METHOD OF DETECTING AUTO-ANTIBODIES FROM PATIENTS SUFFERING FROM RHEUMATOID ARTHRITIS, A PEPTIDE AND ASSAY KIT

Abstract

Peptides useful in determining the presence of autoantibodies in patients suffering from rheumatoid arthritis are disclosed.

Claims

1. A method to detect the presence or absence of autoantibodies associated with rheumatoid arthritis (RA), which method comprises contacting a sample suspected of containing said antibodies with at least two peptide units for a time sufficiently long to allow a complex to be formed between the at least one of said peptide units and any said autoantibodies, and detecting the presence or absence of the complex peptide unit wherein at least one peptide unit comprises the motif XG, and at least one peptide unit comprises the motif XnonG, wherein X is a citrulline residue or an analog thereof, G is the amino acid glycine and nonG is an amino acid other than glycine.

2. The method of claim 1, wherein nonG is an amino acid selected from H, I, W, S, R, K, Y, M, F, V, P, Cit or an analog thereof.

3. The method of claim 1, wherein at least one of the peptide units is a cyclic peptide unit.

4. The method of claim 1, wherein the peptide unit with XG motif and the peptide unit with XnonG motif are portions of a single peptide.

5. The method of claim 1, wherein the XnonG peptide unit is not derived from (pro)fillaggrin, fibrin, fibrinogen, vimentin, cytokeratin 1 or cytokeratin 9.

6. The method of claim 1, wherein the XG peptide unit is selected from the group consisting of: TABLE-US-00027 (SEQIDNO:1) 0002-27 HQKRGCitGWSRAA (SEQIDNO:2) 0002-29 HQRRVCitGWSRAA (SEQIDNO:3) 0002-31 HQRRICitGGSRAA (SEQIDNO:4) 0002-32 HQRKWCitGASRAA (SEQIDNO:5) 0002-36 HQFRFCitGCitSRAA (SEQIDNO:6) 0002-37 HQKWRCitGRSCitAA and (SEQIDNO:7) 0002-63 HQFRFCitGWSRAA and the XnonG peptide unit is selected from the group consisting of: TABLE-US-00028 (SEQIDNO:8) 0107-32 KPYTVCitKFMRRP (SEQIDNO:9) 0107-35 ARFQMCitHCitRLIR (SEQIDNO:10) 0107-45 YSFVWCitSHARPR (SEQIDNO:11) 0113-30 ARFQMRHCitRLIR and (SEQIDNO:12) 0218-36 RNLRLCitRERNHA

7. A peptide comprising a sequence with the formula (II):
(A1-A2-A3-A4-A5)-Cit-(A6)-(A7-A8-A9-A10-A11)(II) wherein A1-A2-A3-A4-A5 is an amino acid sequence selected from the group consisting of: TABLE-US-00029 (SEQIDNO:13) RHGRQ (SEQIDNO:14) IRCitYK (SEQIDNO:15) HGRQCit (SEQIDNO:16) GRQCitCit (SEQIDNO:17) FQMCitH (SEQIDNO:18) CitWRGM (SEQIDNO:19) ARFQM (SEQIDNO:20) QCitYKW (SEQIDNO:21) KPYTV (SEQIDNO:22) NRLRL (SEQIDNO:23) RRRCitY (SEQIDNO:24) RFKSN (SEQIDNO:25) RGKSN (SEQIDNO:26) RWVSQ (SEQIDNO:27) MKPRY (SEQIDNO:28) KSFVW (SEQIDNO:29) YSFVW (SEQIDNO:30) FQMRH (SEQIDNO:31) RNMNR and (SEQIDNO:32) RMGRP and sequences homologous thereto; A6 represents an amino acid other than glycine; A7-A8-A9-A10-A11 represents an amino acid sequence selected from the group consisting of: TABLE-US-00030 (SEQIDNO:33) KYIIY (SEQIDNO:34) TNRKF (SEQIDNO:35) KWCitKI (SEQIDNO:36) CitRAVI (SEQIDNO:37) RCitGHS (SEQIDNO:38) CitGRSR (SEQIDNO:39) CitYIIY (SEQIDNO:40) CitRLIR (SEQIDNO:41) IERKR (SEQIDNO:42) FMRKP (SEQIDNO:43) FMRRP (SEQIDNO:44) ERNHA (SEQIDNO:45) AVITA (SEQIDNO:46) TPNRW (SEQIDNO:47) TYNRW (SEQIDNO:48) RTPTR (SEQIDNO:49) RIVVV (SEQIDNO:50) HARPR (SEQIDNO:51) RGMCitR and (SEQIDNO:52) IRFPV and sequences homologous thereto.

8. A peptide comprising a sequence with the formula (III):
(B1-B2-B3-B4-B5-B6)-Cit-(B7)-(B8-B9-B10-B11)(III) wherein B1-B2-B3-B4-B5-B6 is an amino acid sequence selected from the group consisting of: TABLE-US-00031 (SEQIDNO:53) INCitRAS (SEQIDNO:54) ICitKRLY (SEQIDNO:55) KCitCitYNI (SEQIDNO:56) RLYFICit (SEQIDNO:57) IRQGAR (SEQIDNO:58) CitERCitVQ (SEQIDNO:59) CitHQRIT (SEQIDNO:60) RICitRVCit (SEQIDNO:61) GRNQRY (SEQIDNO:62) RCitRQHP (SEQIDNO:63) CitCitRCitVA (SEQIDNO:64) RPKQHV (SEQIDNO:65) RKCitGCitR (SEQIDNO:66) RCitCitRNT (SEQIDNO:67) RCitQCitFT (SEQIDNO:68) QLVYLQ (SEQIDNO:69) QYNRFK (SEQIDNO:70) CitLRHIR (SEQIDNO:71) PRCitCitCitK (SEQIDNO:72) RCitQVRY (SEQIDNO:73) GRCitHAH (SEQIDNO:74) ARHVIR (SEQIDNO:75) RCitGHMF (SEQIDNO:76) GRNIRV (SEQIDNO:77) QIFYLCit (SEQIDNO:78) RQGPIA (SEQIDNO:79) GVYLVR (SEQIDNO:80) NCitCitRRV (SEQIDNO:81) KCitRLCitY (SEQIDNO:82) GRRCitCitL and (SEQIDNO:83 RMPHCitH and sequences homologous thereto; B7 represents an amino acid other than glycine; B8-B9-B10-B11 represents an amino acid sequence selected from the group consisting of: TABLE-US-00032 (SEQIDNO:84) CitHRR (SEQIDNO:85) CitIRR (SEQIDNO:86) FRRN (SEQIDNO:87) AQTT (SEQIDNO:88) GYPK (SEQIDNO:89) RPPQ (SEQIDNO:90) GCitRK (SEQIDNO:91) PIPR (SEQIDNO:92) YTIH (SEQIDNO:93) RIKA (SEQIDNO:94) CitRVR (SEQIDNO:95) TRRP (SEQIDNO:96) TIRP (SEQIDNO:97) IKCitR (SEQIDNO:98) RNVV (SEQIDNO:99) CitRRy (SEQIDNO:100) CitRPR (SEQIDNO:101) TRCitCit (SEQIDNO:102) CitCitGR (SEQIDNO:103) LCitRCit (SEQIDNO:104) RVRCit (SEQIDNO:105) VPRT (SEQIDNO:106) YCitFR (SEQIDNO:107) ARCitCit (SEQIDNO:108) RQCitR (SEQIDNO:109) HIRR (SEQIDNO:110) CitMMR (SEQIDNO:111) CitRICit (SEQIDNO:112) VRKS (SEQIDNO:113) PCitCitR and (SEQIDNO:114) CitRRK and sequences homologous thereto.

