Coating metal oxide particles

Abstract

The invention relates to methods of forming coated metal oxide particles, suspensions of such coated particles, particles comprising functionalized surface coatings and to fortification of food crops with coated metal oxide particles. Embodiments disclosed include a method of fortifying a food crop with a trace element, the method comprising growing the food crop in a growth medium comprising the trace element in the form of metal oxide particles coated with an organic compound, and a food crop fortified with a trace element in the form of metal oxide particles. Also disclosed is a method of forming coated particles, the method comprising: providing a first quantity of metal oxide particles; providing a second quantity of a coating material comprising an organic compound; and mechanically mixing the metal oxide particles with the coating material in a dry mixing process to provide a mixture comprising the metal oxide particles coated with the organic compound.

Claims

1. A method of forming electrostatically coated particles for fortifying food crops or for drug or compound delivery into a body, the method comprising: providing a first quantity of dry, solvent-free metal oxide particles; providing a second quantity of a dry, solvent-free coating material comprising an organic compound; and mechanically mixing the metal oxide particles with the coating material in a dry, solvent-free mixing process to provide a mixture comprising the metal oxide particles electrostatically coated with the organic compound; wherein the mixing process is performed at a temperature of no more than 60 degrees C.

2. The method of claim 1 wherein the metal oxide particles comprise iron oxide.

3. The method of claim 1 wherein the mixing process comprises applying shear to mix the metal oxide particles and the coating material in a grinding process.

4. The method of claim 1 wherein the organic compound comprises an amino acid.

5. The method of claim 1 comprising a further step of dispersing the mixture in a solvent to provide a colloid of the electrostatically coated particles in the solvent.

6. The method of claim 5 comprising a further step of a wet mixing process to disperse the mixture in the solvent.

7. The method of claim 1 comprising a further step of dispersing the electrostatically coated particles in a polymer precursor followed by polymerisation of the polymer precursor.

8. The method of claim 1 wherein the electrostatically coated particles have a volumetric mean particle size of between 2 nm and 100 nm in diameter.

9. The method of claim 1 wherein the mixing process comprises applying shear to mix the metal oxide particles and the coating material in a milling process.

10. The method of claim 1 wherein the organic compound comprises a peptide.

11. The method of claim 1 wherein: the metal oxide particles comprise iron oxide; the mixing process comprises applying shear to mix the metal oxide particles and the coating material in a grinding or milling process; the method further comprises a step of dispersing the mixture in a solvent to provide a colloid of the electrostatically coated particles in the solvent; the method further comprises a wet mixing process to disperse the mixture in the solvent; and the method further comprises a step of dispersing the electrostatically coated particles in a polymer precursor followed by polymerisation of the polymer precursor.

12. A method of fortifying a food crop with a trace element, the method comprising: forming coated particles by: providing a first quantity of dry, solvent-free metal oxide particles; providing a second quantity of a dry, solvent-free coating material comprising an organic compound; and mechanically mixing the metal oxide particles with the coating material in a dry, solvent-free mixing process to provide a mixture comprising the metal oxide particles electrostatically coated with the organic compound wherein the dry, solvent-free mixing process is performed at a temperature of no more than 60 degrees C. and the metal oxide particles have a volumetric new particle size of between 2 nm and 100 nm in diameter; and subsequently growing the food crop in a growth medium comprising the coated particles.

13. The method of claim 12 wherein the trace element is iron in the form of iron oxide particles.

14. The method of claim 12 wherein the organic compound comprises an amino acid.

15. The method of claim 12 wherein the food crop is a tuberous, crop.

16. The method of claim 12 wherein the food crop is grown hydroponically, the metal oxide being provided as a suspension in a feed solution.

17. The method of claim 12 wherein the organic compound comprises a vitamin.

18. The method of claim 12 wherein the organic compound comprises a peptide.

19. The method of claim 12 wherein the organic compound comprises a pharmaceutical compound.

20. The method of claim 12 wherein the organic compound comprises an imaging agent.

Description

DETAILED DESCRIPTION

(1) Aspects and embodiments of the invention are described in further detail below by way of example and with reference to the enclosed drawings in which:

(2) FIG. 1 is a transmission electron micrograph of glutamic acid coated SPIO nanoparticles (scale bar=50 nm);

(3) FIG. 2 is a depth profile across a single nanoparticle in the micrograph of FIG. 1, indicating a nanoparticle diameter of around 8 nm;

(4) FIG. 3 is a transmission electron micrograph of vitamin C coated SPIO nanoparticles after autoclaving (the marker indicates 0.2 m);

(5) FIGS. 4a and 4b are plots of relaxation times T.sub.2 and T.sub.1 respectively for histidine coated SPIO nanoparticles diluted to 50 mg/L with distilled water;

(6) FIG. 5 is a schematic flow diagram illustrating a method according to an aspect of the invention;

(7) FIG. 6 is a simplified schematic diagram illustrating electrostatic bonding of organic molecules to a charged metal oxide particle;

(8) FIGS. 7a and 7b are a pair of T.sub.2 relaxation plots showing permeation of liposome-encapsulated SPIO particles coated with vitamin C through porcine skin over 0-40 minutes (FIG. 7a) and 0-4 hours (FIG. 7b).

