Methods for producing compositions containing plasmalogen

Abstract

Provided is a method for producing a composition containing plasmalogen derived from a plasmalogen-containing animal tissue, the method being without significantly decreasing an amount of plasmalogen derived the plasmalogen-containing animal tissue. A method for producing a composition containing plasmalogen derived from an animal tissue, the method comprising: (A) concentrating an alcohol extract of a plasmalogen-containing animal tissue, and (B) after diluting a concentrated product obtained by (A), allowing to stand under refrigeration.

Claims

1. A method for producing a composition containing plasmalogen derived from an animal tissue, the method comprising: (A) concentrating an alcohol extract of a plasmalogen-containing animal tissue, and (B) diluting a concentrated product obtained by (A) to obtain a diluted product, and keeping the diluted product under refrigeration, wherein keeping the diluted product under refrigeration in (B) is carried out at 2 to 15° C.

2. The method according to claim 1, wherein keeping the diluted product under refrigeration of (B) is carried out for 1 to 7 days.

3. The method according to claim 1, wherein the plasmalogen-containing animal tissue is a dried material.

4. The method according to claim 3, wherein the drying is carried out by blowing air at 25 to 59° C. to the animal tissue.

5. The method according to claim 1, wherein the animal tissue is derived from aquatic invertebrates.

6. The method according to claim 5, wherein the aquatic invertebrates are aquatic invertebrates of phylum Chordata, subphylum Urochordata, class Ascidiacea.

7. The method according to claim 6, wherein the aquatic invertebrates of phylum Chordata, subphylum Urochordata, class Ascidiacea are aquatic invertebrates of the genus Halocynthia.

8. The method according to claim 7, wherein the aquatic invertebrates of the genus Halocynthia are Halocynthia roretzi or Halocynthia aurantium.

9. The method according to claim 1, wherein plasmalogen derived from an animal tissue contains a group corresponding to an aliphatic hydrocarbon portion of docosahexaenoic acid or eicosapentaenoic acid.

10. The method according to claim 1, wherein the composition is a food composition or a pharmaceutical composition.

11. The method according to claim 10, wherein the composition is a food composition.

Description

EXAMPLES

(1) In the following, the present invention will be explained in more detail by referring to Examples, but these Examples do not limit the scope of the present invention in any way.

Example 1, Comparative Examples 1 to 3

(2) Halocynthia roretzi with shells was divided into two equal parts, and dried in a cool air dryer by applying air at about 45° C. for about 24 hours (the temperature in the cool air dryer (ambient temperature) becomes about 45° C.). To the dried material was added a mixed solution of ethanol and water (95% by volume:5% by volume), and the mixture was extracted under stirring at about 40° C. for about 2 hours. The solid and liquid were separated, using a stainless strainer (200 mesh), into a solid phase 1 and a liquid phase 1. The liquid phase 1 was subjected to suction filtration to remove the solid material, to obtain a liquid phase 2. To the solid phase 1 was added a mixed solution of ethanol and water (95% by volume:5% by volume). After immersion at room temperature for about 10 hours, the solid and liquid were separated, using a stainless strainer (200 mesh), into a solid phase 2 and a liquid phase 3. The liquid phase 3 was subjected to suction filtration to remove the solid material, to obtain a liquid phase 4. The liquid phase 2 and the liquid phase 4 were combined and concentrated under reduced pressure at about 45° C. for about 24 hours. The concentrated product was stored, under nitrogen bubbling, in a sealed condition at about 5° C. for about 8 days. Thereafter, water was added thereto to dilute the product, and the resulting material was allowed to stand at the temperature shown below for about 3 days. Thereafter, the supernatant was removed by decantation at about 4° C. to collect a liquid phase 5, and a content of plasmalogen in the liquid phase 5 was measured by high performance liquid chromatography.

(3) TABLE-US-00001 TABLE 1 Comparative Comparative Comparative Example 1 Example 1 Example 2 Example 3 Tempera- 2~5 16~20 21~25 26~35 ture (° C.) at standing Plasmalogen 2.46  1.06  0.76  0.13 content (% by mass) in liquid phase

(4) From the results of Example 1 and Comparative Examples 1 to 3, when the standing temperature is 2 to 5° C., as compared with the temperature other than that, it can be understood that plasmalogen content derived from a plasmalogen-containing animal tissue in the liquid phase is higher.

(5) Accordingly, the production method of Example 1 is a method which can produce a composition containing plasmalogen derived from an animal tissue without significantly reducing an amount of plasmalogen derived from a plasmalogen-containing animal tissue.

Example 2, Comparative Examples 4 to 6

(6) Halocynthia roretzi with shells was divided into two equal parts, and dried in a cool air dryer by applying air at a temperature shown in the following Table for about 24 hours (the temperature in the cool air dryer (ambient temperature) becomes substantially the same as the temperature of the air in each Example or Comparative Example). To the dried material was added a mixed solution of ethanol and water (95% by volume:5% by volume), and the mixture was extracted under stirring at about 40° C. for about 2 hours. The solid and liquid were separated, using a stainless strainer (200 mesh), into a solid phase 1 and a liquid phase 1. The liquid phase 1 was subjected to suction filtration to remove the solid material, to obtain a liquid phase 2. To the solid phase 1 was added a mixed solution of ethanol and water (95% by volume:5% by volume). After immersion at room temperature for about 10 hours, the solid and liquid were separated, using a stainless strainer (200 mesh), into a solid phase 2 and a liquid phase 3. The liquid phase 3 was subjected to suction filtration to remove the solid material, to obtain a liquid phase 4. After combining the liquid phase 2 and the liquid phase 4, a content of plasmalogen in the combined liquid phases was measured by high performance liquid chromatography.

(7) TABLE-US-00002 TABLE 2 Comparative Comparative Comparative Example 2 Example 4 Example 5 Example 6 Tempera- 40~50 60 85 100 ture (° C.) of air Plasmalogen  0.49  0.21  0.09  0.02 content (% by mass) in extracted liquid

(8) It can be understood from the results of Example 2 and Comparative Examples 4 to 6 that plasmalogen content derived from a plasmalogen-containing animal tissue in the alcohol extract is higher when drying is carried out by applying air at 40 to 50° C. to an animal tissue, as compared with the conditions other than that.

(9) Accordingly, the production method of Example 2 is a method which can produce a composition containing higher concentration of plasmalogen derived from an animal tissue.

(10) As shown in the results of Examples mentioned above, the present invention can produce a composition containing plasmalogen derived from an animal tissue without significantly reducing an amount of plasmalogen derived from a plasmalogen-containing animal tissue.