9. The peptide of claim 7, wherein A6 is selected from the group consisting of Cit, H, I, K, R, S, W, Y, M, F, V and P.

10. The peptide of claim 7, wherein the peptide is a cyclic peptide comprising a ring of at least 8 amino acids.

11. The peptide of claim 10, wherein the ring comprises at least 11 amino acids.

12. The peptide of claim 7 which comprises the peptide sequence TABLE-US-00033 (SEQIDNO:115) RHGRQCitCitKYIIY (SEQIDNO:116) IRCitYKCitITNRKF (SEQIDNO:117) RHGRQCitCitCitYIIY (SEQIDNO:118) ARFQMCitHCitRLIR (SEQIDNO:119) QCitYKWCitKIERKR (SEQIDNO:120) KPYTVCitKFMRKP (SEQIDNO:121) KPYTVCitKFMRRP (SEQIDNO:122) RNLRLCitRERNHA (SEQIDNO:123) RRRCitYCitRAVITA (SEQIDNO:124) RFKSNCitRIPNRW (SEQIDNO:125) RGKSNCitRTYNRW (SEQIDNO:126) RFKSNCitRTYNRW (SEQIDNO:127) RGKSNCitRIPNRW (SEQIDNO:128) RWVSQCitRRIPIR (SEQIDNO:129) MKPRYCitRRIVVV (SEQIDNO:130) KSFVWCitSHARPR (SEQIDNO:131) YSFVWCitSHARPR (SEQIDNO:132) RNMNRCitWRGMCitR, or (SEQIDNO:133) RMGRPCitWIRFPV or an analog thereof.

13. The peptide of claim 8, which comprises a peptide sequence selected from TABLE-US-00034 (SEQIDNO:134) INCitRASCitKCitHRR (SEQIDNO:135) ICitKRLYCitMCitIRR (SEQIDNO:136) KCitCitYNICitCitFRRN (SEQIDNO:137) RLYFICitCitRAQTT (SEQIDNO:138) IRQGARCitRGYPK (SEQIDNO:139) CitERCitVQCitRRPPQ (SEQIDNO:140) CitHQRITCitVGCitRK (SEQIDNO:141) RICitRVCitCitTPIPR (SEQIDNO:142) GRNQRYCitLYTIH (SEQIDNO:143) RCitRQHPCitHRIKA (SEQIDNO:144) CitCitRCitVACitFCitRVR (SEQIDNO:145) RPKQHVCitHTRRP (SEQIDNO:146) RKCitGCitRCitCitTIRP (SEQIDNO:147) RCitCitRNTCitHIKCitR (SEQIDNO:148) RCitQCitFTCitCitRNVV (SEQIDNO:149) QLVYLQCitCitCitRRY (SEQIDNO:150) QYNRFKCitCitCitRPR (SEQIDNO:151) CitLRHIRCitQTRCitCit (SEQIDNO:152) PRCitCitCitKCitRCitCitGR (SEQIDNO:153) RCitQVRYCitCitLCitRCit (SEQIDNO:154) GRCitHAHCitPRVRCit (SEQIDNO:155) ARHVIRCitCitVPRT (SEQIDNO:156) RCitGHMFCitVYCitFR (SEQIDNO:157) GRNIRVCitCitARCitCit (SEQIDNO:158) QIFYLCitCitHRQCitR (SEQIDNO:159) RQGPIACitLHIRR (SEQIDNO:160) GVYLVRCitLCitMMR (SEQIDNO:161) NCitCitRRVCitMCitRICit (SEQIDNO:162) KCitRLCitYCitPVRKS (SEQIDNO:163) GRRCitCitLCitRPCitCitR or (SEQISNO:l61) RMPHCitHCitSCitRRK or an analog thereof.

14. A peptide comprising a sequence with the formula (IV)
(C1-C2)-(C3-C4-C5)-X-G-C6-(C7-C8-C9-C10)(IV) wherein C1-C2 is HQ, GF, EG or GV; C3-C4-05 represents 3 amino acids of which at least 1 is basic, aromatic or V; C6 is a basic or aromatic amino acid, or is A, G, E, P, V, S or Cit or analog thereof; and C7-C8-C9-C10 is SRAA (SEQ ID NO:208), SCitAA (SEQ ID NO:209), RPLD (SEQ ID NO:210), RVVE (SEQ ID NO:211) or PGLD (SEQ ID NO:212); and analogs of the peptide with the formula (IV).

15. The peptide of claim 14, which is a cyclic peptide comprising a ring of at least 8 amino acids.

16. The peptide of claim 15, wherein the ring comprises at least 11 amino acids.

17. The peptide of claim 14, which is TABLE-US-00035 (SEQIDNO:165) HQRKWCitGASRAA (SEQIDNO:166) HQHWRCitGASRAA (SEQIDNO:167) HQFRFCitGCitSRAA (SEQIDNO:168) HQERRCitGESRAA (SEQIDNO:169) HQKWRCitGFSRAA (SEQIDNO:170) HQRWKCitGGSRAA (SEQIDNO:171) HQRRTCitGGSRAA (SEQIDNO:172) HQRRGCitGGSRAA (SEQIDNO:173) HQCitFRCitGHSRAA (SEQIDNO:174) GFFSACitGHRPLD (SEQIDNO:175) HQERGCitGKSRAA (SEQIDNO:176) HQEKRCitGKSRAA (SEQIDNO:177) HQRWLCitGKSRAA (SEQIDNO:178) HQKRNCitGKSRAA (SEQIDNO:179) EGGGVCitGPRVVE (SEQIDNO:180) HQWRHCitGRSCitAA (SEQIDNO:181) HQKWNCitGRSRAA (SEQIDNO:182) HQKFWCitGRSRAA (SEQIDNO:183) HQKCitKCitGRSRAA (SEQIDNO:184) HQKWRCitGRSCitAA (SEQIDNO:185) HQAWRCitGRSCitAA (SEQIDNO:186) HQNQWCitGRSRAA (SEQIDNO:187) HQNSKCitGRSRAA (SEQIDNO:188) HQKRRCitGRSRAA (SEQIDNO:189) HQKRFCitGRSRAA (SEQIDNO:190) HQKRYCitGRSRAA (SEQIDNO:191) HQKRHCitGRSRAA (SEQIDNO:192) HQERACitGSSRAA (SEQIDNO:193) HQEKMCitGVSRAA (SEQIDNO:194) HQKRGCitGWSRAA (SEQIDNO:195) HQRRVCitGWSRAA (SEQIDNO:196) HQWNRCitGWSRAA (SEQIDNO:197) HQQRMCitGWSRAA (SEQIDNO:198) HQSHRCitGWSRAA (SEQIDNO:199) HQFRFCitGWSRAA (SEQIDNO:200) HQKRRCitGWSRAA or (SEQIDNO:201) GVKGHCitGYPGLD or an analog thereof.

18. A peptide comprising (a) a peptide unit with the formula (II) or (III) or analogs thereof and (b) a peptide with the formula (IV) or an analog thereof wherein each peptide unit may be linear or cyclic.

19. A diagnostic test kit for determining the presence or absence of autoantibodies to rheumatoid arthritis, comprising a peptide according to any of claim 7, 8, 14 or 18 or mixtures thereof, together with at least one further reagent.