(9) FIG. 8a is a dynamic light scattering plot showing the size distribution of iron oxide-tin oxide nanoparticles coated with fluorescent cell-penetrating peptide (RhodamineB-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-OH) in aqueous dispersion;

(10) FIG. 8b is a transmission electron micrograph of the same particles after sample preparation by evaporation on carbon-coated copper TEM grid;

(11) FIG. 8c is a TEM-EDX (energy dispersive X-ray spectroscopy) plot showing the elemental composition of these particles;

(12) FIG. 9 is a plot of average plant height for varying concentrations of iron oxide nanoparticles as a function of growth time;

(13) FIG. 10 is a plot of T.sub.2 relaxation time for tuber samples grown in solutions having varying levels of iron oxide nanoparticles;

(14) FIG. 11 is a plot of T.sub.2 relaxation time as a function of concentration of added iron oxide particles and for different growth times;

(15) FIGS. 12 and 13 are T.sub.2 and T.sub.1 relaxation times respectively for a feed solution after a complete growth cycle;

(16) FIG. 14 is a plot of average concentration of iron in the leaves of sample plants as a function of iron oxide particle concentration, for different growth times;

(17) FIG. 15 is a plot of average iron content in potato tubers as a function of iron oxide particle concentration, for different growth times;

(18) FIG. 16 is a plot of average starch content of potato tubers as a function of iron oxide particle concentration, for different growth times; and

(19) FIG. 17 is a combined plot of average starch content and iron content as a function of iron oxide particle concentration, for different growth times.

(20) The following provides a detailed description of specific exemplary embodiments relating to iron oxide nanoparticles functionalised with various organic compound coatings. Similar methods may however also apply to other metal oxide nanoparticulate materials.

(21) A suitable starting material used in each of the following exemplary embodiments is freshly prepared SPIO nanoparticles, prepared by methods that are described in more detail by Khalafalla et al. (Khalafalla, S. E.; Reimers, G. W., IEEE Trans. Magn., 1980, 16, 178), and by Kim et al. (Kim, E. H.; Lee, H. S.; Kwak, B. K.; Kim, B., J. Magn. Magn. Mater., 2005, 289, 328-330). In an exemplary embodiment, to FeCl.sub.2.4H.sub.2O (10 g, 0.05 mol) was added FeCl.sub.3.6H.sub.2O (24.3 g, 0.09 mol) both dissolved in distilled water (100 ml). NH.sub.3 solution (50 ml, 35%, 0.90 mol) was added dropwise with stirring (ca. 5 minutes) then sonicated in a sonic bath (1 hour). The mixture was filtered using a sintered funnel and washed (250 ml distilled water, 250 ml diethyl ether) and allowed to dry in air. This process resulted in 10.8 g of particles, giving a yield of 93.3%. The size of the resulting particles was measured to be around 8-10 nm by TEM when suspended in ethanol. The particles were of solid black appearance, and responded to magnetic stimulus.

(22) In a first set of examples, dry SPIO nanoparticles were mixed with an equal weight of an amino acid previously recrystallised from 4 M aqueous HCl. Recrystallisation from hydrochloric acid in general tends to increase the solubility of the resulting coating material in water. The amino acid was ground with the SPIO for 5 minutes in an agate mortar. The resultant homogeneous brown powder was then taken up into distilled water and passed through a 0.2 m pore microfilter. The concentration of SPIO in the solution was analysed by inductive coupled plasma (ICP). All solutions were diluted with distilled water to a concentration of 50 ppm of Fe. The T.sub.1 and T.sub.2 relaxation times were measured using a low field NMR method as described by Kim et al.

(23) For amino acids and peptides presenting aqueous solubility problems, the coating agent (2 g) was first dissolved in aqueous HCl (4 M, 20 ml) aided by vortex. Solvent was then removed in vacuo to yield a dry white crystalline powder. For amino acids around 10 mbar or less pressure and 60 C. was used. For peptides 40 C. and around 10 mbar or less was used or alternatively acetone was added and kept at 20 C. for 24 hours. The resulting mixture was then centrifuged (10 minutes, 3000 rpm), the supernatant removed and the solid product washed with diethyl ether (350 ml), followed by drying in air or by freeze-drying.

(24) In a specific example, 0.1 g of SPIO and 0.1 g of coating agent were ground in a mortar for 5 minutes. 2 ml of distilled water was then added to the mixture and further grinding carried out for 5 minutes. A further 3 ml of distilled water was then added and the mixture homogenised.

(25) To separate the coated SPIO nanoparticles from the coating agent dissolved in the solvent, size exclusion chromatography was carried out using Sephadex G-50 gel filtration medium (GE Healthcare Bio-Sciences AB). This process is suitable for single amino acid coatings, peptide coated particles being less suitable due to the particles becoming stuck on the column. For such larger coatings alternative methods such as those described in the above referenced publications.

(26) Table 1 below indicates the different values for T.sub.1 and T.sub.2 for SPIO nanoparticle suspensions with various different amino acid coatings. This data indicates that amino acids with basic side chains (R, H and K) tend to be more effective negative contrast agents, leading to shorter proton relaxation times. Amino acids with hydrophobic side chains tend to be less effective, having longer relaxation times. Those with side chains containing sulphur behaved in a manner that does not appear to fit any pattern. It is postulated that the sulphur atoms react to form disulphide bridges which cause excess aggregation leading to the SPIOs having different properties to those with other amino acids, thus affecting the T.sub.1 and T.sub.2 relaxation times.

