgtatcgtctatgattctttcatgccttgggcgcttgacctt 360 gcaatggattttggtctagctgcggctcctttcttcacgcagtcttgcgccgttaactat 420 atcaattatctttcttacataaacaatggtagcttgacacttcccatcaaggatttgcct 480 cttcttgagctccaagatttgcctactttcgtcactcctactggttcacaccttgcttac 540 tttgagatggtgcttcaacagttcaccaacttcgacaaagctgatttcgtactcgttaat 600 tccttccatgacctcgaccttcatgaagaggagttgttgtcgaaagtatgtcctgtgttg 660 acaattggtccaactgttccatcaatgtacttagaccaacagatcaaatcagacaacgac 720 tatgatctgaacctctttgacttaaaagaagctgccttatgcactgactggctagacaag 780 aggccagaaggatcggtagtatatatagcttttgggagcatggctaaactgagtagtgag 840 cagatggaagagattgcttcggcgataagcaacttcagctacctctgggttgtcagagct 900 tcagaggagtcaaagctcccaccagggtttcttgaaacagtggataaagacaagagcttg 960 gtcttgaagtggagtcctcagcttcaagttctgtcaaacaaagccatcggttgtttcatg 1020 actcactgtggctggaactcaaccatggagggtttgagtttaggggttcccatggtggct 1080 atgcctcaatggactgatcaaccaatgaatgcaaagtatatacaagatgtatggaaggtt 1140 ggggttcgtgtgaaagcagagaaagaaagtggcatttgcaaaagagaggagattgagttt 1200 agcatcaaggaagtgatggaaggagagaagagcaaagagatgaaagagaatgcgggaaaa 1260 tggagagacttggctgtgaagtcactcagtgaaggaggttctacagatatcaacattaac 1320 gaatttgtatcaaaaattcaaatcaaataa 1350 SEQIDNO:203 Arabidopsisthaliana MEKMRGHVLAVPFPSQGHITPIRQFCKRLHSKGFKTTHTLTTFIFNTIHLDPSSPISIAT 60 ISDGYDQGGFSSAGSVPEYLQNFKTFGSKTVADIIRKHQSTDNPITCIVYDSFMPWALDL 120 AMDFGLAAAPFFTQSCAVNYINYLSYINNGSLTLPIKDLPLLELQDLPTFVTPTGSHLAY 180 FEMVLQQFTNFDKADFVLVNSFHDLDLHEEELLSKVCPVLTIGPTVPSMYLDQQIKSDND 240 YDLNLFDLKEAALCTDWLDKRPEGSVVYIAFGSMAKLSSEQMEEIASAISNFSYLWVVRA 300 SEESKLPPGFLETVDKDKSLVLKWSPQLQVLSNKAIGCFMTHCGWNSTMEGLSLGVPMVA 360 MPQWTDQPMNAKYIQDVWKVGVRVKAEKESGICKREEIEFSIKEVMEGEKSKEMKENAGK 420 WRDLAVKSLSEGGSTDININEFVSKIQIK 449 SEQIDNO:204 Arabidopsisthaliana atggccaacaacaattccaactctcccaccggtccacactttctattcgtaacatttcca 60 gcccaaggtcacatcaacccatctctcgagctagccaaacgcctcgccggaacaatctct 120 ggtgctcgagtcaccttcgccgcctcaatctctgcctacaaccgccgcatgttctctaca 180 gaaaacgtccccgaaaccctaatcttcgctacctactccgatggccacgacgacggtttc 240 aaatcctctgcttactccgacaaatctcgtcaagacgccactggaaacttcatgtctgag 300 atgagacgacgtggcaaagagacactaaccgaactaatcgaagataaccggaaacaaaac 360 aggccttttacttgcgtggtttacacgattctcctcacttgggtcgctgagctagcgcgt 420 gagtttcatcttccttctgctcttctttgggtccaaccagtaacagtcttctccattttt 480 taccattacttcaatggctacgaagatgcaatctcagagatggctaataccccctctagt 540 tctattaaattaccttctctgccactgcttactgtccgtgatattccttctttcattgtc 600 tcttccaatgtctacgcgtttcttctacccgcgtttcgagaacagattgattcactgaag 660 gaagaaataaaccctaagatcctcatcaacactttccaagagcttgagccagaagccatg 720 agctcggttccagataatttcaagattgtccctgtcggtccgttactaacgttgagaacg 780 gatttttcgagtcgcggtgaatacatagagtggttggatactaaagcggattcgtctgtg 840 ctttatgtttcgttcgggacgcttgccgtgttgagcaagaaacagcttgtggagctttgt 