Heterologous pathway to produce terpenes

Abstract

Cells comprising a heterologous metabolic pathway are configured to produce a terpene product containing non-multiples of five carbon, particularly wherein the pathway comprises heterologous Lepidoptera insect juvenile hormone biosynthetic pathway enzymes of the insect's mevalonate pathway.

Claims

1. A cell genetically engineered to express a heterologous metabolic pathway comprising heterologous Bombyx mori and/or Choristoneura fumiferana mevalonate pathway enzymes, wherein the cell produces a product of the metabolic pathway that is a C16 terpene, wherein the mevalonate pathway enzymes comprise -hydroxy -methylglutaryl-CoA synthase (HMGS), -hydroxy -methylglutaryl-CoA reductase (HMGR), mevalonate kinase (MevK), mevalonate phosphate kinase (MevPK), mevalonate pyrophosphate decarboxylase (MevPPD), isopentenyl pyrophosphate isomerase (IPPI) and farnesyl pyrophosphate synthase (FPPS), wherein the metabolic pathway further comprises a terpene cyclase.

2. The cell of claim 1, wherein the terpene cyclase is Streptomyces coelicolor epi-isozizaene synthase.

3. The cell of claim 1, wherein the metabolic pathway further comprises propionate-CoA ligase.

4. The cell of claim 1, wherein the metabolic pathway further comprises S. sativa valeryl-CoA synthase, E. coli FadB and/or Micrococcus luteus Aco.

5. The cell of claim 1 that is E. coli.

6. A method for producing a C16 terpene product, wherein said method comprises growing the cell of claim 1 to produce the product, and isolating or enriching the product.

7. A method of making the cell of claim 1, wherein said method comprises genetically engineering a cell to comprise a heterologous metabolic pathway comprising a terpene cyclase and heterologous Bombyx mori and/or Choristoneura fumiferana mevalonate pathway enzymes, wherein the mevalonate pathway enzymes comprise -hydroxy -methylglutaryl-CoA synthase (HMGS), -hydroxy -methylglutaryl-CoA reductase (HMGR), mevalonate kinase (MevK), mevalonate phosphate kinase (MevPK), mevalonate pyrophosphate decarboxylase (MevPPD), isopentenyl pyrophosphate isomerase (IPPI) and farnesyl pyrophosphate synthase (FPPS), and wherein the cell produces a product of the metabolic pathway that is a C16 terpene.

Description

BRIEF DESCRIPTION OF THE FIGURES

(1) FIG. 1. Canonical mevalonate pathway

(2) FIG. 2. Lepidopteran mevalonate pathway

(3) FIG. 3. GC Single Ion Chromatogram 218 Corresponding to C16 Terpene; inset: Enriched Sample Shows C16 Terpene Consistent Fragmentation Pattern

(4) FIG. 4. Several Juvenile hormones found in nature, and intermediates

(5) FIG. 5. Alternative Starting Units

(6) FIGS. 6, 7. Pathway Expression Vectors

(7) FIG. 8. Alternative R groups

DESCRIPTION OF PARTICULAR EMBODIMENTS OF THE INVENTION

(8) Unless contraindicated or noted otherwise, in these descriptions and throughout this specification, the terms a and an mean one or more, the term or means and/or and polynucleotide sequences are understood to encompass opposite strands as well as alternative backbones described herein.

(9) It is understood that the examples and embodiments described herein are for illustrative purposes only and that various modifications or changes in light thereof will be suggested to persons skilled in the art and are to be included within the spirit and purview of this application and scope of the appended claims. All publications, patents, and patent applications cited herein, including citations therein, are hereby incorporated by reference in their entirety for all purposes.

(10) Canonical Mevalonate Pathway:

(11) The canonical mevalonate pathway starts with the condensation of two acetyl-CoAs producing acetoacetyl-CoA via a thiolase. Another acetyl-CoA is then condensed making HMG-CoA via HMG-CoA synthase. HMG-CoA undergoes a double reduction by the cofactor NadPH with the enzyme HMG-CoA reductase producing mevalonate. An embodiment can also employ a engineered lepidoptera HMGR that accepts NADH instead of NADPH (such HMGRs are found in nature, though not from lepidotera) in a pathway to make homoterpenes. A phosphate moiety is then added by MevK making MevP. MevP has another phosphate added by the enzyme MevPK making MevPP (at the expense of ATP). MevPP undergoes a decarboxylation catalyzed by the enzyme MevPP decarboxylase, at the expense of an ATP, producing IPP. IPP can be isomerized by IPP isomerase making DMAPP. The prenyltransferase FPP synthase then condenses one IPP and one DMAPP to make GPP. The same enzyme then takes GPP and IPP to produce FPP. FPP is an essential metabolite used to make a large number of compounds including quinones, cell wall components, and sesquiterpenes secondary metabolites among others. FIG. 1.

(12) Lepidoptera Mevalonate Pathway:

(13) Lepidoptera synthesize Juvenile Hormones through the mevalonate pathway as a signaling molecule to regulate body plan and development. While the Lepidoptera mevalonate pathway can catalyze the canonical reactions as mentioned above, it has a relaxed substrate specificity, allowing it to condense propionyl-CoA and acetyl-CoA at the first step of the pathway. Eventually this makes the six carbon building block HIPP, which can then be incorporated into the FPP analogues which become Juvenile Hormones. However, these same FPP analogues, if exposed to a suitable terpene cyclase can cyclize and become novel terpenes. FIG. 2.