(27) TABLE-US-00001 TABLE 1 A comparison of measured T.sub.1 and T.sub.2 NMR relaxation times for a range of amino acid coatings applied to SPIO nanoparticles (errors for tryptophan and histidine could not be collected, and errors for T.sub.1 of cysteine were out of the measurement range of the instrument, probably due to the formation of sulphur bridges). Amino Acid T.sub.1 (ms) T.sub.2 (ms) Hydrophobic, Non- polar Alanine (A) 62.0 9.5 Valine (V) 15.7 3.1 Leucine (L) 72.4 7.9 Isoleucine (I) 56.9 8.3 Proline (P) 16.5 3.7 Methionine (M) 1.9 1.0 Phenylalanine (F) 75.1 7.9 Tryptophan (W) 180.3 5.3 Hydrophilic, Polar Threonine (T) 31.0 7.0 Cysteine (C) 1871.0 1954.6 Asparagine (N) 124.7 7.3 Glycine (G) 92.9 7.4 Serine (S) 52.3 8.8 Glutamine (Q) 51.5 8.4 Tyrosine (Y) 65.9 4.5 Acidic Aspartic Acid (D) 35.8 5.5 Glutamic Acid (E) 18.2 3.9 Basic Lysine (K) 58.9 8.5 Arginine (R) 12.6 3.4 Histidine (H) 11.0 3.7

(28) This indicates that generally as T.sub.1 increases, so does T.sub.2. This holds true with the exception of tryptophan, which demonstrated an increase in T.sub.1 corresponding with a decrease in T.sub.2 relative to the other samples. This may have been due to the hydrophobic nature of the amino acid, confirmed by the observation in the sample of a significant amount of aggregation and sedimentation.

(29) The particle size of each type of coated SPIO was determined by dynamic light scattering (DLS) and by transmission electron microscopy (TEM). FIG. 1 is a transmission electron micrograph of SPIO nanoparticles coated with glutamic acid. FIG. 2 is a profile across one particle, where the x-axis is a linear trace across the particle in nm and the y axis indicates relative intensity. Analysis by transmission electron microscopy indicated that the particles were around 8-10 nm in diameter, with a fairly uniform coating of amino acid of between 0.5 and 1 nm in thickness. Analysis by DLS tended to be in general agreement with TEM analysis, although with larger particles identified by DLS, apparently due to the formation of aggregates. Most of the nanoparticles were observed as single units although some were found in clusters depending on the degree of aggregation, with aggregation of nanoparticles leading to larger particles of around 20 nm-60 nm.

(30) Thermogravimetric analysis (TGA) indicated that amino acid coverage of the particles varied from around 30% to around 90% by mass. It is postulated that this non-uniformity is due to the properties of the capping agents leading to multi-layering of the amino acid onto the SPIO particles, possibly a result of the mechanical mixing process. Further experimentation may determine whether this is the case, or if further modification of the method can lead to a greater uniformity of layer formation.

(31) In order to determine the stability of the particles, degradation studies were carried out over a period of several hours, the results of which are shown in FIGS. 4a and 4b. Over six hours (300 minutes), measurements for both T.sub.1 (FIG. 3a) and T.sub.2 (FIG. 3b) were taken with an interval of 1 second between measurements. It is clear from the graph that both T.sub.1 and T.sub.2 relaxation times start to increase over this period of time, presumably due to degradation of the coated SPIO nanoparticles. This increase is however small, representing only an increase of 10 ms over this time period for T.sub.1 and 0.8 ms for T.sub.2. It is thought that the loss of signal is due to the loss of coating from the nanoparticles as an equilibrium is gradually reached with material in solution. The particles were observed to remain in suspension for 1 month at room temperature, and relaxation times were observed to remain similar after two months. Similar results to those shown in FIGS. 3a and 3b were observed in blood.

(32) An aliquot of a 50 mg/L histidine-SPIO dispersion was evaporated to dryness under vacuum, and 24 hours later rehydrated with the same volume of distilled water. When the rehydrated amino acid-SPIO complex was tested it was found that the iron content was lower than before the dehydration, indicating that the process is not 100% efficient as not all of the complex becomes resuspended. However, the T.sub.1 and T.sub.2 values were around those expected for the new concentration of iron with a histidine coating. Additionally, a dry sample of SPIO particles coated with vitamin C was subjected to autoclave condition of 121 C. and 15 psi for 20 minutes. These particles were then successfully resuspended in aqueous dispersion, and TEM showed the particle morphology was maintained and had not become agglomerated.

(33) In a further exemplary embodiment, an organic coating agent, in this case palmitic acid, was mixed with an SPIO nanoparticle powder in the same manner as described above. The mixture was then taken up, i.e. dissolved and suspended, into chloroform. The result was a brown organic solution. In further alternative embodiments, sonication was applied to a mixture of SPIO with amino acid, which was found to be ineffective. Room temperature mixing of a mixture of SPIO with amino acid was found to be effective for aspartic acid, glutamic acid and threonine. Mixing at elevated temperatures of around 60 C. was also attempted, but was found to be ineffective. Heating a mixture of SPIO and amino acid to just below the decomposition temperature of the amino acid was found to be effective for all of the amino acids with the exception of phenylalanine, which may have been due to its hydrophobic nature. However, for all of these alternative embodiments there was observed a large amount of aggregation leading to long relaxation times and very large particles as measured by TEM and DLS. From these results it was concluded that the heating method was not preferable.

(34) In a further example, a 1:1 mass ratio of hexadecanediol and SPIO were ground together for 5 minutes using a mortar and pestle. The mixture was then taken up into chloroform, centrifuged and the supernatant filtered. Analysis by ICP indicated the presence of 151 mg/I of Fe in the sample, indicating that the hexadecanediol, and in particular the diol group, had bound to the SPIOs. Particle size analysis by DLS indicated some agglomeration but with a majority of particles around 80 nm.

(35) As an alternative to hand mixing by mortar and pestle, grinding was also carried out using a KitchenAid Artisan burr coffee grinder on mixtures of organic compounds with SPIO nanoparticles, using the grinder with the burrs at the closest setting to maximise the shearing action on the dry powder mixture. The mixture was passed through the grinder five times to yield a uniform powder. This powder was then solubilised in a solvent such as distilled water or another suitable solvent depending on the organic compound for further analysis.

