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DIRECT DETECTION METHOD FOR PATHOGENIC STRAIN HAVING RESISTANCE TO CARBAPENEM ANTIBIOTICS

20230059795 · 2023-02-23

    Inventors

    Cpc classification

    International classification

    Abstract

    The present invention relates to a method for detecting a pathogenic strain having resistance to carbapenem antibiotics in a biological sample. According to the present invention, it is possible to directly identify carbapenemases, specifically KPC, OXA, NDM, IMP, VIM and/or GES protein, by mass spectrometry, thereby making it possible to quickly determine not only whether a pathogenic strain has resistance to antibiotics, but also the type of protein involved in the resistance. According to the present invention, the physical and chemical properties of each carbapenemase in vivo, such as the unique N-terminal truncation length, methionine residue oxidation and disulfide bond formation in each type of carbapenemase, are identified and are reflected on reference mass values. Accordingly, it is possible to more closely detect the presence of an antibiotic-resistant strain with high reliability, and thus the present invention may be advantageously used to establish an appropriate strategy for antibiotic administration at an early stage of infection.

    Claims

    1. A method for detecting in a biological sample a pathogenic strain having resistance to carbapenem antibiotics, comprising: (a) isolating a protein expressed by a pathogenic strain in a biological sample isolated from a subject; and (b) performing top-down mass spectrometry on the isolated protein, wherein it is determined that the pathogenic strain having resistance to carbapenem antibiotics is present in the biological sample, when a protein having the same mass as Klebsiella pneumoniae carbapenemase (KPC) or OXA carbapenemase from which 21 or 22 amino acid residues at the N-terminus have been removed is detected as a result of the mass spectrometry or when a protein having the same mass as at least one carbapenemase selected from the group consisting of New Delhi Metallo-beta-lactamase NDM), imipenemase (IMP), Verona integron-borne metallo-β-lactamase (VIM) and Guiana extended spectrum β-lactamase (GES), from which 18, 19, 20, 21 or 26 amino acid residues at the N-terminus have been removed, is detected as a result of the mass spectrometry.

    2-8. (canceled)

    9. The method of claim 1, wherein the carbapenemase is a KPC protein, and it is determined that the pathogenic strain having resistance to carbapenem antibiotics is present in the biological sample, when a protein having the same mass as the KPC protein from which 21 amino acid residues at the N-terminus have been removed is detected as a result of the mass spectrometry.

    10. The method of claim 1, wherein the carbapenemase is an OXA protein, and it is determined that the pathogenic strain having resistance to carbapenem antibiotics is present in the biological sample, when a protein having the same mass as the OXA protein from which 22 amino acid residues at the N-terminus have been removed is detected as a result of the mass spectrometry.

    11. The method of claim 1, wherein the carbapenemase is an NDM protein, and it is determined that the pathogenic strain having resistance to carbapenem antibiotics is present in the biological sample, when a protein having the same mass as the NDM protein from which 19 or 20 amino acid residues at the N-terminus have been removed is detected as a result of the mass spectrometry.

    12. The method of claim 1, wherein the carbapenemase is an IMP protein, and it is determined that the pathogenic strain having resistance to carbapenem antibiotics is present in the biological sample, when a protein having the same mass as the IMP protein from which 18 to 21 amino acid residues at the N-terminus have been removed is detected as a result of the mass spectrometry.

    13. The method of claim 1, wherein the carbapenemase is a VIM protein, and it is determined that the pathogenic strain having resistance to carbapenem antibiotics is present in the biological sample, when a protein having the same mass as the VIM protein from which 25 or 26 amino acid residues at the N-terminus have been removed is detected as a result of the mass spectrometry.

    14. The method of claim 1, wherein the carbapenemase is a GES protein, and it is determined that the pathogenic strain having resistance to carbapenem antibiotics is present in the biological sample, when a protein having the same mass as the GES protein from which 18 amino acid residues at the N-terminus have been removed is detected as a result of the mass spectrometry.

    15. The method of claim 1, wherein it is determined that the pathogenic strain having resistance to carbapenem antibiotics is present in the biological sample, when one or more mass values (m/z×z) selected from the group consisting of 28720, 28746, 28737, 28780, 28678, 28728, 28704, 28806, 28736, 28738, 28688, 28562, 28877, 28676, 28686, 28733, 28690, 28557, 28718, 28963, 28716, 28588, 29105, 28795, 28825, 28769, 29631, 28760, 28656, 28776, 28975, 29086, 28748, 30433, 28730 and values within the ranges of these values±5 are detected as a result of the mass spectrometry.

    16. The method of claim 15, wherein the pathogenic strain having resistance to carbapenem antibiotics is a KPC protein-producing strain.

    17. The method of claim 15, wherein the mass values (m/z×z) additionally include a mass value that increased by 16 from each mass value.

    18. The method of claim 15 or 17, wherein the mass values (m/z×z) additionally include a mass value that decreased by 2 from each mass value.

