Fusarium Resistance in Celery

Abstract

Provided herein are Apium graveolens plants resistant to the plant pathogen Fusarium oxysporum f. sp. Apii race 4 and wherein the resistance is encoded by one genomic region or a combination of at least two, or three genomic regions. Also provided herein are methods for identifying the present Fusarium oxysporum f. sp. Apii race 4 resistant plants and to molecular markers for use in the present methods.

Claims

1. An apium graveolens plant wherein the plant is resistant to the plant pathogen Fusarium oxysporum f. sp. Apii race 4 and wherein the resistance is encoded by a combination of at least two genomic regions, a first genomic region, located on linkage group 4 between 28 and 33 CM; and a second genomic region, located on linkage group 5 between 56 and 60 cM.

2. The apium graveolens plant according to claim 1, wherein the resistance is encoded by a combination of at least three genomic regions, a third genomic region, located on linkage group 7 between 24 and 38 cM.

3. The apium graveolens plant according to claim 1, wherein the genomic regions are obtainable, obtained, or are from an Apium graveolens plant deposited under deposit number NCIMB 43699.

4. The apium graveolens plant according to claim 1, wherein said first genomic region, located on linkage group 4, is characterized by one or more sequences selected from the group consisting of SEQ ID Nos. 1, 3, 5, 7 and 9.

5. The apium graveolens plant according to claim 1, wherein said second genomic region, located on linkage group 5, is characterized by one or more sequences selected from the group consisting of SEQ ID Nos. 11, 13, 15, 17, 19, 21, 23, 25 and 27.

6. The apium graveolens plant according to claim 2, wherein said third genomic region, located on linkage group 7, is characterized by one or more sequences selected from the group consisting of SEQ ID Nos. 29, 31, 33 and 35.

7. The apium graveolens plant according to claim 2, wherein said combination of at least three genomic regions is characterized by SEQ ID Nos. 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33 and 35.

8. The apium graveolens plant according to claim 1, wherein said plant is cytoplasmic male sterile (CMS).

9. The apium graveolens plant according to claim 1, wherein said plant is a hybrid plant.

10. The apium graveolens plant according to claim 1, wherein said plant is an Apium graveolens plant deposited under deposit number NCIMB 43699.

11. The apium graveolens plant according to claim 1, wherein the plant is selected from the group consisting of Apium graveolens var. secalinum, Apium graveolens var. dulce, and Apium graveolens var. rapaceum.

12. A hybrid celery or celeriac obtained by crossing a Fusarium oxysporum susceptible celery or celeriac with an Apium graveolens plant according to claim 1.

13. A method for identifying a genomically encoded resistance against the plant pathogen Fusarium oxysporum f. sp. apii race 4 as found in Apium graveolens plant deposited under deposit number NCIMB 43699, comprising detecting a combination of at least two genomic regions, a first genomic region, located on linkage group 4 between 28 and 33 CM and a second genomic region, located on linkage group 5 between 56 and 60 cM.

14. The method according to claim 13, wherein said first genomic region, located on linkage group 4, is detected using one or more molecular markers selected from the group consisting of SEQ ID Nos. 1, 3, 5, 7 and 9.

15. The method according to claim 13, wherein said second genomic region, located on linkage group 5, is detected using one or more molecular markers selected from the group consisting of SEQ ID Nos. 11, 13, 15, 17, 19, 21, 23, 25 and 27.

16. The method according to claim 13, further comprising detecting a third genomic region, located on linkage group 7 between 24 and 38 cM, using one or more molecular markers selected from the group consisting of SEQ ID Nos. 29, 31, 33 and 35.

17. A seed or plant part of an Apium graveolens plant according to claim 1.

18. A molecular marker selected from the group consisting of SEQ ID Nos. 1 to 36.

Description

DESCRIPTION OF THE INVENTION

[0029] Although the present genomic fragments can be introduced into A. graveolens plants by introgression, these genomic fragments can also be artificially introduced in plant cells to generate Foa4-resistant A. graveolens plants using various genome engineering techniques.