(14) By heterologous expressing the mevalonate pathway enzymes (up to and including FPP synthases) from Lepidoptera species (or functionally equivalent enzymes of other species, particularly insects), coupled with a terpene cyclase, we have been able to make sixteen carbon terpenes. Because of the diversity of JHs available from nature, we can produce compounds containing seventeen and eighteen carbons as well. FIG. 4 shows FPP variants which occur in nature. Our system can also produce the precursor for making eleven and twelve carbon compounds, and by engineering a prenyltransferase(s), C21-24 compounds, C30-35 compounds and so on and so forth can also be created. The invention can be used to make already known terpenes with an ethyl substitution of a methyl group, as well as entirely new backbone configurations as the extra carbons allow novel ways for them to cyclize. These novel compounds have value in the areas where terpenes have traditionally found uses (pharmaceuticals, flavors, fragrances, commodity chemicals and fuels), and because of the modularity of the system, this invention greatly expands the reachable chemical space.

EXAMPLES

(15) In these examples we demonstrate expression constructs of Lepidoptera mevalonate pathway and cyclase for expression in E. coli.

(16) We selected the mevalonate pathway enzymes from Bombyx mori (silkworm) as predicted by Kinjoh T. et al, except for isopentenyl pyrophosphate isomerase (IPPI) and farnesyl pyrophosphate synthase (FPPS) which we sourced from Choristoneura fumiferana (eastern spruce budworm) since they have been studied previously. Soluble expression of HMGR required an N-terminal truncation to remove a membrane associated domaina similar truncation was required for S. cerevisiae HMGR soluble expression. We could not get the putative thiolase predicted by Kinjoh T. et al, to express in E. coli, so we used another well-characterized thiolase, PhaA from Acinetobacter sp. (strain RA3849).

(17) We constructed a pathway using two plasmids. The upper portion of the pathway, up to HMGR was expressed from a LacUV5 promoter, while the lower half up to IPPI was expressed from the Trc promoter on a plasmid containing the medium copy number p15a origin of replication. We named this construct pJH10 (FIG. 6). The FPP synthases and the well-studied epi-isozizaene synthase from Streptomyces coelicolor A3(2) were placed on a second plasmids under the control of pTrc promoter with the high copy number colE1 origin. We named this construct pJH15.

(18) As the expression strain we used the E. coli Bap1 cell line, a BL21DE3* derivative, which contains an inducible propionate CoA ligase. We transformed the cells and grew them in terrific broth supplemented with 0.4% glucose and 1 g/L sodium propionate with a nonane overlay to extract any produced terpenes. We showed that with the addition of the plasmids and propionate, we could see new 218 m/z peaks on GCMS corresponding to a C16 terpenes (FIG. 3). These peaks were contingent on propionate presence in the media. New profiles could be produced by using different cyclases and different mutants of epi-isozizaene synthase.

(19) Since the production of these new peaks was only detectable looking for the 218 m/z ion, we enriched our samples with a rotory evaporator and then used a scanning method to detect the fragmentation. Using this method, we were able to determine that the fragmentation pattern was also consistent with C16 terpenes. The 203 m/z fragment corresponds to a C16 terpene with a methyl group removed. Similarly, the 189 m/z peak corresponds with an ethyl group removed and the 175 m/z peak to a propyl group removed. (FIG. 3).

(20) Because the cell has high flux through acetyl-CoA, and the pathway can accept either acetoacetyl-CoA or 3-ketovaleryl-CoA, we have had difficulties directing flux to make C6 derived terpenes over C5 terpenes. To counter this, we leveraged fatty acid beta oxidation to make 3-ketovaleryl-CoA from exogenously added sodium valerate. We used the Keio collection JW2218 (which lacks the thiolase AtoB) and replaced PhaA from the pJH10 construct with a valeryl-CoA synthase from S. sativa and named the construct pJH53. In addition, we added FadB from E. coli and Aco from Micrococcus luteus behind a pLacUV5 promoter on the pJH15 construct and named the new construct pJH55 (FIG. 7). Via this method, we are able to greatly reduce the amount of C15 terpenes produced, while maintaining the production of C16 terpenes. Alternatively, we could use an enzyme which condenses propionyl-CoA and malonyl-CoA through a decarboxylative Claisen condensation to make 3-ketovaleryl-CoA.

(21) Our initial proof-of-principle demonstrated that the pathway will accept propionyl-CoA, but it can accept larger substrates as well including but not limited to acrylyl-CoA, butyryl-CoA, crotonyl-CoA, isobutyryl-CoA, cyclopropanecarboxyl-CoA, fluoroacetyl-CoA, chloroacetyl-CoA, bromoacetyl-CoA, propioyl-CoA. These alternatives further increase the reachable chemical space, especially if we can add functional groups, which greatly enable semi-synthesis efforts. (FIG. 5).

(22) As an example, we have made homomevalonate (FIG. 2, FIG. 8) as an intermediate on the way to C16 terpenes, but it itself can be used as a polymer or a co-polymer. Another alternative embodiment is homoisopentanol which can be used as a fuel or commodity chemical depending on pathways promiscuity, and homoisoprene and derivatives may find uses as polymers. (FIG. 8).