CATALYTIC POLYMERIZATION AND DEPOLYMERIZATION OF 1,6-ANHYDROSUGARS
20240279264 ยท 2024-08-22
Assignee
Inventors
Cpc classification
C08L5/00
CHEMISTRY; METALLURGY
C07H1/00
CHEMISTRY; METALLURGY
C08J11/10
CHEMISTRY; METALLURGY
C08B37/00
CHEMISTRY; METALLURGY
International classification
C07H1/00
CHEMISTRY; METALLURGY
Abstract
Methods for controlled synthesis and chemical recycling of stereoregular polysaccharides via polymerization of biomass-derived anhydrosugars are provided. The anhydrosugars react with an initiator in the presence of a synergy of a catalyst and the initiator providing the polysaccharides with high chain end fidelity and an excellent molecular weight distribution in a controlled manner, which allows for the preparation of polymeric systems with well-defined architectures. The polysaccharides obtained by such methods can be substantially converted to the start monomers in the present of a catalyst and can be used for biomedicine.
Claims
1. A method of controlled synthesizing and chemical recycling of a stereoregular ##STR00043## polysaccharide comprising reacting a biomass-derived anhydrosugar having the formula: wherein R is a group selected from C1-C6 alkyl group, with an initiator in the presence of a synergy of a catalyst and the initiator.
2. The method of claim 1, wherein the stereoregular polysaccharide is substantially converted to the anhydrosugar of Formula I by heating the stereoregular polysaccharide in the presence of the catalyst.
3. The method of claim 1, wherein the initiator is glucosyl halide having a structure represented by the formula: ##STR00044## wherein the halide is a group selected from fluoride, chloride, bromide or iodide.
4. The method of claim 1, wherein the catalyst is a Lewis acid.
5. The method of claim 4, wherein the Lewis acid is Cu(OTf).sub.2, BF.sub.3 or Boron trifluoride complex.
6. The method of claim 5, wherein the Boron trifluoride complex is boron trifluoride diethyl etherate.
7. The method of claim 1, wherein R is a group selected from C1-C7 alkyl group.
8. The method of claim 1, wherein R is a group selected from C1-C4 alkyl group.
9. The method of claim 1, wherein R is a group selected from C1-C3 alkyl group.
10. The method of claim 1, wherein R is a group selected from C1-C2 alkyl group.
11. The method of claim 1, wherein R is a methyl group.
12. The method of claim 1, wherein the initiator provides the polysaccharide with high chain end fidelity and an excellent molecular weight distribution in a controlled manner.
13. The method of claim 12, wherein the polysaccharide has 1-95% chain end fidelity.
14. The method of claim 12, wherein the polysaccharide has 50-95% chain end fidelity.
15. The method of claim 12, wherein the polysaccharide has about 90-95% chain end fidelity.
16. The method of claim 12, wherein a molecular weight of the polysaccharide ranges from 2.6 to 32.2 kDa.
17. A stereoregular polysaccharide prepared by the method of claim 1.
Description
BRIEF DESCRIPTION OF THE DRAWINGS
[0016] Many aspects of the present disclosure can be better understood with reference to the drawings. The components in the drawings are not necessarily to scale, emphasis instead being placed upon clearly illustrating the principles of the present disclosure. Moreover, in the drawings, like reference numerals designate corresponding parts throughout the several views. A better understanding of the features and advantages of the invention will be obtained by reference to the following detailed description that sets forth illustrative embodiments, in which the principles of the invention are utilized, and the accompanying drawings (also Figure and FIG. herein), of which:
[0017]
[0018]
[0019]
[0020]
[0021]
[0022] Additional advantages of the invention will be set forth in part in the description which follows, and in part will be obvious from the description, or can be learned by practice of the invention. The advantages of the invention will be realized and attained by means of the elements and combinations particularly pointed out in the appended claims. It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the invention, as claimed.
DETAILED DESCRIPTION OF THE INVENTION
[0023] Petroleum-based plastics are currently produced in a massive scale worldwide and contribute to a significant portion of the plastic waste accumulation in the environment. While much effort has been made to develop sustainable and degradable polymers (e.g., polyesters and polylactides) as potential replacements for traditional vinyl plastics, they are still single-use plastics that cannot be chemically recycled. The present disclosure provides a promising solution to this challenge. Starting from 1,6-anhydrosugar, an abundant natural product, a novel class of polysaccharides can be generated with precisely controlled molecular weight and stereochemistry. This unprecedented capability is of immediate and broad interest to the polymer industry, which has seen a significant surge of interest in addressing the global crisis of plastic pollution.
[0024] Disclosed herein is a novel approach that can achieve polysaccharides with high chain end fidelity and an excellent molecular weight distribution via polymerization of biomass-derived anhydrosugars in a controlled manner. The resulting polysaccharides possess excellent chemical stabilities against strong acid, base, and under high temperature. As a significant conceptual advance, the novel approach described herein has, surprisingly, enabled a fully closed recycling loop for the biomass-derived anhydrosugars using the same catalysts both in the polymerization to obtain the polysaccharides and unzipping thereof.
[0025] In one aspect, the disclosed methods provides that the anhydrosugars react with an initiator in the presence of a synergy of a catalyst and the initiator achieving the polysaccharides with high chain end fidelity and an excellent molecular weight distribution in a controlled manner. In a further aspect, the polysaccharides obtained by such methods can be recycled and depolymerized into the starting monomers in the presence of the catalyst.
[0026] Many modifications and other embodiments disclosed herein will come to mind to one skilled in the art to which the disclosed compositions and methods pertain having the benefit of the teachings presented in the foregoing descriptions and the associated drawings. Therefore, it is to be understood that the disclosures are not to be limited to the specific embodiments disclosed and that modifications and other embodiments are intended to be included within the scope of the appended claims. The skilled artisan will recognize many variants and adaptations of the aspects described herein. These variants and adaptations are intended to be included in the teachings of this disclosure and to be encompassed by the claims herein.
[0027] Although specific terms are employed herein, they are used in a generic and descriptive sense only and not for purposes of limitation.
[0028] As will be apparent to those of skill in the art upon reading this disclosure, each of the individual embodiments described and illustrated herein has discrete components and features which may be readily separated from or combined with the features of any of the other several embodiments without departing from the scope or spirit of the present disclosure.
[0029] Any recited method can be carried out in the order of events recited or in any other order that is logically possible. That is, unless otherwise expressly stated, it is in no way intended that any method or aspect set forth herein be construed as requiring that its steps be performed in a specific order. Accordingly, where a method claim does not specifically state in the claims or descriptions that the steps are to be limited to a specific order, it is no way intended that an order be inferred, in any respect. This holds for any possible non-express basis for interpretation, including matters of logic with respect to arrangement of steps or operational flow, plain meaning derived from grammatical organization or punctuation, or the number or type of aspects described in the specification.
[0030] All publications mentioned herein are incorporated herein by reference to disclose and describe the methods and/or materials in connection with which the publications are cited. The publications discussed herein are provided solely for their disclosure prior to the filing date of the present application. Nothing herein is to be construed as an admission that the present invention is not entitled to antedate such publication by virtue of prior invention. Further, the dates of publication provided herein can be different from the actual publication dates, which can require independent confirmation.
[0031] While aspects of the present disclosure can be described and claimed in a particular statutory class, such as the system statutory class, this is for convenience only and one of skill in the art will understand that each aspect of the present disclosure can be described and claimed in any statutory class.
[0032] It is also to be understood that the terminology used herein is for the purpose of describing particular aspects only and is not intended to be limiting. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the disclosed compositions and methods belong. It will be further understood that terms, such as those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the specification and relevant art and should not be interpreted in an idealized or overly formal sense unless expressly defined herein.
[0033] Prior to describing the various aspects of the present disclosure, the following definitions are provided and should be used unless otherwise indicated. Additional terms may be defined elsewhere in the present disclosure.
Definitions
[0034] As used herein, comprising is to be interpreted as specifying the presence of the stated features, integers, steps, or components as referred to, but does not preclude the presence or addition of one or more features, integers, steps, or components, or groups thereof. Moreover, each of the terms by, comprising, comprises, comprised of, including, includes, included, involving, involves, involved, and such as are used in their open, non-limiting sense and may be used interchangeably. Further, the term comprising is intended to include examples and aspects encompassed by the terms consisting essentially of and consisting of. Similarly, the term consisting essentially of is intended to include examples encompassed by the term consisting of.
[0035] As used in the specification and the appended claims, the singular forms a, an and the include plural referents unless the context clearly dictates otherwise. Thus, for example, reference to a monomer, a catalyst, or a polymer, includes, but is not limited to, mixtures or combinations of two or more such monomers, catalysts, or polymers, and the like.
[0036] It should be noted that ratios, concentrations, amounts, and other numerical data can be expressed herein in a range format. It will be further understood that the endpoints of each of the ranges are significant both in relation to the other endpoint, and independently of the other endpoint. It is also understood that there are a number of values disclosed herein, and that each value is also herein disclosed as about that particular value in addition to the value itself. For example, if the value 10 is disclosed, then about 10 is also disclosed. Ranges can be expressed herein as from about one particular value, and/or to about another particular value. Similarly, when values are expressed as approximations, by use of the antecedent about, it will be understood that the particular value forms a further aspect. For example, if the value about 10 is disclosed, then 10 is also disclosed.
[0037] When a range is expressed, a further aspect includes from the one particular value and/or to the other particular value. For example, where the stated range includes one or both of the limits, ranges excluding either or both of those included limits are also included in the disclosure, e.g., the phrase x to y includes the range from x to y as well as the range greater than x and less than y. The range can also be expressed as an upper limit, e.g., about x, y, z, or less and should be interpreted to include the specific ranges of about x, about y, and about z as well as the ranges of less than x, less than y, and less than z. Likewise, the phrase about x, y, z, or greater should be interpreted to include the specific ranges of about x, about y, and about z as well as the ranges of greater than x, greater than y, and greater than z. In addition, the phrase about x to y, where x and y are numerical values, includes about x to about y.
[0038] It is to be understood that such a range format is used for convenience and brevity, and thus, should be interpreted in a flexible manner to include not only the numerical values explicitly recited as the limits of the range, but also to include all the individual numerical values or sub-ranges encompassed within that range as if each numerical value and sub-range is explicitly recited. To illustrate, a numerical range of about 0.1% to 5% should be interpreted to include not only the explicitly recited values of about 0.1% to about 5%, but also include individual values (e.g., about 1%, about 2%, about 3%, and about 4%) and the sub-ranges (e.g., about 0.5% to about 1.1%; about 5% to about 2.4%; about 0.5% to about 3.2%, and about 0.5% to about 4.4%, and other possible sub-ranges) within the indicated range.
