COATING FOR MEDICAL DEVICES

Abstract

A coating for a medical device is described. The coating comprises: a base layer comprising a protein; and a polymer layer disposed on the base layer. The polymer layer comprises a polymer having a plurality of hemocompatible groups. Each hemocompatible group is independently selected from a sulfonic acid group, a sulfonamide group, a sulfamic acid group, a hydrogen sulfate group and a conjugate base thereof. Also described is a medical device comprising the coating, and uses and methods involving the coating and the medical device.

Claims

1. A coating for a medical device comprising: a base layer comprising a protein; and a polymer layer disposed on the base layer; wherein the polymer layer comprises a polymer having a plurality of hemocompatible groups, wherein each hemocompatible group is independently selected from a sulfonic acid group, a sulfonamide group, a sulfamic acid group, a hydrogen sulfate group and a conjugate base thereof.

2. The coating according to claim 1, wherein the polymer layer is conjugated to the base layer.

3. The coating according to claim 1, wherein the protein is albumin.

4. The coating according to claim 1, wherein each hemocompatible group is selected from a sulfonic acid group or a sulfonate group.

5. The coating according to claim 1, wherein the polymer having a plurality of hemocompatible groups comprises a repeating unit represented by formula (A-1): ##STR00013## wherein: R.sup.A is selected from H and C.sub.1-6 alkyl; R.sup.B is selected from H and C.sub.1-6 alkyl; X.sup.1 is represented by formula (S-1): ##STR00014## wherein: Z.sup.1 is selected from O, NH and NR.sup.1; R.sup.1 is selected from C.sub.1-6 alkyl, C.sub.6-10 aryl and C.sub.6-10 aryl-C.sub.1-6 alkyl; Y.sup.1 is selected from O.sup.?, OH, NH and NH.sub.2; L.sup.1 is represented by formula (L-1): ##STR00015## wherein: P.sup.1 is selected from a single bond, C.sub.1-10 alkylene, C.sub.2-10 alkenylene, C.sub.2-10 alkynylene and phenylene; Q.sup.1 is selected from a single bond, O, NH, NR.sup.2 and phenylene; and W.sup.1 is selected from a single bond, C.sub.1-10 alkylene, C.sub.2-10 alkenylene, C.sub.2-10 alkynylene and phenylene; and R.sup.2 is selected from C.sub.1-6 alkyl, C.sub.6-10 aryl and C.sub.6-10 aryl-C.sub.1-6 alkyl.

6. The coating according to claim 5, wherein R.sup.A is selected from H and methyl.

7. The coating according to claim 5, wherein R.sup.B is selected from H and methyl.

8. The coating according to claim 5, wherein Z.sup.1 is selected from O and NH.

9. The coating according to claim 5, wherein Y.sup.1 is selected from O.sup.? and OH.

10. The coating according to claim 5, wherein P.sup.1 is selected from C.sub.1-10 alkylene, C.sub.2-10 alkenylene and C.sub.2-10 alkynylene; Q.sup.1 is a single bond and W.sup.1 is a single bond.

11. The coating according to claim 5, wherein P.sup.1 is selected from C.sub.1-10 alkylene, C.sub.2-10 alkenylene and C.sub.2-10 alkynylene; Q.sup.1 is selected from O, NH and NR.sup.2 and W.sup.1 is a single bond.

12. The coating according to claim 1, wherein the polymer having a plurality of hemocompatible groups comprises a repeating unit represented by formula (B-1): ##STR00016## wherein: R.sup.C is selected from H and C.sub.1-6 alkyl; R.sup.D is selected from H, C.sub.1-6 alkyl and C(O)X.sup.2; each X.sup.2 is independently selected from O.sup.?, OH, OR.sup.3, NH.sub.2, NHR.sup.3 and N(R.sup.3).sub.2; and each R.sup.3 is independently selected from optionally substituted C.sub.1-6 alkyl, optionally substituted C.sub.6-10 aryl and optionally substituted C.sub.6-10 aryl-C.sub.1-6 alkyl.

13. The coating according to claim 12, wherein R.sup.C is selected from H and methyl.

14. The coating according to claim 12, wherein R.sup.D is selected from H and methyl.

15. The coating according to claim 12, wherein X.sup.2 is selected from O.sup.? and OH.

16. The coating according to claim 1, wherein the polymer having a plurality of hemocompatible groups is a copolymer.

17. A medical device having a surface coated with a coating according to claim 1.

18. A method of manufacturing a coating for a medical device comprising: (a) applying a polymer having a plurality of hemocompatible groups onto a base layer to form a polymer layer, wherein each hemocompatible group is independently selected from a sulfonic acid group, a sulfonamide group, a sulfamic acid group, a hydrogen sulfate group and a conjugate base thereof; and the base layer comprises a protein.

19. The method of claim 18 further comprising: (b) coupling the polymer having a plurality of hemocompatible groups to the protein using a coupling agent.

20. The method of claim 18, wherein the protein is albumin.

21-37. (canceled)

Description

DETAILED DESCRIPTION

[0033] The invention provides a coating for a medical device. The coating reduces or prevents the clotting of blood. The coating is typically an antithrombotic coating, such as an anticoagulant coating and/or an antiplatelet coating, preferably an antiplatelet coating. The coating is typically a hemocompatible coating.

[0034] Any reference to blood as used herein generally refers to the blood of a human or a non-human animal, such as a mammalian animal. The invention can be used in veterinary applications. The term blood preferably refers to the blood of a human.

[0035] Typically, the coating is biocompatible, preferably biocompatible and/or hemocompatible.

[0036] Generally, the coating comprises a surface layer. The surface layer is the topmost layer of the coating. Thus, the surface layer may be an outer surface layer of the coating or a coated medical device. In use, the surface layer of the coating comes into contact with blood.

[0037] The surface layer or the coating (e.g. as a whole) may be in the form of a gel, preferably a hydrogel. The hydrogel may provide the medical device with a lubricious surface.

[0038] In general, the surface layer is biocompatible.

[0039] The surface layer comprises, or may consist essentially of, the polymer having a plurality of hemocompatible groups, as described herein. When the coating comprises a total of two layers, then the surface layer is the polymer layer.

[0040] Each hemocompatible group is selected from a sulfonic acid group, a sulfonamide group, a sulfamic acid group, a hydrogen sulfate group and a conjugate base thereof. The structure of each group is shown below, where the squiggly line indicates the point of attachment of the group to the polymer, either directly or indirectly.

##STR00001##

[0041] In general, it is preferred that each hemocompatible group is independently selected from a sulfonic acid group, a sulfamic acid group, a hydrogen sulfate group and a conjugate base thereof. More preferably, each hemocompatible group is independently selected from a sulfonic acid group or a sulfonate group (i.e. the conjugate base of a sulfonic acid group).

[0042] The conjugate base of each group is shown below. The conjugate base of each group refers to a sulfonate group, sulfonamide anion group, a sulfamate group or a sulfate group respectively.

##STR00002##

[0043] The plurality of hemocompatible groups is preferably the same hemocompatible group and/or a conjugate base thereof.

[0044] Typically, the plurality of hemocompatible groups is selected from a sulfonic acid group or a conjugate base thereof; a sulfonamide group or a conjugate base thereof; a sulfamic acid group or a conjugate base thereof; and a hydrogen sulfate group or a conjugate base thereof. More preferably, the plurality of hemocompatible groups is selected from a sulfonic acid group or a sulfonate group (i.e. the conjugate base of a sulfonic acid group).

