Method of treating polyester textile

Abstract

Provided is an enzymatic treatment on polyester/cellulose blend textile by contacting the textile with a cutinase and preferable with cellulose as well.

Claims

1. A method for manufacturing polyester/cellulose blend textile, comprising the following steps: (a) textile pretreatment, (b) polyester dyeing, (c) cellulosic fiber dyeing, and (d) soaping; wherein a cellulase is added before, during or after step (b), step (c) and/or step (d); and a cutinase is added before, during or after step (b), step (c) and/or step (d).

2. The method of claim 1, wherein the textile is polyester/cotton blend, or polyester/viscose blend.

3. The method of claim 1, wherein the polyester is polyethylene terephthalate (PET).

4. The method of claim 1, wherein step (a) is conducted at temperature 100-120 C.

5. The method of claim 1, wherein the disperse dyestuff is applied in step (b).

6. The method of claim 1, wherein the reactive dyestuff is applied in step (c).

7. The method of claim 1, wherein a surfactant is applied in step (d) for soaping.

8. The method of claim 1, wherein step (d) is conducted at a temperature of 70-80 C.

9. The method of claim 1, wherein step (d) is followed by a step (e) of treating textile with a softening agent.

10. The method of claim 9, wherein the softening agent is selected from the group consisting of soap, vegetable oil, quaternary ammonium salts with alkyl chains, salts of monoesters and diesters of phosphoric acid and the fatty alcohols.

11. The method of claim 1, wherein the cellulase and the cutinase are added during step (d).

12. The method of claim 1, wherein the cellulase and the cutinase are added after step (d).

13. The method of claim 1, wherein the cellulase and the cutinase are added during step (c).

14. The method of claim 1, wherein the cellulase and the cutinase are added before step (b) and after step (a).

15. The method of claim 1, wherein the cellulase and the cutinase are added before step (c) and after step (b).

16. The method of claim 1, wherein the cellulase is added during step (c) and the cutinase is added during step (d).

17. The method of claim 1, wherein the cellulase is added during step (c) and the cutinase is added right after step (d).

18. The method of claim 1, wherein the blend textile comprises at least 5% (w/w) of polyester.

19. The method of claim 1, wherein the cellulase is an endoglucanase.

Description

EXAMPLES

(1) Enzyme

(2) Cellusoft CR) (a Humicola insolens mono-component endoglucanase product commercially available from Nozozymes A/S); Cutinase-1: variant of cutinase from Humicola. Insolens, with substitutions E6Q+A14P+E47K+R51P+E179Q+G8D+N15D+S48E+A88H+N91H+A130V+R189V on the parent H. insolens cutinase of SEQ ID NO:1 (Cutinase-1 described in WO 2001/092502).
Material Disperse dyestuff: Artelon Scallet SW-XG (commercially available from Argus Shanghai Textile Auxiliary Co., LTD); Disperse dyestuff: Red S-2GFL (commercially available from Zhejiang Longsheng Chemical Co., LTD); Reactive dyestuff: Red BF-3B (commercially available from Zhejiang Longsheng Chemical Co., LTD); Phosphate buffer (PBS buffer): Na.sub.2HPO.sub.4, NaH.sub.2PO.sub.4 solutions mixed at specific volume to achieve the target pH; De-oil agent RO-G (commercially available from Argus shanghai Textile Auxiliary CO., LTD); Leveling agent for disperse dyeing Peregal O25 (commercially available from Hebei Xingtai Kewang Auxiliary agent CO., LTD.); Soaping agent Dekol SNS (commercially available from BASF); IPE1310 (surfactant from zhejiang Haian petrochemical plant); INVADINE CWA (surfactant from Huntsman); 32 s TC 65/35 knit: knitted fabric with 65% PET and 35% cotton (s represents the count of yarn weaving knitted fabric) (commercially available from Shanghai Tiqiao textile and yarn dyeing Co., LTD).
Method
Pilling Note Test

(3) Swatches including treated and untreated which had been pre-conditioned in norm climate (65% humidity, 20 C.) for at least 24 hours were tested for the pilling notes with Nu-Martindale Tester (James H. Heal Co. Ltd, England), with untreated fabrics of the same type as the abraded fabrics. A standard pilling test (Swiss Norm (SN) 198525) was carried out after 2000 Revolutions by marking from 1-5, with the meaning defined as below, where 1 shows poor anti-pilling and 5 shows excellent anti-pilling property. Thus the higher the Martindale pilling notes score the more effective the biopolishing treatment. Note 5: No pilling Note 4: Slight Pilling Note 3: Moderate Pilling Note 2: Distinct Pilling Note 1: Heavy Pilling , notes are allowed

(4) Three separate readings were carried out by different persons for each sample, and the average of the three readings was adopted as the final result of pilling notes.

