TISSUE SAMPLE CONTAINER AND METHODS

Abstract

A tissue sample container including a base having a plurality of sample holding sections, which are configured to receive a plurality of tissue samples in a given orientation and are demarcated by section walls; and a lid configured to sealingly engage the base. The sample holding sections are sized and shaped to correspond to a specific tissue sample size and shape such that the base in cooperation with the section walls, maintain the given orientation and identity of the tissue samples within respective sample holding sections.

Claims

1. A core biopsy tissue sample container comprising: a base having a sample holding section, which is configured to receive a plurality of biopsy core samples, and hold the plurality of biopsy core samples such that each of the biopsy core samples is substantially aligned with at least one adjacent biopsy core sample in a planar orientation, such that the plurality of biopsy core samples are non-overlapping when viewed from at least one angle; and a lid configured to engage and substantially seal the base.

2. The core biopsy tissue sample container according to claim 1, wherein the sample holding section comprises a plurality of sections, each section configured to receive one of the plurality of biopsy core samples.

3. The core biopsy tissue sample container according to claim 2, wherein the sample holding sections are differently sized and shaped to correspond to respective differently sized and shaped tissue samples.

4. A tissue sample container, comprising: one or more inserts having a plurality of sample holding sections, which are configured to receive a plurality of tissue samples in a given orientation, said sample holding sections being demarcated by section walls; a base configured to receive the one or more inserts that can be selectively placed in the base; and a lid configured to at least partially cover the sample holding sections and, in cooperation with the inserts, maintain the given orientation of the tissue samples within the respective sample holding sections, and sealingly engage the base herein the inserts are sized according to the tissue specific samples and selectively placed in the base.

5. The tissue sample container according to claim 4, wherein the inserts are removable such that the inserts are sized according to one of the tissue specific examples and can be placed into the tissue sample container to hold one of the tissue specific examples.

6. The tissue sample container according to claim 4, further comprising a fluid dispensing mechanism which is fluid communication with the inside of the tissue sample container for dispensing a fluid inside the tissue sample container while the lid and the base are engaged.

7. The tissue sample container according to claim 4, further comprising a label containing information identifying the tissue sample container and being, attached to at least one of the lid, the base, and one of the plurality of sample holding sections.

8. The tissue sample container according to claim 4, further comprising an identifier containing information identifying one of the plurality of tissue samples and being attached to at least one of the plurality of sample holding sections.

9. The tissue sample container according to claim 4, wherein the section walls prevent the plurality of tissue samples from passing between respective sample holding sections.

10. The tissue sample container according to claim 4, wherein the sample holding sections are differently sized and shaped to correspond to respective differently sized and shaped tissue samples.

11. A tissue sample container, comprising: a base having a plurality of sample holding sections, which are configured to receive a plurality of tissue samples in a given orientation, said sample holding sections being demarcated by boundary ribs; a retaining member configured to at least partially cover the sample holding sections and, in cooperation with the boundary ribs, maintain the given orientation of the tissue samples within the respective sample holding sections; and a lid configured to sealingly engage the base and in cooperation with the boundary ribs, maintain the given orientation and identity of the tissue samples within respective sample holding sections.

12. The tissue sample container according to claim 11, wherein a least one of the retaining member and the base is configured to urge against the plurality of tissue samples.

13. The tissue sample container according to claim 12, farther comprising a biasing element, wherein the retaining element is attached to the retaining member by the at least one biasing element configured to provide relative movement of the retaining element with respect to the retaining member.

14. The tissue sample container according to claim 13, wherein the retaining member comprises a plurality of retaining elements, each of the plurality of retaining elements configured to urge against one of the plurality of tissue samples.

15. The tissue sample container according to claim 11, wherein the sample holding sections are differently sized and shaped to correspond to respective differently sized and shaped tissue samples.