900 aaagcgttgatacaaagtcggagaccattcttgtgggtgattacggataagtcgtacaga 960 aataaagaagatgagcaagagaaggaagaagattgcataagtagtttcagagaagagctc 1020 gatgagataggaatggtggtttcatggtgtgatcagtttagggttttgaatcatagatcg 1080 ataggttgtttcgtgacgcattgcgggtggaactctacgctggagagcttggtttcagga 1140 gttccggtggtggcgtttccgcaatggaatgatcagatgatgaacgcgaagcttttagaa 1200 gattgttggaaaacaggtgtaagagtgatggagaagaaggaagaagaaggagttgtggtg 1260 gtggatagtgaggagatacggcggtgcattgaggaagttatggaagacaaggcggaggag 1320 tttagaggaaatgccacgaggtggaaggatttagcggcggaggctgtgagagaaggaggc 1380 tcttcctttaatcatctcaaagcttttgtcgatgagcacatctag 1425 SEQIDNO:205 Arabidopsisthaliana MANNNSNSPTGPHFLFVTFPAQGHINPSLELAKRLAGTISGARVTFAASISAYNRRMFST 60 ENVPETLIFATYSDGHDDGFKSSAYSDKSRQDATGNFMSEMRRRGKETLTELIEDNRKQN 120 RPFTCVVYTILLTWVAELAREFHLPSALLWVQPVTVFSIFYHYFNGYEDAISEMANTPSS 180 SIKLPSLPLLTVRDIPSFIVSSNVYAFLLPAFREQIDSLKEEINPKILINTFQELEPEAM 240 SSVPDNFKIVPVGPLLTLRTDFSSRGEYIEWLDTKADSSVLYVSFGTLAVLSKKQLVELC 300 KALIQSRRPFLWVITDKSYRNKEDEQEKEEDCISSFREELDEIGMVVSWCDQFRVLNHRS 360 IGCFVTHCGWNSTLESLVSGVPVVAFPQWNDQMMNAKLLEDCWKTGVRVMEKKEEEGVVV 420 VDSEEIRRCIEEVMEDKAEEFRGNATRWKDLAAEAVREGGSSFNHLKAFVDEHI 474 SEQIDNO:206 Arabidopsisthaliana atgggaagtaatgagggtcaagaaacacatgtcctaatggtagcattagcattccaaggt 60 catctcaatccaatgctcaaattcgcaaaacatctcgcacgaaccaatctacacttcact 120 ctcgccaccactgagcaagcccgtgacctcctctcttccaccgctgacgaacctcataga 180 ccggtggacctcgctttcttctcagacggtctacctaaagacgatccaagagatcccgac 240 actctcgcaaagtcattgaaaaaagatggagccaagaacttgtcaaaaatcatcgaagaa 300 aagagatttgattgcatcatctctgtgccttttactccctgggttccagctgttgcagct 360 gcacataacattccttgtgcaatcctctggatccaagcttgtggagctttttctgtttat 420 taccgttattacatgaagacaaatcctttccccgaccttgaagatctgaatcaaacagtg 480 gagttaccagctttaccattgttggaagtccgagatctcccgtcattgatgttaccttct 540 caaggagctaatgtcaataccctaatggcggaatttgcagattgtttgaaagatgtgaaa 600 tgggttttggttaactcgttttacgaactcgaatcagagatcatcgagtctatgtctgat 660 ttaaaacctataatcccaattggtcctcttgtttctccattcctgttgggaaatgatgaa 720 gaaaaaaccctagatatgtggaaagttgatgattattgtatggagtggcttgacaagcaa 780 gctaggtcttcagttgtttacatatctttcggaagcatactcaaatcattggagaatcaa 840 gttgagaccatagcaacggcattaaaaaacagaggagttccatttctttgggtgatacgg 900 ccgaaggagaaaggcgaaaacgtccaggttttgcaggagatggttaaagaaggtaaaggg 960 gttgtaactgaatggggtcaacaagaaaagatattgagccacatggcgatttcttgcttc 1020 atcacgcattgtggatggaactcgacgatcgagacggtggtgactggtgttcccgtggtg 1080 gcgtatccgacttggatagatcagccgcttgatgcgagactgcttgtggatgtgtttgga 1140 atcggagtaaggatgaagaacgacgctatcgatggagagcttaaggttgcagaggtggag 1200 agatgcattgaggccgtgacagagggacctgccgccgcggatatgaggaggagagcgacg 1260 gagctgaagcacgccgcaagatcggcgatgtcacctggtggatcttccgctcagaattta 1320 gactcgttcattagtgatatcccaatcacttga 