[0039] As used herein, the terms about, approximate, at or about, and substantially mean that the amount or value in question can be the exact value or a value that provides equivalent results or effects as recited in the claims or taught herein. That is, it is understood that amounts, sizes, formulations, parameters, and other quantities and characteristics are not and need not be exact but may be approximate and/or larger or smaller, as desired, reflecting tolerances, conversion factors, rounding off, measurement error and the like, and other factors known to those of skill in the art such that equivalent results or effects are obtained. In some circumstances, the value that provides equivalent results or effects cannot be reasonably determined. In such cases, it is generally understood, as used herein, that about and at or about mean the nominal value indicated ?10% variation unless otherwise indicated or inferred. In general, an amount, size, formulation, parameter or other quantity or characteristic is about, approximate, or at or about whether or not expressly stated to be such. It is understood that where about, approximate, or at or about is used before a quantitative value, the parameter also includes the specific quantitative value itself, unless specifically stated otherwise.
[0040] As used herein, the terms optional or optionally means that the subsequently described event or circumstance can or cannot occur, and that the description includes instances where said event or circumstance occurs and instances where it does not.
[0041] Unless otherwise specified, temperatures referred to herein are based on atmospheric pressure (i.e., one atmosphere).
[0042] The composition, sequence, length, and type of glycosidic linkages of polysaccharides profoundly affect their biological and physical properties. However, investigation of the structure-function relationship of polysaccharides is hampered by accessing well-defined polysaccharides in sufficient quantities. Here, the present disclosure provides a chemical approach to precision polysaccharides with native glycosidic linkages via living cationic ring-opening polymerization of 1,6-anhydrosugars. In certain embodiments, well-defined polysaccharides with tunable molecular weight, low dispersity, and excellent regio- and stereoselectivity were synthesized using a boron trifluoride etherate catalyst and glycosyl fluoride initiators. Computational studies revealed that the reaction propagated through the monomer a-addition to the oxocarbenium and was controlled by the reversible deactivation of the propagating oxocarbenium to form the glycosyl fluoride dormant species. The method disclosed herein afforded a facile and scalable pathway to multiple biologically relevant precision polysaccharides, including D-glucan, D-mannan, and an unusual L-glucan. It is demonstrated that catalytic depolymerization of precision polysaccharides efficiently regenerated monomers, suggesting their utility as a class of chemically recyclable materials with tailored thermal and mechanical properties.
[0043] In certain embodiments, the synergistic combination of a glycosyl donor initiator and a mild Lewis acid catalyst were leveraged to achieve living CROP of 1,6-anhydrosugars (
[0044] The present disclosure provides a chemical approach to precision native polysaccharides through living polymerization of 1,6-anhydrosugars. The generality of this method enabled the facile synthesis of a variety of polysaccharides and oligo-glycans with excellent regio- and stereoselectivity, precise molecular weight control, and high fidelity of the chain end groups. In addition, the obtained materials displayed excellent chemical recyclability and tunable thermal and mechanical properties. Overall, the method disclosed herein created a new paradigm for the chemical synthesis of polysaccharides with strong implications in a range of applications spanning from materials science to bioengineering.
[0045] Now having described the aspects of the present disclosure, in general, the following Examples describe some additional aspects of the present disclosure. While aspects of the present disclosure are described in connection with the following examples and the corresponding text and figures, there is no intent to limit aspects of the present disclosure to this description. On the contrary, the intent is to cover all alternatives, modifications, and equivalents included within the spirit and scope of the present disclosure.
EXAMPLES
[0046] The following examples are put forth so as to provide those of ordinary skill in the art with a complete disclosure and description of how the compounds, compositions, articles, devices and/or methods claimed herein are made and evaluated and are intended to be purely exemplary of the disclosure and are not intended to limit the scope of what the inventors regard as their disclosure. Efforts have been made to ensure accuracy with respect to numbers (e.g., amounts, temperature, etc.), but some errors and deviations should be accounted for. Unless indicated otherwise, parts are parts by weight, temperature is in ? C. or is at ambient temperature, and pressure is at or near atmospheric.
Example 1
Materials and Methods
[0047] All manipulations of air- and moisture-sensitive materials were carried out under nitrogen in a glovebox or by using standard Schlenk line techniques. Solvents for polymerization, such as Dichloromethane (DCM), chloroform (CHCl.sub.3) and acetonitrile (CH.sub.3CN), were dried over calcium hydride for 24 hrs, distillated to an oven-dried Schlenk flask, and stored in a nitrogen gas-filled glovebox. Solvents for air sensitive reactions, such as tetrahydrofuran (THF), dimethylformamide (DMF), were purchased from Fisher Scientific and used after purification by a dry solvent system (Pure Process Technology). Otherwise, solvents (hexane, acetone, toluene, EtOAc, MeOH, DMSO) were used as received. BF.sub.3.Math.Et.sub.2O was dried over calcium hydride for 24 hrs, vacuum distillated to an oven-dried Schlenk flask, and stored under nitrogen in a glovebox freezer at ?30? C. All other chemicals were purchased from Alfa Aesar, Sigma-Aldrich, Acros, Fisher Scientific, or TCI chemical companies and used as received without further purification.
[0048] .sup.1H, .sup.13C, .sup.19F NMRSpectra were recorded on either a Varian Gemini-600 (600 MHz) or Varian Inova-500 (500 MHZ) NMR spectrometer Chemical shifts (?) for .sup.1H and .sup.13C NMR spectra were referenced to protons of the residual solvent (for .sup.1H) or deuterated solvent itself (for .sup.13C).
[0049] Powder X-Ray Diffraction (PXRD)Analyses were performed on a Rigaku MiniFlex diffractometer. The voltage was set to 40 kV, and the current was 40 mA. The detector collected data over the range 2?=5-50?.
[0050] Specific RotationAnalyses were performed on a Rudolph Analytical Research Autopol IV Polarimeter. The wavelength of the light used is 589 nanometers (the sodium D line), and the path length is 0.5 dm. The data was collected at 20? C.
[0051] High-resolution mass spectrometry (HRMS)Analyses were performed on JEOL AccuTOF DART Micromass LCT ESI-MS.
[0052] Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS)Analyses were performed on a Bruker Auto Flex Max instrument (positive mode). ?-Cyano-4-hydroxycinnamic acid was used as matrix with NaI added as the cation source.
[0053] Size-exclusion chromatography (SEC)Analyses were carried out using a Tosoh's high performance SEC system HLC-8320GPC with a refractive index detector. The Tosoh SEC system was equipped with two TSKgel GMH.sub.HR-N columns (5 ?m, 7.8 mm ID), which were eluted with CHCl.sub.3 at 40? C. at a rate of 0.5 mL/min and calibrated using polystyrene standards (ReadyCal Kit, Sigma-Aldrich #81434). Deprotected polymer molecular weights were determined by SEC using DMF (0.01 M LiBr) as the eluent at a flow rate of 0.5 mL/min through three TSKgel AlphaM columns (13 ?m, 7.8 mm ID) at 50? C. with a refractive index detector.
[0054] Thermogravimetric Analysis (TGA)Thermal gravimetric analysis was obtained using a Netzsch Instruments STA 449 F1 Jupiter. Analysis was performed on ?10 mg of a given sample at a heating rate of 10? C./min from 25 to 600? C. under nitrogen gas.
[0055] Differential Scanning calorimetry (DSC)Data was recorded on a Netzsch instruments DSC 214 Polyma using ?10 mg samples. All T.sub.m and T.sub.g values were obtained from a second scan after the thermal history was removed from the first scan. The second heating rate was 10? C./min and cooling rate was 10? C./min.
[0056] Uniaxial Tensile Elongation TestsAnalyses were performed on an Instron 5567 universal testing system equipped with a 1 kN load cell and manual grips, at a crosshead speed of 5 mm/min. A pre-strain of 0.1 N was also applied. The samples were prepared in Teflon molds with cavity dimensions of (63.5?9.5?3.2 mm.sup.3). Grip separation was used as the gauge length for the purposes of calculating strain. Extension to failure was performed using three parallel samples and the data were analyzed using the Bluehill Universal Software. The Young's modulus was calculated using the slope of the stress-strain curve from 0 to 1% strain.
[0057] Monomer Synthesis
##STR00003##
[0058] General procedure: To a suspension of NaH in DMF was added 1,6-anhydro-?-D-glucopyranose at 0? C., and the mixture was stirred for additional 30 mins. Subsequently, alkyl halide was added slowly at 0? C. After being allowed to warm up to 25? C., the reaction was left stirring for 12 hrs. The reaction was quenched with MeOH, and the solvent was removed under reduced pressure. DCM was added, successively washed with water and brine, and then dried over Na.sub.2SO.sub.4. After filtration, all the volatiles were removed under vacuum and the crude was purified by flash chromatography to afford the desired product.
##STR00004##
[0059] Prepared according to the general procedure using 1,6-anhydro-?-D-glucose (10 g, 61.7 mmol, 1.0 equiv), NaH (8.6 g, 215.9 mmol, 3.5 equiv), methyl iodide (13.4 mL, 215.9 mmol, 3.5 equiv) in DMF (150 mL) at 25? C. The crude was purified by flash chromatography (hexane/ethyl acetate=2/1) to afford the desired product Me-D-Glc as a white solid (11.8 g, 94% yield). .sup.1H NMR spectra is consistent with literature data.sup.41. .sup.1H NMR (500 MHZ, CDCl.sub.3): ? 5.47 (s, 1H), 4.62 (dd, J=6.0, 1.6 Hz, 1H), 3.91 (dd, J=7.2, 0.8 Hz, 1H), 3.72 (dd, J=7.2, 6.0 Hz, 1H), 3.47 (s, 3H), 3.46 (s, 3H), 3.44 (s, 3H), 3.33-3.31 (m, 1H), 3.14-3.12 (m, 1H), 3.10-3.08 (m, .sup.1H).
##STR00005##
[0060] Prepared according to the general procedure using 1,6-anhydro-?-D-glucose (10 g, 61.7 mmol, 1.0 equiv), NaH (8.6 g, 215.9 mmol, 3.5 equiv), ethyl iodide (17.3 mL, 215.9 mmol, 3.5 equiv) in DMF (150 mL) at 25? C. The crude was purified by flash chromatography (hexane/ethyl acetate=6/1) to afford the desired product Et-D-Glc as a colorless liquid (14.0 g, 92% yield). .sup.1H NMR (600 MHZ, CDCl.sub.3): ? 5.38 (s, 1H), 4.54 (dd, J=6.0, 1.4 Hz, 1H), 3.88 (dd, J=7.0, 1.4 Hz, 1H), 3.69-3.66 (m, 1H), 3.64-3.55 (m, 6H), 3.38-3.36 (m, 1H), 3.19-3.17 (m, 1H), 3.16-3.13 (m, 1H), 1.24-1.17 (m, 9H). .sup.13C NMR (125 MHZ, CDCl.sub.3): ? 100.57, 78.46, 77.87, 77.28, 74.22, 65.54, 65.35, 65.27, 64.69, 15.24, 15.17, 15.15. HRMS (DART-MS) m/z calcd. for C.sub.12H.sub.23O.sub.5 [M+H].sup.+ 247.15400. found: 247.15431.
