Wolffia plant named ‘MANKAI’

Abstract

A new and distinct Wolffia Globosa plant that exhibits fast vegetative propagation, an ovoid shape, and is neutral in taste.

Claims

1. A new and distinct variety of Wolffia Globosa plant substantially as shown and described herein.

Description

DESCRIPTION OF THE DRAWINGS

(1) The accompanying photographic drawings illustrate the new cultivar, with the color being as nearly true as is possible with color illustrations of this type:

(2) FIG. 1 illustrates a population of plants of the new variety in log phase. The image was photographed with a Micros Cam 500 attached to a stereoscopic binocular microscope (Ladybird MZ1240) with lighting from above and below. The level of objective magnification is 45;

(3) FIG. 2 illustrates a non-synchronous population of plants in mid-log phase. 30 of 36 fronds are at some stage of budding, from incipient emergence of a daughter frond to almost complete separation of a mother and daughter frond. The size ranges of budding mother fronds are 0.6-0.8 mm in length and 0.5-0.6 mm in width. The variation in size is due to the growth cycle of a mother frond, which typically births 8-9 daughter fronds in its life time: a first-time mother reaches 0.6 mm in length, a third to eighth time mother reaches 0.8 mm in length. The widths are proportionate to the lengths. The image was photographed with a Nikon SMZ 1500 stereo microscope with lighting from above.

(4) FIG. 3 illustrates a single mature plant of the new variety.

(5) FIG. 4 illustrates a close up, dorsal view of a budding plant of the new variety with the dorsal face floating at the water line. The mother frond is birthing a daughter frond, with the meristematic pouch decorated with characteristic concentric lines of elongated cells at the mouth of the pouch. The image was photographed with a Nikon SMZ18 stereoscopic binocular microscope using a Nikon DS-RI1 camera with lighting from above. The magnification was provided by the 0.5 mm bar. The length of the mature mother frond is 0.8 mm and the width is 0.55 mm

(6) FIG. 5 illustrates a close up, side view of a budding plant of a mother frond of the new variety that reveals the green, photosynthetic, almost flat, dorsal face at the surface of the water and the diminishingly-green submerged body. The emerging daughter frond appears green throughout. The image was photographed with a Nikon SMZ18 stereoscopic binocular microscope using a Nikon DS-RI1 camera with lighting from above. The magnification was provided by the 0.5 mm bar. The length of the mature mother frond is 0.8 mm and the depth (height) is 0.7 mm.

(7) FIG. 6 illustrates a population of plants of the new variety, with one plant shown in side view. The image was photographed with a Micros Cam 500 attached to a stereoscopic binocular microscope Ladybird MZ1240 with lighting from above and below. The level of objective magnification is 45.

(8) FIG. 6a illustrates a new frond appearing from a mother frond of the new variety. The image was photographed with a Micros Cam 500 attached to a light microscope (Bresser Art. No. 57-23100). The level of objective magnification is 10.

(9) FIG. 7 illustrates a mother frond with a daughter frond of the new variety. The image was photographed with a Micros Cam 500 attached to a light microscope (Bresser Art. No. 57-23100). The level of objective magnification is 10.

(10) FIG. 8 illustrates a new frond of the present variety. The image was photographed with a Micros Cam 500 attached to a light microscope (Bresser Art. No. 57-23100). The level of objective magnification is 10.

(11) FIG. 9 illustrates young fronds of the new variety. The image was photographed with a Micros Cam 500 attached to a light microscope (Bresser Art. No. 57-23100). The level of objective magnification is 4.

(12) FIG. 9a illustrates an open pouch of the new variety. The image was photographed with a Micros Cam 500 attached to a light microscope (Bresser Art. No. 57-23100). The level of objective magnification is 10.

