SAMPLE TRANSFER DEVICE AND METHOD FOR SAMPLE TRANSFER

Abstract

A sample transfer device, an analytical system for analyzing a sample, a method for sample transfer and a method for manufacturing the sample transfer device are disclosed. The sample transfer device includes at least one first block and at least one second block, wherein the first block has at least one first port and at least one second port, wherein the second block has at least one third port and at least one fourth port. The sample transfer device also has at least one slider. The slider is located between the first block and the second block and is configured to slide from a first position to a second position and vice versa. Both in the first position and in the second position a first straight channel is formed between the first port and the third port and a second straight channel is formed between the second port and the fourth port.

Claims

1. A sample transfer device, comprising: a first block comprising a first port and a second port; a second block comprising a third port and a fourth port; a slider located between the first block and the second block, wherein the slider is configured to slide from a first position to a second position and vice versa; wherein both in the first position and in the second position a first straight channel is formed between the first port and the third port and a second straight channel is formed between the second port and the fourth port.

2. The sample transfer device according to claim 1, wherein the slider is a linear slider configured to perform a linear sliding movement from the first position to the second position and vice versa.

3. The sample transfer device according to claim 1, wherein the first block, the second block and the slider each comprise straight channel sections.

4. The sample transfer device according to claim 3, wherein the channel sections all are formed as grooves in surfaces of the first block, the second block and the slider, respectively, wherein the grooves are at least partially covered by at least one cover element.

5. The sample transfer device according to claim 1, wherein the sample transfer device further comprises at least one stopper configured to limit a movement of the slider in linear direction, wherein the position of the at least one stopper defines one or both of the first position or the second position of the slider.

6. The sample transfer device according to claim 1, wherein the sample transfer device further comprises at least one actuator configured to move the slider in a linear direction.

7. The sample transfer device according to claim 1, wherein the slider comprises at least one cavity configured to receive excessive fluid.

8. The sample transfer device according to claim 7, wherein the cavity is located between the first straight channel and the second straight channel while the slider is in the first position.

9. The sample transfer device according to claim 7, wherein the cavity is located on at least one lateral face of the slider perpendicular to a direction of extension of the sample transfer device.

10. An analytical system for analyzing a sample, comprising: the sample transfer device according to claim 1; and at least one analytical device fluidically connected to the sample transfer device.

11. A method for sample transfer using the sample transfer device according to claim 1, the method comprising the following steps: a) feeding a sample into the second straight channel of the sample transfer device; and b) transferring at least one part of the sample to the first straight channel of the sample transfer device by linearly moving the slider from the first position to the second position.

12. The method for sample transfer according to claim 11, wherein step b) is performed when a predetermined analyte of the sample is positioned in a second slider channel section of the second straight channel which is located on the slider.

13. The method for sample transfer according to claim 11, wherein the method for sample transfer further comprises the following step: c) detecting the analyte, wherein the first port and/or the third port are connected to at least one analytical device.

14. A method for manufacturing the sample transfer device according to claim 1, the method comprising the following steps: providing at least one substrate; generating the first straight channel and the least one second straight channel on a surface of the substrate; cutting the substrate in order to form the first block, the slider and the second block, wherein, during the cutting, at least two straight separating lines are generated.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0168] The above-mentioned aspects of exemplary embodiments will become more apparent and will be better understood by reference to the following description of the embodiments taken in conjunction with the accompanying drawings, wherein:

[0169] FIGS. 1A to 1C show an exemplary embodiment of a sample transfer device in different perspective views;

[0170] FIGS. 2A to 2B show an exemplary embodiment of a sample transfer device (FIG. 2A) and a slide view of a slider of the sample transfer device (FIG. 2B);

[0171] FIGS. 3A to 3B show an exemplary embodiment of a sample transfer device (FIG. 3A) and a slide view of a slider of the sample transfer device (FIG. 3B);

[0172] FIGS. 4A to 4E show an exemplary embodiment of a method for sample transfer, wherein an analytical system comprising a sample transfer device is depicted in a top view; and

[0173] FIGS. 5B to 5B show an exemplary embodiment of a method for sample transfer, wherein an analytical system is depicted in a top view.

DESCRIPTION

[0174] The embodiments described below are not intended to be exhaustive or to limit the invention to the precise forms disclosed in the following detailed description. Rather, the embodiments are chosen and described so that others skilled in the art may appreciate and understand the principles and practices of this disclosure.