##STR00006##
[0061] Prepared according to the general procedure using 1,6-anhydro-?-D-glucose (2.0 g, 12.3 mmol, 1.0 equiv), NaH (2.2 g, 55.5 mmol, 4.5 equiv), 1-iodopropane (5.4 mL, 55.5 mmol, 4.5 equiv) in DMF (70 mL) at 50? C. The crude was purified by flash chromatography (hexane/ethyl acetate=9/1) to afford the product .sup.nPr-D-Glc as a colorless liquid (2.1 g, 62% yield). .sup.1H NMR (600 MHZ, CDCl.sub.3): ? 5.40 (s, 1H), 4.56 (dd, J=6.0, 1.4 Hz, 1H), 3.89 (dd, J=7.0, 1.4 Hz, 1H), 3.69 (dd, J=7.0, 6.0 Hz, 1H), 3.55-3.45 (m, 6H), 3.38-3.36 (m, 1H), 3.19-3.17 (m, 1H), 3.16-3.13 (m, 1H), 1.66-1.56 (m, 6H), 0.95-0.91 (m, 9H). .sup.13C NMR (125 MHZ, CDCl.sub.3): ? 100.86, 79.04, 78.32, 77.49, 74.54, 72.19, 71.87, 71.35, 65.65, 23.14, 23.02, 23.00, 10.61, 10.56, 10.51. HRMS (DART-MS) m/z calcd. for C.sub.15H.sub.29O.sub.5 [M+H].sup.+ 289.20095. found: 289.20084.
##STR00007##
[0062] Prepared according to the general procedure using 1,6-anhydro-?-D-glucose (2.0 g, 12.3 mmol, 1.0 equiv), NaH (2.2 g, 55.5 mmol, 4.5 equiv), 1-iodobutane (6.3 mL, 55.5 mmol, 4.5 equiv) in DMF (70 mL) at 50? C. The crude was purified by flash chromatography (hexane/ethyl acetate=12/1) to afford the product .sup.nBu-D-Glc as a colorless liquid (2.4 g, 59% yield). .sup.1H NMR (600 MHZ, CDCl.sub.3): ? 5.40 (s, 1H), 4.54 (dd, J=5.8, 1.4 Hz, 1H), 3.88 (dd, J=7.0, 1.2 Hz, 1H), 3.69 (dd, J=7.0, 5.8 Hz, 1H), 3.60-3.49 (m, 6H), 3.37-3.34 (m, 1H), 3.18-3.16 (m, 1H), 3.15-3.13 (m, 1H), 1.61-1.53 (m, 6H), 1.42-1.36 (m, 6H), 0.94-0.90 (m, 9H). .sup.13C NMR (150 MHZ, CDCl.sub.3): ? 100.85, 79.03, 78.29, 77.46, 74.53, 70.26, 69.90, 69.40, 65.65, 32.01, 31.87, 31.85, 19.33, 19.27, 19.21, 13.84, 13.82. HRMS (DART-MS) m/z calcd. for C.sub.18H.sub.35O.sub.5 [M+H].sup.+ 331.24790. found: 331.24691.
##STR00008##
[0063] Prepared according to the general procedure using 1,6-anhydro-?-D-glucose (2.0 g, 12.3 mmol, 1.0 equiv), NaH (2.2 g, 55.5 mmol, 4.5 equiv), 1-bromopentane (6.9 mL, 55.5 mmol, 4.5 equiv) in DMF (70 mL) at 50? C. The crude was purified by flash chromatography (hexane/ethyl acetate=15/1) to afford the product .sup.nPen-D-Glc as a colorless liquid (3.3 g, 71% yield). .sup.1H NMR (500 MHZ, CDCl.sub.3): ? 5.40 (s, 1H), 4.54 (dd, J=5.8, 1.4 Hz, 1H), 3.87 (dd, J=7.0, 1.4 Hz, 1H), 3.69 (dd, J=7.0, 5.8 Hz, 1H), 3.59-3.48 (m, 6H), 3.37-3.34 (m, 1H), 3.18-3.16 (m, 1H), 3.15-3.13 (m, 1H), 1.63-1.56 (m, 6H), 1.36-1.31 (m, 12H), 0.92-0.88 (m, 9H). .sup.13C NMR (150 MHz, CDCl.sub.3): ? 100.86, 79.07, 78.36, 77.51, 74.55, 70.62, 70.25, 69.75, 65.67, 29.62, 29.51, 29.49, 28.34, 28.29, 28.22, 22.48, 22.46, 14.00, 13.98. HRMS (DART-MS) m/z calcd. for C.sub.21H.sub.41O.sub.5 [M+H].sup.+ 373.29485. found: 373.29423.
##STR00009##
[0064] Prepared according to the general procedure using 1,6-anhydro-?-D-glucose (1.6 g, 10 mmol, 1.0 equiv), NaH (1.4 g, 35.0 mmol, 3.5 equiv), allyl bromide (3.0 mL, 35.0 mmol, 3.5 equiv) in DMF (20 mL) at 25? C. The crude was purified by flash chromatography (hexane/ethyl acetate=6/1) to afford the product All-D-Glc as a colorless liquid (2.7 g, 96% yield). .sup.1H NMR spectra is consistent with literature data.sup.42. .sup.1H NMR (500 MHZ, CDCl.sub.3): ? 5.98-5.86 (m, 3H), 5.43 (s, 1H), 5.33-5.27 (m, 3H), 5.22-5.19 (m, 3H), 4.58 (dd, J=5.8, 1.4 Hz, 1H), 4.14-4.09 (m, 6H), 3.92 (dd, J=7.2, 1.2 Hz, 1H), 3.70 (dd, J=7.2, 5.8 Hz, 1H), 3.52-3.50 (m, 1H), 3.32-3.30 (m, 1H), 3.28-3.26 (m, .sup.1H).
##STR00010##
[0065] Prepared according to the general procedure using 1,6-anhydro-?-D-mannose (1.0 g, 6.2 mmol, 1.0 equiv), NaH (0.87 g, 21.6 mmol, 3.5 equiv), allyl bromide (1.9 mL, 21.6 mmol, 3.5 equiv) in DMF (15 mL) at 25? C. The crude was purified by flash chromatography (hexane/ethyl acetate=4/1) to afford the product All-D-Man as a colorless liquid (1.5 g, 86% yield). .sup.1H NMR (500 MHZ, CDCl.sub.3): ? 5.97-5.87 (m, 3H), 5.44 (s, 1H), 5.32-5.17 (m, 6H), 4.52 (d, J=5.8, Hz, 1H), 4.21 (d, J=7.0, Hz, 1H), 4.16-4.07 (m, 6H), 3.79-3.76 (m, 1H), 3.74 (t, J=6.5, Hz, 1H), 3.53-3.50 (m, 2H). .sup.13C NMR (125 MHz, CDCl.sub.3): ? 134.83, 134.52, 134.38, 117.80, 117.72, 117.38, 100.12, 76.57, 74.45, 74.33, 73.95, 72.56, 70.50, 70.40, 64.86. HRMS (DART-MS) m/z calcd. for C.sub.15H.sub.23O.sub.5 [M+H].sup.+ 283.15400. found: 283.15507.
##STR00011##
[0066] Prepared according to the general procedure using 1,6-anhydro-?-D-galactose (0.5 g, 3.1 mmol, 1.0 equiv), NaH (0.45 g, 10.8 mmol, 3.5 equiv), ethyl iodide (0.9 mL, 10.8 mmol, 3.5 equiv) in DMF (15 mL) at 25? C. The crude was purified by flash chromatography (hexane/ethyl acetate=6/1) to afford the desired product Et-D-Gal as a colorless liquid (0.64 g, 84% yield). .sup.1H NMR (500 MHZ, CDCl.sub.3): ? 5.37 (s, 1H), 4.42-4.38 (m, 2H), 3.72-3.69 (m, 1H), 3.68-3.66 (m, 1H), 3.65-3.55 (m, 7H), 3.41-3.38 (m, 1H), 1.24-1.18 (m, 9H). .sup.13C NMR (125 MHz, CDCl.sub.3): ? 100.18, 77.80, 75.41, 73.50, 72.96, 66.97, 65.93, 64.56, 64.23, 15.50, 15.47, 15.45. HRMS (DART-MS) m/z calcd. for C.sub.12H.sub.23O.sub.5 [M+H].sup.+ 247.15400. found: 247.15430.
##STR00012##
[0067] Prepared according to the general procedure using 1,6-anhydro-?-L-glucose.sup.43 (1.0 g, 6.2 mmol, 1.0 equiv), NaH (1.1 g, 27.7 mmol, 4.5 equiv), 1-bromobutane (3.0 mL, 27.7 mmol, 4.5 equiv) in DMF (30 mL) at 50? C. The crude was purified by flash chromatography (hexane/ethyl acetate=12/1) to afford the product .sup.nBu-L-Glc as a colorless liquid (1.2 g, 59% yield). .sup.1H NMR (500 MHZ, CDCl.sub.3): ? 5.40 (s, 1H), 4.55 (dd, J=5.8, 1.4 Hz, 1H), 3.87 (dd, J=7.0, 1.2 Hz, 1H), 3.69 (dd, J=7.0, 5.8 Hz, 1H), 3.59-3.49 (m, 6H), 3.37-3.34 (m, 1H), 3.18-3.16 (m, 1H), 3.15-3.13 (m, 1H), 1.62-1.52 (m, 6H), 1.43-1.35 (m, 6H), 0.94-0.90 (m, 9H).
##STR00013##
[0068] Prepared according to the general procedure using 1,6-anhydro-?-L-glucose.sup.43 (0.82 g, 5.0 mmol, 1.0 equiv), NaH (0.7 g, 17.5 mmol, 3.5 equiv), allyl bromide (1.5 mL, 17.5 mmol, 3.5 equiv) in DMF (15 mL) at 25? C. The crude was purified by flash chromatography (hexane/ethyl acetate=6/1) to afford the product All-L-Glc as a colorless liquid (1.2 g, 85% yield). .sup.1H NMR (500 MHZ, CDCl.sub.3): ? 5.98-5.86 (m, 3H), 5.43 (s, 1H), 5.33-5.27 (m, 3H), 5.22-5.19 (m, 3H), 4.58 (dd, J=5.8, 1.4 Hz, 1H), 4.14-4.09 (m, 6H), 3.92 (dd, J=7.2, 1.2 Hz, 1H), 3.70 (dd, J=7.2, 5.8 Hz, 1H), 3.52-3.50 (m, 1H), 3.32-3.30 (m, 1H), 3.28-3.26 (m, .sup.1H).
Initiator Synthesis
[0069] ##STR00014##
I-1, I-S2, I-S3, I-2 were prepared according to literature procedures.sup.44-47.