20. A method to select a peptide for diagnosis of RA, which comprises screening a peptide library with antibodies obtained from patients with RA and wherein the peptide library is selected from a group consisting of: TABLE-US-00036 Lib(1): (SEQIDNO:202) HQEXXCitXXSRAA Wherein X=any amino acid except cysteine and tryptophan; TABLE-US-00037 Lib(2): (SEQIDNOS:203-204) HQXXXCitGXSR/CitAA Wherein X=any amino acid except cysteine but including Citrulline; TABLE-US-00038 Lib(3): (SEQIDNOS:205-206) HQEXXCitXXSR/CitAA Wherein X=any amino acid except cysteine but including Citrulline; and TABLE-US-00039 Lib(4): (SEQIDNO:207) XXXXXXCitXXXXX Wherein X=any amino acid except cysteine but including Citrulline or equivalents thereof.

21. A peptide obtainable by the method of claim 20.

22. The peptide of claim 8, wherein A6 is selected from the group consisting of Cit, H, I, K, R, S, W, Y, M, F, V and P.

23. The peptide of claim 22, wherein the peptide is a cyclic peptide comprising a ring of at least 8 amino acids.

24. The peptide of claim 23, wherein the ring comprises at least 11 amino acids.

25. The method of claim 1 wherein said peptide comprising the motif XG and the peptide comprising the motif XnonG are separate peptides.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0027] FIGS. 1a and 1b show structures of various citrulline analogues.

MODES OF CARRYING OUT THE INVENTION

[0028] This application is not intended as an educational publication on how to become a person skilled in the art. It is therefore limited to providing sufficient information to enable the ordinary person skilled in the art to understand the invention, and to work it, and to understand the scope of protection.

[0029] Preferably the peptide units are recognized by at least 2 of 100 random sera from recognized RA patients. Obviously it is preferred to use peptide units that are recognized by a considerably higher percentage, preferably at least 30%. The number of peptide units in a method according to the invention is preferably 2 or greater than 2.

[0030] Preferably the invention relates to a method as mentioned above, wherein the peptide unit with the XG motif and peptide unit with the XnonG motif are recognized together by at least 10% of the series of sera from patients. Of course, combinations of peptide units resulting in higher sensitivities are preferred. With some combinations of peptide units as described in this document, diagnostic tests can be carried out with sensitivities of 40, 60, 70, 80 or even 85 and more than 90%.

[0031] It has been shown that the sensitivity of the detection can be further increased if at least one of the peptide units is a cyclic peptide unit.

[0032] The peptide unit with XG motif and the peptide unit with XnonG motif are preferably part of a multipeptide. In the context of the present invention, a multipeptide is a molecule comprised of at least two antigenic peptide units, i.e., combinations of peptide units that may or may not be linked by a covalent bond. Such multipeptides may be comprised of linear, branched, cyclic peptide units or a combination of these. Multipeptides may be comprised both of peptide units having the same amino acid sequence, and of peptide units having different amino acid sequences. A multipeptide according to the invention comprises at least 7, preferably at least 10 amino acids, i.e., the peptide units may overlap. It goes without saying that the XG and XnonG motif can not overlap.

[0033] The invention also relates to a XnonG peptide unit, very useful for the method according to the present invention, comprising a sequence with the formula (II):

(A1-A2-A3-A4-A5)-Cit-(A6)-(A7-A8-A9-A10-A11)(II)

[0034] wherein [0035] A1-A2-A3-A4-A5 is an amino acid sequence selected from

TABLE-US-00001 (SEQIDNO:13) RHGRQ (SEQIDNO:14) IRCitYK (SEQIDNO:15) HGRQCit (SEQIDNO:16) GRQCitCit (SEQIDNO:17) FQMCitH (SEQIDNO:18) CitWRGM (SEQIDNO:19) ARFQM (SEQIDNO:20) QCitYKW (SEQIDNO:21) KPYTV (SEQIDNO:22) RNLRL (SEQIDNO:23) RRRCitY (SEQIDNO:24) RFKSN (SEQIDNO:25) RGKSN (SEQIDNO:26) RWVSQ (SEQIDNO:27) MKPRY (SEQIDNO:28) KSFVW (SEQIDNO:29) YSFVW (SEQIDNO:30) FQMRH (SEQIDNO:31) RNMNR (SEQIDNO:32) RMGRP

[0036] and homologous sequences thereof; [0037] A7 an amino acid other than glycine; [0038] A7-A8-A9-A10-A11 an amino acid sequence selected from

TABLE-US-00002 (SEQIDNO:33) KYIIY (SEQIDNO:34) TNRKF (SEQIDNO:35) KWCitKI (SEQIDNO:36) CitRAVI (SEQIDNO:37) RCitGHS (SEQIDNO:38) CitGRSR (SEQIDNO:39) CitYIIY (SEQIDNO:40) CitRLIR (SEQIDNO:41) IERKR (SEQIDNO:42) FMRKP (SEQIDNO:43) FMRRP (SEQIDNO:44) ERNHA (SEQIDNO:45) AVITA (SEQIDNO:46) TPNRW (SEQIDNO:47) TYNRW (SEQIDNO:48) RTPTR (SEQIDNO:49) RIVVV (SEQIDNO:50) HARPR (SEQIDNO:51) RGMCitR (SEQIDNO:52) IRFPV

[0039] and homologous sequences thereof;

[0040] as well as functional analogues of the peptide with the formula (II).

[0041] The invention also relates to a XnonG peptide unit, very useful for the method according to the present invention, comprising a sequence with the formula (III):

(B1-B2-B3-B4-B5-B6)-Cit-(B7)-(B8-B9-B10-B11)(III)

[0042] wherein [0043] B1-B2-B3-B4-B5-B6 is an amino acid sequence selected from

TABLE-US-00003 (SEQIDNO:53) INCitRAS (SEQIDNO:54) ICitKRLY (SEQIDNO:55) KCitCitYNI (SEQIDNO:56) RLYFICit (SEQIDNO:57) IRQGAR (SEQIDNO:58) CitERCitVQ (SEQIDNO:59) CitHQRIT (SEQIDNO:60) RICitRVCit (SEQIDNO:61) GRNQRY (SEQIDNO:62) RCitRQHP (SEQIDNO:63) CitCitRCitVA (SEQIDNO:64) RPKQHV (SEQIDNO:65) RKCitGCitR (SEQIDNO:66) RCitCitRNT (SEQIDNO:67) RCitQCitFT (SEQIDNO:68) QLVYLQ (SEQIDNO:69) QYNRFK (SEQIDNO:70) CitLRHIR (SEQIDNO:71) PRCitCitCitK (SEQIDNO:72) RCitQVRY (SEQIDNO:73) GRCitHAH (SEQIDNO:74) ARHVIR (SEQIDNO:75) RCitGHMF (SEQIDNO:76) GRNIRV (SEQIDNO:77) QIFYLCit (SEQIDNO:78) RQGPIA (SEQIDNO:79) GVYLVR (SEQIDNO:80) NCitCitRRV (SEQIDNO:81) KCitRLCitY (SEQIDNO:82) GRRCitCitL (SEQIDNO:83) RMPHCitH

[0044] and homologous sequences thereof; [0045] B7 is an amino acid other than glycine; [0046] B8-B9-B10-B11 an amino acid sequence selected from

TABLE-US-00004 (SEQIDNO:84) CitHRR (SEQIDNO:85) CitIRR (SEQIDNO:86) FRRN (SEQIDNO:87) AQTT (SEQIDNO:88) GYPK (SEQIDNO:89) RPPQ (SEQIDNO:90) GCitRK (SEQIDNO:91) PIPR (SEQIDNO:92) YTIH (SEQIDNO:93) RIKA (SEQIDNO:94) CitRVR (SEQIDNO:95) TRRP (SEQIDNO:96) TIRP (SEQIDNO:97) IKCitR (SEQIDNO:98) RNVV (SEQIDNO:99) CitRRY (SEQIDNO:100) CitRPR (SEQIDNO:101) TRCitCit (SEQIDNO:102) CitCitGR (SEQIDNO:103) LCitRCit (SEQIDNO:104) RVRCit (SEQIDNO:105) VPRT (SEQIDNO:106) YCitFR (SEQIDNO:107) ARCitCit (SEQIDNO:108) RQCitR (SEQIDNO:109) HIRR (SEQIDNO:110) CitMMR (SEQIDNO:111) CitRICit (SEQIDNO:112) VRKS (SEQIDNO:113) PCitCitR (SEQIDNO:114) CitRRK

[0047] and homologous sequences thereof;

[0048] as well as functional analogues of the peptide with the formula (III).