(36) In a series of experiments to determine the effect of alternative dry mixing methods, 2.00 g of SPIO and 2.00 g of HCl salt of histidine were processed either using a pestle and mortar for 5 minutes or through a coffee grinder 5 times. In each case, 100 mg samples were taken, amounting to 10 samples for each method. 5 ml of water was added to each of these samples individually and then processed through 0.2 m pore filter. Samples were diluted 1 in 10 for analysis using ICP in order to determine the homogeneity of the powders produced. The results, summarised in table 2 below, indicate that the coffee grinder method produces a more homogeneous powder, as expected from a mechanically controlled process, with a mean of 55.5 mg/l Fe content and a standard deviation of 4.1. The mortar and pestle processed powders were fairly homogeneous with a mean of 100.3 mg/l of Fe and standard deviation of 11.3.

(37) There is a clear significant difference in the Fe content of the powders produced by the two different methods as assessed for example by the Mann Whitney U test and T-test. For the U test, U.sub.1=45 and U.sub.2=55, and for the T test, T=3.410.sup.10.

(38) TABLE-US-00002 TABLE 2 Experimental data comparing mortar and pestle mixing (P samples) with coffee grinding mixing (C samples). Original Sample ppm Fe ppm Fe P1 11.55 115.5 P2 8.424 84.24 P3 9.415 94.15 P4 11.64 116.4 P5 9.675 96.75 P6 9.812 98.12 P7 8.988 89.88 P8 9.127 91.27 P9 11.16 111.6 P10 10.51 105.1 C1 5.31 53.1 C2 4.862 48.62 C3 5.402 54.02 C4 5.059 50.59 C5 5.558 55.58 C6 5.699 56.99 C7 6.212 62.12 C8 5.608 56.08 C9 6.096 60.96 C10 5.677 56.77 Standard 11.27533 Deviation Mean 100.301 Standard 4.179338 Deviation Mean 55.483

(39) According to certain embodiments, coated metal oxide particles may be suspended in a polymer matrix, for example by suspending the coated particles in a liquid polymeric precursor material prior to polymerisation. Coating materials may be used that are soluble in the polymer matrix, particular examples being paracetamol and diclofenac, which are soluble in methyl methacrylate and may be used as coating materials for SPIOs. As an example, the particles and coating material may be mixed as described above to provide a dry or solvent-free mixture. The mixture is then taken up into the monomer precursor liquid. Methyl methacrylate is a preferred example. Palmitic acid is an example of a coating material which may be applied to SPIOs and then suspended in toluene, which is miscible with many polymeric matrices. As with other examples, the suspended particles may be filtered, or centrifuged, to remove large unbound particles.

(40) Using a polymeric matrix as the suspending medium, or solvent, once the coated particles are suspended then they will remain fixed in the matrix as it is polymerised. Methods using this technique have been trialled, as detailed above, using methyl methacrylate, which is used extensively for synthesising polymers.

(41) As described above, a liquid coating material may be used, a preferred example being glycerol, having OH groups that enable binding to the surface of the metal oxide particles. In an example experiment, 1 g of SPIOs and 1 g (0.79 ml) of glycerol were placed in a mortar and ground with a pestle for 5 minutes. 5 ml of water was then added to this mixture before processing through a 0.2 um microfilter. A brown solution (SPIOs coated with glycerol suspended in water) was produced.

(42) While not wishing to be bound by any particular hypothesis, it is postulated that the reason the dry mixing method described herein is effective is because the coating materials contain functional groups containing lone electron pairs which are able to donate electrons towards the particles, thus allowing these groups to associate or bind to iron oxide on the surface of the nanoparticles. This is supported by the evidence that aspartic acid and glutamic acid, both of which have two carboxyl groups containing a greater number of unbound electrons, appear to bind more readily than other amino acids. Since each have two carboxyl groups it is reasonable to assume that, in support of this hypothesis, it is the carboxylic acid groups that are responsible for the binding. The solutions from amino acids that were recrystallised remained stable for a period of 1 month, with those not recrystallised being stable for up to 3 months.

(43) A series of comparisons were made between existing coated SPIO nanoparticles and selected examples of coated nanoparticles made according to the methods described above. Table 3 below indicates a comparison between the NMR T.sub.1 and T.sub.2 relaxation times for a dextran-coated SPIO nanoparticle composition (known as Endoremm, available in Europe from Guerbet S. A.) and nanoparticles coated according to the methods described herein with the amino acid histidine and the peptide P53(108). As a baseline comparison, the relaxation times for water are also given.

(44) Baseline comparisons could alternatively be made with other NMR active nuclei, such as fluorine (which may be used in the form of a fluorocarbon gas such as hexafluoroethane, C.sub.2F.sub.6).

(45) Table 4 below provides a further comparison of relaxation times for histidine-coated SPIO nanoparticles formed according to the methods described herein at various different stages of processing. The relaxation times increase from the as-formed values by between 2 and 3 times after 43 days. The relaxation times increase further upon dehydration followed by re-dissolving, increasing T.sub.1 by around 20 times and T.sub.2 by around 6 times, suggesting that not all of the SPIO particles are taken back up into solution after dehydration and re-dissolving.