16. A tissue sample container, comprising: a base having a plurality of sample holding sections, which are configured to receive a plurality of tissue samples in a given orientation; a retaining member configured to at least partially cover the sample holding sections and, a lid configured to sealingly engage the base, wherein at least one of the base or the retaining member is configured, in cooperation with the other, to urge towards the plurality of tissue samples to maintain the given orientation of the tissue samples within the respective sample holding sections.

17. The tissue sample container according to claim 16, wherein the plurality of sample bolding sections of the base have a plurality of second tissue engaging surfaces which are configured to receive the plurality of tissue samples in the tissue sample container.

18. The tissue sample container according to claim 17, wherein the retaining member includes a frame portion and a retaining element attached to the frame portion by at least one biasing element configured to provide relative movement of the retaining element with respect to the frame portion, the retaining element having an first tissue engaging surface that is urged against the plurality of tissue samples such that the tissue samples are retained between the first tissue engaging surface and the plurality of second tissue receiving surfaces.

19. The tissue sample container according to claim 18, wherein the retaining member comprises a plurality of said retaining elements connected to the frame portion by respective biasing elements, each of the plurality of retaining elements corresponding to one of the plurality of tissue samples, wherein each of the plurality, of retaining elements are movable with respect to the frame portion independently of other retaining elements.

20. The tissue sample container according to claim 19, wherein the biasing element is substantially S-shaped including first and second end portions, one end portion being connected to the frame portion and the other end portion being connected to the tissue retaining element.

21. A tissue sample container according to claim 16, wherein the base has at least one mold having a second tissue engaging surface, which is configured to receive a plurality of tissue samples, wherein the retaining member includes a frame portion and a tissue retaining element attached to the frame portion by at least one biasing element configured to provide relative movement of the tissue retaining element with respect to the frame portion, the tissue retaining element having a first tissue engaging surface that is urged against the plurality of tissue samples such that the tissue samples are retained between the first tissue engaging surface and the second tissue engaging surface of the at least one mold of the base.

22. The tissue sample container according to claim 16, further comprising a fluid dispensing mechanism which is fluid communication with the inside of the tissue sample container for dispensing a fluid inside the tissue sample container while the lid and the base are engaged.

23. The tissue sample container according to claim 22, wherein said sample holding sections are in fluid communication with each other.

24. The tissue sample container according to claim 16, further comprising a label containing information identifying the tissue sample container and being attached to at least one of the lid, the base, and one of the plurality of sample holding sections.

25. The tissue sample container according to claim 16, further comprising a label containing information identifying one of the plurality of tissue samples and being attached to at least one of the plurality of sample holding sections.

27. A tissue sample container, comprising: a base having a plurality of sample holding sections, which are configured to receive a plurality of tissue samples in a given orientation, said sample holding sections being demarcated by boundary ribs; a retaining member configured to at least partially cover the sample holding sections and, in cooperation with the boundary ribs, maintain the given orientation of the tissue samples within the respective sample holding sections; and a lid configured to sealingly engage the base and in cooperation with the boundary ribs, maintain the given orientation and identity of the tissue samples within respective sample holding sections, wherein the retaining member further comprises a plurality of retaining elements each having a section perimeter, wherein the section perimeters are positioned over the boundary ribs to maintain the given orientation of the tissue samples within the respective sample holding sections.

28. The tissue sample container according to claim 27, further comprising a fluid dispensing mechanism which is in fluid communication with the inside of the tissue sample container for dispensing a fluid inside the tissue sample container while the lid and the base are engaged.

29. The tissue sample container according to claim 28, further comprising an identifier containing information identifying one of the plurality of tissue samples and being attached to at least one of the plurality of sample holding sections.