DESCRIPTION OF THE NEW VARIETY

(13) The following detailed description sets forth the characteristics of the new variety. The data which defines these characteristics are the result of asexual reproductions first carried out by budding in 2005 in Moshav Merhavia, Israel. The new variety was grown in a water medium at room temperatures under continuous low movement, with aeration, and fertilization provided. If the electrical conductivity was measured higher than 1.8 mS, the culture medium was refreshed. The location where the variety was described at an age of log stage growth was in Moshav Dekel, Israel under cool LED lighting with an intensity of 160 E m2 s1. Color references are primarily from the Pantone CatalogueThe Plus Series 2016.

PLANT

(14) Time to produce a finished plant: About 4-5 days at about 25 C. Description of the plant's multiplication habit: Fast vegetative propagation of 8 to 9 times over a period of 16 to 19 days before dying as a white frond devoid of any further daughter fronds within it. Body description: Frond without veins having one funnel-shaped budding pouch at the basal end. The clonal clusters are either solitary or two-connected. The dorsal surface is rounded on the edges with and the upper central portion is flattened. The floating variety shows only a central portion of its dorsal surface above water. No brown epidermal pigment cells are present. The new variety has a distinct translucent edge. Shape: Ovoid. Length: 0.4-0.85 mm. Diameter: 0.4-0.6 mm. Young plant color: Green (7490C). Mature plant color: Green (7490C). Stomata number: Less than 20. Flower description: None observed. Disease/pathogen resistance: None observed. Temperature tolerance: 30 C. of water media and 40 C. of the air. Drought tolerance: None. Use: As an addition to different kinds of food products in fresh, frozen, or dried form.

(15) The following TABLE 1 illustrates the biomass doubling time of the new variety:

(16) TABLE-US-00001 TABLE 1 BIOMASS DOUBLING SIZE COLOR TIME (Days) NOTES 0.5 mm Green 3-3.5 Green, 1 day lag before division. 0.6 mm Green 2-2.5 Green, small, rapid division. 0.8 mm Green 2-2.5 Green, full sized, rapid division. 0.8 mm Light green 3-5 Losing color, full sized, slower division. 0.9 mm None None Colorless, bloated size with age, no division.

(17) The nutritional value of the new variety is illustrated in the additional tables below:

(18) TABLE-US-00002 TABLE 2 General Composition % Protein 45.79 Ash 15.28 Carbohydrates 27.54 Moisture 4.9 Fat as Triglycerides 6.49 Saturated Fat 1.33 Polyunsaturated Fat 4.48 Monounsaturated Fat 0.38 Total Fatty Acids 6.2 Total Omega 3 isomers 3.46 Total Omega 6 isomers 1.01 Total Omega 9 isomers 0.08 Cholesterol 0 Dietary Fiber, total 23.3 Calories from Fat, 58 [kcal] Calories, [kcal] 352

(19) TABLE-US-00003 TABLE 3 Minerals mg/kg Calcium (Ca) 6410 Magnesium (Mg) 2440 Iron (Fe) 272 Sodium (Na) 1400 Phosphorus (P) 18400 Zinc (Zn) 158 Selenium (Se) 0.16

(20) TABLE-US-00004 TABLE 4 Amino Acids % Tryptophan 0.91 Cystine 0.48 Methionine 0.7 Aspartic acid 3.72 Threonine 1.85 Serine 1.8 Glutamic acid 4.47 Proline 1.77 Glycine 2.04 Alanine 2.39 Valine 2.27 Isoleucine 1.7 Leucine 3.28 Tyrosine 1.36 Phenylalanine 2.28 Total Lysine 3.06 Histidine 0.91 Arginine 4.2

(21) TABLE-US-00005 TABLE 5 Vitamins mg/100 g beta-carotene 45.41 Lutein 72.5 Zeaxanthin 13 Vitamin A (RAE) 3.785 Biotin (Vitamin 7) 0.087 Vitamin B1 (Thiamine HCl) 1.05 Vitamin B2 (Riboflavin) 3.24 Vitamin B3 (Niacin) 16.3 Vitamin B5 (Pantothenic Acid) 3.49 Vitamin C 99.4 Vitamin E (Tocopherols) 48.4 Vitamin K 0.293 Vitamin B6 (pyridoxine) 0.704 Folic acid, total 3.08 Choline, total 239