MULTI-FUNCTIONAL FUSION POLYPEPTIDE, PREPARATION METHOD THEREOF, AND APPLICATION OF SAME

Abstract

The invention discloses a multi-functional fusion polypeptide and its preparation method and application thereof, in the field of biopharmaceutics. The fusion polypeptide of the present invention comprises the domain Pro-(D-Pyr)-(D-Cys)-Bip-Arg-Gly-Glu, Ile-Val-Arg-Arg-Ala-Asp-Arg-Ala-Ala-Val-Pro, Arg-Gly-Asp, and Gly-Gly-Gly-Gly, and can treat human pulmonary fibrosis, lung tissue lesions, lung cancer and other tumors. In a cell model for pulmonary fibrosis, the polypeptide of the present invention can significantly lower the hydroxyproline content and suppress the progression of pulmonary fibrosis. MTT assay shows that the polypeptide of the present invention can inhibit the proliferation multiple human tumor cells. The polypeptide of the present invention is prepared by a synthetic method that is uncomplicated method and offers good application prospects.

Claims

1. A multi-functional fusion polypeptide, comprising domains of Pro-(D-Pyr)-(D-Cys)-Bip-Arg-Gly-Glu, Ile-Val-Arg-Arg-Ala-Asp-Arg-Ala-Ala-Val-Pro, Arg-Gly-Asp, and Gly-Gly-Gly-Gly.

2. A multi-functional fusion polypeptide according to claim 1, comprising at least one of the following sequences: TABLE-US-00008 PolypeptideI: Pro-(D-Pyr)-(D-Cys)-Bip-Arg-Gly-Glu-Gly-Gly-Gly- Gly-Ile-Val-Arg-Arg-Ala-Asp-Arg-Ala-Ala-Val-Pro- Gly-Gly-Gly-Gly-Arg-Gly-Asp; PolypeptideII: Ile-Val-Arg-Arg-Ala-Asp-Arg-Ala-Ala-Val-Pro-Gly- Gly-Gly-Gly-Pro-(D-Pyr)-(D-Cys)-Bip-Arg-Gly-Glu- Gly-Gly-Gly-Gly-Arg-Gly-Asp; PolypeptideIII: Arg-Gly-Asp-Gly-Gly-Gly-Gly-Pro-(D-Pyr)-(D-Cys)- Bip-Arg-Gly-Glu-Gly-Gly-Gly-Gly-Ile-Val-Arg-Arg- Ala-Asp-Arg-Ala-Ala-Val-Pro; PolypeptideIV: Arg-Gly-Asp-Gly-Gly-Gly-Gly-Ile-Val-Arg-Arg-Ala- Asp-Arg-Ala-Ala-Val-Pro-Gly-Gly-Gly-Gly-Pro-(D- Pyr)-(D-Cys)-Bip-Arg-Gly-Glu; PolypeptideV: Ile-Val-Arg-Arg-Ala-Asp-Arg-Ala-Ala-Val-Pro-Gly- Gly-Gly-Gly-Arg-Gly-Asp-Gly-Gly-Gly-Gly-Pro-(D- Pyr)-(D-Cys)-Bip-Arg-Gly-Glu; and PolypeptideVI: Pro-(D-Pyr)-(D-Cys)-Bip-Arg-Gly-Glu-Gly-Gly-Gly- Gly-Arg-Gly-Asp-Gly-Gly-Gly-Gly-Ile-Val-Arg-Arg- Ala-Asp-Arg-Ala-Ala-Val-Pro; wherein Pyr is 3-(3-pyridyl)-L-alanine and Bip is L-4, 4-biphenylalanine.

3. A method for using the multi-functional fusion polypeptide of claim 1, comprising, using the multi-functional fusion polypeptide of claim 1 to generate medicines efficacious in treating human pulmonary fibrosis, pulmonary tissue lesions, lung cancer, and tumor.

4. A method according to claim 3, wherein the pulmonary tissue lesions include bacterial pneumonia, viral pneumonia, mycoplasmal pneumonia, chlamydia pneumonia, protozoal pneumonia and fungal pneumonia.