1353 SEQIDNO:207 Arabidopsisthaliana MGSNEGQETHVLMVALAFQGHLNPMLKFAKHLARTNLHFTLATTEQARDLLSSTADEPHR 60 PVDLAFFSDGLPKDDPRDPDTLAKSLKKDGAKNLSKIIEEKRFDCIISVPFTPWVPAVAA 120 AHNIPCAILWIQACGAFSVYYRYYMKTNPFPDLEDLNQTVELPALPLLEVRDLPSLMLPS 180 QGANVNTLMAEFADCLKDVKWVLVNSFYELESEIIESMSDLKPIIPIGPLVSPFLLGNDE 240 EKTLDMWKVDDYCMEWLDKQARSSVVYISFGSILKSLENQVETIATALKNRGVPFLWVIR 300 PKEKGENVQVLQEMVKEGKGVVTEWGQQEKILSHMAISCFITHCGWNSTIETVVTGVPVV 360 AYPTWIDQPLDARLLVDVFGIGVRMKNDAIDGELKVAEVERCIEAVTEGPAAADMRRRAT 420 ELKHAARSAMSPGGSSAQNLDSFISDIPIT 450 SEQIDNO:208 Catharanthusroseus atggttaatcagctccatattttcaacttcccattcatggcacagggccatatgttaccc 60 gccttagacatggccaatctattcacttctcgtggagtcaaagtaacattaatcacaacc 120 catcaacatgttcccatgtttacaaaatccatagaaaggagcagaaattctggatttgat 180 atatccattcaatccatcaaattcccagcttcagaagttggtttacctgaaggaatcgaa 240 agtctagatcaagtttcaggggacgacgaaatgcttcctaagttcatgagaggagttaat 300 ttactccaacaacctctcgaacaactattgcaagaatctcgtcctcattgtcttctttct 360 gatatgttcttcccttggactactgaatctgctgctaaatttggtattcccagattgctt 420 tttcatgggtcctgttcctttgccctctctgcagctgaaagtgtgagaagaaataaacct 480 ttcgagaatgtttccacagacacagaggaatttgttgtgcctgatcttccccaccaaatt 540 aaattaaccagaacacaaatttcaacatacgaaagggaaaatattgagtcagattttacc 600 aaaatgctgaagaaagttagggattcagaatccacatcttacggagttgtagtcaatagt 660 ttctatgaacttgaaccagattatgccgattattacatcaacgttttgggaagaaaagca 720 tggcatatagggccttttttgctttgtaacaaatcacgagctgaagataaagcccaaagg 780 gggaagaaatcagcaattgatgcagacgaatgtttaaattggcttgattcgaaacaacca 840 aattccgtaatttatctctgtttcggaagtatggccaatttaaattctgcccaattacac 900 gaaattgcaacagcccttgaatcctccggccaaaatttcatctgggttgttagaaaatgt 960 gtggacgaagaaaacagttcaaaatggtttccagaaggattcgaagaaagaacaaaagaa 1020 aaagggctaattataaagggatgggcaccacaaaccctaattcttgaacacgaatcagta 1080 ggagcatttgttacccattgtggttggaattcaactcttgaaggaatctgcgcaggggtt 1140 cctctggtgacttggcctttctttgctgagcaatttttcaatgagaaattgattacagag 1200 gtactgaaaacgggatacggagttggggctcggcaatggagtagagtttcaacagagatt 1260 ataaaaggagaagccatagctaatgctattaatcgagtaatggtgggtgatgaagctgtt 1320 gagatgagaaacagagcaaaagatttgaaggaaaaggcaagaaaagctttggaagaagat 1380 ggatcttcttatcgtgatcttactgctcttattgaagaattgggggcatatcgttctcaa 1440 gttgaaagaaagcaacaagactag 1464 SEQIDNO:209 Catharanthusroseus MVNQLHIFNFPFMAQGHMLPALDMANLFTSRGVKVTLITTHQHVPMFTKSIERSRNSGFD 60 ISIQSIKFPASEVGLPEGIESLDQVSGDDEMLPKFMRGVNLLQQPLEQLLQESRPHCLLS 120 DMFFPWTTESAAKFGIPRLLFHGSCSFALSAAESVRRNKPFENVSTDTEEFVVPDLPHQI 180 KLTRTQISTYERENIESDFTKMLKKVRDSESTSYGVVVNSFYELEPDYADYYINVLGRKA 240 WHIGPFLLCNKSRAEDKAQRGKKSAIDADECLNWLDSKQPNSVIYLCFGSMANLNSAQLH 300 EIATALESSGQNFIWVVRKCVDEENSSKWFPEGFEERTKEKGLIIKGWAPQTLILEHESV 360 GAFVTHCGWNSTLEGICAGVPLVTWPFFAEQFFNEKLITEVLKTGYGVGARQWSRVSTEI 