    19. The method of claim 1, wherein it is determined that the pathogenic strain having resistance to carbapenem antibiotics is present in the biological sample, when one or more mass values (m/z×z) selected from the group consisting of 28147, 28098, 28117, 27679, 28172, 28158, 28282, 28032, 28048, 28252, 27673, 28190, 28260, 27718, 28126, 27900, 27653, 28002, 28175, 28149, 27877, 28161, 27955, 28151, 28131, 28215, 28191, 27978 and values within the ranges of these values±5 are detected as a result of the mass spectrometry.

    20. The method of claim 19, wherein the pathogenic strain having resistance to carbapenem antibiotics is an OXA protein-producing strain.

    21. The method of claim 19, wherein the mass values (m/z×z) additionally include a mass value that increased by 16, 32 or 48 from each mass value.

    22. The method of claim 1, wherein it is determined that the pathogenic strain having resistance to carbapenem antibiotics is present in the biological sample, when one or more mass values (m/z×z) selected from the group consisting of 26439, 26413, 26438, 26421, 26435, 26467, 26420, 26363, 26587, 26407, 26479, 26381, 26449, 26434, 27043, 26448, 26416, 26465, 26453, 26455, 26466, 26510, 26484, 26509, 26492, 26506, 26538, 26491, 26434, 26658, 26478, 26550, 26452, 26520, 26505, 27114, 26519, 26487, 26536, 26524, 26526, 26537 and values within the ranges of these values±5 are detected as a result of the mass spectrometry.

    23. The method of claim 22, wherein the pathogenic strain having resistance to carbapenem antibiotics is an NDM protein-producing strain.

    24. The method of claim 22, wherein the mass values (m/z×z) additionally include a mass value that increased by 16 or 32 from each mass value.

    25. The method of claim 22, wherein the mass values (m/z×z) additionally include a mass value that increased by 14, 28 or 42 from each mass value.

    26. The method of claim 22, wherein the mass values (m/z×z) additionally include a mass value that increased by 238 from each mass value.

    27. The method of claim 25, wherein the mass values (m/z×z) additionally include a mass value that increased by 238 from each mass value.

    28. The method of claim 1, wherein it is determined that the pathogenic strain having resistance to carbapenem antibiotics is present in the biological sample, when one or more mass values (m/z×z) selected from the group consisting of 25113, 25151, 25011, 25080, 25020, 25083, 25025, 24952, 25192, 25161, 24973, 24988, 25078, 25365, 25268, 25006, 25186, 24994, 25043, 24945, 25208, 25000, 24980, 25128, 25216, 24983, 25115, 25112, 25116, 25414, 25205, 25041, 25139, 25254, 25050, 24910, 25101, 25021, 25212, 25073, 24961, 25105, 24831, 25353, 25234, 24995, 25071, 25094, 25351, 25174, 25156, 25199, 25129, 24981, 25018, 25335, 25232, 24872, 24982, 25204, 24796, 25259, 25214, 25085, 25135, 25131, 25141, 25145, 25172, 25126, 24990 and values within the ranges of these values±5 are detected as a result of the mass spectrometry.

    29. The method of claim 28, wherein the pathogenic strain having resistance to carbapenem antibiotics is an IMP protein-producing strain.

    30. The method of claim 1, wherein it is determined that the pathogenic strain having resistance to carbapenem antibiotics is present in the biological sample, when one or more mass values (m/z×z) selected from the group consisting of 25322, 25515, 25488, 25391, 25339, 25516, 25464, 25485, 25527, 25531, 25414, 25455, 25421, 25499, 25542, 25129, 25405, 25534, 25446, 25472, 25444, 25298, 25338, 25367, 25355, 25508, 25264, 25306, 25336, 25352, 25543, 25407, 25268, 25419, 25514, 25501, 25487, 25445, 25341, 25364, 25424, 25458, 25491, 25513, 25348, 25518, 25350 and values within the ranges of these values±5 are detected as a result of the mass spectrometry.

    31. The method of claim 30, wherein the pathogenic strain having resistance to carbapenem antibiotics is a VIM protein-producing strain.

    32. The method of claim 1, wherein the pathogenic strain having resistance to carbapenem antibiotics is present in the biological sample, when one or more mass values (m/z×z) selected from the group consisting of 29217, 29274, 29186, 29216, 29247, 29246, 29259, 29203, 29231, 29201, 29273, 29261, 29237, 29248, 29230, 29213, 29275, 29278, 29221, 29194, 29338, 29232, 29227, 29251, 29202, 29175, 29369, 29661 and values within the ranges of these values±5 are detected as a result of the mass spectrometry.

    33. The method of claim 32, wherein the pathogenic strain having resistance to carbapenem antibiotics is a GES protein-producing strain.

    34. The method of claim 32, wherein the mass values (m/z×z) additionally include a mass value that increased by 16 or 32 from each mass value.

    35. The method of claim 32, wherein the mass values (m/z×z) additionally include a mass value that decreased by 2 from each mass value.