[0175] FIGS. 1A to 1C show an exemplary embodiment of a sample transfer device 110 in different perspective views. Wherein in FIG. 1A the sample transfer device 110 is shown as a whole, in FIGS. 1B and 1C different enlarged views of the sample transfer device 110 are shown.

[0176] The sample transfer device 110 comprises at least one first block 112, at least one second block 114 and at least one slider 116. The first block 112 comprises at least one first port 118 and at least one second port 120. Further, the second block 114 comprises at least one third port 122 and at least one fourth port 124. The slider 116 is located between the first block 112 and the second block 114. The slider 116 is configured to slide from a first position to a second position and vice versa. Both in the first position and in the second position, a first straight channel 126 is formed between the first port 118 and the third port 122 and a second straight channel 128 is formed between the second port 120 and the fourth port 124. Further, the first block 112 may comprise at least one fifth port 130 and the second block 114 may comprise at least one sixth port 132. In the first position, a further straight channel 134 may be formed between the fifth port 130 and the sixth port 132. Further, the sample transfer device 110 may comprise at least one stopper 136 configured to limit a movement of the slider 116 in linear direction.

[0177] The first block 112 and/or the second block 114 may be made of glass, a ceramic material, a polymer or a semi-conductor material. Still, other materials are feasible. Specifically, the first block 112 and/or the second block 114 may have a cubic shape. The first block 112 and/or the second block 114 may further comprise at least one flat surface 138. Specifically, a top surface 140 of the first block 112 and/or of the second block 114 may be a flat surface 138 which may be configured to provide parts of the first straight channel 126 and the second straight channel 128 as will further be described below. The first block 112 and the second block 114 may have an identical shape. Specifically, the first block 112 and the second block 114 may have an identical height. Further, the first block 112 and the second block 114 may be oriented parallel to each other such that the first port 118 of the first block 112 and the third port 122 of the second block 114 as well as the second port 120 of the first block 112 and the fourth port 124 of the second block 114 may be oriented in one straight line, respectively.

[0178] The slider 116 may be made of a solid material such as glass, a ceramic material, a polymer or a semi-conductor material. Specifically, the slider 114 may be made of the same material as the first block 112 and/or the second block 114. The slider 116 may have a cubic shape. Specifically, the first block 112, the second block 114 and the slider 116 may have an identical high. The slider 116 may comprise at least one flat surface 138. The flat surface 138 may be configured to provide at least parts of the first straight channel 126 and of the second straight channel 128 as will further be described below.

[0179] Specifically, the slider 116 may be a linear slider 142 configured to perform a linear sliding movement from the first position to the second position and vice versa. Therefore, the stopper 136 may be configured to limit a movement of the slider 116 in a linear direction. The stopper 136 may be made of at least one solid material. The stopper 136 may comprise at least one receptacle 144, configured to receive the slider 116 at least partially. A geometry of the receptacle 144 may correspond to the geometry of the slider 116 or at least of parts of the slider 116. The position of the stopper 136 may be adjustable, particularly by micrometer screws and/or clamps (not shown). The position of the stopper 136 may define one or both of the first position or the second position of the slider 116. The sample transfer device 110 may further comprise at least one actuator (not shown) configured to move the slider 116 in a linear direction. Therefore, the stopper 136 may comprise at least one outlet 146 so that the slider 116 may be connectable to the actuator.

[0180] The first block 112, the second block 114 and/or the slider 116 may each comprise straight channel sections 148. The first straight channel 126 may specifically be formed by a first straight channel section 150, located on the first block 112, a second straight channel section 152 located on the second block 114 and a first slider channel section 154 located on the slider 116. The second straight channel 128 may be formed by a third straight channel section 156 located on the first block 112, a fourth straight channel section 158 located on the second block 114 and by a second slider channel section 160 located on the slider 116. The further straight channel 134 may be formed by a fifth straight channel section 162 located on the first block 112, a sixth straight channel section 164 located on the second block 114 and a third slider channel section 166 located on the slider 116. However, the straight channel sections 148 may be exchangeable, such that the first straight channel 126 and/or the second straight channel 128 and/or the further straight channel 134 may be formable with different combinations of channel sections 148. In FIGS. 1A and 1B, the slider 116 may be in the first position. However, as described above, the slider 116 is configured to slide from the first position to the second position and vice versa. In the second position (not shown), the first straight channel 126 may be formed by the first straight channel section 150 located on the first block 112, the straight channel section 152 located on the second block 114 and the second slider channel section 160 located on the slider 116. Further, in the second position, the second straight channel 128 may be formed by the third straight channel section 156 located on the first block 112, the fourth straight channel section 158 located on the second block 114 and the third slider channel section 166 located on the slider 116.

