Microparticle separation apparatus assembly comprising multiple separable panels

Abstract

The present invention relates to a microparticle separation apparatus assembly comprising multiple separable panels, more specifically, to a microparticle separation apparatus assembly having a novel structure, comprising: a first panel comprising a magnetic microstructure for applying magnetophoretic force to microparticles; and a second panel, which can be separated from the first panel and through which a sample comprising microparticles passes, wherein the first panel comprising the magnetic microstructure can be recycled.

Claims

1. A microparticle separation apparatus assembly comprising: a first panel comprising a magnetic microstructure; a second panel detachable from the first panel and including a microchannel structure through which the sample passes; and a thin film portion between the second panel and the first panel, the thin film portion having a thickness of 50 um or less and made of PET, PI, PE, PP, PMMA or glass, wherein the magnetic microstructures included in the first panel include a pattern formed in a direction perpendicular to the direction in which the sample is introduced.

2. The microparticle separation device assembly according to claim 1, wherein the magnetic microstructures included in the first panel are inclined at an angle with respect to a flow direction of cells in the second panel.

3. The microparticle separation device assembly of claim 1, further comprising a coupling portion for coupling the first panel and the second panel.

4. The microparticle separation device assembly of claim 3, wherein the coupling portion comprises a vacuum applying portion, or a magnet.

5. The fine particle separator assembly of claim 3, wherein the coupling portion includes concave and convex portions formed in correspondence with the first panel and the second panel.

6. The apparatus of claim 1, wherein the first panel comprises n first 1-1 panels, a 1-2 panel, and a first-n panel are connected.

7. The apparatus of claim 1, wherein the second panel comprises n second-1 panels, second-2 panels, and a second-n panel are connected.

8. The fine particle separation apparatus according to claim 6, wherein the first-n panel is in contact with the second-n panel and the thin film part.

9. The microparticle separation device assembly of claim 8, wherein the magnetic microstructures included in the first-n panel are perpendicular to the sample flow direction.

10. The microparticle separation device assembly of claim 1, wherein the first panel is located on top of the second panel.

11. The microparticle separation device assembly of claim 1, wherein the first panel is located below the second panel.

12. The microparticle separation device assembly of claim 1, wherein the first panel and the second panel further comprise an alignment portion for aligning the sample fluid channel with the magnetic microstructure.

13. The fine particle separation apparatus according to claim 7, wherein the first-n panel is in contact with the second-n panel and the thin film part.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

(1) FIGS. 1 and 2 show a microparticle separation apparatus assembly according to the present invention;

(2) FIGS. 3 to 5 show magnetic microstructure patterns of first and second panels in a microparticle separation apparatus assembly according to an exemplary embodiment of the present invention;

(3) FIG. 6 shows various exemplary embodiment of a coupling portion in the microparticle separation apparatus according to the present invention;

(4) FIGS. 7 to 9 show the microparticle separation apparatus manufactured according to the exemplary embodiment of the present invention;

(5) FIG. 10 shows cells separated by using the microparticle separation apparatus manufactured according to the exemplary embodiment of the present invention;

(6) FIGS. 11 and 12 show a microparticle separation apparatus manufactured according to another exemplary embodiment of the present invention; and

(7) FIG. 13 shows cells separated by using the microparticle separation apparatus manufactured according to an exemplary embodiment of the present invention.

DETAILED DESCRIPTION OF THE ILLUSTRATED EMBODIMENTS

(8) Hereinafter, exemplary embodiments of the present invention will be described in further details. However, the present invention is not limited to the following embodiments.

Manufacturing Example 1

(9) FIG. 7 shows a first panel and a second panel that are made separable from each other. FIG. 8 shows the first and second panels that are made separable from each other that are connected to a vacuum pump and bonded together by applying vacuum. FIG. 9 shows the first and second panels that are bonded to the thin film portion by placing a magnet over the second panel.

(10) As shown in FIG. 7, the first panel (200) comprises a magnetic microstructure (300) and a vacuum application part (510) for applying vacuum near the magnetic microstructure. The second panel (100) comprises a microchannel structure (110) that is separated from the first panel and can be separated and captured as a sample passes through it.

(11) As shown in FIG. 9, the first and second panels made separable from each other are bonded to the thin film portion by putting them over a ferromagnet and placing a magnet (600) over the second panel.

Test Example 1

(12) FIG. 10 shows a fluoroscopic image of cells that are separated and captured on a capturing portion by using the microparticle separation apparatus assembly made separable according to the present invention that is manufactured in the manufacturing example 1.

Manufacturing Example 2

(13) As shown in the schematic diagram of FIG. 11, a microparticle separation apparatus assembly was manufactured by connecting a plurality of separated panels.

(14) A (1-1)th panel (210) comprising a magnetic microstructure (310) for separating a sample and a (1-2)th panel (220) separated from the (1-1)th panel and comprising a magnetic microstructure (320) for capturing a sample, which is formed in a direction perpendicular to the direction in which a sample enters, were manufactured.

(15) Moreover, a (2-1)th panel (110) connected to the (1-1)th panel (210) and comprising a channel for separating a sample and a (2-2)th panel (120) connected to the (1-2)th panel and comprising a chamber for capturing cells within a separated sample were manufactured.

(16) After the manufacture of a thin-film portion, a firstassembly (10) in which the (1-1)th panel and the (2-1)th panel adjoin with the thin film portion in between and a second assembly (10-1) formed by connecting the (1-2)th panel and the (2-2)th panel are laminated, and then the first assembly (10) and the second assembly (10-1) are connected by a connecting portion (810) so that a sample separated on the (2-1)th panel is captured on the (2-2)th panel. The connection state is depicted in FIG. 12.

(17) FIG. 13 shows a fluoroscopic image of cells that are separated and captured on a capturing portion by using the microparticle separation apparatus assembly that is manufactured in the above manufacturing example.