Bispecific recombinant protein and use thereof

Abstract

Provided is a bispecific recombinant protein, comprising a high affinity tumor-targeting arm and a low affinity fusion protein blocking the interaction of CD47 with SIRPα. The antibody corresponding to the high affinity tumor-targeting arm does not bind to CD47, and its binding affinity to the target antigen on the tumor cell is at least 6 times as great as the binding affinity of monomer fusion protein homodimer corresponding to the low affinity fusion protein blocking the interaction of CD47 with SIRPα, to a CD47 on the tumor cell, wherein the low affinity fusion protein blocking the interaction of CD47 with SIRPα comprises a SIRPα extracellular truncation. Also provided are nucleic acid molecules encoding recombinant proteins and the use of the recombinant proteins and nucleic acid molecules in the manufacture of a medicament for treating tumors.

Claims

1. A bispecific recombinant protein, wherein the bispecific recombinant protein comprises a high affinity tumor-targeting arm and a fusion protein with low affinity for blocking the interaction between CD47 and SIRPα; wherein the bispecific recombinant protein has a configuration comprising a left arm and a right arm which are symmetrically arranged, wherein the high affinity tumor-targeting arm is arranged in the left arm, the fusion protein with low affinity for blocking the interaction between CD47 and SIRPα is arranged in the right arm; wherein the high affinity tumor-targeting arm and the fusion protein with low affinity for blocking the interaction between CD47 and SIRPα are combined via intermolecular force, interchain disulfide bond and salt bond; wherein when the high affinity tumor-targeting arm targets CD20, the high affinity tumor-targeting arm comprises SEQ ID No: 16 and SEQ ID No: 17, the fusion protein with low affinity for blocking the interaction between CD47 and SIRPα comprises SEQ ID No: 26; when the high affinity tumor-targeting arm targets EGFR, the high affinity tumor-targeting arm comprises SEQ ID No: 19 and SEQ ID No: 8, the fusion protein with low affinity for blocking the interaction between CD47 and SIRPα comprises SEQ ID No: 26; when the high affinity tumor-targeting arm targets Her2, the high affinity tumor-targeting arm comprises SEQ ID No: 20 and SEQ ID No: 21, or SEQ ID No: 22 and SEQ ID No: 23, the fusion protein with low affinity for blocking the interaction between CD47 and SIRPα comprises SEQ ID No: 26 or SEQ ID No: 27; or when the high affinity tumor-targeting arm targets PD-L1, the high affinity tumor-targeting arm comprises SEQ ID No: 24 and SEQ ID No: 13, the fusion protein with low affinity for blocking the interaction between CD47 and SIRPα comprises SEQ ID No: 26.

2. A nucleic acid molecule encoding the bispecific recombinant protein of claim 1, the nucleic acid molecule encoding the high affinity tumor-targeting arm and the nucleic acid molecule encoding the fusion protein with low affinity for blocking the interaction between CD47 and SIRPα are presented together in the same DNA strand, or the nucleic acid molecule encoding the high affinity tumor-targeting arm and the nucleic acid molecule encoding the fusion protein with low affinity for blocking the interaction between CD47 and SIRPα are presented in separate DNA strands.

3. An expression vector comprising the nucleic acid molecule of claim 2.

4. A host cell comprising the expression vector of claim 3.

5. A method for preparing a bispecific recombinant protein, wherein the method employs the cell of claim 4 to express the recombinant protein.

6. A pharmaceutical composition comprising the bispecific recombinant protein of claim 1, and an optional adjuvant, excipient or pharmaceutically acceptable carrier.

7. The bispecific recombinant protein of claim 1, wherein the high affinity tumor-targeting arm and the fusion protein with low affinity for blocking the interaction between CD47 and SIRPα are heterodimerized by knobs-into-holes.

8. A bispecific recombinant protein, wherein the bispecific recombinant protein comprises a high affinity tumor-targeting arm and a fusion protein with low affinity for blocking the interaction between CD47 and SIRPα; wherein the bispecific recombinant protein has a configuration comprising a left arm and a right arm which are symmetrically arranged, wherein the high affinity tumor-targeting arm is arranged in the left arm, the fusion protein with low affinity for blocking the interaction between CD47 and SIRPα is arranged in the right arm; wherein the length of the right arm is configured for the distance from the epitope to which the left arm binds to the membrane surface of the target cell; wherein the high affinity tumor-targeting arm is a half antibody specific to CD20; wherein the high affinity tumor-targeting arm and the fusion protein with low affinity for blocking the interaction between CD47 and SIRPα are combined via intermolecular force, covalent bond and salt bond; and wherein the high affinity tumor-targeting arm targets CD20, the high affinity tumor-targeting arm comprises SEQ ID No: 18 and SEQ ID No: 4, the fusion protein with low affinity for blocking the interaction between CD47 and SIRPα comprises SEQ ID No: 26.

9. A nucleic acid molecule encoding the bispecific recombinant protein of claim 8, the nucleic acid molecule encoding the high affinity tumor-targeting arm and the nucleic acid molecule encoding the fusion protein with low affinity for blocking the interaction between CD47 and SIRPα are presented together in the same DNA strand, or the nucleic acid molecule encoding the high affinity tumor-targeting arm and the nucleic acid molecule encoding the fusion protein with low affinity for blocking the interaction between CD47 and SIRPα are presented in separate DNA strands.

10. An expression vector comprising the nucleic acid molecule of claim 9.

11. A host cell comprising the expression vector of claim 10.

12. A method for preparing a bispecific recombinant protein, wherein the method employs the cell of claim 11 to express the recombinant protein.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

(1) FIG. 1 shows the structure of the insert of the present disclosure to be inserted into the expression vector.

(2) FIG. 2 shows the plasmid map of an exemplary expression vector pCHO-TE2 of the present disclosure.

(3) FIG. 3 shows the structure of an exemplary embodiment of the recombinant proteins of the present disclosure.

(4) FIG. 4A-4B show the SDS-PAGE electrophoretogram of the recombinant proteins purified by protein A. Lanes 1-6 of FIG. 4A show reduced samples, wherein, 1: Marker; 2: Anti-CD20 mAb (Ofatumumab); 3: Anti-CD47 mAb (Hu5F9-G4); 4: SIRPα D1-Fc; 5: Ofa-Fc1-D1-Fc2; 6: Ofa-Fc1-D1-D2-D3-Fc2. Lanes 7-12 of FIG. 4A show non-reduced samples, wherein, 7: Marker; 8: Anti-CD20 mAb (Ofatumumab); 9: Anti-CD47 mAb (Hu5F9-G4); 10: SIRPα D1-Fc; 11: Ofa-Fc1-D1-Fc2; 12: Ofa-Fc1-D1-D2-D3-Fc2. Lane 1 of FIG. 4B: the non-reduced sample of Ofa-Fc1-D1-D2-Fc2; Lane 2 of FIG. 4B; Marker; Lane 3 of FIG. 4B: the reduced sample of Ofa-Fc1-D1-D2-Fc2.

(5) FIG. 5 shows the ELISA detection results of the binding affinity of the recombinant proteins of the present disclosure to human CD47 (protein level).

(6) FIG. 6 shows the flow cytometry results of the binding affinity of the recombinant proteins of the present disclosure to human CD47 (cellular level):

(7) FIG. 7 shows the flow cytometry results of the binding affinity of the recombinant proteins of the present disclosure to the target corresponding to the left arm. Hu5F9-G4, Ofa-Fc1-D1-Fc2 and SIRPα D1-Fc refer to the average fluorescence intensity after the binding of the samples Hu5F9-G4, Ofa-Fc1-D1-Fc2 and SIRPα D1-Fc to the Raji cells which are not blocked by anti-CD47 antibody Hu5F9-G4(Fab)2, Hu5F9-G4 (b), Ofa-Fc1-D1-Fc2(b) and SIRPα D1-Fc(b) refer to the average fluorescence intensity after the binding of Hu5F9-G4, Ofa-Fc1-D1-Fc2 and SIRPα D1-Fc to Raji cells which are blocked with anti-CD47 antibody Hu5F9-G4(Fab)2.

(8) FIG. 8 shows the flow cytometry results of the binding affinity of the recombinant proteins of the present disclosure to the cells expressing both the CD47 and the target corresponding to the left arm. FIG. 8A corresponds to Raji cells (CD20+CD47); FIG. 8B corresponds to SKBR-3 cells (Her2+CD47); FIG. 8C corresponds to A431 cells (EGFR+CD47); FIG. 8D corresponds to NCI-H441 cells (PD-L1+CD47).

(9) FIG. 9 shows the ELISA results of the competitive binding of the recombinant proteins of the present disclosure to SICRα D1-Fc and anti-CD47 antibodies against CD47.

(10) FIG. 10 shows the flow cytometry results of the binding affinity of the recombinant proteins of the present disclosure and its high affinity variants to CD47, anti-CD47 antibodies and anti-CD20 antibodies to human CD47.

(11) FIG. 11 shows the results of the immunological safety evaluation assay of the recombinant proteins of the present disclosure in vitro.

(12) FIG. 12 shows the results of the pharmacodynamic assay of the recombinant proteins of the present disclosure on a NSG mouse model of subcutaneously transplanted Raji lymphoma.

(13) FIG. 13 shows the detection results of B cell content at 96 hours after treating the Hu-NSG mice with different samples at a same dose. Wherein, FIG. 13A corresponds to 0.9% physiological saline; FIG. 13B corresponds to Hu5F9-G4 (6.7 μg/mouse); FIG. 13C corresponds to Ofa-Fc1-D1-Fc2 (5 μg/mouse).

(14) FIG. 14 shows the results of the early immunological safety evaluation assay of Ofa-Fc1-D1-Fc2 and Ofa-Fc1-D1m-Fc2 in Hu-NSG mice (FACS analysis of immune cell types) in vivo. FIG. 14A-D correspond to Ofa-Fc1-D1-Fc2 (1 μg/mouse), wherein FIGS. 14A and 14B show the detection results before administration, and FIGS. 14C and 14D show the detection results at 72 hours after administration. FIG. 14E-H correspond to Ofa-Fc1-DP1.sup.m-Fc2 (1 μg/mouse), wherein FIG. 14E and FIG. 14F show the detection results before administration, FIG. 14G and FIG. 14H show the detection results at 72 hours after administration.

(15) FIG. 15 shows the immunophenotyping FACS analysis at 96 hours after treating the Hu-NSG mice with different samples at a high dose. FIG. 15A-B show the results at 96 hours after administration, wherein Fig. A corresponds to Hu5F9-G4 (200 μg/mouse) and FIG. 15B corresponds to Ofa-Fc1-D1-Fc2 at a high dose (150 μg/mouse). FIG. 15C-D show the results at 14 days after administration, wherein FIG. 15C corresponds to Hu5F9-G4 (200 μg/mouse) and FIG. 15D corresponds to Ofa-Fc1-D1-Fc2 at a high dose (150 μg/mouse).

(16) FIG. 16 shows the results of the binding of the recombinant proteins of the present disclosure to the dual targets (Her2, CD47) in SKBR-3. FIG. 16A shows the detection results of Anti-Her2(T)-Fc1-D1-Fc2 and Anti-Her2(T)-Fc1-D1-D2-Fc2; and FIG. 16B shows the detection results of Anti-Her2(P)-Fc1-D1-Fc2 and Anti-Her2(P)-Fc1-D1-D2-Fc2.

(17) FIG. 17A-E show the amino acid sequences and DNA sequences corresponding to the exemplary proteins of the present disclosure.

(18) FIG. 18A shows the effect of Ofa-Fc1-D1-Fc2 on the number of RBCs of cynomolgus monkeys at two doses; FIG. 18B shows the effect of Ofa-Fc1-D1-Fc2 on the hemoglobin of cynomolgus monkeys at two doses.

(19) FIG. 19 shows the detection results of Ofa-Fc1-D1-Fc2 on B cell content of cynomolgus monkeys at two doses.

(20) FIG. 20 shows the effect of Ofa-Fc1-D1-Fc2 and Rituxan® on the growth of human B-cell lymphoma Daudi subcutaneous xenografts.

(21) FIG. 21 shows the effect of Ofa-Fc1-D1-Fc2 and Rituxan® on the body weight of tumor-bearing mice.

(22) FIG. 22 shows the effect of Ofa-Fc1-D1-Fc2 and Rituxan® on human B-cell lymphoma Daudi subcutaneous xenografts.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENT

(23) In order to promote an understanding of the present disclosure, the present disclosure will be illustrated with reference to certain examples and some specific terms described below. However, it should be understood that these specific examples are not intended to limit the scope of the present disclosure, any alterations and further modifications to the described examples, as well as any further applications of the disclosure, will all be obvious to those skilled in the art.

(24) Recombinant Protein

(25) As used herein, the term “recombinant protein” refers to a protein that is artificially engineered/constructed, rather than a naturally occurring protein. “Recombinant” contained in the term “recombinant protein” of the present disclosure does not denote a mode of production, and is merely used to indicate that “recombinant protein” does not naturally occur. The recombinant protein of the present disclosure may be an expressed protein and may be an assembled protein.

(26) Optionally, the bispecific recombinant protein of the present disclosure comprises a ‘high affinity tumor-targeting arm’ and a ‘fusion protein with low affinity for blocking the interaction between CD47 and SIRPα’.

(27) As used herein, “high affinity tumor-targeting” refers to that the binding affinity of the recombinant protein of the present disclosure to a tumor is higher than or substantially equivalent to the binding affinity of tumor-binding antibody drugs in the prior art to the tumor, wherein the binding affinity of tumor-binding antibody drugs in the prior art to the tumor generally has anEC50 at nM or pM level. Preferably, the ratio of the binding affinity of the antibody corresponding to the ‘high affinity tumor-targeting arm’ to the target antigen on a tumor cell to the binding affinity of the homodimer of the monomer fusion protein corresponding to the ‘fusion protein with low affinity for blocking the interaction between CD47 and SIRPα’ to CD47 on a tumor cell is at least 6, optionally 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 or more, or any value between them. Optionally, in the recombinant protein of the present disclosure, the ratio of the binding affinity of the antibody corresponding to the ‘high affinity tumor-targeting arm’ to the target antigen on a tumor cell to the binding affinity of the homodimer of SIRPa-Fc corresponding to the ‘fusion protein with low affinity for blocking the interaction between CD47 and SIRPα’ to CD47 on a tumor cell is at least 6, optionally 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 or more, or any value between them.

(28) As used herein, “low affinity for blocking the interaction between CD47 and SIRPα” refers to that the recombinant protein of the present disclosure is capable of blocking the interaction between CD47 and SIRPα with a low affinity. Preferably, the binding affinity of the ‘fusion protein with low affinity for blocking the interaction between CD47 and SIRPα’ to CD47 is not higher than the binding affinity of ‘the homodimer of a monomer fusion protein comprising an extracellular truncated valiant of SIRPα’ to CD47. More preferably, the binding affinity of which is not higher than the binding affinity of humanized SIRPα-Fc fusion protein to CD47.

(29) The bispecific recombinant protein described herein can significantly enhance the tumor-targeting saturation binding abundance of a recombinant protein with an effect of modulating the function of macrophages and reduce non-tumor target side effects, as long as the ratio of the binding affinity of the antibody corresponding to the ‘high affinity tumor-targeting arm’ to the target antigen on tumor cell to the binding affinity of the homodimer of the monomer fusion protein corresponding to the ‘fusion protein with low affinity for blocking the interaction between CD47 and SIRPα to CD47’ on tumor cell is at least 6.

(30) The method for detecting the binding affinity used herein is well known to those skilled in the art, including but is not limited to, ELISA and/or flow cytometry.

(31) Optionally, the fusion protein of a extracellular truncated variant of signal regulatory protein alpha α (including a wild-type truncated variant and its non-high affinity variant) and a Fc described in the present disclosure and a half antibody can form a heterodimer by modifying the heavy chain of antibody, specifically, can be heterodimerized by Knobs-into-holes and/or mediated by interchain disulfide bond and/or salt bond to forma recombinant protein.

(32) Optionally, when “tumor-targeting arm” or “half antibody” or “left arm” or “half antibody structure” or “Ig molecular monomer” and “CD47-targeting arm” or “right arm” or “fusion protein of a truncated variant of signal regulatory protein a and Fc” or “SIRPα-Fc” are heterodimerized by knobs-into-holes to forma recombinant protein, the structure of the obtained recombinant protein is as shown in FIG. 3. Wherein, the left arm is a tumor-targeting half antibody, including but is not limited to anti-5T4 half antibody, anti-AGS-16 half antibody, anti-ALK1 half antibody, anti-ANG-2 half antibody, anti-B7-H3 half antibody, anti-B7-H4 half antibody, anti-c-fms half antibody, anti-c-Met half antibody, anti-CA6 half antibody, anti-CD123 half antibody, anti-CD19 half antibody, anti-CD20 half antibody, anti-CD22 half antibody, anti-EpCAM half antibody, anti-CD30 half antibody, anti-CD32b half antibody, anti-CD37 half antibody, anti-CD38 half antibody, anti-CD40 half antibody, anti-CD52 half antibody, anti-CD70 half antibody, anti-CD74 half antibody, anti-CD79b half antibody, anti-CD98 half antibody, anti-CEA half antibody, anti-CEACAM5 half antibody, anti-CEDN18.2 half antibody, anti-CLDN6 half antibody, anti-CS1 half antibody, anti-CXCR4 half antibody, anti-DLL-4 half antibody, anti-EGFR half antibody, anti-EGP-1 half antibody, anti-ENPP3 half antibody, anti-EphA3 half antibody, anti-ETBR half antibody, anti-FGER2 half antibody, anti-EN half antibody, anti-FR-α half antibody, anti-GCC half antibody, anti-GD2 half antibody, anti-GPC-3 half antibody, anti-GPNMB half antibody, anti-HER2 half antibody, anti-HER3 half antibody, anti-HLA-DR half antibody, anti-ICAM-1 half antibody, anti-IGF-1R half antibody, anti-IL-3R half antibody, anti-LIV-1 half antibody, anti-MSLN half antibody, anti-MUC16 half antibody, anti-MUC1 half antibody, anti-NaPi2b half antibody, anti-nectin-4 half antibody, anti-Notch 2 half antibody, anti-Notch 1 half antibody, anti-PD-L1 half antibody, anti-PD-L2 half antibody, anti-PDGFR-α half antibody, anti-PS half antibody, anti-PSMA half antibody, anti-SLTRK6 half antibody, anti-STEAP1 half antibody, anti-TEM1 half antibody, anti-VEGFR half antibody, anti-CD25 half antibody, anti-CD27L half antibody, anti-DKK-1 half antibody, anti-CSF-1R half antibody, anti-MSB0010718C half antibody, anti-BCMA half antibody, anti-CD138 half antibody. The right arm is a fusion protein formed by linking an extracellular truncated variant of SIRPα (including human wild-type SIRPα and its non-high affinity mutant) and hinge region and Fc region of IgG antibody. Wherein the Fc region of the right arm is a hole mutant, the corresponding Fc region of the left arm is a knob mutant; or the right arm is a knob mutant, the corresponding Fc region of the left arm is a hole mutant. As known to those skilled in the art, the Fc region can also contain multiple mutations of holes and/or knobs simultaneously.

(33) Table 1 shows the configuration of exemplary recombinant protein molecules.

(34) TABLE-US-00001 TABLE 1 Exemplary recombinant protein molecules No. Left arm Right arm 1 Anti-CD20-Fc D1-Fc2 2 Anti-CD20-Fc1 D1-D2-Fc2 3 Anti-CD20-Fc1 D1-D2-D3-Fc2 4 Anti-CD20-Fc1 D1.sup.m-Fc2 5 Anti-CD20-Fc1 D1.sup.m-D2-Fc2 6 Anti-PD-L1-Fc1 D1-Fc2 7 Anti-PD-Ll-Fc1 D1-D2-Fc2 8 Anti-PD-L1-Fc1 D1-D2-D3-Fc2 9 Anti-PD-L1-Fc1 D1.sup.m-Fc2 10 Anti-PD-L1-Fc1 D1.sup.m-D2-Fc2 11 Anti-EGFR-Fc1 D1-Fc2 12 Anti-EGFR-Fc1 D1-D2-Fc2 13 Anti-EGFR-Fc1 D1-D2-D3-Fc2 14 Anti-EGFR-Fc1 D1.sup.m-Fc2 15 Anti-EGFR-Fc1 D1.sup.m-D2-Fc2 16 Anti-Her2-Fc1 D1-Fc2 17 Anti-Her2-Fc1 D1-D2-Fc2 18 Anti-Her2-Fc1 D1-D2-D3-Fc2 19 Anti-Her2-Fc1 D1.sup.m-Fc2 20 Anti-Her2-Fc1 D1.sup.m-D2-Fc2 Notes: D1.sup.m represents a high affinity mutant of SIRPα extracellular truncated variant D1; D1represents the extracellular D1 domain of human wild-type SIRPα or its non-high affinity mutant; Fc represents a wild-type Fc region; Fc1 represents an Fc region having a hole or holes mutation, Fc2 represents an Fc region having a knob or knobs mutation.