(46) TABLE-US-00003 TABLE 3 A comparison of NMR relaxation times with existing dextran coated nanoparticles. SPIO Coating T.sub.1 (ms) T.sub.2 (ms) Dextran (Endorem) 14.2662 3.0424 Histidine 9.4887 2.6079 P53(108) 5.7752 1.4897 Water ~2500 ~100

(47) TABLE-US-00004 TABLE 4 A comparison of NMR relaxation times for histidine-coated SPIO nanoparticles under different conditions. Condition T.sub.1 T.sub.2 Histidine Coated SPIO 3.3637 1.3399 Histidine Coated SPIO + 43 days 9.4887 2.6079 Histidine dehydrated and redissolved 75.4144 8.5825 Histidine diluted in PBS (Phosphate 1113.3641 14.0335 buffered saline solution)

(48) The following coating agents have been applied successfully to SPIO nanoparticles using the dry mixing method described herein: All 20 naturally occurring amino acids: Class I and Class II peptides; Fatty acids, including palmitic acid (taken up into organic solvents such as chloroform, toluene or polymer matrices); Antibiotics including Neomycin and Nitrofurantoin: X-Ray imaging agent iodipamide; Fluorescent markers such as fluorescein; Vitamins including ascorbic acid; and Pharmaceuticals including Diclofenac, Paracetamol and aspirin.

(49) In the case of iodipamide, this X-ray imaging agent could advantageously be used in combination with SPIO nanoparticles to provide a combined contrast imaging agent, with the iodipamide providing X-ray imaging contrast and the SPIO providing NMR imaging contrast.

(50) Shown in FIG. 5 is a schematic flow diagram of a method according to an embodiment of the invention. A first quantity of metal oxide nanoparticles 41 and a second quantity of a coating material 42 are brought together and subjected to mechanical mixing 43. After mixing, the resulting dry powder mixture 44 may be stored or can be prepared for use by dispersing the mixture in a solvent 45. The resulting dispersion or colloid may be subjected to further mixing 46 following dispersion, and further dilution 47. The dispersion may also be subjected to filtration 48, either with or without the further mixing or dilution steps.

(51) FIG. 6 illustrates schematically in simplified form the general principle of how an organic compound, or ligand, may bond electrostatically to a metal oxide particle 51 such as a nanoparticle via one particular mechanism, in this case employing carboxyl groups. Carboxyl groups 54 (i.e. having the structure COOH, disassociating to COO.sup. in solution) of each molecule 53 of the organic compound are attracted to charges on the outer surface 52 of the particle 51, thereby creating a particle that is coated, or functionalised, with a selected bridging group B. Similar mechanisms apply to other groups able to dissociate in solution such as hydroxyl, amine or phosphate groups.

(52) In conclusion, it has been demonstrated herein that potentially bioactive MRI traceable nanoparticles can be synthesised by means of a simple, solvent-free coating method. The resulting coated nanoparticle mixtures can be made readily soluble/dispersible in water or another solvent, and the resulting solution can be stable over extended periods, lending such dispersions to future development for applications in areas such as contrast agents for medical imaging.

(53) The following sections describe experiments carried out on fortification of a food crop, namely potato, with coated iron oxide particles made according to methods similar to or the same as those described above.

(54) A number of amino acid coatings were initially selected due to their ease of binding to the iron oxide nanoparticle and their high solubility in water. Coatings of Glutamic Acid (E), Glycine (G), Histidine (H) and Alanine (A) were selected for testing. The test consisted of submerging potato plantlets in solutions containing SPIOs coated with each amino acid and nutrients for six hours a day for 3 weeks. The concentration of Fe was kept constant at 2 mg/l for each coating system, as measured by ICP-OES (Inductively Coupled Plasma Optical Emission Spectroscopy). A control system containing just the micronutrient was also tested as a comparative study. The plantlets were then splutter coated with gold and the location and concentration of Fe in the roots of the plantlets was measured using SEM-EDX (scanning electron microscopy, energy dispersive X-ray spectroscopy.

(55) 1 ml of Histidine coated SPIOs were added to 4 ml of buffer solutions of differing pH. (pH=2, 4, and 5). The solutions were then placed onto an MRI MOUSE and their T.sub.1 and T.sub.2 relaxation signals measured every 30 minutes over a 24 hour period. After completion of one complete growth cycle, samples of the feed solution from The tanks were taken and their T.sub.1 and T.sub.2 relaxation signals were measured. The data was normalised for each starting concentration then compared.

(56) For preparation of the iron oxide particles, two solutions, one containing both Fe(II) (1.97 mol dm.sup.3) and Fe(III) (3.08 mol dm.sup.3) and the other containing NH.sub.3 solution, were pumped into a spinning disc reactor at 46 ml/min and 55 ml/min respectively. The resultant solution was then filtered and the solid washed once with ethyl acetate then twice with water. The resulting SPIOs were dried in a vacuum oven overnight at 40 C. and 0.1 mmHg (0.13 mbar). Once dried the SPIO's were then ground with equivalent mass of Histidine in a mortar and pestle, dissolved in distilled water. The solutions were centrifuged and the supernatant passed through a 0.02 m syringe filter. The SPIO solutions were then analysed by ICP to measure their iron content, then added to the hydroponic tanks to obtain the correct concentration.

(57) In order to test the hypothesis that iron oxide nanoparticles could be utilised to increase the iron concentration in plants, a system to grow plants in an environment that exposes them to coated iron oxide nanoparticles was developed. The plant targeted for enrichment was potatoes (solanum tubersum) due to their quick growth cycle and being a staple crop naturally low in iron.

