TREATMENT OF TOOTH DECAY USING A CHEWING GUM COMPOSITION COMPRISING CANNABINOIDS

Abstract

The invention is directed to a food product for use in the prevention, inhibition or treatment of tooth decay and other dental conditions, diseases and disorders, wherein said food product is a chewing gum comprising at least one cannabinoid.

Claims

1. A method of preventing, inhibiting or treating tooth decay and/or dental related condition, disease or disorder in a subject in need thereof; said method comprises administering to said subject a food product comprising at least one cannabinoid, wherein said food product is a chewing gum product; for use in the prevention, inhibition or treatment of tooth decay.

2. (canceled)

3. A method according to claim 1, wherein said at least one cannabinoid compound is an endo-cannabinoid compound or derivative or precursor,

4. A a method according to claim 1, wherein said at least one endo cannabinoid derived compound is select from RAS (arachidonoyl serine), 2AG (2-arachidonoyl glycerol), AEA (arachidonoyl ethanol amide), OEA (oleoyl ethanolamide), OG (oleoyl glycine), OA (oleoyl alanine), FIV-308, PIHA (pahnitoyl ethanolamide) AraG (Arachidonoyl glycine), PG (Palmitoyl glycine), AraA (Arachidonoyl alanine), PA (Palmitoyl alanine), PS (Palmitoyl serine), OS (Oleoyl serine), 2-arachidonoyl glyceryl ether, 2-oleoyl glyceryl ether , 2-palmitoyl glyceryl ether and any derivative or combinations thereof.

5. A method according to claim 1, wherein said food product comprises synthetic, plant derived or derivatives of cannabinoid or endo cannabinoids.

6. A method according to claim 1, wherein said food product further comprises at least one ingredient selected from a breath freshener, a flavoring agent, a colorant, an anti-microbial agent, an anti-inflammatory agent, teeth brightening agent, teeth strengthening agent, agent capable of dilating the upper airways and any combinations thereof.

7. A method according to claim 1, wherein said dental related condition disease or disorder is selected from tooth decay, dental plaque formation, supra-gingivae diseases, halitosis and any combinations thereof.

8. (canceled)

9. (canceled)

Description

DETAILED DESCRIPTION OF THE PRESENT INVENTION

[0033] In the following detailed description, numerous specific details are set forth in order to provide a thorough understanding of the invention. However, it will be understood by those skilled in the art that the present invention may be practiced without these specific details. In other instances, well-known methods, procedures, and components have not been described in detail so as not to obscure the present invention.

[0034] The Anti-Biofilm/Antimicrobial Effects of CBD-Chewing Gum of the Invention

[0035] A chewing gum is immersed in a 20 ml artificial saliva fluid for 15 min. while being in a pestle and mortar at a rate of one crush for 1 sec.

[0036] Artificial saliva samples are taken every 3 min, while supplementing the media with new fresh artificial saliva. The samples are placed with different oral bacteria as Streptococcus mutant, Streptococcus sobrinus (highly cariogenic bacteria) and incubated at 37C for a period of 24 hours.

[0037] The antimicrobial effect is examined by OD/CFU/qPCR. The anti-biofilm effect will be tested while incubating the artificial saliva with 2% sucrose. The biofilm is examined by CV/MTT and CLSM. The chewing gums is tested against placebo chewing gums. The effect of the chewing gum to affect microbial growth and biofilm is tested also when the strip of the chewing gum is placed in a test tube with and without sucrose.

[0038] Comparison of a Chewing Gum of the Invention with a Sugar-Less Chewing Gum CBD—chewing gum of the invention is compared to sugarless chewing gum of Orbit, Wrigley.

[0039] The chewing gums are placed in a media of artificial saliva and pressure at a rate of 1 sec applied for a period of 15 min-mimicking chewing conditions in the mouth.

[0040] Supernatant fluid is taken every 2 min and placed in a suspension of cariogenic bacteria as streptococcus mutans—the effect on the growth of the bacteria is measured for 24 hours.

[0041] The chewing process above is conducted on hydroxyapatite tables (mimicking the tooth) covered with cariogenic bacteria biofilm (mimicking the dental plaque) and the amount of bacteria on the hydroxyapatite and in the artificial saliva is measured every 2 min

[0042] The results of the CFU/OD/MTT/SEM/CLSM are compared between the CBD-chewing gum of the invention and the reference chewing gum and placebo chewing gum.