5. A method according to claim 3, wherein the lung cancer comprises squamous cell carcinoma, adenocarcinoma, glandular scale cancer, small cell lung cancer, non-small cell lung cancer, and large cell carcinoma.

6. A method according to claim 3, wherein the tumor comprises primary or secondary tumor, melanoma, hemangioma, and sarcoma originated from head, neck, brain, thyroid, esophagus, pancreas, liver, stomach, breast, kidney, gallbladder, colon or rectum, ovary, cervix, uterus, prostate, bladder or testis.

7. A method for preparing the multi-functional fusion polypeptide as disclosed in claim 1, wherein the multi-functional fusion polypeptide is synthesized via a solid phase method or a liquid phase method.

8. A method for preparing the multi-functional fusion polypeptide according to claim 7, wherein the solid phase method comprises the steps of: selecting a FMOC-protected, resin-bound amino acid as a starting material; protected amino acids are added one-at-a-time, according to the sequence of the fusion polypeptides, to yield a 29-amino acid polypeptide; the 29-amino acid polypeptide is washed and cleaved from resin to yield a crude fusion polypeptide according to claim 1; and the crude fusion polypeptide is dissolved, purified through a preparative high-performance liquid chromatography, and lyophilized to yield the fusion polypeptide according to claim 1.

9. A method for preparing the multi-functional fusion polypeptide according to claim 7, wherein the liquid phase method comprises the steps of: sequentially connecting amino acids through amide bonds according to the fusion polypeptide sequence, wherein inactive groups of the amino acids are protected by FMOC modification.

10. A method for using the multi-functional fusion polypeptide of claim 2, comprising, using the multi-functional fusion polypeptide of claim 2 to generate medicines efficacious in treating human pulmonary fibrosis, pulmonary tissue, lesions, lung cancer, and tumor.

11. A method according to claim 10, wherein the pulmonary tissue lesions include bacterial pneumonia, viral pneumonia, mycoplasmal pneumonia.

12. A method according to claim 10, wherein the lung cancer comprises squamous cell carcinoma, adenocarcinoma, glandular scale cancer, small cell lung cancer, non-small cell lung cancer, and large cell carcinoma.

13. A method according to claim 10, wherein the tumor comprises primary or secondary tumor, melanoma, hemangioma, and sarcoma originated from head, neck, brain, thyroid, esophagus, pancreas, liver, stomach, breast, kidney, gallbladder, colon or rectum, ovary, cervix, uterus, prostate, bladder or testis.

14. A method for preparing the multi-functional fusion polypeptide as disclosed in claim 2, wherein the multi-functional fusion polypeptide is synthesized via a solid phase method or a liquid phase method.

15. A method for preparing the multi-functional fusion polypeptide according to claim 14, wherein the solid phase method comprises the steps of: selecting a FMOC-protected, resin-bound amino acid as a starting material; protected amino acids are added one-at-a-time, according to the sequence of the fusion polypeptides, to yield a 29-amino acid polypeptide; the 29-amino acid polypeptide is washed and cleaved from resin to yield a crude fusion polypeptide according to claim 2; and the crude fusion polypeptide is dissolved, purified through a preparative high-performance liquid chromatography, and lyophilized to yield the fusion polypeptide according to claim 2.

16. A method for preparing the multi-functional fusion polypeptide according to claim 14, wherein the liquid phase method comprises the steps of: sequentially connecting amino acids through amide bonds according to the fusion polypeptide sequence, wherein inactive groups of the amino acids are protected by FMOC modification.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0026] FIG. 1 is a graph showing the reduction of hydroxyproline content in a pulmonary fibrosis cell model by polypeptides I and II of the present invention;

[0027] FIG. 2 is a graph showing the reduction of hydroxyproline content in a pulmonary fibrosis cell model by polypeptide III and polypeptide IV of the present invention;

[0028] FIG. 3 is a graph showing the reduction of hydroxyproline content in a pulmonary fibrosis cell model by polypeptide V and polypeptide VI of the present invention;

[0029] FIG. 4 is a graph showing the inhibitory effect of the polypeptides I, II, III, IV, V, and VI of the present invention on pulmonary infection;

[0030] FIG. 5 is a graph showing the inhibitory effect of the polypeptides I, II, III, IV, V, VI of the present invention on the growth of lung cancer cells;

[0031] FIG. 6 is a graph showing the inhibitory effects of the polypeptides I, II, III, IV, V, VI of the present invention on different types of tumor growth.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0032] The invention is further described below in conjunction with specific embodiments.

