PHARMACEUTICAL FORMULATIONS CONTAINING IPIDACRINE AND THEIR USE FOR THE TREATMENT OF DISORDERS OF POTENCY AND DISORDERS OF OTHER FORMS OF SEXUAL ACTIVITY

Abstract

The invention is based on the use of ipidacrine to treat potency disorders and can be used in clinics for treating potency disorders related to the reduced production of reproductive gland hormones, disorders caused by chronic, including physical, stress, and also associated with spontaneously reduced sexual function, including anorgasmia or delayed ejaculation, and other sexual activity disorders not limiting the scope of the invention.

Claims

1. (canceled)

2. A method of treating potency disorders in a male subject with ipidacrine as a drug for the treatment, where the potency disorders are manifested against the background of spontaneously reduced sexual function, including manifested anorgasmia or delayed ejaculation.

3-5. (canceled)

6. The method of claim 2, further comprising: administering to said male subject a therapeutically effective amount of a pharmaceutical composition comprising ipidacrine to reverse or lessen the reduction of sexual function; and reversing or lessening the spontaneously reduced sexual function in accordance with the administering.

7. The method of claim 6, wherein the pharmaceutical composition is administered at an ipidacrine dosage of 3-300 mg.

8. The method of claim 7, wherein the pharmaceutical composition is administered at an ipidacrine dosage of 60 mg.

9. The method of claim 2, wherein the spontaneously reduced sexual function is manifested anorgasmia.

10. The method of claim 6, wherein the spontaneously reduced sexual function is manifested anorgasmia.

11. The method of claim 7, wherein the spontaneously reduced sexual function is manifested anorgasmia.

12. The method of claim 8, wherein the spontaneously reduced sexual function is manifested anorgasmia.

13. The method of claim 2, wherein the spontaneously reduced sexual function is delayed ejaculation.

14. The method of claim 6, wherein the spontaneously reduced sexual function is delayed ejaculation.

15. The method of claim 7, wherein the spontaneously reduced sexual function is delayed ejaculation.

16. The method of claim 8, wherein the spontaneously reduced sexual function is delayed ejaculation.

Description

BRIEF DESCRIPTION OF DRAWINGS

[0013] FIG. 1 shows the effect of the drugs to be compared on the sexual activity (mean number of ejaculations) of hemigonadectomized rats when administering the drugs in mean therapeutic doses in the course of treatment.

[0014] FIG. 2 presents the effect of ipidacrine on the sexual activity of male rats using the model of chronic stress caused by electrical current exposure.

[0015] FIG. 3 shows the effect of ipidacrine on the sexual activity of rats using the model of spontaneous sexual dysfunction (by criterion of the number of ejaculations)

DESCRIPTION OF THE PREFERRED EMBODIMENTS

Definitions

[0016] The term pharmaceutical formulation means a formulation in tablet or capsule form (as an example but not limited to hard gelatin capsules), including that of prolonged action, containing ipidacrine as a main biologically active substance in an effective amount from 3 to 300 mg per dose and additionally containing the pharmaceutically acceptable excipients.

[0017] The term pharmaceutically acceptable excipient means a substance needed to improve tableting, such as, but not limited to, hydroxypropyl methylcellulose, microcrystalline cellulose, colloidal silicon dioxide, magnesium stearate and other conventional pharmaceutical excipients which do not limit the scope of the invention.

EMBODIMENTS OF INVENTION

[0018] To illustrate the effects of pharmaceutical formulations based on ipidacrine, here in are the following examples which do not limit the scope of the invention.

Example 1: Preparation of Pharmaceutical Formulations Containing Ipidacrine

[0019] The pharmaceutical tableted formulations (including those of prolonged action) on the basis of ipidacrine are made in a standard way of direct compression. All the ingredients (except magnesium stearate) are stirred to obtain a homogeneous powder mixture using a Y-shaped mixer or similar equipment. Then magnesium stearate is added, and the resulting mixture is stirred for 2 minutes. The obtained tablet mass is subjected to a tableting process (tablet diameter of 10 mm or 11 mm in accordance with industry standard OST 64-072-89, with a scoreline and bevel edge) at a pressing force of 9-10 kN.