30. The tissue sample container according to claim 27, wherein the sample holding sections are differently sized and shaped to correspond to respective differently sized and shaped tissue samples.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0029] FIG. 1A illustrates a top view of a tissue sample container for according to a first example embodiment of the present application;

[0030] FIG. 1B illustrates a cross-section view of the tissue sample container for according to the first embodiment of the present application;

[0031] FIGS. 2A-2C show a base of the tissue sample container according to certain embodiments in a non-assembled state;

[0032] FIG. 3A is a cross-sectional view of one embodiment of the tissue sample container in an unassembled stated;

[0033] FIG. 3B is a cross-sectional view of the tissue sample container in FIG. 3A in an assembled state;

[0034] FIG. 4 is a planar view of the tissue sample container according to one embodiment;

[0035] FIG. 5A is an exploded view of a tissue sample container according to a first embodiment in a non-assembled state;

[0036] FIG. 5B shows the tissue sample container of FIG. 1A in an assembled state;

[0037] FIG. 6 is an exploded view of a tissue sample container according to another embodiment in a non-assembled state;

[0038] FIG. 7 is an exploded view of the tissue sample container according to one embodiment in a non-assembled state;

[0039] FIG. 8 is an exploded view of the tissue sample container according to one embodiment in a non-assembled state;

[0040] FIG. 9 shows a close-up view of the biasing element in a non-exemplary embodiment of the invention;

[0041] FIG. 10 is an exploded view of the tissue sample container according to one embodiment in a non-assembled state;

[0042] FIG. 11A is an exploded view of the tissue sample container according to one embodiment in a non-assembled state; and

[0043] FIG. 11B is a close-up view of the embodiment shown in 11A in an open state and a closed state.

DETAILED DESCRIPTION OF THE DRAWINGS

[0044] In the following detailed description, reference will be made to the accompanying drawing(s), in which similar elements are designated with similar numerals. The aforementioned accompanying drawings show by way of illustration and not by way of limitation, specific example embodiments and implementations consistent with principles of an example embodiment. These implementations are described in sufficient detail to enable those skilled in the art to practice an example embodiment and it is to be understood that other implementations may be utilized and that structural changes and/or substitutions of various elements may be made without departing from the scope and spirit of an example embodiment. The following detailed description is, therefore, not to be construed in a limited sense.

[0045] FIGS. 1A and 1B illustrate a first exemplary embodiment of the instant application. The tissue sample container 1 retains a tissue sample 2 in proper orientation to allow for the automation of the processing and a reduction of human error. As shown in FIG. 1B, a tissue sample container 1, according to one embodiment of the invention, has a base 4, a lid 8, and a sample holding portion 10 which cooperate to retain the tissue sample 2 in a particular orientation, as discussed below.

[0046] The base 4 has a bottom surface which may include a sample holding portion 10 and may be divided into a plurality of sample holding sections 11 demarcated by section walls 12 for holding the tissue samples 2 after they are obtained. The sample holding sections 11 may receive an individual sample (i.e. one sample in each section) or a plurality of samples in each section.

[0047] The tissue sample container 1 is configured to hold one or more samples 2. In the embodiment shown in FIGS. 1A and 1B, the tissue samples 2 are preferably core biopsy samples. In such instances, the tissue samples 2 may be elongated or otherwise shaped, such that the tissue samples 2 are unable to rotate in the sample holding sections 11 due to the engagement of the lid 8 in cooperation with the section walls 12. Thus, the sample holding sections 11 are configured in shape and size to receive a tissue sample and to maintain its orientation.

[0048] Further, when the lid 8 is placed on top of the base 4 the tissue sample container 1 is sealed as discussed in more detail below. Once the tissue sample container 1 is sealed, the section walls 12 in cooperation with the lid 8 sufficiently secures the tissue samples 2 (as shown in FIG. 1B) such that they maintain their orientation within the sample holding sections 11. Once sealed, the tissue sample container 1 can be transported without the tissue samples 2 losing their orientation.

[0049] The tissue sample holding sections are not limited to the elongated shape as shown in FIG. 1A. In fact, the size and shape of the tissue holding sections may be tissue specific. For example, the tissue holding sections 11 for core biopsy sample might be long and narrow, while the tissue holding sections 11 for skin tissue might be shorter and wider. FIGS. 2A-2C illustrate some additional shapes and sizes contemplated for the tissue sample holding sections 11 depending on the specific tissue contained in the tissue container 1. The shapes and sizes of the tissue sample sections 11 are not limited to these configurations. Also, the tissue sample container 1 may have one or more sample holding sections 11 each differently sized and shaped to receive a tissue sample 2 and retain the tissue sample 2 in a fixed orientation while the tissue sample 2 is in the container as shown in FIG. 2B.