Example 1

Preparation and Test of the Multi-Functional Polypeptide

[0033] In the present embodiment, the polypeptides I-VI are synthesized by a solid phase synthesis method, and are separated and purified by preparative HPLC, and the purity of the polypeptide is determined by an analytical high-performance liquid chromatography.

[0034] The solid phase synthesis method for polypeptides I-VI is based on the solid phase carrier Fmoc-wang-resin (Jill Biochemical Co., Ltd.), wherein protected amino acids are sequentially added to form dipeptide, tripeptide, etc., until the 29-amino acid polypeptide terminal products. After all amino acids are added, the 29-amino acid polypeptide is fully washed, cleaved from the resin, and post-treated to yield a crude product. The crude product is dissolved, purified by preparative high-performance liquid chromatography twice, concentrated and lyophilized to obtain a pure product, and finally purified by a third HPLC purification to obtain a refined polypeptide product. This method not only ensures the efficiency of the synthesis, but also improves the purity of the product.

[0035] The steps for polypeptide synthesis (including elongating peptide from dipeptide to 29- or 17- or 13-amino acid polypeptides) are as follows:

[0036] 1. Weigh 1.5 g of solid phase carrier Fmoc-wang-resin, pour into the glass sand core reaction column, add 5 mL of anhydrous DMF (dimethylformamide) to fully expand the resin for 2 hours, and remove the solvent DMF under reduced pressure.

[0037] a. De-capping: add 15 mL of de-capping solution (containing 20% pyridine, 80% DMF, in volume fraction). After a period of reaction, the de-capping solution is drained, washed once with 15 mL of de-capping solution, and the Fmoc protecting group is removed.

[0038] b. Washing: the de-capping solution was drained, and the resin was washed three times with 15 mL of DMF to thoroughly wash away the by-products.

[0039] c. Condensation: according to the pre-designated amino acid sequence of the polypeptide, the Fmoc-modified monomeric amino acid used for the polypeptide synthesis is dissolved in 5 mL of DMF, 0.5 mL DIEA (N,N-diisopropylethylamine) is added, and the reaction mix is added into a reaction vessel, let react for 2 hours under N.sub.2 blow, remove the reaction solution by filtering, add 5 mL of methanol and perform blocking reaction for 1 hour, wash the resin three times with 15 mL of DCM (dichloromethane).

[0040] d. Washing: drain the reaction solution, wash the resin thoroughly with 15 mL of DMF to wash off by-products.

[0041] e. Cleaving: the dried resin is placed in a round bottom flask, cleavage solution is added to fully cleave the synthesized 29-peptide intermediate, and the resin is separated from the polypeptide by a sand core funnel, wherein the cleavage solution is composed of trifluoroacetic acid:phenol:water:thioanisole:EDT=90:3:3:2:2 by volume.

[0042] 2. The post-treatment steps are as follows: first add anhydrous ether to the cleavage solution to precipitate the polypeptide, then centrifuge, pour off the supernatant, then wash the polypeptide with anhydrous ether, and drain to obtain the crude polypeptide.

[0043] 3. The steps for purification are as follows:

[0044] a. Dissolve: weigh the crude product into a 5-20 g/L solution and filter it with a 0.45 m mixed filter.

[0045] Preparation: perform first purification, second purification and third purification through semi-preparative high-performance liquid chromatography to yield the refined polypeptides. The mobile phase is: phase A acetonitrile, phase B 0.1% TFA aqueous solution.