[0020] Pharmaceutical formulations in capsules are made using standard processing methods by mixing active ingredients and excipients in the correct proportion and further encapsulation.

[0021] Pharmaceutical Formulation 1 (Content for 1 Tablet of 100 mg):

[0022] Active Substance:

[0023] Ipidacrine 3 mg

[0024] Pharmaceutically Acceptable Excipients:

[0025] hydroxypropyl methylcellulose 35 mg

[0026] microcrystalline cellulose 60 mg

[0027] colloidal silicon dioxide 1 mg

[0028] magnesium stearate 1 mg

[0029] Pharmaceutical Formulation 2 (Content for 1 Tablet of 250 mg):

[0030] Active Substance:

[0031] Ipidacrine 40 mg

[0032] Pharmaceutically Acceptable Excipients:

[0033] hydroxypropyl methylcellulose 75 mg

[0034] microcrystalline cellulose 128.74 mg

[0035] colloidal silicon dioxide 3.13 mg

[0036] magnesium stearate 3.13 mg

[0037] Pharmaceutical Formulation 3 (Content for 1 Tablet of 600 mg):

[0038] Active Substance:

[0039] Ipidacrine 150 mg

[0040] Pharmaceutically Acceptable Excipients:

[0041] hydroxypropyl methylcellulose 188 mg

[0042] microcrystalline cellulose 250 mg

[0043] colloidal silicon dioxide 6 mg

[0044] magnesium stearate 6 mg

[0045] Pharmaceutical Formulation 3 (Content for 1 Tablet of 1000 mg):

[0046] Active Substance:

[0047] Ipidacrine 300 mg

[0048] Pharmaceutically Acceptable Excipients:

[0049] hydroxypropyl methylcellulose 280 mg

[0050] microcrystalline cellulose 400 mg

[0051] colloidal silicon dioxide 10 mg

[0052] magnesium stearate 10 mg

[0053] Pharmaceutical Formulation 4 (Content for 1 Tablet of 230 mg):

[0054] Active Substance:

[0055] Ipidacrine 40 mg

[0056] Pharmaceutically Acceptable Excipients:

[0057] microcrystalline cellulose 140 mg

[0058] colloidal silicon dioxide 2 mg

[0059] magnesium stearate 2 mg

[0060] Pharmaceutical Formulation 6 (Content for 1 Prolonged Action Tablet of 600 mg)

[0061] Active Substance:

[0062] Ipidacrine 60 mg

[0063] Pharmaceutically Acceptable Excipients:

[0064] microcrystalline cellulose 216 mg

[0065] colloidal silicon dioxide 2 mg

[0066] hydroxypropyl methylcellulose 120 mg

[0067] magnesium stearate 2 mg

Example 2. Evaluation of the Effects of Ipidacrine Pharmaceutical Formulation in Rats with the Reduced Sexual Activity Caused by Hemigonadectomy

[0068] The hemigonadectomy is similar to the pathological processes observed in the clinical picture and associated with the decrease in hormone production by the sex glands.

[0069] The model of hypogonadal state in rats induced by the hemigonadectomy corresponds to the pathological processes observed in the clinical picture and associated with the decreased hormone production of the sex glands.

[0070] The study of the ipidacrine pharmacological activity was conducted in albino male rats. The following six groups of animals were formed: 1intact, 2treated with sildenafil, 3galantamine, 4ipidacrine, 5gonadectomized, 6control.

[0071] 30 days before the start of the main study, the experiment was performed (3 times a week for 2 weeks), namely introduction of a receptive female to the male rat. The number of ejaculations was recorded. Those animals whose level of sexual activity was characterized by stable performance of 1-2 ejaculations during the period of the test were selected.

[0072] The selected male rats underwent the hemigonadectomy (at the right) performed under ether anesthesia. Then, during 7 and 14 days prior to the course administration of the drugs, daily testing of sexual activity of the hemigonadectomized animals was carried out. The latency period and the number of mounts, intromissions and ejaculations in different groups of the animals were considered.