[0050] For transport, the tissue sample container 1 is sufficiently sealed to secure the tissue samples 2 in a non-limiting embodiment. As shown in FIG. 1B the tissue sample container 1 may have a sealing gasket 16 (also illustrated in FIG. 5A). The sealing gasket 16 is not particularly limited and may be a rubber gasket, a plastic gasket, an O-ring, or any other sealing member as would be apparent to a person of ordinary skill in the art. In addition, the connection between the base 4 and the lid 8 is not particularly limited and may include one or more of a tongue and groove configuration, a threaded configuration, a snap fitting configuration, a pressure fitting configuration or any other configuration as would be apparent to a person of ordinary skill in the art.

[0051] An example of a sealing mechanism is shown in FIG. 1B. In this embodiment, the connection between the base 4 and the lid 8 may have a two-part closing system with a temporary closed position and a permanently closed position. FIG. 1B shows the permanently closed position. In the temporary closed position, the base 4 has a first lock 22 and second lock 24 and the lid 8 has a first snap 26 and a second snap 28. The first lock 22 and the first snap 26 engage to form the temporary closed position such that the samples may be held in a temporary locked position. In addition, the lid 8 can be further pressed onto the base 4 to a completely locked position such that the first lock 22 engages with the second snap 28 on the base 4 and the second lock 24 engages with the first snap 26 to form the permanently closed position. This permanently closed position forms a fixed closing and further creates a seal at the seal gasket 16. In a non-exemplary embodiment the lid 8 and the base 4 can only be removed by peeling away a part of the lid 8 to release the permanently closed position.

[0052] Alternatively, the lid 8 may be sealed to the base 8 as the lid 8 is made out of heat sealable film 29 (illustrated in FIG. 8). In this instance the heat sealable film 29 is attached to the base 4 in a heat sealable manner.

[0053] Once the tissue sample container 1 is sealed, it is ready for processing. As described above, a tissue sample 2 is processed with a fixing agent to fix and preserve the sample before analysis. The tissue sample container 1 may have fluid dispensing mechanism 30 or a means for processing the tissue samples 2 without disengaging the lid 8 from the base 4. Thus, formalin can be inserted at the top or bottom of the tissue sample container I and can penetrate around the tissue samples 2 even with the lid 8 and base 4 closed. This reduces the human exposure to formalin. The following is a description of different fluid dispending mechanisms 30 with reference to FIGS. 3A, 3B and 4.

[0054] As shown in FIG. 3A, with the fluid dispensing mechanism may include a pouch 34 containing a fluid 35 that is released into the tissue sample container 1 upon attachment of the lid 8 to the base 4. The fluid 35 in the pouch 34 may be any fluid to preserve and store any tissue samples during transportation, such as formalin. In the embodiment shown in FIGS. 3A-B, the pouch 34 is attached to the inside of the lid 8. The pouch 34 has a frangible portion 36 configured to be broken or ruptured to allow the fluid 35 be released into the tissue sample container 1. The pouch 34 and the frangible portion 36 are designed such that fluid 35 is released when the lid 8 becomes engaged with base 4.

[0055] More specifically, as shown in FIG. 3B, the pouch 34 is disposed directly above the tissue samples 2 and when the lid 8 is lowered onto the base 4, a squeezing force is applied to the pouch 34 as the lid 8 is forced downward. This motion increases the pressure of the fluid within the pouch 34 so that the frangible portion 36 ruptures when the pressure of the fluid 35 exceeds the strength of the frangible portion 36.

