Laundry wastewater biological treatment preparation, and device and method for treating laundry wastewater by using same
10259733 ยท 2019-04-16
Assignee
Inventors
- Yuuji Nakajima (Tokyo, JP)
- Naoki Ogawa (Tokyo, JP)
- Kyoko Wada (Tokyo, JP)
- Yuta Nakatsuchi (Tokyo, JP)
- Atsushi Kinugasa (Tokyo, JP)
Cpc classification
C02F2307/12
CHEMISTRY; METALLURGY
C02F2209/005
CHEMISTRY; METALLURGY
C02F2209/008
CHEMISTRY; METALLURGY
C02F3/348
CHEMISTRY; METALLURGY
Y02W10/10
GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
C02F2209/08
CHEMISTRY; METALLURGY
C02F2103/002
CHEMISTRY; METALLURGY
International classification
Abstract
The present invention includes: a laundry wastewater tank that temporarily stores laundry wastewater from a plant facility; a biological treatment tank in which the laundry wastewater from the laundry wastewater tank is aerated and mixed with activated sludge; a treated water tank that stores the treated laundry wastewater that has been biologically treated; and a treatment preparation supplying device that supplies a laundry wastewater biological treatment preparation to the biological treatment tank. The laundry wastewater biological treatment preparation is introduced when a change in water quality that deteriorates the biological treatment in the biological treatment tank is detected. Thus, biological treatment can be performed stably.
Claims
1. A device for treating laundry wastewater, comprising: a laundry wastewater tank that temporarily stores laundry wastewater from a plant facility; a biological treatment tank in which laundry wastewater from the laundry wastewater tank is aerated and mixed with activated sludge and is biologically treated; a treated water tank that stores treated laundry wastewater that has been biologically treated; a supplying device that supplies a laundry wastewater biological treatment preparation to the biological treatment tank; a first COD measurement device that measures a COD of the laundry wastewater in the laundry wastewater tank; a second COD measurement device that measures a COD of the treated laundry wastewater in the treated water tank; a real-time PCR analyzer that analyzes a status of bacterial flora in the biological treatment tank; and a control device that introduces the laundry wastewater biological treatment preparation into the biological treatment tank from the supplying device based on the measurement results by the first COD measurement device and the second COD measurement device and the analysis results by the real-time PCR analyzer.
2. The device for treating laundry wastewater according to claim 1, further comprising: means for extracting activated sludge from the biological treatment tank; and a freeze-drying device that freeze-dries the extracted activated sludge and thereby produces a laundry wastewater biological treatment preparation.
3. The device for treating laundry wastewater according to claim 2, wherein the laundry wastewater biological treatment preparation produced in the freeze-drying device is supplied to the supplying device.
4. A method for treating laundry wastewater, the method comprising measuring a COD of laundry wastewater in a laundry wastewater tank and treated laundry wastewater in a treated water tank and analyzing a status of bacterial flora in a biological treatment tank, using a first COD measurement device, a second COD measurement device and a real-time PCR analyzer, introducing a laundry wastewater biological treatment preparation into the biological treatment tank from a supplying device using a control device based on the measurement results and the analysis results thereof.
5. The method for treating laundry wastewater according to claim 4, wherein the laundry wastewater biological treatment preparation includes the genus Sphingomonas and related genera thereof, and a compositional ratio of the genus Sphingomonas and related genera thereof in the biological treatment tank is analyzed using the real-time PCR analyzer.
Description
BRIEF DESCRIPTION OF DRAWINGS
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DESCRIPTION OF EMBODIMENTS
(19) Hereinafter, suitable embodiments of the invention will be described in detail with reference to the attached drawings. The invention is not intended to be limited to these embodiments, and in a case in which there are a number of embodiments, the invention also includes a configuration of combining various embodiments.
Example 1
(20)
(21) As illustrated in
(22) In the present example, a first COD measurement device 19-1 that measures the COD in the laundry wastewater tank 12 in order to detect a change in water quality that deteriorates the biological treatment, and a control device 17 that introduces a laundry wastewater biological treatment preparation 21 into the biological treatment tank 14, based on the measurement results of the first COD measurement device 19-1, are provided.