##STR00015##
[0070] Literature procedures were modified for the preparation of I-S1.sup.48. A solution of I-2 (450 mg, 0.8 mmol, 1.0 equiv) in acetone (3 mL) was added dropwise to the suspension of sodium ethyl trithiocarbonate (155 mg, 1.0 mmol, 1.2 equiv) in acetone (3 mL) over 10 mins at 25? C. The resulting mixture was stirred for 2 hrs, filtered through Celite, washed with ethyl acetate. The solvent was removed in vacuo and the crude was purified by flash chromatography (hexane/ethyl acetate=10/1) to afford the desired product I-S1 as a yellow syrup (400 mg, 76% yield). .sup.1H NMR (500 MHZ, CDCl.sub.3) ? 7.38-7.25 (m, 18H), 7.19-7.15 (m, 2H), 5.70 (d, J=10.1 Hz, 1H), 4.90 (q, J=11.0 Hz, 2H), 4.82 (d, J=10.9 Hz, .sup.1H), 4.77 (s, 2H), 4.60 (dd, J=25.1, 11.4 Hz, 2H), 4.50 (d, J=12.1 Hz, 1H), 3.85-3.72 (m, 4H), 3.67 (dd, J=10.2, 8.4 Hz, 1H), 3.65-3.60 (m, 1H), 3.38 (q, J=7.4 Hz, 2H), 1.36 (t, J=7.5 Hz, 3H). .sup.13C NMR (151 MHZ, CDCl.sub.3): ? 220.70, 138.34, 138.05, 137.50, 128.43, 128.38, 128.31, 128.20, 127.93, 127.89, 127.80, 127.72, 127.69, 127.59, 86.92, 86.43, 79.77, 79.48, 77.58, 75.76, 75.54, 74.96, 73.43, 68.41, 31.39, 12.87. HRMS (DART-MS) m/z calcd. for C.sub.37H.sub.44NO.sub.5S.sub.3 [M+NH.sub.4].sup.+ 678.23761. found: 678.23755.
##STR00016##
[0071] DAST (0.7 mL, 5.5 mmol, 1.1 equiv) was added dropwise over 10 min to a stirring solution of 2,3,4,6-tetra-O-benzyl-D-glucopyranose (2.7 g, 5.0 mmol, 1.0 equiv) in dry CH.sub.2Cl.sub.2 (25 mL) at 0? C., and the reaction was left stirring for 1 hr. Saturated aqueous NaHCO.sub.3 (5 mL) was added, and the mixture was stirred vigorously for 15 mins. Then the organic phase was isolated and washed with brine, and the organic extract was dried over Na.sub.2SO.sub.4. After filtration, all the volatiles were removed under vacuum and purified by flash chromatography (hexane/ethyl acetate=10/1), yielding the desired product I-3 (2.5 g, 92% yield, ?/?=1/4) as a colorless syrup. .sup.1H NMR for I-3 was essentially the same as reported previously.sup.49.
##STR00017##
[0072] DAST (0.3 mL, 2.2 mmol, 1.1 equiv) was added dropwise over 10 min to a stirring solution of 2,3,4,6-tetra-O-benzyl-L-glucopyranose.sup.50 (1.1 g, 2.0 mmol, 1.0 equiv) in dry CH.sub.2Cl.sub.2 (10 mL) at 0? C., and the reaction was left stirring for 1 hr. Saturated aqueous NaHCO.sub.3 (5 mL) was added, and the mixture was stirred vigorously for 15 mins. Then the organic phase was isolated and washed with brine, and the organic extract was dried over Na.sub.2SO.sub.4. After filtration, all the volatiles were removed under vacuum and purified by flash chromatography (hexane/ethyl acetate=10/1), yielding the desired product ent-I-3 (1.0 g, 90% yield, ?/?=1/2.2) as a colorless syrup. .sup.1H NMR for ent-I-3 was essentially the same as reported previously.sup.49.
##STR00018##
[0073] DAST (0.5 mL, 3.4 mmol, 1.1 equiv) was added dropwise over 10 min to a stirring solution of 2,3,4,6-tetra-O-benzyl-D-mannopyranose.sup.50 (1.7 g, 3.1 mmol, 1.0 equiv) in dry CH.sub.2Cl.sub.2 (20 mL) at 0? C., and the reaction was left stirring for 1 hr. Saturated aqueous NaHCO.sub.3 (5 mL) was added, and the mixture was stirred vigorously for 15 mins. Then the organic phase was isolated and washed with brine, and the organic extract was dried over Na.sub.2SO.sub.4. After filtration, all the volatiles were removed under vacuum and purified by flash chromatography (hexane/ethyl acetate=12/1), yielding the desired product I-4 (1.5 g, 88% yield) as a colorless syrup. .sup.1H NMR spectra is consistent with literature data.sup.51.
Crosslinker Synthesis
[0074] ##STR00019##
[0075] To a solution of CL-Me-S1.sup.52 (2.0 g, 9.8 mmol, 1.0 equiv) and silver triflate (10.1 g, 39.2 mmol, 4.0 equiv) in CH.sub.2Cl.sub.2 (70 mL) under a nitrogen atmosphere at 0? C., was added diisopropylethylamine (5.1 mL, 29.4 mmol, 3.0 equiv) slowly followed by methyl iodide (2.0 mL, 31.4 mmol, 3.2 equiv) dropwise. The reaction was stirred at 25? C. for 5 hrs, the brown reaction mixture was then filtered through a pad of silica gel, washing with ethyl acetate. The solvent was removed in vacuo and the crude was purified by flash chromatography (hexane/ethyl acetate=1/1) to afford the desired product CL-Me-S2 as a white solid (2.0 g, 88% yield). .sup.1H NMR spectra is consistent with literature data.sup.53. .sup.1H NMR (500 MHZ, CDCl.sub.3): ? 5.48 (s, 1H), 5.00-4.97 (m, 1H), 4.65 (dd, J=6.0, 1.4 Hz, 1H), 3.97 (dd, J=7.4, 1.2 Hz, 1H), 3.72 (dd, J=7.4, 6.0 Hz, 1H), 3.51 (s, 3H), 3.50 (s, 3H), 3.11-3.09 (m, 1H), 3.06-3.04 (m, 1H), 2.11 (s, 3H).
[0076] To a solution of CL-Me-S2 (2.0 g, 8.6 mmol, 1.0 equiv) in MeOH (20 mL) at 25? C., was added MeONa (46.5 mg, 0.86 mmol, 0.1 equiv). The reaction was stirred at 25? C. for 2 hrs and neutralized by Amberlite resin IR-120 (H.sup.+). After filtration, the solvent was removed in vacuo to furnish the desired product CL-Me-S3 as a colorless syrup, the syrup was used for next step without further purification.
[0077] Prepared according to a modified literature procedure.sup.54. To a solution of 1,4-butanediol dimethanesulfonate (740 mg, 3.0 mmol, 1.0 equiv) and CL-Me-S3 (860 mg, 4.5 mmol, 1.5 equiv) in toluene (2.5 mL) and dimethyl sulfoxide (10 mL) was added KOH (675 mg, 12.0 mmol, 4.0 equiv). The reaction was stirred at 80? C. for 12 hrs, after cooled down to room temperature, the mixture was diluted with ethyl acetate. The organic layer was washed with brine and dried over Na.sub.2SO.sub.4. After filtration, the solvent was removed in vacuo and the crude was purified by flash chromatography (hexane/ethyl acetate=1/2) to afford the desired product CL-Me as a colorless syrup (800 mg, 81% yield). .sup.1H NMR (500 MHZ, CDCl.sub.3): ? 5.46 (s, 2H), 4.62 (dd, J=6.0, 1.6 Hz, 2H), 3.93 (dd, J=7.2, 1.2 Hz, 2H), 3.71 (dd, J=7.2, 6.0 Hz, 2H), 3.61-3.51 (m, 4H), 3.46 (s, 6H), 3.45 (s, 6H), 3.41-3.39 (m, 2H), 3.13-3.11 (m, 2H), 3.09-3.07 (m, 2H), 1.67-1.63 (m, 4H). .sup.13C NMR (125 MHz, CDCl.sub.3): ? 99.87, 79.52, 78.61, 76.74, 73.10, 69.99, 65.14, 57.80, 57.12, 26.71. HRMS (DART-MS) m/z calcd. for C.sub.20H.sub.35O.sub.10 [M+H].sup.+ 435.22247. found: 435.22181.
Polymer Synthesis
[0078] General procedure: In a glovebox under a nitrogen atmosphere, an oven-dried vial was charged with [M], [I] and DCM (or CDCl.sub.3), BF.sub.3.Math.Et.sub.2O was added. After 6 hrs, the reaction vial was removed from the glovebox and quenched with two drops of methanol. The polymer solution was precipitated into cold hexane or MeOH/H.sub.2O (v/v, 1/1), centrifuged, discarded the solvent, and redissolve in CHCl.sub.3. This procedure was repeated three times to ensure any catalyst residue or unreacted monomer was removed. The polymer was dried under high vacuum overnight to a constant weight.
##STR00020##
[0079] Prepared according to the general procedure using Me-D-Glc (510.6 mg, 2.5 mmol), I-3 (0.2 M, 250 ?L, 50 ?mol), CDCl.sub.3 (488 ?L) and BF.sub.3.Math.Et.sub.2O (0.2 M, 12.5 ?L, 2.5 ?mol), the polymer solution was precipitated into cold hexane to give a fine white powder P(Me-D-Glc) (510.7 mg, 95% yield). .sup.1H NMR (600 MHZ, CDCl.sub.3): ? 4.94 (d, J=3.8 Hz, .sup.1H), 3.83-3.76 (m, 1H), 3.70-3.62 (m, 1H), 3.58 (s, 3H), 3.51 (s, 3H), 3.48-3.45 (m, 1H), 3.43 (s, 3H), 3.20 (t, J=9.4 Hz, 1H), 3.11 (dd, J=9.6, 3.6 Hz, .sup.1H). .sup.13C NMR (125 MHZ, CDCl.sub.3): ? 96.34, 83.26, 81.72, 79.44, 70.18, 65.91, 60.62, 60.41, 58.17. .sup.19F NMR (470 MHz, CDCl.sub.3): ? ?149.14 (dd, J=53.1, 25.7 Hz). SEC (CHCl.sub.3): M.sub.n=5.8 kDa, ?=1.23. [?].sub.D.sup.20=201.3 (c 1.08, CHCl.sub.3).
##STR00021##
[0080] Prepared according to the general procedure using Et-D-Glc (49.3 mg, 48 ?L, 0.2 mmol), I-3 (0.4 M, 5 ?L, 2 ?mol), CDCl.sub.3 (70 ?L) and BF.sub.3.Math.Et.sub.2O (0.02 M, 10 ?L, 0.2 ?mol), the polymer solution was precipitated into cold hexane to give a fine white powder P(Et-D-Glc) (35.6 mg, 70% yield). .sup.1H NMR (600 MHZ, CDCl.sub.3): ? 4.93 (d, J=3.6 Hz, 1H), 3.87-3.80 (m, 2H), 3.75-3.62 (m, 6H), 3.56-3.50 (m, 2H), 3.34 (t, J=9.4 Hz, 1H), 3.14 (dd, J=9.6, 3.4 Hz, 1H), 1.20-1.13 (m, 9H). .sup.13C NMR (125 MHZ, CDCl.sub.3): ? 96.99, 81.27, 80.33, 77.52, 70.73, 68.40, 68.13, 65.76, 65.43, 15.83, 15.81, 15.65. .sup.19F NMR (470 MHZ, CDCl.sub.3): ? ?148.86 (dd, J=53.4, 25.7 Hz). SEC (CHCl.sub.3): M.sub.n=12.0 kDa, ?=1.23. [?].sub.D.sup.20=171.7 (c 1.21, CHCl.sub.3).