[0030] As the genomic regions are known, these genomic fragments can, for example, be transferred between plants using microplast-mediated chromosome transfer. Using this method, entire chromosomes or parts thereof can be horizontally transferred between plants. First, micro-protoplasts containing one or a few chromosomes that carry the resistance are generated. Subsequently, the micro-protoplasts are fused with protoplasts generated from a susceptible A. graveolens plant. This method produces plants with monosomic additions, which can subsequently be crossed with other plants to generate Foa4-resistant lines.

[0031] Alternatively, as the nucleotide sequences of the present genomic fragments are known, these fragments can also be artificially assembled in yeast and subsequently allowed to recombine with the A. graveolens genome. The genomic fragments can also be amplified by long-range PCR amplifications or de novo synthesized and the resulting fragments can be cloned and transformed into A. graveolens cells in a single step or in a series of transformations ultimately resulting in the present A. graveolens plants. The present genomic fragments, completely or in parts later to be reassembled, can also be isolated from gels or columns, for example, after restriction digestion, and subsequently transformed into A. graveolens cells.

[0032] Yet alternatively, the genomic fragments of interest can be introduced into a vector under a (strong) promotor. Subsequently, susceptible plants can be transformed with the vector and the sequence of interest would be expressed resulting in resistance. These techniques are readily available for the skilled person. Construction of artificial chromosomes comprising the present genomic fragments is also contemplated within the context of the present invention.

[0033] According to a preferred embodiment of the present invention, the present genomic region or regions is/are obtainable, obtained, or is/are from an Apium graveolens plant deposited under deposit number NCIMB 43699. An Apium graveolens plant comprising one genomic region located on linkage group 4 between 28 and 33 cM, one genomic region located on linkage group 5 between 56 and 60 cM, and one genomic region located on linkage group 7 between 24 and 38 cM was deposited at NCIMB (National Collections of Industrial, Food and Marine Bacteria; NCIMB Limited, Ferguson Building; Craibstone Estate, Bucksburn Aberdeen, Scotland, AB21 9YA United Kingdom) on 30 Nov. 2020 under deposit number NCIMB 43699.

[0034] The present Apium graveolens plants preferably comprise in their genome at least one sequence selected from the group consisting of SEQ ID No. 1, SEQ ID No. 3, SEQ ID No. 5, SEQ ID No. 7, and SEQ ID No. 9. The present sequences represent the resistance providing allele while plants comprising in their genome at least one sequence selected from the group consisting of SEQ ID No. 2, SEQ ID No. 4, SEQ ID No. 6, SEQ ID No. 8, and SEQ ID No. 10 comprise the susceptible allele.

[0035] The present Apium graveolens plants preferably further comprise in their genome at least one sequence selected from the group consisting of SEQ ID No. 11, SEQ ID No. 13, SEQ ID No. 15, SEQ ID No. 17, SEQ ID No. 19, SEQ ID No. 21, SEQ ID No. 23, SEQ ID No. 25, and SEQ ID No. 27.

[0036] The present sequences represent the resistance providing allele while plants comprising in their genome at least one sequence selected from the group consisting of SEQ ID No. 12, SEQ ID No. 14, SEQ ID No. 16, SEQ ID No. 18, SEQ ID No. 20, SEQ ID No. 22, SEQ ID No. 24, SEQ ID No. 26, and SEQ ID No. 28 comprise the susceptible allele.

[0037] The present Apium graveolens plants further preferably comprise in their genome at least one sequence selected from the group consisting of SEQ ID No. 29, SEQ ID No. 31, SEQ ID No. 33, and SEQ ID No. 35. The present sequences represent the resistance providing alleles while plants comprising in their genome at least one sequence selected from the group consisting of SEQ ID No. 30, SEQ ID No. 32, SEQ ID No. 34, and SEQ ID No. 36 comprise the susceptible allele.