[0049] The term homologous sequence, as used in connection with A1-A2-A3-A4-A5, A7-A8-A9-A10-A11, B1-B2-B3-B4-B5-B6 and B8-B9-B10-B11, means that at most two amino acids of each amino acid sequence may be replaced by as many other amino acids (including citrulline and/or an analogue thereof), at most two amino acids (including or an analogue thereof) may be introduced and at most two amino acids may be absent.

[0050] The term analogue as used in connection with the peptide of the formula (II) or (III) means that optionally [0051] one or more amino acids may have the D-configuration, [0052] one or more side chains (other than that of citrulline or an analogue thereof) or terminal ends of the peptide may be acetylated, glycosylated, alkylated, or phosphorylated independently of each other, and [0053] one or more amino acids may be replaced by non-natural amino acids.

[0054] A6 and B7 (independent of each other) are preferably selected from Cit, H, I, K, R, S, W, Y, M, F, V and P.

[0055] Specific preferred peptides to be used in the method according to the invention are characterized in that they comprise at least one peptide sequence selected from

TABLE-US-00005 (SEQIDNO:115) RHGRQCitCitKYIIY (SEQIDNO:116) IRCitYKCitITNRKF (SEQIDNO:117) RHGRQCitCitCitYIIY (SEQIDNO:118) ARFQMCitHCitRLIR (SEQIDNO:119) QCitYKWCitKIERKR (SEQIDNO:120) KPYTVCitKFMRKP (SEQIDNO:121) KPYTVCitKFMRRP (SEQIDNO:122) RNLRLCitRERNHA (SEQIDNO:123) RRRCitYCitRAVITA (SEQIDNO:124) RFKSNCitRTPNRW (SEQIDNO:125) RGKSNCitRTYNRW (SEQIDNO:126) RFKSNCitRTYNRW (SEQIDNO:127) RGKSNCitRTPNRW (SEQIDNO:128) RWVSQCitRRTPTR (SEQIDNO:129) MKPRYCitRRIVVV (SEQIDNO:130) KSFVWCitSHARPR (SEQIDNO:131) YSFVWCitSHARPR (SEQIDNO:132) RNMNRCitWRGMCitR, and (SEQIDNO:133) RMGRPCitWIRFPV aswellas (SEQIDNO:134) INCitRASCitKCitHRR (SEQIDNO:135) ICitKRLYCitMCitIRR (SEQIDNO:136) KCitCitYNICitCitFRRN (SEQIDNO:137) RLYFICitCitRAQTT (SEQIDNO:138) IRQGARCitRGYPK (SEQIDNO:139) CitERCitVQCitRRPPQ (SEQIDNO:140) CitHQRITCitVGCitRK (SEQIDNO:141) RICitRVCitCitTPIPR (SEQIDNO:142) GRNQRYCitLYTIH (SEQIDNO:143) RCitRQHPCitHRIKA (SEQIDNO:144) CitCitRCitVACitFCitRVR (SEQIDNO:145) RPKQHVCitHTRRP (SEQIDNO:146) RKCitGCitRCitCitTIRP (SEQIDNO:147) RCitCitRNTCitHIKCitR (SEQIDNO:148) RCitQCitFTCitCitRNVV (SEQIDNO:149) QLVYLQCitCitCitRRY (SEQIDNO:150) QYNRFKCitCitCitRPR (SEQIDNO:151) CitLRHIRCitQTRCitCit (SEQIDNO:152) PRCitCitCitKCitRCitCitGR (SEQIDNO:153) RCitQVRYCitCitLCitRCit (SEQIDNO:154) GRCitHAHCitPRVRCit (SEQIDNO:155) ARHVIRCitCitVPRT (SEQIDNO:156) RCitGHMFCitVYCitFR (SEQIDNO:157) GRNIRVCitCitARCitCit (SEQIDNO:158) QIFYLCitCitHRQCitR (SEQIDNO:159) RQGPIACitLHIRR (SEQIDNO:160) GVYLVRCitLCitMMR (SEQIDNO:161) NCitCitRRVCitMCitRICit (SEQIDNO:162) KCitRLCitYCitPVRKS (SEQIDNO:163) GRRCitCitLCitRPCitCitR (SEQIDNO:164) RMPHCitHCitSCitRRK

[0056] or an analogue thereof.

[0057] Suitable XG peptides to be used in the method according to the invention preferably comprise the sequence with the formula (IV)

(C1-C2)-(C3-C4-C5)-X-G-C6-(C7-C8-C9-C10)(IV)

[0058] wherein

[0059] C1-C2 is HQ, GF, EG or GV;

[0060] C3-C4-05 represents 3 amino acids of which at least 1, and preferably 2 independently of each other are basic, aromatic or V;

[0061] C6 is equal to a basic or aromatic amino acid, or equal to A, G, E, P, V, S or Cit or analogue thereof; and

[0062] C7-C8-C9-C10 is SRAA (SEQ ID NO:208), SCitAA (SEQ ID NO:209), RPLD (SEQ ID NO:210), RVVE (SEQ ID NO:211) or PGLD (SEQ ID NO:212);

[0063] as well as analogues of the peptide with the formula (IV).

[0064] The term analogue as used in connection with the peptide of the formula (IV) means that optionally

[0065] one or more amino acids may have the D-configuration,

[0066] one or more side chains (other than that of citrulline or an analogue thereof) or terminal ends of the peptide independently of each other may be acetylated, glycosylated, alkylated, or phosphorylated; and

[0067] one or more amino acids may be replaced by non-natural amino acids.

[0068] Specific examples of XG peptides suitable to be used in the method according to the invention comprise a sequence selected from

TABLE-US-00006 (SEQIDNO:165) HQRKWCitGASRAA (SEQIDNO:166) HQHWRCitGASRAA (SEQIDNO:167) HQFRFCitGCitSRAA (SEQIDNO:168) HQERRCitGESRAA (SEQIDNO:169) HQKWRCitGFSRAA (SEQIDNO:170) HQRWKCitGGSRAA (SEQIDNO:171) HQRRTCitGGSRAA (SEQIDNO:172) HQRRGCitGGSRAA (SEQIDNO:173) HQCitFRCitGHSRAA (SEQIDNO:174) GFFSACitGHRPLD (SEQIDNO:175) HQERGCitGKSRAA (SEQIDNO:176) HQEKRCitGKSRAA (SEQIDNO:177) HQRWLCitGKSRAA (SEQIDNO:178) HQKRNCitGKSRAA (SEQIDNO:179) EGGGVCitGPRVVE (SEQIDNO:180) HQWRHCitGRSCitAA (SEQIDNO:181) HQKWNCitGRSRAA (SEQIDNO:182) HQKFWCitGRSRAA (SEQIDNO:183) HQKCitKCitGRSRAA (SEQIDNO:184) HQKWRCitGRSCitAA (SEQIDNO:185) HQAWRCitGRSCitAA (SEQIDNO:186) HQNQWCitGRSRAA (SEQIDNO:187) HQNSKCitGRSRAA (SEQIDNO:188) HQKRRCitGRSRAA (SEQIDNO:189) HQKRFCitGRSRAA (SEQIDNO:190) HQKRYCitGRSRAA (SEQIDNO:191) HQKRHCitGRSRAA (SEQIDNO:192) HQERACitGSSRAA (SEQIDNO:193) HQEKMCitGVSRAA (SEQIDNO:194) HQKRGCitGWSRAA (SEQIDNO:195) HQRRVCitGWSRAA (SEQIDNO:196) HQWNRCitGWSRAA (SEQIDNO:197) HQQRMCitGWSRAA (SEQIDNO:198) HQSHRCitGWSRAA (SEQIDNO:199) HQFRFCitGWSRAA (SEQIDNO:200) HQKRRCitGWSRAA (SEQIDNO:201) GVKGHCitGYPGLD

[0069] or an analogue thereof.