[0045] Without wishing to be bound by theory, it is believed that when the hemocompatible groups described herein are ionised, then the negatively charged conjugate base (e.g. sulfonate group etc) repels the adhesion of platelets, thereby inhibiting or preventing the formation of a blood clot on a surface of the coating. The examples in International patent application no. PCT/EP2020/081383, which are incorporated herein by reference, demonstrate that the hemocompatible groups (referred to as anti-clotting groups in the International patent application) have activity in reducing or preventing blood clots. The polymer of the invention may provide a high density of hemocompatible groups because of the relatively small molecular size of the repeating unit.

[0046] The polymer comprising the plurality of hemocompatible groups has a polymer chain. The polymer chain is the backbone of the polymer. It is preferred that the polymer chain is biocompatible.

[0047] Generally, each hemocompatible group is attached or bonded to the polymer chain by a linker group. Thus, the polymer comprises a plurality of linker groups.

[0048] In principle, any linker group may be used to connect the hemocompatible group to the polymer chain. However, certain linker groups may provide, or contribute to, the advantageous properties of the coating.

[0049] It is believed that the linker group should be relatively short (e.g. 1 to 3 atoms in length) to ensure that the surface of the coating presents a high negative charge density to platelets. When longer linker groups are used, then it is possible for the surface of the coating to have a disordered arrangement of hemocompatible groups due to the conformational flexibility of the linker group. This can reduce the negative charge density provided by the surface of the coating.

[0050] In general, it is preferred that the linker group is biocompatible.

[0051] Each linker group covalently bonds a hemocompatible group to the polymer chain. Thus, the linker group may have a first end that is covalently bonded to the polymer chain and a second end that is covalently bonded to a hemocompatible group.

[0052] A single hemocompatible group may be attached or bonded by a single linker group to the polymer chain.

[0053] The polymer chain may be a linear polymer chain or a branched polymer chain. It is preferred that the polymer chain is a linear polymer chain.

[0054] The polymer or polymer chain typically has a number average (e.g. mean) molecular weight (Mn) of at least 300 g/mol, such as 300 to 50,000 g/mol, preferably 500 to 30,000 g/mol, more preferably 1,000 to 10,000 g/mol. The number average (e.g. mean) molecular weight (Mn) can be determined by light scattering.

[0055] The polymer having a plurality of hemocompatible groups may comprise a repeating unit represented by formula (A 1):

##STR00003##

wherein: [0056] R.sup.A is selected from H and C.sub.1-6 alkyl; [0057] R.sup.B is selected from H and C.sub.1-6 alkyl; [0058] X.sup.1 is represented by formula (S-1):

##STR00004##

and wherein: [0059] Z.sup.1 is selected from O, NH and NR.sup.1; [0060] R.sup.1 is selected from C.sub.1-6 alkyl, C.sub.6-10 aryl and C.sub.6-10 aryl-C.sub.1-6 alkyl; [0061] Y.sup.1 is selected from O, OH, NH.sup.? and NH.sub.2; [0062] L.sup.1 is represented by formula (L-1):

##STR00005##

and wherein: [0063] P.sup.1 is selected from a single bond, C.sub.1-10 alkylene, C.sub.2-10 alkenylene, C.sub.2-10 alkynylene and phenylene; [0064] Q.sup.1 is selected from a single bond, O, NH, NR.sup.2 and phenylene; [0065] W.sup.1 is selected from a single bond, C.sub.1-10 alkylene, C.sub.2-10 alkenylene, [0066] C.sub.2-10 alkynylene and phenylene; and [0067] R.sup.2 is selected from C.sub.1-6 alkyl, C.sub.6-10 aryl and C.sub.6-10 aryl-C.sub.1-6 alkyl.

[0068] The repeating unit represented by formula (A-1) may be a first repeating unit.

[0069] For convenience, the term first repeating unit, particularly the reference to first, is used as a label to distinguish this repeating unit from other types of repeating unit. The use of this term does not require the first repeating unit to be present when reference is made, for example, to a second repeating unit or to a third repeating unit etc. The same also applies to the terms second repeating unit, third repeating unit etc.

[0070] The repeating unit represented by formula (A-1) above may, for example, be obtained or is obtainable from a monomer of formula (a-1) as described herein, such as 2-acrylamido-2-methyl-1-propane sulfonic acid (AMPS), 3-(acryloyloxy)propane-1-sulfonic acid or 3-(methacryloyloxy)-propane-1-sulfonic acid. These monomers are commercially available.

[0071] In formula (A-1), the moiety -L.sup.1- represents the linker group.

[0072] In general, any layer, such as the polymer layer and/or the surface layer, that has a polymer comprising a repeating unit represented by formula (A-1) will typically comprise a polymer composition. The polymer composition has a distribution of molecules of the polymer, where each molecule of polymer includes a repeating unit (e.g. in varying numbers) as represented by formula (A-1) as described herein.

[0073] The repeating unit represented by formula (A-1) may be further represented by formula (A-2) below.

##STR00006##

[0074] In formula (A-2), n.sub.1 is an integer greater than 0, and each of R.sup.A, R.sup.B and X.sup.1 are as defined herein.

[0075] In formula (A-1) or formula (A-2), it is preferred that R.sup.A is selected from H and C.sub.1-3 alkyl, preferably R.sup.A is selected from H and methyl. Even more preferably, R.sup.A is H.

[0076] In formula (A-1) or formula (A-2), it is preferred that R.sup.B is selected from H and C.sub.1-3 alkyl, preferably R.sup.B is selected from H and methyl. Even more preferably, R.sup.B is H.

[0077] In formula (A-1) or formula (A-2), when Z.sup.1 is NR.sup.1, then it is preferred that R.sup.1 is C.sub.1-6 alkyl, particularly methyl or ethyl.

[0078] Typically, Z.sup.1, in formula (A-1) or formula (A-2), is preferably selected from O and NH. Z.sup.1 may be O. More preferably, Z.sup.1 is NH. When Z.sup.1 is O or NH, then the linker group is attached to the polymer chain by an ester or amide group, which is typically a biocompatible group. Amide groups, in particular, are biocompatible because they are present in peptides.

[0079] P.sup.1 is typically selected from a single bond, C.sub.1-10 alkylene and phenylene; and W.sup.1 may be selected from a single bond, C.sub.1-10 alkylene and phenylene. More preferably, P.sup.1 may be selected from a single bond and C.sub.1-10 alkylene; and W.sup.1 may be selected from a single bond and C.sub.1-10 alkylene.

[0080] Alternatively, P.sup.1 is selected from C.sub.1-10 alkylene, C.sub.2-10 alkenylene and C.sub.2-10 alkynylene; Q.sup.1 is selected from O, NH and NR.sup.2 and W.sup.1 is a single bond. More preferably Q.sup.1 is selected from O and NH. In this arrangement, the hemocompatible group is a sulfamate group, a sulfate group or a conjugate base thereof.

[0081] Typically, for L.sup.1 it is preferred that at least one of P.sup.1, Q.sup.1 and W.sup.1 is not a single bond.

[0082] When Q.sup.1 is O, it is generally preferred that P.sup.1 is not a single bond. More preferably, when Q.sup.1 is O, it is preferred that P.sup.1 is C.sub.1-10 alkylene, more preferably P.sup.1 is C.sub.2-4 alkylene.

[0083] When Q.sup.1 is O and P.sup.1 is not a single bond, it may be preferable that W.sup.1 is not a single bond. More preferably, when Q.sup.1 is O, it is preferred that P.sup.1 is C.sub.1-10 alkylene and W.sup.1 is C.sub.1-10 alkylene, more preferably P.sup.1 is C.sub.2-4 alkylene and W.sup.1 is C.sub.2-4 alkylene.