(23) Furthermore, at the initiation of the biological treatment tank 14, a laundry wastewater biological treatment preparation 21 is introduced so as to accelerate acclimatization of activated sludge in the biological treatment tank.
(24) Here, the laundry wastewater biological treatment preparation 21 of the present example is a laundry wastewater biological treatment preparation that is added to the sludge of the biological treatment tank 14 for treating laundry wastewater 11 that has been used to wash used clothing of plant workers or used cloth. The laundry wastewater biological treatment preparation 21 is a freeze-dried sludge that is formed by freeze-drying a laundry wastewater-acclimatized activated sludge 13 that has been acclimatized using laundry wastewater 11.
(25) It is particularly preferable that this freeze-dried sludge includes the genus Sphingomonas and related genera thereof, all of which have sphingolipids in the surface layer of the bacteria.
(26) Examples of this genus Sphingomonas and related genera thereof include, but are not limited to, Sphingomonas, Novosphingobium, Sphingobium, and Sphingopyxis.
(27) Furthermore, when the freeze-drying treatment is performed, a protective agent may be added so as to attempt protection of the freeze-dried sludge. Here, examples of the protective agent include, for example, glycerin, dimethyl sulfoxide, sodium glutamate, starch, and skimmed milk.
(28) The freeze-drying treatment may be carried out by any known technique, and an example thereof will be described here.
(29) First, a laundry wastewater-acclimatized activated sludge 13 is collected and centrifuged using a centrifuge device (for example, 3,000 rpm for 10 minutes).
(30) Next, a precipitate is weighed and transferred to a container for freeze-drying.
(31) Thereafter, the precipitate is subjected to a freeze-drying treatment overnight at 20 C.
(32) In a case in which a protective agent is used, a predetermined amount (for example, 1% to 10%) of the protective agent is introduced into the activated sludge when the activated sludge is transferred to the container for freeze-drying.
(33) Photographs of a freeze-dried product are shown in
(34)
(35) These freeze-dried sludges that had been subjected to a freeze-drying treatment were both easily dispersible in water.
(36)
(37)
(38) Thereby, a freeze-dried sludge to which a protective agent had not been added, exhibited satisfactory settleability of the freeze-dried sludge after completion of acclimatization, compared to the settleability before the initiation of acclimatization.
(39) As a result, since the sludge is not suspended, for example, in a case in which the activated sludge is introduced into a separation membrane, clogging of the separation membrane does not occur, which is preferable.
(40) A wastewater treatment test was performed using this freeze-dried sludge.
(41)
(42) As illustrated in
(43) Here, simulated laundry wastewater 11 was operated at pH 6.5 to 8.0 and at 25 C.
(44) Regarding the simulated laundry wastewater, a mixture of NH.sub.4Cl (N component), KH.sub.2PO.sub.4 (P component), and a detergent (including cationic and nonionic surfactants) was used.
(45)
(46) As in the case of related art, in a case in which sewage sludge is used and acclimatized, foaming occurs. Therefore, laundry wastewater with 180 ppm COD-Mn was used, and this was supplied intermittently. This operation was continued until foaming stopped.
(47) In the present test, foaming continued for 12 days, and the initiation of acclimatization was on the 12.sup.th day.
(48) Therefore, laundry wastewater with 180 ppm COD-Mn could be continuously supplied from the 12.sup.th day.
(49) Also, since the COD concentration of the treated laundry wastewater 15 was below 20 ppm COD-Mn on the 17.sup.th day, the laundry wastewater was supplied after raising the concentration of the supplied laundry wastewater 11 to 240 ppm COD-Mn.
(50) However, as a result of the increase in the COD concentration, the COD concentration of the treated laundry wastewater 15 did not go below 20 ppm COD-Mn until 23 days elapsed.
(51) On the contrary, in a case in which the freeze-dried sludge of the present example is used and acclimatized, shortening of the acclimatization time could be attempted.
(52)
(53) In a case in which freeze-dried sludge was used and acclimatized, in a case in which laundry wastewater with 180 ppm COD-Mn was used, the COD concentration of the treated laundry wastewater 15 could reach 20 ppm COD-Mn on the first day.