[0181] The straight channel sections 148 may be oriented essentially parallel. Further, the first straight channel and the second straight channel, as well as the second straight channel and the further straight channel, may be separated by a distance of 5 mm to 50 mm. However, even higher distances between the first straight channel 126 and the second straight channel 128, as well as between the second straight channel 128 and the further straight channel 134 may be possible and there may be no limitations.

[0182] The first straight channel 126 and/or the second straight channel 128 and/or the further straight channel 134 may be microfluidic channels 167 with dimensions on a submillimeter scale. As specifically depicted in FIG. 1C, the first straight channel 126 and/or the second straight channel 128 and/or the further straight channel 134 may at least partially have a round cross-section. Specifically, the cross-section may have a diameter of 5 m to 500 m, preferably of 20 m to 100 m. Still, other dimensions are feasible.

[0183] The straight channel sections 148 may be open channels 168. Specifically, the open channels 168 may be formed by a groove 170 into the top surface 140 of the first block 112, the second block 114 and/or the slider 116. As depicted in FIG. 1C, the open channels 168 may be at least partially covered by at least one cover element 172. Cover element 172 may specifically be made of an optically transparent material such as glass. However, other materials are also feasible. The cover element 172 may be a common cover element 174 for the first block 112, the second block 114 and the slider 116.

[0184] FIG. 2A shows an exemplary embodiment of a sample transfer device 110 and FIG. 2B shows a side view of a slider 116 of the sample transfer device 110. The sample transfer device 110 may, at least in large part, correspond to the sample transfer device 110 as illustrated in FIGS. 1A to 1C. Consequently, reference can be made to the description of FIGS. 1A to 1C above.

[0185] Further, the slider 116 may comprise at least one cavity 176, configured to receive excessive fluid. The cavity 176 may be formed by a void volume 178. Specifically, the cavity 176 may be located on at least one lateral face 180 of the slider 116 perpendicular to a direction of extension 182 of the sample transfer device 110. Specifically, the slider may comprise a first slider front face 184 facing the first block 112. The slider 116 may further comprise a second slider front face 186 facing the second block 114. The first slider front face 184 may slide on a first front face 188 of the first block and the second slider front face 186 may slide on a second front face 190 on the second block 114. The cavity 176 may specifically be located on one or both of the first slider front face 184 or the second slider front face 186.

[0186] The cavity 176 may exemplarily have a rectangular cross-section. The cavity 176 may specifically have dimensions in the submillimeter range.

[0187] FIG. 3A shows an exemplary embodiment of a sample transfer device 110 and FIG. 3B shows a side view of slider 116 of the sample transfer device 110. The sample transfer device 110 may, at least in large part, correspond to the sample transfer device 110 as illustrated in FIGS. 2A and 2B. Consequently, reference may be made to the description of FIGS. 2A and 2B above.

[0188] The slider 116 as illustrated in FIGS. 3A and 3B may also comprise the at least one cavity 176 as depicted in FIGS. 2A and 2B. However, the cavity 176 of the slider 116 as illustrated in FIGS. 3A and 3B may fully penetrate the lateral face 180 of the slider 176.

[0189] FIGS. 4A to 4E show an exemplary embodiment of a method for sample transfer. Thereby, an analytical device 192 is illustrated in a top view. The analytical device 192 comprises the sample transfer device 110. The sample transfer device 110 may correspond in large part to the sample transfer device 110 as illustrated in FIGS. 1A to 3B. Thus, reference may be made to the descriptions of the FIGS. 1A to 3B above.

[0190] The analytical system 192 may further comprise at least one analytical device 194 fluidically connected to the sample transfer device 110. Analytical device 194 may be handled independently from the sample transfer device 110. Exemplarily, the analytical system 192 may comprise at least three analytical devices 194. At least one first analytical device 196 may be connected to the first block 112 and the second block 114. Specifically, the first analytical device 196 may be connected to the second port 120 of the first block 112 and to the fourth port 124 of the second block 114. Further, at least one second analytical device 198 may be connected to the first block 112, specifically to the first port 118 of the first block 112. The first analytical device 196 and the second analytical device 198 may be identical types of analytical devices 194. Exemplarily, the first analytical device 196 and the second analytical device 198 may be or may comprise at least one capillary electrophoresis device 200. The analytical system 192 may further comprise a third analytical device 202. The third analytical device may specifically be connected to the second block, specifically to the third port 122 of the first block 112. Exemplarily, the third analytical device 202 may be a mass spectrometer 204. The analytical devices 194, specifically the first analytical device 196, the second analytical device 198 and/or the third analytical device 202 may respectively be connected to the sample transfer device 110 via at least one capillary 206. Moreover, the further straight channel 134, specifically the fifth port 130 and the sixth port 132 may be connected to at least one pump 208 via the capillaries 206.