    Description

    BRIEF DESCRIPTION OF DRAWINGS

    [0077] FIG. 1A shows the results of SDS-PAGE analysis for the expression and sizes of KPC and OXA proteins derived from an antibiotic-resistant strain. FIG. 1B shows SDS-PAGE analysis results for the expression and sizes of three proteins (NDM, IMP and VIM) of the Metallo-β-Lactamase (hereinafter referred to as MBL) family, and the Class A carbapenemase GES protein. FIGS. 1C, 1D, 1E, 1F, 1G and 1H show the results of SDS-PAGE analysis for the expression and sizes of KPC, OXA, NDM, IMP, VIM and GES subtype proteins, respectively.

    [0078] FIGS. 2A, 2B, 2C, 2D and 2E show the results of SDS-PAGE analysis for the expression and sizes of KPC and OXA proteins derived from a clinical strain (FIG. 2A), the MBL proteins NDM (FIG. 2B), IMP (FIG. 2C) and VIM (FIG. 2D), and GES-5 protein (FIG. 2E).

    [0079] FIG. 3 shows the results of SDS-PAGE analysis for the expression and sizes of the MBL proteins depending on the concentration of zinc sulfate (ZnSO.sub.4).

    [0080] FIGS. 4A, 4B and 4C show the results of comparing the protein difference between a crude extract and a crude enzyme solution after sample pretreatment. For KPC and OXA proteins, cells were disrupted using each of a nonionic surfactant (FIG. 4A), an ionic surfactant (FIG. 4B) and a volatile buffer (FIG. 4C), the expression and solubility of each protein were analyzed by SDS-PAGE gel analysis. In addition, FIG. 4D shows the expression and solubility of the MBL protein in cells disrupted using a nonionic surfactant and FIG. 4E shows the expression and solubility of the GES protein in cells disrupted using with a volatile buffer, both as analyzed by SDS-PAGE gel analysis.

    [0081] FIGS. 5A, 5B, 5C, 5D, 5E and 5F show the results of separating and purifying target proteins using ion chromatography. KPC (FIG. 5A), OXA (FIG. 5B), NDM (FIG. 5C), IMP (FIG. 5D), VIM (FIG. 5E) and GES-5 (FIG. 5F) were separated and purified using an anion exchange resin column.

    [0082] FIGS. 6A, 6B, 6C, 6D, 6E and 6F show alignment results that comparatively show representative coverages (grey) for KPC protein, OXA protein, three MBL proteins, and GES protein, and oxidized methionine residues (bold and underlined).

    [0083] FIGS. 7A, 7B, 7C, 7D, 7E, 7F, 7G, 7H, 7I, 7J, 7K, 7L, 7M, 7N, 7O, 7P, 7Q, 7R, 7S and 7T show tandem spectrum results for peptides identified as N-terminal peptides in each protein. FIG. 7A shows the separation chromatogram of the KPC peptide, and FIG. 7B shows the results of identification of the N-terminal peptide of KPC. FIG. 7C shows the separation chromatogram of the OXA peptide, and FIG. 7D shows the results of identification of the N-terminal peptide of OXA. FIG. 7E shows an example of the separation chromatogram of the NDM peptide, and FIG. 7F shows the results of identification of the N-terminal peptide of NDM. FIG. 7G shows an example of the separation chromatogram of the IMP peptide, and FIG. 7H shows the results of identification of the N-terminal peptide of IMP. FIG. 7I shows an example of the separation chromatogram of the VIM peptide, and FIG. 7J shows the results of identification of the N-terminal peptide of VIM. FIG. 7K shows an example of the separation chromatogram of the GES peptide, and FIG. 7L shows the results of identification of the N-terminal peptide of GES. FIGS. 7M, 7N, 7O, 7P, 7Q, 7R, 7S and 7T show exemplary N-terminal identification results for representative subtypes of each peptide, and show the results of identification of the N-termini of KPC-3 (FIG. 7M), KPC-17 (FIG. 7N), OXA-181 (FIG. 7O), IMP-1 (FIG. 7P), IMP-4 (FIG. 7Q), VIM-1 (FIG. 7R), VIM-4 (FIG. 7S), and GES-1 (FIG. 7T), respectively.

    [0084] FIGS. 8A, 8B, 8C, 8D, 8E and 8F show examples of the results of multiple alignment analysis of the amino acid sequences of KPC (FIG. 8A), OXA (FIG. 8B), MBL (FIGS. 8C, 8D and 8E) proteins and GES protein (FIG. 8F), performed using the ClustalW program, the results of identification of the conservative amino acid sequences. Black boxes indicate N-terminal sequence regions.

    [0085] FIGS. 9A, 9B, 9C, 9D, 9E and 9F show phylogenetic analysis results for KPC (FIG. 9A), OXA (FIG. 9B), NDM (FIG. 9C), IMP (FIG. 9D), VIM (FIG. 9E) and GES (FIG. 9F) proteins.