(54) Therefore, the laundry wastewater 11 that was subsequently supplied was supplied after raising the concentration of the laundry wastewater 11 to 240 ppm COD-Mn. Even in this case, a COD concentration of 20 ppm COD-Mn of the treated laundry wastewater 15 could be continuously achieved, and therefore, the laundry wastewater 11 that was supplied on the 8.sup.th day was supplied after increasing the COD concentration of the laundry wastewater 11 to 300 ppm COD-Mn.
(55) When it is said that this COD concentration is 300 ppm COD-Mn, this is a load concentration for actual machine application. The COD concentration of the treated laundry wastewater 15 could reach 20 ppm COD-Mn on the 14.sup.th day.
(56) As a result, in a case in which freeze-dried sludge is supplied to the activated sludge tank, COD decomposition performance can be immediately expected.
(57) Therefore, it was found that performing an acclimatization treatment for a long time period as in the case of performing an acclimatization treatment using seed sludge as in conventional cases, is unnecessary.
(58) Furthermore, conventionally, in a case in which the COD concentration of laundry wastewater is, for example, above 300 ppm COD-Mn, it has been necessary to subject the laundry wastewater to a dilution treatment using a diluent before the laundry wastewater is introduced into a biological treatment tank. However, now, a buffer tank in which treatment water for such a dilution treatment is stored, or a dilution treatment is carried out, may be reduced, or even installation of a buffer tank becomes unnecessary.
(59) Next, the composition ratios of principal microorganisms in the case of a conventional seed sludge treatment and in the case of using the freeze-dried sludge of the present example, in a case in which simulated laundry wastewater was used, were checked.
(60)
(61) Here, the microorganisms to which attention was paid were the genus Sphingomonas and related genera thereof (Novosphingobium, Sphingobium, Sphingopyxis, and the like), and these will be described as principal microorganisms hereinafter.
(62) In a preliminary acclimatization in which foaming occurred, the composition ratio of the principal microorganisms was several percent (%).
(63) Thereafter, in a case in which main acclimatization was carried out, the composition ratio of the principal microorganisms increased to 5%, and in a case in which the COD concentration was increased to 240 ppm COD-Mn, the composition ratio of the principal microorganisms was only about 12%.
(64) Furthermore, the conformity ratio (symbol in the diagram) of the treated wastewater was also decreased, after the COD concentration was raised to 240 ppm COD-Mn in the main acclimatization.
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(66) Then, it was confirmed that the wastewater treatment capacity conformed to the input COD concentration from the 14.sup.th day.
(67)
(68) As illustrated in
(69) Here, in
(70) In
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(72) Table 1 shows the detection primers for the genus Sphingomonas and related genera thereof (Novosphingobium, Sphingobium, and Sphingopyxis).
(73) Table 2 shows forward primers and reverse primers of the detection primers (SPG5 to SPG9).
(74) Table 3 shows a gene sequence that was obtained by obtaining consensus sequences (results of automatic production of consensus sequences using BIOEDIT), which were obtained from 16S rDNA gene alignment analysis of the microorganisms of the genus Sphingomonas and related genera, subsequently comparing these consensus sequences with the alignment results, and deleting the portions that needed to be deleted for having very low commonality.
(75) A PCR primer for detection using these gene sequences (SPG7: underlined) are shown below.