(35) The corresponding amino acid sequence and DNA sequence of the recombinant proteins of the present disclosure are shown in FIG. 17A-E and the sequence listing file of the present disclosure.

(36) Antibody

(37) As used herein, the term “antibody” or “immunoglobulin” is a heterotetrameric glycoprotein of about 150,000 Daltons having a same structural feature, composing of two identical light chains (L) and two identical heavy chains (H). Each light chain is linked to a heavy chain by covalent disulfide bond(s), while the number of interchain disulfide bonds of the heavy chains varies between different immunoglobulin isotypes. Each heavy and light chain also has regularly spaced intrachain disulfide bonds. Each heavy chain has, at one end, a variable domain (V.sub.H) followed by a number of constant domains. Each light chain has a variable domain at one end (V.sub.L) and a constant domain at its other end; the constant domain of the light chain is aligned with the first constant domain of the heavy chain, and the light chain variable domain is aligned with the variable domain of the heavy chain. Particular amino acid residues are believed to form an interface between the light- and heavy-chain variable domains.

(38) Tumor-Targeting Arm

(39) As used herein, the term “tumor-targeting arm” or “half antibody” or “left arm” or “half antibody structure” or “Ig molecule monomer” refers to a heterodimeric glycoprotein composed of a light chain (L) and a heavy chain (H) of the antibody, which is the basic structure of the immunoglobulin molecule, and these terms can be used interchangeably herein. Its molecular weight is half of the molecular weight of the corresponding antibody, about 75,000 Daltons, wherein the light chain is linked to the heavy chain by a covalent disulfide bond. The heavy and light chain also has regularly spaced intrachain disulfide bonds. Each heavy chain has, at one end, a variable domain (V.sub.H) followed by a number of constant domains. Each light chain has a variable domain at one end (V.sub.L) and a constant domain at its other end; the constant domain of the light chain is aligned with the first constant domain of the heavy chain, and the light chain variable domain is aligned with the variable domain of the heavy chain. Particular amino acid residues are believed to form an interface between the light- and heavy-chain variable domains.

(40) As used herein, the term “tumor-targeting arm”, “half antibody” or “left arm” or “half antibody structure” or “Ig molecular monomer” may be an IgG protein targeting various tumors. The target molecules include, but are not limited to 5T4, AGS-16, ALK1, ANG-2, B7-H3, B7-H4, c-fms, c-Met, CA6, CD123, CD19, CD20, CD22, EpCAM, CD30, CD32b, CD37, CD38, CD40, CD52, CD70, CD74, CD79b, CD98, CEA, CEACAM5, CLDN18.2, CLDN6, CS1, CXCR4, DLL-4, EGFR, EGP-1, ENPP3, EphA3, ETBR, FGFR2, FN, FR-α, GCC, GD2, GPC-3, GPNMB, HER2, HER3, HLA-DR, ICAM-1, IGF-1R, IL-3R, LW-1, MSLN, MUC16, MUC1, NaPi2b, nectin-4, Notch 2, Notch 1, PD-L1, PD-L2, PDGFR-α, PS, PSMA, SLTRK6, STEAP1, TEM1, VEGFR, CD25, CD27L, DKK-1, CSF-1R, MSB0010718C, BCMA and CD138.

(41) The Fc sequence of “tumor-targeting arm”, “half antibody” or “left arm” or “half antibody structure” or “Ig molecular monomer” can employ a hole or holes mutant and/or a knob or knobs mutant.

(42) CD47-Targeting Arm

(43) As used herein, the term “CD47-targeting arm” or “right arm” or “fusion protein of a truncated variant of signal regulatory protein α and Fc” or “SIRPα-Fc” or ‘fusion protein for blocking the interaction between CD47 and SIRPα’ can be used interchangeably herein. As known to those skilled in the art, the “CD47-targeting arm” or “right arm” or “fusion protein of a truncated variant of signal regulatory protein a and Fc” or “SIRPα-Fc” or “fusion protein blocking the interaction between CD47 and SIRPα” has a variable molecular length. Optionally, the “CD47-targeting arm” or “right arm” or “fusion protein of a truncation of signal regulatory protein α and Fc” or “SIRPα-Fc” or “fusion protein blocking the interaction between CD47 and SIRPα” with a plurality of different molecular lengths can be formed by linking an extracellular truncated variant of SIRPα (including a human wild type SIRPα and its non-high affinity mutant) to the hinge region and Fc region of the IgG1 antibody. The IgG1 can be human IgG1.

(44) The Fc of the “CD47-targeting arm” or “right arm” or “fusion protein of a truncation of signal regulatory protein α and Fc” or “SIRPα-Fc” or “fusion protein for blocking the interaction between CD47 and SIRPα” can employ a hole or holes mutant and/or a knob or knobs mutant.

(45) As known to those skilled in the art, “tumor-targeting arm” or “half antibody” or “left arm” or “half antibody structure” or “Ig molecular monomer” and “CD47-targeting arm” or “right arm” or “signal regulatory protein atruncated variant-Fc fusion protein” or “SIRPα-Fc” or “fusion protein for blocking the interaction between CD47 and SIRPα” can form a heterodimeric recombinant protein by modifying the Fc fragment (region). Specifically, the recombinant proteins of the present disclosure can be obtained via two or three of the following bindings: intermolecular force, covalent bond (such as interchain disulfide bond) and salt bond. Optionally, the recombinant proteins of the present disclosure are obtained by the knobs-into-holes technique.

(46) Knobs-into-Holes Technique

(47) As used herein, the term “knobs-into-holes technology” or “knobs-into-holes” is using genetic engineering techniques to induce different mutations in two CH3 domains of the heavy chain, thereby promoting the heteroditnerization of the heavy chain. In this technology, a knob is made on one heavy chain and a hole is made on the other heavy chain, then the two heavy chains preferentially couple together to form an asymmetric antibody (Ridgway J B, et al. ‘Knobs-into-holes’ engineering of antibody CH3 domains for heavy chain heterodimerization. Protein Engineering, 1996, 9(7): 617-621). As known to those skilled in the art, a plurality of knobs and/or holes can be made on one heavy chain, and correspondingly, a plurality of holes and/or holes can also be made on the other heavy chain.

(48) SIRPα

(49) As used herein, the term “SIRPα” is signal regulatory protein α, also known as CD172a. Signal regulatory protein (SIRP) is a transmembrane glycoprotein including three family members, SIRPα (CD172a), SIRPβ (CD172b) and SIRPγ (CD172g). These three members have similar extramembranous region and different intramembranous region. The extramembranous region contains three immunoglobulin (Ig)-like regions, of which the first region belongs to Isk/region and the second and third regions belong to IgC region. The intramembranous region of SIRPα (CD172a) contains two inhibitory signal domains that transmit an inhibitory signal and inhibit the corresponding function of the cell. The intramembranous region of SIRPβ (CD172b) and SIRPγ (CD172g) is short and has no signal transduction region, but SIRPβ (CD172b) can transmit an activation signal via an adaptor protein (such asDAP12). SIRP proteins are mainly expressed in macrophages (Mφ), dendritic cells (DC) and neuronal cells. It specifically refers to human wild type SIRPα and its mutant with non-high affinity to CD47.

(50) Extracellular Truncated Variant of SIRPα

(51) “Extracellular truncated variant” is used in relation to a protein that has a transmembrane function. As used herein, “extracellular truncated variant of SIRPα” refers to a part or full of the amino acid sequence of the extrarnembranous region of human wild type SIRPα and its mutant with non-high affinity to CD47 that was selectively truncated.

(52) As used herein, the terms “D1”, “D2” and “D3” refer to the three Ig-like extracellular domains of SIRPα, which are successively D1 domain (Ig variable region-like domain, IgV region), D2 domain (Ig constant region-like domain, IgC region) and D3 domain (Ig constant region-like domain, IgC region) starting from N-terminus of the protein (Lee W Y, et al. The Role of cis Dimerization of Signal Regulatory Protein α (SIRPα) in Binding to CD47. J Biol Chem, 2010, 285 (49): 37953-37963).

(53) SIRPα-Fc Fusion Protein

(54) As used herein, the term “SIRPα-Fc fusion protein” refers to a fusion protein comprising an extracellular truncated variant of SIRPα, an adaptor sequence and an Fc region. The adaptor sequence and/or Fc region contained in the above sequences can be arbitrarily replaced according to the methods well known to those skilled in the art or with common adaptor sequences and/or Fc regions.

(55) In order to avoid the influence of glycosylation, the present disclosure mutates asparagine to alanine on D1 (Reference: Lee W Y, et al, Novel Structural Determinants on SIRPα, that Mediate Binding to CD47. Journal of Immunology, 2007, 179(11): 7741-7750).

(56) D1, D2 and D3 of the present disclosure also include corresponding adaptor sequence.

(57) Adaptor Sequence

(58) As used herein, the term “adaptor sequence” refers to an amino acid sequence linking an extracellular truncated variant of SIRPα and a binding sequence, optionally, the adaptor sequence is a hinge region of IgG antibody, optionally comprising a hinge region and a heavy chain CH1 domain of IgG. The adaptor sequence or hinge region sequence contained in the above sequences may be arbitrarily replaced according to the methods well known to those skilled in the art or with common adaptor sequences or hinge region sequences.

(59) Binding Sequence

(60) As used herein, the term “binding sequence” refers to a sequence that binds a ‘high affinity tumor-targeting arm’ to a ‘fusion protein with low affinity for blocking the interaction between CD47 and SIRPα’, optionally, the binding sequence comprises a hinge region and an Fc region; more optionally, the Fc region comprises a knob or knobs mutation(s) and/or a hole or holes mutation(s). The binding sequence, hinge region sequence or Fc region sequence contained in the above sequences may be arbitrarily replaced according to the methods well known to those skilled in the art or with common binding sequences, hinge region sequences or Fc region sequences.

(61) CD47

(62) CD47 is a transmembrane glycoprotein belonging to the immunoglobulin superfamily and is expressed on the cell surface of almost all cells including RBCs. Ligands for CD47 include integrin, throinbospondin-1 and signal regulatory protein (SIRP). CD47 has a variety of biological functions, including cell migration, T cell activation, dendritic cell activation, axonal development, and the like. In addition, CD47 can inhibit phagocytosis of macrophages by interacting with SIRPα. In this way, CD47 transmits a so-called “don't eat me” signal that protects normal cells such as RBCs, B cells, and T cells from being phagocytosed by macrophages.

(63) Ofa

(64) As used herein, the terms “Ofa”, “Ofatumumab” and “Anti-CD20 (Ofatumumab)” are used interchangeably herein and refer to the anti-CD20 antibody Ofatumumab.

(65) Obi

(66) As used herein, the terms “Obi”, “Obinutuzumab” and “Anti-CD20 (Obinutuzumab)” are used interchangeably herein and refer to the anti-CD20 antibody Obinutuzumab.

(67) Hu5F9-G4

(68) As used herein, the terms “Anti-CD47 mAb”, “anti-CD47 antibody” and “Hu5F9-G4” are used interchangeably herein and refer to the anti-CD47 antibody Hu5F9-G4.

(69) Anti-EGFR mAb

(70) As used herein, the terms “Anti-EGFR mAb” and “JMT101” are used interchangeably herein and refer to the anti-EGFR antibody JMT101. JMT101 is a humanized anti-EGFR monoclonal antibody, see BA03 of the patent ZL201210406288.3.

(71) Trastuzumab

(72) As used herein, the terms “trastuzumab”, “Trastuzumab”, “Anti-Her2(T) mAb” and “Herceptin” are used interchangeably herein and refer to the anti-Her2 antibody Trastuzumab.

(73) Pertuzumab

(74) As used herein, the terms “patezumab”, “Pertuzumab”, “Anti-Her2(P) mAb” and “Perjeta” are used interchangeably herein and refer to the anti-Her2 antibody Pertuzumab.

(75) Atezolizumab

(76) As used herein, the terms “Tecentriq” and “Atezolizumab” are used interchangeably herein and refer to the anti-PD-L1 antibody Atezolizumab.

(77) SIRPα D1-Fc

(78) As used herein, the terms “SIRPα D1-Fc” and “D1-Fc” are used interchangeably herein and refer to a dimer of the single-chain fusion protein SIRPα, D1-Fc.

(79) Ofa-Fc1

(80) Ofa-Fc1 refers to Ofatumumab half antibody having an Fc region with a hole mutation.

(81) Anti-Her2(T)-Fc1

(82) Anti-Her2(T)-Fc1 refers to Transtuzumab half antibody having an Fc region with a hole mutation.

(83) Anti-Her2(P)-Fc1

(84) Anti-Her2(P)-Fc1 refers to Pertuzumab half antibody having an Fc region with a hole mutation.

(85) Anti-EGFR-Fc1

(86) Anti-EGFR-Fc1 refers to anti-EGFR half antibody having an Fc region with a hole mutation.

(87) D1-Fc2

(88) D1-Fc2 refers to a fusion protein comprising D1 domain truncated from the extracellular domain of SIRPα and an Fc region having a knob mutation.

(89) D1-D2-Fc2

(90) D1-D2-Fc2 refers to a fusion protein comprising D1 and D2 domains truncated from the extracellular domain of SIRPα and an Fc region with a knob mutation.

(91) D1-D2-D3-Fc2

(92) D1-D2-D3-Fc2 refers to a fusion protein comprising D1, D2 and D3 domains truncated from the extracellular domain of SIRPα and an Fc region with a knob mutation.

(93) Treatment

(94) As used herein, the terms “treating” “therapy” and “treatment” are used interchangeably. The term “treating” includes controlling the progression of a disease, a disorder and a condition and related symptoms, preferably reducing or alleviating the influence of one or more symptoms of a disease, a disorder and a condition. This term includes cure of the disease or complete elimination of the symptom. This term includes remission of the symptom. This term also includes, but is not limited to, non-cure palliative treatment. The term “treating” includes administering to a subject a therapeutically effective amount of a pharmaceutical composition comprising the recombinant protein or the fusion protein of the present disclosure to prevent or delay, alleviate or relieve the progression of a disease, a disorder, a condition, or the influence of one or more symptoms of a disease, a disorder and a condition.

(95) Administration

(96) As used herein, the term “administration” refers to the delivery of a therapeutically effective amount of a pharmaceutical composition comprising the recombinant protein or the fusion protein of the present disclosure to a subject. The administration can be systemic or topical. The administration can be performed with a delivery device, such as a syringe. Mode of administration include, but is not limited to, embedding, snorting, spraying, injecting, and the like. Route of administration include inhalation, intranasal, oral, intravenous, subcutaneous or intramuscular administration and the like.

(97) TABLE-US-00002 TABLE 2-1 Correspondence between sequence name and sequence number Sequence number Sequence name SEQ ID NO: 1 Ofa heavy chain amino acid sequence SEQ ID NO: 2 Ofa light chain amino acid sequence SEQ ID NO: 3 Obi heavy chain amino acid sequence SEQ ID NO: 4 Obi/Obi-Fc1 light chain amino acid sequence SEQ ID NO: 5 Hu5F9-G4 heavy chain amino acid sequence SEQ ID NO: 6 Hu5F9-G4 light chain amino acid sequence SEQ ID NO: 7 JMT101 heavy chain amino acid sequence SEQ ID NO: 8 JMT101/Anti-EGFR-Fc1 light chain amino acid sequence SEQ ID NO: 9 Trastuzumab heavy chain amino acid sequence SEQ ID NO: 10 Trastuzumab light chain amino acid sequence SEQ ID NO: 11 SIRPα D1-Fc amino acid sequence SEQ ID NO: 12 Atezolizumab heavy chain amino acid sequence SEQ ID NO: 13 Atezolizumab/Anti-PD-L1(Ate)-Fc1 light chain amino acid sequence SEQ ID NO: 14 D1-Fc2 DNA sequence SEQ ID NO: 15 D1-D2-Fc2 DNA sequence SEQ ID NO: 16 Ofa-Fc1 heavy chain amino acid sequence SEQ ID NO: 17 Ofa-Fc1 light chain amino acid sequence SEQ ID NO: 18 Obi-Fc1 heavy chain amino acid sequence SEQ ID NO: 19 Anti-EGFR-Fc1 heavy chain amino acid sequence SEQ ID NO: 20 Anti-Her2(T)-Fc1 heavy chain amino acid sequence SEQ ID NO: 21 Anti-Her2(T)-Fc1 light chain amino acid sequence SEQ ID NO: 22 Anti-Her2(P)-Fc1 heavy chain amino acid sequence SEQ ID NO: 23 Anti-Her2(P)-Fc1 light chain amino acid sequence SEQ ID NO: 24 Anti-PD-L1(Ate)-Fc1 heavy chain amino acid sequence SEQ ID NO: 25 D1-D2-D3-Fc2 DNA sequence SEQ ID NO: 26 D1-Fc2 amino acid sequence SEQ ID NO: 27 D1-D2-Fc2 amino acid sequence SEQ ID NO: 28 D1-D2-D3-Fc2 amino acid sequence SEQ ID NO: 29 D1.sup.m-Fc2 amino acid sequence SEQ ID NO: 30 D1 amino acid sequence SEQ ID NO: 31 D1-D2 amino acid sequence SEQ ID NO: 32 D1-D2-D3 amino acid sequence SEQ ID NO: 33 D1.sup.m amino acid sequence SEQ ID NO: 34 Ofa-Fc1 heavy chain DNA sequence SEQ ID NO: 35 Ofa-Fc1 light chain DNA sequence SEQ ID NO: 36 Obi-Fc1 heavy chain DNA sequence SEQ ID NO: 37 Anti-EGFR-Fc1 heavy chain DNA sequence SEQ ID NO: 38 Anti-Her2(T)-Fc1 heavy chain DNA sequence SEQ ID NO: 39 Anti-Her2(T)-Fc1 light chain DNA sequence SEQ ID NO: 40 Anti-Her2(P)-Fc1 heavy chain DNA sequence SEQ ID NO: 41 Anti-Her2(P)-Fc1 light chain DNA sequence SEQ ID NO: 42 Anti-PD-L1(Ate)-Fc1 heavy chain DNA sequence SEQ ID NO: 43 D1.sup.m-Fc2 DNA sequence SEQ ID NO: 44 D1.sup.m-D2-Fc2 amino acid sequence

