FLAMMLINA FILIFORMIS "JIN JIN NO.1" WITH FRUITING BODY OF GOLDEN CAP AND WHITE STIPE

Abstract

Disclosed is a Flammlina filiformis with a fruiting body of a golden cap and a white stipe. The Flammlina filiformis has a strain number of Jin Jin No. 1 and has been preserved on Jun. 16, 2022 at the China General Microbiological Culture Collection Center with a registration number of CGMCC No. 40222. Colors of the cap and the stipe of the Flammlina filiformis Jin Jin No. 1 have obvious differences. Compared with a parent white Flammlina filiformis strain F0012, the Flammlina filiformis Jin Jin No. 1 has an equivalent average yield, an initial harvest period 1-2 days late, and a golden cap.

Claims

1. A Flammlina filiformis, comprising a strain number of Jin Jin No. 1, and a preservation number of CGMCC No. 40222.

2. A fruiting body, a mycelium, or spores of a Flammlina filiformis according to claim 1.

3. Application of a Flammlina filiformis according to claim 1 in the preparation of a Flammlina filiformis fruiting body and/or a Flammlina filiformis mycelium and/or Flammlina filiformis spores.

4. Application of a Flammlina filiformis according to claim 1 in Flammlina filiformis breeding.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0021] FIG. 1 is a diagram showing a breeding process of a strain Jin Jin No. 1 according to the present disclosure.

[0022] FIG. 2 is a photograph of a yellow parent Flammlina filiformis strain F0027 of the strain Jin Jin No. 1 according to the present disclosure. F0027 has a fruiting body with a golden cap and a golden stipe.

[0023] FIG. 3 is a photograph of a white parent Flammlina filiformis strain F0012 of the strain Jin Jin No. 1 according to the present disclosure. F0012 has a fruiting body with a white cap and a white stipe.

[0024] FIG. 4 is a photograph of the strain Jin Jin No. 1 according to the present disclosure. Jin Jin No. 1 has a fruiting body with a golden cap and a white stipe.

[0025] FIG. 5 is a photograph showing the fruiting production of the strain Jin Jin No. 1 according to the present disclosure. Jin Jin No. 1 has a fruiting body with a golden cap and a white stipe, and there is no browning at a base during growth.

PRESERVATION INSTRUCTIONS

[0026] Latin Name: Flammlina filiformis [0027] Strain Number: Jin Jin No. 1 [0028] Preservation Institution: China General Microbiological Culture Collection Center [0029] Abbreviation of Preservation Institution: CGMCC [0030] Address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing [0031] Preservation Date: Jun. 16, 2022 [0032] Registration Number in Preservation Institution: CGMCC No. 40222

DETAILED DESCRIPTION

[0033] The present disclosure will be further described in detail with reference to specific embodiments, and the examples are given only to illustrate the present disclosure, but not to limit the scope of the present disclosure. The examples provided below can be used as a guide for further improvement by those of ordinary skill in the art, and are not intended to limit the present disclosure in any way.

[0034] The experimental methods in the following examples, unless otherwise specified, are conventional methods, which are carried out according to the techniques or conditions described in the literature in the art or according to the product specifications. The materials and reagents used in the following examples can be obtained commercially unless otherwise specified.

[0035] Unless otherwise specified, the quantitative experiments in the following examples are all repeated three times, and the results are averaged.

Example 1. Breeding Process and Phenotype of Flammlina filiformis Strain Jin Jin No. 1

[0036] 1. Breeding Process

[0037] In recent years, based on resource collection and genetic analysis of Flammlina filiformis, an Edible Fungi Team of Shanxi Agricultural University had bred a new Flammlina filiformis strain Jin Jin No. 1. The breeding process was shown in FIG. 1. The starting strains used were as follows.