[0070] The peptides according to the invention are preferably cyclic peptides of which the ring comprises at least 10 amino acids, and more preferably at least 11 amino acids. The person skilled in the art is acquainted with various methods for the preparation of cyclic peptides and a further explanation is not required.

[0071] According to a most preferred method of the invention, an XG peptide is used in combination with at least one XnonG peptide, wherein the XG peptide is selected from the group comprised of:

TABLE-US-00007 (SEQIDNO:1) 0002-27 HQKRGCitGWSRAA (SEQIDNO:2) 0002-29 HQRRVCitGWSRAA (SEQIDNO:3) 0002-31 HQRRTCitGGSRAA (SEQIDNO:4) 0002-32 HQRKWCitGASRAA (SEQIDNO:5) 0002-36 HQFRFCitGCitSRAA (SEQIDNO:6) 0002-37 HQKWRCitGRSCitAA (SEQIDNO:7 0002-63 HQFRFCitGWSRAA

[0072] and the XnonG peptide is chosen from the group comprised of:

TABLE-US-00008 (SEQIDNO:8) 0107-32 KPYTVCitKFMRRP (SEQIDNO:9) 0107-35 ARFQMCitHCitRLIR (SEQIDNO:10) 0107-45 YSFVWCitSHARPR (SEQIDNO:11) 0113-30 ARFQMRHCitRLIR (SEQIDNO:12) 0218-36 RNLRLCitRERNHA

[0073] Depending on the desired specificity and sensitivity of the diagnostic test and the respective population of rheumatoid arthritis sera under examination, a preferred combination of XG and XnonG peptide units is selected from the group comprised of:

[0074] 0002-27 and 0107-32; 0002-27 and 0107-35; 0002-27 and 0107-45;

[0075] 0002-27 and 0113-30; 0002-27 and 0218-36; 0002-29 and 0107-32; 0002-29 and 0107-35; 0002-29 and 0107-45; 0002-29 and 0113-30; 0002-29 and 0218-36; 0002-31 and 0107-32; 0002-31 and 0107-35; 0002-31 and 0107-45; 0002-31 and 0113-30; 0002-31 and 0218-36; 0002-32 and 0107-32; 0002-32 and 0107-35; 0002-32 and 0107-45; 0002-32 and 0113-30; 0002-32 and 0218-36; 0002-36 and 0107-32; 0002-36 and 0107-35; 0002-36 and 0107-45; 0002-36 and 0113-30; 0002-36 and 0218-36; 0002-37 and 0107-32; 0002-37 and 0107-35; 0002-37 and 0107-45; 0002-37 and 0113-30; 0002-37 and 0218-36; 0002-63 and 0107-32; 0002-63 and 0107-35; 0002-63 and 0107-45; 0002-63 and 0113-30; 0002-63 and 0218-36.

[0076] The above-mentioned combinations were shown to produce an average again in sensitivity of 6%, bringing the total sensitivity of such a combination test of an XG and a XnonG peptide to 75 to 78%.

[0077] The results described in the examples below further showed that the various peptide units also detected different cohorts of sera. An additional gain in sensitivity was achieved by adding a third peptide unit or even a fourth or more peptide units. Depending on the combination of peptide units selected from the above-mentioned groups, a diagnostic test according to the invention allowed sensitivities of 88-92% to be achieved.

[0078] The invention also relates to a multipeptide, characterized in that it is a linear or branched multipeptide, comprising at least two linear or cyclic peptide sequences selected independently of each other from

[0079] a peptide unit selected from peptide units with the formula (II) and (III) and analogues thereof;

[0080] a peptide unit with the formula (IV) and analogues thereof.

[0081] Such a multipeptide is very suitable for use in the method according to the invention. It makes it possible to carry out the method more simply and more reliably since peptides are used in the same known ratio, and extra operations during the assay or during the preliminary work (e.g., coating a microtitre plate with multipeptide) is avoided.

[0082] The invention further relates to a diagnostic kit for determining the presence of autoantibodies from patients suffering from rheumatoid arthritis, wherein the diagnostic kit comprises a peptide or a multipeptide according to the invention, or a mixture thereof, together with at least one further reagent.

[0083] The invention also relates to a peptide or an antibody of an immunotoxin molecule as described above, or a composition thereof for use as a pharmaceutical composition.

[0084] The present invention further relates to a peptide or an antibody of an immunotoxin molecule as described above or a composition thereof for preparing a pharmaceutical composition or a diagnostic agent for rheumatoid arthritis.

[0085] The present invention also relates to the application of a peptide or a composition thereof as described above for preparing a pharmaceutical composition for the treatment of autoimmune diseases by increasing the size of antigen immune complexes, which improves the clarification of the immune complexes formed.

[0086] The present invention therefore also relates to a method for the treatment of rheumatoid arthritis by introducing into the body of a patient requiring such treatment, at least one peptide according to the invention.

[0087] The invention further relates to a method for the selection of a peptide suitable for the diagnosis of RA, wherein a peptide library is screened with antibodies obtained from patients with RA and wherein the peptide library is selected from a group comprised of:

TABLE-US-00009 Lib(1): (SEQIDNO:202) HQEXXCitXXSRAA

[0088] Wherein X=any amino acid except cysteine and tryptophane

TABLE-US-00010 Lib(2): (SEQIDNOS:203-204) HQXXXCitGXSR/CitAA

[0089] Wherein X=any amino acid except cysteine but including Citrulline

TABLE-US-00011 Lib(3): (SEQIDNOS:205-206) HQEXXCitXXSR/CitAA

[0090] Wherein X=any amino acid except cysteine but including Citrulline

TABLE-US-00012 Lib(4): (SEQIDNO:207) XXXXXXCitXXXXX

[0091] Wherein X=any amino acid except cysteine but including citrulline

[0092] or equivalents thereof.

[0093] Finally, the invention relates to a peptide that can be obtained with the aid of the afore-mentioned method to be used in a diagnostic assay.

[0094] The terms a pharmaceutical composition for the treatment or a drug for the treatment or the use of proteins for the preparation of a drug for the treatment relate to a composition comprising a peptide as described above or an antibody binding specifically to the peptide and a pharmaceutically acceptable carrier or excipient (the two terms are interchangeable) for the treatment of diseases as described above. Suitable carriers or excipients with which the ordinary person skilled in the art is familiar are saline, Ringer's solution, dextrose solution, Hank's solution, oils, ethyl oleate, 5% dextrose in saline, substances improving isotonicity and chemical stability, buffers and preservatives. Other suitable carriers include any carrier that does not itself induce the production of antibodies harmful to the individual receiving the composition, such as proteins, polysaccharides, polylactic acids, polyglycol acids, polymeric amino acids and amino acid polymers. The drug may be administered in any manner known to the ordinary person skilled in the art.