[0046] 1) First purification: equilibrate the column with 30%-40% acetonitrile in water at a flow rate of 50 mL/min and rinse for 10 min. The crude products after filtering is loaded with an infusion pump at 1 mL/min.

TABLE-US-00001 TABLE I First Purification Elution Gradient Wave Time Flow rate A B length (min) (mL/min) % % nm 0 60 10 90 220 30 60 30 70 220

[0047] Collect solutions with an absorption value greater than 200 my at the wavelength of 220 nm, pool collected solution with a purity greater than 95% as a peak top, which is to be subjected to second purification.

[0048] 2) Secondary purification: equilibrate a column with 30%-40% acetonitrile in water at a flow rate of 50 mL/min and rinse for 10 minutes. The peak top from the first purification was rotary evaporated to remove the organic solvent, and subsequently loaded with an infusion pump at 1 mL/min.

TABLE-US-00002 TABLE 2 Second Purification Elution Gradient Wave Time Flow rate A B length (min) (mL/min) % % nm 0 60 5 95 220 30 60 20 80 220

[0049] Collect solutions with an absorption value greater than 200 my at the wavelength of 220 nm, collect solution with a purity greater than 98% as the qualified solution.

[0050] b. Concentration, filtration, lyophilization: The qualified solution is concentrated under reduced pressure at 37 C. using a rotary evaporator to remove residual solvent and injection water. Finally, filter through a 0.22 m filter, and the filtrate was placed in a lyophilizing tray, and freeze-dried by a lyophilizer to obtain a pure product.

[0051] 3) Third purification: equilibrate a column with 30%-40% acetonitrile in water at a flow rate of 50 mL/min and rinse for 10 min. Purify the samples obtained from the second purification with purity greater than 98% to obtain refined polypeptide.

TABLE-US-00003 TABLE 3 Third Purification Elution Gradient Wave Time Flow rate A B length (min) (mL/min) % % nm 0 60 5 95 220 30 60 15 85 220

[0052] Collect solutions with an absorption value greater than 200 my at the wavelength of 220 nm, pool the collected solution with a purity greater than 99.5% as the refined solution.

[0053] 4. Purity testing. The purified product after lyophilization was collected, and the purity of the polypeptide was examined by analytical RP-HPLC. The analysis conditions were: mobile phase: CAN (+0.1% TFA), H.sub.2O (+0.1% TFA); acetonitrile (CAN) linear gradient: 15%-100%; flow rate: 1.5 mL/min; running time: 30 min; Sample size: 20 L; detection wavelength: 220 nm.

[0054] In this experiment, the solid phase synthesis method was successfully used to synthesize the polypeptide. This method has high reproducibility, high operability, low pollution, and incorporates reversed-phase high-performance liquid chromatography for the purification of the polypeptides. Separation is enhanced using gradient elution compared to isocratic elution. The separation process has a reasonable retention time, high production efficiency, and high purity.

Example 2

[0055] In the present embodiment, the polypeptides I-VI are synthesized by a liquid phase synthesis method, separated and purified by preparative HPLC, and the purity of the polypeptide is determined by an analytical HPLC. The following is the synthetic step of polypeptide I. The synthesis steps of polypeptides II-VI are the same as that of the polypeptide I.

[0056] The polypeptide I synthesis steps are as follows:

[0057] 1. According to the sequence of polypeptide I, 1 mg of the first amino acid Pro and 1 mg of the second amino acid D-Pyr are linked by an amide bond in 10 mL of dichloromethane, and the inactive groups of the amino acid participating in the reaction is modified with Fmoc.

[0058] 2. Add 10 mL of ammonia water to the above reaction system to remove the Fmoc group.

[0059] 3. Repeat the reaction of the first step by adding the third amino acid D-Cys according to the sequence of the polypeptide I and link the third amino acid D-Cys with the dipeptide. The inactive group of the amino acid is modified with Fmoc.

