PASSION FLOWER SEED EXTRACT, AND COSMETIC, PHARMACEUTICAL OR DERMATOLOGICAL COMPOSITIONS CONTAINING SAME

Abstract

The invention relates to a polyphenolic extract of passion flower seeds, in particular Passiflora incarnata or Passiflora edulis seeds, comprising at least 30 percent by weight polyphenols, expressed as gallic acid equivalent, relative to the weight of the dry extract. The invention also relates to a method for preparing an extract of said type, a composition containing same, and the cosmetic, dermatological or therapeutic use thereof.

Claims

1-12. (canceled)

13. A polyphenolic extract of seeds of passion flower, in particular of Passiflora incarnata or Passiflora edulis, comprising at least 30 wt % polyphenols, expressed as gallic acid equivalents, relative to the weight of the dry extract, and at least 10 wt % organic acids, relative to the weight of the dry extract.

14. The extract of claim 13, comprising at least 35 wt % polyphenols, expressed as gallic acid equivalents, relative to the weight of the dry extract.

15. The extract of claim 13, wherein at least 50 wt % of said polyphenols are catechin derivatives, expressed as gallic acid equivalents, relative to the weight of polyphenols in the dry extract.

16. The extract of claim 13, wherein said organic acids are acetic acid, malic acid, citric acid or mixtures thereof.

17. The extract of claim 13, characterized in that it is obtained by solid/liquid extraction of passion flower seeds in a solvent selected from water, glycerols, glycols, and mixtures thereof.

18. The extract of claim 13, characterized in that it is obtained by solid/liquid extraction of passion flower seeds in a solvent selected from the binary mixtures water/glycerol, water/glycol, and mixtures thereof.

19. A method for preparing a polyphenolic extract of passion flower seeds as defined in claim 13, said method comprising at least one step of solid/liquid extraction in a solvent selected from water, glycerols, glycols, and mixtures thereof.

20. The method of claim 19, characterized in that it comprises the following successive steps: a) grinding the seeds; b) optionally defatting the seeds, preferably by pressing, by ethanolic extraction or by supercritical CO.sub.2 extraction; c) solid/liquid extraction of the ground and optionally defatted seeds in a solvent selected from water, glycerols, glycols, and mixtures thereof; d) separating the solid phase and the liquid phase by decantation, and/or centrifugation and/or successive filtrations; and e) optionally drying the extract obtained in step d).

21. The method of claim 19, characterized in that said solid/liquid extraction solvent is selected from the binary mixtures water/glycerol, water/glycol, and mixtures thereof.

22. A composition comprising as active principle a polyphenolic extract of passion flower seeds comprising at least 30 wt % polyphenols, expressed as gallic acid equivalents, relative to the weight of the dry extract, and at least 10 wt % organic acids, relative to the weight of the dry extractor an extract obtainable by the method of claim 19, and a suitable excipient.

23. The composition of claim 22, comprising from 0.001 to 10 wt %, advantageously from 0.01 to 5 wt %, of said polyphenolic extract of passion flower seeds, the weight of the extract being expressed as dry extract, relative to the total weight of the composition.

24. A method for preventing and/or treating: disorders or pathologies of the skin and/or of the mucous membranes and/or of the skin appendages, advantageously inflammatory reactions, oxidation reactions, disorders relating to radical attacks optionally linked to pollution, disorders of the barrier or of homeostasis, of ageing, notably of chronological and/or actinic ageing, of the skin and/or of the mucous membranes and/or of the skin appendages, and/or vascular disorders, and/or damaged adipose tissue, said method comprising the administration of a polyphenolic extract of seeds of passion flower comprising at least 30 wt % polyphenols, expressed as gallic acid equivalents, relative to the weight of the dry extract, and at least 10 wt % organic acids, relative to the weight of the dry extract or an extract obtainable by the method of claim 19 to a subject in need thereof.

25. A method for cosmetic care of the skin and/or of the skin appendages and/or of the mucous membranes, for the purpose of improving the condition and/or the appearance thereof, consisting in administering a polyphenolic extract of seeds of passion flower comprising at least 30 wt % polyphenols, expressed as gallic acid equivalents, relative to the weight of the dry extract, and at least 10 wt % organic acids, relative to the weight of the dry extractor an extract obtainable by the method of claim 19.

26. The extract of claim 13, comprising at least 40 wt %, polyphenols, expressed as gallic acid equivalents, relative to the weight of the dry extract.

27. The extract of claim 13, characterized in that it is obtained by solid/liquid extraction of passion flower seeds in a solvent selected from the binary mixtures water/glycerol, water/glycol, and mixtures thereof, in a proportion of 30% to 90% of glycerol and/or of glycol in water.

28. The method of claim 19, characterized in that said solid/liquid extraction solvent is selected from the binary mixtures water/glycerol, water/glycol, and mixtures thereof in a proportion of 30% to 90% of glycerol and/or of glycol in water.

29. The method of claim 19, characterized in that said solid/liquid extraction solvent is selected from the binary mixtures water/glycerol, water/glycol, and mixtures thereof in a proportion of 50% to 90% of glycerol and/or of glycol in water.

30. The method of claim 19, characterized in that said solid/liquid extraction solvent is selected from the binary mixtures water/glycerol, water/glycol, and mixtures thereof in a proportion of 60% to 80% of glycerol and/or of glycol in water.

31. The method of claim 19, characterized in that said solid/liquid extraction solvent is selected from the binary mixtures water/glycerol, water/glycol, and mixtures thereof in a proportion of 70% of glycerol and/or of glycol in water.

32. The composition of claim 22, comprising from 0.01 to 5 wt %, of said polyphenolic extract of passion flower seeds, the weight of the extract being expressed as dry extract, relative to the total weight of the composition.

Description

DESCRIPTION OF THE FIGURES

[0094] FIG. 1 shows the percentage of Nrf2 activation by an extract of the invention.

[0095] FIG. 2 shows claudin-4 immunostaining in skin explants treated with BaP (Protocol 1)

[0096] FIG. 3 shows filaggrin immunostaining in skin explants treated with BaP+nicotine (Protocol 2)

[0097] FIG. 4 shows loricrin immunostaining in skin explants treated with BaP+nicotine (Protocol 2)

[0098] FIG. 5 shows collagen-I immunostaining in skin explants treated with BaP+nicotine (Protocol 2)

[0099] FIG. 6 shows elastin immunostaining in skin explants treated with BaP (Protocol 1)

[0100] FIG. 7 shows fibronectin immunostaining in skin explants treated with BaP (Protocol 1)

[0101] FIG. 8 shows the visual appearance of the comet assay after electrophoresis of treated normal human keratinocytes. FIG. 8A corresponds to a negative control sample, FIG. 8B to a UV control sample and FIG. 8C to a 0.001% extract of the invention.