[0095] The peptides according to the invention may be labelled (radioactive, fluorescent or otherwise, as well known in the art) or may be provided with a carrier. In this form also such peptides fall within the scope of the invention. For example, a peptide may be coupled to a carrier protein, such as Keyhole Limpet Haemocyanin or bovine serum albumin. Also, the peptide according to the invention may be non-covalently or covalently coupled to a solid carrier, such as a microsphere (gold, polystyrene, etc.), slides, chips, or a wall of a reactor vessel or of a well of a microtitre plate. The peptide may be labelled with a direct or an indirect label. Examples include biotin, fluorescein and an enzyme, such as horseradish peroxidase. All this is generally known to the ordinary person skilled in the art and requires no further explanation.

EXAMPLES

Example 1: Peptide Synthesis

[0096] Citrullinated peptides were synthesised as described by De Koster, H. S., et al. (J. Immunol. Methods, 187, pp. 177-188, (1995)). Beads were used to which the peptide molecules were attached via an amide bond so that after the removal of protective groups, peptide molecules were still attached to the bead. For the synthesis of peptides an automated multiple peptide synthesiser was used (Abimed AMS422, Abimed, Langenfeld, Germany). The incorporated amino acids and the peptide linker 6-aminohexane acid were protected by a Fmoc-group, and to facilitate coupling, the protected amino acids were activated with PyBOP and N-methylmorpholine. Where necessary, the side chains were protected with groups protecting acid-sensitive side chains. The beads used had a diameter of approximately 100 ?m and comprised approximately 100 pmol peptide each. It is estimated that approximately 0.5% of this amount is bound to the outside and is in principle accessible to antibodies.

[0097] For the synthesis in larger quantities, beads were used that were provided with an acid-sensitive linker. The chemistry of peptide synthesis was largely comparable with the one already described above. The peptides were split off with trifluoroacetic acid and isolated by means of ether precipitation, all in accordance with methods well-known in the art and for which the ordinary person skilled in the art requires no further explanation. It is of course also possible to acquire peptides with a desired sequence commercially. The purity and identity of the individual peptides were checked by means of analytical RP-HPLC and time-of-flight mass spectrometry (MALDI-TOF).

[0098] With the aid of the above method 4 peptide libraries were created, each comprising a large number (8?10.sup.6) citrullinated peptides. The formulas below show the amino acid sequence of the peptides in each library:

TABLE-US-00013 Lib(1): (SEQIDNO:202) HQEXXCitXXSRAA

[0099] Wherein X=any amino acid except cysteine and tryptophane

TABLE-US-00014 Lib(2): (SEQIDNOS:203-204) HQXXXCitGXSR/CitAA

[0100] Wherein X=any amino acid except cysteine but including Citrulline

TABLE-US-00015 Lib(3): (SEQIDNOS:205-206) HQEXXCitXXSR/CitAA

[0101] Wherein X=any amino acid except cysteine but including Citrulline

TABLE-US-00016 Lib(4): (SEQIDNO:207) XXXXXXCitXXXXX

[0102] Wherein X=any amino acid except cysteine but including Citrulline.

[0103] These libraries were screened in accordance with the method described below using sera from patients suffering from rheumatoid arthritis.

Example 2: Reaction of the Bead-Coupled Peptides with Sera from Patients Suffering from Rheumatoid Arthritis (RA)

[0104] With the aid of Protein A-Sepharose, IgG was isolated from serum from patients clinically diagnosed to be suffering from RA. The beads were incubated with a solution comprising i) total serum from the patient, or ii) IgG from a patient conjugated to a reporter enzyme (alkaline phosphatase labelling kit; Roche/Boehringer, Mannheim, Germany). For serum (i) a second incubation was carried out with an alkaline phosphatase conjugated anti-human IgG antibody (Dako D0336; Dako Immunoglobulins, Glostrup, Denmark). After each incubation the beads were thoroughly washed with Tris-HCl buffer pH 8.9 (50 mM Tris pH 8.9, 150 mM NaCl, 0.5% Tween? 20).

[0105] The beads with peptides that had bound the most human IgG (after dying with a substrate of alkaline phosphatase that is converted into an insoluble coloured product, these become the most intensely coloured beads) were selected with the aid of a microscope.

Example 3: ELISA

[0106] The most interesting peptides were synthesised in a slightly larger quantity to form a linear and a cyclic variant, and in most cases in a citrulline and arginine (=control) variant. These peptides were tested for reactivity with a series of sera from RA patients. The peptides were coated to ELISA plates and incubated with sera from RA patients. Each serum was tested in duplicate. Sera from healthy persons were used as negative control.

Example 4: The Family of XG Peptides

[0107] By using sera that gave a positive reaction with cfc1 (see PCT/NL97/00624, which is herewith included by way of reference) peptides with glycine were found on position +1 (i.e., C-terminal) in relation to the citrulline residue. Replacing G on +1 by, for example, A (alanine) reduces the reactivity by 65%. In certain peptides, the E (glutamic acid) on position ?3 is preferably not replaced by A (47% loss of activity). Similarly, the Q (glutamine) on ?4, the H (histidine) on ?5 and the S (serine) on +3 often appeared to be advantageous.

[0108] Thus a number of peptide sequences with the combination -XG- were selected, all of which reacted with RA sera. Specific examples of preferred members of this XG family are

TABLE-US-00017 TABLE1 FamilyofXGpeptides: HQRKWCitGASRAA (0002-32) (SEQIDNO:165) HQHWRCitGASRAA (0002-42) (SEQIDNO:166) HQFRFCitGCitSRAA (0002-36) (SEQIDNO:167) HQERRCitGESRAA (020699-2) (SEQIDNO:168) HQKWRCitGFSRAA (0102-74) (SEQIDNO:169) HQRWKCitGGSRAA (0002-28) (SEQIDNO:170) HQRRTCitGGSRAA (0002-31) (SEQIDNO:171) HQRRGCitGGSRAA (0002-33) (SEQIDNO:172) HQCitFRCitGHSRAA (0002-43) (SEQIDNO:173) GFFSACitGHRPLD (0101-7) (SEQIDNO:174) HQERGCitGKSRAA (020699-1) (SEQIDNO:175) HQEKRCitGKSRAA (020699-4) (SEQIDNO:176) HQRWLCitGKSRAA (0002-30) (SEQIDNO:177) HQKRNCitGKSRAA (0002-38) (SEQIDNO:178) EGGGVCitGPRVVE (0101-5) (SEQIDNO:179) HQWRHCitGRSCitAA (0002-34) (SEQIDNO:180) HQKWNCitGRSRAA (0002-24) (SEQIDNO:181) HQKFWCitGRSRAA (0002-25) (SEQIDNO:182) HQKCitKCitGRSRAA (0002-26) (SEQIDNO:183) HQKWRCitGRSCitAA (0002-37) (SEQIDNO:184) HQAWRCitGRSCitAA (0002-40) (SEQIDNO:185) HQNQWCitGRSRAA (0002-44) (SEQIDNO:186) HQNSKCitGRSRAA (0002-45) (SEQIDNO:187) HQKRRCitGRSRAA (0102-76) (SEQIDNO:188) HQKRFCitGRSRAA (0102-77) (SEQIDNO:189) HQKRYCitGRSRAA (0102-78) (SEQIDNO:190) HQKRHCitGRSRAA (0102-79) (SEQIDNO:191) HQERACitGSSRAA (020699-3) (SEQIDNO:192) HQEKMCitGVSRAA (020699-5) (SEQIDNO:193) HQKRGCitGWSRAA (0002-27) (SEQIDNO:194) HQRRVCitGWSRAA (0002-29) (SEQIDNO:195) HQWNRCitGWSRAA (0002-35) (SEQIDNO:196) HQQRMCitGWSRAA (0002-41) (SEQIDNO:197) HQSHRCitGWSRAA (0002-39) (SEQIDNO:198) HQFRFCitGWSRAA (0002-63) (SEQIDNO:199) HQKRRCitGWSRAA (0102-75) (SEQIDNO:200) GVKGHCitGYPGLD (0101-3) (SEQIDNO:201)

[0109] From the above data a consensus sequence is derived of amino acids that appear to have a preference for a particular position. An XG peptide unit being preferably recognized by RA sera may therefore be represented by:

TABLE-US-00018 ?3 ?2 ?1 0 +1 +2 K R R X G R R W W E K

[0110] All these peptides showed good to excellent results. Especially the peptides 0002-35, 0002-36 and 0002-63 were very satisfactory. In most of the cases the cyclic variant of such a peptide reacted even better (see later). Incidentally, this is not always the case. For example, the linear peptide 0101-7 reacted with 55% of the sera. The cyclic variant of this peptide reacted with only 45% of 186 RA-sera. As expected, the arginine variants of these peptides (wherein the citrulline(s) is/are replaced by arginine(s)) did not react with the RA sera.