(76) TABLE-US-00001 TABLE 1 Primer Determination criteria corresponding Evaluation of PCR product Reaction with name PCR product length Escherichia coli Univ. B present SPG1 C B B 107 absent SPG5 B B B 205 absent SPG6 B B B 207 absent SPG7 A B B 292 absent SPG8 B B B 211 absent SPG9 C B B 87 absent
(77) TABLE-US-00002 TABLE2 Primer Sequence corresponding No. Primer name SEQ.ID F1 GTGCGTAACGCGTGGGA SPG5 NO:1 SEQ.ID R1 GTGTCTCAGTCCCAGTGTGG NO:2 SEQ.ID F2 GGTGCGTAACGCGTGGG SPG6 NO:3 SEQ.ID R2 CGTGTCTCAGTCCCAGTGTG NO:4 SEQ.ID F3 GGGTGCGTAACGCGTGG SPG7 NO:5 SEQ.ID R3 TTCATCACTCACGCGGCATT NO:6 SEQ.ID F4 GCGCACGGGTGCGTAA SPG8 NO:7 SEQ.ID R4 GTCTCAGTCCCAGTGTGGC NO:8 SEQ.ID F5 TGGCTCAGAACGAACGCTG SPG9 NO:9 SEQ.ID R5 CGCGTTACGCACCCGT NO:10 (Base sequence 5 .fwdarw. 3)
(78) TABLE-US-00003 TABLE3 AGAGTTTGATCHTTGGCGTCAGAACGAACGCTGGCGGCATGCCTAAYAC ATGCAAGTCGAACCADNHCTTCGGDBBTAGTGGCGCAC AATCTRCCCTTKGSTDCGGAATAACHNNBDG AAAYBDVWGCTAATACCGNATDATGWCGTHVGHCCAAAGATTTATCGSC MAVGGATGAGCCCGCGTARGATTAGVTAGTTGGTGRGGTAAAVGCBHAC CAAGSCKACGATCYTTAGCTGGTCTGAGAGGATGATCACCCACACTGGG ACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTDGGGAATATTG GACAATGGGCGVAAGCCTGATCCAGC
GCCYTNGGTHGTAAAGCTCTTTTACCNGDGATGATAATGACATACBGGA GAATAAGCYCCGCTAACTYCGTGGCAGCVGCCGCGGTAATT
(79) Lanes 1 to 7 show the cases in which laundry wastewater sludge was used, and Lanes 8 to 14 show the cases in which Escherichia coli was used. Lane 15 has water only.
(80) A universal primer was used in Lane 1; SPG1 primer was used in Lane 2; SPG5 primer was in Lane 3; SPG6 primer was used in Lane 4; SPG7 primer was used in Lane 5; SPG8 primer was used in Lane 6; and SPG9 primer was used in Lane 7. Furthermore, a universal primer was used in Lane 8; SPG1 primer was used in Lane 9; SPG5 primer was used in Lane 10; SPG6 primer was used in Lane 11; SPG7 primer was used in Lane 12; SPG8 primer was used in Lane 13; SPG9 primer was used in Lane 14; and a universal primer was used in Lane 15.
(81)
(82) A DNA amplification test was carried out in a real-time PCR device, using a universal primer that can amplify DNA in all kinds of microorganisms, and a specific primer that can amplify DNA in specific microorganisms (genus Sphingomonas, genus Novosphingobium, genus Sphingobium, and genus Sphingopyxis) only (SPG7 of Lane 5).
(83) As shown in
(84) The calculation is based on the following:
Existence ratio (%)=DNA of specific microorganisms (Fragment I)/(DNA of all microorganisms (Fragment II)+1.4)100=46.6%
(85) Therefore, a bacterial flora analysis of the biological treatment tank 14 can be rapidly carried out using this real-time PCR device.
(86) From the above results, in the present example, the treatment process in the biological treatment tank is not carried out such that activated sludge is acclimatized for a long time period using conventional seed sludge, but acclimatization can be carried out rapidly using freeze-dried sludge.
(87) Regarding the case of applying this laundry wastewater biological treatment preparation 21 of freeze-dried sludge, the laundry wastewater biological treatment preparation 21 can be used at the time of starting up the biological treatment tank 14 prior to a wastewater treatment, instead of supplying conventional seed sludge.
(88) Furthermore, during the operation, the treatment can be carried out while checking the COD concentration of the laundry wastewater 11.
(89) In a case in which a plant has been stopped for a long time period, since the bacterial flora in the biological treatment tank 14 undergoes a change, at the time of start-up after this stoppage, the laundry wastewater biological treatment preparation 21 of the present example may be added to the biological treatment tank 14.
(90) At this time, the laundry wastewater biological treatment preparation 21 may be supplied while inspecting the status of bacterial flora, by checking the bacterial flora in the biological treatment tank 14 using a bacterial flora device such as a real-time PCR device. The bacterial flora device has been explained using a real-time PCR device; however, the invention is not intended to be limited to this, and any known device that can analyze the status of bacterial flora can all be applied.