[0060] 4. Repeat steps 2 and 3 until the entire polypeptide sequence Pro-(D-Pyr)-(D-Cys)-Bip-Arg-Gly-Glu-Gly-Gly-Gly-Gly-Ile-Val-Arg-Arg-Ala-Asp-Arg-Ala-Ala-Val-Pro-Gly-Gly-Gly-Gly-Arg-Gly-Asp is synthesized.

[0061] 5. Separate the polypeptide product by preparative HPLC. Mobile phase: linear gradient of acetonitrile: 15%-100%; flow rate: 1.5 mL/min; running time: 60 min; sample loading: 100 mL; detection wavelength: 220 nm.

Example 3

[0062] Establishment of an In Vitro Pulmonary Fibrosis Model

[0063] Human non-small cell lung cancer cells A549 were cultured in DMEM medium containing 10% (volume fraction) fetal bovine serum, cultured in a carbon dioxide incubator with a volume fraction of 5% at 37 C., and changed once every other day, depending on cell density. A549 cells in logarithmic growth phase were digested with 0.25% trypsin to prepare a cell suspension, adjusted to a cell concentration of 110.sup.9 cells/L, and seeded at a density of 410.sup.5 cells/cm2 in a cell culture dish of 90 cm.sup.2. The cells were randomly divided into two groups: (1) control group: cultured in DMEM containing 10% (volume fraction) fetal bovine serum; (2) model group: containing 10% (volume fraction) fetal bovine serum, transforming growth factor (TGF-1, final concentration 5 g/L) was cultured in DMEM. The cells were cultured at 37 C. in a volumetric 5% carbon dioxide incubator, and changed every other day, depending on the cell density. Cell morphology was observed daily using an inverted microscope to determine when the model was established. When A549 cells are changed from the cobblestone shape of the original epithelial cells to the long spindle shape of the interstitial cells, the modeling is successfully established.

Example 4

[0064] Experimental Procedure for Treating Pulmonary Fibrosis with the Fusion Polypeptides

[0065] A549 cells not induced by TGF-1 were used as the control group; the A549 cells induced by TGF-1 and serving as the lung fibrosis model were further divided into multiple groups, one of which does not receive any drug and serves as a model group control, and the rest of the groups were administered with the polypeptides of different doses. After 48 hours of addition of the polypeptides, the cells of the control group, the model group, and the administration group were collected for subsequent measurement of molecular markers related to pulmonary fibrosis.

[0066] Determination of Hydroxyproline Content in Cells

[0067] Human specimen experiments show that a large amount of collagen accumulates in the late stage of pulmonary fibrosis, and the amount of collagen reflects the level of pulmonary fibrosis. Hydroxyproline accounts for 13.4% of collagen, so the content of hydroxyproline can reflect the accumulation of collagen during pulmonary fibrosis, which in turn reflects the degree of pulmonary fibrosis.

[0068] Prepare citric acid buffer: 120 g of sodium citrate, 12 mL of glacial acetic acid, 46 g of citric acid, 34 g of sodium hydroxide, dissolved in distilled water, adjust the pH to 6.0, and then dilute to 1000 mL.

[0069] Prepare 0.05 mol/L chloramine T solution: weigh 7.05 g of chloramine T, dissolve in 100 mL of distilled water, then add 150 mL of ethylene glycol, and add 250 mL of citric acid buffer to mix well.

[0070] Prepare p-dimethylaminobenzaldehyde: Reagent A: Take 20 mL of absolute ethanol, slowly add 2.74 mL of concentrated sulfuric acid; Reagent B: Take 12.0 g of p-dimethylaminobenzaldehyde, slowly add 40 mL of absolute ethanol. Heat in a water bath to completely dissolve and cool to room temperature, then slowly add the A solution to B and mix well.

[0071] Prepare a 3.5 mol/L perchloric acid solution: take 27 mL of perchloric acid and dilute to 100 mL with distilled water.