420 IKGEAIANAINRVMVGDEAVEMRNRAKDLKEKARKALEEDGSSYRDLTALIEELGAYRSQ 480 VERKQQD 487 SEQIDNO:210 Solanumlycopersicum atgactactcacaaagctcattgcttaattttgccatttccaggccaaggtcatatcaac 60 ccaatgcttcaattctccaaacgtttacaatccaaacgcgttaaaatcactatagcactc 120 acaaaatcctgtttgaaaacaatgcaagaattgtcaacttcagtatcaatcgaggcgatt 180 tctgatggctacgatgatggtggtttccatcaagcagaaaatttcgtagcctacataaca 240 cgattcaaagaagttggttcggatactctgtctcagcttattaaaaaattggaaaatagt 300 gattgtcctgtaaattgcatagtatatgatccattcattccttgggctgttgaagttgca 360 aaacaatttggattaattagtgctgcatttttcacacaaaattgtgtagtggataatctt 420 tattaccatgtacataaaggggtgataaaacttccacctactcaaaatgacgaagaaata 480 ttaattcctggatttccaaattcgatcgatgcatcagatgtaccttcttttgttattagt 540 cctgaagcagaaaggatagttgaaatgttagcaaatcaattctcaaatcttgacaaagtt 600 gattatgttctaatcaatagcttctatgagttggagaaagaggtaaatgaatggatgtca 660 aagatatatccaataaagacaattggaccaacaataccatcaatgtacttagacaagaga 720 ctacatgatgataaagagtatggtcttagtgtcttcaagccaatgacaaatgaatgtcta 780 aattggttaaaccatcaaccaattagctcagtggtgtatgtatcatttggaagtataacc 840 aaattaggagatgagcaaatggaagaattggcatggggtttgaagaatagcaacaagagc 900 ttcttgtgggttgttaggtctactgaagagcccaaacttcccaacaactttattgaggaa 960 ttaacaagtgaaaaaggcttagtggtgtcatggtgtccacaattacaagtgttggaacat 1020 gaatcgacaggttgttttctgacgcactgtggatggaattcaactctggaagcgattagt 1080 ttgggagtgccaatggtggcaatgccacaatggtctgatcaaccaacaaatgcaaagctt 1140 gtgaaagatgtttgggaaataggtgttagagccaaacaagatgaaaaaggggtagttaga 1200 agagaagttatagaagaatgtataaagctagtgatggaagaagataaaggaaaactaatt 1260 agagaaaatgcaaagaaatggaaggaaatagctagaaatgttgtgaatgaaggaggaagt 1320 tcagataaaaacattgaagaatttgtttccaagttggttactatttcctaa 1371 SEQIDNO:211 Solanumlycopersicum MTTHKAHCLILPFPGQGHINPMLQFSKRLQSKRVKITIALTKSCLKTMQELSTSVSIEAI 60 SDGYDDGGFHQAENFVAYITRFKEVGSDTLSQLIKKLENSDCPVNCIVYDPFIPWAVEVA 120 KQFGLISAAFFTQNCVVDNLYYHVHKGVIKLPPTQNDEEILIPGFPNSIDASDVPSFVIS 180 PEAERIVEMLANQFSNLDKVDYVLINSFYELEKEVNEWMSKIYPIKTIGPTIPSMYLDKR 240 LHDDKEYGLSVFKPMTNECLNWLNHQPISSVVYVSFGSITKLGDEQMEELAWGLKNSNKS 300 FLWVVRSTEEPKLPNNFIEELTSEKGLVVSWCPQLQVLEHESTGCFLTHCGWNSTLEAIS 360 LGVPMVAMPQWSDQPTNAKLVKDVWEIGVRAKQDEKGVVRREVIEECIKLVMEEDKGKLI 420 RENAKKWKEIARNVVNEGGSSDKNIEEFVSKLVTIS 456 SEQIDNO:212 ArtificialSequence atggctaccagtgactccatagttgacgaccgtaagcagcttcatgttgcgacgttccca 60 tggcttgctttcggtcacatcctcccttaccttcagctttcgaaattgatagctgaaaag 120 ggtcacaaagtctcgtttctttctaccaccagaaacattcaacgtctctcttctcatatc 180 tcgccactcataaatgttgttcaactcacacttccacgtgtccaagagctgccggaggat 240 gcagaggcgaccactgacgtccaccctgaagatattccatatctcaagaaggcttctgat 300 ggtcttcaaccggaggtcacccggtttctagaacaacactctccggactggattatttat 360 gattatactcactactggttgccatccatcgcggctagcctcggtatctcacgagcccac 420 