[0056] Thus, upon engagement of the lid 8 to the base 4, the frangible portion 36 breaks and releases the fluid 35 into the inside of the tissue sample container 1 as shown in FIG. 3B. The fluid 35 is then released into the sample holding sections 11 of the base 4, submerging the tissue samples 2 in the tissue sample container 1.

[0057] The frangible portion 36 may be a perforated region or a region formed of a material different from the remainder of the pouch 34, such that the frangible portion 36 of the pouch can be caused to break in a predictable manner. As an example, the lid 8 may be placed upside down to rest on top of the base 4 during transport to prevent inadvertent busting of the pouch 34. The volume of fluid 35 provided is chosen to be sufficient to fill the tissue sample container 1 such that the tissue samples 2 are submerged. Further, in some non-limiting embodiments, a breaking member having, for example, a needle shape and oriented to engage the frangible portion 36 of the pouch 34 and rupture the frangible portion 36 may be provided within the tissue sample container 1.

[0058] Alternatively, as shown in FIG. 4, in one embodiment of the invention, the fluid dispensing mechanism 30 may be a porthole 38 on the lid 8 of the tissue sample container 1. In this instance the porthole 38 may be attached to an external formalin reservoir (not shown in the figures) such that the formalin can be dispensed in the inside of the tissue sample container 1 through the porthole 38. While the porthole 38 is shown in the center of the lid 8, this location is not limiting and the porthole 38 could be located anywhere on the lid 8.

[0059] Further, the fluid 35 can pass in between the sample holding sections 10 depending on their size and height. For example in the embodiment shown in FIG. 2B, the section walls 12 are solid and high such that fluid 35 may not be able to pass through the section walls 12. However, in FIGS. 2A and 2C, the section walls are perforated such that fluid 35 may pass through the section walls 12.

[0060] Further, in some embodiments, a label 7 or ID tag, may be attached to each tissue sample container 1 as shown in FIG. 1A. Also an identifier 9 may be attached to one or more tissue holding sections 11 configured to receive the tissue sample 2. An important aspect of tissue transportation is properly keeping track of tissue samples. This includes not only tracking the tissue sample containers 1, but also easily identifying the samples 2 within a container.

[0061] In FIG. 1A, the label 7 is shown disposed on the base 4; however, the label 7 can be located anywhere on the tissue sample container 1. (An example of the label 7 on the lid 8 is shown in FIG. 7.)

[0062] In the embodiment shown in FIG. 1A, a single label 7 is present; however more than one label 7 may be present and the labels can be physically separated or located together. There also can be a label 7 for each tissue sample container 1 as well as for each tissue sample 2 in the tissue sample container 1. Thus, one or multiple labels 7 can be placed in the sample holding sections 11, the lid 8, the base 4, or another area of the tissue container 2.

[0063] In the embodiment shown in FIG. 1A, an identifier 9 is associated with each tissue holding section 11 to more easily identify the samples within the tissue container. Also, the identifiers 9 may help identify the orientation of the tissue sample 2. For example, the identifiers 9 as shown in FIG. 1A may provide guidance as to the north/south or left/right orientation of the tissue sample with respect to the identifier 9.

[0064] The label 7 or the identifier 9 may be a computer readable tag including, but not limited to, labels having an incorporated RFID, labels having an incorporated one-dimensional barcode (1-D barcode), labels having an incorporated two-dimensional barcode (2-D barcode), and labels having an incorporated three-dimensional barcode (3-D barcode). However, the computer readable label is not limited to RFID, 1-D barcode, 2-D barcode, or 3-D barcode labels and may include any type of label readable by a computer as would be apparent to a person of ordinary skill in the art.

[0065] In some embodiments, a label 7 or identifier 9 is present that may be sensitive to changes to the sample or to the tray itself. For example, the label 7 or identifier 9 may be present that changes physical (i.e. color) or chemical (i.e. redox, conjugation, etc.) properties during fixation of the sample. Similarly, a label 7 may be present that is sensitive to the processing steps which precede embedding (i.e. dehydration). Alternatively, the label 7 or identifier 9 present that is sensitive to the embedding step (i.e. infiltration of wax). The label 7 or identifier 9 may have a property that changes incrementally or switches when the step is complete. In this way, the technician, or an automated system, will be able to determine when the sample has finished one step before another is started.