[0038] The present Apium graveolens plants more preferably comprise in their genome at least one sequence selected from the group consisting of SEQ ID No. 1, SEQ ID No. 3, SEQ ID No. 5, SEQ ID No. 7, SEQ ID No. 9, i.e. linkage group 4, and at least one sequence selected from the group consisting of SEQ ID No. 11, SEQ ID No. 13, SEQ ID No. 15, SEQ ID No. 17, SEQ ID No. 19, SEQ ID No. 21, SEQ ID No. 23, SEQ ID No. 25, SEQ ID No. 27, i.e. linkage group 5 and/or at least one sequence selected from the group consisting of SEQ ID No. 29, SEQ ID No. 31, SEQ ID No. 33, SEQ ID No. 35, i.e. linkage group 7. The present sequences present the resistance providing alleles while plants comprising in their genome at least one sequence selected from the group consisting of SEQ ID No. 2, SEQ ID No. 4, SEQ ID No. 6, SEQ ID No. 8, and SEQ ID No. 10 and at least one sequence selected from the group consisting of SEQ ID No. 12, SEQ ID No. 14, SEQ ID No. 16, SEQ ID No. 18, SEQ ID No. 20, SEQ ID No. 22, SEQ ID No. 24, SEQ ID No. 26, and SEQ ID No. 28 and/or at least one sequence selected from the group consisting of SEQ ID No. 30, SEQ ID No. 32, SEQ ID No. 34, and SEQ ID No. 36 comprise susceptible alleles.

[0039] The present Apium graveolens plants most preferably comprise in their genome at least one sequence selected from the group consisting of SEQ ID No. 1, SEQ ID No. 3, SEQ ID No. 5, SEQ ID No. 7, SEQ ID No. 9, i.e. linkage group 4, and at least one sequence selected from the group consisting of SEQ ID No. 11, SEQ ID No. 13, SEQ ID No. 15, SEQ ID No. 17, SEQ ID No. 19, SEQ ID No. 21, SEQ ID No. 23, SEQ ID No. 25, SEQ ID No. 27, i.e. linkage group 5 and at least one sequence selected from the group consisting of SEQ ID No. 29, SEQ ID No. 31, SEQ ID No. 33, SEQ ID No. 35, i.e. linkage group 7. The present sequences present the resistance providing alleles while plants comprising in their genome at least one sequence selected from the group consisting of SEQ ID No. 2, SEQ ID No. 4, SEQ ID No. 6, SEQ ID No. 8, and SEQ ID No. 10 and at least one sequence selected from the group consisting of SEQ ID No. 12, SEQ ID No. 14, SEQ ID No. 16, SEQ ID No. 18, SEQ ID No. 20, SEQ ID No. 22, SEQ ID No. 24, SEQ ID No. 26, and SEQ ID No. 28 and at least one sequence selected from the group consisting of SEQ ID No. 30, SEQ ID No. 32, SEQ ID No. 34, and SEQ ID No. 36 comprise susceptible alleles.

[0040] The present Apium graveolens plants preferably comprise in their genome at least one sequence selected from the group consisting of SEQ ID No. 11, SEQ ID No. 13, SEQ ID No. 15, SEQ ID No. 17, SEQ ID No. 19, SEQ ID No. 21, SEQ ID No. 23, SEQ ID No. 25, SEQ ID No. 27, i.e. linkage group 5 and at least one sequence selected from the group consisting of SEQ ID No. 29, SEQ ID No. 31, SEQ ID No. 33, SEQ ID No. 35, i.e. linkage group 7. The present sequences present the resistance providing alleles while plants comprising in their genome at least one sequence selected from the group consisting of SEQ ID No. 12, SEQ ID No. 14, SEQ ID No. 16, SEQ ID No. 18, SEQ ID No. 20, SEQ ID No. 22, SEQ ID No. 24, SEQ ID No. 26, and SEQ ID No. 28 and at least one sequence selected from the group consisting of SEQ ID No. 30, SEQ ID No. 32, SEQ ID No. 34, and SEQ ID No. 36 comprise susceptible alleles.