(98) TABLE-US-00003 TABLE 2 Correspondence between the recombinant proteins and the sequences Protein name Sequence number in the sequence listing SIRPα D1-Fc SEQ ID NO: 11 Ofa-Fc1-D1-Fc2 SEQ ID NO: 16 (Ofa-Fc1 heavy chain) + SEQ ID NO: 17 (Ofa-Fc1 light chain) + SEQ ID NO: 26 (D1-Fc2) Ofa-Fc1-D1.sup.m-Fc2 SEQ ID NO: 16 (Ofa-Fc1 heavy chain) + SEQ ID NO: 17 (Ofa-Fc1 light chain) + SEQ ID NO: 29 (D1.sup.m-Fc2) Ofa-Fc1-D1-D2-Fc2 SEQ ID NO: 16 (Ofa-Fc1 heavy chain) + SEQ ID NO: 17 (Ofa-Fc1 light chain) + SEQ ID NO: 27 (D1-D2-Fc2) Ofa-Fc1-D1.sup.m-D2-Fc2 SEQ ID NO: 16 (Ofa-Fc1 heavy chain) + SEQ ID NO: 17 (Ofa-Fc1 light chain) + SEQ ID NO: 44 (D1.sup.m-D2-Fc2) Ofa-Fc1-D1-D2-D3-Fc2 SEQ ID NO: 16 (Ofa-Fc1 heavy chain) + SEQ ID NO: 17 (Ofa-Fc1 light chain) + SEQ ID NO: 28 (D1-D2-D3-Fc2) Obi-Fc1-D1-Fc2 SEQ ID NO: 18 (Obi-Fc1 heavy chain) + SEQ ID NO: 4 (Obi-Fc1 light chain) + SEQ ID NO: 26 (D1-Fc2) Anti-PD-L1(Ate)-Fc1-D1-Fc2 SEQ ID NO: 24 (Anti-PD-L1(Ate)-Fc1 heavy chain + SEQ ID NO: 10 (Anti-PD-L1(Ate)-Fc1 light chain) + SEQ ID NO: 26 (D1-Fc2) Anti-PD-L1(13G4)-Fc1-D1-Fc2 CN104356236A SEQ ID NO: 10 (Anti-PD-L1(13G4)-Fc1 heavy chain) + CN104356236A SEQ ID NO: 20 (Anti-PD-L1(13G4)-Fc1 light chain) + SEQ ID NO: 26 (D1-Fc2) Anti-PD-L1(12A4)-Fc1-D1-Fc2 CN104356236A SEQ ID NO: 2 (Anti-PD-L1(12A4)-Fc1 heavy chain) + CN104356236A SEQ ID NO: 12 (Anti-PD-L1(12A4)-Fc1 light chain) + SEQ ID NO: 26 (D1-Fc2) Anti-EGFR-Fc1-D1-Fc2 SEQ ID NO: 19 (Anti-EGFR-Fc1 heavy chain) + SEQ ID NO: 8 (Anti-EGFR-Fc1 light chain) + SEQ ID NO: 26 (D1-Fc2) Anti-Her2(T)-Fc1-D1-Fc2 SEQ ID NO: 20 (Anti-Her-2(T)-Fc1 heavy chain) + SEQ ID NO: 21 (Anti-Her2(T)-Fc1 light chain) + SEQ ID NO: 26 (D1-Fc2) Anti-Her2(P)-Fc1-D1-Fc2 SEQ ID NO: 22 (Anti-Her2(P)-Fc1 heavy chain) + SEQ ID NO: 23 (Anti-Her2(P)-Fc1 light chain) + SEQ ID NO: 26 (D1-Fc2) Obi-Fc1-D1.sup.m-Fc2 SEQ ID NO: 18 (Obi-Fc1 heavy chain) + SEQ ID NO: 4 (Obi-Fc1 light chain) + SEQ ID NO: 29 (D1.sup.m-Fc2) Anti-PD-L1(Ate)-Fc1-D1.sup.m-Fc2 SEQ ID NO: 24 (Anti-PD-L1(Ate)-Fc1 heavy chain) + SEQ ID NO: 10 (Anti-PD-L1(Ate)-Fc1 light chain) + SEQ ID NO: 29 (D1.sup.m-Fc2) Anti-PD-L1(13G4)-Fc1-D1.sup.m-Fc2 CN104356236A SEQ ID NO: 10 (Anti-PD-L1(13G4)-Fc1 heavy chain) + CN104356236A SEQ ID NO: 20 (Anti-PD-L1(13G4)-Fc1 light chain) + SEQ ID NO: 29 (D1.sup.m-Fc2) Anti-PD-L1(12A4)-Fc1-D1.sup.m-Fc2 CN104356236A SEQ ID NO: 2 (Anti-PD-L1(12A4)-Fc1 heavy chain) + CN104356236A SEQ ID NO: 12 (Anti-PD-L1(12A4)-Fc1 light chain) + SEQ ID NO: 29 (D1.sup.m-Fc2) Anti-EGFR-Fc1-D1.sup.m-Fc2 SEQ ID NO: 19 (Anti-EGFR-Fc1 heavy chain) + SEQ ID NO: 8 (Anti-EGFR-Fc1 light chain) + SEQ ID NO: 29 (D1.sup.m-Fc2) Anti-Her2(T)-Fc1-D1.sup.m-Fc2 SEQ ID NO: 20 (Anti-Her2(T)-Fc1 heavy chain) + SEQ ID NO: 21 (Anti-Her2(T)-Fc1 light chain) + SEQ ID NO: 29 (D1.sup.m-Fc2) Anti-Her2(P)-Fc1-D1.sup.m-Fc2 SEQ ID NO: 22 (Anti-Her2(P)-Fc1 heavy chain) + SEQ ID NO: 23 (Anti-Her2(P)-Fc1 light chain) + SEQ ID NO: 29 (D1.sup.m-Fc2) Obi-Fc1-D1-D2-Fc2 SEQ ID NO: 18 (Obi-Fc1 heavy chain) + SEQ ID NO: 4 (Obi-Fc1 light chain) + SEQ ID NO: 27 (D1-D2-Fc2) Anti-PD-L1(Ate)-Fc1-D1-D2-Fc2 SEQ ID NO: 24 (Anti-PD-L1(Ate)-Fc1 heavy chain) + SEQ ID NO: 10 (Anti-PD-L1(Ate)-Fc1 light chain) + SEQ ID NO: 27 (D1-D2-Fc2) Anti-PD-L1(13G4)-Fc1-D1-D2-Fc2 CN104356236A SEQ ID NO: 10 (Anti-PD-L1(13G4)-Fc1 heavy chain) + CN104356236A SEQ ID NO: 20 (Anti-PD-L1(13G4)-Fc1 light chain) + SEQ ID NO: 27 (D1-D2-Fc2) Anti-PD-L1(12A4)-Fc1-D1-D2-Fc2 CN104356236A SEQ ID NO: 2 (Anti-PD-L1(12A4)-Fc1 heavy chain) + CN104356236A SEQ ID NO: 12 (Anti-PD-L1(12A4)-Fc1 light chain) + SEQ ID NO: 27 (D1-D2-Fc2) Anti-EGFR-Fc1-D1-D2-Fc2 SEQ ID NO: 19 (Anti-EGFR-Fc1 heavy chain) + SEQ ID NO: 8 (Anti-EGFR-Fc1 light chain) + SEQ ID NO: 27 (D1-D2-Fc2) Anti-Her2(T)-Fc1-D1-D2-Fc2 SEQ ID NO: 20 (Anti-Her2(T)-Fc1 heavy chain) + SEQ ID NO: 21 (Anti-Her2(T)-Fc1 light chain) + SEQ ID NO: 27 (D1-D2-Fc2) Anti-Her2(P)-Fc1-D1-D2-Fc2 SEQ ID NO: 22 (Anti-Her2(P)-Fc1 heavy chain) + SEQ ID NO: 23 (Anti-Her2(P)-Fc1 light chain) + SEQ ID NO: 27 (D1-D2-Fc2) Obi-Fc1-D1.sup.m-D2-Fc2 SEQ ID NO: 18 (Obi-Fc1 heavy chain) + SEQ ID NO: 4 (Obi-Fc1 light chain) + SEQ ID NO: 44 (D1.sup.m-D2-Fc2) Anti-PD-L1(Ate)-Fc1-D1.sup.m-D2-Fc2 SEQ ID NO: 24 (Anti-PD-L1(Ate)-Fc1 heavy chain) + SEQ ID NO: 10 (Anti-PD-L1(Ate)-Fc1 light chain) + SEQ ID NO: 44 (D1.sup.m-D2-Fc2) Anti-PD-L1(13G4)-Fc1-D1.sup.m-D2-Fc2 CN104356236A SEQ ID NO: 10 (Anti-PD-L1(13G4)-Fc1 heavy chain) + CN104356236A SEQ ID NO: 20 (Anti-PD-L1(13G4)-Fc1 light chain) + SEQ ID NO: 44 (D1.sup.m-D2-Fc2) Anti-PD-L1(12A4)-Fc1-D1.sup.m-D2-Fc2 CN104356236A SEQ ID NO: 2 (Anti-PD-L1(12A4)-Fc1 heavy chain) + CN104356236A SEQ ID NO: 12 (Anti-PD-L1(12A4)-Fc1 light chain) + SEQ ID NO: 44 (D1.sup.m-D2-Fc2) Anti-EGFR-Fc1-D1.sup.m-D2-Fc2 SEQ ID NO: 19 (Anti-EGFR-Fc1 heavy chain) + SEQ ID NO: 8 (Anti-EGFR-Fc1 light chain) + SEQ ID NO: 44 (D1.sup.m-D2-Fc2) Anti-Her2(T)-Fc1-D1.sup.m-D2-Fc2 SEQ ID NO: 20 (Anti-Her2(T)-Fc1 heavy chain) + SEQ ID NO: 21(Anti-Her2(T)-Fc1 light chain) + SEQ ID NO: 44 (D1.sup.m-D2-Fc2) Anti-Her2(P)-Fc1-D1.sup.m-D2-Fc2 SEQ ID NO: 22 (Anti-Her2(P)-Fc1 heavy chain) + SEQ ID NO: 23 (Anti-Her2(P)-Fc1 light chain) + SEQ ID 44 (D1.sup.m-D2-Fc2) Obi-Fc1-D1-D2-D3-Fc2 SEQ ID NO: 18 (Obi-Fc1 heavy chain) + SEQ ID NO: 4 (Obi-Fc1 light chain) + SEQ ID NO: 28 (D1-D2-D3-Fc2) Anti-PD-L1(Ate)-Fc1-D1-D2-D3-Fc2 SEQ ID NO: 24 (Anti-PD-L1(Ate)-Fc1 heavy chain) + SEQ ID NO: 10 (Anti-PD-L1(Ate)-Fc1 light chain) + SEQ ID NO: 28 (D1-D2-D3-Fc2) Anti-PD-L1(13G4)-Fc1-D1-D2-D3-Fc2 CN104356236A SEQ ID NO: 10 (Anti-PD-L1(13G4)-Fc1 heavy chain) + CN104356236A SEQ ID NO: 20 (Anti-PD-L1(13G4)-Fc1 light chain) + SEQ ID NO: 28 (D1-D2-D3-Fc2) Anti-PD-L1(12A4)-Fc1-D1-D2-D3-Fc2 CN104356236A SEQ ID NO: 2 (Anti-PD-L1(12A4)-Fc1 heavy chain) + CN104356236A SEQ ID NO: 12 (Anti-PD-L1(12A4)-Fc1 light chain) + SEQ ID NO: 28 (D1-D2-D3-Fc2) Anti-EGFR-Fc1-D1-D2-D3-Fc2 SEQ ID NO: 19 (Anti-EGFR-Fc1 heavy chain) + SEQ ID NO: 8 (Anti-EGFR-Fc1 light chain) + SEQ ID NO: 28 (D1-D2-D3-Fc2) Anti-Her2(T)-Fc1-D1-D2-D3-Fc2 SEQ ID NO: 20 (Anti-Her2(T)-Fc1 heavy chain) + SEQ ID NO: 21 (Anti-Her2(T)-Fc1 light chain) + SEQ ID NO: 28 (D1-D2-D3-Fc2) Anti-Her2(P)-Fc1-D1-D2-D3-Fc2 SEQ ID NO: 22 (Anti-Her2(P)-Fc1 heavy chain) + SEQ ID NO: 23 (Anti-Her2(P)-Fc1 light chain) + SEQ ID NO: 28 (D1-D2-D3-Fc2)

EXAMPLE 1

Construction of Expression Vector

(99) Basing on the designed molecular structure, the amino acid sequences of each component were spliced together. According to the preference of Chinese hamsters (Cricetulus griseus) for codons, an optimal DNA coding sequence was designed and endonuclease restriction recognition sites for later use in gene cloning operation were excluded. Then a cloning site, Kozak sequence and a signal peptide coding sequence were added successively at the 5′ end of the sequence, and a stop codon and a cloning site were added successively at the 3′ end of the sequence, as shown in FIG. 1.

(100) Whole gene synthesis was performed, and the whole gene was directionally cloned between the corresponding cloning sites of expression vector pCHO-TE2 (purchased from Thermo Fisher) using the 5′ end and 3′ end cloning sites. After the correctness of the sequence was verified, the expression plasmid was obtained. All the cloning sites used at 5′ end and 3′ end are EcoRV and PacI sites respectively. FIG. 2 was a plasmid map of the expression vector pCHO-TE2.

EXAMPLE 2

Preparation of Expression Plasmid, Cell Transfection, and Expression and Purification of Target Protein

(101) Preparation of Expression Plasmid

(102) A bacterial glycerol stock containing the expression plasmid (1 mL of a solution of Eseherichia coil containing the expression plasmid was thoroughly mixed with 0.5 mL of 60% sterilized glycerin solution) was inoculated into a liquid LB medium at a ratio of 1:1000. After 16 hours of culture in a shaker at 37° C., 22.0 rpm, the bacteria were collected by centrifugation. The expression plasmid was obtained by using endotoxin-free plasmid prep kits (DP117, purchased from Tiangen Biotech (Beijing) Co., Ltd.) according to the standard procedure provided by kit instructions.

(103) Cell Transfection and Protein Expression

(104) After the obtained expression plasmid was filtered through a 0.22 μm microfiltration membrane, 3 mg of the plasmid solution (wherein the product was a typical antibody molecule, and the ratio of the light chain and the heavy chain expression plasmids were 1:1 (molar ratio); wherein the product was a recombinant protein, the ratio of the light chain, the heavy chain and the right arm expression plasmids were 1:1:1 (molar ratio), as shown in Table 3) was pipetted into 50 mL Opti MEM I Reduced Serum Medium (purchased from GIBCO), then mixed thoroughly. 6 mg of transfection reagent polyetherimide (PEI, purchased from Polysciences, dissolved in sterile ultrapure water at a concentration of 1 mg/mL) was transferred into 50 mL Opti MEM I Reduced Serum Medium, then mixed thoroughly. The obtained PEI solution was added to the Opti MEM I Reduced Serum Medium solution containing the plasmid and mixed thoroughly. The mixture of the plasmid and PEI was allowed to stand at room temperature for 15 minutes, and then slowly and evenly added into 1 L of a suspension of host cell CHO-S (purchased from Thermo Fisher) with a cell density of 3×10.sup.6 cells/mL. The cells was cultured in an incubator containing 5% CO.sub.2 at 37° C. 4 hours later, a feed medium (the feed medium was prepared by dissolving 80 g of CD Efficient Feed C AGT (purchased from Gibco) and 75 g of 5×00483 (purchased from Kerry) in 1 L of water) of a volume equivalent to 7% of the initial volume was added therein. The culture temperature was lowered to 33° C. and the cells were harvested upon 6 days of culture: The cell suspension was centrifuged at 10,000 g, 10° C. for 30 minutes, and the supernatant (i.e., the cell culture harvest solution) was used for purification of the target protein.

(105) Protein Purification

(106) The following method takes Ofa-Fc1-D1-Fc2 as an example, using protein A for affinity-capture of the product.

(107) The above cell culture harvest solution was centrifuged at 10,000 rpm for 30 min to remove the cells and fragments thereof, then loaded onto a protein A affinity column (Art No. 17-5438-02, GE Healthcare), and eluted to harvest the target protein. The purity of the target protein was determined by SDS-PAGE.

(108) The protein A purification method is a conventional protein purification method well known to those skilled in the art, and the detailed test procedure can refer to the product description of GE Healthcare Protein A and the GE antibody purification handbook.

(109) The theoretical molecular weights of the four proteins SIRPα D1-Fc, Ofa-Fc1-D1-Fc2, Ofa-Fc1-D1-D2-Fc2, and Ofa-Fc1-D1-D2-D3-Fc2 are 37.8 kD, 110.7 kD, 121.7 kD and 131.4 kD, respectively. The results of SDS-PAGE are shown in FIG. 4A and FIG. 4B.

(110) Protein electrophoresis (SDS-PAGE): The results show (FIG. 4A and FIG. 4B) that the target proteins in each lane are efficiently expressed and purified, wherein Ofa-Fc1-D1-Fc2 (FIG. 4A, lane 11), Ofa-Fc1-D1-D2-Fc2 (FIG. 4A, lane 1) and Ofa-Fc1-D1-D2-D3-Fc2 (FIG. 4A, lane 12) shows different degrees of left arm dialer (Ofa-Fc1-Ofa-Fc1), right arm dimer (SIRPα-Fc2) and/or multimer.

(111) TABLE-US-00004 TABLE 3 Ratio of the expression plasmid Product Ratio of the expression plasmid Typical Anti-CD20 mAb (Ofatumumab) the ratio of the light antibody Anti-CD20 mAb (Obinutuzumab) chain and the heavy chain molecules/ Anti-EGFR mAb (JMT101) expression plasmids were fusion Anti-CD47 mAb (Hu5F9-G4) 1:1 protein Anti-Her2(T) mAb (Transtuzumab) dimer Anti-Her2(P) mAb (Pertuzumab) Anti-PD-L1mAb (Atezolizumab) Anti-PD-L1mAb(12A4) Anti-PD-L1mAb (13G4) SIRPα D1-Fc Dimer Recombinant Ofa-Fc1-D1-Fc2 the ratio of the expression proteins Ofa-Fc1-D1.sup.m-Fc2 plasmids of light chain, Ofa-Fc1-D1-D2-Fc2 heavy chain and right arm Ofa-Fc1-D1.sup.m-D2-Fc2 follows the aforementioned Ofa-Fc1-D1-D2-D3-Fc2 ratio Obi-Fc1-D1-Fc2 Anti-PD-L1(Ate)-Fc1-D1-Fc2 Anti-PD-L1(13G4)-Fc1-D1-Fc2 Anti-PD-L1(12A4)-Fc1-D1-Fc2 Anti-EGFR-Fc1-D1-Fc2 Anti-Her2(T)-Fc1-D1-Fc2 Anti-Her2(P)-Fc1-D1-Fc2 Obi-Fc1-D1.sup.m-Fc2 Anti-PD-L1(Ate)-Fc1-D1.sup.m -Fc2 Anti-PD-L1(13G4)-Fc1-D1.sup.m -Fc2 Anti-PD-L1(12A4)-Fc1-D1.sup.m -Fc2 Anti-EGFR-Fc1-D1.sup.m-Fc2 Anti-Her2(T)-Fc1-D1.sup.m-Fc2 Anti-Her2(P)-Fc1-D1.sup.m-Fc2 Obi-Fc1-D1-D2-Fc2 Anti-PD-L1(Ate)-Fc1-D1-D2-Fc2 Anti-PD-L1(13G4)-Fc1-D1-D2-Fc2 Anti-PD-L1(12A4)-Fc1-D1-D2-Fc2 Anti-EGFR-Fc1-D1-D2-Fc2 Anti-Her2(T)-Fc1-D1-D2-Fc2 Anti-Her2(P)-Fc1-D1-D2-Fc2 Obi-Fc1-D1.sup.m-D2-Fc2 Anti-PD-L1(Ate)-Fc1-D1.sup.m-D2-Fc2 Anti-PD-L1(13G4)-Fc1-D1.sup.m-D2-Fc2 Anti-PD-L1(12A4)-Fc1-D1.sup.m-D2-Fc2 Anti-EGFR-Fc1-D1.sup.m-D2-Fc2 Anti-Her2(T)-Fc1-D1.sup.m-D2-Fc2 Anti-Her2(P)-Fc1-D1.sup.m-D2-Fc2 Obi-Fc1-D1-D2-D3-Fc2 Anti-PD-L1(Ate)-Fc1-D1-D2-D3-Fc2 Anti-PD-L1(13G4)-Fc1-D1-D2-D3-Fc2 Anti-PD-L1(12A4)-Fc1-D1-D2-D3-Fc2 Anti-EGFR-Fc1-D1-D2-D3-Fc2 Anti-Her2(T)-Fc1-D1-D2-D3-Fc2 Anti-Her2(P)-Fc1-D1-D2-D3-Fc2 Notes: D1.sup.mrepresents a high affinity mutant of SIRPα extracellular truncated variantD1; D1 represents the extracellular D1 domain of human wild-type SIRPα and its non-high affinity mutant; Fc is a wild-type Fc region; Fc1 is an Fc region having a hole or holes mutation(s), and Fc2 is an Fc region having a knob or knobs mutation(s).

EXAMPLE 3

Determination of Affinity, Competitive Binding Activity to the Target

(112) 1. Detection method for affinity of the target CD47, CD20, EGFR and Her2

(113) The binding affinity of the recombinant proteins Ofa-Fc1-D1-Fc2, Ofa-Fc1-D1.sup.m-Fc2, Ofa-Fc1-Dc1-D2-Fc2, Ofa-Fc1-D1.sup.m-D2-Fc2, Ofa-Fc1-D1-D2-D3-Fc2, Obi-Fc1-D1-Fc2, Obi-Fc1-D1.sup.m-Fc2, Obi-Fc1-D1-D2-Fc2, Obi-Fc1-D1.sup.m-D2-Fc and Obi-Fc1-D1-D2-D3-Fc2 to the targets CD47 and CD20 was determined by ELISA and/or flow cytometry. Taking Ofa-Fc1-D1-Fc2 as an example, the following detect method is suitable for the recombinant proteins with a left arm targeting CD20.

(114) The binding affinity of the recombinant proteins Anti-Her2(P)-Fc1-D1-Fc2, Anti-Her2(P)-Fc1-D1.sup.m-Fc2, Anti-Her2(P)-Fc1-D1-D2-Fc2, Anti-Her2(P)-Fc1-D1.sup.m-D2-Fc2, Anti-Her2(P)-Fc1-D1-D2-D3-Fc2, Anti-Her2(P)-Fc1-D1-Fc2, Anti-Her2(P)-Fc1-D1.sup.m-Fc2, Anti-Her2(P)-Fc1-D1-D2-Ec2, Anti-Her2(P)-Fc1-D1.sup.m-D2-Fc2, Anti-Her2(P)-Fc1-D1-D2-D3-Fc2, Anti-Her2(T)-Fc1-D1-Fc2, Anti-Her2(T)-Fc1-D1.sup.m-Fc2, Anti-Her2(T)-Fc1-D1-D2-Fc2, Anti-Her2(T)-Fc1-D1.sup.m-D2-Fc2, Anti-Her2(T)-Fc1-D1-D2-D3-Fc2, Anti-Her2(T)-Fc1-D1-Fc2, Anti-Her2(T)-Fc1-D1.sup.m-Fc2, Anti-Her2(T)-Fc1-D1-D2-Fc2, Anti-Her2(T)-Fc1-D1.sup.m-D2-Fc2 and Anti-Her2(T)-Fc1-D1-D2-D3-Fc2 to the targets CD47 and Her2 was determined by ELISA and/or flow cytometry. Taking Anti-Her2(T)-Fc1-D1-Fc2 as an example, the following detect method is suitable for the recombinant proteins with a left arm targeting Her2.