[0191] In a first step of the method for sample transfer, as depicted in FIG. 4B via first arrows 209, the first straight channel 126 and/or the second straight channel 128 and/or the further straight channel 134 may be rinsed simultaneously with at least one fluid. Thereby, the slider 116 may be in the first position such that the first straight channel 126 may be formed by the first slider channel section 154 and the second straight channel 128 may be formed by the second slider channel section 160.

[0192] In a next step, as depicted in FIG. 4C via a second arrow 211, a sample may be injected into the second straight channel 128, particularly by at least one capillary 206 which is connected to the second port 120 of the sample transfer device 110. Specifically, the sample may comprise at least one analyte 210 as schematically illustrated as a dot in FIG. 4C. An electrical potential may be applied to the second straight channel 128 by the capillary electrophoresis device 200 during feeding the sample into the second straight channel 128 of the sample transfer device.

[0193] In a next step, as depicted in FIG. 4D, at least one part of the sample may be transferred to the first straight channel 126 of the sample transfer device 110 by linear moving of the slider 116 from the first position to the second position as depicted via a third arrow 213. Thereby, the first straight channel 126 may be formed by the second slider channel section 160. Specifically, this step may be performed when the predetermined analyte 210 of the sample is positioned on the second slider channel section 160. The position of the analyte 210 within the second straight channel 128 may be determined by using at least one optical technique (not shown).

[0194] In a next step, as depicted in FIG. 4E, a separation of the analyte 210 may be conducted by applying an electrical potential through the first straight channel 126 by the second analytical device 198, specifically by the capillary electrophoresis device 200. The analyte 210 may be moved into a direction of the mass spectrometer 204. This is illustrated by the fourth arrow 215. The method for sample transfer may further comprise detecting the analyte 210, specifically by the mass spectrometer 204. In a next step (not shown), the slider 116 may be moved back in the first position and the method may be conducted repeatedly.

[0195] FIGS. 5A to 5B show an exemplary embodiment of a method for sample transfer. Thereby, the analytical system 192 is depicted in a top view. The analytical system 192 comprises the sample transfer device 110 and several of the analytical devices 192.

[0196] The sample transfer device 110 corresponds, at least partially, to the sample transfer device 110 as described in FIGS. 1A to 4E. Thus, reference may be made to the descriptions of FIGS. 1A to 4E above. Further, the sample transfer device 110 may comprise three sliders 116. Specifically, the sample transfer device 110 may comprise a first further slider 212 and a second further slider 214. The first further slider 212, the second further slider 214 and the slider 116 may be oriented in a parallel fashion. The first further slider 212, the second further slider 214 and the slider 116 may be located adjacent to each other in between the first block 112 and the second block 114.

[0197] The first block 112 may comprise at least one seventh port 216 and at least one eight port 218. The second block 114 may comprise at least one ninth port 220 and at least one tenth port 222. In the first position, as depicted in FIG. 5A, a third straight channel 224 may be defined between the seventh port 216 and the ninth port 220 and a fourth straight channel 226 may be defined between the eighth port 218 and the tenth port 222. The third straight channel 224 and/or the fourth straight channel 226 may exemplarily be configured to provide at least one fluid for rinsing, digestion, complexation, stacking, dynamic pH junction, transient isotachophoresis or derivatization. Still, other embodiments are feasible. Thus, further pumps 208 may be connected to the seventh port 216 and/or to the eight port 218.

[0198] In the first position, the first straight channel 126 may comprise the first straight channel section 150, the second straight channel section 152, the first slider channel section 154 as well as the first further slider channel section 228 and the second further slider channel section 230. Additionally, the second straight channel 128 may comprise the third straight channel section 156, the fourth straight channel section 158, the second slider channel section 160 as well as the third further slider channel section 232 and the fourth further slider channel section 234. The further straight channel 134 may comprise the fifth straight channel section 162, the sixth straight channel section 164, the third slider channel section 166 as well as the fifth further slider channel section 236 and the second further slider channel section 238.