[0041] According to a preferred embodiment, the present Apium graveolens plants are cytoplasmic male sterile (CMS).

[0042] According to yet another preferred embodiment, the present Apium graveolens plants are hybrid plants.

[0043] Preferably, the present Apium graveolens plants are selected from the group consisting of Apium graveolens var. secalinum, Apium graveolens var. dulce, and Apium graveolens var. rapaceum.

[0044] The present invention also relates to hybrid celery or celeriac obtainable by crossing Fusarium oxysporum f. sp. apii susceptible celery or celeriac with the present Apium graveolens plants or hybrid celery or celeriac obtainable by crossing a Fusarium oxysporum f. sp. apii susceptible celery or celeriac with deposit NCIMB 43699.

[0045] The present invention further relates to a methods for identifying a genomically encoded resistance against the plant pathogen Fusarium oxysporum f. sp. apii race 4 as found in Apium graveolens plant deposited under deposit number NCIMB 43699, the method comprises the step of detecting one genomic region, a combination of at least two genomic regions or a combination of at least three genomic regions, one genomic region is located on linkage group 4 between 28 and 33 cM; one genomic region is located on linkage group 5 between 56 and 60 cM; and one genomic region is located on linkage group 7 between 24 and 38 cM.

[0046] The present invention further also relates to seeds or plant parts of plants defined above or to seeds capable of providing the present plants and to molecular markers which markers co-segregate with a genomically-encoded resistance against the plant pathogen Fusarium oxysporum f. sp. apii as present in deposit NCIMB 43699.

[0047] The present invention furthermore relates to molecular markers which markers co-segregate with a genomically encoded resistance/susceptibility against the plant pathogen Fusarium oxysporum f. sp. apii as present in deposit NCIMB 43699, which molecular markers are selected from the group consisting of SEQ ID No. 1 until 10, SEQ ID No.'s linkage group 11 until 28 and SEQ ID No.'s 29 until 36.

EXAMPLES

Example 1. General Protocol for Assessing Resistance

[0048] The pathogen Fusarium oxysporum f. sp. apii race 4 (hereafter Foa4) is stored in glycerol at ?80? C. Two weeks before the start of the disease trial, isolates are taken from the ?80? C. and grown on potato dextrose agar (PDA) plates at 25? C. Five days before inoculation, the Foa4 isolates are transferred to liquid Czapek-Dox yeast (CDBY) broth and incubated at 25? C. One day before inoculation, the CDBY Foa4 spore suspension is centrifuged and resuspended in water. Spore concentration is adjusted to 106 spores/ml.

[0049] Per genotype, twenty 4-week-old seedlings are transplanted in 10?10 cm square pots containing soil. The plants are then grown at 20? C. with a 16 h/8 h light/dark period under greenhouse conditions. As a control, the Foa4-susceptible varieties Daybreak and Golden Spartan were used.

[0050] The celery seedlings are inoculated with the Foa4 spore suspension by injection. Six-week-old seedlings are injected with 5 ml spore suspension. Inoculation is repeated after one week. After the first inoculation, the temperature in the greenhouse is raised to 25? C., while maintaining a 16 h/8 h light/dark period.

[0051] Assessment of the plants occurs 7 weeks after the first inoculation. Plants are cut to determine the degree of internal browning. Plants are scored from 0 to 9. A score of 0 means the plant died and, therefore, is susceptible. A score of 9 means the plant does not have any internal browning and, therefore, is resistant.

Example 2. Results of Assessment for Resistance Against Fusarium oxysporum f Sp. Apii Race 4

[0052]

TABLE-US-00001 Cultivar Score for Foa2 Score for Foa4 Golden Spartan 0.7 0 Daybreak 0 0 Merengo 9 0.9 NCIMB 43699 9 7

Example 3. Production of F1 Seed Applying CMS

[0053] One of the requisites for a modern hybrid variety is that inbreeding, resulting in off type plants, is minimized. In celery, a reliable system for hybrid production is available based on cytoplasmic male sterility. Applying this feature for seed production with male and female parent lines, hybrids essentially are resulting 100% from pure cross pollinations.