##STR00022##
[0081] Prepared according to the general procedure using .sup.nPr-D-Glc (86.5 mg, 84 ?L, 0.3 mmol), I-3 (0.2 M, 15 ?L, 3 ?mol), DCM (42 ?L) and BF.sub.3.Math.Et.sub.2O (0.01 M, 9 ?L, 0.09 ?mol), the polymer solution was precipitated into MeOH/H.sub.2O (v/v, 1/1) to give a fine white powder P(.sup.nPr-D-Glc) (44.7 mg, 52% yield). .sup.1H NMR (600 MHZ, CDCl.sub.3): ? 4.92 (d, J=3.4 Hz, 1H), 3.80-3.72 (m, 3H), 3.66-3.50 (m, 6H), 3.42-3.35 (m, 2H), 3.14 (dd, J=9.6, 3.4 Hz, 1H), 1.61-1.51 (m, 6H), 0.94-0.86 (m, 9H). .sup.13C NMR (125 MHZ, CDCl.sub.3): ? 96.99, 81.39, 80.71, 77.40, 74.88, 74.49, 72.08, 70.80, 65.44, 23.64, 23.50, 23.31, 10.62, 10.58, 10.55. .sup.19F NMR (470 MHZ, CDCl.sub.3): ? ?151.44 (dd, J=53.5, 25.3 Hz). SEC (CHCl.sub.3): M.sub.n=16.7 kDa, ?=1.38. [?].sub.D.sup.20=146.1 (c 0.62, CHCl.sub.3).
##STR00023##
[0082] Prepared according to the general procedure using .sup.nBu-D-Glc (99.1 mg, 96 ?L, 0.3 mmol), I-3 (0.2 M, 15 ?L, 3 ?mol), tetrabutylammonium difluorotriphenylsilicate (0.001 M, 18 ?L, 0.018 ?mol), DCM (3 ?L) and BF.sub.3.Math.Et.sub.2O (0.01 M, 18 ?L, 0.18 ?mol), the polymer solution was precipitated into MeOH/H.sub.2O (v/v, 1/1) to give a colorless syrup P(.sup.nBu-D-Glc) (58.6 mg, 59% yield). .sup.1H NMR (600 MHZ, CDCl.sub.3): ? 4.91 (d, J=3.4 Hz, 1H), 3.82-3.72 (m, 3H), 3.66-3.50 (m, 6H), 3.45-3.35 (m, 2H), 3.13 (dd, J=9.6, 3.4 Hz, 1H), 1.61-1.51 (m, 6H), 1.41-1.31 (m, 6H), 0.94-0.86 (m, 9H). .sup.13C NMR (125 MHZ, CDCl.sub.3): ? 97.14, 81.46, 80.79, 77.25, 73.03, 72.62, 70.86, 70.22, 65.47, 32.71, 32.53, 32.27, 19.37, 19.29, 19.28, 14.01, 13.98, 13.93. .sup.19F NMR (470 MHZ, CDCl.sub.3): ? ?148.88 (dd, J=53.3, 26.0 Hz). SEC (CHCl.sub.3): M.sub.n=18.1 kDa, ?=1.13. [?].sub.D.sup.20=132.8 (c 0.95, CHCl.sub.3).
##STR00024##
[0083] Prepared according to the general procedure using .sup.nPen-D-Glc (111.8 mg, 108 ?L, 0.3 mmol), I-3 (0.2 M, 15 ?L, 3 ?mol), tetrabutylammonium difluorotriphenylsilicate (0.001 M, 18 ?L, 0.018 ?mol) and BF.sub.3.Math.Et.sub.2O (0.01 M, 18 ?L, 0.18 ?mol), the polymer solution was precipitated into MeOH/H.sub.2O (v/v, 1/1) to give a colorless syrup P(.sup.nPen-D-Glc) (69.4 mg, 62% yield). .sup.1H NMR (600 MHZ, CDCl.sub.3): ? 4.91 (d, J=3.4 Hz, 1H), 3.82-3.72 (m, 3H), 3.66-3.50 (m, 6H), 3.45-3.35 (m, 2H), 3.13 (dd, J=9.6, 3.4 Hz, 1H), 1.63-1.45 (m, 6H), 1.37-1.25 (m, 12H), 0.92-0.86 (m, 9H). .sup.13C NMR (125 MHZ, CDCl.sub.3): ? 97.25, 81.43, 80.79, 77.12, 73.36, 72.87, 70.92, 70.48, 65.43, 30.28, 30.12, 29.86, 28.41, 28.29, 22.65, 22.58, 22.55, 14.04, 14.01. .sup.19F NMR (470 MHZ, CDCl.sub.3): ? ?148.81 (dd, J=53.5, 25.9 Hz). SEC (CHCl.sub.3): M.sub.n=18.6 kDa, ?=1.16. [?].sub.D.sup.20=118.4 (c 1.13, CHCl.sub.3).
##STR00025##
[0084] Prepared according to the general procedure using All-D-Glc (141.2 mg, 140 ?L, 0.5 mmol), I-3 (0.4 M, 125 ?L, 50 ?mol) and BF.sub.3.Math.Et.sub.2O (0.2 M, 4 ?L, 0.8 ?mol), the polymer solution was precipitated into MeOH/H.sub.2O (v/v, 1/1) to give a colorless syrup P(All-D-Glc) (126.0 mg, 75% yield). .sup.1H NMR (600 MHZ, CDCl.sub.3): ? 6.00-5.80 (m, 3H), 5.31-5.21 (m, 3H), 5.18-5.06 (m, 3H), 4.98-4.90 (m, 1H), 4.36-4.32 (m, 2H), 4.26-4.20 (m, 1H), 4.18-4.08 (m, 3H), 3.85-3.76 (m, 1H), 3.73-3.62 (m, 3H), 3.55-3.45 (m, 1H), 3.35-3.25 (m, .sup.1H). .sup.13C NMR (125 MHZ, CDCl.sub.3): ? 135.53, 135.15, 116.39, 116.08, 97.15, 81.13, 79.50, 77.13, 73.99, 73.68, 71.23, 70.70, 65.50. .sup.19F NMR (470 MHZ, CDCl.sub.3): ? ?139.33 (dd, J=53.2, 12.9 Hz), ?149.06 (dd, J=53.2, 25.5 Hz). SEC (CHCl.sub.3): M.sub.n=2.6 kDa, ?=1.18. [?].sub.D.sup.20=121.0 (c 1.67, CHCl.sub.3).
##STR00026##
[0085] Prepared according to the general procedure using .sup.nBu-L-Glc (66.1 mg, 68 ?L, 0.2 mmol), ent-I-3 (0.2 M, 10 ?L, 2 ?mol), DCM (10 ?L) and BF.sub.3.Math.Et.sub.2O (0.02 M, 10 ?L, 0.2 ?mol), the polymer solution was precipitated into MeOH/H.sub.2O (v/v, 1/1) to give a colorless syrup P(.sup.nBu-L-Glc) (46.3 mg, 70% yield). .sup.1H NMR (600 MHZ, CDCl.sub.3): ? 4.91 (d, J=3.4 Hz, 1H), 3.82-3.72 (m, 3H), 3.66-3.48 (m, 6H), 3.45-3.35 (m, 2H), 3.13 (dd, J=9.6, 3.4 Hz, 1H), 1.61-1.49 (m, 6H), 1.42-1.30 (m, 6H), 0.94-0.86 (m, 9H). .sup.13C NMR (125 MHZ, CDCl.sub.3): ? 97.13, 81.45, 80.78, 77.25, 73.03, 72.61, 70.85, 70.23, 65.46, 32.70, 32.53, 32.26, 19.36, 19.29, 19.27, 14.01, 13.98, 13.92. .sup.19F NMR (470 MHZ, CDCl.sub.3): ? ?151.42 (dd, J=53.2, 25.8 Hz). SEC (CHCl.sub.3): M.sub.n=18.5 kDa, ?=1.21. [?].sub.D.sup.20=?133.9 (c 0.67, CHCl.sub.3).
##STR00027##
[0086] Prepared according to the general procedure using All-L-Glc (141.2 mg, 140 ?L, 0.5 mmol), ent-I-3 (0.4 M, 125 ?L, 50 ?mol) and BF.sub.3.Math.Et.sub.2O (0.2 M, 5 ?L, 1.0 ?mol), the polymer solution was precipitated into MeOH/H.sub.2O (v/v, 1/1) to give a colorless syrup P(All-L-Glc) (131.1 mg, 78% yield). .sup.1H NMR (600 MHZ, CDCl.sub.3): ? 6.00-5.80 (m, 3H), 5.31-5.21 (m, 3H), 5.18-5.06 (m, 3H), 4.98-4.90 (m, 1H), 4.36-4.32 (m, 2H), 4.26-4.21 (m, .sup.1H), 4.18-4.06 (m, 3H), 3.85-3.75 (m, 1H), 3.73-3.62 (m, 3H), 3.55-3.45 (m, 1H), 3.35-3.25 (m, .sup.1H). .sup.13C NMR (125 MHZ, CDCl.sub.3): ? 135.51, 135.13, 116.40, 116.09, 97.14, 81.11, 79.48, 77.10, 73.99, 73.67, 71.23, 70.68, 65.47. .sup.19F NMR (470 MHZ, CDCl.sub.3): ? ?139.32 (dd, J=53.2, 12.9 Hz), ?149.05 (dd, J=53.2, 25.5 Hz). SEC (CHCl.sub.3): M.sub.n=2.6 kDa, ?=1.17. [?].sub.D.sup.20=?123.4 (c 1.50, CHCl.sub.3).
##STR00028##
[0087] Prepared according to the general procedure using All-D-Man (56.5 mg, 56 ?L, 0.2 mmol), 1-4 (0.4 M, 33.5 ?L, 13.4 ?mol) and BF.sub.3.Math.Et.sub.2O (0.2 M, 2.5 ?L, 0.5 ?mol), the polymer solution was precipitated into MeOH/H.sub.2O (v/v, 1/1) to give a colorless syrup P(All-D-Man) (51.9 mg, 81% yield). .sup.1H NMR (600 MHZ, CDCl.sub.3): ? 5.98-5.79 (m, 3H), 5.35-5.07 (m, 6H), 5.00-4.87 (m, 1H), 4.41-4.34 (m, 1H), 4.20-4.00 (m, 6H), 3.90-3.86 (m, .sup.1H), 3.82-3.78 (m, 1H), 3.75-3.70 (m, 2H), 3.61-3.59 (m, .sup.1H). .sup.13C NMR (125 MHz, CDCl.sub.3): ? 135.25, 134.94, 134.52, 116.78, 116.63, 115.92, 98.62, 79.41, 75.23, 74.08, 73.72, 71.90, 71.61, 70.87, 66.12. .sup.19F NMR (470 MHZ, CDCl.sub.3): ? ?138.04 (d, J=50.7 Hz). SEC (CHCl.sub.3): M.sub.n=3.3 kDa, ?=1.24. [?].sub.D.sup.20=131.1 (c 2.33, CHCl.sub.3).