[0038] A yellow Flammlina filiformis strain F0027 (derived from isolated strains of fruiting body tissues of a strain named San Ming No. 1, preserved in the Edible Fungi Center of Shanxi Agricultural University, seen FIG. 2 for the photograph), was the most widely main cultivated variety of yellow Flammlina filiformis in China for more than 20 years, and was also the most widely used yellow Flammlina filiformis variety in genetic background. The fruiting body of the yellow Flammlina filiformis strain had a golden cap and a golden stipe, but it would appear obvious browning in a later growth stage.

[0039] A white Flammlina filiformis strain F0012 (derived from isolated strains of fruiting body tissues of variety FV088 introduced from Japan by factory enterprises in Shanxi Province, and now stored in the Edible Fungi Center of Shanxi Agricultural University, seen FIG. 3 for the photograph), was the main cultivated variety of white Flammlina filiformis introduced from Japan by factory enterprises, had higher yield and better quality. The fruiting body of the white Flammlina filiformis strain had a white cap and a white stipe.

[0040] The breeding was mainly based on the method recorded in the authorized national invention patent ZL201710355494.9 (A METHOD FOR BREEDING PALE YELLOW FLAMMLINA FILIFORMIS, with an authorization announcement number of 107142257B and an authorization announcement date of Oct. 9, 2020).

[0041] Based on the resource collection and genetic analysis of Flammlina filiformis, the new strain Jin Jin No. 1 (an original strain numbered F8-13-12) was a fertile heterokaryotic strain F8-33-8 with a white strain cytoplasm and a heterozygous nucleus by taking main cultivated strains of yellow Flammlina filiformis F0027 (an original variety named San Ming No. 1) and white Flammlina filiformis F0012 (a variety imported from Japan by factory enterprises) as starting parent strains and after the collection of Flammlina filiformis, isolation of single spore, acquisition of monokaryon hyphae, determination of polarity of monokaryon hyphae and population construction, single-single strain pairing hybridization, bidirectional nuclear migration and acquisition of homonuclear heterogeneous strains, double-single hybridization and cytoplasmic purification, biological difference detection and fertility verification of homonuclear heterogenous strains. Then, through a fruiting experiment, an F8 progeny mononuclear strain population (80-100 strains) was established, and the progeny F8 mononuclear strain population was atypically back-crossed with a mononuclear strain F13 (a progeny mononuclear strain from F0012 strain). Then, through biological characteristics analysis and fruiting screening test, a new fertile heterokaryotic strain Jin Jin No. 1 (the original strain numbered F8-13-12) was preferably selected from the heterokaryotic strain population obtained on a side of F13.

[0042] 2. Identification of the Strain Jin Jin No. 1

[0043] The hyphae morphology of Jin Jin No. 1 strain was semi-aerial type, the fruiting body cap had a golden cap with an oblate longitudinal section and a moderate inward curling degree, and the cap was uniform in size and difficult to open. The whole stipe was white with high color uniformity, and there was no browning at a base during growth. Jin Jin No. 1 had excellent quality and was suitable for fresh sale and canned processing. Compared with common varieties of Flammlina filiformis, Jin Jin No. 1 had strong resistance to bacterial diseases. The photograph of the Jinjin No. 1 strain was shown in FIG. 4, and the photographs of the fruiting production was shown in FIG. 5.

[0044] Preservation of the strain Jin Jin No. 1

[0045] Flammlina filiformis Jin Jin No. 1, had been preserved on Jun. 16, 2022 in the China General Microbiological Culture Collection Center (CGMCC, at No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing; Institute of Microbiology, Chinese Academy of Sciences; post code: 100101) with a preservation number of CGMCC No. 40222. The Flammlina filiformis Jin Jin No. 1 was abbreviated as Jin Jin No. 1.

[0046] Cultivation Mode

[0047] The Jin Jin No. 1 strain was suitable for factory production of Flammlina filiformis in bottle cultivation and bag cultivation in China, but it had not been found to be different from the biological requirements of the current production conditions.

[0048] The optional formula of solid materials for Jin Jin No. 1 planting substrate included (in parts by mass): 35 parts of rice bran, 35 parts of corncob, 8 parts of brans, 4.9 parts of soybean hulls, 4.9 parts of brewer's spent grain, 4.9 parts of cottonseed hulls, 4.6 parts of beet pulp and 2.1 parts of hull ash.