ttctccgtcaccactccatgggccattgcttatatgggaccctcagctgacgccatgata 480 aatggttcagatggtcgaaccacggttgaggatctcacgacaccgcccaagtggtttccc 540 tttccgaccaaagtatgctggcggaagcatgatcttgcccgactggtgccttacaaagct 600 ccggggatatctgatggataccgtatggggatggttcttaagggatctgattgtttgctt 660 tccaaatgttaccatgagtttggaactcaatggctacctcttttggagacactacaccaa 720 gtaccggtggttccggtgggattactgccaccggaaatacccggagacgagaaagatgaa 780 acatgggtgtcaatcaagaaatggctcgatggtaaacaaaaaggcagtgtggtgtacgtt 840 gcattaggaagcgaggctttggtgagccaaaccgaggttgttgagttagcattgggtctc 900 gagctttctgggttgccatttgtttgggcttatagaaaaccaaaaggtcccgcgaagtca 960 gactcggtggagttgccagacgggttcgtggaacgaactcgtgaccgtgggttggtctgg 1020 acgagttgggcacctcagttacgaatactgagccatgagtcggtttgtggtttcttgact 1080 cattgtggttctggatcaattgtggaagggctaatgtttggtcaccctctaatcatgcta 1140 ccgatttttggggaccaacctctgaatgctcgattactggaggacaaacaggtgggaatc 1200 gagataccaagaaatgaggaagatggttgcttgaccaaggagtcggttgctagatcactg 1260 aggtccgttgttgtggaaaaagaaggggagatctacaaggcgaacgcgagggagctgagt 1320 aaaatctataacgacactaaggttgaaaaagaatatgtaagccaattcgtagactatttg 1380 gaaaagaatgcgcgtgcggttgccatcgatcatgagagttaa 1422 SEQIDNO:213 Steviarebaudiana atggcggaacaacaaaagatcaagaaatcaccacacgttctactcatcccattcccttta 60 caaggccatataaaccctttcatccagtttggcaaacgattaatctccaaaggtgtcaaa 120 acaacacttgttaccaccatccacaccttaaactcaaccctaaaccacagtaacaccacc 180 accacctccatcgaaatccaagcaatttccgatggttgtgatgaaggcggttttatgagt 240 gcaggagaatcatatttggaaacattcaaacaagttgggtctaaatcactagctgactta 300 atcaagaagcttcaaagtgaaggaaccacaattgatgcaatcatttatgattctatgact 360 gaatgggttttagatgttgcaattgagtttggaatcgatggtggttcgtttttcactcaa 420 gcttgtgttgtaaacagcttatattatcatgttcataagggtttgatttctttgccattg 480 ggtgaaactgtttcggttcctggatttccagtgcttcaacggtgggagacaccgttaatt 540 ttgcagaatcatgagcaaatacagagcccttggtctcagatgttgtttggtcagtttgct 600 aatattgatcaagcacgttgggtcttcacaaatagtttttacaagctcgaggaagaggta 660 atagagtggacgagaaagatatggaacttgaaggtaatcgggccaacacttccatccatg 720 taccttgacaaacgacttgatgatgataaagataacggatttaatctctacaaagcaaac 780 catcatgagtgcatgaactggttagacgataagccaaaggaatcagttgtttacgtagca 840 tttggtagcctggtgaaacatggacccgaacaagtggaagaaatcacacgggctttaata 900 gatagtgatgtcaacttcttgtgggttatcaaacataaagaagagggaaagctcccagaa 960 aatctttcggaagtaataaaaaccggaaagggtttgattgtagcatggtgcaaacaattg 1020 gatgtgttagcacacgaatcagtaggatgctttgttacacattgtgggttcaactcaact 1080 cttgaagcaataagtcttggagtccccgttgttgcaatgcctcaattttcggatcaaact 1140 acaaatgccaagcttctagatgaaattttgggtgttggagttagagttaaggctgatgag 1200 aatgggatagtgagaagaggaaatcttgcgtcatgtattaagatgattatggaggaggaa 1260 agaggagtaataatccgaaagaatgcggtaaaatggaaggatttggctaaagtagccgtt 1320 catgaaggtggtagctcagacaatgatattgtcgaatttgtaagtgagctaattaaggct 1380 taa 1383