[0084] When Q.sup.1 is phenylene, it is generally preferred that P.sup.1 is a single bond or C.sub.1-10 alkylene and W.sup.1 is a single bond or C.sub.1-10 alkylene. More preferably, when Q.sup.1 is phenylene, it is preferred that P.sup.1 is a single bond or C.sub.1-3 alkylene and W.sup.1 is a single bond or C.sub.1-3 alkylene.

[0085] In general, it is preferred that P.sup.1 is selected from C.sub.1-10 alkylene, C.sub.2-10 alkenylene and C.sub.2-10 alkynylene; Q.sup.1 is a single bond and W.sup.1 is a single bond. More preferably, P.sup.1 is C.sub.1-10 alkylene, particularly C.sub.2-6 alkylene, such as C.sub.2-5 alkylene. Even more preferably P.sup.1 is butylene, propylene or ethylene, preferably iso-butylene.

[0086] When P.sup.1 is ethylene and both Q.sup.1 and W.sup.1 are single bonds, then the moiety represented by (S-1) can be formed from taurine.

[0087] The repeating unit represented by formula (A-1) or formula (A-2) may be anionic, such as when Y.sup.1 is O or NH. When the polymer chain is anionic, then Na.sup.+ or K.sup.+ may be present as a counter cation.

[0088] It is preferred that Y.sup.1 is selected from O, OH and NH.sub.2. More preferably, Y.sup.1 is selected from O and OH.

[0089] Typically, the integer represented by n.sub.1 is at least 5, preferably at least 10. For example, n.sub.1 may be from 5 to 500, such as from 10 to 300, more preferably from 15 to 100.

[0090] The polymer having a plurality of hemocompatible groups may comprise a repeating unit represented by formula (B-1):

##STR00007##

wherein: [0091] R.sup.C is selected from H and C.sub.1-6 alkyl; [0092] R.sup.D is selected from H, C.sub.1-6 alkyl and C(O)X.sup.2; [0093] each X.sup.2 is independently selected from O.sup.?, OH, OR.sup.3, NH.sub.2, NHR.sup.3 and N(R.sup.3).sub.2; and [0094] each R.sup.3 is independently selected from optionally substituted C.sub.1-6 alkyl, optionally substituted C.sub.6-10 aryl and optionally substituted C.sub.6-10 aryl-C.sub.1-6 alkyl.

[0095] The expression C(O) represents a carbonyl group, i.e. C?O.

[0096] The repeating unit represented by formula (B-1) may be a second repeating unit.

[0097] The repeating unit represented by formula (B-1) above may, for example, be obtained or is obtainable from a monomer of formula (b-1) or a monomer of formula (b-2) as described herein, such as acrylic acid, methacrylic acid, an ester of acrylic acid, an ester of methacrylic acid, an amide of acrylic acid, an amide of methacrylic acid, maleic acid or an ester, amide or anhydride thereof, or maleimide. The ester of methacrylic acid may be 2-hydroxyethyl methacrylate (HEMA) or 2-hydroxypropyl methacrylate. These monomers are commercially available.

[0098] Generally, any layer, such as the polymer layer and/or the surface layer, that has a polymer comprising a repeating unit represented by formula (B-1) will typically comprise a polymer composition. The polymer composition has a distribution of molecules of the polymer, where each molecule of polymer includes a repeating unit (e.g. in varying numbers) as represented by formula (B-1) as described herein.

[0099] The repeating unit represented by formula (B-1) may be further represented by formula (B-2) below.

##STR00008##

[0100] In formula (B-2), n.sub.2 is an integer greater than 0, and each of R.sup.C, R.sup.D and X.sup.2 are as defined herein.

[0101] In formula (B-1) or formula (B-2), it is preferred that R.sup.C is selected from H and C.sub.1-3 alkyl, preferably R.sup.C is selected from H and methyl.

[0102] When R.sup.D is selected from H and C.sub.1-6 alkyl, then preferably R.sup.C is methyl.

[0103] When R.sup.D is C(O)X.sup.2, then preferably R.sup.C is hydrogen.

[0104] In formula (B-1) or formula (B-2), it is preferred that R.sup.D is selected from H, C.sub.1-3 alkyl and C(O)X.sup.2; preferably R.sup.D is selected from H, methyl and C(O)X.sup.2. Even more preferably, R.sup.D is hydrogen or C(O)X.sup.2. R.sup.D may be hydrogen. R.sup.D may be C(O)X.sup.2.

[0105] When R.sup.D is C(O)X.sup.2, then the repeating unit represented by formula (B-1) or formula (B-2) contains two groups represented by X.sup.2. Each X.sup.2 may be the same or different. It is preferred that each X.sup.2 is the same.

[0106] Generally, in formula (B-1) or formula (B-2), when X.sup.2 is OR.sup.3, NHR.sup.3 or N(R.sup.3).sub.2, then each R.sup.3 is independently selected from optionally substituted C.sub.1-6 alkyl, optionally substituted C.sub.6-10 aryl and optionally substituted C.sub.6-10 aryl-C.sub.1-6 alkyl. Thus, each R.sup.3 may be independently selected from unsubstituted C.sub.1-6 alkyl, substituted C.sub.1-6 alkyl, unsubstituted C.sub.6-10 aryl, substituted C.sub.6-10 aryl, unsubstituted C.sub.6-10 aryl-C.sub.1-6 alkyl and substituted C.sub.6-10 aryl-C.sub.1-6 alkyl.

[0107] The C.sub.1-6 alkyl, C.sub.6-10 aryl and the C.sub.6-10 aryl-C.sub.1-6 alkyl may each independently be substituted with one or more substituents selected from hydroxy, C.sub.1-6 alkoxy and halo.

[0108] It is preferred that each R.sup.3 is independently selected from hydroxy-substituted substituted C.sub.2-6 alkyl, unsubstituted C.sub.1-6 alkyl, optionally substituted C.sub.6 aryl and optionally substituted C.sub.6 aryl-C.sub.1-6 alkyl. More preferably, R.sup.3 is independently selected from hydroxy-substituted substituted C.sub.2-6 alkyl and unsubstituted C.sub.1-6 alkyl, such as methyl or ethyl.

[0109] When X.sup.2 is N(R.sup.3).sub.2, then each R.sup.3 may be the same or different. It is preferred that each R.sup.3 is the same.

[0110] The repeating unit represented by formula (B-1) or formula (B-2) may be anionic, such as when X.sup.2 is O. When the polymer chain is anionic, then Na.sup.+ or K.sup.+ may be present as a counter cation.

[0111] In formula (B-1) or formula (B-2), it is preferred that X.sup.2 is selected from O, OH, OR.sup.3, NH.sub.2 and NHR.sup.3. More preferably, X.sup.2 is selected from O, OH, OR.sup.3 and NH.sub.2. X.sup.2 may selected from O, OH and OR.sup.3. Alternatively, X.sup.2 is selected from O, OH and NH.sub.2. Even more preferably, X.sup.2 is selected from O and OH.

[0112] Typically, the integer represented by n.sub.2 is at least 5, preferably at least 10. For example, n.sub.2 may be from 5 to 500, such as from 10 to 300, more preferably from 15 to 100.

[0113] When the polymer having a plurality of hemocompatible groups comprises a repeating unit (e.g. a first repeating unit) represented by formula (A-1) and a repeating unit (e.g. a second repeating unit) represented by formula (B-1), then preferably (a) and/or (b) applies. [0114] (a) R.sup.A is different (e.g. is a different group) to R.sup.D. [0115] (b) R.sup.B is different (e.g. is a different group) to R.sup.C.

[0116] For example, when R.sup.A is R.sup.A is selected from H and C.sub.1-6 alkyl, then R.sup.D may be C(O)X.sup.2.