(115) The binding affinity of the recombinant proteins Anti-EGFR-Fc1-D1-Fe2, Anti-EGFR-Fc1-D1.sup.m-Fc2, Anti-EGFR-Fc1-D1-D2-Fc2, Anti-EGFR-Fc1-D1.sup.m-D2-Fc2 and Anti-EGFR-Fc1-D1-D2-D3-Fc2 to the targets CD47 and EGFR was determined by ELISA and/or flow cytometry. Taking Anti-EGFR-Fc1-D1-Fc as an example, the following detect method is suitable for the recombinant proteins with a left arm targeting EGFR.

(116) The binding affinity of the recombinant proteins Anti-PD-L1(Ate)-Fc1-D1-Fc2, Anti-PD-L1(Ate)-Fc1-D1.sup.m-Fc2, Anti-PD-L1(Ate)-Fc1-D1-D2-Fc2, Anti-PD-L1(Ate)-Fc1-D1.sup.m-D2-Fc2 Anti-PD-L1(Ate)-Fc1-D1-D2-D3-Fc2, Anti-PD-L1(13G4)-Fc1-D1-Fc2, Anti-PD-L1(13G4)-Fc1-D1.sup.m-Fc2, Anti-PD-L1(13G4)-Fc1-D1-D2-Fc2, Anti-PD-L1(13G4)-Fc1-D1.sup.m-D2-Fc2, Anti-PD-L1(13G4)-Fc1-D1-D2-D3-Fc2, Anti-PD-L1(12A4)-Fc1-D1-Fc2, Anti-PD-L1(12A4)-Fc1-D1.sup.m-Fc2, Anti-PD-L1(12A4)-Fc1-D1-D2-Fc2, Anti-PD-L1(12A4)-Fc1-D1.sup.m-D2-Fc2 and Anti-PD-L1(12A4)-Fc1-D1-D2-D3-Fc2 to the targets CD47 and PD-L1 was determined by ELISA. Taking Anti-PD-L1(Ate)-Fc1-D1-Fc2 as an example, the following detect method is suitable for the recombinant proteins with a left arm targeting PD-L1.

(117) Determination of the Affinity of Ofa-Fc1-D1-Fc2 and Anti-EGFR-Fc1-D1-Fc2 to the target CD47 by ELISA:

(118) An ELISA plate (Art No. 9018, Corning) was coated with 100 μL of 1 μg/mL CD47-His (12283-H08H-200, Sino Biological) and placed at 4° C. overnight. The plate was rinsed with PBST solution (PBS containing 0.1% Tweet 20), and then blocked with PBS+1% BSA for 2 hours at room temperature. After rinsing, 100 μL of diluted Ofa-Fc1-D1-Fc2 and Anti-EGFR-Fc1-D1-Fc2 (2,5-fold serial dilutions starting from 1000 ng/mL, 11 dilutions) was added to each well of the coated plate, then incubated for 1 hour at 25° C. After discarding the sample and rinsing the plate three times with PBST solution, 100 μL of diluted mouse anti-human IgG Fc-HRP (1:10000) (Ab7499, abeam) was added, then incubated at 25° C. for 1 hour. After discarding the solution and rinsing the plate three times with PBST solution, TMB (P0209, beyotime) was added, and the plate was developed and protected from light for about 20 minutes. The reaction was stopped with H.sub.2SO.sub.4, and the OD value at 450-650 nm was read on a microplate reader.

(119) The test results showed that anti-CD47 antibodies Hu5F9-G4, SIRPα D1-Fc, Ofa-Fc1-D1-Fc2, Ofa-Fc1-D1.sup.m-Fc2, Ofa-Fc1-D1-D2-Fc2, Ofa-Fc1-D1.sup.m-D2-Fc2, Ofa-Fc1-D1-D2-D3-Fc2, Obi-Fc1-D1-Fc2, Obi-Fc1-D1.sup.m-Fc2, Obi-Fc1-D1-D2-Fc2, Obi-Fc1-D1.sup.m-D1-D2-Fc2, Obi-Fc1-D1-D2-D3-Fc2, Anti-EGFR-Fc1-D1-Fc2, Anti-EGFR-Fc1-D1.sup.m-Fc2, Anti-EGFR-Fc1-D1-D2-Fc2, Anti-EGFR-Fc1-D1.sup.m-D2-Fc2, Anti-EGFR-Fc1-D1-D2-D3-Fc2 were all capable of binding to CD47; the binding affinity of Ofa-Fc1-D1-Fc2, Ofa-Fc1-D1-D2-Fc2, Ofa-Fc1-D1-D2-D3-Fc2, Obi-Fc1-D1-Fc2, Obi-Fc1-D1-D2-Fc2, Obi-Fc1-D1-D2-D3-Fc2, Anti-EGFR-Fc1-D1-Fc2, Anti-EGFR-Fc1-D1-D2-Fc2, Anti-EGFR-Fc1-D1-D2-D3-Fc2 to CD47 was slightly weaker than the binding affinity of anti-CD47 antibody Hu5F9-64 and/or SIRPα D1-Fc to CD47.

(120) The above test results demonstrate that the recombinant proteins of the present disclosure are capable of specifically targeting the CD47 antigen on tumor cells at the protein level, and their binding affinity to CD47 is not higher than the binding affinity of SIRPα D1-Fc fusion protein to CD47. The recombinant proteins of the present disclosure can reduce or avoid the side effects such as RBC agglutination, anemia caused by the treatment with anti-CD47 antibody and/or killing of non-tumor target cells caused by the treatment with high affinity SIRPα, mutant (Petrova P S, et al, TTI-621 (SIRPα Fc): A CD47-Blocking Innate Immune Checkpoint Inhibitor with Broad Antitumor Activity and Minimal Erythrocyte Binding. Clin Cancer Res, 2017, 23(4): 1068-1079).

(121) For example, as shown in FIG. 5, except that the anti-CD20 antibody Ofatumumab and the anti-EGFR antibody JMT101 cannot bind to CD47, anti-CD47 antibodies Hu5F9-G4, SIRPα D1-Fc, OFa-Fc1-D1-Fc2, Anti-EGFR-Fc1-D1-Fc2 are all capable of binding to CD47. However, the binding affinity of Ofa-Fc1-D1-Fc2 and Anti-EGFR-Fc1-D1-Fc2 (EC.sub.50=52.57 ng/mL, EC.sub.50=93.86 ng/mL), of which only the right arm can bind to CD47 antigen, is weaker than that of anti-CD47 antibody (EC.sub.50=5.439 ng/mL) and SIRPα D1-Fc (EC50=6.118 ng/mL).

(122) Determination of Affinity of Ofa-Fc1-D1-Fc2 to the Target CD47 by ELISA Flow Cytometry

(123) Well-grown A431 cells (human epidermal cancer cell) were collected and counted, centrifuged and resuspended to a concentration of 3×10.sup.6 cells/mL with PBS+2% FBS (purchased from Gibco). 100 μL of the cell suspension was aliquoted to each well of a 96-well U-plate (Art No. 3799, Corning) and allowed to stand for at least 15 minutes. The supernatant was pipetted and discarded after centrifugation, then 9 serial dilutions of Ofa-Fc1-D1-Fc2 were added (5-fold serial dilutions starting from 15000 ng/mL, a total of 9 concentrations) and incubated in a refrigerator at 4° C. for 1 hour. After rinsing with PBS+2% FBS, goat anti-human IgG Fc-FITC (F9512-2ML, Sigma) was added and incubated for 1 hour at 4° C. After rinsing and resuspension with PBS+2% FBS, the fluorescence value was determined by a flow cytometer (Accuri C6, BD).

(124) Since A431 cells do not express CD20 antigen and cannot bind to Ofatumumab and Obinutuzumab, A431 cells can be used to evaluate the binding affinity of Ofa-Fc1-D1-Fc2, Ofa-Fc1-D1.sup.m-Fc2, Ofa-Fc1-D1-D2-Fc2, Ofa-Fc1-D1.sup.m-D2-Fc2, Ofa-Fc1-D1-D2-D3-Fc2, Obi-Fc1-D1-Fc2, Obi-Fc1-D1-D2-Fc2, Obi-Fc1-D1.sup.m-D2-Fc2 and Obi-Fc1-D1-D2-D3-Fc to CD47 at the cellular level.

(125) The test results showed, except that the anti-CD20 antibodies Ofatumumab and Obinutuzumab cannot bind to A431 cells, anti-CD47 antibodies Hu5F9-G4, SIRPα D1-Fc, Ofa-Fc1-D1-Fc2, Ofa-Fc1-D1.sup.m-Fc2, Ofa-Fc1-D1-D2-Fc2, Ofa-Fc1-D1.sup.m-D2-Fc2, Ofa-Fc1-D1-D2-D3-Fc2, Obi-Fc1-D1-Fc2, Obi-Fc1-D1.sup.m-Fc2, Obi-Fc1-D1-D2-Fc2, Obi-Fc1-D1.sup.m-D2-Fc2 and Obi-Fc1-D1-D2-D3-Fc2 were all capable of binding to A431 cells. The binding affinity of Ofa-Fc1-D1-Fc2, Ofa-Fc1-D1-D2-Fc2, Ofa-Fc1-D1-D2-D3-Fc2, Obi-Fc1-D1-Fc2, Obi-Fc1-D1-D2-Fc2 and Obi-Fc1-D1-D2-D3-Fc2 to CD47 was slightly weaker than the binding affinity of anti-CD47 antibody and/or SIRPα D1-Fc to CD47, which was consistent with the trend of ELISA data.

(126) The above test results demonstrate that the recombinant proteins of the present disclosure are capable of specifically targeting the CD47 antigen on tumor cells at the protein level, and the binding affinity to CD47 is not higher than the binding affinity of SIRPα D1-Fc fusion protein to CD47. The recombinant proteins of the present disclosure can reduce or avoid the side effects such as RBC agglutination, anemia caused by the treatment with anti-CD47 antibody and/or killing of non-tumor target cells caused by the treatment with high affinity SIRPα mutant.

(127) For example, as shown in FIG. 6, anti-CD47 antibodies Hu5F9-G4, SIRPα D1-Fc and Ofa-Fc1-D1-Fc2 are all capable of binding to A431 cells. Specifically, the binding affinity of Ofa-Fc1-D1-Fc2 is slightly weaker than that of the anti-CD47 antibody and/or SIRPα D1-Fc, which is consistent with the trend of ELISA data.

(128) Determination of Affinity of Ofa-Fc1-D1-Fc2 to the target CD20 by Flow Cytometry

(129) Well-grown Raji cells (human B-cell lymphoma, purchased from the Cell Bank of Chinese Academy of Sciences, Shanghai) were collected and counted, centrifuged and resuspended to a concentration of 3×10.sup.6 cells/MI, with PBS+2% FBS. 100 μL of the cell suspension was aliquoted to each of a 96-well U-plate (Art No. 3799, Corning) and allowed to stand for at least 15 minutes. The supernatant was pipetted and discarded after centrifugation, then 100 μL, PBS+2% FBS (control group) or 1.5 μg/mL, anti-CD47 antibody Hu5F9-G4 (Fab)2 (treatment group) (excising Fc by pepsin, kit: Thermo Fisher, 44988) were added and incubated at 4° C. for 1 hour. After rinsing with PBS+2% FBS, 7 serial dilutions of Ofa-Fc1-D1-Fc2, Hu5F9-G4 or SIRPα D1-Fc (4-fold serial dilutions starting from 6250 ng/mL, with a total of 7 dilutions, and the molar concentration after conversion is shown in FIG. 7) were added respectively and then incubated at 4° C. for 1 hour. After rinsing with PBS+2% FBS, goat anti-human IgG Fc-FITC (F9512-2ML, Sigma) was added and incubated for 1 hour at 4° C. After rinsing and resuspension with PBS+2% FBS, the fluorescence value was determined by a flow cytometer (Accuri C6, BD).

(130) The test results showed that the anti-CD47 antibody Hu5F9-G4(Fab)2 effectively blocked the binding of the anti-CD47 antibody Hu5F9-G4 and/or SIRPα D1-Fc to CD47 on Raji cells. However, the blocking effect of Hu5F9-G4(Fab)2 on CD47 antigen did not significantly inhibit the binding of Ofa-Fc1-D1-Fc2, Ofa-Fc1-D1-D2-Fc2, Ofa-Fc1-D1-D2-D3-Fc2, Obi-Fc1-D1-Fc2, Obi Fc1-D1-D2-Fc2 and Obi-Fc1-D1-D2-D3-Fc2 to Raji cells.

(131) The above test results demonstrate that, in the case where the CD47 antigen on the surface of tumor cells is shielded and the binding to SIRPα-CD47 is blocked, the recombinant proteins of the present disclosure are still capable of specifically binding to the corresponding antigen on tumor cells by the left arm, and the affinity of the left arm is not significantly affected by blocking the binding of the right arm.

(132) For example, as shown in FIG. 7, the anti-CD47 antibody Hu5F9-G4(Fab)2 effectively blocks the binding of the anti-CD47 antibody Hu5F9-G4 and/or SIRPα D1-Fc to CD47 on Raji cells. However, the blocking effect of Hu5F9-G4(Fab)2 on CD47 antigen does not significantly inhibit the binding of Ofa-Fc1-D1-Fc2 to Raji cells, indicating that Ofa-Fc1-D1-Fc2 is still capable of specifically binding to the CD20 antigen on Raji cells by its left arm (anti-CD20 half antibody) after the mutual combination of SIRPα-CD47 is blocked, and the affinity is not significantly affected by blocking the binding of the right arm.

(133) Determination of Bispecific Binding Activity to the Targets CD20 and CD47 by Flow Cytometry

(134) Well-grown Raji cells (human B-cell lymphoma, purchased from the Cell Bank of Chinese Academy of Sciences, Shanghai) were collected and counted, centrifuged and resuspended to a concentration of 3×10.sup.6 cells/mL with PBS+2% FBS. 100 μL of the cell suspension was aliquoted to each well of a 96-well U-plate (Art No. 3799, Corning) and allowed to stand for at least 15 minutes. The supernatant was pipetted and discarded after centrifugation, then 12 serial dilutions of Ofa-Fc1-D1-Fc2, Ofatumumab, Hu5F9-G4 or SIRPα D1-Fc (50000 ng/mL, 25000 ng/mL, 6250 ng/mL and 4-fold serial dilutions starting from 6250 ng/mL, with a total of 12 dilutions, and the molar concentration after conversion is shown in FIG. 8A) were added respectively and incubated at 4° C. for 1 hour. After rinsing with PBS+2% FBS, goat anti-human IgG Fc-FITC (F9512-2ML, Sigma) was added and incubated for 1 hour at 4° C. After rinsing and resuspension with PBS+2% FBS, the fluorescence value was determined by a flow cytometer (Accuri C6, BD).

(135) Since the surface of Raji cells simultaneously expresses CD20 and CD47 antigens, the anti-CD20 antibodies Ofatumumab, Obinutuzumab, anti-CD47 antibody Hu5F9-G4 and SIRPα D1-Fc are all capable of specifically binding to Raji cells, but their maximum average fluorescence intensities are not identical to each other.

(136) The test results showed that Ofa-Fc1-D1-Fc2, Ofa-Fc1-D1.sup.m-Fc2, Ofa-Fc1-D1-D2-Fc2, Ofa-Fc1-D1.sup.m-D2-Fc2, Ofa-Fc1-D1-D2-D3-Fc2, Obi-Fc1-D1-Fc2, Obi-Fc1-D1.sup.m-Fc2, Obi-Fc1-D1-D2-Fc2, Obi-Fc1-D1.sup.m-D2-Fc2 and Obi-Fc1-D1-D2-D3-Fc2 were capable of binding to Raji cells, and achieved a higher maximum average fluorescence intensity.

(137) The above test results demonstrate that the recombinant proteins of the present disclosure, compared with the anti-CD20 antibodies Ofatumumab, Obinutuzumab and/or the anti-CD47 antibody Hu5F9-G4 and/or SIRPα D1-Fc, are capable of specifically binding to tumor cells and exhibit a significant advantage in the number of molecules under the environment of a same supersaturated protein concentration. Preferably, the saturation binding abundance of the recombinant proteins of the present disclosure to tumor cells is greater than the sum of the saturation binding abundance of the anti-CD20 antibody and SIRPα D1-Fc to tumor cells under the environment of a same supersaturated protein concentration.

(138) TABLE-US-00005 TABLE 4 Maximum average fluorescence intensity and EC.sub.50 (nM) of the binding of antibodies/recombinant proteins to Raji cells Sample Ofa-Fc1- SIRPα Ofatumumab D1-Fc2 Hu5F9-G4 D1-Fc Maximum 252065.10 690705.90 93784.59 104997.50 average fluorescence intensity EC.sub.50 (nM) 1.586 14.06 0.258 12.53

(139) For example, as shown in FIG. 8A and Table 4, the anti-CD20 antibody Ofatumumab, the anti-CD47 antibody Hu5F9-G4 and SIRPα D1-Fc are all capable of specifically binding to Raji cells, but their maximum average fluorescence intensities are various; meanwhile, Ofa-Fc1-D1-Fc2 is also capable of binding to Raji cells and has a higher maximum average fluorescence intensity, indicating that under the condition of a same supersaturated protein concentration, the number of Ofa-Fc1-D1-Fc2 molecules specifically binding to Raji cells is significantly greater than that of the anti-CD20 antibody Ofatumumab or the anti-CD47 antibody Hu5F9-G4 or SIRPα D1-Fc molecules, greater than the sum of the anti-CD20 antibody Ofatumab and the anti-CD47 antibody Hu5F9-G4 molecules, and greater than the sum of the antibody Ofatumumab and SIRPα D1-Fc molecules.

(140) As known to those skilled in the art, the above test results indicate that the recombinant proteins of the present disclosure which are capable of simultaneously binding to both the tumor-targeting antigen and CD47 can bind more to tumor cells and thus provide a more significant anti-tumor effect, compared with the combination therapy of the anti-CD47 antibody or SIRPα D1-Fc with another tumor-targeting therapeutic antibody.

(141) Determination of Bispecific Binding Activity to the Targets Her2 and CD47 by Flow Cytometry

(142) Well-grown SKBR-3 cells (human breast cancer cell, purchased from the Cell Bank of Chinese Academy of Sciences, Shanghai) were collected and counted, centrifuged and resuspended to a concentration of 2×10.sup.6 cells/mL with PBS+2% FBS. 100 μL of the cell suspension was aliquoted to each well of a 96-well U-plate (Art No. 3799, Corning) and allowed to stand for at least 15 minutes. The supernatant was pipetted and discarded after centrifugation, then 10 dilutions of Anti-Her2(T)-Fc1-D1-Fc2, Trastuzumab or Hu5F9-G4 (4-fold serial dilutions starting from 433.2 nM, a total of 10 dilutions) were added respectively and incubated at 4° C. for 1 hour. After rinsing with PBS+2% FBS, goat anti-human IgG Fc-FITC (F9512-2ML, Sigma) was added and incubated for 1 hour at 4° C. After rinsing and resuspension with PBS+2% FBS, the fluorescence value was determined by a flow cytometer (Accuri C6, BD).

(143) Since the surface of SKBR-3 cells simultaneously expresses Her2 and CD47 antigens, the anti-Her2 antibodies Trastuzumab, Pertuzumab, the anti-CD47 antibody. Hu5F9-G4 and SIRPα D1-Fc are all capable of specifically binding to SKBR-3 cells, but their maximum average fluorescence intensities are various.

(144) The test results showed that Anti-Her2(P)-Fc1-D1-Fc2, Anti-Her2(P)-Fc1-D1.sup.m-Fc2, Anti-Her2(P)-Fc1-D1-D2-Fc2, Anti-Her2(P)-Fc1-D1.sup.m-D2-Fc2, Anti-Her2(P)-Fc1-D1-D2-D3-Fc2, Anti-Her2(P)-Fc1-D1-Fc2, Anti-Her2(P)-Fc1-D1.sup.m-Fc2, Anti-Her2(P)-Fc1-D1-D2-Fc2, Anti-Her2(P)-Fc1-D1.sup.m-D2-Fc2, Anti-Her2(P)-Fc1-D1-D2-D3-Fc2, Anti-Her2(T)-Fc1-D1-Fc2, Anti-Her2(T)-Fc1-D1.sup.m-Fc2, Anti-Her2(T)-Fc1-D1-D2-Fc2, Anti-Her2(T)-Fc1-D1.sup.m-D2-Fc2, Anti-Her2(T)-Fc1-D1-D2-D3-Fc2, Anti-Her2(T)-Fc1-D1-Fc2, Anti-Her2(T)-Fc1-D1.sup.m-Fc2, Anti-Her2(T)-Fc1-D1-D2-Fc2, Anti-Her2(T)-Fc1-D1.sup.m-D2-Fc2 and Anti-Her2(T)-Fc1-D1-D2-D3-Fc2 were capable of binding to SKBR-3 cells, and achieved a higher maximum average fluorescence intensity.