(58) Growth conditions were as follows: 5 day seedling establishment Optimum air temp (light/dark): 20/16 C. Photosynthestic photon flux: 500-800 mol m.sup.2 s.sup.1 Photoperiod (light/dark): 12/12 hrs Duration of growth: 63-84 days

(59) A hydroponic system was used to enable a high degree of control over the crop growing conditions. The system consisted of a reservoir for holding the nutrient solution, which was pumped into a growth medium (clay pellets) at six hour intervals with a constant flow rate of 100 ml/min. A set amount of nutrient was added to the reservoir at the beginning of the plant growth cycle, as indicated in Table 1 below, with the levels monitored via conductivity readings and maintained by the addition of a refill solution (see Table 2 below) added when needed. The pH of the nutrient solution was monitored and kept constant through the addition of HNO.sub.3 and KOH to prevent biasing of results. Six hydroponic systems were used, each containing eight individual potato plants. Different concentrations of coated iron oxide nanoparticles were added to the reservoirs of each system (see Table 3 below). Each plantlet was wrapped in fiberglass then placed into the clay pellet media at a depth of 5 cm. The fiberglass was used to help anchor the plant and maintain a moist environment.

(60) Every two days the height and number of stems for each plant was recorded, and every week the conductivity of the tank solutions measured and nutrient solution added when needed. On completion of one full growth cycle each plant was removed from the system, divided into roots, tubers and leaves. Each component was then weighed, the roots and leaves dried by hanging in a well-ventilated room for 2 weeks then weighed again to obtain the biomass. The tubers were washed with distilled water to remove any residue then taken for analysis straight away without any drying.

(61) TABLE-US-00005 TABLE 1 Composition of start-up nutrient. Nutrients M.sub.r mol/l g/l KNO.sub.3 101.1032 0.0025 0.252758 Ca(NO.sub.3).sub.2 164.088 0.00 0.41022 MgSO.sub.4 120.366 0.001 0.120366 KH.sub.2PO.sub.4 136.086 0.0005 0.068043 H.sub.3BO.sub.3 61.83 9.50E06 0.000587385 MnCl.sub.24H.sub.20 197.9052 7.40E06 0.001464498 ZnSO.sub.47H.sub.2O 287.5799 9.60E07 0.000276077 CuSO.sub.45H.sub.2O 249.6861 5.20E07 0.000129837 FeCl.sub.36H.sub.2O 270.2957 5.00E05 0.013514784 (NH.sub.4).sub.6Mo.sub.7O.sub.24 1235.873 1.00E08 1.23587E05

(62) TABLE-US-00006 TABLE 2 Composition of refill nutrient. Nutrients M.sub.r mol/l g/l KNO.sub.3 101.1032 4.60E02 4.6507472 Ca(NO.sub.3).sub.2 164.088 0.01 1.969056 MgSO.sub.4 120.366 1.00E02 1.20366 KH.sub.2PO.sub.4 136.086 5.60E02 7.620816 H.sub.3BO.sub.3 61.83 1.23E04 0.00760509 MnCl.sub.24H20 197.9052 7.40E06 0.001464498 ZnSO.sub.47H2O 287.5799 1.25E05 0.003594749 CuSO.sub.45H.sub.2O 249.6861 6.80E06 0.001697865 FeCl.sub.36H.sub.2O 270.2957 1.34E04 0.036219621 (NH.sub.4)6Mo.sub.7O.sub.24 1235.873 1.30E07 0.000160663

(63) TABLE-US-00007 TABLE 3 Concentration of Fe added to hydroponic systems. Concentration of Fe in SPIOs System number (mg/l) 1 (control) 0 2 4 3 8 4 6 5 10 6 12

(64) Three tubers from each system were selected, individually weighed then placed in separated beakers containing 50 ml nitric acid. After one week 10 ml of the digested solution was taken from each sample and centrifuged for 20 min at 5 C. 1 ml of the supernatant liquids were pipetted into 100 ml volumetric flasks which were made up with distilled water. The iron concentration for each sample was then determined by ICP-OES analysis (see Table 4 below) using calibration standards of known concentration. The same process was undertaken on the leaves (see Table 5) also.

(65) TABLE-US-00008 TABLE 4 Results for the ICP of tubers Concentration of SPIO/ Concentration of Fe in mg/l Weight of potato/g sample/mg/l 0 56.5 0.543106814 0 60.023 0.477673034 0 54.24 0.52944677 2 29.79 0.7667198 2 21.226 0.532051984 2 23.46 0.5834565 4 63.283 0.727932931 4 40.723 0.514716368 4 47.58 0.6123025 8 49.48 0.633362267 8 46.61 0.544922282 8 47.11 0.589757213 10 43.39 0.665625176 10 30.67 0.576235819 10 32.64 0.73934673 12 32.12 0.749311017 12 28.8 0.620396841 12 29.1 0.73446793

(66) TABLE-US-00009 TABLE 5 Results for the ICP of leaves Concentration of SPIO/mg/l Concentration of Fe in leaves/mg/l 0 0.096783212 0 0.131991014 0 0.081605692 2 0.104911768 2 0.104409204 2 0.095542743 4 0.089893508 4 0.085751001 4 0.089489036 8 0.167328166 8 0.079586658 8 0.091180773 10 0.086170141 10 0.088076948 10 0.096664607 12 0.087535319 12 0.09425815 12 0.128639972

(67) Ten tubers from each different concentration were placed in a Bruker BioSpec small bore scanner and there T.sub.1 and T.sub.2 relaxations were measured and recorded.