[0117] In general, it is preferred that when R.sup.B is H, then R.sup.C is C.sub.1-6 alkyl, preferably C.sub.1-3 alkyl, more preferably methyl.

[0118] Typically, the polymer having a plurality of hemocompatible groups is a copolymer.

[0119] The polymer having a plurality of hemocompatible groups comprises, or consists essentially of, (i) a first repeating unit represented by formula (A-1) and (ii) a second repeating unit represented by formula (B-1). Thus, the polymer is a copolymer comprising, or consisting essentially of, (i) a first repeating unit represented by formula (A-1) and (ii) a second repeating unit represented by formula (B-1).

[0120] When the polymer having a plurality of hemocompatible groups is a copolymer, then the copolymer may be an alternating copolymer, a random copolymer or a block copolymer. The copolymer may be an alternating copolymer. The copolymer may be a block copolymer. It is preferred that the copolymer is a random copolymer.

[0121] When the polymer having a plurality of hemocompatible groups is a random copolymer, then the random copolymer comprises, or consists essentially of, (i) a first repeating unit represented by formula (A-1) and (ii) a second repeating unit represented by formula (B-1). The repeating units are randomly distributed in the copolymer (e.g. within the polymer chain of each copolymer molecule).

[0122] When the polymer having a plurality of hemocompatible groups is an alternating random copolymer, then the alternating copolymer comprises, or consists essentially of, (i) a first repeating unit represented by formula (A-1) and (ii) a second repeating unit represented by formula (B-1). For example, the alternating copolymer may comprise, or consist essentially of, alternating first and second repeating units, preferably regularly alternating first and second repeating units (e.g. -(A-1)-(B-1)-(A-1)-(B-1)- . . . ).

[0123] The alternating copolymer may comprise, or consist essentially of, a repeating unit represented by formula (1),

##STR00009##

wherein: [0124] (A-1) is a repeating unit of formula (A-1) as described herein; [0125] (B-1) is a repeating unit of formula (B-1) as described herein; and [0126] n is an integer greater than 0.

[0127] When the polymer having a plurality of hemocompatible groups is a block copolymer, then the block copolymer comprises, or consists essentially of, (i) a first repeating unit represented by formula (A-1) and (ii) a second repeating unit represented by formula (B-1).

[0128] For example, the block copolymer may comprise, or consist essentially of, a repeating unit represented by formula (2),

##STR00010##

wherein: [0129] (A-1) is a repeating unit of formula (A-1) as described herein; [0130] (B-1) is a repeating unit of formula (B-1) as described herein; [0131] n.sub.1 is an integer greater than 0, such as described herein; and [0132] n.sub.2 is an integer greater than 0, such as described herein.

[0133] In an embodiment, the polymer having a plurality of hemocompatible groups does not comprise a repeating unit obtained or obtainable from a monomer of maleic acid or maleimide. In particular, the polymer having a plurality of hemocompatible groups does not comprise a repeating unit as defined in claim 4 and represented by formula (A-1) in the International publication of International patent application no. PCT/EP2020/081383, which is incorporated herein by reference.

[0134] Methods for preparing the polymer having a plurality of hemocompatible groups are known in the art.

[0135] The coating of the invention may further comprise a base layer. The base layer is for contact with, or disposal on, a surface of a medical device.

[0136] The base layer may be disposed on a surface of the medical device. The base layer is typically directly disposed on a surface of the medical device. Thus, the base layer is in direct contact with the surface.

[0137] It is generally preferred that the base layer adheres or bonds, preferably adheres, to a surface of the medical device.

[0138] Typically, the base layer is biocompatible.

[0139] The base layer is typically disposed under the polymer layer or a surface layer.

[0140] A polymer layer or the surface layer is, in general, directly disposed on the base layer. Thus, a polymer layer or the surface layer is typically coated onto the base layer.

[0141] In general, the surface layer comprises, or may consist essentially of, the polymer having a plurality of hemocompatible groups, as described herein.

[0142] When the coating comprises a total of two layers (e.g. only two layers), then the surface layer is the polymer layer. The polymer layer is disposed on the base layer.

[0143] The coating may comprise a plurality of layers disposed on the base layer. One of the plurality of layers is the surface layer. In addition to the surface layer, the plurality of layers may comprise one or more polymer layers, such as described herein, and/or one or more protein layers.

[0144] Generally, each protein layer comprises, or consists essentially of, a protein, such as a protein as described herein.

[0145] It is preferred that the plurality of layers is one or more pairs of layers, wherein each pair of layers consists essentially of a polymer layer and a protein layer. When there is one or more pairs of layers, then it is preferred that a polymer layer from a pair of layers is disposed on the base layer. The surface layer is preferably disposed on a protein layer from a pair of layers.

[0146] For example, the coating may comprise a base layer, a first polymer layer disposed on the base layer, a first protein layer disposed on the first polymer layer, a second polymer layer disposed on the first protein layer, a second protein layer disposed on the second polymer layer, and a surface layer disposed on the second protein layer. In this example, a first pair of layers is the first polymer layer and the first protein layer. A second pair of layers is the second polymer layer and the second protein layer.

[0147] Generally, the base layer comprises, or consists essentially of, a protein, preferably a human protein.

[0148] The protein may be albumin. It is preferred that the albumin is a human albumin, preferably recombinant human albumin.

[0149] Albumin, particularly recombinant human albumin, can mimic the albumin found in the circulatory system of a human. It can adhere to a wide variety of surfaces, especially surfaces of medical devices, by means of a static attraction. It also provides a biopassive surface that can protect against platelet activation.

[0150] In general, a polymer layer (or the surface layer when there is a total of two layers) is conjugated to the base layer.

[0151] When there is a plurality of layers, each polymer layer may be conjugated to an adjacent protein layer. The surface layer may be conjugated to a protein layer.

[0152] When a polymer layer or the surface layer is conjugated to the base layer, then typically the polymer having a plurality of hemocompatible groups (e.g. from the polymer layer or the surface layer) is crosslinked to the protein (e.g. from the base layer).

[0153] Similarly, when a polymer layer is conjugated to an adjacent protein layer, then the polymer having a plurality of hemocompatible groups (e.g. from the polymer layer) is crosslinked to the protein (e.g. from the protein layer).

[0154] A repeating unit represented by formula (A-1) and/or a repeating unit represented by formula (B-1) may be coupled, preferably cross-linked, to the protein, such as to an amino acid of the protein. It is preferred that a repeating unit represented by formula (B-1) is coupled, preferably cross-linked, to the protein.

[0155] Conjugating the layers in this way, typically by forming cross-links between the polymer and the protein, is advantageous because the resulting coating is more robust. The coating can be used in acidic pH conditions without being degraded. The cross-links also improve adherence of the coating to a surface of the substrate, such as when alkaline conditions are used to apply the coating.

[0156] In general, the coating may further comprise an antimicrobial agent. The base layer and/or the surface layer may comprise the antimicrobial agent. The antimicrobial agent may, for example, be mixed with the components of the surface layer or the base layer.

[0157] The antimicrobial agent may comprise silver.

[0158] The coating of the invention is typically disposed on a surface of a medical device. The surface of the medical device may be an inner or an internal surface of the medical device (e.g. the inner surface inside a catheter) or an outer surface of the medical device. The coating may be disposed on a surface of the medical device that will come into with blood or a blood product containing platelets.

[0159] The coating may provide complete or partial coverage of the surface of the medical device.

[0160] In the coating of the invention, the base layer is disposed on a surface of the medical device. The base layer is typically directly disposed on a surface of the medical device. Thus, the base layer is in direct contact with the surface of the medical device.

[0161] The base layer may be directly applied to a surface of the medical device, particularly if it is able to adhere or bond to this surface.