(5) BETEB-Agar Plate for Evaluation of the Cutinase Activity

(6) BETEB was hydrolyzed by cutinase into more soluble agents. Thus, after hydrolysis by enzyme, there were transparent zones on the plates poured with the mixture of Agar and BETEB.

(7) ##STR00001##

(8) Hydrolysis of BETEB will produce

(9) ##STR00002##

(10) Cutinase activity was measured by the below process:

(11) a) BETEB solution preparation: 5 ml 100% ethanol was added into a glass bottle with a plug, 20 mg BETEB was added into the ethanol and then the bottle was placed in a 60 C. water bath to dissolve the BETEB.

(12) b) 1.5% agar solution was prepared by adding 0.75 g agar into 45 ml Tris-HCl buffer (25 mM, pH 7.0), and then placing the baker in a Microwave oven heating twice for 30 seconds to dissolve the Agar.

(13) c) The agar solution was cooled down to 60 C. and mixed with the BETEB solution prepared in step a). The mixture was poured into a petri dish.

(14) d) Small holes were dug in the petri dish with a tip of 6 mm diameter or puncher.

(15) e) Enzyme sample of 30 microgram/ml was added into the petri dish by a tip with 75 microliter (ul) enzyme sample for each hole. The petri dish was placed at 37 C. overnight.

(16) Cutinase-1 showed transparent zones in the area around the holes, as BETEB was hydrolyzed by the cutinase.

Example 1

Cellulase and Cutinase in One Bath and Combined in Soaping in Launder-O-Meter

(17) Small scaled (14 cm*14 cm) fabric of 32 s TC 65/35 knit was treated in Launder-O-Meter (LOM) for biopolishing. Fabric pretreatment was conducted in JFO (Werner Mathis Model JFO Laboratory Jet Dyer) and then fabric was cut into 14 cm*14 cm for polyester disperse dyeing which was carried out in Lab-O-mat (Type BFA Beaker Dyer); followed by reduction clearing and rinse; reactive dyeing of cotton biopolishing was carried out in a SDL-Atlas LP2 Launder-O-Meter (LOM) and followed by soaping. The detailed steps were explained as below:

(18) Step 1. Fabric Pretreatment

(19) First fabric was pretreated in JFO (Mathis Model JFO Laboratory Jet Dyer) to remove the spinning oil or scour and bleach of TC blended fabrics. 1 kg fabric was prepared to a barrel, and then loaded on the device followed by sewing to form a circle. Fabric was arranged in the cavity to make it whirling smoothly.

(20) JFO equipment settings: winch speed 12 m/min, liquor pump 70% of full capacity, turn over 28 seconds. Then start the equipment with 0.4 g/L de-oil agent RO-G, 1 g/L NaOH at 90 C. for 60 min. And then wash at 40 C. for 10 min and then drain out to remove other impurities. Further the fabric was centrifuged and then dried in dryer.

(21) Step 2. Disperse Dyeing, Reduction Clearing and Rinse

(22) Then the fabric was cut into rectangular pieces/swatches of 14 cm14 cm about 4-5 g for disperse dyeing in Lab-o-mat. The temperature increased from 20 C. to 70 C. at 5 C./min and then increased to 135 C. at 1 C./min; the liquor ratio was 10:1; pH was adjusted with acetic acid to 4.5; 1% owf (on weight of fabric) disperse dyestuff Artelon Scallet SW-XG and 1 g/L leveling agent peregal O25 was added when temperature increased to 40 C.

(23) After disperse dyeing, drain the water and reduction clearing was conducted in the conditions of Na.sub.2S.sub.2O.sub.4 (Hydrosulfite) 2 g/L, Na.sub.2CO.sub.3 3 g/L at 70-80 C., 30 min and then followed by rinse at 80 C. for 10 min.