Example 4 Molecular Characterization of Genomically Encoded Resistance Against Fusarium oxysporum f. Sp. Apii Race 4 in Apium graveolens

[0054] An F1S1 population was made by crossing the source of resistance to a susceptible celery line, after which the resulting F1 plant was self-pollinated.

[0055] At least 2000 seeds were harvested from the F1S1 generation of a cross between the distinctive source of resistance and a susceptible celery line. To perform a QTL mapping, 900 plants of the cross were grown in the glasshouse and tested for Foa4 resistance. From each individual plant, leaf material was used for DNA isolation and successive marker analysis.

[0056] Using SNP markers covering the entire genome, QTLs were found on linkage group 4 (LG4), linkage group 5 (LG5), and linkage group 7 (LG7). These QTLs are defined by the SNP markers listed in the tables below.

TABLE-US-00002 Position Allele Internal Linkage on Linkage linked Alternative SNP Pat_ID Group Group (cM) to resistance allele 1 5855 4 28.361 G A 2 3253 4 30.397 C T 3 0172 4 30.640 C G 4 2779 4 31.691 A G 5 6114 4 32.369 G A 6 2283 5 56.793 A G 7 1764 5 57.173 T C 8 1049 5 57.670 C A 9 4340 5 58.216 C T 10 5397 5 58.292 T A 11 5832 5 58.371 C T 12 4999 5 58.530 G A 13 2817 5 58.971 C T 14 3869 5 59.024 G A 15 0386 7 24.982 C T 16 0654 7 25.330 T C 17 4676 7 27.722 G A 18 1133 7 37.668 C T