[0066] The tissue sample container 1 of any embodiment of the present application may be formed from a variety of materials and their construction is not particularly limited. Further, an embodiment of this tissue sample container 1 may be constructed from a material having one or more of the following features: transparent on imaging or with minimal interference (i.e. radio transparent), resistant to chemical fixatives (such as formalin), resistant to degradation from chemicals used in tissue processing (such as alcohol, xylene or acids), and resistant to temperatures used in tissue embedding.

[0067] In addition, the lid 8 may include a coating on a surface which faces the tissues. The coating is such that is reduces the adhesion between the tissue sample and the tissue facing surface. In non-limiting embodiments, the coating can be a Teflon coating, including Polytetrafluoroethylene (PTFE) coating or the coating can be Polypropylene (PP).

[0068] In a second embodiment of the instant Application, the same base 4 may be used for a variety of differently sized and shaped sample holding sections 11. As such, the section walls 12 may be removable from the base 4 and the tissue sample container 1 may be used to retain the orientation of a variety of different tissue specific tissue samples.

[0069] In this embodiment an insert 13 (having the same shape as the outline of the section walls 12) is contemplated that may be inserted into the base 4 to form tissue specific, tissue sample holding sections 11. In this embodiment, a kit may be provided which has multiple inserts 13 with a variety of sized and shaped tissue specific sample holding sections 11. Each, tissue specific sample holding section 11 of the insert 13 may be shaped and sized to sufficiently maintain the orientation of the tissue specific tissue sample 2 in the tissue container 1. That is, the inserts 13 in cooperation with the lid 8 maintain the orientation of the tissue specific sample since they are shaped and sized according or the sample size of the tissue sample.

[0070] FIGS. 5A and 5B illustrate a tissue sample container 1 according to a second embodiment of the present application. The second embodiment mirrors the first embodiment with a few differences. First, a tissue sample container 1 according to a second embodiment of the application, includes a retaining member 6 which cooperates with the base 4 and the lid 8 to retain the tissue sample 2 in a particular orientation, as discussed above. Second, the tissue sample holding sections 11 are demarcated by boundary ribs 40 as opposed to section walls 12 in the above example. The boundary ribs 40, in an exemplary embodiment, are shorter in height than the section walls 12. As such, the boundary ribs 40 in cooperation with the retaining member 6 maintain the orientation of the tissue samples 2. In all other aspects the second embodiment is similar to the first embodiment.

[0071] The retaining member 6 is configured to be inserted over the base 4 as shown in FIG. 5A such that the retaining member 6 at least partially covers the sample holding sections 11. The retaining member 6 may have a single retaining element 14, as shown in FIG. 5A or a plurality of retaining elements 14, as shown in FIG. 6. In the example shown in FIG. 6, the retaining member 6 includes a plurality of retaining elements 14 corresponding in number and in alignment with the sample holding sections 11.

[0072] In an non-exemplary embodiment, each retaining element 14 has a mesh structure and is moveably attached to retaining member 6 by a biasing element 15. In this embodiment, there is a biasing element 15 on opposite sides of each retaining element 14. When the retaining member 6 is secured to the base 4, the biasing element 15 urges the retaining element 14 downwardly towards the sample holding sections 10. In one embodiment, the biasing element 15 urges the retaining elements 14 downwardlyagainst the tissue samples 2 so that the tissue samples 2 are retained between the retaining member 6 and the base 4 to maintain the proper orientation of the tissue samples 2.

[0073] Alternatively, the retaining elements 14 may urge against the boundary ribs 40 to provide a slight space between the tissue sample 2 and the retaining element 14 so that the retaining element 14 and the boundary ribs 40 cooperate to hold the tissue samples 2 in place. In such instances, the tissue samples 2 may be elongated or otherwise shaped, such that the tissue samples 2 are unable to rotate in the sample holding sections 10 due to the engagement of the boundary ribs 40 and the retaining element or elements 14.