    [0086] FIGS. 10A, 10B, 10C, 10D, 10E, 10F, 10G, 10H, 10I, 10J, 10K and 10L show the results of identification of KPC, OXA, NDM, IMP, VIM and GES proteins, performed using a high-resolution mass spectrometer. FIG. 10A shows the mass spectrum (monoisotopic mass: 28,700.69 m/z×z, average molecular weight: 28,718.13 m/z×z) of a multi-charged KPC protein, and FIG. 10B shows the tandem spectrum for KPC protein ions with a charge state of +17, and the identification result (E=1.8E-9) for the 22-293 a.a. sequence. FIG. 10C shows the mass spectrum (monoisotopic mass: 28,129.28 m/z×z, average molecular weight: 28,146.69 m/z×z) of a multi-charged OXA protein, and FIG. 10D shows the tandem spectrum for OXA protein ions with a charge state of +27, and the identification result (E=3.47E-42) for the 23-265 a.a. sequence. FIG. 10E shows the mass spectrum of a multi-charged NDM protein, and FIG. 10F shows the tandem spectrum for NDM protein ions with a charge state of the +25, and the identification result for the 20-270 a.a. sequence. FIG. 10G shows the mass spectrum of a multi-charged IMP protein, and FIG. 10H shows the tandem spectrum for IMP protein ions with a charge state of +30, and the identification result (E=2.99E-76) for the 19-246 a.a. sequence. FIG. 10I shows the mass spectrum of a multi-charged VIM protein, and FIG. 10J shows the tandem spectrum for VIM protein ions with a charge state of +20, and the identification result (E=4.76E-49) for the 27-266 a.a. sequence. FIG. 10K shows the mass spectrum of a multi-charged IMP protein, and FIG. 10L shows the tandem spectrum for GES protein ions with a charge state of +20, and the identification result (E=7.12E-8) for the 19-287 a.a. sequence.

    [0087] FIGS. 11A and 11B show the modified mass values of NDM protein, obtained by top-down mass spectrometry through high-resolution mass spectrometry. FIG. 11A shows the mass value of the palmitoylated protein type, and FIG. 11B shows the results for the methylated protein of the NDM protein and the resulting elution time difference.

    [0088] FIGS. 12A, 12B, 12C, 12D, 12E and 12F show sequence coverages for KPC (FIG. 12A), OXA (FIG. 12B), NDM (FIG. 12C), IMP (FIG. 12D), VIM (FIG. 12E) and GES (FIG. 12F) proteins, obtained by top-down mass spectrometry through a high-resolution mass spectrometer.

    [0089] FIGS. 13A, 13B, 13C, 13D, 13E and 13F show examples of mass spectrometry spectra of KPC protein (FIG. 13A), OXA protein (FIG. 13B), the MBL proteins NDM (FIG. 13C), IMP (FIG. 13D) and VIM (FIG. 13E), and GES protein (FIG. 13F), obtained using a low-resolution mass spectrometer (MALDI-TOF).

    MODE FOR INVENTION

    [0090] Hereinafter, the present invention will be described in more detail with reference to examples. These examples serve merely to illustrate the present invention in more detail, and it will be apparent to those skilled in the art that the scope of the present invention according to the subject matter of the present invention is not limited by these examples.

    EXAMPLES

    [0091] Experimental Methods

    Example 1. Cloning of β-Lactam Antibiotic Resistance Gene and Construction of Antibiotic-Resistant Strain

    [0092] Based on information about the gene sequences (European Molecular Biology Laboratory (EMBL) nucleotide sequence database accession numbers: KPC=CP026395.1, 882nt, OXA=AY236073.2, 798nt, NDM=CAZ39946.1, 813nt, VIM=AY884050.1, 801nt, IMP=AB616660.2, 741nt, GES=DQ236171.1, 864nt) obtained from the genes for carbapenemases, desired gene sequences were prepared by synthesis (Table 1). Using the synthetic genes, the following primers were prepared.

    TABLE-US-00001 1) KPC Primer 1: 5′-AACTGCAGGATGTCACTGTATCGCCGTCTA-3′ (30mer) Primer 2: 5′-GGAATTCTTACTGCCCGTTGACGCC-3′ (25mer) 2) OXA Primer 1: 5′-AACTGCAGGATGCGTGTATTAGCCTTATCGG-3′ (31mer) Primer 2: 5′-GGAATTCCTAGGGAATAATTTTTTCCTGTTTGA-3′ (33mer) 3) NDM Primer 1: 5′-AAC TGC AGG ATG GAA TTG CCC AAT ATT ATG CA-3′ (32mer) Primer 2: 5′-GGA ATT CTC AGC GCA GCT TGT CGG-3′ (24mer) 4) IMP Primer 1: 5′-AAC TGC AGG ATG AGC AAG TTA TCT GTA TTC TTT ATA T-3′ (37mer) Primer 2: 5′-GGA ATT CTT AGT TGC TTG GTT TTG ATG GTT TTT-3′ (33mer) 5) VIM Primer 1: 5′-AAC TGC AGG ATG TTC AAA CTT TTG AGT AAG TTA TTG-3′ (36mer) Primer 2: 5′-GGA ATT CCT ACT CAA CGA CTG AGC GAT T-3′ (28mer) 6) GES Primer 1: 5′-AAC TGC AGG ATG CGC TTC ATT CAC GCA CTA T-3′ (31mer) Primer 2: 5′-CGG AAT TCC TAT TTG TCC GTG CTC AGG AT-3′ (29mer)

    [0093] Restriction enzyme sites for cloning were added to the primers, and an open reading frame (ORF) was created to induce expression directly in a cloning vector.