[0102] FIG. 9 shows the change in heavy metal contents at T0 and at T28 in the studied subjects.

[0103] FIG. 10 shows the change in MDA (FIG. 10A), catalase and SOD (FIG. 10B) contents, expressed as U/mg of proteins, at T0 and at T28 in subjects having received the placebo or having received the active agent.

[0104] FIG. 11 shows the change in carbonylated protein content at T0 and at T28 in subjects having received the placebo or having received the active agent. FIG. 11A is a bar chart and FIG. 11B shows photographs of cell layers with fluorescent staining of carbonylated proteins. Quantification is then performed by image analysis.

EXAMPLES

Example 1: Extract of the Invention

[0105] A polyphenolic extract is obtained according to the following method: [0106] a) preparation of a solution of oil cake of Passiflora edulis seeds, defatted by pressing and ground (10% dry matter), in a 70:30 (w/w) 1,3-propanediol/water mixture; [0107] b) extraction with stirring for 2 hours at 70? C.; [0108] c) removal of the residual plant by coarse filtration; and [0109] d) purification of the extract obtained by filtrations including an additional sterile filtration.

[0110] The liquid polyphenolic extract thus obtained has the following characteristics (% of dry extract): [0111] Dry extract (drying chamber): 0.9% (m/m) [0112] Total polyphenol content (by spectrophotometryin gallic acid equivalents): 43% [0113] Catechin derivative content (by HPLCin gallic acid equivalents): 22.3% [0114] Malic acid content (by enzymatic kit): 5.4% [0115] Citric acid content (by enzymatic kit): 3.7% [0116] Protein content (by Kjeldahl?6.25): 4.5%

Example 2: Extract of the Invention

[0117] A polyphenolic extract is obtained according to the following method: [0118] a) preparation of a solution of 13.3% Passiflora edulis seeds, non-defatted and ground, in a 70:30 1,3-propanediol/water mixture [0119] b) extraction with stirring for 2 hours at 70? C. [0120] c) removal of the residual plant by coarse filtration [0121] d) purification of the extract obtained by filtrations, including an additional sterile filtration.

[0122] The liquid polyphenolic extract thus obtained has the following characteristics (% of dry extract): [0123] Dry extract (drying chamber): 1.07% (m/m) [0124] Total polyphenol content (by spectrophotometrygallic acid equivalents): 40% [0125] Protein content (Kjeldahl?6.25): 5.7%

Example 3: Biological Activity of the Extract of the Invention

[0126] 1. Biological Potential

[0127] The biological potential of an extract of the invention was investigated using a gene expression modulation test on normal human fibroblasts (NHF) and on reconstructed and melanized human epidermises.

[0128] Thus, the expression of 46 genes involved in various physiological pathways of the epidermis (barrier, pigmentation, inflammation, etc.) and of the dermis (scarring, elasticity, firmness, etc.) was studied by PCR-array.

Materials and Methods:

[0129] Normal human fibroblasts (NHF) and melanized reconstructed epidermises were incubated for 24 hours in the presence of an extract of the invention, as obtained in Example 1, at 0.002% and 0.05% (w/v) for the NHF or at 0.002% and 0.005% (w/v) for the reconstructed epidermises, and in the presence of 20 ng/ml TGF-?1 on the NHF or in the presence of 1 nM vitamin D3 on the reconstructed epidermises (controls for validating the tests).

[0130] At the end of the treatment, RNA was extracted and gene expression was analysed by qRT-PCR using the TaqMan array targeting the key functions of the dermis and of the epidermis.

Results and Conclusion:

[0131] The results are presented in Tables 2 and 3 below and show in particular that the extract of the invention, while varying the gene expression of certain markers, is of particular interest in the following activities: [0132] the homeostasis and structure of the dermal extracellular matrix ( MMP3); and [0133] the dermo-epidermal junction ( LAMC2).

[0134] More particularly, the polyphenols of the extract of the invention enabled modulation of the expression of genes involved in antioxidant defences and the hormesis phenomenon ( HMOX1, FTL and G6PD).

[0135] This shows that the extract of the invention has antioxidant, antiradical and antiaging activity.

TABLE-US-00002 TABLE 2 Screening on NHF Extract of Extract of the invention the invention (0.002%) (0.05%) RQ* p-value RQ* p-value Haem oxygenase (HMOX1) 4.0641 0.0025 Ferritin, light polypeptide (FTL) 2.3263 0.0221 Glucose-6-phosphate 1.7197 0.0218 dehydrogenase (G6PD) Matrix metalloproteinase 1 0.6052 0.0282 (MMP1) Matrix metalloproteinase 3 0.5513 0.0173 (MMP3) *Level of gene expression expressed as relative quantity (RQ) in relation to the untreated control = 1

TABLE-US-00003 TABLE 3 Screening on reconstructed epidermises Extract of Extract of the invention the invention (0.002%) (0.05%) RQ* p-value RQ* p-value Laminin subunit gamma-2 2.2309 0.0387 4.1885 0.0165 (LAMC2) Proopiomelanocortin (POMC) 2.3447 0.0398 1.7019 0.0183 Melanocyte-stimulating hormone 0.7109 0.0175 0.6188 0.0127 receptor (MC1R) Cytosolic phospholipase A2 0.6829 0.0391 (PLA2G4A) L-Dopachrome tautomerase (DCT) 0.4637 0.0296 *Level of gene expression expressed as relative quantity (RQ) in relation to the untreated control = 1

[0136] 2. Activity on Induction of Hormesis and Cellular Detoxification

[0137] A hormetic molecule (or hormetin) is a substance having a biphasic effect, namely a beneficial effect at a low dose and the opposite effect at a high dose (e.g., prooxidant or antioxidant). A hormetin is also described as being a molecule which reproduces the effects of mild stress on the organism but which in return enables the cell to protect itself against future attacks and thus to protect the organism against various age-related diseases (cancers) or physical phenomena (skin ageing, poor healing, etc.) or harmful environmental effects (UV radiation, pollution, etc.).

[0138] The following analyses made it possible to study the activity on induction of hormesis and cellular detoxification of an extract of the invention.

[0139] AActivation of Translocation of Transcription Factor Nrf2:

[0140] The effect of the extract of the invention was evaluated with respect to activation of translocation of Nrf2, precursor of the cascade responsible for the hormetic response and for certain detoxification pathways of the organism.

Materials and Methods

[0141] ARE-luciferase-transfected HaCaT keratinocytes, containing the antioxidant response element (ARE) plasmid NQO1, which is a specific plasmid for activation of Nrf2 and luciferase (reporter gene), were treated for 6 hours at 37? C. with an extract of the invention, as obtained in Example 1, at concentrations ranging from 0.01% to 0.0005% (w/v) and with a positive reference, 20 ?M tert-butylhydroquinone.