##STR00029##
[0088] Prepared according to the general procedure using Et-D-Gal (24.6 mg, 24 ?L, 0.1 mmol), I-3 (0.2 M, 10 ?L, 2 ?mol), TBAT (0.001 M, 6 ?L, 0.006 ?mol), DCM (7 ?L) and BF.sub.3.Math.Et.sub.2O (0.01 M, 4 ?L, 0.04 ?mol), the polymer solution was precipitated into cold hexane to give a fine white powder P(Et-D-Gal) (16.7 mg, 68% yield). .sup.1H NMR (600 MHZ, CDCl.sub.3): ? 4.87-4.75 (m, 1H), 4.03-3.92 (m, 2H), 3.90-3.83 (m, 1H), 3.78-3.50 (m, 9H), 1.20-1.13 (m, 9H). .sup.13C NMR (125 MHZ, CDCl.sub.3): ? 98.53, 79.83, 75.53, 74.07, 71.76, 68.10, 66.23, 65.97, 65.42, 15.80, 15.62, 15.49. .sup.19F NMR (470 MHz, CDCl.sub.3): ? ?150.17 (dd, J=53.9, 25.5 Hz). SEC (CHCl.sub.3): M.sub.n=6.4 kDa, ?=1.14. [?].sub.D.sup.20=143.2 (c 0.73, CHCl.sub.3).
Glycan Synthesis
[0089] ##STR00030##
[0090] Prepared according to a modified literature procedure.sup.55. To an oven-dried 10 mL Schlenk flask equipped with a stir bar was added P(All-D-Glc) (100 mg), 1,3-dimethylbarbituric acid (DMBA, 300 mg, 6 equiv), Pd(PPh.sub.3).sub.4 (74 mg, 0.2 equiv), HOAc (120 ?L), CHCl.sub.3 (1.2 mL) and MeOH (2.4 mL). Then the reaction mixture was stirred vigorously for 12 hrs at 60? C. After cooled down to room temperature, the solvent was removed, H.sub.2O (0.4 mL) was added. The mixture was precipitated into acetone, centrifuged, discarded the solvent, and redissolve in H.sub.2O. This procedure was repeated three times, after that, the mixture was further purified by reverse phase column (20-80% MeOH/H.sub.2O) to yield a white foam P(OH.sup.Bn-D-Glc) after lyophilization. .sup.1H NMR (600 MHZ, D.sub.2O): ? 4.99 (s, 1H), 4.05-3.96 (m, 1H), 3.95-3.89 (m, 1H), 3.80-3.70 (m, 2H), 3.63-3.57 (m, 1H), 3.55-3.50 (m, .sup.1H). .sup.13C NMR (151 MHZ, D.sub.2O): ? 97.62, 73.32, 71.32, 70.10, 69.45, 65.46. .sup.19F NMR (470 MHZ, D.sub.2O): ? ?150.4-?151.2 (br). SEC (DMF): M.sub.n=1.7 kDa, ?=1.20. [?].sub.D.sup.20=137.6 (c 0.093, H.sub.2O).
[0091] The P(OH.sup.Bn-D-Glc) (20 mg) was dissolved in a mixture of H.sub.2O/MeOH (1 mL/1 mL) containing 20% Pd(OH).sub.2/C (6 mg, 30 wt %). The resulting mixture was stirred under H.sub.2 atmosphere (1 atm) for 12 hrs at 25? C., and was then filtrated through a pad of Celite, washed with H.sub.2O/MeOH (v/v, 1/1) three times. The filtrates were concentrated, and the crude was purified by reverse phase column (20-50% MeOH/H.sub.2O) to yield a white foam P(OH-D-Glc) after lyophilization. .sup.1H NMR (600 MHZ, D.sub.2O): ? 5.00-4.98 (m, 1H), 4.05-3.98 (m, 1H), 3.95-3.89 (m, 1H), 3.80-3.70 (m, 2H), 3.65-3.57 (m, 1H), 3.55-3.49 (m, .sup.1H). .sup.13C NMR (125 MHZ, D.sub.2O): ? 97.67, 73.36, 71.36, 70.15, 69.50, 65.52. .sup.19F NMR (470 MHz, D.sub.2O): ? ?150.82 (dd, J=53.4, 26.5 Hz). [?].sub.D.sup.20=150.8 (c 0.122, H.sub.2O).
##STR00031##
[0092] To an oven-dried 10 mL Schlenk flask equipped with a stir bar was added P(All-L-Glc) (100 mg), 1,3-dimethylbarbituric acid (DMBA, 300 mg, 6 equiv), Pd(PPh.sub.3).sub.4 (74 mg, 0.2 equiv), HOAc (120 ?L), CHCl.sub.3 (1.2 mL) and MeOH (2.4 mL). Then the reaction mixture was stirred vigorously for 12 hrs at 60? C. After cooled down to room temperature, the solvent was removed, H.sub.2O (0.4 mL) was added. The mixture was precipitated into acetone, centrifuged, discarded the solvent, and redissolve in H.sub.2O. This procedure was repeated three times, after that, the mixture was further purified by reverse phase column (20-80% MeOH/H.sub.2O) to yield a white foam P(OH.sup.Bn-L-Glc) after lyophilization. .sup.1H NMR (600 MHZ, D.sub.2O): ? 5.00 (s, 1H), 4.05-3.96 (m, 1H), 3.95-3.88 (m, 1H), 3.81-3.71 (m, 2H), 3.63-3.57 (m, 1H), 3.56-3.50 (m, .sup.1H). .sup.13C NMR (151 MHz, D.sub.2O): ? 97.66, 73.35, 71.35, 70.13, 69.49, 65.50. .sup.19F NMR (470 MHZ, D.sub.2O): ? ?150.5-?151.0 (br). SEC (DMF): M.sub.n=1.7 kDa, ?=1.22. [?].sub.D.sup.20=?137.6 (c 0.170, H.sub.2O).
[0093] The P(OH.sup.Bn-L-Glc) (20 mg) was dissolved in a mixture of H.sub.2O/MeOH (1 mL/1 mL) containing 20% Pd(OH).sub.2/C (6 mg, 30 wt %). The resulting mixture was stirred under H.sub.2 atmosphere (1 atm) for 12 hrs at 25? C., and was then filtrated through a pad of Celite, washed with H.sub.2O/MeOH (v/v, 1/1) three times. The filtrates were concentrated, and the crude was purified by reverse phase column (20-50% MeOH/H.sub.2O) to yield a white foam P(OH-L-Glc) after lyophilization. .sup.1H NMR (600 MHZ, D.sub.2O): ? 5.01-4.96 (m, 1H), 4.04-3.97 (m, 1H), 3.96-3.88 (m, 1H), 3.80-3.71 (m, 2H), 3.62-3.57 (m, 1H), 3.56-3.51 (m, .sup.1H). .sup.13C NMR (125 MHZ, D.sub.2O): ? 97.67, 73.36, 71.37, 70.15, 69.50, 65.51. .sup.19F NMR (470 MHz, D.sub.2O): 0-150.83 (dd, J=53.2, 26.4 Hz). [?].sub.D.sup.20=?149.4 (c 0.087, H.sub.2O).
##STR00032##
[0094] To an oven-dried 10 mL Schlenk flask equipped with a stir bar was added P(All-D-Man) (100 mg), 1,3-dimethylbarbituric acid (DMBA, 300 mg, 6 equiv), Pd(PPh.sub.3).sub.4 (74 mg, 0.2 equiv), CHCl.sub.3 (1.2 mL) and MeOH (2.4 mL). Then the reaction mixture was stirred vigorously for 12 hrs at 60? C. After cooled down to room temperature, the solvent was removed, H.sub.2O (0.4 mL) was added. The mixture was precipitated into acetone, centrifuged, discarded the solvent, and redissolve in H.sub.2O. This procedure was repeated three times, after that, the mixture was further purified by reverse phase column (20-80% MeOH/H.sub.2O) to yield a white foam P(OH.sup.Bn-D-Man) after lyophilization. .sup.1H NMR (600 MHZ, D.sub.2O): ? 4.92 (s, 1H), 4.05-3.70 (m, 6H). .sup.13C NMR (125 MHZ, D.sub.2O): ? 99.31, 70.80, 70.66, 69.95, 66.58, 65.49. .sup.19F NMR (470 MHZ, D.sub.2O): ? ?150.5-?151.0 (br). SEC (DMF): M.sub.n=2.0 kDa, ?=1.26. [?].sub.D.sup.20=45.3 (c 0.053, H.sub.2O).
[0095] The P(OH.sup.Bn-D-Man) (20 mg) was dissolved in a mixture of H.sub.2O/MeOH (1 mL/1 mL) containing 20% Pd(OH).sub.2/C (6 mg, 30 wt %). The resulting mixture was stirred under H.sub.2 atmosphere (1 atm) for 12 hrs at 25? C., and was then filtrated through a pad of Celite, washed with H.sub.2O/MeOH (v/v, 1/1) three times. The filtrates were concentrated, and the crude was purified by reverse phase column (20-50% MeOH/H.sub.2O) to yield a white foam P(OH-D-Man) after lyophilization. .sup.1H NMR (600 MHZ, D.sub.2O): ? 4.94-4.88 (m, 1H), 4.01 (s, .sup.1H), 3.98-3.91 (m, 1H), 3.90-3.83 (m, 2H), 3.82-3.78 (m, 1H), 3.76-3.70 (m, .sup.1H). .sup.13C NMR (125 MHZ, D.sub.2O): ? 99.32, 70.81, 70.67, 69.96, 66.58, 65.49. .sup.19F NMR (470 MHZ, D.sub.2O): ? ?139.00 (d, J=47.3 Hz). [?].sub.D.sup.20=60.0 (c 0.080, H.sub.2O).
Chain Extensions
[0096] ##STR00033##
[0097] P(.sup.nBu-D-Glc) was prepared according to the general procedure with [M].sub.0:[I].sub.0=33. The macroinitiator P(.sup.nBu-D-Glc) (7 mg) and Me-D-Glc (10.2 mg) was dissolve in CDCl.sub.3 (40 ?L), BF.sub.3.Math.Et.sub.2O (0.2 M, 0.6 ?L, 0.12 ?mol) was added. The polymer solution was precipitated into MeOH/H.sub.2O (v/v, 1/1) to give a diblock copolysaccharide P(.sup.nBu-D-Glc)-b-P(Me-D-Glc) as a white powder. P(.sup.nPen-D-Glc)-b-P(Me-D-Glc) was prepared through a similar procedure, except P(.sup.nPen-D-Glc) was used as a macroinitiator. The successful chain extension was confirmed by a complete shift to higher molecular weight, a monomodal distribution with low D and little to no tailing in SEC traces, as well as a single peak in the diffusion-ordered spectroscopy (DOSY) NMR analysis.