[0162] The invention also provides a medical device. The medical device may be used where it comes into contact with blood or a blood product containing platelets.

[0163] The coating is disposed on a surface of the medical device. Thus, the medical device has a surface coated with the coating of the invention. For convenience, the surface coated with the coating of the invention is referred to as the coated surface. The coated surface is for use in contact with blood or a blood product containing platelets, preferably blood.

[0164] Typically, the coating has a thickness of from 0.005 ?m to 300 ?m, preferably 0.05 ?m to 200 ?m (e.g. 0.1 ?m to 200 ?m), and more preferably 1 ?m to 100 ?m. Generally, the thickness of the coating is selected so that it does not significantly increase the profile of the medical device for use within a patient.

[0165] The medical device may be coated with a coating comprising, or consisting essentially of, the base layer and the surface layer. Alternatively, the medical device may be coated with the coating comprising a plurality of layers disposed on the base layer, as described herein. Thus, a surface of the medical device may be coated with several alternating layers of the protein layer and the polymer layer.

[0166] The medical device can be an implantable medical device or an extracorporeal medical device. The implantable medical device may be a permanently implantable medical device or a temporarily implantable medical device.

[0167] The medical device may be a transient blood contacting device, which may, for example, not be implantable or extracorporeal.

[0168] The medical device may be for in vivo use or for in vitro use. The medical device is preferably for in vivo use.

[0169] The medical device may have a component for in vivo use. It is preferred that at least a surface of the component is coated with the coating of the invention.

[0170] The coated surface of the medical device or the component of the medical device may be inserted into an area of a body, such as an afflicted area of the body. The area of the body may, for example, be an artery or a vein, such as a coronary artery or vein, a carotid artery or vein; a renal artery or vein; an iliac artery or vein; a femoral artery or vein; a popliteal artery or vein; a subclavian artery or vein; an intercranial artery or vein; the aorta; the vena cava; or a peripheral artery or vein.

[0171] Once in place, the coated surface prevents blood coagulation on and around the medical device or the component of the medical device, thereby inhibiting thrombosis, particularly sub-acute device thrombosis.

[0172] The medical device or component thereof may be a needle, catheter, a stent, a graft, a shunt, a dressing, a surgical staple, a guidewire, a cannula, a surgical instrument, an endoscope, an artificial organ or organoid (e.g. insulin secreting device), an implantable monitor or sensor, a defibrillator, a ventricular assist device, a pacemaker (e.g. cardiac pacemaker), an implantable pump (e.g. intra-aortic balloon pump or a ventricular assist pump), a cell reservoir (e.g. for stem cell placement), a prosthetic device (e.g. prosthetic heart valve), an orthopaedic device, an electrostimulation lead or lead tip, an implantable vascular access port, a blood storage bag, blood tubing, a breast implant, a pain management device, a prostate cancer treatment device, a dental implant, a focal epilepsy treatment device, a nerve regeneration conduit, a vena cava filter, a spinal repair device, a spinal cord stimulator, an internal hearing aid, a neuro aneurysm treatment device, a heart valve repair device, a intravitreal drug delivery device, a joint replacement, an ophthalmic implant, a blood oxygenator, a blood filter, a septal defect device, a haemodialysis unit, a hemoperfusion unit, a plasmapheresis unit or an anastomosis device.

[0173] Examples of catheters include a balloon or an inflation catheter, an injection catheter, a central venous catheter, an arterial catheter and an aspiration catheter. It may be preferable that the medical device is a central venous catheter or an aspiration catheter.

[0174] Examples of grafts include vascular grafts, stent grafts or bypass grafts. It may be preferable that the medical device is a bypass graft.

[0175] Examples of stents include a vascular stent, a urethra stent, a bile duct stent, a biliary stent, an oesophageal stent, and a tracheal or bronchial stent.

[0176] In general, it may be preferable that the medical device is selected from a central venous catheter, an aspiration catheter, a bypass graft, a perfusion line, a cardia bypass machine, and extracorporeal oxygenation and heat exchange circuitry.

[0177] The medical device or component thereof may comprise a metal, a polymeric material, glass or a ceramic. Thus, the coating of the invention may be coated onto a surface comprising a metal, a plastic or a ceramic.

[0178] The metal may, for example, be stainless steel, titanium, nickel, tantalum, cobalt, chromium, nickel, molybdenum, manganese, gold, platinum, iridium, silver, tungsten, a titanium alloy (e.g. nitinol), a nickel-chromium alloy (e.g. Inconel), a cobalt chromium alloy (e.g. elgiloy), a ferrous alloy, a palladium alloy, a rhenium alloy or a magnesium alloy.

[0179] The polymeric material may, for example, be cellulose acetate, cellulose nitrate, silicone, polyethylene terephthalate, polyurethane, polyamide, polyester (e.g. Nylon), polyorthoester, polyanhydride, polyether sulfone, polycarbonate, polypropylene, polyethylene or polytetrafluoroethylene.

[0180] The ceramic may, for example, include an oxide, a carbide, or a nitride of a transition metal, such as titanium oxides, hafnium oxides, iridium oxides, chromium oxides, aluminum oxides, and zirconium oxides.

[0181] The invention also provides a method of manufacturing the coating for a medical device. The invention also relates to a coating obtained or obtainable from this method.

[0182] An aspect of the invention is a method of coating a surface of medical device. This method is a method of manufacturing a coating for a medical device that is performed on a surface of the medical device.

[0183] Typically, the method comprises (a) applying a polymer having a plurality of hemocompatible groups as described herein onto a base layer (e.g. onto a base layer on a surface of a medical device). The polymer is applied onto the base layer to form a layer (e.g. a polymer layer or, when the coating comprises only two layers, then a surface layer).

[0184] The method of the invention is advantageous because the polymer having a plurality of hemocompatible groups is pre-formed before it is applied on to a base layer or a protein layer. This avoids the need to functionalise a precursor polymer with the hemocompatible groups in situ, such as when the precursor polymer has been applied onto a protein-containing layer. The formation of the polymer having a plurality of hemocompatible groups in situ when part of a layer restricts the chemical methodology that can be used because it may affect the protein-containing layer. This method permits a wider range of methodologies to be used to prepare the polymer.

[0185] The method of the invention allows a coating to be prepared having multiple layers, which can be built up layer-by-layer according to the required performance and cost. The layers can also be applied to a surface of the medical device without the need for solvents.

[0186] The method may comprise (a1) preparing a base layer as described herein on a surface of a medical device, then (a2) applying a polymer having a plurality of hemocompatible groups as described herein onto the base layer.

[0187] In step (a1) of the method, the base layer may be prepared by applying a base layer, such as by applying a base coating solution, to a surface of the medical device (e.g. to form a coated surface). After applying the base coating solution to the surface of the medical device, the coated surface is dried or is allowed to dry.

[0188] In general, the base coating solution comprises a protein as described herein. The base coating solution may be an aqueous solution, preferably a buffered aqueous solution, comprising the protein.

[0189] The base coating solution may have a pH of <7, such as a pH from about 3.5 to about 6.5, preferably a pH of from about 4.0 to about 6.0.

[0190] The base coating solution may further comprise a stabiliser for the protein.

[0191] In principle, the base coating solution may be applied to a surface of the medical device using conventional techniques.

[0192] For example, the base coating solution can be applied to a surface of the medical device by, for example, dip-coating, spraying, washing, brushing, rollering, wiping, or spin coating. The base coating solution may be poured onto a surface of the medical device or the surface of the medical device may be soaked in or rinsed with the base coating solution. Alternatively, the base coating solution may be pumped through the medical device, such as to coat an inner surface of the medical device.

[0193] It is preferred that a surface of the medical device is soaked in, or rinsed with, the base coating solution.