[0142] At the end of the treatment, the cell monolayers are lysed, luciferase activity is assayed using a Luciferase Assay Kit from PROMEGA, and the protein content of each lysate is assayed using the Bradford method (BioRad).

Results and Conclusion

[0143] The results obtained are presented in Table 4 and in FIG. 1. These results show that the extract of the invention induced an increase in Nrf2 activity in the nuclei, and thus a translocation of Nrf2 across the nuclear membrane, showing activation of this transcription factor.

[0144] This shows that the extract of the invention has antioxidant, antiradical and antiaging activity.

TABLE-US-00004 TABLE 4 Nrf2 activity in ARE-luciferase HaCaT (RLU = relative luciferase activity) Nrf2 activity (mean normalized Activation RLU/?g of proteins) (%) Control 0 0 Tert-butylhydroquinone 23216.4 100 *** (20 ?M) Extract of the invention 4149.6 18 * (0.0005%) Extract of the invention 8178.2 35 *** (0.001%) Extract of the invention 9998.6 43 *** (0.005%) Extract of the invention 6324.6 27 *** (0.01%) * 0.01 < p < 0.05; ** 0.001 < p < 0.01; *** p < 0.001 and ns = not significant vs control cells - one-way ANOVA followed by Dunnett's test

[0145] BGene Expression of the Principal Markers of Hormesis and of Cellular Detoxification.

[0146] The effect of an extract of the invention was studied on the gene expression of various markers involved in the hormesis pathways and in cellular detoxification.

Materials and Methods:

[0147] Normal human fibroblasts were treated for 6 hours, 24 hours and 48 hours at 37? C. with a 0.005% and a 0.002% (w/v) extract of the invention, as obtained in Example 1.

[0148] At the end of the treatment, the gene expression of markers of hormesis (HMOX1, FTL, G6PD and Nrf2) and of markers involved in cellular detoxification (SOD1 and catalase) was analysed by quantitative real-time RT-PCR and normalized to the housekeeping gene HPRT (SybrGreen technology).

[0149] The results were statistically analysed by one-way ANOVA followed by Dunnett's test (GraphPad PRISM version 5.02 software, GraphPad Software, San Diego, Calif., USA).

Results:

[0150] The results obtained are presented in Table 5. These results show that the extract of the invention significantly stimulated the gene expression of HMOX1 at 6 hours and 24 hours, of FTL at three times, of G6PD at 6 hours and 24 hours, of SOD1 at 24 hours, of Nrf2 at 24 hours and 48 hours, and of catalase at 24 hours.

[0151] This shows that the extract of the invention has antioxidant, antiradical and antiaging activity.

TABLE-US-00005 TABLE 5 Gene expression of hormesis markers in normal human fibroblasts (relative quantity) HMOX1 FTL G6PD SOD1 Nrf2 Catalase 6 hours Control cells 1.00 1.00 1.00 1.00 1.00 1.00 Extract of the 3.38 0.94 1.31 1.03 1.00 0.97 invention (0.002%) (+238% ***) .sup.(?6% ns) (+31% *).sup. (+3% ns) .sup.(+0% ns) (?3% ns) Extract of the 23.06 1.37 1.31 1.05 1.04 1.02 invention (0.005%) (+2206% ***) (+37% ***) (+31% *).sup. (+5% ns) .sup.(+4% ns) (+2% ns) 24 hours Control cells 1.00 1.00 1.00 1.00 1.00 1.00 Extract of the 1.66 1.62 1.41 1.13 1.25 1.23 invention (0.002%) (+66% ***) (+62% **) (+41% ns) (+13% ns) (+25% *) (+23% ns) Extract of the 2.68 2.40 1.70 1.33 1.30 1.31 invention (0.005%) (+168% ***) (+140% ***) .sup.(+70% **) (+33% *).sup. (+30% *) (+31% *).sup. 48 hours Control cells 1.00 1.00 1.00 1.00 1.00 1.00 Extract of the 0.98 1.10 0.88 1.39 1.34 1.06 invention (0.002%) .sup.(?2% ns) (+10% ns).sup. (?12% ns) (+39% ns) (+34% *) (+6% ns) Extract of the 1.00 1.66 0.86 0.97 1.26 1.04 invention (0.005%) (0% ns) (+66% ***) (?14% ns) (?3% ns) .sup.(+26% ns) (+4% ns) * 0.01 < p < 0.05; ** 0.001 < p < 0.01; *** p < 0.001 and ns = not significant vs control cells - one-way ANOVA followed by Dunnett's test

[0152] CProduction of Haem Oxygenase:

[0153] An extract of the invention was analysed on the protein expression of haem oxygenase.

Materials and Methods:

[0154] Normal human fibroblasts were treated for 24 hours with a 0.002% and a 0.005% (w/v) extract of the invention, as obtained in Example 1, and with 5 and 10 ?M curcumin (reference hormetin).

[0155] At the end of the treatment, intracellular haem oxygenase 1 (or HMOX1 or HO1) was quantified using an ELISA technique. Staining, proportional to the quantity of the marker of interest, was measured by reading the optical density (OD) at 450 nm, and the value obtained was related to the quantity of cells obtained by a protein assay using the BC Assay (Interchim).

[0156] The results were statistically analysed by one-way ANOVA followed by Dunnett's test (GraphPad PRISM version 5.02 software, GraphPad Software, San Diego, Calif., USA).

Results:

[0157] The results obtained are presented in Table 6. These results show that the extract of the invention induces the production of haem oxygenase 1 with the same intensity as does curcumin. This result confirms the action of the extract of the invention which we observed during the study of gene expression of this same marker.

[0158] This shows that the extract of the invention has antioxidant, antiradical and antiaging activity.

TABLE-US-00006 TABLE 6 HMOX1 production by fibroblasts HMOX1 (ng/ml on quantity of cells) Inhibition Control cells 0.033 ? 0.003 Curcumin (5 ?M) 0.060 ? 0.002 82% *** Curcumin (10 ?M) 0.105 ? 0.006 218% *** Extract of the invention (0.002%) 0.056 ? 0.002 69% *** Extract of the invention (0.005%) 0.098 ? 0.004 197% *** *** p < 0.001 vs control cells - one-way ANOVA followed by Dunnett's test D- Production of haem oxygenase under Nrf2 siRNA conditions:

[0159] In order to verify whether the haem oxygenase activation pathway by an extract of the invention indeed passes through activation of Nrf2, the potential to induce production of haem oxygenase was verified in a system where Nrf2 expression is blocked (small interference RNA, or siRNA).

Materials and Methods:

[0160] Normal human fibroblasts were pretreated for 24 hours with Nrf2 siRNA and scrambled siRNA (control siRNA without action) and then, on each preceding condition, for 24 hours with a 0.005% (w/v) extract of the invention, as obtained in Example 1.