Example 5: Cyclic Variants of XG Peptides

[0111] The peptides described below were cyclisized in accordance with the cysteine-bromoacetic acid method. Several cyclic variants were tested, with in particular the cyclic variants of peptides 0002-27, 0002-29, 0002-31, 0002-36, and 0002-63 proving to be of interest.

[0112] Ring size: a number of adequately reacting peptides were used to synthesise additional cyclic peptides that each had a different ring size. Of these the 8, 9, 10, 11 and 12-mers were eventually tested. All these peptides were tested in ELISA with 48 RA sera and 8 normal sera respectively. The 8- and 9-mers reacted with some of the sera (with 22% (titre 0.47) and 28% (titre 0.5), respectively, but the titres (the OD-values found) were much lower than when larger rings were used (10-mer 33% with a titre van 0.60; 11- and 12-mer 50% with an average titre of 0.74.

TABLE-US-00019 TABLE 2 Results from serum-analyses using cyclic peptide 0002-36. N % positive sera RA 318 71 Systemic Lupus Erythematodes 24 0 Prim Sj?gren's syndrome 34 3 SSc Scleroderma 10 0 PM, Polymyositis 11 0 Osteoarthritis 29 0 Crohn's disease 40 0 Colitis ulcerosa 40 0 Infectious diseases (malaria, chlamydia, 250 1 mycoplasma, etc.) Psoriasis 10 0 Vasculitis 30 0 Normal human serum 84 0

[0113] The above table represents ELISAs with sera obtained from patients suffering from RA and from patients suffering from various other autoimmune diseases. This shows the high specificity of RA sera for a peptide such as 0002-36. Practically none of the cases showed sera obtained from other autoimmune diseases and material from normal healthy persons to be positive. The arginine variant of peptide 0002-36 (R on position 0 and +2, i.e., instead of Cit in the formula) was also tested and shown to be negative with all tested RA and normal sera.

TABLE-US-00020 TABLE 3 ELISA with 132 RA sera with peptide 36 compared with peptide cfc1 from [PCT/NL97/00624]: peptide cfc1 cfc1 0002-36 0002-36 (linear) (cyclic) (linear) (cyclic) positive sera 48/132 83/132 80/132 94/132 36% 63% 61% 71%

[0114] By using cyclic cfc1 as well as cyclic 0002-36 an additional 2% sensitivity may be gained (total 73%, see Table 4), which for this type of application is a welcome improvement as long as it is not accompanied by a declining specificity. The latter does not seem to be the case. Thus within the XG group of peptides there is diversity with regard to their ability to react with RA antibodies.

TABLE-US-00021 TABLE 4 ELISA with 186 RA sera and cyclic variants of cf1, 0002-36, and 0002-63 peptide cfc1 0002-63 0002-36 cfc1 + 0002-36 (cyclic) (cyclic) (cyclic) (cyclic) positive sera 51% 71% 71% 73%

[0115] Comparing the linear and the cyclic variants of 0002-36 in two cohorts of RA sera (in total 318 sera), the cyclic variant was shown to react more frequently (and better) than the linear variant. Better, because the OD-values found with the cyclic variant were also much higher than with the linear composition. Whether the cyclic variant was created via SS-bonds between two cysteins, or via a thioether formed, for example, by reacting a cysteine and a bromoacetyl group (formed by reacting a thiol in the side chain of a cysteine with an N-terminal bromoacetyl group, forming a thioether), is immaterial. The N-terminal bromoacetyl group is formed, after the synthesis of the peptide, by allowing the bead-bound peptide (peptidyl-resin) to react with the N-hydroxy succinimide ester of bromoacetic acid) in the same peptide. The ring closure occurs in a phosphate-buffered aqueous acetonitril solution, pH=8.

Example 6: The Family of XnonG Peptides

[0116] Surprisingly, there were also peptides found that were not derived from (pro)filaggrin, fibrin, fibrinogen, vimentin, cytokeratin 1 and cytokeratin 9, and that were recognized by RA sera that did not or only slightly react with peptides of the XG family described above. A characteristic of these peptides is that the amino acid and the C-terminal side of the Cit is basic (R, K, H), aromatic (W, Y, F) aliphatic (M, I, V) or Cit, S or P (see Table 5), but not G. Hence the name XnonG family. Remarkably, XnonG peptides comprise relatively many positively charged amino acids on the ?2, ?1, +1 and +2 positions. Thus the antibodies in these RA sera react with a citrulline in a different peptide context.

[0117] The XnonG peptides mentioned all reacted with one or more XG-negative sera. For example, peptides 0107-32, 0107-35, and 0107-45 react with approximately 15-18% of the XG negative sera. An ELISA test based on one or more of these peptides induces a sensitivity increase of at least 5% (up from 73% to 78%) compared with the combination of cyclic cfc1 and cyclic 0002-36. Very importantly, none of the XnonG peptides mentioned reacted with control sera.