TABLE-US-00003 Positionon SEQ linkage Sequence IDNo. LG* group(cM) -SNPnucleotideishighlightedboldandinbrackets SEQID 4 28.361 GCTGTTGGGTGGGAGAGAGTATGGAAGAGTTTACAAAGGATGG No.1 GTTCAGG[G]GAAGACGTACACCCCGTCTAGGCACGATGTTGGAA CAACCATTGCAGTTA SEQID 4 28.361 GCTGTTGGGTGGGAGAGAGTATGGAAGAGTTTACAAAGGATGG No.2 GTTCAGG[A]GAAGACGTACACCCCGTCTAGGCACGATGTTGGAA CAACCATTGCAGTTA SEQID 4 30.397 AACAGGAACCACACGTGGTTTCCCTTCATGAGAAACAGGACCATT No.3 AGGTG[C]CTTCTGTTTATGACATGAAGCAGGAATATTAGTTGGCT TAGGTTTCTTAG SEQID 4 30.397 AACAGGAACCACACGTGGTTTCCCTTCATGAGAAACAGGACCATT No.4 AGGTG[T]CTTCTGTTTATGACATGAAGCAGGAATATTAGTTGGCT TAGGTTTCTTAG SEQID 4 30.640 TGTTTAGAGGCAAGAAATCAAGAAAAGATGAAATCTTGGGGATT No.5 TTTAGT[C]TGTGTTTGTSTGTGTGTGAATTGGGTTGGAGTGAATTC TTGGTAAAAAGA SEQID 4 30.640 TGTTTAGAGGCAAGAAATCAAGAAAAGATGAAATCTTGGGGATT No.6 TTTAGT[G]TGTGTTTGTSTGTGTGTGAATTGGGTTGGAGTGAATTC TTGGTAAAAAGA SEQID 4 31.691 ACGACAAATTACTACAGTTTGCTTCAATCTTGCCAAATCGTGTGAA No.7 GCTA[A]CACATGTTGAAATACCATGTCCTTTTCTATTTTTAAAGTTA TCGTTTTAG SEQID 4 31.691 ACGACAAATTACTACAGTTTGCTTCAATCTTGCCAAATCGTGTGAA No.8 GCTA[G]CACATGTTGAAATACCATGTCCTTTTCTATTTTTAAAGTT ATCGTTTTAG SEQID 4 32.369 GTGTTAGAGTTAACAAAGTTGGCAGAAACCAAGTGCACTGATCCA No.9 ACGTT[G]TGGGCTAYAGAAGTGTCAACTACTTTAAACTCAGCTGC AGTGGACTTGCC SEQID 4 32.369 GTGTTAGAGTTAACAAAGTTGGCAGAAACCAAGTGCACTGATCCA No.10 ACGTT[A]TGGGCTAYAGAAGTGTCAACTACTTTAAACTCAGCTGC AGTGGACTTGCC SEQID 5 56.793 CACAAACCCCCCTTCATTTCATTTCTCATTTCTCATTTCTCATTTCTC No.11 AC[A]ATTCACTCAACTCTCTCGTAATCTCCGATCCCAATTTCACCCC CACAATG SEQID 5 56.793 CACAAACCCCCCTTCATTTCATTTCTCATTTCTCATTTCTCATTTCTC No.12 AC[G]ATTCACTCAACTCTCTCGTAATCTCCGATCCCAATTTCACCCC CACAATG SEQID 5 57.173 AAGTACCTGTTCTAGTGAGTTTATCTACCTCCTGTCAATCTTTTTCC No.13 TTT[T]TTGTTTTCTGGTACTTGGACCAAAACCCCAAGTGTATAAAC GTCAGGTCT SEQID 5 57.173 AAGTACCTGTTCTAGTGAGTTTATCTACCTCCTGTCAATCTTTTTCC No.14 TTT[C]TTGTTTTCTGGTACTTGGACCAAAACCCCAAGTGTATAAAC GTCAGGTCT SEQID 5 57.670 GGGACATAGCATTCAGGATTTATAAGAGTCTGGAAAGTGCTATTT No.15 TCACC[C]CCCACAAGATCCTGAAGTGCAATCTCAATAATTCTTTCA TTCTCCCTGCT SEQID 5 57.670 GGGACATAGCATTCAGGATTTATAAGAGTCTGGAAAGTGCTATTT No.16 TCACC[A]CCCACAAGATCCTGAAGTGCAATCTCAATAATTCTTTCA TTCTCCCTGCT SEQID 5 58.216 ATAGAGAAGGATATAACAAAATATTGGAAAATGCCAAGCCCCGA No.17 AATGAT[C]CATATGGGAGGCATTTATCTGCCTTTACCTACCTCCGG CTCAGCTCCTTG SEQID 5 58.216 ATAGAGAAGGATATAACAAAATATTGGAAAATGCCAAGCCCCGA No.18 AATGAT[T]CATATGGGAGGCATTTATCTGCCTTTACCTACCTCCGG CTCAGCTCCTTG SEQID 5 58.292 TACCATATGACTGTGCCGAGGGAGGAAATGCAAGCAGAAGTCCT No.19 AGTTTT[T]AAATTCCAAGGTGTGGCAGTAGTCCCAGTGAGTGTCG TATTGTAGATTTT