    [0094] Each target gene was amplified by PCR from three types of template DNA. For PCR, a total of 50 μl of a PCR reaction solution was prepared using 3 μl of template DNA, 1.25 μl of 5′ primer, 1.25 μl of 3′ primer, 1 μl of dNTPs, 10 μl of 5× buffer, and 5×GC enhance buffer, and then PCR was performed under the following conditions: 1) denaturation—at 98° C. for 10 sec; 2) annealing—at 57° C. for 30 sec; 3) extension—at 72° C. for 30 sec. Cloning for construction of a recombinant plasmid containing each target gene was performed as follows: 1) For the insert gene and the vector, both ends of the DNA were cut into sticky ends using restriction enzymes, and 2) the insert gene was ligated into the vector using DNA ligase. 3) Thereafter, the vector was transformed into E. coli (E. coli Top10), and 4) recombinant E. coli was selected by the white/blue screening method. 5) A recombinant plasmid was extracted from the selected strain, and 6) the extracted plasmid was treated with restriction enzymes, and the DNA size was determined. 7) Finally, the inserted gene was confirmed through DNA sequencing.

    TABLE-US-00002 TABLE 1 Carbapenemases KPC ATGTCACTGTATCGCCGTCTAGTTCTGCTGTCTTGTCTCTCATGGCCGCTGGCTGGCTTTTCT GCCACCGCGCTGACCAACCTCGTCGCGGAACCATTCGCTAAACTCGAACAGGACTTTGGC GGCTCCATCGGTGTGTACGCGATGGATACCGGCTCAGGCGCAACTGTAAGTTACCGCGCTG AGGAGCGCTTCCCACTGTGCAGCTCATTCAAGGGCTTTCTTGCTGCCGCTGTGCTGGCTCG CAGCCAGCAGCAGGCCGGCTTGCTGGACACACCCATCCGTTACGGCAAAAATGCGCTGGT TCCGTGGTCACCCATCTCGGAAAAATATCTGACAACAGGCATGACGGTGGCGGAGCTGTC CGCGGCCGCCGTGCAATACAGTGATAACGCCGCCGCCAATTTGTTGCTGAAGGAGTTGGG CGGCCCGGCCGGGCTGACGGCCTTCATGCGCTCTATCGGCGATACCACGTTCCGTCTGGAC CGCTGGGAGCTGGAGCTGAACTCCGCCATCCCAGGCGATGCGCGCGATACCTCATCGCCG CGCGCCGTGACGGAAAGCTTACAAAAACTGACACTGGGCTCTGCACTGGCTGCGCCGCAG CGGCAGCAGTTTGTTGATTGGCTAAAGGGAAACACGACCGGCAACCACCGCATCCGCGCG GCGGTGCCGGCAGACTGGGCAGTCGGAGACAAAACCGGAACCTGCGGAGTGTATGGCACG GCAAATGACTATGCCGTCGTCTGGCCCACTGGGCGCGCACCTATTGTGTTGGCCGTCTACA CCCGGGCGCCTAACAAGGATGACAAGCACAGCGAGGCCGTCATCGCCGCTGCGGCTAGAC TCGCGCTCGAGGGATTGGGCGTCAACGGGCAGTAA OXA ATGCGTGTATTAGCCTTATCGGCTGTGTTTTTGGTGGCATCGATTATCGGAATGCCTGCGGT AGCAAAGGAATGGCAAGAAAACAAAAGTTGGAATGCTCACTTTACTGAACATAAATCACA GGGCGTAGTTGTGCTCTGGAATGAGAATAAGCAGCAAGGATTTACCAATAATCTTAAACG GGCGAACCAAGCATTTTTACCCGCATCTACCTTTAAAATTCCCAATAGCTTGATCGCCCTC GATTTGGGCGTGGTTAAGGATGAACACCAAGTCTTTAAGTGGGATGGACAGACGCGCGAT ATCGCCACTTGGAATCGCGATCATAATCTAATCACCGCGATGAAATATTCAGTTGTGCCTG TTTATCAAGAATTTGCCCGCCAAATTGGCGAGGCACGTATGAGCAAGATGCTACATGCTTT CGATTATGGTAATGAGGACATTTCGGGCAATGTAGACAGTTTCTGGCTCGACGGTGGTATT CGAATTTCGGCCACGGAGCAAATCAGCTTTTTAAGAAAGCTGTATCACAATAAGTTACACG TATCGGAGCGCAGCCAGCGTATTGTCAAACAAGCCATGCTGACCGAAGCCAATGGTGACT ATATTATTCGGGCTAAAACTGGATACTCGACTAGAATCGAACCTAAGATTGGCTGGTGGGT CGGTTGGGTTGAACTTGATGATAATGTGTGGTTTTTTGCGATGAATATGGATATGCCCACA TCGGATGGTTTAGGGCTGCGCCAAGCCATCACAAAAGAAGTGCTCAAACAGGAAAAAATT ATTCCCTAG NDM ATGGAATTGCCCAATATTATGCACCCGGTCGCGAAGCTGAGCACCGCATTAGCCGCTGCA TTGATGCTGAGCGGGTGCATGCCCGGTGAAATCCGCCCGACGATTGGCCAGCAAATGGAA ACTGGCGACCAACGGTTTGGCGATCTGGTTTTCCGCCAGCTCGCACCGAATGTCTGGCAG