[0161] At the end of the treatment, intracellular haem oxygenase 1 (or HMOX1) was quantified using an ELISA technique. Staining, proportional to the quantity of the marker of interest, was measured by reading the optical density (OD) at 450 nm.

[0162] The results were statistically analysed by one-way ANOVA followed by Tukey's test (GraphPad PRISM version 5.02 software, GraphPad Software, San Diego, Calif., USA).

Results:

[0163] The results obtained are presented in Table 7. These results show that the stimulation of HMOX1 production induced by an extract of the invention is substantially decreased (?62%, p<0.001) when the Nrf2 pathway is partially blocked (Nrf2 siRNA).

[0164] The activity of an extract of the invention on the production of haem oxygenase thus indeed passes through the Nrf2 pathway.

[0165] This shows that the extract of the invention has antioxidant, antiradical and antiaging activity.

TABLE-US-00007 TABLE 7 HMOX1 production by fibroblasts - Comparative: scrambled siRNA vs Nrf2 siRNA HMOX1 Inhibition related (ng/ml) to Nrf2 siRNA Control cells - scrambled 3.371 ? 0.702 Control cells - Nrf2 siRNA 2.199 ? 0.089 ?35% * Extract of the invention (0.005%) - 18.821 ? 0.316 scrambled Extract of the invention (0.005%) - 7.105 ? 0.131 ?62% *** Nrf2 siRNA *** p < 0.001 vs control cells - one-way ANOVA followed by Tukey's test

[0166] EEffect on Production of Reactive Oxygen Species (ROS):

[0167] The antioxidant potential of an extract of the invention with respect to H.sub.2O.sub.2 induction of reactive oxygen species was studied.

Materials and Methods:

[0168] Normal human keratinocytes were incubated for 24 hours in the presence of a 0.002%, a 0.005% and a 0.01% (w/v) extract of the invention, as obtained in Example 1, or of 500 ?M vitamin C and 10 ?M quercetin (reference antioxidants) before incorporation of the H2DCF-DA probe (incubation for 60 minutes).

[0169] The keratinocytes were then stimulated with 100 ?M hydrogen peroxide (H.sub.2O.sub.2) for 20 minutes and the production of reactive oxygen species (ROS) was evaluated by measurement of fluorescence.

[0170] The results were statistically analysed by one-way ANOVA followed by Tukey's test (GraphPad PRISM version 5.02 software, GraphPad Software, San Diego, Calif., USA).

Results:

[0171] The results obtained are presented in Table 8. These results show that the extract of the invention significantly inhibited the production of ROS by keratinocytes in response to hydrogen peroxide (H.sub.2O.sub.2)-induced oxidant stress. The level of this antioxidant activity is equivalent to that of the two control antioxidants (vitamin C and quercetin).

[0172] This shows that the extract of the invention has antioxidant, antiradical and antiaging activity.

TABLE-US-00008 TABLE 8 ROS production in H.sub.2O.sub.2-treated keratinocytes ROS (fluorescence units) Significance Control cells 30752.921 ? 4555.136 Stimulated cells (H.sub.2O.sub.2) 44179.976 ? 7445.110 +44% *** Reference (vitamin C) 21719.018 ? 3174.253 ?51% *** Reference (quercetin) 14367.847 ? 1790.753 ?67% *** Extract of the invention (0.002%) 18769.676 ? 2780.206 ?58% *** Extract of the invention (0.005%) 14404.891 ? 2974.938 ?67% *** Extract of the invention (0.01%) 16531.685 ? 1902.378 ?63% *** *** p < 0.001 - one-way ANOVA followed by Tukey's test

[0173] 3. Protection Against the Harmful Effects of Pollution

[0174] The preceding results showed that an extract of the invention stimulates the production of haem oxygenase 1 via activation of translocation of transcription factor Nrf2. Consequently, the extract of the invention enabled antioxidant cellular protection via reduction of ROS formation induced by H.sub.2O.sub.2 stress.

[0175] The extract of the invention making it possible to stimulate skin defences, we evaluated their protective effect with respect to various environmental stresses, in this case pollution.

[0176] AEffect on Oxidant Stress:

Materials and Methods:

[0177] Normal human keratinocytes were treated for 24 hours with a 0.002% (w/v) extract of the invention, as obtained in Example 1, 500 ?M vitamin C and 10 ?M quercetin (reference antioxidants), with 10 ?M curcumin (reference hormetin) or with 10 ?M resveratrol before incorporation of the H2DCF-DA probe (incubation for 45 minutes). The keratinocytes were then stimulated with 9 ?g/ml benzo-?-pyrene (BaP) for 20 minutes.

[0178] ROS production was evaluated by measurement of fluorescence.

[0179] The significance of the results was verified by Student's t-test.

Results and Conclusion:

[0180] The results obtained are presented in Table 9. These results show that the 0.002% extract of the invention inhibited ROS production by keratinocytes in response to oxidant stress induced by 6 and 9 ?g/ml BaP.

[0181] Therefore, the extract of the invention exerts a protective effect with respect to pollution-induced oxidant stress. The extract of the invention thus has antioxidant, antiradical, antipollution and antiaging activity.

TABLE-US-00009 TABLE 9 ROS production in keratinocytes treated with 9 ?g/ml BaP ROS (fluorescence units) Significance Control cells 4730 ? 324 Stimulated cells 16777 ? 1755 +255% *** (9 ?g/ml BaP) Reference (vitamin C) 14089 ? 1719 ?16% NS Reference (quercetin) 17140 ? 930 ?32% NS 10 ?M curcumin 14065 ? 1231 ?16% NS 10 ?M resveratrol 20357 ? 503 ?21% * Extract of the invention 11052 ? 828 34% ** (0.002%) * p < 0.05; ** p < 0.01; *** p < 0.001 and ns = not significant - Student's t-test

[0182] BProtection of Skin Structures:

[0183] The ability of an extract of the invention to protect the integrity of the skin (dermis and epidermis) from the harmful effects of pollution was studied on human skin explants.

Materials and Methods:

[0184] Human skin explants from a 45-year-old woman were pretreated for 24 hours with a topical application of a cosmetic formula containing or not containing (placebo) 3% extract of the invention, as obtained in Example 1.

[0185] Said explants were then treated again with the cosmetic formulas in the presence of benzo-?-pyrene (BaP, 20 ?M) for Protocol 1 or of Bap+nicotine (20 ?M) for Protocol 2.

[0186] Immunostainings of various skin structure markers were performed.

Results and Conclusion:

[0187] The results obtained are presented in FIGS. 2 to 7.

[0188] The stress mimicking pollution induced by BaP?nicotine led to altered expression of the structural markers studied.