(145) The above test results demonstrate that the recombinant proteins of the present disclosure, are capable of specifically binding to tumor cells and exhibit a significant advantage in the number of molecules under the condition of a same supersaturated protein concentration, compared with the anti-Her2 antibodies Trastuzumab, Pertuzumab and/or the anti-CD47 antibody Hu5F9-G4 and/or SIRPα D1-Fc. Preferably, the saturation binding abundance of the recombinant proteins of the present disclosure to tumor cells is greater than the sum of the saturation binding abundance of the anti-Her2 antibody and SIRPα D1-Fc to tumor cells under the environment of a same supersaturated protein concentration.

(146) TABLE-US-00006 TABLE 5 Maximum average fluorescence intensity and EC.sub.50 (nM) of the binding of antibodies/recombinant proteins to SKBR-3 cells Sample Anti-Her2(T)-Fc1-D1-Fc2 Hu5F9-G4 Trastuzumab Maximum 3465348.00 213251.50 1841783.00 average fluorescence intensity EC50 (nM) 10.03 0.4557 3.204

(147) For example, as shown in FIG. 8B and Table 5, the anti-Her2 antibody Trastuzumab and the anti-CD47 antibody Hu5F9-G4 are both capable of specifically binding to SKBR-3 cells, but their maximum average fluorescence intensities are not identical to each other; meanwhile, Anti-Her2(T)-Fc1-D1-Fc2 is also capable of binding to SKBR-3 cells and has a higher maximum average fluorescence intensity, indicating that under the enviromnent of a same supersaturated protein concentration, the number of Anti-Her2(T)-Fc1-D1-Fc2 molecules specifically binding to SKBR-3 cells is significantly greater than that of the anti-Her2 antibody Trastuzumab or the anti-CD47 antibody Hu5F9-G4 molecules, and greater than the sum of the anti-Her2 antibody Trastuzumab and the anti-CD47 antibody Hu5F9-G4 molecules.

(148) As known to those skilled in the art, the above test results indicate that the recombinant proteins of the present disclosure, which are capable of simultaneously binding to both the tumor-targeting antigen and CD47, can bind more to tumor cells and thus provide a more significant anti-tumor effect, compared with the concomitant use of the anti-CD47 antibody or SIRPα D1-Fc with another tumor-targeting therapeutic antibody.

(149) Determination of Bispecific Binding Activity to the Targets EGFR and CD47 by Flow Cytometry

(150) Well-grown A431 cells (human epidermal cancer cell, purchased from the Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences) were collected and counted, centrifuged and resuspended to a concentration of 2×10.sup.6 cells/mL with PBS+2% FBS. 100 μL of the cell suspension was aliquoted to each of a 96-well U-plate (Art No. 3799, Corning) and allowed to stand for at least 15 minutes. The supernatant was pipetted and discarded after centrifugation, then 11 serial dilutions of Anti-EGFR-Fc1-D1-Fc2, JMT101, SIRPα D1-Fc or Hu5F9-G4 (4-fold serial dilutions starting from 216.6 nM, a total of 11 dilutions) were added respectively and incubated at 4° C. for 1 hour. After rinsing with PBS+2% FBS, goat anti-human IgG Fc-FITC (F9512-2ML, Sigma) was added and incubated for 1 hour at 4° C. After rinsing and resuspension with PBS+2% FBS, the fluorescence value was determined by a flow cytometer (Accuri C6, BD).

(151) Since the surface of A431 cells simultaneously expresses EGFR and CD47 antigens, the anti-EGER antibody JMT101, the anti-CD47 antibody Hu5F9-G4 and SIRPα D1-Fc are all capable of specifically binding to A431 cells, but their maximum average fluorescence intensities are various.

(152) The test results showed that Anti-EGFR-Fc1-D1-Fc2, Anti-EGFR-Fc1-D1.sup.m-Fc2, Anti-EGFR-Fc1-D1-D2-Fc2, Anti-EGFR-Fc1-D1.sup.m-D2-Fc2 and Anti-EGFR-Fc1-D1-D2-D3-Fe2 were all capable of binding to A431 cells, and achieved a higher maximum average fluorescence intensity.

(153) The above test results demonstrate that the recombinant proteins of the present disclosure are capable of specifically binding to tumor cells and exhibit a significant advantage in the number of molecules under the environment of a same supersaturated protein concentration, compared with the anti-EGFR antibody JMT101 and/or the anti-CD47 antibody Hu5F9-G4 and/or SIRPα D1-Fc. Preferably, the saturation binding abundance of the recombinant proteins of the present disclosure to tumor cells is greater than the sum of the saturation binding abundance of the corresponding anti-EGFR antibody and SIRPα D1-Fc to tumor cells under the environment of a same supersaturated protein concentration.

(154) TABLE-US-00007 TABLE 6 Maximum average fluorescence intensity and EC.sub.50 (nM) of the binding of antibodies/recombinant proteins to A431 cells Sample Anti-EGFR- SIRPα JMT101 Fc1-D1-Fc2 Hu5F9-G4 D1-Fc Maximum 901868 1511274 686658 429788 average fluorescence intensity EC.sub.50 (nM) 0.598 1.217 0.865 3.677

(155) For example, as shown in FIG. 8C and Table 6, the anti-EGFR antibody JMT101, the anti-CD47 antibody Hu5F9-G4 and SIRPα D1-Fc are all capable of specifically binding to A431 cells, but their maximum average fluorescence intensities are not identical to each other; and the EC.sub.50 (JMT101)=0.598 nM, EC.sub.50 (SIRPα D1-Fc)=3.677 nM, EC.sub.50 (Hu5F9-G4)=0.865 nM. It can be seen that the binding affinity of JMT101 to A431 cells is more than 6-fold of the binding affinity of SIRPα D1-Fc to A431 cells. Meanwhile, Anti-EGFR-Fc1-D1-Fc2 is also capable of binding to A431 cells and has a higher maximum average fluorescence intensity, indicating that under the condition of a same supersaturated protein concentration, the number of Anti-EGFR-Fc1-D1-Fc2 molecules specifically binding to A431 cells is significantly greater than that of the anti-EGFR antibody JMT101 or SIRPα D1-Fcor the anti-CD47 antibody Hu5F9-G4 molecules, and also greater than the sum of the anti-EGFR antibody JMT101 and SIRPα D1-Fc molecules.

(156) As known to those skilled in the art, the above test results indicate that the recombinant proteins of the present disclosure, which are capable of simultaneously binding to both the tumor-targeting antigen and CD47, can bind more to tumor cells and thus provide a more significant anti-tumor effect, compared with the concomitant use of the anti-CD47 antibody or SIKPα D1-Pc with another tumor-targeting therapeutic antibody.

(157) Determination of Bispecific Binding Activity to the Targets PD-L1 and CD47 by Flow Cytometry

(158) 2×10.sup.7 NCI-H441 cells (human lung adenocarcinoma cell, purchased from BeinaChuanglian Biotechnology Research Institute Co., Ltd, Beijing) were stimulated with 10 ng/mL, hIFN-γ (BD, Art No. 554616), then digested, collected, counted, centrifuged and resuspended to a concentration of 3×10.sup.6 cells/mL with PBS+2% FBS. 100 μL of the cell suspension was aliquoted to each well of a 96-well U-plate (Art No. 3799, Corning) and allowed to stand for at least 15 minutes. The supernatant was pipetted and discarded after centrifugation, then 12 serial dilutions of Anti-PD-L1(Ate)-Fc1-D1-Fc2 or Atezolizumab (433.2 nM, 21.6.6 nM, 4-fold serial dilutions starting from 216.6 nM, a total of 12 concentrations) were added respectively and incubated at 4° C. for 1 hour. After rinsing with PBS+2% FBS, goat anti-human IgG Fc-FITC (F9512-2ML, Sigma) was added and incubated for 1 hour at 4° C. After rinsing and resuspension with PBS+2% FBS, the fluorescence value was determined by a flow cytometer (Accuri C6, BD).

(159) Since the surface of NCI-H441 cells simultaneously expresses PD-L1 and CD47 antigens, the anti-PD-L1 antibodies Atezolizumab, 13G4, 12A4, the anti-CD47 antibody Hu5F9-G4, SIRPαD1-Fc are all capable of specifically binding to NCI-H441cellsl.

(160) The test results showed that Anti-PD-L1(Ate)-Fc1-D1-Fc2, Anti-PD-L1(Ate)-Fc1-D1.sup.m-Fc2, Anti-PD-L1(Ate)-Fc1-D1-D2-Fc2, Anti-PD-L1(Ate)-Fc1-D1.sup.m-D2-Fc2, Anti-PD-L1(Ate)-Fc1-D1-D2-D3-Fc2, Anti-PD-L1(13G4)-Fc1-D1-Fc2, Anti-PD-L1(13G4)-Fc1-D1.sup.m-Fc2, Anti-PD-L1(13G4)-Fc1-D1-D2-Fc2, Anti-PD-L1(13G4)-Fc1-D1.sup.m-D2-Fc2, Anti-PD-L1(13G4)-Fc1-D1-D2-D3-Fc2, Anti-PD-L1(12A4)-Fc1-D1-Fc2, Anti-PD-L1(12A4)-Fc1-D1.sup.m-Fc2, Anti-PD-L1 (12A4)-Fc1-D1-D2-Fc2, Anti-PD-L1(12A4)-Fc1-D1.sup.m-D2-Fc2 and Anti-PD-L1(12A4)-Fc1-D1-D2-D3-Fc2 were all capable of binding to NCI-H441 cells, and achieved a higher maximum average fluorescence intensity.

(161) The above test results demonstrate that the recombinant proteins of the present disclosure, compared with the anti-PD-L1 antibodies Atezolizumab, 13G4 and 12A4, are capable of specifically binding to tumor cells and exhibit a significant advantage in the number of molecules under the condition of a same supersaturated protein concentration. For example, under the environment of a same supersaturated protein concentration, the recombinant proteins of the present disclosure binds more to tumor cells and exhibits a significant advantage in the number of molecules than the anti-PD-L1 antibody Atezolizumab.

(162) TABLE-US-00008 TABLE 7 Maximum average fluorescence intensity and EC.sub.50 (nM) of the binding of antibodies/recombinant proteins to NCI-H441 cells Sample Anti-PD- L1(Ate)- SIRPα Fc1-D1-Fc2 Atezolizumab Hu5F9-G4 D1-Fc Maximum 156541 64038 100973 83200 average fluorescence intensity EC50 (nM) 1.565 0.2006 0.3865 1.643

(163) For example, as shown in FIG. 8D and Table 7, the anti-PD-L1 antibody Atezolizumab is capable of specifically binding to NCI-H441 cells; meanwhile, Anti-PD-L1(Ate)-Fc1-D1-Fc2 is also capable of binding to NCI-H441 cells and has a higher maximum average fluorescence intensity, indicating that under the environment of a same supersaturated protein concentration, the number of Anti-PD-L1(Ate)-Fc1-D1-Fc2 molecules specifically binding to NCI-H441 cells is significantly greater than that of the anti-PD-L1 antibody Atezolizumab molecules. EC.sub.50 (Atezolizumab)=0.2006 nM, EC.sub.50 (SIRPα D1-Fc)=1.643 nM, EC.sub.50 (Hu5F9-G4)=0.3865 nM. It can be seen that the binding affinity of Atezolizumab to NCI-H441 cells is more than 6-fold of the binding affinity of SIRPα D1-Fc to NCI-H441 cells.

(164) As known to those skilled in the art, the above test results indicate that the recombinant proteins of the present disclosure, which are capable of simultaneously binding to both the tumor-targeting antigen and CD47, can bind more to tumor cells and thus provide a more significant anti-tumor effect, compared with the concomitant use of the anti-CD47 antibody or SIRPα D1-Fc with another tumor-targeting therapeutic antibody.

(165) 2. Determination of competitive binding activity to the target

(166) The following method takes Ofa-Fc1-D1-Fc2 or Anti-EGFR-Fc1-D1-Fc2 as an example, using ELISA to determine the competitive binding activity to the targets CD47 and SIRPα.

(167) Determination of Competitive Binding Activity of Ofa-Fc1-D1-Fc2 and Anti-EGFR-Fc1-D1-Fc2 by ELISA

(168) An ELISA plate (9018, Corning) was coated with 100 μL of 1 μg/mL CD47-4-His (12283-H08H-200, Sino Biological) and placed at 4° C. overnight. The plate was washed with PBST, and then blocked with PBS+1% BSA for 2 hours at room temperature. After rinsing, 100 μL of a mixture of diluted Ofa-Fc1-D1-Fc2 or Anti-EGFR-Fc1-D1-Fc2 (3-fold serial dilutions starting from 1000 ng/mL, a total of 11 dilutions) and biotin-labeled SIRPα D1-Fc (Biotin Labeling Kit, 21925, Thermo, the concentration for adding was 100 ng/mL) was aliquoted to each well of the coated plate, then incubated for 1 hour at 25° C. After discarding the sample and rinsing the plate three times with PBST solution, 100 μL of diluted streptavidin-HRP (1:10000) (ML-0437P-HRP, ZI501-1, Yanyu Chemical Reagent Co., Ltd) was added, then incubated at 25° C. for 1 hour. After discarding the solution and rinsing the plate three times with PBST solution, TMB (P0209, beyotime) was added, and the plate was developed for about 20 minutes and placed away from light. The reaction was stopped with H.sub.2SO.sub.4, and the OD value at 450-650 nm was read on a microplate reader.

(169) The test results showed that the anti-CD47 antibodies Hu5F9-G4, SIRPα D1-Fc, Ofa-Fc1-D1-Fc2, Ofa-Fc1-D1-D2-Fc2, Ofa-Pc1-D1-D2-D3-Fc2, Obi-Fc1-D1-Fc2, Obi-Fc1-D1-D2-Fc2, Obi-Fc1-D1-D2-D3-Fc2, Anti-EGFR-Fc1-D1-Fc2, Anti-EGFR-Fc1-D1-D2-Fc2, Anti-EGFR-Fc1-D1-D2-D3-Fc2, Anti-Her2(P)-Fc1-D1-Fc2, Anti-Her2(P)-Fc1-D1-D2-Fc2, Anti-Her2(P)-Fc1-D1-D2-D3-Fc2, Anti-Her2(T)-Fc1-D1-Fc2, Anti-Her2(T)-Fc1-D1-D2-Fc2, Anti-Her2(T)-Fc1-D1-D2-D3-Fc2, Anti-EGFR-Fc1-D1-Fc2, Anti-EGFR-Fc1-D1-D2-Fc2, Anti-EGFR-Fc1-D1-D2-D3-Fc2, Anti-PD-L1(Ate)-Fc1-D1-Fc2, Anti-PD-L1(Ate)-Fc1-D1-D2-Fc2, Anti-PD-1-1(Ate)-Fc1-D1-D2-D3-Fc2, Anti-PD-L1(13G4)-Fc1-D1-Fc2, Anti-PD-L1 (13G4)-Fc1-D1-D2-Fc2, Anti-PD-L1(13G4)-Fc1-D1-D2-D3-Fc2, Anti-PD-L1(12A4)-Fc1-D1-Fc2, Anti-PD-L1(12A4)-Fc1-D1-D2-Fc2 and Anti-PD-L1(12A4)-Fc1-D1-D2-D3-Fc2 were all capable of competing with the biotin-labeled SIRPα D1-Fc to bind to the CD47 antigen at different degrees, exerting competitive binding activity.

(170) For example, as shown in FIG. 9, the anti-CD47 antibodies Hu5F9-G4, SIRPα D1-Fc, OFa-Fc1-D1-Fc2, and Anti-EGFR-Fc1-D1-Fc2 are all capable of competing with the biotin-labeled SIRPα D1-Fc to bind to the CD47 antigen, exerting competitive binding activity; the competitive binding ability of Ofa-Fc1-D1-Fc2 or Anti-EGFR-Fc1-D1-Fc2 to CD47 is weaker than that of the anti-CD47 antibody Hu5F9-G4 or SIRPα D1-Fc, respectively, which is consistent with the results of the affinity studies described in the above examples.

EXAMPLE 4

Early Immunological Safety Evaluation of Recombinant Proteins in Vitro

(171) Early immunological safety study of the recombinant proteins Ofa-Fc1-D1.sup.m-Fc2, Ofa-Fc1-D1.sup.m-D2-Fc2, Anti-EGFR-Fc1-D1.sup.m-Fc2, Anti-EGFR-Fc1-D1.sup.m-D2-Fc2, Anti-Her2(T)-Fc1-D1.sup.m-Fc2, Anti-Her2(T)-Fc1-D1.sup.m-D2-Fc2, Anti-Her2(P)-Fc1-D1.sup.m-Fc2, Anti-Her2(P)-Fc1-D1.sup.m-D2-Fc2, Anti-PD-L1(Ate)-Fc1-D1.sup.m-Fc2, Anti-PD-L1(Ate)-Fc1-D1.sup.m-D2-Fc2, Anti-PD-L1(13G4)-Fc1-D1.sup.m-Fc2, Anti-PD-L1(13G4)-Fc1-D1.sup.m-D2-Fc2, Anti-PD-L1(12A4)-Fc1-D1.sup.m-Fc2 and Anti-PD-L1(12A4)-Fc1-D1.sup.m-D2-Fc2 were conducted respectively in vitro. Taking Ofa-Fc1-D1.sup.m-Fc2 as an example, the following method is suitable for the recombinant proteins comprising a high affinity mutant of the extracellular truncated variant of SIRPain the right arm.

(172) Early immunological safety study of the recombinant proteins Ofa-Fc1-D1-Fc2, Ofa-Fc1-D1-D2-Fc2, Ofa-Fc1-D1-D2-D3-Fc2, Obi-Fc1-D1-Fc2, Obi-Fc1-D1-D2-Fc2, Obi-Fc1-D1-D2-D3-Fc2, Anti-EGFR-Fc1-D1-Fc2, Anti-EGFR-Fc1-D1-D2-Fc2, Anti-EGFR-Fc-D1-D2-D3-Fc2, Anti-Her2(P)-Fc1-D1-Fc2, Anti-Her2(P)-Fc1-D1-D2-Fc2, Anti-Her2(P)-Fc1-D1-D2-D3-Fc2, Anti-Her2(T)-Fc1-D1-Fc2, Anti-Her2(T)-Fc1-D1-D2-Fc2, Anti-Her2(T)-Fc1-D1-D2-D3-Fc2, Anti-EGFR-Fc1-D1-Fc2, Anti-EGFR-Fc1-D1-D2-Fc2, Anti-EGFR-Fc1-D1-D2-D3-Fc2, Anti-PD-L1(Ate)-Fc1-D1-D2-Fc2, Anti-PD-L1(Ate)-Fc1-D1-D2-Fc2, Anti-PD-L1(Ate)-Fc1-D1-D2-D3-Fe2, Anti-PD-L1(13G4)-Fc1-D1-Fc2, Anti-PD-L1(13G4)-Fc1-D1-D2-Fc2, Anti-PD-L1(13G4)-Fc1-D1-D2-D3-Fc2, Anti-PD-L1(12A4)-Fc1-D1-Fc2, Anti-PD-L1 (12A4)-Fc1-D1-D2-Fc2 and Anti-PD-L1(12A4)-Fc1-D1-D2-D3-Fc2 were conducted respectively in vitro. Taking Ofa-Fc1-D1-Fc2 as an example, the following method is suitable for the recombinant proteins comprising an extracellular truncated variant of SIRPα in the tight arm.

(173) Determination of Specific Binding of Bispecific Antibodies to the Target CD47 before and after Mutation in the Right Arm by Flow Cytometry

(174) NCI-H441 cells (human lung adenocarcinoma cell, purchased from BeinaChuanglian Biotechnology Research Institute Co., Ltd, Beijing) were digested, collected, counted, centrifuged and then resuspended to a concentration of 3×10.sup.6 cells/mL with PBS+2% FBS. 100 μL of the cell suspension was aliquoted to each well of a 96-well U-plate (Art No. 3799, Corning) and allowed to stand for at least 15 minutes. The supernatant was pipetted and discarded after centrifugation, then 12 serial dilutions of Ofa-Fc1-D1-Fc2, Ofa-Fc1-D1.sup.m-Fc2, Hu5F9-G4 or Ofatutnutnab (433.2 nM, 216.6 nM, 4-fold serial dilutions starting from 216.6 nM, with a total of 12 concentrations) were added respectively and incubated at 4° C. for 1 hour. After rinsing with PBS+2% FBS, goat anti-human IgG Fe-FITC (F9512-2ML, Sigma) was added and incubated for 1 hour at 4° C. After rinsing and resuspension with PBS+2% FBS, the fluorescence value was determined by a flow cytometer (Accuri C6, BD).