CACACTTCCTATCTCGACATGCCGGGTTTCGGGGCAGTCGCTTCCAACGGTTTGATCGTC AGGGATGGCGGCCGCGTGCTGGTGGTCGATACCGCCTGGACCGATGACCAGACCGCCCAG ATCCTCAACTGGATCAAGCAGGAGATCAACCTGCCGGTCGCGCTGGCGGTGGTGACTCAC GCGCATCAGGACAAGATGGGCGGTATGGACGCGCTGCATGCGGCGGGGATTGCGACTTAT GCCAATGCGTTGTCGAACCAGCTTGCCCCGCAAGAGGGGATGGTTGCGGCGCAACACAGC CTGACTTTCGCCGCCAATGGCTGGGTCGAACCAGCAACCGCGCCCAACTTTGGCCCGCTC AAGGTATTTTACCCCGGCCCCGGCCACACCAGTGACAATATCACCGTTGGGATCGACGGC ACCGACATCGCTTTTGGTGGCTGCCTGATCAAGGACAGCAAGGCCAAGTCGCTCGGCAAT CTCGGTGATGCCGACACTGAGCACTACGCCGCGTCAGCGCGCGCGTTTGGTGCGGCGTTC CCCAAGGCCAGCATGATCGTGATGAGCCATTCCGCCCCCGATAGCCGCGCCGCAATCACT CATACGGCCCGCATGGCCGACAAGCTGCGCTGA IMP ATGAGCAAGTTATCTGTATTCTTTATATTTTTGTTTTGCAGCATTGCTACCGCAGCAGAG TCTTTGCCAGATTTAAAAATTGAAAAGCTTGATGAAGGCGTTTATGTTCATACTTCGTTT GAAGAAGTTAACGGGTGGGGCGTTGTTCCTAAACATGGTTTGGTGGTTCTTGTAAATGCT GAGGCTTACCTAATTGACACTCCATTTACGGCTAAAGATACTGAAAAGTTAGTCACTTGG TTTGTGGAGCGTGGCTATAAAATAAAAGGCAGCATTTCCTCTCATTTTCATAGCGACAGC ACGGGCGGAATAGAGTGGCTTAATTCTCGATCTATCCCCACGTATGCATCTGAATTAACA AATGAACTGCTTAAAAAAGACGGTAAGGTTCAAGCCACAAATTCATTTAGCGGAGTTAAC TATTGGCTAGTTAAAAATAAAATTGAAGTTTTTTATCCAGGCCCGGGACACACTCCAGAT AACGTAGTGGTTTGGTTGCCTGAAAGGAAAATATTATTCGGTGGTTGTTTTATTAAACCG TACGGTTTAGGCAATTTGGGTGACGCAAATATAGAAGCTTGGCCAAAGTCCGCCAAATTA TTAAAGTCCAAATATGGTAAGGCAAAACTGGTTGTTCCAGGTCACAGTGAAGTTGGAGAC GCATCACTCTTGAAACTTACATTAGAGCAGGCGGTTAAAGGGTTAAACGAAAGTAAAAAA CCATCAAAACCAAGCAACTAA VIM ATGTTCAAACTTTTGAGTAAGTTATTGGTCTATTTGACCGCGTCTATCATGGCTATTGCG AGTCCGCTCGCTTTTTCCGTAGATTCTAGCGGTGAGTATCCGACAGTCAGCGAAATTCCG GTCGGGGAGGTCCGGCTTTACCAGATTGCCGATGGTGTTTGGTCGCATATCGCAACGCAG TCGTTTGATGGCGCAGTCTACCCGTCCAATGGTCTCATTGTCCGTGATGGTGATGAGTTG CTTTTGATTGATACAGCGTGGGGTGCGAAAAACACAGCGGCACTTCTCGCGGAGATTGAG AAGCAAATTGGACTTCCTGTAACGCGTGCAGTCTCCACGCACTTTCATGACGACCGCGTC GGCGGCGTTGATGTCCTTCGGGCGGCTGGGGTGGCAACGTACGCATCACCGTCGACACGC CGGCTAGCCGAGGTAGAGGGGAACGAGATTCCCACGCACTCTCTAGAAGGACTCTCATCG AGCGGGGACGCAGTGCGCTTCGGTCCAGTAGAACTCTTCTATCCTGGTGCTGCGCATTCG ACCGACAACTTAGTTGTGTACGTCCCGTCTGCGAGTGTGCTCTATGGTGGTTGTGCGATT TATGAGTTGTCACGCACGTCTGCGGGGAACGTGGCCGATGCCGATCTGGCTGAATGGCCC ACCTCCATTGAGCGGATTCAACAACACTACCCGGAAGCACAGTTCGTCATTCCGGGGCAC GGCCTGCCGGGCGGTCTAGACTTGCTCAAGCACACAACGAATGTTGTAAAAGCGCACACA AATCGCTCAGTCGTTGAGTAG GES ATGCGCTTCATTCACGCACTATTACTGGCAGGGATCGCTCACTCTGCATATGCGTCGGAA AAATTAACCTTCAAGACCGATCTTGAGAAGCTAGAGCGCGAAAAAGCAGCTCAGATCGGT GTTGCGATCGTCGATCCCCAAGGAGAGATCGTCGCGGGCCACCGAATGGCGCAGCGTTTT GCAATGTGCTCAACGTTCAAGTTTCCGCTAGCCGCGCTGGTCTTTGAAAGAATTGACTCA GGCACCGAGCGGGGGGATCGAAAACTTTCATATGGGCCGGACATGATCGTCGAATGGTCT CCTGCCACGGAGCGGTTTCTAGCATCGGGACACATGACGGTTCTCGAGGCAGCGCAAGCT GCGGTGCAGCTTAGCGACAATGGGGCTACTAACCTCTTACTGAGAGAAATTGGCGGACCT GCTGCAATGACGCAGTATTTTCGTAAAATTGGCGACTCTGTGAGTCGGCTAGACCGGAAA GAGCCGGAGATGAGCGACAACACACCTGGCGACCTCAGAGATACAACTACGCCTATTGCT ATGGCACGTACTGTGGCTAAAGTCCTCTATGGCGGCGCACTGACGTCCACCTCGACCCAC ACCATTGAGAGGTGGCTGATCGGAAACCAAACGGGAGACGCGACACTACGAGCGGGTTTT CCTAAAGATTGGGTTGTTGGAGAGAAAACTGGTACCTGCGCCAACGGGGGCCGGAACGAC ATTGGTTTTTTTAAAGCCCAGGAGAGAGATTACGCTGTAGCGGTGTATACAACGGCCCCG AAACTATCGGCCGTAGAACGTGACGAATTAGTTGCCTCTGTCGGTCAAGTTATTACACAA CTCATCCTGAGCACGGACAAATAG