[0194] The thickness of the base layer may be controlled by altering soaking time, flow rate etc and the number of coating steps. Each coating can be provided on a surface of the medical device as described herein in a series of applications. The number of applications may be selected to provide individual coated layers of suitable thickness, as well as a desired total number of multiple coatings, as desired.

[0195] The base layer is applied to a surface of the medical device before the surface layer.

[0196] Generally, step (a1) produces a base layer on a surface of the medical device.

[0197] Step (a1) may be repeated to produce the base layer. By repeating step (a1), the thickness of the base layer may be increased.

[0198] In step (a2), the polymer having a plurality of hemocompatible groups may be applied onto the base layer by applying a polymer coating dispersion onto the base layer (e.g. to form or provide a coated base layer). After applying the polymer coating dispersion onto the base layer, the coated base layer is dried or is allowed to dry.

[0199] In general, the polymer coating dispersion comprises the polymer having a plurality of hemocompatible groups and a liquid vehicle.

[0200] The polymer coating dispersion may be a solution or a suspension. Thus, the polymer may be dissolved in the liquid vehicle (e.g. when the polymer coating dispersion is a solution) or suspended in the liquid vehicle (e.g. when the polymer coating dispersion is a suspension).

[0201] The liquid vehicle may be water or ethanol. It is preferred that the liquid vehicle is water, preferably deionised water.

[0202] The polymer coating dispersion is preferably buffered.

[0203] The polymer coating dispersion may have a pH of <7, such as a pH from about 3.5 to about 6.5, preferably a pH of from about 4.0 to about 6.0.

[0204] The polymer coating dispersion may be applied onto to the base layer using conventional techniques. For example, the polymer coating dispersion can be applied to a base layer (e.g. on a surface of the medical device) by, for example, dip-coating, spraying, washing, brushing, rollering, wiping, or spin coating. The polymer coating dispersion may be poured onto the base layer or the base layer on the surface of the medical device may be soaked in or rinsed with the polymer coating dispersion. Alternatively, the polymer coating dispersion may be pumped through the medical device, such as to coat the base layer on an inner surface of the medical device.

[0205] It is preferred that a surface of the medical device is soaked in, or rinsed with, the base coating solution.

[0206] The thickness of the polymer layer may be controlled by altering soaking time, flow rate etc and the number of coating steps.

[0207] It may be preferred that the base layer (e.g. on a surface of the medical device) is soaked in or rinsed with the polymer coating dispersion.

[0208] Generally, step (a2) produces a polymer layer on a protein layer, such as the base layer. When the coating comprises only two layers, then the polymer layer is the surface layer.

[0209] Step (a2) may be repeated to produce the polymer layer or the surface layer. By repeating step (a2), the thickness of the polymer layer or the surface layer may be increased.

[0210] When the coating comprises only two layers, then there is a single protein layer, which is the base layer, and a single polymer layer, which is the surface layer.

[0211] The coating may comprise a plurality of protein layers (e.g. including the base layer) and/or a plurality of polymer layers (e.g. including the surface layer).

[0212] After steps (a1) and (a2), the method may further comprise (a3) applying a protein layer as described herein to a polymer layer, then (a4) applying a polymer having a plurality of hemocompatible groups as described herein to the protein layer.

[0213] In step (a3) of the method, the protein layer may be prepared by applying the base coating solution as described herein to a polymer layer (e.g. to form a coated polymer layer surface). After applying the base coating solution to the polymer layer, the coated polymer layer surface is dried or is allowed to dry.

[0214] Step (a3) may be performed in the same way as step (a1). Thus, the base coating solution may be applied in the same way to the polymer layer as the base coating solution is applied to the surface of the medical device.

[0215] Generally, step (a3) produces a protein layer on the polymer layer.

[0216] Step (a3) may be repeated to produce the protein layer (e.g. before performing step (a4)). By repeating step (a3), the thickness of the protein layer may be increased.

[0217] In step (a4), the polymer having a plurality of hemocompatible groups may be applied onto the protein layer by applying a polymer coating dispersion as described herein onto the protein layer (e.g. to form or provide a coated protein layer). After applying the polymer coating dispersion onto the protein layer, the coated protein layer is dried or is allowed to dry.

[0218] Step (a4) may be performed in the same way as step (a2). Thus, the polymer coating dispersion may be applied in the same way to the protein layer as the polymer coating dispersion is applied to the base layer.

[0219] Generally, step (a4) produces a polymer layer on the protein layer.

[0220] Step (a4) may be repeated to produce the polymer layer. By repeating step (a4), the thickness of the polymer layer may be increased.

[0221] Both steps (a3) and (a4) may be performed once or repeated. It may be preferable to perform both steps (a3) and (a4) one, two or three times.

[0222] By performing steps (a3) and (a4), a coating having a plurality of layers may be manufactured, where the plurality of layers is one or more pairs of layers, wherein each pair of layers consists essentially of a polymer layer and a protein layer.

[0223] When step (a4) is performed for the final time, then the resulting polymer layer is the surface layer.

[0224] In general, the method of the invention comprises (b) coupling the polymer having a plurality of hemocompatible groups to the protein using a coupling agent. The coupling the polymer to the protein is preferably cross-linking the polymer to the protein.

[0225] The polymer may be cross-linked to the protein by forming amide bonds between the polymer and the protein. For example, the carboxylic acid, ester or amide in the repeating unit of formula (B-1) may form an amide bond with amino acids in the protein.

[0226] Typically, the coupling agent is a carbodiimide coupling agent. Carbodiimide coupling agents are known in the art.

[0227] The carbodiimide coupling agent may be selected from N,N-dicyclohexylcarbodiimide (DCC), N,N-diisopropylcarbodiimide (DIC) and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC). It is preferred that the carbodiimide coupling agent is EDC.

[0228] In step (b) of the method, the coupling the polymer having a plurality of hemocompatible groups to the protein using a coupling agent may be by applying a coupling agent solution to a coating comprising a protein layer as described herein and a polymer layer as described herein. The protein layer comprises the protein and the polymer layer comprises the polymer having a plurality of hemocompatible groups.

[0229] The coupling agent may be applied to the surface layer of the coating.

[0230] Typically, the polymer layer is the surface layer.

[0231] The protein layer may or may not be the base layer.

[0232] In general, the coupling agent solution comprises a coupling agent as described herein, such as a carbodiimide coupling agent.

[0233] The coupling agent solution may be an aqueous solution, preferably a buffered aqueous solution.

[0234] In principle, the coupling agent solution may be applied to the coating using conventional techniques.

[0235] The coupling agent solution may be applied onto to the base layer using conventional techniques. For example, the coupling agent solution can be applied to a polymer layer (e.g. on a surface of the medical device), such as the surface layer, by dip-coating, spraying, washing, brushing, rollering, wiping, or spin coating. The coupling agent solution may be poured onto the polymer layer (e.g. the surface layer) on the surface of the medical device may be soaked in or rinsed with the coupling agent solution. Alternatively, the coupling agent solution may be pumped through the medical device, such as to coat the polymer layer (e.g. the surface layer) on an inner surface of the medical device.

[0236] It is preferred that the coating is soaked in, or rinsed with, the coupling agent solution.

[0237] Generally, step (b) is performed to produce a crosslinked coating on a surface of the medical device. Before applying the coupling agent, the coating on the surface of the medical device is typically non-crosslinking (i.e. a polymer layer, such as the surface layer, is not conjugated to a protein layer, such as the base layer).

[0238] Step (b) may include repeatedly applying a coupling agent solution to the coating comprising the protein layer and the polymer layer. This may be to ensure sufficient cross-linking within the coating.