(175) TABLE-US-00009 TABLE 8 Specific binding of bispecific antibodies to the target CD47 before and after mutation in the right arm by flow cytometry Sample Ofa-Fc1- Ofa-Fc1- D1-Fc2 D1m-Fc2 Hu5F9-G4 Ofatumumab Maximum 172944.90 272734.80 120133.40 3497.17 average fluorescence intensity EC.sub.50 (nM) 8.680 0.5292 0.2861 NA

(176) For example, as shown in FIG. 10 and Table 8, except that the anti-CD20 antibody Ofatumumab is not capable of binding to CD47, the anti-CD47 antibodies Hu5F9-G4, Ofa-Fc1-D1-Fc2 and Ofa-Fc1-D1.sup.m-Fc2 are all capable of binding to CD47, and the binding affinity of Ofa-Fc1-D1.sup.m-Fc2 (EC.sub.50=0.529 nM) to NCI-H441 cells is higher than that of OFa-Fc1-D1-Fc2 (EC.sub.50=8.68 nM), wherein D1.sup.m is a high affinity mutant in the D1 region of SIRPα(i.e., Seq ID No: 10 in CN106519036A).

(177) Early in Vitro Immunological Safety Evaluation Assay of Recombinant Proteins

(178) (1) Preparation of Human Effector Cell Suspension:

(179) Well-grown human NK92MI-CD16a effector cells with a stable and high expression of CD16a (purchased from Huabo Biotech Co., Ltd) were centrifuged (201 g, 5 min) to discard the supernatant, and resuspended in 5 mL MEM (free of phenol red) basal medium (purchased from Gibco, 51200-038). After counting, the cell suspension was adjusted to a cell density of 2.4×10.sup.6 cells/mL with MEM (free of phenol red) basal medium, which was used as a human effector cell suspension.

(180) (2) Incubation of Effector Cells and Antibodies:

(181) 50 μL MEM (free of phenol red) basal medium was aliquoted into each well of a 96-well clear bottom black plate, then 25 μL of every dilution of Ofa-Fc1-D1-Fc2 or Ofa-Fc1-D1.sup.m-Fc2 bispecific antibody was aliquoted into each well of the plate respectively in duplicate. 25 μL of the human effector cell suspension prepared in step (1) was added (60000 cells/well). After mixing thoroughly, the Ofa-Fc1-D1-Fc2 or Ofa-Fc1-D1.sup.m-Fc2 bispecific antibody had final serial concentration (4-fold serial dilutions starting from 433.2 nM, a total of 10 dilutions). The mixture was allowed to react at 37° C. for 5.5 hours, then lysis buffer (derived from Promega kit, G7891) was added to the control group and incubated for 0.5 hour.

(182) (3) Detection of ADCC Activity:

(183) After incubation, the uncovered plate was placed in a safety cabinet and naturally cooled to room temperature for approximately 15 minutes. 100 μL of LDH substrate reaction solution (derived from Promega kit, 67891) equilibrated at room temperature for 30 minutes was aliquoted to each well of the plate, mixed gently and then incubated for 15 minutes at room temperature. 50 μL of stop solution (derived from Promega kit, G7891) was immediately added to each well and mixed thoroughly, then the fluorescence value was determined on a microplate reader.

(184) The test results showed that the ADCC positive recombinant proteins and/or antibodies targeting CD47 resulted in mutual killing of NK cells due to the expression of CD47 antigen on NK cells. Therefore, compared to Ofa-Fc1-D1.sup.m-Fc2 or Ofa-Fc1-D1.sup.m-D2-Fc2 or Anti-EGFR-Fc1-D1.sup.m-Fc2 or Anti-EGFR-Fc1-D1.sup.m-D2-Fc2 or Anti-Her2(T)-Fc1-D1.sup.m-Fc2 or Anti-Her2(T)-Fc1-D1.sup.m-D2-Fc2 or Anti-Her2(P)-Fc1-D1.sup.m-Fc2 or Anti-Her2(P)-Fc1-D1.sup.m-D2-Fc2 or Anti-PD-L1(Ate)-Fc1-D1.sup.m-Fc2 or Anti-PD-L1(Ate)-Fc1-D1.sup.m-D2-Fc2 or Anti-PD-L1(13G4)-Fc1-D1.sup.m-Fc2 or Anti-PD-L1(13G4)-Fc1-D1.sup.m-D2-Fc2 or Anti-PD-L1(12A4)-Fc1-D1.sup.m-Fc2 or Anti-PD-L1 (12A4)-Fc1-D1.sup.m-D2-Fc2, each of which comprised a high affinity mutant of the extracellular truncated variant of SIRPα, the recombinant protein Ofa-Fc1-D1-Fc2 or Ofa-Fc1-D1-D2-Fc2 or Ofa-Fc1-D1-D2-D3-Fc2 or Obi-Fc1-D1-Fc2 or Obi-Fc1-D1-D2-Fc2 or Obi-Fc1-D1-D2-D3-Fc2 or Anti-EGFR-Fc1-D1-Fc2 or Anti-EGFR-Fc1-D1-D2-Fc2 or Anti-EGER-Fc1-D1-D2-D3-Fc2 or Anti-Her2(P)-Fc1-D1-Fc2 or Anti-Her2(P)-Fc1-D1-D2-Fc2 or Anti-Her2(P)-Fc1-D1-D2-D3-Fc2 or Anti-Her2(T)-Fc1-D1-Fc2 or Anti-Her2(T)-Fc1-D1-D2-Fc2 or Anti-Her2(T)-Fc1-D1-D2-D3-Fc2 or Anti-EGFR-Fc1-D1-Fc2 or Anti-EGFR-Fc1-D1-D2-Fc2 or Anti-EGFR-Fc1-D1-D2-D3-Fc2 or Anti-PD-L1(Ate)-Fc1-D1-Fc2 or Anti-PD-L1(Ate)-Fc1-D1-D2-Fc2 or Anti-PD-L1(Ate)-Fc1-D1-D2-D3-Fc2 or Anti-PD-L1(13G4)-Fc1-D1-Fc2 or Anti-PD-L1(13G4)-Fc1-D1-D2-Fc2 or Anti-PD-L1(13G4)-Fc1-D1-D2-D3-Fc2 or Anti-PD-L1(12A4)-Fc1-D1-Fc2 or Anti-PD-L1(12A4)-Fc1-D1-D2-Fc2 or Anti-PD-L1(12A4)-Fc1-D1-D2-D3-Fc2 significantly reduced the toxic side effects caused by NK cells for at least 1000-fold, due to its weak affinity to the CD47.

(185) For example, as shown in FIG. 11, when the concentration of the antibody/recombinant protein reached 10.sup.−1 nm, cell lysis begins to occur and when the concentration of the antibody/recombinant protein reached 10.sup.3 nM, the rate of cell lysis reaches 15.25% in the Ofa-Fc1-D1.sup.m-Fc2 treatment group, while no cell lysis is Observed in the Oba-Fc1-D1-Fc2 treatment group at a concentration of 10.sup.3 nM.

(186) As known to those skilled in the art, the above test results indicate that the recombinant proteins of the present disclosure having ADCC activity and low affinity to the CD47 antigen have higher immunological safety.

(187) As known to those skilled in the art, the above test results indicate that the optimized method for detecting ADCC activity (i.e., early immunological safety evaluation assay in vitro) described in the present disclosure can be used to evaluate the early immunological safety of recombinant proteins (including monovalent or multivalent) or antibodies (including monovalent or multivalent) targeting CD47 and having ADCC activity. The method is simple, fast and not limited by blood resources.

EXAMPLE 5

Inhibition of Tumor Cell Growth by Recombinant Protein in Vivo

(188) Inhibition of tumor cell growth by recombinant proteins Ofa-Fa-D1-Fc2, Ofa-Fc1-D1-D2-Fc2, Ofa-Fc1-D1-D2-D3-Fc2, Obi-Fc1-D1-Fc2, Obi-Fc1-D1-D2-Fc2, Obi-Fc1-D1-D2-D3-Fc2, Anti-EGFR-Fc1-D1-Fc2, Anti-EGFR-Fc1-D1-D2-Fc2, Anti-EGFR-Fc1-D1-D2-D3-Fc2, Anti-Her2(P)-Fc1-D1-Fc2, Anti-Her2(P)-Fc1-D1-D2-Fc2, Anti-Her2(P)-Fc1-D1-D2-D3-Fc2, Anti-Her2(T)-Fc1-D1-Fc2, Anti-Her2(T)-Fc1-D1-D2-Fc2, Anti-Her2(T)-Fc1-D1-D2-D3-Fc2, Anti-EGFR-Fc1-D1-Fc2, Anti-EGFR-Fc1-D1-D2-Fc2; Anti-EGFR-Fc1-D1-D2-D3-Fc2, Anti-PD-L1(Ate)-Fc1-D1-Fc2, Anti-PD-L1(Ate)-Fc1-D1-D2-Fc2, Anti-PD-L1(Ate)-Fc1-D1-D2-D3-Fc2, Anti-PD-L1(13G4)-Fc1-D1-Fc2, Anti-PD-L1(13G4)-Fc1-D1-D2-Fc2, Anti-PD-L1(13G4)-Fc1-D1-D2-D3-Fc2, Anti-PD-L1(12A4)-Fc1-D1-Fc2, Anti-PD-L1(12A4)-Fc1-D1-D2-Fc2, Anti-PD-L1(12A4)-Fc1-D1-D2-D3-Fc2 were conducted respectively in vivo. Taking Ofa-Fc1-D1-Fc2 as an example, the following method is applicable for the detection of recombinant proteins comprising an extracellular truncated variant of SIRPα in the right arm.

(189) Male NSG mice (purchased from Beijing Wino Co., Ltd) were subcutaneously inoculated with human B-cell lymphoma Raji cells. After the tumor volume reached 80 mm.sup.3 to 150 mm.sup.3, the mice were divided into the following 2 groups (6 mice per group, the mice were intraperitoneally injected with the given agents for each group): 1) vehicle control group (Tris-citrate, pH 6.5); 2) Ofa-Fc1-D1-Fc2 group (150 μg/mouse); twice a week for 2 weeks. Tumor growth was observed and the tumor volume was measured before administration (0 day), and on the 3.sup.rd day, 5.sup.th day, 7.sup.th day, 10.sup.th day, 12.sup.th day, and 14.sup.th day after administration, to evaluate the anti-tumor effect of Ofa-Fc1-D1-Fc2.

(190) The test results showed that in the NSG mouse model subcutaneously transplanted with Raji lymphoma, the recombinant proteins Ofa-Fc1-D1-Fc2, Ofa-Fc1-D1-D2-Fc2, Ofa-Fc1-D1-D2-D3-Fc2, Obi-Fc1-D1-Fc2, Obi-Fc1-D1-D2-Fc2, Obi-Fc1-D1-D2-D3-Fc2, Anti-EGFR-Fc1-D1-Fc2, Anti-EGFR-Fc1-D1-D2-Fc2, Anti-EGFR-Fc1-D1-D2-D3-Fc2, Anti-Her2(P)-Fc1-D1-Fc2, Anti-Her2(P)-Fc1-D1-D2-Fc2, Anti-Her2(P)-Fc1-D1-D2-D3-Fc2, Anti-Her2(T)-Fc1-D1-Fc2, Anti-Her2(T)-Fc1-D1-D2-Fc2, Anti-Her2(T)-Fc1-D1-D2-D3-Fc2, Anti-EGFR-Fc1-D1-Fc2, Anti-EGFR-Fc1-D1-D2-Fc2, Anti-EGFR-Fc1-D1-D2-D3-Fc2, Anti-PD-L1(Ate)-Fc1-D1-Fc2, Anti-PD-L1(Ate)-Fc1-D1-D2-Fc2, Anti-PD-L1(Ate)-Fc1-D1-D2-D3-Fc2, Anti-PD-L1(13G4)-Fc1-D1-Fc2, Anti-PD-L1(13G4)-Fc1-D1-D2-Fc2, Anti-PD-L1 (13G4)-Fc1-D1-D2-D3-Fc2, Anti-PD-L1(12A4)-Fc1-D1-Fc2, Anti-PD-L1(12A4)-Fc1-D1-D2-Fc2 and Anti-PD-L1(12A4)-Fc1-D1-D2-D3-Fc2 blocked the CD47-SIRPα signaling pathway and thus activated targeted phagocytosis of macrophages and/or Antibody dependent cellular phagocytosis (ADCP) mediated by macrophages, thus exhibiting a significant tumor suppressive effect.

(191) For example, as shown in FIG. 12, the abscissa represents the time (days) from the NSG mice subcutaneously transplanted with Raji lymphoma receive drug treatment, and the ordinate represents the tumor volume (mm.sup.3). FIG. 12 shows that after a period of treatment with the drug Ofa-Fc1-D1-Fc2, the Ofa-Fc1-D1-Fc2 group shows a significant tumor suppressive trend compared to the vehicle control group, and the tumor inhibition rate of the Ofa-Fc1-D1-Fc2 group on the 14.sup.th day reaches 63.14%.

(192) As known to those skilled in the art, the above test results indicate that the recombinant proteins of the present disclosure which are capable of simultaneously binding to both the target antigen and the CD47 antigen on the tumor cells can achieve a significant tumor suppressing effect in the NSG mice subcutaneously transplanted with tumor cells.

EXAMPLE 6

Early Immunological Safety Evaluation Assay of Recombinant Proteins in Vitro

(193) Early immunological safety evaluation of recombinant proteins Ofa-Fc1-D1.sup.m-Fc2, Ofa-Fc1-D1.sup.m-D2-Fc2, Anti-EGFR-Fc1-D1.sup.m-Fc2, Anti-EGFR-Fc1-D1.sup.m-D2-Fc2, Anti-Her2(T)-Fc1-D1.sup.m-Fc2, Anti-Her2(T)-Fc1-D1.sup.m-D2-Fc2, Anti-Her2(P)-Fc1-D1.sup.m-Fc2, Anti-Her2(P)-Fc1-D1.sup.m-D2-Fc2, Anti-PD-L1(Ate)-Fc1-D1.sup.m-Fc2, Anti-PD-L1(Ate)-Fc1-D1.sup.m-D2-Fc2, Anti-PD-L1(13G4)-Fc1-D1.sup.m-Fc2, Anti-PD-L1(13G4)-Fc1-D1.sup.m-D2-Fc2, Anti-PD-L1(12A4)-Fc1-D1.sup.m-Fc2 and Anti-PD-L1(12A4)-Fc1-D1.sup.m-D2-Fc2 were conducted respectively in vitro. Taking Ofa-Fc1-D1.sup.m-Fc2 as an example, the following method is applicable for the detection of recombinant proteins comprising a high affinity mutant of the extracellular truncated variant of SIRPct in the right arm.

(194) Early immunological safety evaluation of recombinant proteins Ofa-Fc1-D1-Fc2, Ofa-Fc1-D1-D2-Fc2, Ofa-Fc1-D1-D2-D3-Fc2, Obi-Fc1-D1-D2-Fc2, Obi-Fc1-D1-D2-D3-Fc2, Anti-EGFR-Fc1-D1-Fc2, Anti-EGFR-Fc1-D1-D2-Fc2, Anti-EGFR-Fc1-D1-D2-D3-Fc2, Anti-Her2(P)-Fc1-D1-Fc2, Anti-Her2(P)-Fc1-D1-D2-Fc2, Anti-Her2(P)-Fc1-D1-D2-D3-Fc2, Anti-Her2(T)-Fc1-D1-Fc2, Anti-Her2(T)-Fc1-D1-D2-Fc2, Anti-Her2(T)-Fc1-D1-D2-D3-F2, Anti-EGFR-Fc1-D1-Fc2, Anti-EGER-Fc1-D1-D2-Fc2, Anti-EGFR-Fc1-D1-D2-D3-Fc2, Anti-PD-L1(Ate)-Fc1-D1-Fc2, Anti-PD-L1(Ate)-Fc1-D1-D2-Fc2, Anti-PD-L1(Ate)-Fc1-D1-D2-D3-Fc2, Anti-PD-L1(13G4)-Fc1-D1-Fc2, Anti-PD-L1(13G4)-Fc1-D1-D2-Fc2, Anti-PD-L1(13G4)-Fc1-D1-D2-D3-Fc2, Anti-PD-L1(12A4)-Fc1-D1-Fc2, Anti-PD-L1(12A4)-Fc1-D1-D2-Fe2 and Anti-PD-L1(12A4)-Fc1-D1-D2-D3-Fc2 were conducted respectively in vitro. Taking Ofa-Fc1-D1-Fc2 as an example, the following method is applicable for detection of recombinant proteins comprising an extracellular truncated variant of SIRPα in the right arm.

(195) Since B cells highly express the CD20 antigen, this experiment evaluates the killing of tumor cells by determining the B cell content. In early immunological safety evaluation assay of recombinant proteins targeting another tumor antigen and the CD47 antigen in vitro, the experimental mice in this example should be subcutaneously transplanted with corresponding tumor cells.

(196) Specific Tumor-Targeting Effect:

(197) NSG (Hu-NSG) female mice (purchased from Beijing Idmo Co., Ltd) transplanted with human CD34.sup.+ HSC were selected and divided into the following 3 groups (3 mice for each group, the mice were intravenously injected with the given agents for each group): 1) 0.9% saline control group; 2) Hu5F9-G4 group (6.7 μg/mouse); 3) Ofa-Fc1-D1-Fc2 group (5 μg/mouse). The mice were administered once. At 96 hours after administration, 80 μL of blood was collected from the tail vein of the mice and added to an anticoagulant tube containing heparin sodium. The RBCs were lysed by a freshly prepared mixture of lysis buffer (BD Pharm Lyse™, Art No. 555899) and double distilled water in a volume ratio of 1:1, and the remaining cells were rinsed and resuspended with PBS+2% FBS, and then incubated with the fluorescent antibody (PE anti-human CD45 (Art No. 304039), FITC anti-human CD19 (Art No. 302206), APC anti-human CD3 (Art No. 300312), all purchased from BioLegend) for 30 minutes. After rinsing and resuspension with PBS+2% FBS, the sample was detected by a flow cytometer (Accuri™ C6, BD).

(198) The test results showed that, when Hu-NSG mice were administrated with a same dose of Ofa-Fc1-D1-Fc2 or the anti-CD47 antibody Hu5F9-G4, at 96 hours after administration, Ofa-Fc1-D1-Fc2 preferentially cleared B cells expressing CD20 antigen (i.e., target cells), while the anti-CD47 antibody Hu5F9-G4 preferentially cleared non-target cells with high abundance of CD47 expression (such as T cells), due to its high affinity to the CD47 antigen.

(199) For example, as shown in FIG. 13, the anti-CD47 antibody Hu5F9-G4 group (FIG. 13B) shows a significant clearance of non-target cells with high abundance of CD47 expression (such as T cells) compared to 0.9% normal saline group (FIG. 13A) (the proportion of B cells (i.e., the target cells with CD20 antigen) in all detected cells increases from 40.9% to 73.5% at 96 hours after administration); whereas the recombinant protein Ofa-Fc1-D1-Fc2 group (FIG. 13C) shows a significant clearance of B cells (i.e., the target cells with the CD20 antigen) compared with 0.9% normal saline (FIG. 13A), i.e., Ofa-Fc1-D1-Fc2 preferentially clears B cells at 96 hours after administration (the proportion of B cells in all detected cells decreases from 40.9% to 3.7% at 96 hours after administration).

(200) As known to those skilled in the art, the above test results indicate that the recombinant proteins of the present disclosure which are capable of simultaneously binding to both the tumor-targeting antigen and the CD47 antigen preferentially clear the cells and/or tumor cells with the tumor-targeting antigen under the condition of a same dose.