[0072] The specific steps are as follows: collect the cells in a 1 mL water solution and lyse the cells using a homogenizer; adjust the pH to neutral, dilute to 3 mL, add activated carbon to centrifuge; take 1 mL supernatant, add 1 mL solution 1 (1 mL citrate buffer and 1 mL 0.05 mol/L chloramine T solution), stand for 10 minutes, add 1 mL of perchloric acid, stand for 5 minutes, add 1 mL of p-dimethylaminobenzaldehyde reagent, keep in 60 C. water bath for 15 minutes; centrifuge at 3500 rpm/min for 10 minutes, take the supernatant to measure the absorbance value at a wavelength of 550 nm.

[0073] The test was repeated 3 times independently, and the inhibition rate was calculated as follows: inhibition rate (%)=(1experimental group absorbance/model group absorbance)100%. Table 4 shows the inhibition rate of the hydroxyproline content in the pulmonary fibrosis model of the polypeptides of the present invention. FIG. 1 shows that polypeptides I and II of the present invention can inhibit the content of hydroxyproline in lung fibrosis model cells and inhibit the progression of pulmonary fibrosis; FIG. 2 shows that polypeptide III and polypeptide IV of the present invention can inhibit the content of hydroxyproline in lung fibrosis model cells and inhibit the progression of pulmonary fibrosis; FIG. 3 shows that the polypeptides V and VI of the present invention can inhibit the content of hydroxyproline in lung fibrosis model cells and inhibit the progression of pulmonary fibrosis.

TABLE-US-00004 TABLE 4 Hydroxyproline Content Inhibition Rate in the Pulmonary Fibrosis Model by the Polypeptide of the Present Invention (%) Poly- Poly- Poly- Poly- Poly- Poly- Concen- pep- pep- pep- pep- pep- pep- tration tide I tide II tide III tide IV tide V tide VI 4 uM 36.50 31.28 30.66 33.09 34.47 29.55 8 uM 52.14 57.35 56.21 47.28 49.25 54.17

Example 5

[0074] Inhibition of Various Lung Infections by the Polypeptide of the Present Invention

[0075] The mouse pneumonia model was successfully established by the nose-drop method. To build the mouse pneumonia model, BALB/C mice with a body mass of 16-22 g were selected and anesthetized pneumonia chain was prepared after anesthesia with ether on day 0, day 1, and day 2. Staphylococcal fluid, adenovirus concentrate, Mycoplasma pneumoniae, Chlamydia pneumoniae, protozoa, and pneumonia fungi were slowly dripped into the nasal cavity of the mice, so that they enter the tracheobronchial. During the operation process, care was exercised to prevent bacterial fluid from flowing into the esophagus, which leads to inactivation the bacterial fluid. After successful model establishment, the polypeptides of the present invention were administered, and the results were shown in Table 5. The polypeptide of the present invention displayed a more significant improvement on various lung infections than the penicillin-administered group (FIG. 4). The results were expressed in average valuestandard deviation.

TABLE-US-00005 TABLE 5 Inhibitory Effects of the Polypeptide of the Present Invention on Various Pulmonary Infections (%) Type of Pneumonia Polypeptide I Polypeptide II Polypeptide III Polypeptide IV Polypeptide V Polypeptide VI Penicillin Bacterial 33.15 10.06 43.43 12.38 47.07 13.26 44.75 13.08 38.12 11.17 39.78 9.86 26.52 11.07 Viral 43.78 14.17 57.35 16.34 62.17 17.51 59.10 18.42 50.35 15.73 52.54 13.88 35.02 15.58 Mycoplasma 29.89 12.97 39.16 11.16 42.44 11.96 40.35 16.87 34.37 14.40 35.87 12.71 23.91 14.27 Chlamydia 35.13 13.63 46.02 13.12 49.88 14.05 47.43 17.72 40.40 15.13 42.16 13.36 28.10 14.99 Protozoal 46.25 18.59 60.59 17.27 65.68 18.50 62.44 24.16 53.19 20.63 55.50 18.21 37.00 20.45 Fungal 43.91 15.11 57.52 16.39 62.35 17.56 59.28 19.64 50.50 16.77 52.69 14.80 35.13 16.62 Pneumonia caused by 38.79 16.78 50.81 14.48 55.08 15.52 52.37 21.81 44.61 18.63 46.55 16.44 31.03 18.46 lung infection