[0073] Administration of ipidacrine at a dose of 1.7 mg/kg once daily for 7 days to hemigonadectomized rats eliminates fluctuations in the level of the sexual activity due to external stressor effects throughout the whole course of the therapy and contributes to the increase of central motivation and ejaculatory components. Ipidacrine in the course therapy of 2 times a day for 14 days in hemigonadectomized rats at a dose range of 0.85 to 5.1 mg/kg produces a dose-dependent increase in the sexual activity of the animals, with a maximum manifestation in 3 to 5 days at a dose of 5.1 mg/kg, and in 11 to 14 days at a dose of 0.85 to 1.7 mg/kg (FIG. 1).

Example 3. Evaluation of the Effects of an Ipidacrine Pharmaceutical Formulation at Reduced Sexual Activity in Rats Caused by Physical Stress

[0074] The most appropriate model (in terms of the ease of implementation) is the model of psychogenic sexual dysfunction caused by exposure to physical factors (electrical current) in rats.

[0075] The pre-experiment with the animals was performed similar to that described in Example 2.

[0076] The two groups of the animals similar in the sexual activity level were formed: control (administration of distilled water), pharmaceutical formulation (on ipidacrine basis at a dose of 1.7 mg/kg). The animals of the control and experimental groups were daily exposed to current with a voltage of 30V for 30 min once a minute with an impulse with duration of 1 sec. The drug was administered 30 minutes before the stress, the testing was performed 60 min after the stress.

[0077] The administration of ipidacrine at a dose of 1.7 mg/kg once a day prior the stress contributed to a slower formation of sexual dysfunction in the rats. In an initial period (1 to 3 days), the parameters of activity were comparable to the parameters in the group nonsusceptible to the stress. Starting from the 5.sup.th day, a significant increase in the latency period of mounts and intromissions compared to the animals nonsusceptible to stress (in the group without treatment, such changes were observed from the 1.sup.st day of the experiment) was registered. Significant differences from the group without treatment on a number of indicators were observed on the 7.sup.th day (latency period of intromissions) and on the 10.sup.th day (latency period of intromissions and mounts).

[0078] When comparing the sexual activity of the stressed animals without treatment and against the background of the ipidacrine therapy at a dose of 1.7 mg/kg, the period from the 3.sup.rd to 10.sup.th day of the experiment was the most significant. The administration of ipidacrine provided the maintenance of the frequency of ejaculations at a level of 60-100%, while in the comparison group, this figure reduced to 20%. In the same period, a higher rate of renewal of activity after the first ejaculation was recorded in the animals of the ipidacrine group. By the end of the 14 day exposure to stress, the number of ejaculations remained at a level of 0.4-0.6 (on the 10.sup.th and 14.sup.th day) in the animals of the treatment group, while in the group without treatment, this parameter was not greater than 0.2 (7.sup.th and 14.sup.th day) (FIG. 2).

Example 4. The Evaluation of Effects of Ipidacrine Pharmaceutical Formulation on a Model of Initially Hypoactive Male Rats with Spontaneously Reduced Sexual Function

[0079] This model is associated with the clinical conditions of anorgasmia or delayed ejaculation in humans. According to the results of the five-fold testing of the intact animals, the groups of hypoactive male rats were formed. The criterion for selection was average number of ejaculations of less than 0.5 according to the results of the five-fold testing.

[0080] To assess the effect of ipidacrine on sexual function of rats with the reduced sexual activity, the groups of the animals with initially low manifestations of copulatory and ejaculatory components of behavior were formed. This model reflects the clinical conditions of anorgasmia and delayed ejaculation. The effect of the drug on the integral indicator of the sexual function (number of ejaculations) at a dose of 0.85 to 5.1 mg/kg when administered daily is shown in FIG. 3