[0049] A moisture content, a pH value and a sugar content of the substrate were detected during the cultivation. Among them, the moisture content (mass percentage) was about 65%, an optimum pH value was 5.5-6.5, and the optimum pH value was 5-6 during the fruiting period.

[0050] Hyphae growth period management: a temperature of a culture room was kept at 14-16? C., a humidity was kept at 60%-70%, and a concentration of CO.sub.2 was controlled below 3,000 ppm. Under these conditions, the bottle could be filled after 22-23 days of cultivation, and hyphae stimulation could be performed after keeping for 1 day. The first 5 days after inoculation were a hyphae germination stage, and the temperature of the culture room was controlled at 18-20? C. After 5 days of hyphae growth, the temperature was automatically adjusted to 14-16? C. by a refrigeration system. A ventilation system was used to keep good ventilation conditions in the culture room, and the ventilation was controlled by CO.sub.2 concentration, so that the CO.sub.2 concentration in the culture room was controlled at about 3,000 ppm, and the ventilation air flow reached every corner of the room to make the hyphae grow uniformly, which was convenient for later management.

[0051] Fruiting management: the hyphae stimulation could be performed after the hyphae were cultured until the bottle was full and being placed for 1-2 days. It was appropriate to remove an aging fungal coat on a surface of the bottle and smooth the surface. The depth was generally at the beginning of a bottle shoulder. After hyphae stimulation, the bottle was to be replenished with water in time by a water replenishing machine. The temperature range of fruiting was adjusted to 5? C.-16? C. and controlled by stages to stimulate the hyphae to recover and form young buds, the humidity was controlled between 98%-99%, the CO.sub.2 concentration was controlled below 2,500 ppm, and an illumination system automatically ran for 8 hours every day on the 4.sup.th day. Under such conditions, the buds could appear after about 3 days. When the buds grew out of a bottle mouth by 2-3 cm, a paper rolling process was carried out. On the eighth day or so, when the buds grew, the temperature was gradually reduced from 15? C. to 6? C. and the humidity was 90%-95%. The 12.sup.th-16.sup.th day was an inhibition period with the temperature being controlled at 3-5? C. and the humidity of 90%, and the illumination system was used for 8 hours every day. After the buds were inhibited, the buds would grow more orderly. When the buds grew 2-3 cm out of the bottle mouth, rolled papers were put on immediately to increase the CO.sub.2 concentration in a small range, thus achieving the effect of promoting the stipe and inhibiting the cap. Then the buds in the bottle were immediately transferred to a fertility room with the system temperature set at 7-9? C. After about 7 days, the Flammlina filiformis could grow to heights of the rolled papers, about 16 cm, and then could be harvested.

[0052] The strain was productively tested in Lingchuan, Jincheng, Shanxi, and Taigu, Jinzhong sequentially, and the main cultivated strain of the parent yellow Flammlina filiformis San Ming No. 1 was used as a control. The results showed that compared with a parent white Flammlina filiformis strain F0012, the Jin Jin No. 1 had an equivalent average yield, an initial harvest period 1-2 days late, and a golden cap. Different from a yellow Flammlina filiformis variety San Ming No. 1 having a yellow stipe and a base being prone to browning during growth, the Jin Jin No. 1 had a white whole stipe and no browing at the base.

[0053] The present disclosure has been described in detail above. For those skilled in the art, the present disclosure can be implemented in a wide range with equivalent parameters, concentrations and conditions without departing from the spirit and scope of the present disclosure and without unnecessary experiments. Although the present disclosure has given specific examples, it is to be understood that the present disclosure can be further improved. In general, according to the principles of the present disclosure, the present application is intended to include any changes, uses or improvements to the present disclosure, including changes made by conventional techniques known in the art, which depart from the disclosed scope in the present application. According to the scope of the following appended claims, some basic features can be applied.