Example 6

[0076] MTT Assay for the Inhibition of the Growth of Various Lung Cancer Tumor Cells by the Polypeptide of the Present Invention

[0077] The inhibition of the growth of human lung cancer tumor cells by the polypeptide of the present invention was examined by MTT assay. The lung cancer tumor cells were cultured in a 37 C., 5% (volume fraction) CO.sub.2 incubator to a density of 90% or more, and collected by trypsinization. The cells were resuspended in the culture medium and counted under a microscope to adjust the cell concentration to 2.010.sup.4 cells/mL, the cell suspension was inoculated into a 96-well plate at 100 L per well, and cultured overnight at 37 C. in a 5% (volume fraction) CO.sub.2 incubator. After the cells were completely adhered, the polypeptide of the present invention was added as a drug-administered group, and a culture solution containing no drug was used as a blank control group, and the culture solution was diluted to each predetermined concentration. Each dilution was separately added to a 96-well plate at 100 L per well and incubated for 48 h at 37 C. in a 5% (volume fraction) CO.sub.2 incubator. 20 L of 5 mg/mL MTT was added to each well of a 96-well plate, and incubation was continued for 4 hours. The medium was aspirated, and the cells were dissolved in 100 L of DMSO per well. The absorbance was measured by a microplate reader at a detection wavelength of 570 nm and a reference wavelength of 630 nm, and the growth inhibition rate was calculated as follows: tumor growth inhibition rate (%)=(1administered group absorbance value/unadministered group absorbance value)100%, and the experiment was repeated 3 times independently. The results obtained by the test were expressed as meanstandard deviation. The results in Table 6 indicate that the polypeptides of the present invention have a good inhibitory effect on proliferation of a variety of human lung cancer tumors (FIG. 5).

TABLE-US-00006 TABLE 6 Inhibitory Effects of the Polypeptidesof the Present Inventionon Tumor Growth of Various Lung Cancers Measured by MTT Assay (%) Type of lung cancer Polypeptide I Polypeptide II Polypeptide III Polypeptide IV Polypeptide V Polypeptide VI Docetaxel Squamous cell carcinoma 49.73 9.76 65.14 12.00 70.61 12.86 67.13 12.69 57.18 10.83 59.67 9.56 39.78 10.73 Adenocarcinoma 65.67 13.74 86.03 15.85 93.25 16.99 88.65 17.86 75.52 15.25 78.80 13.47 52.54 15.12 Adenosquamous carcinoma 44.84 12.58 58.73 10.82 63.67 11.60 60.53 16.36 51.56 13.97 53.80 12.33 35.87 13.84 Small Cell Lung Cancer 52.70 13.22 69.03 12.72 74.83 13.63 71.14 17.19 60.60 14.68 63.23 12.96 42.16 14.54 Non-small cell lung cancer 69.38 18.03 90.88 16.75 91.58 17.95 93.66 23.44 79.78 20.01 83.25 17.67 55.50 19.83 Large cell lung cancer 65.87 14.65 86.28 15.90 93.53 17.04 88.92 19.05 75.74 16.27 79.04 14.36 52.69 16.12

Example 7

[0078] MTT Assay for the Inhibition of the Growth of Tumor Cells from a Variety of Different Sources by the Polypeptides of the Invention