(201) Immunological Safety at a Low Dose:

(202) NSG (Hu-NSG) female mice (purchased from Beijing Idmo Co., Ltd) transplanted with human CD34+ HSC were selected and divided into the following 2 groups (3 mice for each group, the mice were intravenously injected with the given agents for each group): 1) Ofa-Fc1-D1-Fc2 group (1 μg/mouse); 3) Ofa-Fc1-D1.sup.m-Fc2 group (1 μg/mouse). The mice were administered once. At 72 hours after administration, 80 μL of blood was collected from the tail vein of the mice and added to an anticoagulant tube containing heparin sodium. The RBC's were lysed by a freshly prepared mixture of lysis buffer (BD Pharm Lyse™, Art No. 555899) and double distilled water at a volume ratio of 1:1, and the remaining cells were rinsed and resuspended with PBS+2% FBS, and then incubated with the fluorescent antibody (PE anti-human CD45 (Art No. 304039), FITC anti-human CD19 (Art No. 302206), APC anti-human CD3 (Art No. 300312), all purchased from BioLegend) for 30 minutes. After rinsing and resuspension with PBS+2% FBS, the sample of the Ofa-Fc1-D1-Fc2 group (1 μg/mouse) was detected by a flow cytometer (Accuri™ C6, BD), and the sample of the Ofa-Fc1-D1.sup.m-Fc2 group (1 μg/mouse) was detected by a flow cytometer (NovoCyte™ 3130, ACEA).

(203) The test results showed that, in the case where the Hu-NSG mice were administrated with recombinant proteins at a low dose, at 72 hours after Ofa-Fc1-D1-Fc2, Ofa-Fc1-D1-D2-Fc2, Ofa-Fc1-D1-D2-D3-Fc2, Obi-Fc1-D1-Fc2, Obi-Fc1-D1-D2-Fc2, Obi-Fc1-D1-D2-D3-Fc2, Anti-EGFR-Fc1-D1-Fc2, Anti-EGFR-Fc1-D1-D2-Fa, Anti-EGFR-Fc1-D1-D2-D3-Fc2, Anti-Her2(P)-Fc1-D1-Fc2, Anti-Her2(P)-Fc1-D1-D2-Fc2, Anti-Her2(P)-Fc1-D1-D2-D3-Fc2, Anti-Her2(T)-Fc1-D1-Fc2, Anti-Her2(T)-Fc1-D1-D2-Fc2, Anti-Her2(T)-Fc1-D1-D2-D3-Fc2, Anti-EGFR-Fc1-D1-Fc2, Anti-EGFR-Fc1-D1-D2-Fc2, Anti-EGFR-Fc1-D1-D2-D3-Fc2, Anti-PD-L1(Ate)-Fc1-D1-Fc2, Anti-PD-L1(Ate)-Fc1-D1-D2-Fc2, Anti-PD-L1(Ate)-Fc1-D1-D2-D3-Fc2, Anti-PD-L1(13G4)-Fc1-D1-Fc2, Anti-PD-L1(13G4)-Fc1-D1-D2-Fc2, Anti-PD-L1 (13G4)-Fc1-D1-D2-D3-Fc2, Anti-PD-L1(12A4)-Fc1-D1-Fc2, Anti-PD-L1(12A4)-Fc1-D1-D2-Fc2 or Anti-PD-L1(12A4)-Fc1-D1-D2-D3-Fc2 was administrated once, the target cells which expressed the antigen targeted by the left arm (such as tumor cells) had been significantly eliminated, whereas the cells which do not express the antigen targeted by the left arm (such as T cells, other immune cells) had not been significantly affected; however, at 72 hours after the recombinant protein comprising a high affinity mutant of the extracellular truncated variant of SIRPα, Ofa-Fc1-D1.sup.m-D2-Fc2 or Anti-EGFR-Fc1-D1.sup.m-Fc2 or Anti-EGFR-Fc1-D1.sup.m-D2-Fc2 or Anti-Her2(T)-Fc1-D1.sup.m-Fc2 or Anti-Her2(T)-Fc1-D1.sup.m-D2-Fc2 or Anti-Her2(P)-Fc1-D1.sup.m-Fc2 or Anti-Her2(P)-Fc1-D1.sup.m-D2-Fc2 or Anti-PD-L1(Ate)-Fc1-D1.sup.m-Fc2 or Anti-PD-L1(Ate)-Fc1-D1.sup.m-D2-Fc2 or Anti-PD-L1(13G4)-Fc1-D1.sup.m-Fc2 or Anti-PD-L1(13G4)-Fc1-D1.sup.m-D2-Fc2 or Anti-PD-L1(12A4)-Fc1-D1.sup.m-Fc2 or Anti-PD-L1(12A4)-Fc1-D1.sup.m-D2-Fc2 was administrated once, although the target cells which expressed the antigen targeted by the left arm (such as tumor cells) had been significantly eliminated, the cells which do not express the antigen targeted by the left arm (such as T cells and other immune cells) also had been eliminated to a significant extent.

(204) For example, as shown in FIG. 14, at 72 hours after the single administration of Ofa-Fc1-D1-Fc2B (FIG. 14C, FIG. 14D), the B cells (the target cell with the CD20 antigen) have been significantly eliminated (almost completely eliminated), while the cells that do not express the CD20 antigen (such as cells, other immune cells) have not been significantly affected, compared to the state before administration (FIG. 14A, FIG. 14B). At 72 hours after the single administration of Ofa-Fc1-D1.sup.m-Fc2 (FIG. 14G, FIG. 14H), compared to the state before administration (FIG. 14E, FIG. 14F), although the B cells (the target cell with the CD20 antigen) have been significantly eliminated, the cells that do not express the CD20 antigen (such as T cells and other immune cells) also have been eliminated to a significant extent.

(205) As known to those skilled in the art, the above results indicate that the recombinant proteins comprising an extracellular truncated variant of SIRPα in the right arm have a higher specific tumor-targeting effect and exhibit a greater immunological safety than the recombinant proteins comprising a high affinity mutant of the extracellular truncated variant of SIRPα in the right arm at a same dose.

(206) Immune Recovery at a High Dose:

(207) NSG (Hu-NSG) female mice (purchased from Beijing Idmo Co., Ltd) transplanted with human CD34.sup.+ HSC were selected and divided into the following 2 groups (3 mice for each group, the mice were intravenously injected with the given agents for each group): 1) Hu5F9-G4 group (200 μg/mouse), 3) Ofa-Fc1-D1-Fc2 group (150 μg/mouse). The mice were administered once. At 4 days and 14 days after administration, 80 μL of blood was collected from the tail vein of the mice and added to an anticoagulant tube containing heparin sodium. The RBCs were lysed by a freshly prepared mixture of lysis buffer (BD Pharm Lyse™, Art No. 555899) and double distilled water in a volume ratio of 1:1, and the remaining cells were washed and resuspended with PBS+2% FBS, and then incubated with the fluorescent antibody (PE anti-human CD45 (Art No. 304039), FITC anti-human CD19 (Art No. 302206), APC anti-human CD3 (Art No. 300312), all purchased from BioLegend) for 30 minutes. After rinsing and resuspension with PBS+2% FBS, the sample was detected by a flow cytometer (Accuri™ C6, BD).

(208) The test results showed that, in the case where the Hu-NSG mice were administrated with recombinant proteins at a high dose, at 96 hours after Ofa-Fc1-D1-Fc2, Ofa-Fc1-D1-D2-Fc2, Ofa-Fc1-D1-D2-D3-Fc2, Obi-Fc1-D1-Fc2, Obi-Fc1-D1-D2-Fc2, Obi-Fc1-D1-D2-D3-Fc2, Anti-EGFR-Fc1-D1-Fc2, Anti-EGFR-Fc1-D1-D2-Fc2, Anti-EGFR-Fc1-D1-D2-D3-Fc2, Anti-Her2(P)-Fc1-D1-Fc2, Anti-Her2(P)-Fc1-D1-D2-Fc2, Anti-Her2(P)-Fc1-D1-D2-D3-Fc2, Anti-Her2(T)-Fc1-D1-Fc2, Anti-Her2(T)-Fc1-D1-D2-Fc2, Anti-Her2(T)-Fc1-D1-D2-D3-Fc2, Anti-EGFR-Fc1-D1-Fc2, Anti-EGFR-Fc1-D1-D2-Fc2, Anti-EGFR-Fc1-D1-D2-D3-Fc2, Anti-PD-L1(Ate)-Fc1-D1-Fc2, Anti-PD-L1(Ate)-Fc1-D1-D2-Fc2, Anti-PD-L1(Ate)-Fc1-D1-D2-D3-Fc2, Anti-PD-L1(13G4)-Fc1-D1-Fc2, Anti-PD-L1(13G4)-Fc1-D1-D2-Fc2, Anti-PD-L1(13G4)-Fc1-D1-D2-D3-Fc2, Anti-PD-L1(12A4)-Fc1-D1-Fc2, Anti-PD-L1(12A4)-Fc1-D1-D2-Fc2, Anti-PD-L1(12A4)-Fc1-D1-D2-D3-Fc2 or anti-CD47 antibody Hu5F9-G4 was administrated once, the B cells (the target cell with the CD20 antigen) and the non-target cells (such as T cells, other immune cells) had been eliminated to a significant extent in each group. At 14 days after administration, in the Ofa-Fc1-D1-Fc2, Ofa-Fc1-D1-D2-Fc2, Ofa-Fc1-D1-D2-D3-Fc2, Obi-Fc1-D1-Fc2, Obi-Fc1-D1-D2-Fc2, Obi-Fc1-D1-D2-D3-Fc2, Anti-EGFR-Fc1-D1-Fc2, Anti-EGFR-Fc1-D1-D2-Fc2, Anti-EGFR-Fc1-D1-D2-D3-Fc2, Anti-Her2(P)-Fc1-D1-Fc2, Anti-Her2(P)-Fc1-D1-D2-Fc2, Anti-Her2(P)-Fc1-D1-D2-D3-Fc2, Anti-Her2(T)-Fc1-D1-Fc2, Anti-Her2(T)-Fc1-D1-D2-Fc2, Anti-Her2(T)-Fc1-D1-D2-D3-Fc2, Anti-EGFR-Fc1-D1-Fc2, Anti-EGFR-Fc1-D1-D2-Fc2, Anti-EGFR-Fc1-D1-D2-D3-Fc2, Anti-PD-L1(Ate)-Fc1-D1-Fc2, Anti-PD-L1(Ate)-Fc1-D1-D2-Fc2, Anti-PD-L1(Ate)-Fc1-D1-D2-D3-Fc2, Anti-PD-L1(13G4)-Fc1-D1-Fc2, Anti-PD-L1(13G4)-Fc1-D1-D2-Fc2, Anti-PD-L1(13G4)-Fc1-D1-D2-D3-Fc2, Anti-PD-L1(12A4)-Fc1-D1-Fc2, Anti-PD-L1(12A4)-Fc1-D1-D2-Fc2 or Anti-PD-L1(12A4)-Fc1-D1-D2-D3-Fc2 group, the B cells (the target cell with the CD20 antigen) were still in a state of being eliminated, while other non-target cells expressing the CD47 (such as T cells) had been significantly recovered; however in the anti-CD47 antibody Hu5F9-G4 group, neither the B cells (the target cell with the CD20 antigen) nor the non-target cells expressing the CD47 had been recovered.

(209) For example, as shown in FIG. 15, at 96 hours after administration of a high dose of the anti-CD47 antibody Hu5F9-G4 (FIG. 15A) or Ofa-Fc1-D1-Fc2 (FIG. 15B), both the B cells (the target cell with the CD20 antigen) and the non-target cells expressing the CD47 (such as T cells) have been largely eliminated; but at 14 days after administration, the B cells (the target cell with the CD20 antigen) are still in a state of being eliminated, while the non-target cells expressing the CD47 (such as T cells) except of the B cells (the target cell with the CD20 antigen) have been significantly recovered in the Ofa-Fc1-D1-Fc2 group (FIG. 15D); however, neither the B cells (the target cell with the CD20 antigen) nor the non-target cells expressing the CD47 (such as T cells) do show a sign of recovery in the anti-CD47 antibody Hu5F9-G4 group (FIG. 15C).

(210) As known to those skilled in the art, the above test results indicate that the recombinant proteins of the present disclosure which are capable of simultaneously binding to both the tumor-targeting antigen and the CD47 antigen have a higher immunological safety, since the non-target cells expressing CD47 (such as immune cells such as T cells) can be recovered under the treatment with a high dose of these recombinant proteins.

(211) As known to those skilled in the art, the above test results indicate that the early immunological safety evaluation method in vitro described in the present disclosure can be used to evaluate the early immunological safety of recombinant proteins (including monovalent or multivalent) or antibodies (including monovalent or multivalent).

EXAMPLE 7

Influence of Different Truncations on the Binding Affinity to the Target

(212) Among the recombinant proteins Ofa-Fc1-D1-Fc2, Ofa-Fc1-D1-D2-Fc2, Ofa-Fc1-D1-D2-D3-Fc2, Obi-Fc1-D1-Fc2, Obi-Fc1-D1-D2-Fc2, Obi-Fc1-D1-D2-D3-Fc2, Anti-EGFR-Fc1-D1-Fc2, Anti-EGFR-Fc1-D1-D2-Fc2, Anti-EGFR-Fc1-D1-D2-D3-Fc2, Anti-Her2(P)-Fc1-D1-Fc2, Anti-Her2(P)-Fc1-D1-D2-Fc2, Anti-Her2(P)-Fc1-D1-D2-D3-Fc2, Anti-Her2(T)-Fc1-D1-Fc2, Anti-Her2(T)-Fc1-D1-D2-Fc2, Anti-Her2(T)-Fc1-D1-D2-D3-Fc2, Anti-EGFR-Fc1-D1-Fc2, Anti-EGFR-Fc1-D1-D2-Fc2, Anti-EGFR-Fc1-D1-D2-D3-Fc2, Anti-PD-L1(Ate)-Fc1-D1-Fc2, Anti-PD-L1(Ate)-Fc1-D1-D2-Fc2, Anti-PD-L1(Ate)-Fc1-D1-D2-D3-Fc2, Anti-PD-L1(13G4)-Fc1-D1-Fc2, Anti-PD-L1(13G4)-Fc1-D1-D2-Fc2, Anti-PD-L1(13G4)-Fc1-D1-D2-D3-Fc2, Anti-PD-L1(12A4)-Fc1-D1-Fc2, Anti-PD-L1(12A4)-Fc1-D1-D2-Fc2 and Anti-PD-L1(12A4)-Fc1-D1-D2-D3-Fc2, Anti-Her2(T)-Fc1-D1-Fc2, Anti-Her2(T)-Fc1-D1-D2-Fc2, Anti-Her2(P)-Fc1-D1-Fc2 and Anti-Her2(P)-Fc1-D1-D2-Fc2 were taken as examples, and the following method is applicable for the recombinant proteins having a same left arm and a different length of extracellular truncated variants of SIRPα.

(213) Determination of Bispecific Binding Activity to the Targets Her2 and CD47 by Flow Cytometry

(214) Well-grown SKBR-3 cells (human breast cancer cell, purchased from the Cell Bank of Chinese Academy of Sciences, Shanghai) were collected, counted, centrifuged and resuspended to a concentration of 2×10.sup.6 cells/mL with PBS+2% FBS. 100 μL of the cell suspension was aliquoted to each well of a 96-well U-plate (Art No. 3799, Corning) and allowed to stand for at least 15 minutes. The supernatant was pipetted and discarded after centrifugation, then 11 serial dilutions of Anti-Her2(T)-Fc1-D1-Fc2, Anti-Her2(T)-Fc1-D1-D2-Fc2, Anti-Her2(P)-Fc1-D1-Fc2 or Anti-Her2(P)-Fc1-D1-D2-Fc2 (4-fold serial dilutions starting from 433.2 nM, a total of 11 dilutions) were added respectively and incubated at 4° C. for 1 hour. After rinsing with PBS+2% FBS, goat anti-human IgG Fc-FITC (F9512-2ML, Sigma) was added and incubated for 1 hour at 4° C. After rinsing and resuspension with PBS+2% FBS, the fluorescence value was determined by a flow cytometer (Accuri C6, BD).

(215) Since Trastuzumab and Pertuzumab act on different epitopes of the Her2 antigen and there is a large difference in the distances from the two epitopes to the cell membrane, therefore, the recombinant proteins Anti-Her2(T)-Fc1-D1-Fc2, Anti-Her2(T)-Fc1-D1-D2-Fc2, Anti-Her2(P)-Fc1-D1-Fc2 and Anti-Her2(P)-Fc1-D1-D2-Fc2 are all capable of specifically binding to SKBR-3 cells, but their binding affinities and their maximum average fluorescence intensities are various.

(216) The test results showed that, since Trastuzumab and Her2 epitopes (Pedersen M W, et al. Targeting Three Distinct HER2 Domains with a Recombinant Antibody Mixture Overcomes Trastuzumab Resistance. Molecular Cancer Therapeutics, 2015, 14(3): 669-680) are relatively closer to the cell membrane surface, Anti-Her2(T)-Fc1-D1-Fc2 had better affinity to the SKBR-3 cells than Anti-Her2(T)-Fc1-D1-D2-Fc2. Since Pertuzumab and Her2 epi topes (extracellular domain II of Her2) are relatively farther to the cell membrane surface, therefore Anti-Her2(P)-Fc1-D1-Fc2 had an equivalent affinity to the SKBR-3 cells compared to Anti-Her2(P)-Fc1-D1-D2-Fc2 SKBR-3.

(217) The above test results demonstrate that the right arm with different lengths of truncation will affect the affinity of the recombinant proteins to the target cells. For the left arm which binds to the membrane-proximal epitope, the right arm with a shorter truncation of SIRPα can largely enhance the binding of the recombinant proteins to the two targets. However, for the left arm which binds to the membrane-distal epitope, the right arm with a shorter truncation of SIRPα would lose its advantage. The farther the antigen targeted by the left arm is distant from the cell membrane, the longer the truncated variant of SIRPα in the right arm should be in order to achieve optimal matching.

(218) For example, as shown in FIG. 16, the binding capability of Anti-Her2(T)-Fc1-D1-Fc2 (EC.sub.50=2.04 nM) to SKBR-3 cells is significantly better than that of Anti-Her2(T)-Fc1-D1-D2-Fc2 (EC.sub.50=25.95 nM) (FIG. 16A). The binding capability of Anti-Her2(P)-Fc1-D1-Fc2 to SKBR-3 cells (EC.sub.50=15.22 nM) is equivalent to that of Anti-Her2(P)-Fc1-D1-D2-Fc2 (EC.sub.50=11.03 nM) (FIG. 16B).

(219) As known to those skilled in the art, the above test results indicate that based on the distance between the epitope of the target antigen and the membrane surface of the target cell, an extracellular truncated variant of human SIRPα with a suitable length for the right arm can effectively enhance the binding capability of the recombinant proteins to the target cell.

EXAMPLE 8

Acute Cytotoxicity Test of the Recombinant Proteins

(220) This embodiment provides an acute cytotoxicity test of the recombinant proteins Ofa-Fc1-D1-Fc2, Ofa-Fc1-D1-D2-Fc2, Ofa-Fc1-D1-D2-D3-Fc2, Obi-Fc1-D1-Fc2, Obi-Fc1-D1-D2-Fc2, Obi-Fc1-D1-D2-D3-Fc2, Anti-EGFR-Fc1-D1-Fc2, Anti-EGFR-Fc1-D1-D2-Fc2, Anti-EGFR-Fc1-D1-D2-D3-Fc2, Anti-Her2(P)-Fc1-D1-Fc2, Anti-Her2(P)-Fc1-D1-D2-Fc2, Anti-Her2(P)-Fc1-D1-D2-D3-Fc2, Anti-Her2(P)-Fc1-D1-Fc2, Anti-Her2(T)-Fc1-D1-D2-Fc2, Anti-Her2(T)-Fc1-D1-D2-D3-Fc2, Anti-EGFR-Fc1-D1-Fc2, Anti-EGFR-Fc1-D1-D2-Fc2, Anti-EGER-Fc1-D1-D2-D3-Fc2, Anti-PD-L1(Ate)-Fc1-D1-Fc2, Anti-PD-L1(Ate)-Fc1-D1-D2-Fc2, Anti-PD-L1 (Ate)-Fc1-D1-D2-D3-Fc2, Anti-PD-L1(13G4)-Fc1-D1-Fc2, Anti-PD-L1 (13G4)-Fc1-D1-D2-Fc2, Anti-PD-L1(13G4)-Fc1-D1-D2-D3-Fc2, Anti-PD-L1(12A4)-Fc1-D1-Fc2, Anti-PD-L1(12A4)-Fc1-D1-D2-Fc2 and Anti-PD-L1(12A4)-Fc1-D1-D2-D3-Fc2. The following method takes Ofa-Fc1-D1-Fc2 as an example, which is also applicable for the recombinant proteins with an extracellular truncated variant of SIRPα in the right arm.

(221) A proper amount of Ofa-Fc1-D1-Fc2 solution (4.02 mg/mL) was diluted to 0.25 Inglint, and 2.5 mg/mL, with a buffer (Tris-citrate, pH 6.5) for injection, and used for administrating to the group 1 and group 2 experimental animals, respectively.

(222) Four healthy female cynomolgus monkeys, aged 4, were purchased from Guangxi Guidong Primates Development and Experiment Co., Ltd. The production of the experimental animals is approved by the Department of Science and Technology of the Guangxi Zhuang Autonomous Region, and the license number is SCAK Gui2016-0001. The experimental animals were clinically observed in detail and weighed before administration, and no abnormalities were found. The body weight was 2.47-2.85 kg on the day of initial administration.