[0189] Under these conditions, the extract of the invention protected the following epidermal markers: [0190] Claudin 4 (marker of the tight junctions/barrier function), [0191] Filaggrin (marker of the barrier function and precursor of natural hydration factors), and [0192] Loricrin (marker involved in cell differentiation/barrier function);
And the dermal markers: [0193] Collagen I (marker involved in skin firmness), [0194] Elastin (marker involved in skin elasticity), and [0195] Fibronectin (marker involved in dermal structure).

[0196] These results show a real protective effect of the structural integrity of the epidermis and of its barrier function as well as the maintenance of a normal structure of the dermis and therefore an overall skin protective effect against environmental pollution.

[0197] The extract of the invention thus has antioxidant, antiradical, antipollution and antiaging activity.

[0198] CEvaluation of Detoxifying Activity with Respect to Ozone Stress

[0199] The protective and detoxifying potentials of an extract of the invention with respect to ozone stress were evaluated on reconstructed epidermises.

Materials and Methods:

[0200] Reconstructed human epidermises (RHE) were pretreated for 24 hours with a topical application of a cosmetic formula containing or not containing (placebo) 3% extract of the invention, as obtained in Example 1.

[0201] The epidermises were then subjected to stress with 0.5 to 1 ppm ozone.

[0202] The enzymatic activities of the detoxifying enzymes catalase, glutathione peroxidase (GPX) and superoxide dismutase (SOD) were quantified by a calorimetric (SOD and GPX) or fluorescence (catalase) ELISA method.

[0203] Lipid peroxidation was evaluated by the malondialdehyde (MDA) assay, performed by GC.

[0204] DNA oxidation was evaluated by the 8-hydroxydeoxyguanosine (8-OHdG) assay, performed by colorimetry.

[0205] The results were statistically analysed by one-way ANOVA followed by Tukey's test (GraphPad PRISM version 5.02 software, GraphPad Software, San Diego, Calif., USA).

Results and Conclusion:

[0206] Ozone stress induced overactivation of catalase, SOD and GPX enzymes; this increase attests to the ozone-induced oxidant stress.

[0207] Under these conditions, the extract of the invention significantly inhibited this overactivation.

[0208] In the absence of ozone stress (basal condition), the extract of the invention induces no inhibition of the activity of the detoxifying enzymes. This confirms that it is indeed a protective effect with respect to the induced stress and not an inhibition of the detoxification systems of the cell.

(Tables 10, 11 and 12).

[0209] Ozone stress induced oxidative damage expressed as an increase in 8-OHdG and in MDA.

[0210] The extract of the invention significantly protects cell components from the oxidative damage induced by ozone stress by significantly inhibiting the production of 8-OHdG and of MDA.

(Tables 13 and 14).

[0211]

TABLE-US-00010 TABLE 10 Catalase activity in RHE optionally treated with ozone Mean % change Tukey Catalase assay under ozone stress conditions Control epidermises 156.1 0 Ozone 340.1 118 $$$ Placebo 306.4 ?10 ns Extract of the invention (3%) 244.2 ?28 *** Catalase assay under basal conditions Control 181.8 0 Placebo 250.3 38 $$$ Extract of the invention (3%) 231.4 27 $$ $$ 0.001 < p < 0.01; $$$ p < 0.001 vs control ** 0.001 < p < 0.01; *** p < 0.001 vs ozone

TABLE-US-00011 TABLE 11 GPX activity in RHE optionally treated with ozone Mean % change Tukey GPX assay under ozone stress conditions Control 13.0 0 Ozone 34.9 168 $$$ Placebo 31.1 ?11 * Extract of the invention (3%) 23.3 ?33 *** GPX assay under basal conditions Control 13.0 0 Placebo 12.3 ?6 ns Extract of the invention (3%) 13.2 2 ns $$$ p < 0.001 vs control- * 0.01 < p < 0.05; *** p < 0.001 vs ozone

TABLE-US-00012 TABLE 12 SOD activity in RHE optionally treated with ozone Mean % change Tukey SOD assay under ozone stress conditions Control 5.7 0 Ozone 13.0 130 $$$ Placebo 9.5 ?27 *** Extract of the invention (3%) 7.2 ?45 *** SOD assay under basal conditions Control - 4.7 0 Placebo formula 5.4 15 $$ Extract of the invention (3%) 4.9 4 ns $ 0.01 < p < 0.05; $$ 0.001 < p < 0.01 $$$ p < 0.001 vs control ** 0.001 < p < 0.01; *** p < 0.001 vs ozone

TABLE-US-00013 TABLE 13 8-Oxo-dG assay in ozone-treated RHE Mean % change Tukey Control 2.9 0 Ozone 7.4 155 $$$ Placebo 5.4 ?27 ** Extract of the invention (3%) 3.7 ?51 *** $$$ p < 0.001 vs control * 0.01 < p < 0.05; ** 0.001 < p < 0.01; *** p < 0.001 vs ozone

TABLE-US-00014 TABLE 14 MDA assay in ozone-treated RHE Mean % change Tukey Control 96.6 0 Ozone 251.4 160 $$$ Placebo 204.0 ?19 ** Extract of the invention (3%) 176.6 ?30 *** $$$ p < 0.001 vs control ** 0.001 < p < 0.01; *** p < 0.001 vs ozone

[0212] 4. Protection Against Sun-Related Deleterious Effects

[0213] Ultraviolet (UV) and infrared (IR) rays penetrate the skin to varying depths and are responsible for, amongst other things, a decrease in skin firmness and an increase in the quantity of free radicals released, thus leading to premature skin ageing. Such rays are also responsible for the formation of melanomas and for skin immunosuppression. The protective effect of an extract of the invention with respect to UV- or IR-induced stress was studied.

[0214] AProtection Against UV-Induced DNA Damage (Comet Assay):

[0215] Maintenance of the nuclear integrity of the cell, with respect to UV radiation, was tested using the comet assay.

Materials and Methods:

[0216] Normal human keratinocytes were treated for 2 hours with a 0.001% (w/v) extract of the invention, as obtained in Example 1.

[0217] A comet assay is performed according to the method described by Singh et al. in 1988 and by Meo et al. in 1991, and which consists in irradiating cells with light at 4.5 J/cm.sup.2 (0.28 J/cm.sup.2 UVA; 0.08 J/cm.sup.2 UVB and 4.14 J/cm.sup.2 visible light) corresponding to 1 to 3 minutes of sun exposure in mid-summer. This is followed by DNA migration using agarose gel electrophoresis.

[0218] A relative value ?.sup.2 OTM is calculated using the Systat software, this value being directly proportional to the size of the comet and thus to the degree of protection of the active agent with respect to UV radiation.

[0219] The significance of the results was verified by analysis of variance using the SigmaPlot software (version 11.0, Systat Software, Chicago, Ill., USA).

Results and Conclusion:

[0220] The results are presented in Table 15 and in FIG. 8.