(68) For starch analysis, 0.1-0.5 g of each sample potato was homogenized in hot 80% ethanol to removed sugars. The solution was centrifuged and the residue retained. The solid was then washed 3 times with hot ethanol until the washing give no colour when added to an Anthrone reagent (comprising 200 mg of Anthrone dissolved in 100 ml of ice cold 95% sulphuric acid). Water (5 ml) and 52% perchloric acid was added to the residue, which was left to stand at 0 C. overnight. The solutions were then centrifuged again and the supernatant retained. The extraction was repeated again and the supernatants for each samples pooled and made up to 100 ml in volumetric flasks. 0.1 ml of the supernatants were then pipetted into a conical flask and made up to 1 ml with water.

(69) Glucose standards were prepared by taking different volumes of the working standard (0.2, 0.4, 0.6, 0.8 and 1.0 mL) and diluting up to 1 ml. 4 ml of the Anthrone reagent was added to both the test samples and the standards, which were then boiled for 8 minutes. Once boiled, the samples were cooled in an ice bath. The intensity of the peak at 630 nm was measured (see Table 6) and compared to known standards.

(70) TABLE-US-00010 TABLE 6 Results for starch analysis of tubers. Concentration of SPIO/mg/l Intensity of Uv-vis peak at 630 nm 0 0.83079 0 1.00285 0 0.9361 4 1.186 4 0.9761 4 0.9848 6 1.05581 6 1.00764 6 0.84334 8 0.86615 8 0.8433 8 0.87186 10 0.93023 10 0.80433 10 0.8287 12 0.93023 12 0.80433 17 0.8287

(71) A number of amino acid coatings were selected due to their ease of binding to the iron oxide nanoparticle and their high solubility in water. The coatings: E, G, H and A were the first four of these coating systems to be screened in a preliminary test. This test consisted of submerging potatoes plantlets in solutions containing SPIOs coated with each amino acid and nutrients, for six hours a day for 3 weeks. The concentration of Fe was kept constant at 2 mg/I for each coating system, measured by ICP-OES (Inductively Coupled Plasma Optical Emission Spectroscopy). A control system containing just the micronutrient was also tested as a comparative study.

(72) ICP analysis showed the plantlet submerged in Histidine and Glutamic acid coated SPIOs contained a higher concentration of iron per mass then the control. SEM (Scanning Electron Microscopy) analysis showed a higher Fe concentration in the roots of the plantlets exposed to the Histidine coated SPIOs compared with the control plantlet and the glutamic acid coated SPIO treatment.

(73) TABLE-US-00011 TABLE 7 EDS elemental analysis of plantlets exposed to histidine-coated SPIOs Element Weight % Atomic % C 60.27 69.40 O 32.82 28.37 P 1.96 0.88 Ca 1.26 0.43 Fe 3.69 0.91

(74) TABLE-US-00012 TABLE 8 EDS elemental analysis of control plantlet Element Weight % Atomic % C 64.54 71.76 O 32.36 27.01 P 2.08 0.90 Ca 0.96 0.32 Fe 0.05 0.01

(75) Due to these results histidine was selected as the coating for the first hydroponic trial.

(76) SPIOs were prepared using the co-precipitation of ferrous and ferric salt solutions with aqueous ammonia solution in a spinning disc reactor. The main components of the SDR are: a rotating ridged disc with 100 mm diameter with controllable speed, a feed system capable of pumping solutions onto the centre of the disc and a glass walled housing for the disc, with drainage to facilitate recovery of the SPIO product. The solutions produced by processing through the SDR were filtered then washed to isolate the SPIO product. This solid was then dried under vacuum and coated with Histidine via grinding with a mortar and pestle. The concentration Fe in the coated-SPIOs was measured using ICP-OES.

(77) Investigation into the effect of disc rotation speed found that 2000 rpm was the optimum spin speed for the SDR. At this speed the nano-particles produced were 10 nm in diameter and very uniform (FIG. 13). Below this spin speed a secondary particle morphology, was observed, these were rod like structures (FIG. 14) with larger diameters, possibly the result of residual chlorine salts present in the reaction mixture. Spin speeds above 2000 rpm shown no further increase in particle size. Due to these results all SPIOs used in the following experiments were made at 2000 rpm.

(78) As plants release organic acids into the rhizosphere in order to facilitate uptake of minerals, to ascertain if these changes in pH would affect the stability of the iron oxide nanoparticles, known concentrations of coated SPIO was added to a number of buffer solutions and their T.sub.2 relaxation time was measured over a period of 24 hours. If the SPIOs coating were disrupted by the buffer solution the iron oxide would drop out of solution and an increase in relaxation time would be observed. Buffers with a pH of 2, 4 and 6 were selected as they are analogous to pH changes found around the roots of plants. The results showed no significant change in relaxation time in any of the pH ranges tested which suggests that the coated SPIOs are stable in the tested pH range.

(79) The coated SPIOs were added at different concentrations (4 ppm, 6 ppm, 8 ppm, 10 ppm, 12 ppm) to the feed tanks of the hydroponic systems. The height of each plant in the six tank systems was measured every two days. FIG. 6 shows the average plant height in each tank over time, indicating that increasing SPIO concentrations have a positive effect on the overall growth rate of the potato plants. This result implies that there must be some level of interaction between the coated-SPIOs and the plant. The plants showed no external signs of iron toxicity e.g. leaf discoloration even at the highest concentration which suggests the iron absorbed is being effectively stored. The measurements for the 8, 10 and 12 ppm tanks had to be aborted early, due to a pump malfunction damaging the plants and forcing early harvest. The tubers harvested increased in size as the coated SPIO concentration increased, which reinforces the hypothesis that there is a positive interaction between SPIO and plant. To investigate if any SPIO was observed in the tubers produced MRI experiments were conducted.