(91) Furthermore, even in a case in which aeration has been brought to a stop due to power failure or the like, the situation can be rapidly coped with by adding the laundry wastewater biological treatment preparation 21 to the biological treatment tank 14.
(92) As such, according to the present example, for example, in a case in which laundry wastewater that has been used for washing, for example, working clothes or used cloth in a nuclear facility or a general plant facility, is subjected to decomposition, a biological treatment can be stably carried out by introducing a laundry wastewater biological treatment preparation, when a change in water quality that deteriorates the biological treatment in a biological treatment tank of activated sludge is detected.
Example 2
(93)
(94) As illustrated in
(95) As a result, conventionally, in a case in which the COD in the treated laundry wastewater 15 is higher than the standard value for discharged water 18 (for example, 20 ppm COD-Mn), there has been a need to separately dilute the laundry wastewater 11 that is supplied to the biological treatment tank 14. However, in the present example, since the situation can be coped with by supplying the laundry wastewater biological treatment preparation 21 to the biological treatment tank 14, such a dilution treatment as in conventional cases may be reduced, or may become unnecessary, and thus, reduction of the cost of initially facilities installation of plant and running equipment can be promoted.
Example 3
(96)
(97) As illustrated in
(98) As a result, the problem of operating the device by the course of nature as in conventional cases is solved, and stable operation of a biological treatment plant is enabled.
Example 4
(99)
(100) As illustrated in
(101) Thereby, the laundry wastewater biological treatment preparation 21 can be produced within a biological treatment plant facility, and if needed, the laundry wastewater biological treatment preparation 21 can be quickly supplied from a treatment preparation supplying device 22.
(102) As a result, it is not necessary to stock a large amount of the laundry wastewater biological treatment preparation 21, and constantly stable operation of biological treatment plants is enabled.
Example 5
(103)
(104) As illustrated in
(105) The laundry wastewater biological treatment preparation 21 obtainable by this freeze-drying device 32 can be directly applied to similar laundry wastewater treatment biological treatment devices. Therefore, the preparation can be supplied to, for example, wastewater treatment plants in the outside, where a biological treatment is performed on laundry wastewater in which similar detergents (including cationic and nonionic surfactants) were used.
(106) For example, in a case in which there is a demand for an urgent increase in the biological treatment performance (for example, stoppage of aeration in a biological treatment tank caused by power failure or the like), a technique of transporting activated sludge in the form of sludge by means of a vacuum truck or the like has been conventionally adopted. However, this transportation in the form of sludge has problems that 1) the personnel expenses and the cost for vehicle rental and the like increase; 2) during the summer season or the like, a chilling means and the like are needed at the time of conveyance, and in the case of long-distance transportation, there is also a need for an aeration treatment to be appropriately carried out, so that there is a high risk of deteriorating the sludge quality.
(107) In this regard, since the laundry wastewater biological treatment preparation 21 obtainable by the freeze-drying device 32 has a reduced volume, and storage and transportation of the preparation are easily achieved, sludge can be supplied to other wastewater treatment plants where biological treatments are performed on wastewater in which similar detergents (including cationic and nonionic surfactants) have been used, at low cost with low risk.
REFERENCE SIGNS LIST
(108) 10A to 10E LAUNDRY WASTEWATER TREATMENT DEVICE 11 LAUNDRY WASTEWATER 12 LAUNDRY WASTEWATER TANK 13 ACTIVATED SLUDGE 14 BIOLOGICAL TREATMENT TANK 15 TREATED LAUNDRY WASTEWATER 16 TREATED WATER TANK 18 DISCHARGED WATER 19-1 FIRST COD MEASUREMENT DEVICE 19-2 SECOND COD MEASUREMENT DEVICE 21 LAUNDRY WASTEWATER BIOLOGICAL TREATMENT PREPARATION 22 TREATMENT PREPARATION SUPPLYING DEVICE 31 EXTRACTION LINE 32 FREEZE-DRYING DEVICE