    Example 2. Analysis of Expression and Size of Each Target Protein

    [0095] (1) Target Protein Production and Identification

    [0096] E. coli transformed with the plasmid containing each target gene was inoculated into Luria-bertani liquid medium containing 50 mg/L of ampicillin antibiotic, and cultured at 37° C. for 16 hours or more. In order to analyze the expression and size of each target protein, the culture was centrifuged at 4,000 rpm for 15 minutes, and the cells were harvested by removing the supernatant. The harvested cells were added to SDS-sample buffer, heated at 95° C. for 5 min, and centrifuged at 15,000 rpm for 5 min. Using the prepared samples, the expression and size of each target protein were analyzed through SDS-PAGE gel analysis (FIGS. 1A, 1B, 1C, 1D, 1E, 1F, 1G and 1H).

    [0097] (2) Confirmation of Genotypes of KPC and OXA Derived from Clinical Strain and Confirmation of Proteins

    [0098] In order to confirm KPC and OXA derived from a clinical strain, a strain confirmed as positive for extended spectrum β-lactamase (ESBL) was collected. The collected strain was subjected to colony PCR using the primers used for KPC and OXA gene amplification. The resulting amplified PCR products were subjected to agar gel electrophoresis to confirm the sizes of the target genes. The PCR products whose sizes have been confirmed were subjected to DNA sequencing to confirm the exact genotypes of the KPC and OXA genes.

    [0099] The strain whose genotypes have been confirmed was cultured in LB liquid medium, and SDS-PAGE gel analysis was performed to examine whether KPC and OXA proteins would be expressed. In addition, a recombinant strain containing the KPC and OXA genes derived from the clinical strain was constructed in the same manner as the existing recombinant strain comprising the vector containing the KPC and OXA genes, and the recombinant strain was also subjected to SDS-PAGE gel analysis in the same manner as the clinical strain to confirm the sizes of the actually expressed KPC and OXA proteins (FIGS. 2A, 2B, 2C, 2D and 2E).

    [0100] Clinical strain-derived KPC and OXA proteins were each identified through the Q-TOF MS method. For KPC, it was confirmed that the sequence coverage for the identified peptides was 62.46% (183/293) in the full-length protein sequence and 67.28% (183/272) in the active form excluding the N-terminus. In addition, for OXA, it was confirmed that the sequence coverage for the identified peptides was 16.98% (45/265) in the full-length protein amino acid sequence and 18.52% (45/243) in the sequence of the active form.

    [0101] (3) MBL Protein Production and Identification

    [0102] E. coli transformed with the plasmid containing the target MBL gene was inoculated into Luria-bertani liquid medium containing 50 mg/L of ampicillin antibiotic and zinc sulfate (0.01 mM to 1 mM ZnSO.sub.4), and cultured at 37° C. for 16 hours or more. In order to analyze the expression and size of the target protein, the culture was centrifuged at 4,000 rpm for 15 minutes, and the cells were harvested by removing the supernatant. The harvested cells were added to SDS-sample buffer, heated at 95° C. for 5 min, and centrifuged at 15,000 rpm for 5 min. Using the prepared sample, the expression and size of the target protein were analyzed through SDS-PAGE gel analysis (FIG. 3).