(80) FIG. 9 illustrates the effect of SPIO on potato plant growth rate. The results for C=0 represent the control tank, C=4 corresponds to 4 ppm of coated SPIO added, C=6 corresponds to 6 ppm of coated SPIO added, C=8 8 ppm of coated SPIO added, C=10 10 ppm of coated SPIO added, and C=12 12 ppm of coated SPIO added. The results show a positive correlation between SPIO concentration and average plant height. For results in excess of 44 days, the difference between average plant height for the control and C=6 concentrations is up to around 10 cm, or around 20%. For results around 28 days the difference for the control and C=10 concentrations is around 15 cm, or an increase of over 100% in height.

(81) To observe if any coated-SPIO were deposited in the tubers of the plant, 10 tubers from each concentration range were placed in a Bruker Bio spec small bore MRI machine and their T.sub.2 relaxation times were measured. The results, shown in FIG. 10, showed that no SPIO were observed for concentration ranges of 4 ppm 72 or 6 ppm 73. If the SPIOs were present in the potatoes the T.sub.2 relaxation time should have reduced compared to the control 71. However, measurements of the average T.sub.2 relaxation time of each concentration resulted in an increase in the relaxation signal observed. This is illustrated in FIG. 11, which shows the average standard deviation of T.sub.2 distributions as a function of added SPIO concentration, showing results for 60 days growth 81 and 40 days growth 82. This is thought to be caused by there being more loosely bound water in the tubers, possibly as a result of osmosis effects. Higher concentrations of Fe ions in the tuber may therefore result in the plant absorbing more water to dilute it.

(82) MRI mouse analysis of the tank solutions after complete growth cycles showed no SPIO in solution the only signal found being identified as water. T.sub.1 and T.sub.2 relaxation times for the solution are illustrated in FIGS. 12 and 13. This meant all the coated SPIO added to the tanks had either been absorbed by the plant or had decomposed to Fe.sup.+2 and Fe.sup.+3 ions. Since the pH testing mentioned above indicated that the coated SPIOs were not sensitive to changes in pH it is reasonable to hypothesise that other possible interactions must be responsible for the degradation of the SPIO coating, possibly the electrostatic attraction between the negatively charged SPIO surface and the positively charged root surface. To investigate if any of the iron from the SPIO had absorbed into the plant at all, iron content of the leaves and tubers of the potatoes plants from each concentration were measured.

(83) To analyse the Fe content of the leaves, three 5 g portions of leaves were removed from plants in each concentration range and digested in 50 ml of concentrated nitric acid over 5 days. The solutions were then centrifuged and 1 ml of the supernatant taken, diluted and the Fe concentration measured using ICP-OES. The results showed no change in the concentration of Fe with increasing concentration of coated-SPIO, as indicated in FIG. 14. This result was expected because Fe in the leaves is essential for photosynthesis and formation of chloroplasts and therefore its concentration is highly regulated in this location, so it was unlikely that any excess Fe would be stored there.

(84) The concentration of Fe in the tubers was tested in the same way as the leaves, ie by digestion of a known mass of potato in a known volume of acid. Testing began by utilising only cores samples of potatoes, however the results were inconclusive so whole potatoes were then used instead to gain more accurate concentration reading. These results, illustrated in FIG. 15, show a positive correlation between Fe concentration in the tuber and concentration of coated SPIO added to the feed. This suggests that the Fe from the SPIO is being transported into the plant and stored in the tubers. To investigate whether this increase of Fe in the tuber had any effect on the quality of the potato, testing into the starch content of the potatoes was also undertaken.

(85) Starch is the main energy store for a plant, and occurs in plants as water-insoluble granules. Starch granules contain two different sort of glucose polymers known as amylose and amylopectin. Starch is the most important carbohydrate used for food and feed purposes and represents the major resource for our diet. An increase in starch content in potatoes would therefore be potentially highly beneficial. To analysis the starch content of the potatoes, Anthrone reagent was used. The samples were washed with hot ethanol to remove sugars then digested in acid, diluted in water and then added to 4 ml of Anthrone reagent. The intensity of the peak at 630 nm was then recorded of each sample then the starch content calculated using calibration against a known series of glucose concentrations. The results of the test, shown in FIG. 16, indicate a positive correlation between the amount of starch present and an increasing coated SPIO concentration.

(86) FIG. 17 illustrates the correlation between iron concentration 171 (60 days), 172 (40 days) and starch content 173 (60 days), 174 (40 days) of potatoes grown according to the methods above as a function of iron content in solution. Both quantities increase with increasing coated SPIO concentration, suggesting that they may be related. While not wishing to be bound by any particular theory, this could be due to the fact that FeS clusters are used in the Oxidative phosphorylation metabolic pathway for the synthesis of adenosine triphosphate (ATP). In the plant ATP is converted into ADP by ATPases and ADP is a major component in the biosynthesis of starch. It can therefore be postulated that an increase in iron concentration in the tuber would result in an increased starch content. This higher starch content could explain the observed increase in tuber size.

(87) The results, as described above, indicate that iron oxide nanoparticles can be used to fortify potatoes through being incorporated via a growth medium. It is expected that the same or similar mechanism for fortification may be used for other trace elements such as selenium, magnesium or zinc, which may also be prepared in the form of nanoparticles coated with a suitable organic compound such as an amino acid. It has been shown that potato plants grown in the presence of a suspension of coated iron oxide particles results in an increase in the iron content of the resulting tubers, together with a surprising additional effect of an increase in the starch content of the tubers. The advantage of using iron oxide nanoparticles may therefore be not only in increasing the amount of iron in the food crop but also in the calorific quality of the food crop.

(88) Other embodiments are intentionally within the scope of the invention as defined by the appended claims.