(24) Step 3. Reactive Dyeing

(25) After disperse dyeing, the fabric was colored with dyestuff Red BF-3B at 2% owf at 55 C., pH 7 for 60 min with 80 g/L NaCl. The process was conducted as following: dyestuff and NaCl was first dissolved in the phosphate buffer (Na.sub.2HPO.sub.4, NaH.sub.2PO.sub.4, pH7) and then one piece was placed in each beaker. Buffer (dyestuff and salt has been dissolved in) were added based on the calculation of actual fabric weights, with a liquid to fabric v/w (ml/mg LR ratio) of 10:1.

(26) Each beaker was fitted with a lid lined with 2 neoprin gaskets and close tightly with the metal clamping device. The beakers were loaded into the LOM preheated to 55 C. If cellulase biopolishing is combined in reactive dyeing then small acid proof steel balls were added for mechanical action. Metal racks were used to accommodate and secure 5 beakers, in the vertical position, in each of the 4 drum positions. The LOM lid was closed and dyeing was conducted. 60 min later the fabric was drained out and moved to the soaping step.

(27) Step 4. Soaping

(28) Soaping was conducted in 90 C. for 10 min with 1 g/L SNS. Usually two process of soaping were conducted for middle and dark shade. In example 1, two baths of soaping were conducted.

(29) Enzyme biopolishing was conducted in LOM with PBS Buffer at LR=10. 0.6% owf Cellusoft CR, cutinase-1 at 5.6 mg enzyme protein/gram of fabric was added in one bath for 2 hours at 70 C. and pH 7, 0.1 g/L IPE1310 was added. One piece of fabric was placed in each beaker together with 10 small steel balls (diameter 1.5 cm) providing mechanical aid. Enzyme biopolishing was conducted after step 1, after step 2, after step 3 and after step 4 respectively. After two hours enzymatic treatment, the fabric was rinsed, centrifuged, dried and conditioned for anti-pilling evaluation.

(30) TABLE-US-00001 TABLE 1 Anti-pilling performance of cutinase and cellulase in one bath (pilling was evaluated after soaping) Cellulase and Cellulase and cutinase in one cutinase in one Cellulase and Cellulase and TC 65/35 bath after the bath after cutinase in the cutinase in one knit Untreated pretreatment reduction clearing reactive dyeing bath after soaping Pilling note 1.0 0.2 1.8 0.2 2.2 0.2 2.1 0.1 2.8 0.2 (2000R, Martindale)
The untreated group means the fabrics were only dyed without enzyme treatment. In step 3 there was no cellulase added in while in step 4 there were only two ordinary steps of soaping.
When cellulase Cellusoft CR) and Cutinase-1 were used together in one bath, it was found that the best biopolishing performance was obtained when the biopolishing was conducted at the bath after soaping.

Example 2

Cellulase was Combined in the Reactive Dyeing and Cutinase in the Bath after Soaping (in LOM)

(31) First the fabric pretreatment was conducted in JFO and then fabric of 32s TC 65/35 knit was cut into 14 cm*14 cm for polyester disperse dyeing which was carried out in Lab-O-mat, followed by reduction clearing and rinse; reactive dyeing of cotton was carried out in a SDL-Atlas LP2 Launder-O-Meter (LOM) and followed by soaping. And cutinase biopolishing on small scaled fabric was also conducted in LOM. The detailed steps were the same as steps 1-4 of Example 1.

(32) 0.6% owf Cellulase Cellusoft CRC) was combined in reactive dyeing of step 3 and 10 small steel balls were added to each beaker in LOM to provide mechanical action.

(33) Cutinase biopolishing was conducted in the second soaping step as in step 4. It was conducted in LOM with PBS Buffer, LR=10 and Cutinase-1 of 5.6 mg enzyme protein/gram of fabric was added at pH 8 and 70 C., 0.1 g/L IPE1310 was added. One piece of fabric was placed in each beaker together with 10 small steel balls (diameter 1.5 cm) providing mechanical aid. After 2 hours, the fabric was rinsed with water, centrifuged and dried before evaluation.

(34) TABLE-US-00002 TABLE 2 Anti-pilling performance when cellulase was combined in reactive dyeing and cutinase was in the bath of second soaping Cellulase in reactive dyeing and Cutinase in the bath TC 65/35 knit Untreated of second soaping (2 hours) Pilling note 1.0 0.2 3.0 0.2 (2000R, Martindale)
The untreated group means the fabrics were only dyed without enzyme treatment. In step 3 there was no cellulase added in while in step 4 there were only two ordinary steps of soaping.