Chain End Modifications
[0098] ##STR00034##
[0099] P(Me-D-Glc) was prepared according to the general procedure with [M].sub.0:[I].sub.0=15. P(Me-D-Glc) (34 mg) was dissolved in CDCl.sub.3 (0.2 mL), alkenyl alcohol (0.5 M, 27 ?L, 13.5 ?mol) and BF.sub.3.Math.Et.sub.2O (0.2 M, 25 ?L, 5 ?mol) was sequentially added. After 1 hr, the reaction was quenched with two drops of methanol. The polymer solution was precipitated into cold hexane to give P(Me.sup.O1-D-Glc) as a white powder. P(Me.sup.O2-D-Glc) was prepared through a similar procedure, except alkynyl was used as a nucleophile. The .sup.1H-NMR and MALDI-TOF MS analyses indicated that the transformations of ?-F chain end were quantitative.
Enzymatic Degradation Studies
[0100] Adapted from a literature report.sup.56: P(OH.sup.Bn-D-Glc) or P(OH.sup.Bn-L-Glc) solutions (1.0 mg, 10 mg/mL) in ammonium acetate (100 ?L, 5 mM, pH 5.5) were degraded by dextranase (from Penicillium funiculosum, 0.5 U/mL) at 37? C. for 72 hrs. The mixtures were lyophilized to provide white foams. The molecular weights were determined by SEC using DMF (0.01 M LiBr) as the eluent. SEC analyses revealed complete degradation of D-glucan, while L-glucan remained intact.
Chemical Resistance Studies
[0101] P(Me-D-Glc) (50 mg, M.sub.n=7.8 kDa, ?=1.23) was dissolved in CHCl.sub.3 (2.0 mL), additive (5 equiv relative to repeating units) was added. After 24 hrs, the polymer solution was precipitated into cold methanol to remove additive. SEC analysis show little to no change on M.sub.n and dispersity for the polymer treated by various additives.
Thermodynamic Studies
[0102] In a glovebox under a nitrogen atmosphere, an oven-dried J. Young NMR tube was charged with Et-D-Glc (200 ?L, 0.8 mmol), I-3 (0.2 M, 40 ?L, 8 ?mol) and 282 ?L of CDCl.sub.3. BF.sub.3.Math.Et.sub.2O (0.2 M, 8 ?L, 1.6 ?mol) was added ([Et-D-Glc].sub.0=1.5 M), the tube was sealed and inverted three times to ensure adequate mixing of the reagents. The NMR tube was then taken out of the glovebox and brought into a 600 MHZ NMR probe preheated to the desired polymerization temperature (25, 33, 40, 50 and 60? C., respectively). The polymerization was monitored by .sup.1H NMR (no. scans=8, delay=5 s) at different time intervals until the conversion remained constant at each temperature. The equilibrium monomer concentration, [Et-D-Glc].sub.eq, was calculated to be 0.090, 0.120, 0.180, 0.225 and 0.330 M for 25, 33, 40, 50 and 60? C., respectively (Table 5), based on the equation [Et-D-Glc].sub.eq=[Et-D-Glc].sub.0?(1?conv.). The thermodynamic parameters were calculated to be ?H=?26.8 kJ.Math.mol.sup.?1 and ?S=?69.7 J.Math.mol.sup.?1.Math.K.sup.?1 by the linear fitting of the plot of Rln[Et-D-Glc].sub.eq vs. 1/T according to Van't Hoff equation: Rln[Et-D-Glc].sub.eq=?H/T??S. Therefore, the ceiling temperature was calculated to be T.sub.c=93? C. for [Et-D-Glc].sub.0=1.0 M, based on the equation T.sub.c.sup.o=?H.sup.o/?S.sup.o=?H/(?S?Rln[Et-D-Glc].sub.0), assuming ?H=?H.sup.o
Depolymerization Studies
Depolymerzation of P(Me-D-Glc) Under Thermolysis Condition
[0103] ##STR00035##
[0104] To an oven-dried 5 mL Schlenk flask equipped with a condenser tube and a receiving flask was added P(Me-D-Glc) (93.0 mg), the system was then connected to house vacuum (30 mmHg). The reaction was heated for 10 mins at 450? C. After cooled down to room temperature, the liquid in the condenser tube and receiving flask was collected, rinsed with CHCl.sub.3. All the volatiles were removed under vacuum and the crude was purified by flash chromatography to afford a light-yellow liquid (31.6 mg, 34% yield), .sup.1H NMR analysis showed that monomer Me-D-Glc was recovered in a pure state.
Depolymerization of P(Et-D-Glc) Under Chemolysis Condition
[0105] ##STR00036##
[0106] An oven-dried 50 mL Schlenk flask was charged with P(Et-D-Glc) (400 mg), CH.sub.2Cl.sub.2 (15 mL) and BF.sub.3.Math.Et.sub.2O (7.5 ?L, 60 ?mol, 5 mol %) was added. The reaction mixture was heated for 2 hrs at 80? C. After cooled down to room temperature, the resulting mixture was then filtered through a pad of silica gel, washing with ethyl acetate. The solvent was removed in vacuo and the crude was purified by flash chromatography (hexane/ethyl acetate=2/1) to afford the desired product Et-D-Glc as a light-yellow liquid (346 mg, 86% yield). .sup.1H NMR analysis showed that monomer Et-D-Glc was recovered in a pure state.
Depolymerization of Bulk Materials (PN1, PN2) Under Chemolysis Condition
[0107] An oven-dried 250 mL Schlenk flask was charged with bulk materials PN1 and PN2 (6.0 g), CH.sub.2Cl.sub.2 (120 mL) and BF.sub.3.Math.Et.sub.2O (150 ?L, 1.2 mmol, 5 mol %) was added. The reaction mixture was heated for 2 hrs at 80? C. After cooled down to room temperature, the resulting mixture was then filtered through a pad of silica gel, washing with ethyl acetate. The solvent was removed in vacuo and the crude was purified by flash chromatography (hexane/ethyl acetate=10/1 to 2/1 to 1/2) to afford the desired product Me-D-Glc (1.76 g), .sup.nPen-D-Glc (2.71 g) and CL-Me (0.60 g). .sup.1H NMR analysis showed that both monomers and crosslinker was recovered in a pure state, and the recycle yield was estimated to be 85%. In addition, the recovered anhydrosugars could be repolymerized to give PN1/PN2 with identical tensile properties to pristine material.
Polymer Film Preparation
[0108] ##STR00037##
[0109] General procedure: In a glovebox under a nitrogen atmosphere, an oven-dried vial was charged with Me-D-Glc, .sup.nPen-D-Glc, CL-Me and I-3. To this solution was added BF.sub.3.Math.Et.sub.2O, and the mixture was transferred into a dog-bone mold (dimensions: 63.5?9.5?3.2 mm.sup.3). The sample was allowed to cure at room temperature for 12 hrs. The specimen was carefully removed from the mold, the residual monomer and catalyst were removed by Soxhlet extraction with MeOH. The sample was dried under high vacuum overnight to a constant weight.
[0110] PN1/PN1 was prepared according to the general procedure using Me-D-Glc (114.4 mg, 560 ?mol), .sup.nPen-D-Glc (357.6 mg, 384 ?L, 960 ?mol), CL-Me (1.0 M, 80 ?L, 80 ?mol), I-3 (0.2 M, 80 ?L, 16 ?mol) and BF.sub.3.Math.Et.sub.2O (0.2 M, 70 ?L, 14 ?mol).
[0111] PN2/PN2 was prepared according to the general procedure using Me-D-Glc (196.1 mg, 960 ?mol), .sup.nPen-D-Glc (208.6 mg, 224 ?L, 560 ?mol), CL-Me (1.0 M, 80 ?L, 80 ?mol), I-3 (0.2 M, 80 ?L, 16 ?mol), DCM (110 ?L) and BF.sub.3.Math.Et.sub.2O (0.2 M, 50 ?L, 10 ?mol).
Conditions Optimization
[0112] General procedure: In a glovebox under a nitrogen atmosphere, an oven-dried vial was charged with Me-D-Glc (20.5 mg, 0.1 mmol), I-3 (0.2 M, 10 ?L, 2 ?mol), and solution of Lewis acid in DCM or CH.sub.3CN was added to give a final reaction concentration of 2.0 M. After 3 hrs, the reaction vial was removed from the glovebox and quenched with two drops of methanol. The polymer solution was precipitated into cold hexane, centrifuged, discarded the solvent, and redissolve in CHCl.sub.3. This procedure was repeated three times to ensure any catalyst residue or unreacted monomer was removed. The polymer was dried under high vacuum overnight to a constant weight. The experimental results were summarized in Table 1 below.
Example 2
Preparation and Characterization of Precision Polysaccharides
[0113] From the outset, O-methylated 1,6-anhydro-b-D-glucopyranose Me-D-Glc was utilized as a model monomer, which can be readily obtained after a one-step methylation of the biomass-derived and commercially available 1,6-anhydro-b-D-glucopyranose ($1.3/g) in high yield and decagram scale (94%, 11.8 g). Previous works by Kamigaito.sup.21 and Fors.sup.22 have shown that thiocarbamate chain transfer agents could regulate the propagation of oxocarbenium ions, which are structurally like the proposed propagating species in the polymerization of 1,6-anhydrosugars (
[0114] Table 1. Summary of conditions optimization results for Me-D-Glc. Monomer conversion was determined by .sup.1H NMR spectroscopy; M.sub.n,GPC and ? were determined by SEC analysis calibrated to polystyrene standard; w-chain end fidelity was determined by NMR analysis using w-fidelity=I.sup.5.55-5.66 ppm/(I.sup.7.10-7.12 ppm/2), where I.sup.5.55-5.66 ppm and I.sup.7.10-7.12 ppm correspond to integrals of signal at 5.55-5.66 ppm and 7.10-7.12 ppm attributed to hydrogen of the w-chain end and two hydrogens of the a-chain end, respectively.