[0081] In hypoactive animals undergoing daily course therapy with the pharmaceutical composition (ipidacrine content of 0.85 mg/kg), an increase in the sexual activity (starting with the 2.sup.nd week) was registered. It was manifested in the reduction of the latency period of mounts and intromissions (significant differences from the background were registered on the 7.sup.th to 14.sup.th day of observation). These data allow us to characterize the effect of the drug (0.85 mg/kg) on the trend level as similar changes of indicators were observed in the control group. An increase in the average number of ejaculations in the group up to 0.6-0.8 and increase of the rate of occurrence of ejaculations to 80% were noted. In some animals, the renewal of sexual activity was observed after the first ejaculation (on the average in 4-6 minutes) in contrast to the control group. While administering the pharmaceutical formulation with ipidacrine at a dose of 1.7 mg/kg (considering interspecies dose conversion), an increased central motivational component (2 to 4-fold reduction in the latency time of mounts and intromissions) after the first administration of the drug was revealed. In the subsequent periods, a slight decrease in the sexual activity (on the 3.sup.rd or 5.sup.th day) with an increase of indicators of sexual behavior on the 7.sup.th, 10.sup.th and 14.sup.th day of the administration of the drug was observed. This was accompanied by an increase of the ejaculatory component in comparison with the control throughout the course of administration of the drug in the test dose. The number of animals able to ejaculate also increased (up to 80%).

[0082] The administration of ipidacrine at a higher dose (5.1 mg/kg) increased the sexual activity in the animals during the first 5 days of observation, which was manifested by an increase in central motivational and ejaculatory components. The latency period of mounts reduced from 33.05.35 to 9.31.93 s (p<0.05), and the latency period of intromissions from 59.811.34 to 10.51.76 (p<0.05) in 3 days of observation. Subsequent administration of the drug at this dose produced a negative effect on the manifestation of the sexual activity while in 10, 14 days of the experiment the values of indicators were comparable to the control.

[0083] Therefore, the administration of ipidacrine in the animals with the spontaneous sexual hypoactivity leads to an increase of copulatory and ejaculatory components when administered at doses of 1.7 and 5.1 mg/kg. The drug at a dose of 5.1 mg/kg is effective only when administered for 5 days. Ipidacrine at a dose of 1.7 mg/kg showed an activating effect on the sexual function throughout the whole observation period (14 days).

REFERENCES

[0084] Clemens L G, Barr P, Dohanich G P. Cholinergic regulation of female sexual behavior in rats demonstrated by manipulation of endogenous acetylcholine. Physiol Behav. 1989 February; 45(2):437-42 [0085] Dohanich G P, McMullan D M, Brazier M M. Cholinergic regulation of sexual behavior in female hamsters. Physiol Behav. 1990 January; 47(1):127-31. [0086] Menard C S, Dohanich G P. Estrogen dependence of cholinergic systems that regulate lordosis in cycling female rats. Pharmacol Biochem Behav. 1994 June; 48(2):417-21. [0087] Clemens L G, Humphrys R R, Dohanich G P. Cholinergic brain mechanisms and the hormonal regulation of female sexual behavior in the rat. Pharmacol Biochem Behav. 1980 July; 13(1):81-8 [0088] Dohanich G P, Clemens L G. Brain areas implicated in cholinergic regulation of sexual behavior. Horm Behav. 1981 June; 15(2):157-67. [0089] Mitsushima D. Sex steroids and acetylcholine release in the hippocampus. Vitam Horm. 2010; 82:263-77 [0090] OCT64-072-89. . . [0091] Bianchetti A, Trabucchi M, Cipriani G. Aggressive behaviour associated with donepezil treatment: a case report. Int J Geriatr Psychiatry. 2003 July; 18(7):657-8. [0092] Bouman W P, Pinner G. Violent behavior-associated with donepezil. Am J Psychiatry. 1998 November; 155(11):1626-7. [0093] Lo Coco D, Cannizzaro E. Inappropriate sexual behaviors associated with donepezil treatment: a case report. J Clin Psychopharmacol. 2010 April; 30(2):221-2. [0094] Kojima J, Onodera K, Ozeki M, Nakayama M. Ipidacrine (NIK-247): A Review of Multiple Mechanisms as an Antidementia Agent CNS Drug Reviews 1998, Vol. 4, No. 3, pp. 247.259