[0239] In the method of the invention, step (b) may be performed directly after step (a), such as directly after steps (a1) and (a2). Additionally or alternatively, step (b) may be performed after steps (a3) and (a4).

[0240] Step (b) may be performed after each repetition of steps (a3) and (a4). Alternatively, step (b) may be performed after all repetitions of steps (a3) and (a4) have been performed.

[0241] Typically, step (b) is the final step (e.g. the final step involving applying a solution) of the method.

[0242] Any remaining coupling agent solution can be removed, such as by rinsing with water.

[0243] In general, it is preferred that step (b) is only performed after the surface layer has been applied (e.g. the surface layer of a non-crosslinked coating). Thus, a single coupling step is performed once the layers have been applied to a surface of the medical device.

[0244] Another general feature of the method of the invention is that (a) may include preparing a polymer having a plurality of hemocompatible groups.

[0245] The polymer having a plurality of hemocompatible groups may be prepared by (i) polymerising a monomer of formula (a-1),

##STR00011##

wherein R.sup.A, R.sup.B and X.sup.1 are as described herein in relation to the repeating unit of formula (A-1).

[0246] The monomer of formula (a-1) may preferably be 2-acrylamido-2-methyl-1-propane sulfonic acid (AMPS), 3-(acryloyloxy)propane-1-sulfonic acid or 3-(methacryloyloxy)-propane-1-sulfonic acid. It is preferred that the monomer of formula (a-1) is 2-acrylamido-2-methyl-1-propane sulfonic acid (AMPS).

[0247] It is preferred that the polymer having a plurality of hemocompatible groups is prepared by (i) copolymerising a monomer of formula (a-1) as described herein with a monomer of formula (b-1) or (b-2),

##STR00012##

wherein R.sup.C, R.sup.D and X.sup.2 are as described herein in relation to the repeating unit of formula (B-1); and X.sup.3 is selected from O, NH and NR.sup.3, wherein is R.sup.3 as defined herein. It is preferred that X.sup.3 is selected from O and NH, more preferably X.sup.3 is O.

[0248] The monomer of formula (b-1) may preferably be acrylic acid, methacrylic acid, an ester of acrylic acid, an ester of methacrylic acid, an amide of acrylic acid, an amide of methacrylic acid, maleic acid or an ester or an amide thereof. The ester of methacrylic acid may be 2-hydroxyethyl methacrylate (HEMA) or 2-hydroxypropyl methacrylate. The monomer of formula (b-2) may be maleic anhydride or maleimide.

[0249] It is preferred that the polymer having a plurality of hemocompatible groups is prepared by (i) copolymerising a monomer of formula (a-1) as described herein with a monomer of formula (b-1).

[0250] Typically, the copolymerisation is a free radical copolymerisation.

[0251] The copolymerisation, particularly the free radical copolymerisation, may be performed using methods known in the art.

[0252] In (i), the copolymerising a monomer of formula (a-1) with a monomer of formula (b-1) or formula (b-2) uses a copolymerisation catalyst.

[0253] When the copolymerisation is a free radical copolymerisation, then the copolymerisation catalyst may be a free radical initiator.

[0254] The free radical initiator may be any free radical initiator suitable for copolymerisation of the monomers. It is preferred that the free radical initiator is non-toxic to humans, preferably biocompatible.

[0255] The free radical initiator may, for example, be a thermal polymerisation initiator, a photopolymerisation initiator, a photocationic initiator or a photoanionic initiator.

[0256] The thermal polymerisation initiator may be an organic peroxide, an azo compound or a persulfate.

[0257] When the thermal polymerisation initiator is an organic peroxide, then the organic peroxide may be selected from tert-butylhydroperoxide (TBHP), cumene hydroperoxide, di-tert-butylhydroperoxide, dicumyl peroxide and benzoyl peroxide (BPO).

[0258] When the thermal polymerisation initiator is an azo compound, then the azo compound may be selected from azoisobutylnitrile (AIBN) and 2,2-azobis[2-(2-imidazolin-2-yl)propane]dihydrochloride. It is preferred that the thermal polymerisation initiator is azoisobutylnitrile (AIBN).

[0259] When the thermal polymerisation initiator is a persulfate, then the persulfate may be selected from ammonium persulfate (also known as ammonium peroxydisulfate), sodium persulfate and potassium persulfate. It is preferred that the persulfate is ammonium persulfate.

[0260] Examples of the photopolymerisation initiator include (+)-camphorquinone, acetophenone, 4-hydroxyacetophenone, 3-hydroxyacetophenone, benzophenone, 3-methylbenzophenone, 2-methylbenzophenone, 3,4-dimethylbenzophenone, 3-hydroxybenzophenone, 4-hydroxyphenone, 4,4-dihydroxybenzophenone, 4-benzoylbenzoic acid, 2-benzoylbenzoic acid, methyl 2-benzoylbenzoate, 4,4-carbonyldiphthalic anhydride, 4-(dimethylamino)benzophenone, 4,4-bis(dimethylamino)benzophenone, 4,4-bis(diethylamino)benzophenone, 4,4-dichlorobenzophenone, 4-phenylbenzophenone, 1,4-dibenzoylbenzene, 4-(p-tolylthio)benzophenone, dibenzosuberenone, benzil, p-anisil, methyl benzoylformate, 9,10-phenanthrenequinone, 2-benzoyl-2-propanol, 2-hydroxy-4-(2-hydroxyethoxy)-2-methylpropiophenone, 1-benzoylcyclohexanol, benzoin, anisoin, benzoin methyl ether, benzoin ethyl ether, benzoin isopropyl ether, benzoin isobutyl ether, 2,2-diethoxyacetophenone, benzil dimethylketal, 2-methyl-4-(methylthio)-2-morpholinopropiophenone, 2-benzyl-2-(dimethylamino)-4-morpholiniobutyrophenone, 2-isonitrosopropiophenone, 9,10-phenantrenequinone, 2-ethylanthraquinone, sodium antraquinone-2-sulfonate monohydrate, 2-chlorothioxanthone, 1-chloro-4-propoxythioxanthone, 2-isopropylthioxanthone, 2,4-diethylthioxanthen-9-one and 2,7-dimethoxythioxanthone.

[0261] Examples of the photocationic initiator include diphenyliodonium trifluoromethanesulfonate, diphenyliodonium hexafluoroarsenate, diphenyliodonium hexafluorophosphate, triphenylsulfonium bromide, triphenylsulfonium tetrafluoroborate, triphenylsulfonium nonaflate, tri-p-tolylsulfonium triflate, tri-p-tolylsulfonium hexafluorophosphate, 4-nitrobenzenediazonium tetrafluoroborate, 2-(4-methoxyphenyl)-4,6-bis(trichloromethyl)-1,3,5-triazine, 2-(1,3-benzodioxol-5-yl)-4,6-bis(trichloromethyl)-1,3,5-triazine, 2-(4-methoxystyryl)-4,6-bis(trichloromethyl)-1,3,5-triazine, 2-(3,4-dimethoxystyryl)-4,6-bis(trichloromethyl)-1,3,5-triazine and 2-[2-(furan-2-yl)vinyl]-4,6-bis(trichloromethyl)-1,3,5-triazine.

[0262] Examples of the photoanionic initiator include acetophenone O-benzoyloxime, 2-nitrobenzyl cyclohexylcarbamate, 1,2-bis(4-methoxyphenyl)-2-oxoethyl cyclohexylcarbamate, 1-(2-formylbenzoyl)piperidine and nifedipine.

[0263] In general, it is preferred that the free radical initiator is a thermal polymerisation initiator, such as an azo compound or a persulfate.