TABLE-US-00001 TABLE 1
[0115] While I-1 in conjunction with Cu(OTf).sub.2 produced a polysaccharide with a number-average molecular weight (M.sub.n) of 6.9 kg/mol, the high dispersity (?=1.54) and the low fidelity of the w-chain end suggested poor control over the polymerization (
Example 2
Monomer Scope
[0116] With the initiator/catalyst pair identified, the monomer scope of this polymerization was explored (
[0117] Table 2. Summary of polymerization results for various monomers. The polymerizations were performed at 25? C. in dichloromethane for 6 hrs; monomer conversion was determined by .sup.1H NMR spectroscopy; M.sub.n,Theo was calculated using the following equation: M.sub.n,Theo=[M].sub.0/[I].sub.0?MW.sup.M?conv.+MW.sup.I, where MW.sup.M and MW.sup.I correspond to molar mass of monomer and initiator, respectively; M.sub.n,NMR was calculated using the following equation: M.sub.n,NMR=DP?MW.sup.M+MW.sup.I, and DP was determined by NMR analysis using the following equation: DP=I.sup.4.90-5.00 ppm/(I.sup.7.10-7.12 ppm/2), where I.sup.4.90-5.00 ppm and I.sup.7.10-7.12 ppm correspond to integrals of signal at 4.90-5.00 ppm and 7.10-7.12 ppm attributed to anomeric hydrogen of the polymer backbone and two hydrogens of the a-chain end, respectively; M.sub.n,GPC and ? were determined by SEC analysis calibrated to polystyrene standard.
TABLE-US-00002 TABLE 2
Example 3
Computational Investigations
[0118] Density functional theory (DFT) calculations were performed to gain more mechanistic insights into the reversible deactivation process in the living CROP and the origin of the excellent a-selectivity during the propagation step (
[0119] As for the propagation process, two sequential steps were then considered. A monomer first attacked the oxocarbenium to form an oxonium species 3, and then a subsequent ring opening reaction occurred to regenerate the propagating oxocarbenium. According to the DFT calculations, the energy barrier of the a-addition was lower than that of the b-addition (TS.sub.2_3a=4.9 kcal mol.sup.?1 versus TS.sub.2_3b=9.9 kcal mol.sup.?1). The difference in transition state energies is primarily attributed to the steric repulsion between the incoming anhydrosugar monomer and oxocarbenium species. This a-selectivity is consistent with the experimental results, where the single anomeric carbon signal was observed in .sup.13C NMR spectra of the precision polysaccharides. A ring opening process was identified after the formation of oxonium intermediate 3a, which is considerably exergonic by 6.7 kcal mol.sup.?1, indicative of the facile regeneration of the oxocarbenium. It is noteworthy that the direct addition to oxonium 3a by the anhydrosguar monomer proposed by Schuerch.sup.17 yielded no computationally viable transition states despite extensive searches. This result is consistent with a recent work by Reineke.sup.19.
Example 4
Chain Extension and Chain End Modifications
[0120] Synthesizing advanced polymeric architectures, such as block copolymers, represents one of the greatest advantages of living polymerization. Building upon this method, block copolysaccharides that are otherwise laborious to synthesize were accessed. First, polymerization of .sup.nBu-D-Glc was carried out in the presence of I-3 to give a macroinitiator P(.sup.nBu-D-Glc) (M.sub.n=3.2 kg/mol, ?=1.15) with an excellent fidelity of the w-chain end. Next, chain extension of P(.sup.nBu-D-Glc) by Me-D-Glc yielded a diblock copolysaccharide P(.sup.nBu-D-Glc)-b-P(Me-D-Glc). A shift to higher molecular weight, monomodal distribution with low ? (1.17) and little to no tailing in SEC traces (
[0121] Furthermore, as glycosyl fluoride has been widely used as a glycosyl donor in catalytic glycosylation reactions, the w-F chain end presents a versatile handle for further functionalization. As a proof of this principle, glycosylation of alkenyl and alkynyl alcohol by the w-F chain end of P(Me-D-Glc) was examined using BF.sub.3.Math.Et.sub.2O as an activator (
Example 5
Synthesis of Native Polysaccharides
[0122] Oligomeric a-1,6-glycans play significant roles in many biological processes.sup.25. Therefore, the current polymerization method was applied to access these biologically important glycans. Because of the facile removal of allyl groups, O-allyl 1,6-D-anhydroglucose All-D-Glc was chosen as the monomer. Polymerization of All-D-Glc at [M].sub.0:[I].sub.0 ratio of 10:1 yielded a precision polysaccharide P(All-D-Glc) with excellent molecular weight control (
[0123] Biosynthetic pathways of polysaccharides predominantly produce D-enantiomers which are susceptible to enzymatic degradation.sup.27. In contrast, a chemical polymerization process does not discriminate either D- or L-anhydrosugars, providing a good opportunity to generate physiologically stable all-L-polysaccharides. Indeed, in the presence of a L-glycosyl fluoride initiator ent-I-3, 1,6-anhydro-b-L-glucopyranose .sup.nBu-L-Glc and All-L-Glc could be polymerized to give the corresponding all-L-polysaccharide without any erosion in efficiency and livingness (
[0124] Furthermore, it was demonstrated the synthetic utility of the current living CROP strategy to synthesize a-1,6-D-mannan, a polysaccharide that exists in the cell wall of Mycobacterium tuberculosis and is implicated in the human immune response to pathogens.sup.28. To produce precision a-1,6-D-mannan, All-D-Man was first polymerized into a well-defined polysaccharide P(All-D-Man) (M.sub.n=3.3 kDa, ?=1.24). After removing the allyl groups, a well-defined a-1,6-D-mannan was readily generated (
Example 6
Depolymerization and Repolymerization
[0125] Beyond biologically active polysaccharides, renewable materials were also explored based on precision polysaccharides. One of the most desired properties of the next-generation sustainable polymers is their inherent chemical recyclability, which enables a closed-loop life cycle.sup.30-33. Given that 1,6-anhydroglucose was produced by the thermolysis of starch or cellulose, precision polysaccharide P(Me-D-Glc) was first subjected to thermolysis conditions (450? C., 30 mmHg). Indeed, monomer Me-D-Glc could be recovered in 34% yield. Despite the low yield, this result suggested the chemical recyclability of precision polysaccharides. Further thermodynamic analysis via variable-temperature .sup.1H NMR revealed the standard state thermodynamic parameters of the living CROP of Et-D-Glc: ?H.sup.o=?26.8 KJ/mol, ?S.sup.o=?73.1 J.Math.mol.sup.?1.Math.K.sup.?1. Correspondingly, the ceiling temperature (T.sub.c) was calculated to be 366 K (93? C.) at [M].sub.0=1.0 M, suggesting that the precision polysaccharide could be catalytically depolymerized under a relatively mild condition close to the T.sub.c. Consistent with this rationale, it was found that P(Et-D-Glc) could be quantitatively depolymerized to yield the monomer Et-D-Glc at 80? C. in the presence of a catalytic amount of BF.sub.3.Math.Et.sub.2O (
[0126] Table 3. M.sub.n vS [Et-D-Glc].sub.0:[I-3].sub.0 results. Experiments were performed according to general polymerization procedure, [Et-D-Glc].sub.0 was held constant and [I-3].sub.0 varied to achieve desired [Et-D-Glc].sub.0:[I-3].sub.0.
TABLE-US-00003 TABLE 3 Entry [M] [M].sub.0:[I].sub.0 Conversion M.sub.n.sub., .sub.Theo(kDa) M.sub.n.sub., .sub.NMR(kDa) M.sub.n.sub., .sub.GPC(kDa) ? [?].sub.D.sup.20 1 Et-D-Glc 25 75% 5.2 5.5 4.2 1.12 +171.6 2 Et-D-Glc 50 79% 10.2 10.4 6.7 1.13 +171.1 3 Et-D-Glc 71 75% 13.6 13.3 9.0 1.15 +169.7 4 Et-D-Glc 100 74% 18.0 18.7 13.3 1.17 +172.4
[0127] Table 4. Kinetic studies for [Et-D-Glc] polymerization. Experiments were performed according to general polymerization procedure, [Et-D-Glc].sub.0=1.5 M, [Et-D-Glc].sub.0:[I-3].sub.0=200. Time-point aliquots were taken during polymerization for .sup.1H NMR and SEC analyses.
TABLE-US-00004 TABLE 4 Entry [M] time Conversion M.sub.n.sub., .sub.Theo(kDa) M.sub.n.sub., .sub.NMR(kDa) M.sub.n.sub., .sub.GPC(kDa) ? 1 Et-D-Glc 0.5 h 27% 13.8 13.3 8.0 1.07 2 Et-D-Glc 1.0 h 48% 24.2 22.7 15.1 1.07 3 Et-D-Glc 1.5 h 59% 29.6 25.4 18.8 1.09 4 Et-D-Glc 2.0 h 65% 32.5 31.3 20.0 1.18 5 Et-D-Glc 2.5 h 74% 37.0 35.0 24.4 1.13
TABLE-US-00005 TABLE 5 Summary of [Et-D-Glc].sub.eq obtained by VT-NMR. Entry Temperature (? C.) Conversion (%) [Et-D-Glc].sub.eq (M) Rln[Et-D-Glc].sub.eq (J .Math. mol.sup.?1 .Math. K.sup.?1) 1/T (K.sup.?1) 1 25 94 0.090 ?20.0209 0.00335 2 33 92 0.120 ?17.6289 0.00327 3 40 88 0.180 ?14.2577 0.00314 4 50 85 0.225 ?12.4024 0.00305 5 60 78 0.330 ?9.2180 0.00296
Example 7
Material Properties
[0128] Labile main-chain groups (e.g., acetal, ester, enamine) are often incorporated into the existing chemically recyclable polymers to enable depolymerization.sup.20,34-38, rendering these polymers unstable under harsh conditions (e.g., strong acid/base, high temperature, etc.). In contrast, the precision polysaccharides exhibited remarkable chemical and thermal stability. Little to no change in molecular weight and dispersity was found when exposing P(Me-D-Glc) to an excessive amount of Br?nsted acid (e.g., acetic acid or trifluoroacetic acid) or base (e.g., pyridine, triethylamine, or 1,8-diazabicyclo[5.4.0]undec-7-ene) for 24 h (
[0129] The chemical recyclability, together with good chemical and thermal stabilities make these polysaccharides promising candidates for sustainable materials (
[0130] Table 6. Stress, strain, and Young's modulus values of tensile tests at a strain rate of 5 mm/min for PN1 and PN1 (sample 4).
TABLE-US-00006 TABLE 6 Sample Number Stress (MPa) Strain (%) E (MPa) 1 4.09 148 96.48 2 4.10 132 107.86 3 4.00 133 115.55 4 4.17 167 86.03
[0131] Table 7. Stress, strain, and Young's modulus values of tensile tests at a strain rate of 5 mm/min for PN2 and PN2 (sample 4).
TABLE-US-00007 TABLE 7 Sample Number Stress (MPa) Strain (%) E (GPa) 1 19.36 2.5 0.81 2 17.95 2.4 0.77 3 20.21 2.8 0.89 4 17.13 1.8 1.01
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[0188] It should be emphasized that the above-described embodiments of the present disclosure are merely possible examples of implementations set forth for a clear understanding of the principles of the disclosure. Many variations and modifications may be made to the above-described embodiment(s) without departing substantially from the spirit and principles of the disclosure. All such modifications and variations are intended to be included herein within the scope of this disclosure and protected by the following claims.