TABLE-US-00022 TABLE5 PeptidesoftheXnonGfamily: RHGRQCitCitKYIIY (0107-33) (SEQIDNO:115) RHGRQCitCitCitYIIY (0107-34) (SEQIDNO:116) IRCitYKCitITNRKF (0107-37) (SEQIDNO:117) ARFQMCitHCitRLIR (0107-35) (SEQIDNO:118) QCitYKWCitKIERKR (0107-43) (SEQIDNO:119) KPYTVCitKFMRKP (0107-31) (SEQIDNO:120) KPYTVCitKFMRRP (0107-32) (SEQIDNO:121) RNLRLCitRERNHA (0107-36) (SEQIDNO:122) RRRCitYCitRAVITA (0107-38) (SEQIDNO:123) RFKSNCitRTPNRW (0107-42) (SEQIDNO:124) RGKSNCitRTYNRW (0107-39) (SEQIDNO:125) RFKSNCitRTYNRW (0107-40) (SEQIDNO:126) RGKSNCitRTPNRW (0107-41) (SEQIDNO:127) RWVSQCitRRTPTR (0102-71) (SEQIDNO:128) MKPRYCitRRIVVV (0102-73) (SEQIDNO:129) KSFVWCitSHARPR (0107-44) (SEQIDNO:130) YSFVWCitSHARPR (0107-45) (SEQIDNO:131) RNMNRCitWRGMCitR (0107-30) (SEQIDNO:132) RMGRPCitWIRFPV (0102-72) (SEQIDNO:133) aswellas INCitRASCitKCitHRR 0215-46 (SEQIDNO:134) ICitKRLYCitMCitIRR 0219-44 (SEQIDNO:135) KCitCitYNICitCitFRRN 0222-56 (SEQIDNO:136) RLYFICitCitRAQTT 0222-57 (SEQIDNO:137) IRQGARCitRGYPK 0223-12 (SEQIDNO:138) CitERCitVQCitRRPPQ 150202RA02 (SEQIDNO:139) CitHQRITCitVGCitRK 150202RA03 (SEQIDNO:140) RICitRVCitCitTPIPR 061102RA01 (SEQIDNO:141) GRNQRYCitLYTIH 061102RA02 (SEQIDNO:142) RCitRQHPCitHRIKA 061102RA03 (SEQIDNO:143) CitCitRCitVACitFCitRVR 061102RA05 (SEQIDNO:144) RPKQHVCitHIRRP 061102RA06 (SEQIDNO:145) RKCitGCitRCitCitTIRP 061102RA07 (SEQIDNO:146) RCitCitRNTCitHIKCitR 061102RA08 (SEQIDNO:147) RCitQCitFTCitCitRNVV 061102RA09 (SEQIDNO:148) QLVYLQCitCitCitRRY 061102RAl1 (SEQIDNO:149) QYNRFKCitCitCitRPR 061102RA12 (SEQIDNO:150) CitLRHIRCitQTRCitCit 061102RA14 (SEQIDNO:151) PRCitCitCitKCitRCitCitGR 061102RA15 (SEQIDNO:152) RCitQVRYCitCitLCitRCit 061102RA16 (SEQIDNO:153) GRCitHAHCitPRVRCit 061102RA17 (SEQIDNO:154) ARHVIRCitCitVPRT 181102RA01 (SEQIDNO:155) RCitGHMFCitVYCitFR 181102RA02 (SEQIDNO:156) GRNIRVCitCitARCitCit 181102RA04 (SEQIDNO:157) QIFYLCitCitHRQCitR 221102RA01 (SEQIDNO:158) RQGPIACitLHIRR 221102RA02 (SEQIDNO:159) GVYLVRCitLCitMMR 0218-36 (SEQIDNO:160) NCitCitRRVCitMCitRICit 271102RA01 (SEQIDNO:161) KCitRLCitYCitPVRKS 271102RA02 (SEQIDNO:162) GRRCitCitLCitRPCitCitR 271102RA03 (SEQIDNO:163) RMPHCitHCitSCitRRK 271102RA04 (SEQIDNO:164)

[0118] From the above data it is possible to derive a consensus sequence of amino acids that appear to have a preference for certain positions. Therefore, a XnonG peptide unit preferably recognized by RA sera may be represented by:

TABLE-US-00023 ?5 ?4 3 ?2 ?1 0 +1 +2 +3 +4 +5 R R R R R X R R R R R N Y V Q H T I I K F I Y Y P P H S V N G

[0119] Experiments have shown that, for example, peptide 0107-35 in the cyclic form reacts with 18% of the sera that are not reactive with cyclic 0002-36. This sensitivity may be further increased by other peptides. For example, a further 8% may be added by cyclic peptide 0107-32. This allows the sensitivity to be increased to 80%. A similar value was found for the peptide 0107-45. In total 17 of the 52 (32%) XG-negative sera were shown to react with one or more XnonG peptides. This means that with combinations of more than 2 XG and XnonG peptides, a preferred embodiment of the invention, a sensitivity of above 80% can be achieved. This is therefore better than what can be achieved by a combination of the peptides with the formula IV, and those known from PCT/NL97/00624.

[0120] The sensitivity can be increased even further by using more peptides still. When testing the RA sera with a cyclic XG peptide (for example 0002-63 or 0002-36) together with a linear or cyclic XnonG peptide (for example 0107-35), for 318 RA sera a sensitivity of 78% was obtained. The addition of a 3rd, 4th and possibly 5th peptide increased the sensitivity to 85% (peptides 0107-32, 0107-42 respectively 0107-34). A mere 48 of the 318 RA sera did not react with one of the peptides mentioned.

Example 7: Sensitivity and Specificity of Diagnostic Tests Comprising XG and XnonG Peptide Units

[0121] Using the above-described methods, seven XG peptide units were tested for reactivity with the 318 sera mentioned in Table 2 from patients suffering from rheumatoid arthritis. The specificity was determined with the aid of sera mentioned in Table 2 from control patients and normal donors. Table 6 shows the specificity and sensitivity of the individual peptide units.

TABLE-US-00024 TABLE 6 XG Peptide Sensitivity [%] Specificity [%] units Linear cyclic linear cyclic 0002-27 56 71 98 98 0002-29 57 70 98 98 0002-31 51 69 98 98 0002-32 61 71 98 98 0002-36 61 71 99 99 0002-37 60 70 98 98 0002-63 60 71 98 98 cfc1 36 56

[0122] In accordance with the methods described in this document, five XnonG peptide units were tested for reactivity with the same 318 sera mentioned in Table 2 from patients suffering from rheumatoid arthritis. Specificity was again determined with the aid of the sera mentioned in Table 2 from control patients and normal donors. Table 7 shows the specificity and sensitivity of the individual peptide units.

TABLE-US-00025 TABLE 7 Sensitivity [%] Specificity [%] Peptide unit Linear cyclic linear cyclic 0107-32 48 63 98 98 0107-35 52 61 98 99 0107-45 51 69 98 99 0113-30 49 71 99 100 0218-36 50 70 98 98

[0123] The reactivity of the XnonG peptide units mentioned in Table 7 was tested with 80 sera from the panel of 318 sera described above that did not react with any of the XG peptide from Table 6. The percentages mentioned in table 8 therefore show the percentage of the sera that did comprise antibodies to XnonG peptide units but comprised no XG reactive antibodies.

TABLE-US-00026 TABLE 8 Sensitivity [%] Peptide unit Linear cyclic 0107-32 15 18 0107-35 17 18 0107-45 16 18 0113-30 18 19 0218-36 17 17

[0124] The above results show that an increased sensitivity may be expected if each of the XG peptide units from Table 6 is combined with each of the peptide units from Table 7. All the combinations of peptide units given below were tested with a representative portion of the above-mentioned panel of 318 sera from patients suffering from rheumatoid arthritis. This experiment does in fact show that an average gain in sensitivity of 6% was obtained, bringing the total sensitivity of such a combination test to 75 to 78%.

[0125] The tested combinations of peptide units related to: peptide unit 0002-27 with 0107-32; 0002-27 with 0107-35; 0002-27 with 0107-45; 0002-27 with 0113-30; 0002-27 with 0218-36; 0002-29 with 0107-32; 0002-29 with 0107-35; 0002-29 with 0107-45; 0002-29 with 0113-30; 0002-29 with 0218-36; 0002-31 with 0107-32; 0002-31 with 0107-35; 0002-31 with 0107-45; 0002-31 with 0113-30; 0002-31 with 0218-36; 0002-32 with 0107-32; 0002-32 with 0107-35; 0002-32 with 0107-45; 0002-32 with 0113-30; 0002-32 with 0218-36; 0002-36 with 0107-32; 0002-36 with 0107-35; 0002-36 with 0107-45; 0002-36 with 0113-30; 0002-36 with 0218-36; 0002-37 with 0107-32; 0002-37 with 0107-35; 0002-37 with 0107-45; 0002-37 with 0113-30; 0002-37 with 0218-36; 0002-63 with 0107-32; 0002-63 with 0107-35; 0002-63 with 0107-45; 0002-63 with 0113-30 and finally 0002-63 with 0218-36.

[0126] With respect to the results of Table 6 and Table 8 it should also be noted that the various peptide units also detected different cohorts of sera. From this it may be deduced that the above-mentioned combinations of XG and XnonG peptide units also are capable of producing a further gain in sensitivity if a third peptide unit or even a fourth or further peptide units are added. Depending on the selected combination of peptide units, the diagnostic test according to the invention did indeed make it possible to achieve a sensitivity of 88-92%.