[0221] These results show that the 0.001% extract of the invention protected keratinocytes with respect to UV-irradiation-induced DNA damage (54% protection, p<0.001).

[0222] The extract of the invention thus has antioxidant, antiradical, antipollution and antiaging activity.

TABLE-US-00015 TABLE 15 Percent protection of cells with respect to UV radiation ?.sup.2 OTM % protection Control without UV 2.09 ? 0.1 100 Control irradiated at 4.5 J/cm.sup.2 11.59 ? 0.41 0 *** Extract of the invention (0.001%) 6.43 ? 0.28 54.4 *** *** p < 0.001 vs non-irradiated cells and vs cells irradiated at 4.4 J/cm.sup.2. Statistics from SigmaPlot

[0223] BInhibition of IR-Induced Production of MMP1

[0224] Infrared (IR) rays, which represents more half of the solar spectrum, can induce degradation of the dermal matrix, contributing to skin ageing, by stimulating the production of proteases such as MMP1.

[0225] The ability of an extract of the invention to protect dermal cells from the harmful effects of IR rays was studied by evaluating MMP1 production.

Materials and Methods:

[0226] Normal human fibroblasts (NHF) were incubated in the presence of a 0.001% extract of the invention, as obtained in Example 1, or of 10.sup.?7 M dexamethasone (reference anti-inflammatory) for 48 hours following 1 hour of infrared irradiation (0.57 kJ/cm.sup.2).

[0227] After incubation, the supernatants are collected in order to assay released MMP1 (ELISA Kit, R&D Systems).

[0228] The significance of the results was verified by Student's t-test.

Results and Conclusion:

[0229] The results are presented in Table 16. These results show that the 0.001% extract of the invention significantly inhibited infrared-induced production of MMP1 in normal human fibroblasts.

[0230] The extract of the invention thus has antioxidant, antiradical, antipollution and antiaging activity.

TABLE-US-00016 TABLE 16 IR-induced production of MMP1 in normal human fibroblasts MMP1 (ng/ml) % protection Control without IR 11 100 *** Control irradiated at 0.57 kJ/cm.sup.2 88.6 0 Dexamethasone (10.sup.?7 M) 9.3 102 *** Extract of the invention (0.001%) 77.4 14 * * p < 0.05; *** p < 0.001 vs irradiated cells

[0231] CProtection Against UV-Induced Oxidant Stress:

[0232] The antioxidant potential of an extract of the invention with respect to UV-irradiation-induced reactive oxygen species (ROS) was studied.

Materials and Methods:

[0233] Normal human keratinocytes were incubated for 24 hours in the presence of a 0.001% and a 0.005% (w/v) extract of the invention, as obtained in Example 1, or of 500 ?M vitamin C (reference antioxidant), before incorporation of the H2DCF-DA probe (incubation for 1 hour).

[0234] The keratinocytes were then stimulated with UV at 2400 J/m.sup.2 (2000 J/m.sup.2 UVB and 400 J/m.sup.2 UVA) then returned to culture in the presence of a 0.001% and a 0.005% (w/v) extract of the invention, as obtained in Example 1, or of 500 ?M vitamin C, for 15 minutes at 37? C.

[0235] The production of reactive oxygen species (ROS) was evaluated by measurement of fluorescence and the value obtained was normalized to the quantity of cells obtained using an MTT cell viability assay.

[0236] The results were statistically analysed by one-way ANOVA followed by Tukey's test (GraphPad PRISM version 5.02 software, GraphPad Software, San Diego, Calif., USA).

Results and Conclusion:

[0237] The extract of the invention significantly inhibited ROS production by keratinocytes in response to UV-induced (2400 J/m.sup.2) oxidant stress (Table 17).

TABLE-US-00017 TABLE 17 ROS production in keratinocytes treated by UV at 2400 J/m.sup.2 ROS (fluorescence Signifi- intensity/MTT) % change cance Control cells 30936 ? 4925 Irradiated cells (UV 2400 J/m.sup.2) 43038 ? 5735 39 $$$ Reference (vitamin C) 26005 ? 3723 ?40 *** Extract of the invention (0.001%) 22564 ? 3849 ?48 *** Extract of the invention (0.005%) 19614 ? 4576 ?54 *** $$$ p < 0.001 vs control without UVs *** p < 0.001 vs control with UVs One-way ANOVA followed by Tukey's test

[0238] 5. Protection Against the Effects of Chemical Stress

[0239] Effect on PMA-Induced Production of PGE2:

[0240] The anti-inflammatory protection of an extract of the invention with respect to a chemical molecule, PMA, was studied by analysis of the release of prostaglandin 2 (PGE2).

Materials and Methods:

[0241] Normal human keratinocytes were pretreated with a 0.002% and a 0.005% extract of the invention, as obtained in Example 1, and with 10.sup.?6 M indomethacin (anti-inflammatory control of the prostaglandin pathway) for 24 hours at 37? C., in order to be able to measure a level of protection of the active agent with respect to inflammation by 0.1 ?g/ml PMA (phorbol 12-myristate 13-acetate) used on these same cell monolayers for a further 24 hours.

[0242] At the end of the treatment, the supernatants are collected and an assay of prostaglandin E2 (PGE2; R&D Systems) is performed. Staining, proportional to the amount of PGE2, was measured by reading the optical density (OD) at 450 nm.

[0243] The significance of the results was verified by Student's t-test.

Results:

[0244] The results are presented in Table 18. These results show that the extract of the invention at both concentrations significantly decreased PGE2 release with respect to induction by 0.1 ?g/mlPMA.

[0245] The extract of the invention thus has antioxidant, antiradical, antipollution and antiaging activity.

TABLE-US-00018 TABLE 18 PGE2 production in PMA-treated keratinocytes PGE2 (pg/ml) Inhibition Control cells 39 ? 0 100% *** PMA (0.1 ?g/ml) 113806 ? 11441 0% Indomethacin (10.sup.?6 M) 52 ? 8 100% *** Extract of the invention 35750 ? 2192 69% ** (0.002%) Extract of the invention 30382 ? 2401 73% ** (0.005%) ** 0.001 < p < 0.01 and *** p < 0.001 vs PMA-stimulated cells Student's t-test

[0246] 6. Protection Against the Effects of Ageing

[0247] The repeated action of environmental stresses such as pollution, the harmful effects of the sun, chemical molecules and all other forms of induction of oxidant stress, lead to degradation of the dermal matrix and thus to premature skin ageing.

[0248] An extract of the invention was analysed on a model of cell ageing in order to analyse their actions with respect to proteins which are underexpressed or overexpressed with age.

Materials and Methods:

[0249] Keratinocytes are cultured, and trypsinized each week, for 4 weeks in culture medium inducing an ageing phenotype (pro-age medium) optionally in the presence of a 0.000025% DM extract of the invention, as obtained in Example 1.