SEQID 5 58.292 TACCATATGACTGTGCCGAGGGAGGAAATGCAAGCAGAAGTCCT No.20 AGTTTT[A]AAATTCCAAGGTGTGGCAGTAGTCCCAGTGAGTGTCG TATTGTAGATTTT SEQID 5 58.371 ATCTCCAGAGTCGTTGCCACAGCTGGCAGAGATTGCTAGGAATCC No.21 TGCTG[C]TTTATCTGAACTTACTGTTGGGAAAGAGGATAACTTGA GACATTCTAGAG SEQID 5 58.371 ATCTCCAGAGTCGTTGCCACAGCTGGCAGAGATTGCTAGGAATCC No.22 TGCTG[T]TTTATCTGAACTTACTGTTGGGAAAGAGGATAACTTGA GACATTCTAGAG SEQID 5 58.530 CAGAAATATGATGCTTAGATATGGTCTTACTGTTACCATACTATTA No.23 ACCC[G]TTAAAAGCAAATATGTTAGGAGACCAATTACAACAAAAA GATCACCACCA SEQID 5 58.530 CAGAAATATGATGCTTAGATATGGTCTTACTGTTACCATACTATTA No.24 ACCC[A]TTAAAAGCAAATATGTTAGGAGACCAATTACAACAAAAA GATCACCACCA SEQID 5 58.971 TTAAAGCCTCTTATGCAACCAAGTAACAAAAATCAAAAGTGAGGA No.25 TTCGA[C]CTAATTGGATTTGAATTTGGGAGTTTTGAAATCTAAGGT TGAATTTGGGG SEQID 5 58.971 TTAAAGCCTCTTATGCAACCAAGTAACAAAAATCAAAAGTGAGGA No.26 TTCGA[T]CTAATTGGATTTGAATTTGGGAGTTTTGAAATCTAAGGT TGAATTTGGGG SEQID 5 59.024 ATATCCCCTTGGGTGGAGCTACTCTTAGTTGCGCAGCACTTATTTA No.27 GCTT[G]TTTTGGAAGGTGTCCAAAAACAAATTCATTAGGTCTGCTT AGCAAAGCCC SEQID 5 59.024 ATATCCCCTTGGGTGGAGCTACTCTTAGTTGCGCAGCACTTATTTA No.28 GCTT[A]TTTTGGAAGGTGTCCAAAAACAAATTCATTAGGTCTGCTT AGCAAAGCCC SEQID 7 24.982 TTTTGTGCGGATTGTAGTAAAAAACTTGGCTGTCAAGTATTCGAG No.29 TACTT[C]RAAAAATTCAGACACTTCATTTTACTGCAAGATGAAGAT CAAGAATCTCG SEQID 7 24.982 TTTTGTGCGGATTGTAGTAAAAAACTTGGCTGTCAAGTATTCGAG No.30 TACTT[T]RAAAAATTCAGACACTTCATTTTACTGCAAGATGAAGAT CAAGAATCTCG SEQID 7 25.330 TAATGAGCGTGAGGTGGAAGCAGCTGGGAATGGAAATTTAACCT No.31 CCAATG[T]AGAAAAGAGTTCTAATGGTCAATTAGAAACAAAAAA SEQID 7 25.330 TAATGAGCGTGAGGTGGAAGCAGCTGGGAATGGAAATTTAACCT No.32 CCAATG[C]AGAAAAGAGTTCTAATGGTCAATTAGAAACAAAAAA SEQID 7 27.722 GCAAAAGTAGGCTCCTGACATTGTACTTATATAAATTGCACTTTAC No.33 TGAA[G]GCGTAATTCTTCTACTACTAATCTGGAATCCAGTCTAAAG AGCCACTGTG SEQID 7 27.722 GCAAAAGTAGGCTCCTGACATTGTACTTATATAAATTGCACTTTAC No.34 TGAA[A]GCGTAATTCTTCTACTACTAATCTGGAATCCAGTCTAAAG AGCCACTGTG SEQID 7 37.668 AAGGAAGAAATCACCAGTGGCYTCAGCAAATGACTTTTTGGGAG No.35 GTGGGG[C]AGGAGAGAGATTTGGGGGAGGTGAAGATGAGGCTT TGATGAGGTGGAGGT SEQID 7 37.668 AAGGAAGAAATCACCAGTGGCYTCAGCAAATGACTTTTTGGGAG No.36 GTGGGG[T]AGGAGAGAGATTTGGGGGAGGTGAAGATGAGGCTT TGATGAGGTGGAGGT *LG-linkage group

Deposit Information

[0057] A sample of A. graveolens 1960176 with resistance to Fusarium oxysporum as described herein was deposited at the NCIMB (National Collections of Industrial, Food and Marine Bacteria (NCIMB), NCIMB Limited, Ferguson Building; Craibstone Estate, Bucksburn Aberdeen, Scotland, AB21 9YA United Kingdom) on Nov. 30, 2020 under number NCIMB 43699.