[0264] The invention also provides a kit for coating a medical device. The kit comprises: (a) a polymer having a plurality of hemocompatible groups, as described herein; (b) a base coating solution, as described herein; and (c) a coupling agent.

[0265] The kit preferably comprises (a) a polymer coating dispersion, as described herein, which comprises the polymer having a plurality of hemocompatible groups.

[0266] Additionally or alternatively, the kit preferably comprises (c) a coupling agent solution as described herein, which comprises the coupling agent.

[0267] In general, the kit may comprise (d) instructions for preparing a coating on a surface of a medical device.

[0268] Also provided by the invention are uses of the coating and the medical device.

[0269] The coating or a medical device comprising the coating can be used in the treatment of the human or animal body by surgery or therapy and/or in a diagnostic method practised on the human or animal body.

[0270] The diagnostic method practised on the human or animal body is typically an in vivo diagnostic method.

[0271] The diagnostic method or the treatment by surgery or therapy typically involves the coating, or the medical device comprising the coating, coming into contact with blood.

[0272] The coating or a medical device comprising the coating is for use in reducing or preventing the clotting of blood, preferably reducing or preventing the clotting of blood on the coated surface of the medical device.

[0273] The invention also provides a method of reducing or preventing the clotting of blood. The method comprises contacting a medical device of the invention with blood. The medical device is used in its normal manner, except that the presence of coating inhibits or prevents the formation of blood clots, particularly on the coated surface of the medical device.

[0274] One aspect of the method is an in vitro method. For example, the method may be an in vitro method of reducing or preventing the clotting of blood when processing the blood. The blood may be processed to produce processed blood or a blood product, such as a blood product for storage.

[0275] The method may comprise contacting the medical device with blood, wherein the blood has been removed from a human or animal body. The blood may be contacted with the medical device to process the blood.

[0276] The method may not include a step of administering the processed blood or the blood product to the human or animal body. The blood product or processed blood may not be returned to the human or animal body, preferably the human or animal body from which the blood was removed.

[0277] A further aspect of the method is a diagnostic method, particularly an in vitro and/or ex vivo diagnostic method.

[0278] The method may relate to a method of reducing or preventing the clotting of blood in a diagnostic procedure. The method may comprise contacting the medical device with blood to obtain diagnostic information. The medical device may be contacted with pre-obtained or pre-delivered blood.

[0279] The diagnostic procedure is not carried out on the human or animal body or the diagnostic procedure is carried out on a non-living (e.g. dead) human or animal body. Thus, the diagnostic method may not include a step of removing blood from a human or animal body, particularly a living human or animal body.

EXAMPLES

[0280] The invention will now be illustrated by the following non-limiting examples.

Example 1

Preparation of AMPS-MAA Copolymer

[0281] A round bottom flask equipped with a magnetic stirrer bar was charged with 2-acrylamido-2-methylpropane sulfonic acid (AMPS), deionised (DI) water, methacrylic acid (MAA) and azoisobutylnitrile (AIBN). The amount of each ingredient that was used is shown in Table 1 below.

[0282] The solution was stirred vigorously to dissolve the AMPS. A condenser (with bubbler) was fitted and the mixture sparged with nitrogen for 30 minutes. The nitrogen sparge was converted to a nitrogen sweep and the mixture heated to 70? C. for 24 hours. As the polymerisation proceeded the mixture took on a slightly hazy appearance. The mixture was cooled and the slightly hazy, moderately viscous solution was discharged.

TABLE-US-00001 TABLE 1 Batch AMPS (g) MAA (g) AIBN (g) DI H.sub.2O % No. [mmol] [mmol] [mmol] (g) Polymer 1 8.84 3.67 0.164 112.5 g 10% [43] [43] [1] 2 10.62 1.89 0.164 112.5 g 10% [51] [22] [1] 3 21.19 8.69 0.328 120.0 g 20% [103] [103] [2] 4 25.49 4.51 0.328 120.0 g 20% [123] [52] [2]

Analytical Data

[0283] The characterising data for each batch is shown in Table 2 below. In the NMR data, the intensity of the peaks is shown in bold.

TABLE-US-00002 TABLE 2 Batch .sup.1H NMR (298 K, D.sub.2O) AMPS:MAA IR (Water Blank) No. ?/ppm ratio cm.sup.?1 1 0.96 (br, s) CH.sub.3, 6, 1.41 (br, 0.97:1.00 1709 (Carboxylic C?O), s) CH.sub.3, 3, 2.00 (br m) CH.sub.2 1, 1647 (Amide C?O), 2, 6, 3.24 (br, m) CH.sub.2 4 1451 (Amide CN) 2 0.94 (br, s) CH.sub.3, 6, 1.41 (br, 2.31:1.00 1712 (Carboxylic C?O), s) CH.sub.3, 3, 2.01 (br m) CH.sub.2 1, 1633 (Amide C?O), 2, 6, 3.24 (br, m) CH.sub.2 4 1451 (Amide CN) 3 0.97 (br, s) CH.sub.3, 6, 1.43 (br, 1.13:1.00 1709 (Carboxylic C?O), s) CH.sub.3, 3, 1.99 (br m) CH.sub.2 1, 1637 (Amide C?O), 2, 6, 3.30 (br, m) CH.sub.2 4 1451 (Amide CN) 4 0.97 (br, s) CH.sub.3, 6, 1.42 (br, 2.36:1.00 1718 (Carboxylic C?O), s) CH.sub.3, 3, 2.01 (br m) CH.sub.2 1, 1636 (Amide C?O), 2, 6, 3.29 (br, m) CH.sub.2 4 1454 (Amide CN)

Recombinant Human Albumin Coating

[0284] A solution of recombinant human albumin (Albumedix?) was prepared in a distilled water in a DI water buffer at a pH of 4.0 to 4.5 (via HCl & NaOH titration) to provide a concentration of the albumin in solution of approximately 0.05 g/2000 mL.

[0285] Samples of PVC tubing were washed with, or suspended in, the albumin solution at room temperature for 20 minutes to produce samples coated with human albumin.

[0286] All of the albumin coated samples were subsequently washed with, or soaked in, DI water for 10 minutes and then dried.

Coating with AMPS-MAA Copolymer

[0287] A solution of the AMPS-MAA copolymer from Batch No. 4 above was prepared using a DI water buffer at a pH of 4.0 to 4.5 (via HCl & NaOH titration) at a concentration of 0.2 mL per 1000 mL.

[0288] The samples coated with human albumin were washed with, or soaked in, the solution of the AMPS-MAA copolymer for 20 minutes.

[0289] After applying the AMPS-MAA copolymer coating, the coated samples were subsequently washed with, or soaked in, DI water for 10 minutes and then dried.

Crosslinking the Coatings

[0290] A solution of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) was prepared in a DI water buffer at a pH of 5.0 to 5.5 (via HCl & NaOH titration) to provide a concentration of 75 mg of EDC per 1000 mL.

[0291] The coated samples were washed for 20 minutes in the EDC solution. After washing in the EDC solution, the samples were subsequently washed with DI water for 30 minutes and then dried.

Coated Samples

[0292] Two sets of samples were prepared using the albumin and copolymer coating processes described above. A first set of samples had a single coating of human albumin and the AMPS-MAA copolymer, which are prepared as described above.

[0293] A second set of samples were prepared having a triple coating of each of the albumin and copolymer layers, where the layers were applied in an alternating arrangement. For the second set of samples, the process above of applying the albumin coating and then the copolymer coating was followed, and repeated for a total of three times.

[0294] Both the first set and the second set of samples were treated with toluidine blue. Toluidine blue has a high affinity for acidic groups, such as any sulfonic acid groups present in the coating. After the treatment, the coated samples showed a pink colour, which confirmed the presence of the sulfonic acid groups.