STRAIN OF PSEUDOMONAS AERUGINOSA 9# AND APPLICATIONS THEREOF

Abstract

A strain of Pseudomonas aeruginosa 9 # and its applications are disclosed, which relates to the technical field of microorganisms. The deposit number of the Pseudomonas aeruginosa 9 # of the disclosure is CCTCC NO: M 2021178. The strain of Pseudomonas aeruginosa 9 # can inhibit the growth of the Ustilaginodea vixens strain YY7850, which is the first instance that the Pseudomonas aeruginosa strain has been found to antagonize the pathogen of rice false smut. In view of the ability of strain 9 # to inhibit the pathogen of rice false smut, the strain has the potential to be developed into a biocontrol bacterium of rice false smut.

Claims

1. A strain of Pseudomonas aeruginosa 9 #, wherein a deposit number of the Pseudomonas aeruginosa 9 # is CCTCC NO: M 2021178.

2. An application of the strain of Pseudomonas aeruginosa 9 # of claim 1 in antagonizing Ustilaginodea vixens.

3. An application of the strain of Pseudomonas aeruginosa 9 # of claim 1 in preventing and treating rice false smut.

4. An application of the strain of Pseudomonas aeruginosa 9 # of claim 1 in a preparation of microbial agents for preventing and treating rice false smut.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0013] In order to explain the embodiments of the present disclosure or the technical solutions in the prior art more clearly, the following drawings that need to be used in the description of the embodiments or the prior art will be briefly introduced. Obviously, the drawings in the following description are only embodiments of the present disclosure. For those of ordinary skill in the art, other drawings can be obtained based on the drawings disclosed without creative work.

[0014] FIG. 1 shows the colony morphology of the strain 9 # of the disclosure.

[0015] FIG. 2 is the phylogenetic tree of the strain 9 # of the disclosure.

[0016] FIG. 3 shows the antibacterial effect of the strain 9 # of the disclosure on the Ustilaginodea virens strain YY7850.

[0017] Where, A is the control, and B is the antagonism diagram.

DETAILED DESCRIPTION OF THE EMBODIMENTS

[0018] Technical solutions of the present disclosure will be clearly and completely described below with reference to the embodiments. Obviously, the described embodiments are only part of the embodiments of the present disclosure, not all of them. Based on the embodiments of the disclosure, all other embodiments made by those skilled in the art without sparing any creative effort should fall within the protection scope of the disclosure.

Embodiment 1 Screening and Identification of Strain 9 #

[0019] The antagonistic experiment of Ustilaginodea virens (Cooke) Takahashi was carried out on several bacterial strains isolated and purified from the soil of the experimental field of China National Rice Research Institute. A bacterial strain, named strain 9 #, was successfully screened through the antagonistic experiment. The colony morphology is shown in FIG. 1. After cultured on ordinary agar, the bacterium formed large and flat colonies, which were ground glass like, had metallic luster and ginger flavor, could produce blue-green pigment, and diffused in the culture medium.

[0020] Strain 9 # was identified as a strain of Pseudomonas aeruginosa by 16s sequencing and comparative analysis. The phylogenetic tree is shown in FIG. 2.

Embodiment 2 Antagonistic Test

[0021] (1) Strain Culture

[0022] A. Culture of Ustilaginodea Virens Strain YY7850:

[0023] The Ustilaginodea virens strain YY7850 preserved in the laboratory was inoculated on potato sucrose agar medium (PSA) plate and cultured at a constant temperature of 28° C. After the colonies grow, they are inoculated into potato sucrose liquid medium (PS), cultured at 150 rpm at a constant temperature of 28° C. for 7 days, and then filtered with eight layers of gauze to obtain spore suspension for standby.

[0024] Potato sucrose agar medium (PSA): PSA agar medium produced by Beijing Solarbio Technology Co., Ltd. was used. The PSA agar medium was prepared by adding 47.0 g PSA agar medium to each liter of water. After subpackaging, PSA agar medium was sterilized at 121° C. for 20 minutes under high pressure and damp heat. Then it was taken out and shaken well. After cooling, it was stored at 4° C. for standby.

[0025] Potato sucrose liquid medium (PS): 200 g potatoes were taken and 1000 mL of water was added. The potato was boiled with water for 20˜30 minutes, and was filtered with eight layers of gauze. 20 g sucrose was added to the filtrate, and the volume was fixed to 1 L. After high-pressure moist heat sterilization at 121° C. for 20 minutes, it was taken out and cooled for standby.

[0026] B. Culture of the Antagonistic Strain 9 #

[0027] Strain 9 # was inoculated on LB slant and cultured at a constant temperature of 37° C. for 1 day. After the colonies grew, they were inoculated into LB liquid medium, cultured at 150 rpm at a constant temperature of 37° C. for 1 day for standby.

[0028] LB slant: LB agar medium produced by Sangon Biotech (Shanghai) Co., Ltd. was used as the culture medium. LB slant was prepared by adding 35.0 g LB agar medium per liter of water. After subpackaging, it was sterilized with 121° C. high-pressure damp heat for 20 minutes, and the slant was placed while it was hot, and was stored at 4° C. for standby after the slant was cooled.

[0029] LB liquid medium: LB liquid medium produced by Sangon Biotech (Shanghai) Co., Ltd. was used. LB liquid medium was prepared by adding 35.0 g LB liquid medium per liter of water. After subpackaging, it was sterilized with 121° C. high-pressure damp heat for 20 minutes, taken out and cooled for standby.

[0030] (2) Antagonistic Test

[0031] 150 μL spore suspension (the spore number was 1×10.sup.6 cfu/mL) of Ustilaginodea virens strain YY7850 cultured in step (1) was taken and evenly coated on a sterile PSA plate with a diameter of 9 cm with a sterilized coating rod. After 28° C. constant temperature pre culture for 1 day, a sterile filter paper with a diameter of 6 mm was taken and placed on the middle of the PSA plate. 5 μL of antagonistic strain 9 # bacterial solution cultured in step (1) was added (the plate coated with only Ustilaginodea virens strain YY7850 spore suspension without antagonistic 9 # was as the control plate). It was cultured at a constant temperature of 28° C. in an upright position until the control plate was full of colonies. The radius of the antibacterial circle was measured, and the antagonistic result is shown in FIG. 3. It can be seen from FIG. 3 that strain 9 # had obvious antibacterial effect on Ustilaginodea virens strain YY7850, and the radius of the antibacterial circle was 3.3 cm. Thus, the screened strain 9 # has the ability to inhibit the pathogen of rice false smut, and has the potential to develop into a biocontrol bacterium of rice false smut.

[0032] The above description of the disclosed embodiments enables the skilled in the art to achieve or use the disclosure. Multiple modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein may be achieved in other embodiments without departing from the spirit or scope of the disclosure. The present disclosure will therefore not be restricted to these embodiments shown herein, but rather to comply with the broadest scope consistent with the principles and novel features disclosed herein.