(223) TABLE-US-00010 TABLE 9 Experimental design Dosing Dosing Dose* Concentration volume Animal No. Group Treatment (mg/kg) (mg/mL) (mg/kg) Male Female 1 Ofa-Fc1- 0.5 0.25 2 0 2 D1-Fc2 2 Ofa-Fc1- 5 2.5 2 0 2 D1-Fc2 Note: *intravenous injection.

(224) Four female cynomolgus monkeys were divided into two groups with two monkeys in each group. The animals were intravenously administered at a dose of 0.5 mg/kg and 5 mg/kg respectively, and the dosing volume was 2 mL/kg. The experimental design was shown in Table 9. The animals were administrated once, and then continuously observed for 28 days after the administration. The cynomolgus monkeys were housed in stainless steel movable cages with one animal in each cage. The light was approximately 12 hours on and 12 hours off daily. The feed for the animals were purchased from Beijing KeaoXieli Feed Co., Ltd., and the animals had free access to the feed during the experiment, except for specific fasting period. The batch of the feed was detected by Shanghai Pony Testing Technology Co., Ltd. (PONY) for specific microorganisms, heavy metals and pesticide residues. During the experiment, all animals had free access to drink water via water bottles. The drinking water was purified water filtered and sterilized by reverse osmosis system. The pH, hardness, heavy metals and microorganisms of the drinking water were detected by a gauger.

(225) All animals were observed twice a day (once in the morning and once in the afternoon) near the cage during the experiment, and observations included but were not limited to morbidity, damage, death and the supply of feed and water. All animals were clinically observed in detail once before the experiment. All animals were clinically observed in detail at least once a day after administration during the experiment. Clinical observations included but were not limited to, morbidity, mortality, damage and the supply of feed and water, skin, hair, eyes, ears, nose, mouth, chest, abdomen, external genitalia, limbs, respiratory and circulatory systems, autonomic effects (such as salivation) nervous system (such as fremitus, convulsion, stress reaction, and abnormal behavior). The body weight of the animals was measured on D-1 (before administration), D1, D4, D8, D11, D15, D18, D22, D25 and before dissection. The food consumption of the animals within 24 h (24 h±1 h) was measured on D2, D4, D8, D11, D15, D18, D22, D25, respectively. Electrocardiogram was monitored on D-1, D2, D14, and D28 using a standard II lead (8 leads) at a recording speed of 50 mm/second.

(226) Clinicopathological samples were collected and hematology, blood coagulation, blood biochemistry index and lymphocyte typing were detected before administration (D-1) and on D2, D7, D14 and D28 after administration. Urine samples were collected and analyzed before administration and on day 28 after administration.

(227) Before collecting the sample, all animals were fasted except for free access to water overnight (at least 10 hours). Blood samples (4.5-6 mL) were collected from the femoral vein, wherein approximately 1.8 mL of the whole blood was used for blood coagulation analysis in an anticoagulant tube containing sodium citrate; approximately 1 mL of the whole blood was used for hematology analysis in an anticoagulant tube containing K3-EDTA; approximately 2 mL of the whole blood was used for blood biochemistry analysis in a blood collection tube (free of anticoagulant) with separator gel, and the serum was isolated by centrifugation according to standard operating procedures. Meanwhile, the isolated serum samples on D-1 before administration and on D2 after administration were used for T/B cell typing by flow cytometry.

(228) Animals are euthanized on D29, heart, liver, spleen, lung and kidney tissues were collected and preserved, and liver, lung (including main bronchus), kidney, spleen, heart, adrenal gland, pituitary, thyroid and parathyroid gland, thymus, ovary, uterus (including the cervix), and brain were weighed.

(229) The results showed that, after a single intravenous administration of Ofa-Fc1-D1-Fc2 at a dose of 0.5 mg/kg and 5 mg/kg respectively, the animals were continuously observed for 28 days after administration, no significant abnormalities related to the drug were observed in the animals, the food consumption and body weight were all fluctuated within the normal range; compared to the data before administration, the blood coagulation, urine and electrocardiogram data of the animals after administration had no significant changes; after the animals were dissected, all organs were observed to be within the normal range, and the weight of the organs, visceral coefficient, and the visceral-brain ratio were also within the normal range.

(230) On D2 after administration, the lymphocyte count and the proportion of lymphocytes in the low and high dose groups showed a significant decrease, and returned to a normal level after D7. This change may be related to the effect of the drug.

(231) The recombinant proteins Ofa-Fc1-D1-D2-Fc2, Ofa-Fc1-D1-D2-D3-Fc2, Obi-Fc1-D1-Fc2, Obi-Fc1-D1-D2-Fc2, Obi-Fc1-D1-D2-D3-Fc2, Anti-EGFR-Fc1-D1-Fc2, Anti-EGFR-Fc1-D1-D2-Fc2, Anti-EGER-Fc1-D1-D2-D3-Fc2, Anti-Her2(P)-Fc1-D1-Fc2, Anti-Her2(P)-Fc1-D1-D2-Fc2, Anti-Her2(P)-Fc1-D1-D2-D3-Fc2, Anti-Her2(T)-Fc1-D1-Fc2, Anti-Her2(T)-Fc1-D1-D2-Fc2, Anti-Her2(T)-Fc1-D1-D2-D3-Fc2, Anti-EGFR-Fc1-D1-Fc2, Anti-EGFR-Fc1-D1-D2-Fc2, Anti-EGFR-Fc1-D1-D2-D3-Fc2, Anti-PD-L1(Ate)-Fc1-D1-Fc2, Anti-PD-L1(Ate)-Fc1-D1-D2-Fc2, Anti-PD-L1(Ate)-Fc1-D1-D2-D3-Fc2, Anti-PD-L1(13G4)-Fc1-D1-Fc2, Anti-PD-L1(13G4)-Fc1-D1-D2-Fc2, Anti-PD-L1(13G4)-Fc1-D1-D2-D3-Fc2, Anti-PD-L1(12A4)-Fc1-D1-Fc2, Anti-PD-L1(12A4)-Fc1-D1-D2-Fc2 and Anti-PD-L1(12A4)-Fc1-D1-D2-D3-Fc2 had a similar result to the above results of Ofa-Fc1-D1-Fc2 at the same dose.

(232) TABLE-US-00011 TABLE 10 Effect of the recombinant protein on the number of RBCs (10.sup.12 cells/L) of cynomolgus monkeys Parallel Before experi- Animal adminis- Group ment identifier tration D 2 D 7 D 14 D 28 1 1 101 4.23 4.15 3.98 5.01 5.66 2 102 4.67 4.19 4.05 4.7 5.16 2 1 201 5.27 4.69 4.04 4.99 5.59 2 202 4.71 4.02 4.26 4.99 5.72

(233) As shown in FIG. 18A and Table 10, Ofa-Fc1-D1-Fc2 does not affect the number of RBCs of cynomolgus monkeys at both dose of 0.5 mg/kg and 5 mg/kg; as shown in FIG. 18B and Table 10, Ofa-Fc1-D1-Fc2 does not affect the hemoglobin of cynomolgus monkeys at both dose of 0.5 mg/kg and 5 mg/kg.

(234) TABLE-US-00012 TABLE 11 Effect of the recombinant protein on the hemoglobin (g/L) of cynomolgus monkeys Parallel Before experi- Animal adminis- Group ment identifier tration D 2 D 7 D 14 D 28 1 1 101 97 97 89 115 129 2 102 109 98 93 108 118 2 1 201 126 110 96 115 129 2 202 107 90 95 112 128

(235) The recombinant proteins Ofa-Fc1-D1-D2-Fc2, Ofa-Fc1-D1-D2-D3-Fc2, Obi-Fc1-D1-Fc2, Obi-Fc1-D1-D2-Fc2, Obi-Fc1-D1-D2-D3-Fe2, Anti-EGFR-Fc1-D1-Fc2, Anti-EGFR-Fc1-D1-D2-Fc2, Anti-EGFR-Fc1-D1-D2-D3-Fc2, Anti-Her2(P)-Fc1-D1-Fc2, Anti-Her2(P)-Fc1-D1-D2-Fc2, Anti-Her2(P)-Fc1-D1-D2-D3-Fc2, Anti-Her2(T)-Fc1-D1-Fc2, Anti-Her2(T)-Fc1-D1-D2-Fc2, Anti-Her2(T)-Fc1-D1-D2-D3-Fc2, Anti-EGFR-Fc1-D1-Fc2, Anti-EGFR-Fc1-D1-D2-Fc2, Anti-EGFR-Fc1-D1-D2-D3-Fc2, Anti-PD-L1(Ate)-Fc1-D1-Fc2, Anti-PD-L1(Ate)-Fc1-D1-D2-Fc2, Anti-PD-L1(Ate)-Fc1-D1-D2-D3-Fc2, Anti-PD-L1(13G4)-Fc1-D1-Fc2, Anti-PD-L1(13G4)-Fc1-D1-D2-Fe2, Anti-PD-L1(13G4)-Fc1-D1-D2-D3-Fe2, Anti-PD-L1(12A-4)-Fc1-D1-Fc2, Anti-PD-L1(12A4)-Fc1-D1-D2-Fc2 and Anti-PD-L1(12A4)-Fc1-D1-D2-D3-Fc2 had a similar effect on the number of RBCs and the amount of hemoglobin of cynomolgus monkeys compared to the above results of Ofa-Fc1-D1-Fc2 at the same dose.

(236) As shown in FIG. 19, based on the test results of the T/B cell typing, Ofa-Fc1-D1-Fc2 can significantly eliminate B cells (the target cell with the CD20 antigen) of the animals at both dose of 0.5 mg/kg and 5 mg/kg.

(237) As known to those skilled in the art, the above test results indicated that, after a single administration of the recombinant proteins of the present disclosure which are capable of simultaneously binding to the target antigen and the CD47 antigen, no significant abnormalities related to the drug were observed in the animals, the food consumption and body weight were all fluctuated within the normal range; compared to the data before administration, the blood coagulation, urine and electrocardiogram data of the animals after administration had no significant changes; after the animals were dissected, all organs were observed to be within the normal range, and the weight of the organs, visceral coefficient, and the visceral-brain ratio were also within the normal range.

(238) As known to those skilled in the art, the above test results indicate that, after a single administration of the recombinant proteins of the present disclosure which are capable of simultaneously binding to the target antigen and the CD47 antigen, the number of RBCs and the amount of hemoglobin of the animal were not affected.

(239) As known to those skilled in the art, the above test results indicate that the recombinant proteins of the present disclosure which are capable of simultaneously binding to the target antigen and the CD47 antigen preferentially clears the cells and/or tumor cells with the tumor-targeting antigen under the condition of a same dose.

EXAMPLE 9

Inhibition of Tumor Growth by the Recombinant Proteins in Vivo

(240) Experiments of the recombinant proteins Ofa-Fc1-D1-Fc2, Ofa-Fc1-D1-D2-Fc2, Ofa-Fc1-D1-D2-D3-Fc2, Obi-Fc1-D1-Fc2, Obi-Fc1-D1-D2-Fc2, Obi-Fc1-D1-D2-D3-Fc2, Anti-EGFR-Fc1-D1-Fc2, Anti-EGFR-Fc1-D1-D2-Fc2, Anti-EGFR-Fc1-D1-D2-D3-Fc2, Anti-Her2(P)-Fc1-D1-Fc2, Anti-Her2(P)-Fc1-D1-D2-Fc2, Anti-Her2(P)-Fc1-D1-D2-D3-Fc2, Anti-Her2(T)-Fc1-D1-Fc2, Anti-Her2(T)-Fc1-D1-D2-Fc2, Anti-Her2(T)-Fc1-D1-D2-D3-Fc2, Anti-EGFR-Fc1-D1-Fc2, Anti-EGFR-Fc1-D1-D2-Fc2, Anti-EGFR-Fc1-D1-D2-D3-Fc2, Anti-PD-L1(Ate)-Fc1-D1-Fc2, Anti-PD-L1(Ate)-Fc1-D1-D2-Fc2, Anti-PD-L1(Ate)-Fc1-D1-D2-D3-Fc2, Anti-PD-L1(13G4)-Fc1-D1-Fc2, Anti-PD-L1(13G4)-Fc1-D1-D2-Fc2, Anti-PD-L1 (13G4)-Fc1-D1-D2-D3-Fc2, Anti-PD-L1 (12A4)-Fc1-D1-Fc2, Anti-PD-L1 (12A4)-Fc1-D1-D2-Fc2, Anti-PD-L1(12A4)-Fc1-D1-D2-D3-Fc2 on inhibition of tumor growth were conducted respectively in vivo. Ofa-Fc1-D1-Fc2 was taken as an example, and the following method is applicable for the recombinant proteins comprising an extracellular truncated variant of SIRPα in the right arm.

(241) Ofa-Fc1-D1-Fc2: a colorless clear liquid with a concentration of 1.14-4.02 mg/mL was aliquoted and stored at −80° C.; Rituxan® (rituximab injection): a colorless clear liquid 100 mg/10 mL, Lot No. H0205, stored at 2-8° C. and protected from light. Preparation Buffer (Tris-citrate, pH 6.5): a colorless clear liquid, stored at 2-8° C.

(242) Formulation: Ofa-Fc1-D1-Fc2 and Rituxan® were diluted with the preparation buffer; the preparation buffer was directly administered as the solvent.

(243) Cell: CD20-positive human B-cell lymphoma Daudi cells were purchased from the Cell Bank of Chinese Academy of Sciences and cultured with RPMI 1640 medium supplemented with 10% fetal bovine serum, penicillin and streptomycin in an incubator containing 5% CO.sub.2 at 37° C. The cells were passaged twice a week, and the cells in the exponential growth phase were collected, counted and inoculated.

(244) Experimental animals: Female NOD-SCID mice, 6-7 weeks, purchased from Shanghai Lingchang Biotech Co., Ltd.; license number: SCXK (Hu) 2013-0018. Animal certificate number: 2013001829463, 2013001827545. Feeding environment: SIT level. The use and welfare of the experimental animals shall comply with the provisions of Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC). The health status and mortality of the animals were monitored daily. Routine monitoring included observing the effects of the test substances and drugs on the daily behavior of the animals such as behavioral activities, weight changes, and appearances.

(245) Each mouse was subcutaneously inoculated with 1.5×10.sup.7 Baudi cells, and when the average tumor volume reached 100-150 mm.sup.3 on the 18th day after the inoculation, the animals were divided into different groups and administered (D0). The mice were intravenously (IV) injected with the drugs; the mice in the control group were injected with a same volume of the solvent; the injection volume was 0.1 mL per 10 g body weight. The dose and dosage regimen refers to Table 12.

(246) The diameter of the tumor was measured twice a week with a vernier caliper. The tumor volume (V) is calculated by the following formula:

(247) The tumor volume (V) is calculated by the following formula:
V=½a×b.sup.2;

(248) wherein a and b represent the length and width respectively.
T/C(%)=(T−T0)/(C−C0)×100;

(249) wherein T and C represent the tumor volume at the end of the experiment; T0 and C0 represent the tumor volume at the start of the experiment; T represents the treatment group, and C represents the control group.
Tumor inhibition rate: (TGI)(%)=100−T/C(%)

(250) When the tumor regresses, the tumor inhibition rate (TGI) (%)=100−(T−T0)/T0×100.

(251) If the tumor volume is smaller than the initial volume, i.e., T<T0 or C<C0, it is defined as tumor partial regression (PR); if the tumor completely disappears, it is defined as tumor complete regression (CR).

(252) When the experiment is over, or when the tumor volume of the animal reached a euthanasia endpoint of 1500 mm.sup.3, the animals were euthanatized by carbon dioxide anesthesia, and then the tumor was taken by dissection and photographed.

(253) The comparison of the tumor volume and weight between the two groups was conducted by two-tailed Students t test, and P<0.05 was defined as a statistically significant difference.

(254) Ofa-Fc1-D1-Fc2 (5 mg/kg, IV, twice a week for 5 times) significantly inhibited the growth of Daudi subcutaneously transplanted tumor, with an inhibition rate of 80.8% and tumor partial regression in 1/6 mice. Rituxan® (7 mg/kg, IV twice a week for 5 times) had a tumor inhibition rate of 24.5% on the Daudi subcutaneously transplanted tumor. Tumor-bearing mice were generally well tolerated to the above drugs (Table 12, FIG. 20, 21, 22).

(255) TABLE-US-00013 TABLE 12 Efficacy of Ofa-Fc1-D1-Fc2 and Rituxan ® on the human B cell lymphoma Daudi subcutaneously transplanted tumor Average Average Animal Animal tumor tumor number number volume volume % (TGI) Partial Complete at the at the Group Administration Route (mm.sup.3) D 0 SEM (mm.sup.3) D 18 SEM T/C (%) P value regression regression start end Solvent D 0, IV 103.3 ±1.7 1558.7 ±374.6 — — — 0 0 6 6 Ofa-Fc1- D 3, 99.4 ±1.7 378.8 ±97.5 19.2 80.8 0.0124 1 0 6 6 D1-Fc2 D 7, Rituxan ®  D 10, 101.6 ±1.0 1200.8 ±175.7 75.5 24.5 0.4089 0 0 6 6 7 mg/kg  D 14 Note: Randomly divided into groups, the first administration time is D 0; IV: intravenous injection.

(256) It can be seen that both Ofa-Fc1-D1-Fc2 and Rituxan® inhibited the growth of CD20-positive human B cell lymphoma Daudi subcutaneous xenograft tumor to varying degrees, wherein Ofa-Fc1-D1-Fc2 is significantly superior to Rituxan®; Mice are generally well tolerated to the above drugs.

(257) The anti-tumor effect of the recombinant proteins Ofa-Fc1-D1-D2-Fc2, Ofa-Fc1-D1-D2-D3-Fc2, Obi-Fc1-D1-Fc2, Obi-Fc1-D1-D2-Fc2, Obi-Fc1-D1-D2-D3-Fc2, Anti-EGFR-Fc1-D1-Fc2, Anti-EGFR-Fc1-D1-D2-Fc2, Anti-EGFR-Fc1-D1-D2-D3-Fc2, Anti-Her2(P)-Fc1-D1-Fc2, Anti-Her2(P)-Fc1-D1-D2-Fc2, Anti-Her2(P)-Fc1-D1-D2-D3-Fc2, Anti-Her2(T)-Fc1-D1-Fc2, Anti-Her2(T)-Fc1-D1-D2-Fc2, Anti-Her2(T)-Fc1-D1-D2-D3-Fc2, Anti-EGFR-Fc1-D1-Fc2, Anti-EGFR-Fc1-D1-D2-Fc2, Anti-EGFR-Fc1-D1-D2-D3-Fc2, Anti-PD-L1(Ate)-Fc1-D1-Fc2, Anti-PD-L1(Ate)-Fc1-D1-D2-Fc2, Anti-PD-L1(Ate)-Fc1-D1-D2-D3-Fc2, Anti-PD-L1(13G4)-Fc1-D1-Fc2, Anti-PD-L1(13G4)-Fc1-D1-D2-Fc2, Anti-PD-L1(13G4)-Fc1-D1-D2-D3-Fc2, Anti-PD-L1(12A4)-Fc1-D1-Fc2, Anti-PD-L1(12A4)-Fc1-D1-D2-Fc2 and Anti-PD-L1(12A4)-Fc1-D1-D2-D3-Fc2 on the transplanted tumor in NOD-SCID mice at the same dose and the tolerance of the mice to the recombinant proteins were similar to the above results of Ofa-Fc1-D1-Fc2.

(258) As known to those skilled in the art, the above test results indicate that the recombinant proteins of the present disclosure which are capable of simultaneously binding to the tumor-targeting antigen and the CD47 antigen have an unexpectedly significant anti-tumor effect compared to the tumor-targeting antibody at the condition of a same dose.

(259) The use and welfare of the experimental animals shall comply with the provisions of Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC), The health status and mortality of the animals were monitored daily. Routine monitoring included observing the effects of the test substances and drugs on the daily behavior of the animals such as behavioral activities, weight changes, and appearances.

(260) The use of any and all examples, or exemplary language (e.g., “such as”) provided herein, is intended merely to better illuminate the present invention and does not pose a limitation on the scope of the present invention unless otherwise claimed. No language in the specification should be construed as indicating any non-claimed element as essential to the practice of the present invention.

(261) All publications and patent applications mentioned in this specification are herein incorporated by reference in their entireties to the same extent as if each individual publication or patent application is specifically and individually indicated to be incorporated by reference. In addition, any theory, mechanism, proof, or finding stated herein is meant to further enhance understanding of the present invention and is not intended to limit the inventions in any way to such theory, mechanism, proof, or finding. While the present invention has been illustrated and described in detail in the drawings and foregoing description, such illustration and description are to be considered illustrative and not restrictive.