[0199] The third straight channel 224 may comprise a seventh straight channel section 240 located on the first block 112 and a eight straight channel section 242 located on the second block 114. Further, the third straight channel 224 may comprise a fourth slider channel section 244 located on the slider 116, a seventh further slider channel section 246 located on the first further slider 212 and an eighth further slider channel section 248 located on the second further slider 214. The fourth straight channel 226 may comprise a ninth straight channel section 250 located on the first block 112 and a tenth channel section 252 located on the second block 114. Further, the fourth straight channel 226 may comprise a fifth slider channel section 254 located on the slider 116, a ninth further slider channel section 256 located on the first further slider 212 and a tenth further slider channel section 258 located on the second further slider 214.

[0200] The method for sample transfer may be conducted analogous to the method described within the FIGS. 4A to 4E including feeding a sample into the second straight channel 128 of the sample transfer device 110 until the predetermined analyte 210 of the sample is positioned in the second straight channel slider section 160, as illustrated in FIG. 5A. In a further step, at least one part of the sample, specifically the analyte 210, may be transferred to the first straight channel 126 of the sample transfer device 110 by linearly moving the slider 116, the first further slider 212 and the second further slider 214 such that, in the second position, the first straight channel 126 may be formed by different channel sections of the sliders 116. Thus, the first straight channel 126 may be formed by the first straight channel section 150, the seventh further channel section 246, the second slider channel section 160, the tenth further channel section 258 and the second straight channel section 152. The second straight channel 128 may be formed by the third straight channel section, 156, first straight channel section 228, third slider channel section 166, eight further channel section 248, fourth straight channel section 158.

[0201] While exemplary embodiments have been disclosed hereinabove, the present invention is not limited to the disclosed embodiments. Instead, this application is intended to cover any variations, uses, or adaptations of this disclosure using its general principles. Further, this application is intended to cover such departures from the present disclosure as come within known or customary practice in the art to which this invention pertains and which fall within the limits of the appended claims.

LIST OF REFERENCE NUMBERS

[0202] 110 sample transfer device [0203] 112 first block [0204] 114 second block [0205] 116 slider [0206] 118 first port [0207] 120 second port [0208] 122 third port [0209] 124 fourth port [0210] 126 first straight channel [0211] 128 second straight channel [0212] 130 fifth port [0213] 132 sixth port [0214] 134 further straight channel [0215] 136 stopper [0216] 138 flat surface [0217] 140 top surface [0218] 142 linear slider [0219] 144 receptacle [0220] 146 outlet [0221] 148 straight channel section [0222] 150 first straight channel section [0223] 152 second straight channel section [0224] 154 first slider channel section [0225] 156 third straight channel section [0226] 158 fourth straight channel section [0227] 160 second slider channel section [0228] 162 fifth straight channel section [0229] 164 sixth straight channel section [0230] 166 third slider channel section [0231] 167 microfluidic channel [0232] 168 open channel [0233] 170 groove [0234] 172 cover element [0235] 174 common cover element [0236] 176 cavity [0237] 178 void volume [0238] 180 lateral face [0239] 182 direction of extension [0240] 184 first slider front face [0241] 186 second slider front face [0242] 188 first front face [0243] 190 second front face [0244] 192 analytical system [0245] 194 analytical device [0246] 196 first analytical device [0247] 198 second analytical device [0248] 200 capillary electrophoresis device [0249] 202 third analytical device [0250] 204 mass spectrometer [0251] 206 capillary [0252] 208 pump [0253] 209 first arrow [0254] 210 analyte [0255] 211 second arrow [0256] 212 first further slider [0257] 213 third arrow [0258] 214 second further slider [0259] 215 fourth arrow [0260] 216 seventh port [0261] 218 eighth port [0262] 220 ninth port [0263] 222 tenth port [0264] 224 third straight channel [0265] 226 fourth straight channel [0266] 228 first further slider channel section [0267] 230 second further slider channel section [0268] 232 third further slider channel section [0269] 234 fourth further slider channel section [0270] 236 fifth further slider channel section [0271] 238 sixth further slider channel section [0272] 240 seventh straight channel section [0273] 242 eighth straight channel section [0274] 244 fourth slider channel section [0275] 246 seventh further slider channel section [0276] 248 eighth further slider channel section [0277] 250 ninth straight channel section [0278] 252 tenth straight channel section [0279] 254 fifth slider channel section [0280] 256 ninth further slider channel section [0281] 258 tenth further slider channel section