[0074] Similar to the embodiment described above, the lid 8 is placed on top of the base 4 to seal the tissue sample container 1. Once the tissue sample container 1 is sealed, boundary ribs 2 in cooperation with the retaining element 14 sufficiently secures the tissue samples 2 such that they maintain their orientation within the sample holding sections 11, and the tissue sample container 1 can be transported without the tissue samples 2 losing their orientation.

[0075] FIG. 5A shows four sample holding sections 11; however, the sample holding sections are not limited to this number. Also, as an alternative to the above, the base 4 may incorporate the biasing element to bias a portion of the base against the tissue samples 2 to sufficiently hold the tissue samples' given orientation depending on the shape and size of the tissue sample 2. Further, the boundary ribs 40 are shown in FIG. 5A to extend in both the longitudinal and latitudinal direction of the base 4 such that the boundary ribs 40 cross to form substantially rectangular sample holding sections 11. The sample holding sections 11 are not limited to this shape and the boundary ribs 40 may extend in only one direction.

[0076] Similar to the embodiment above, the fluid 35 may be dispersed inside the container. The retaining member 6 may have a plurality of perforations 32 to allow the fixing agent to flow freely in the container 1. Thus, the fluid 35 released into the tissue sample container 1 can pass through the retaining member 6 to the tissue samples 2 in the sample holding sections 11. In the instances, where a porthole 38 is used for administering the fluid 35 the porthole 38 may also be connected through the retaining member 6, specifically in instances where the lid is a heat sealable film 29 as discussed above (illustrated in FIG. 7).

[0077] As an alternative example, the boundary ribs 40 may have perforated walls to further allow fluid to communicate between the sample holding sections 11. That is, in some instances the boundary ribs 40 may have a height great enough such that they partially or wholly contact the retaining member 6 or retaining elements 14 such that fluid 35 may be prevented from passing from one sample holding section 11 to another. Or, the boundary ribs 40 may have a very small height or have perforations to allow fluid flow between the sample holding sections 11.

[0078] Further, FIG. 7 illustrates another example of this embodiment where the boundary ribs have a small height such that they do not contact the retaining member 12 and the sample holding sections 10. In this instance, the retaining member 14 maintains the tissue samples fixed orientation and identity within the tissue sample container 1.

[0079] FIG. 8 shows an additional embodiment of the tissue sample container 1 of this application. This embodiment is the same as the embodiments described with respect to FIGS. 5A, 5B, 6, and 7 except in this embodiment, the base 4 or the retaining member 6 is configured, to urge towards the tissue samples 2 to clamp or engage the tissue samples. Similar to other embodiments, the retaining member 6 can contact the boundary ribs 40 and not urge directly against the tissue samples.

[0080] In the example shown in FIG. 8, the retaining member 6 includes a frame portion 48 and a plurality of retaining elements 14 corresponding in number and in alignment with the sample holding sections 11. In an non-exemplary embodiment, the retaining elements 14 have a mesh structure and are moveably attached to the frame portion 48 by a biasing element 15 respectively provided on opposite sides of each of the retaining elements 14. When the retaining member 6 is secured to the base 4, the biasing element 15 urges the retaining elements 14 downwardly away from the frame portion 48 and toward the sample holding sections 10. The bottom facing surfaces of the retaining elements 14 define a first tissue engaging surface 54 for respectively engaging the tissue sample 2. Further, the top surface of the base 4 defines a second tissue engaging surface 56 for receiving the tissue samples 2. The biasing element 15 urges the retaining elements 14 downwardly towards or against the tissue samples 2 so that the tissue samples 2 are retained between a first tissue engaging surface 54 of the retaining elements 14 and the second tissue engaging surface 56 of the base 4 to maintain the proper orientation of the tissue samples 2.