[0079] A variety of human tumor cells were cultured in a 37 C., 5% (volume fraction) CO.sub.2 incubator to a density of 90% or more, collected by trypsinization, resuspended in a culture medium and counted under a microscope, and the cells were harvested. The concentration was adjusted to 2.010.sup.4 cells/mL, the cell suspension was inoculated into a 96-well plate at 100 L per well and cultured overnight at 37 C. in a 5% (volume fraction) CO.sub.2 incubator. After the cells were completely adhered, the polypeptides of the present invention were added as drug-administered groups, and a culture solution containing no drug was used as a blank control group, and the culture solution was diluted to each predetermined concentration. Each dilution was separately added to a 96-well plate at 100 L per well and incubated for 48 hours at 37 C. in a 5% (volume fraction) CO.sub.2 incubator. 20 L of 5 mg/mL MTT was added to each well of a 96-well plate and incubation was continued for 4 h. The medium was aspirated and dissolved in 100 L of DMSO per well. The absorbance was measured with a microplate reader at a detection wavelength of 570 nm and a reference wavelength of 630 nm, and the growth inhibition rate was calculated as follows: tumor growth inhibition rate (%)=(1absorbance value of the administered group/unadministered group absorbance value)100%, and the experiment was repeated 3 times independently. The results obtained from the test were expressed as meanstandard deviation, and the tumor growth inhibition rate of the unadministered group was 0. The results in Table 7 indicate that the polypeptides of the present invention have a significant inhibitory effect on the growth of various tumors (FIG. 6).

TABLE-US-00007 TABLE 7 Inhibitory Effects of the Polypeptides of the Present Invention on Various Tumor Growth Measured by MTT Assay (%) Type of cancer Polypeptide I Polypeptide II Polypeptide III Polypeptide IV Polypeptide V Polypeptide VI Docetaxel Head and neck cancer 36.22 10.06 47.44 11.27 55.05 12.07 48.89 13.08 41.65 11.17 43.46 9.86 32.59 9.86 Brain tumor 51.00 14.17 66.81 15.87 77.52 17.00 68.85 18.42 58.65 15.73 61.20 13.88 45.90 13.88 Thyroid cancer 46.70 12.97 61.18 14.53 70.99 15.57 63.05 16.87 53.71 14.40 56.04 12.71 42.03 12.71 Esophageal cancer 49.07 13.63 64.28 15.27 74.58 16.36 66.24 17.72 56.43 15.13 58.88 13.36 44.16 13.36 Pancreatic cancer 54.91 18.59 71.93 20.82 66.99 22.30 74.13 24.16 63.15 20.63 65.89 18.21 49.42 18.21 Liver cancer 54.38 15.11 71.24 16.92 82.66 18.13 73.42 19.64 62.54 16.77 65.26 14.80 48.95 14.80 Gastric cancer 48.41 16.78 63.41 18.79 73.58 20.14 65.35 21.81 55.67 18.63 58.09 16.44 43.57 16.44 Breast cancer 68.27 18.96 89.43 21.24 90.11 22.76 92.16 24.65 78.51 21.05 81.92 18.58 61.44 18.58 Kidney cancer 44.70 15.75 58.56 17.64 67.94 18.90 60.35 20.48 51.41 17.48 53.64 15.44 40.23 15.44 Colorectal cancer 63.55 17.65 83.25 19.77 96.60 21.18 85.80 22.95 73.08 19.60 76.26 17.30 57.20 17.30 Ovarian cancer 67.74 18.82 88.74 21.07 75.87 22.58 91.45 24.46 77.90 20.89 81.29 18.44 60.97 18.44 Cervical cancer 56.16 22.27 62.34 24.94 68.52 26.72 64.58 28.95 64.58 24.72 67.39 21.82 50.54 21.82 Uterine cancer 58.14 19.48 64.54 21.82 70.93 23.38 78.49 25.33 66.86 21.63 69.77 19.09 52.33 19.09 Prostate cancer 60.28 20.08 78.96 22.49 73.54 24.09 81.37 26.10 69.32 22.29 72.33 19.68 54.25 19.68 Bladder Cancer 38.69 10.75 50.68 12.04 58.81 12.90 52.23 13.97 44.49 11.93 46.43 10.53 34.82 10.53 Melanoma 42.41 11.78 55.55 13.19 64.46 14.14 57.25 15.31 48.77 13.08 50.89 11.54 38.17 11.54 Hemangioma 76.08 21.13 84.45 23.67 85.21 25.36 87.49 27.47 87.49 23.46 91.30 20.71 68.47 20.71 sarcoma 49.18 13.66 64.42 15.30 74.75 16.39 66.39 17.76 56.55 15.16 59.01 13.39 44.26 13.39