[0250] At the end of 3 weeks of culture/passages, a proteomic analysis is performed. The analysis grouped the proteins of the various cellular pathways into six domains: metabolism, apoptosis, detoxification, protein catabolism and protein synthesis.

Results and Conclusion:

[0251] The results are presented in Table 19. In this context of induction of ageing, the extract of the invention stimulated and/or protected the expression of several proteins involved mainly in cellular detoxification/protection and immune defences:

Cellular Detoxification and Protection:

[0252] Stimulation of peroxiredoxin 2 (PRDX2): an antioxidant enzyme playing a role in cellular protection against damage caused by intracellular ROS. [0253] Stimulation of carbonyl reductase 1 (CBR1): a dehydrogenase/reductase which reduces carbonyl compounds (medicinal products) and intervenes in detoxification during lipid peroxidation. The latter is inhibited under the pro-age conditions and is stimulated by the extract of the invention under these conditions. [0254] Inhibition of aldehyde dehydrogenase 2 (ALDH2): a mitochondrial enzyme playing a role in cell protection and differentiation which catalyses/detoxifies aldehyde/carbonyl molecules (medicinal products, pollution, etc.). This protein is stimulated under the pro-age conditions, and the extract of the invention restores its expression to a basal level. [0255] Stimulation of fatty acid-binding protein 5 (FABP5): a chaperone protein mainly expressed in the epidermis and involved in regulation of lipid homeostasis and thus playing a role in the barrier function. The pro-age conditions inhibit its expression, which is restored by passion fruit polyphenols. [0256] Stimulation of proteasome ?2 and ?6 subunits (PSMB2 and PSMB6): Proteasomes are involved in the removal of damaged/oxidized proteins and in the replacement of intracellular proteins. The proteasome consists of a and ? subunits which, amongst other things, cleave damaged proteins on the level of glutamine (?6) and on the level of trypsin (?2). Age decreases proteasome activity, which leads to an accumulation of damaged/oxidized proteins; this age effect is found under the pro-age conditions, and under these conditions the extract of the invention stimulates expression of these two proteasome subunits.

Immune Defence:

[0257] Inhibition of beta-2-microglobulin (B2MG): a small surface protein (epidermis) involved in the immune response. It is part of the major histocompatibility complex and is overexpressed in disease conditions, thus producing interleukins, notably 6 and 8, as well as 10, which is an immunosuppressive interleukin. Here, under conditions inducing ageing, its expression is increased. The extract of the invention prevents this increase. [0258] Inhibition of lectin, mannose-binding, 1 (LMAN1): a protein participating in the immune response by enabling phagocytosis of apoptotic cells and of pathogens. A deficiency in this gene entails an increase in cell debris in the skin. It is scarce, if present at all, in the basal state and is increased in inflammatory conditions (e.g., UV). Under the pro-age conditions, its expression is increased and modulated under the action of the extract of the invention.

TABLE-US-00019 TABLE 19 Protein expression (in %) of markers involved in detoxification and in immunity % induction with respect % induction with respect to normal medium to pro-age medium Extract of Extract of Pro-age the invention Pro-age the invention Proteins medium (0.000025%) medium (0.000025%) Peroxiredoxin 2 11.46 16.68 0.00 45.51 (PRDX2) Carbonyl reductase ?15.87 ?7.46 0.00 52.99 1 (CBR1) Aldehyde dehydro- 46.81 4.97 0.00 ?89.38 genase 2 (ALDH2) Fatty acid-binding ?24.82 4.68 0.00 118.87 protein 5 (FABP5) Proteasome ?2 ?21.15 ?10.96 0.00 48.21 (PSMB2) Proteasome ?6 ?16.85 ?10.92 0.00 35.21 (PSMB6) Beta-2-micro- 31.16 ?0.57 0.00 ?101.83 globulin (B2MG) Lectin, mannose- 40.13 3.71 0.00 ?90.75 binding, 1 (LMAN1)

[0259] 7. Conclusion

[0260] These various tests show an anti-inflammatory, antioxidant, antipollution and thus antiaging effect of the polyphenolic extract of the invention.

Example 4: Evaluation of In Vivo Efficacy of the Passion Flower Polyphenol Active Agent Versus Placebo by Measurement of the Amount of MDA, SOD, Catalase (CAT) and Carbonylated Proteins

Study Design:

[0261] Double-blind study. [0262] Comparative, randomized study

Population:

[0263] Two groups of 30 Asian female subjects (1 active agent group and 1 placebo group), between 30 and 50 years of age, of all skin types, living in a polluted environment.

Use:

[0264] The products are applied to one side of the face, by the subjects themselves, at home, twice per day (morning and evening) for 28 days.

Study Protocol:

[0265]

TABLE-US-00020 TABLE 20 Protocol of the clinical study T 28 Evaluation T0 days Hair sampling X X Biological sampling of the cheeks for MDA, CAT, SOD X X Biological sampling of the cheeks for carbonylated proteins X X

Mean Real Age Per Panel:

[0266] Panel total: 39.9 years (between 30 and 50 years)

Composition Administered:

Placebo Formula

[0267]

TABLE-US-00021 % Material name INCI EU material PURIFIED WATER R&D AQUA 75.12 SODIUM EDETATE DISODIUM EDTA 0.10 BUTYLENE GLYCOL BUTYLENE GLYCOL 3.00 CARBOPOL ULTREZ 10 CARBOMER 0.60 OCTANEDIOL XI CAPRYLYL GLYCOL 0.30 EMULIUM DELTA CETYL ALCOHOL 3.50 GLYCERYL STEARATE PEG-75 STEARATE CETETH-20 STEARETH-20 ISONONYL ISONONYL ISONONANOATE 10.00 ISONONANOATE PURE CETYL ALCOHOL CETYL ALCOHOL 0.50 CAPRYLOYL GLYCINE CAPRYLOYL GLYCINE 0.80 SODIUM HYDROXIDE XI AQUA 1.08 SODIUM HYDROXIDE PURIFIED WATER R&D AQUA 2.00 PURIFIED WATER R&D AQUA 3.00 100.00

Active Formula

[0268]

TABLE-US-00022 % Material name INCI EU material PLACEBO 97 PASSION FRUIT POLYPHENOLS AQUA 3 1 KG PROPANEDIOL PASSIFLORA EDULIS FRUIT EXTRACT 100

[0269] 1. Evaluation of the Pollution Received by the Subject at T0 and T28

[0270] At T0 and T28, a 1-cm lock of hair was taken from the scalp. A biochemical analysis was performed in order to determine the heavy-metal exposure of the subjects at each time point. The analysis involved 10 subjects, 5 from each panel.