Example 3

Cellulase and Cutinase in One Bath and Combined in Soaping in JFO

(35) 1 kg fabric of 32s TC 65/35 knit was prepared to a barrel with 1 m in width, and then it was loaded on the device in JFO (Werner Mathis Model JFO Laboratory Jet Dyer) followed by sewing to form a circle. Fabric was arranged in the cavity to make it whirling smoothly.

(36) Equipment setting: to set the winch speed at 12 m/min, liquor pump 70% of full capacity, to make a turn over about 28 seconds. The pretreatment, reactive dyeing of cotton part and biopolishing of 1 kg fabric was carried out in JFO while polyester disperse dyeing was carried out in Jet-dyer (ALLFIT-10). The processes were the same as steps 1-4 of Example 1.

(37) Enzyme biopolishing was conducted in JFO with Buffer (Na.sub.2CO.sub.3-HAC, pH7), LR=10. And 0.6% owf Cellusoft CR, cutinase-1 of 0.4 mg enzyme protein/gram of fabric was added in one bath in the second soaping of step 4. After 1.5 hour reaction, the fabric was rinsed, centrifuged, dried and then conditioned before evaluation.

(38) TABLE-US-00003 TABLE 3 Anti-pilling performance when cellulase and cutinase were in the bath of second soaping Cellulase and Cutinase in the bath of 32s TC 65/35 knit Untreated second soaping Pilling note (2000R, 1.0 0.2 2.2 0.1 Martindale)
The untreated group means the fabrics were only dyed without enzyme treatment. In step 3 there was no cellulase added in while in step 4 there were only two ordinary steps of soaping.

Example 4

Modified Pretreatment before Biopolishing with Cellulase Combined in Reactive Dyeing and Cutinase in the Bath after Soaping (in LOM)

(39) Fabric of 32s TC 65/35 knit was cut into 14 cm*14 cm and the pretreatment was conducted at 100 C. and 110 C. with NaOH to scour the TC blended fabric in Lab-O-mat; 2 g/L caustic soda, 1.2 g/L H.sub.2O.sub.2, 1 g/L CWA (Huntsman) was added to do scouring and bleaching in one step. Then disperse dyeing (step 2 according to Example 1) which was carried out in Lab-O-mat, followed by reduction clearing and rinse; reactive dyeing (step 3 according to Example 1) of cotton was carried out in a SDL-Atlas LP2 Launder-O-Meter (LOM) and followed by soaping (step 4 according to Example 1).

(40) 1.2% owf Cellulase Cellusoft CRC) was combined in reactive dyeing of step 3 and 10 small steel balls were added to each beaker in LOM to provide mechanical action.

(41) Cutinase biopolishing was conducted in the second soaping step as in step 4. It was conducted in LOM with PBS Buffer, LR=10 and Cutinase-1 of 5.6 mg enzyme protein/gram of fabric was added at pH 8 and 80 C. And 0.1 g/L IPE1310 was added. One piece of fabric was placed in each beaker together with 10 small steel balls (diameter 1.5 cm) providing mechanical aid. After 1.5 h the fabric was rinsed with water, centrifuged and dried before evaluation.

(42) TABLE-US-00004 TABLE 4 Anti-pilling performance when cellulase in reactive dyeing and cutinase in the bath of second soaping with different pretreatments Pilling note (standard Pretreatment Martindale, 2000R) Temperature Cellulase + ( C.) Time (min) NaOH (g/L) Cellulase Cutinase 120 15 2 2.7 0.2 3.5 0 110 15 2 2.8 0 3.5 0 110 15 5 2.5 0 3.6 0.1 100 15 2 2.4 0.1 2.6 0.1 100 15 5 2.5 0 2.9 0.1 100 60 5 2.4 0.1 2.9 0.1 100 60 10 2.5 0 2.8 0

(43) It indicated from the table above that the pilling note on 32s TC 65/35 single jersey has been improved from less than 3.0 to 3.5 when the temperature of pretreatment increased from 100 to 110-120 C.

(44) Hence a pretreatment with caustic soda at 110-120 C. will be preferred for biopolishing of polyester/cellulose blended fabrics.