[0081] As shown in FIG. 8, according to this embodiment the retaining member 6 includes a plurality of retaining elements 14. However, the retaining member 6 may comprise only one retaining element 14, similar to the retaining member 6 as described with respect to FIG. 5A.

[0082] In addition, as shown in FIG. 8, the base 4 may comprise a plurality of molds 58 which receive the tissue sample 2. In this embodiment the sample 2 is retained between the surface of the mold 58 and the retaining elements 14.

[0083] As mentioned above, the retaining element 14 is attached to the frame portion 48 by a biasing element 15. That is, the biasing element 15 is attached at one end to the frame portion 48 and to the retaining element 14 on the other end. The biasing element 15 is configured to provide relative movement of the retaining element 14 with respect to the frame portion 48. The biasing element 15 urges the retaining element 14 towards the base 4 to maintain the tissue samples' orientation. Thus, the biasing element 15 can take on any shape that performs this function. Also in certain embodiments, the retaining elements 14 are movable with respect to the frame portion independently of other retaining elements 14.

[0084] One example of the biasing element 15 is shown in FIG. 9 where each biasing element has a substantially S or Z shape. In this example, each biasing element 15 has a first member 60 with a first end 62 and a second end 66. The first end 62 is connected to the tissue retaining element 14. Extending downward at an angle from the second end 66 of the first member 60 is a first angled member 64. A second angled member 68 is connected to the first angled member 64 by a first curved hinged point 74. The second angled member 68 extends upward from the first angled member 64 at an angle; and in a non-limiting embodiment, the second angled member 68 and the first angled member 64 form an angle less than 90. Extending downward from the second angled member 68 is a third angled member 70. The second angled member 68 and the third angled member 70 are connected by a second curved hinge point 76. In a non-limiting embodiment, the third angled member 70 and the second angled member 68 form an angle less than 90. Further, in a non-limiting embodiment, the third angled member 70 and the first angled member 64 form an angle less than 90. A second member 72 connects to the third angled member 70 and extends substantially parallel to the tissue retaining element 14. The second member 72 attaches to the retaining member 6 in a non-limiting embodiment.

[0085] FIG. 10 shows an alternate embodiment to the embodiments described with respect to FIGS. 1A, 1B and 2. This embodiment is similar to the embodiments described above, except in this embodiment, the retaining member 6 has retaining elements 14 which extend towards the lid 8 instead of towards the base 4. In this embodiment, the retaining elements 14 have section perimeters 44 which outline each retaining element 14. FIG. 11A illustrates an example where in the section perimeters 44 have a long and narrow shape.

[0086] As shown in FIG. 11A, the section perimeters 44 are positioned to partially cover the tissue samples 2 and to align over the boundary ribs 40 on the base 4. The section perimeters 44 can contact the boundary ribs 40 as shown in the close state of FIG. 11B, but they are not limited by this feature. In this example, the tissue sample 2 is sized according to the size of the retaining elements 14, such that the retaining elements 14, in combination with the boundary ribs 40, maintain the tissue sample's orientation.

[0087] Although a few example embodiments have been shown and described, these example embodiments are provided to convey the subject matter described herein to people who are familiar with this field. It should be understood that the subject matter described herein may be embodied in various forms without being limited to the described example embodiments. The subject matter described herein can be practiced without those specifically defined or described matters or with other or different elements or matters not described. It will be appreciated by those familiar with this field that changes may be made in these example embodiments without departing from the subject matter described herein as defined in the appended claims and their equivalents. Further, any description of structural arrangement of components or relationship there between is merely for explanation purposes and should be used to limit an example embodiment.

[0088] Aspects related to the example embodiment have been set forth in part in the description above, and in part should be apparent from the description, or may be learned by practice of embodiments of the application. Aspects of the example embodiment may be realized and attained using the elements and combinations of various elements and aspects particularly pointed out in the following detailed description and the appended claims.

[0089] It is to be understood that both the foregoing descriptions are an example and are explanatory only and are not intended to be limiting.