[0271] The results (Table 21 and FIG. 9) show a constant amount of heavy metals or a slight increase. It can be concluded that the subjects' exposure to pollution did not decrease during the study, ruling out the hypothesis of a beneficial effect due only to a variation in pollution.

TABLE-US-00023 TABLE 21 Heavy metal contents Pb Fe Ni Cu Cd (ng/g) (ng/g) (ng/g) (ng/g) (ng/g) T0 218.5 7603.9 249.7 9283.8 25.5 T28 366 9487 328 10711 37 Significant Yes Yes No No Yes difference p = p = p = p = p = 0.0098 0.0488 0.0840 0.2324 0.0019

[0272] 2. Evaluation of the Amount of MDA, CAT and SOD at T0 and T28

[0273] At T0 and T28, a swab was taken from each cheek of the subjects. A biochemical analysis was performed in order to determine the amount of MDA, CAT and SOD. The analysis involved 30 subjects, 15 from each panel.

[0274] The amount of MDA, CAT and SOD significantly decreases between T0 and T28 for the active agent and the placebo, with a significantly larger decrease for the active agent (Table 22 and FIGS. 10A and 10B).

TABLE-US-00024 TABLE 22 MDA, CAT and SOD contents at T0 and T28 MDA CAT SOD Placebo Active agent Placebo Active agent Placebo Active agent T0 244.0 ? 77.9 244.0 ? 77.9 34.1 ? 4.98 34.1 ? 4.98 30.6 ? 5.0 30.6 ? 5.0 T28 213.7 ? 65.1 185.7 ? 56.3 28.9 ? 4.7 27.8 ? 4.6 25.8 ? 3.4 24.8 ? 3.7 % change ?12.4% ?23.9% ?15.4% ?18.6% ?15.9% ?18.9% Significance Yes Yes Yes Yes Yes Yes T28 ? T0 p < 0.01 p < 0.01 p < 0.01 p < 0.01 p < 0.01 p < 0.01 Significance Yes Yes Yes Placebo vs p < 0.01 p < 0.01 p < 0.01 Active agent

[0275] 3. Evaluation of the Amount of Carbonylated Proteins at T0 and T28

[0276] At T0 and T28, D-Squame sampling was performed on each cheek of the subjects. Biochemical staining was performed in order to determine the amount of carbonylated proteins. The analysis involved 20 subjects, 10 from each panel.

[0277] The amount of carbonylated proteins significantly decreases between T0 and T28 for the active agent and the placebo, with a significantly larger decrease for the active agent (Table 23 and FIGS. 11A and 11B).

TABLE-US-00025 TABLE 23 Carbonylated protein contents at T0 and T28 Carbonylated proteins Placebo Active agent T0 17.6 ? 5.6 17.6 ? 5.6 T28 12.6 ? 7.9 8.5 ? 5.5 % change ?28.1% ?51.5% Significance T28 ? T0 Yes Yes p < 0.01 p < 0.01 Significance Placebo vs Yes Active agent p = 0.01

[0278] 4. Summary of Biochemical Measurements

[0279] The results show an antiradical/detoxifying efficacy both on the overall panel and on the non-smoker and smoker subpanels.

[0280] The active agent substitutes for the skin's natural defences, SOD and CAT, thus leading to a smaller amount of residues resulting from the detoxification process (MDA). The results for carbonylated proteins confirm this antiradical/detoxifying action.

[0281] 5. Summary of Efficacy

[0282] The active agent (3%) showed a significant efficacy on the following parameters: [0283] Amount of MDA in the cheek

[0284] The active agent induced the following effects on the amount of MDA measured from the biological sample of the cheek: [0285] For the overall panel (30 subjects), a significant decrease of 23.9%. The effect observed is significant relative to the effect observed with the placebo. [0286] Amount of SOD in the cheek

[0287] The active agent induced the following effects on the amount of SOD measured from the biological sample of the cheek: [0288] For the overall panel (30 subjects), a significant decrease of 18.6%. The effect observed is significant relative to the effect observed with the placebo. [0289] Amount of CAT in the cheek

[0290] The active agent induced the following effects on the amount of CAT measured from the biological sample of the cheek: [0291] For the overall panel (30 subjects), a significant decrease of 18.9%. The effect observed is significant relative to the effect observed with the placebo. [0292] Amount of carbonylated proteins in the cheek

[0293] The active agent induced the following effects on the amount of carbonylated proteins measured from the biological sample of the cheek: [0294] For the overall panel (20 subjects), a significant decrease of 51.5%. The effect observed is significant relative to the effect observed with the placebo. [0295] .fwdarw.All of these results show significant effects in terms of detoxification.

Example 5: Compositions for Application Via the Topical Route

[0296] Several compositions for application via the topical route are presented below. The polyphenolic extract of passion flower seeds, of Example 1 or 2, can be incorporated in various cosmetic products, such as cleansing waters, oil-in-water emulsions, water-in-oil emulsions, oils, milks, lotions, shampoos, foaming products and sprays, the compositions of which are presented below by way of example.

TABLE-US-00026 SENSITIVE SKIN CLEANSING WATER Raw material/Brand name or INCI name % CAPRYLOYL GLYCINE From 0 to 1% SODIUM HYDROXIDE From 0 to 1% SEQUESTRANT From 0 to 1% BUTYLENE GLYCOL From 1 to 5% BETA CAROTENE From 0 to 2% POLYPHENOLIC EXTRACT OF From 0.001 to 10% PASSION FLOWER PRESERVATIVES From 0 to 1% PEG-32 From 1 to 5% PEG-7 PALM COCOATE From 1 to 5% ZINC GLUCONATE From 0 to 1% CITRIC ACID From 0 to 1% PURIFIED WATER QS 100% FRAGRANCE From 0 to 1% POLOXAMER 184 From 1 to 5%

TABLE-US-00027 ANTI-AGE EMULSION Raw material/Brand name or INCI name % LIQUID ISOPARAFFIN From 5 to 20% ISOCETYL STEARATE From 5 to 20% ALMG HYDROXY STEARATE From 5 to 20% ABIL WE 09 From 1 to 5% GLYCEROL From 1 to 5% VASELINE OIL From 1 to 5% MICRONIZED ZINC OXIDE From 1 to 5% BUTYLENE GLYCOL From 1 to 5% RETINOL From 0 to 1% VITAMIN C From 0 to 5% POLYPHENOLIC EXTRACT OF From 0.01 to 10% PASSION FLOWER ISONONYL ISONONANOATE From 1 to 5% BEESWAX From 1 to 5% SODIUM TARTRATE From 1 to 5% SODIUM CHLORIDE From 0 to 5% GLYCINE From 1 to 5% PRESERVATIVES From 0 to 1% CHOLESTEROL From 0 to 1% PHYTOSPHINGOSINE From 0 to 1% TARTARIC ACID From 0 to 1% PURIFIED WATER QS 100%