    Example 3. Sample Pretreatment Method and Identification of Target Protein from Crude Enzyme Solution

    [0103] (1) Sample Pretreatment with Nonionic Surfactant

    [0104] For sample pretreatment, the culture was centrifuged at 4,000 rpm for 15 minutes, and the cells were harvested by removing the supernatant. To obtain a crude extract, the cells were treated with a buffer solution (0.25 mM Tris-HCl, 2% OG) and incubated at room temperature for 10 minutes. The prepared crude extract was separated into a supernatant (hereinafter referred to as crude enzyme solution) and a precipitate by centrifugation at 15,000 rpm at 4° C. for 10 minutes. From the crude enzyme solution, the expression and size of the target protein were analyzed by SDS-PAGE analysis (FIG. 4A).

    [0105] (2) Sample Pretreatment with Ionic Surfactant

    [0106] Sample pretreatment was performed by the following steps: 1) 100 ml of the expressed cell culture was centrifuged and to recover the cells, and 2) the supernatant was removed, and then a buffer solution (0.25 mM Tris-HCl, pH 8.0 and 2% DOC) containing 2% sodium deoxycholate (DOC) as a nonionic surfactant was added to the cells. 3) The suspension was incubated at room temperature for 10 minutes, and 4) centrifuged at 15,000 rpm at 4° C. for 10 minutes to obtain a crude enzyme solution.

    [0107] From the crude extract and the crude enzyme solution, obtained by treatment with the ionic surfactant (DOC), the expression and size of the protein were analyzed by SDS-PAGE gel analysis (FIG. 4B).

    [0108] (3) Sample Pretreatment with Volatile Buffer

    [0109] The sample pretreatment method is as follows: 1) 50 mM ammonium bicarbonate (hereinafter referred to as ABC) solution was added to the harvested cells, 2) the cells were resuspended by pipetting, and then 3) the suspension was centrifuged at 12,000 rpm and 4° C. for 10 minutes to recover the supernatant. From the crude extract and crude enzyme solution obtained by treatment, the expression and size of the protein were analyzed by SDS-PAGE gel analysis (FIGS. 4C and 4E).

    [0110] (4) Sample Pretreatment by Sonication

    [0111] The cells in the suspensions treated by the methods (1) to (3) above were disrupted by sonication using a Sonic bath (JAC 2010, Hansol Tech, Korea) at 40 Hz and 200 W for 5 to 10 minutes, and centrifuged at 15,000 rpm for 10 min, and the supernatant was recovered.

    Example 4. Separation/Purification of Target Protein

    [0112] Ion exchange chromatography was used to separate/purify each target protein. For anion exchange chromatography, a column containing Q-resin was used, and for cation exchange chromatography, a column containing SP-resin was used.

    [0113] (1) Anion Exchange Resin Chromatography

    [0114] The crude enzyme solution was loaded into a column containing Q-resin, and then the eluted solution was collected. The column was washed with 1 ml of 20 mM Tris-HCl (pH 8.0) buffer, and elution solutions containing 100 mM NaCl, 200 mM NaCl, 300 mM NaCl, 400 mM NaCl and 500 mM NaCl 1M, respectively, in buffer, were sequentially loaded into the column in an amount of 250 μl for each elution solution, and the eluate was collected for each zone.

    [0115] (2) Cation Exchange Resin Chromatography

    [0116] Using a column containing SP-resin, each target protein was separated/purified in the same manner as (1) anion exchange resin chromatography.

    [0117] Finally, six desired target proteins were separated/purified through the above ion exchange chromatography method (FIGS. 5A, 5B, 5C, 5D, 5E and 5F). Finally, each high-purity protein was separated/purified from the cell lysate using the same method as described above.

    Example 5. Analysis of Expression and Size of Each Target Protein

    [0118] The proteins whose expression and size were confirmed on the SDS-PAGE gel were identified using the in-gel digestion method and the nano-LC-MS/MS method, thereby confirming the type of antibiotic resistance protein actually expressed in the strain (FIGS. 7A, 7B, 7C, 7D, 7E, 7F, 7G, 7H, 7I, 7J, 7K, 7L, 7M, 7N, 7O, 7P, 7Q, 7R, 7S and 7T).

    [0119] (1) In-Gel Digestion

    [0120] Only the band portion corresponding to the size of each target protein on the SDS-PAGE gel was obtained, and the stained gel was destained. The destained gel was subjected to a reduction/alkylation process, and then the protein was selectively digested using a trypsin enzyme. The digested peptides were recovered and desalted using DK-Tip (C18 Tip).

    [0121] (2) Nano-LC-MS/MS

    [0122] In order to confirm the sequence and coverage of the active protein expressed in the strain, nano liquid chromatography and high-resolution mass spectrometry were performed (Q-Exactive HF-X mass spectrometry system). The desalted peptide sample was dissolved with 0.1% formic acid solution and then loaded into a column. The peptide sample was separated using a C18 column (75 μm×70 cm) and nanoflow liquid chromatography. Examples of the gradient conditions for sample loading and separation used in this case are as follows: [0123] buffer A: 0.1% formic acid in water/buffer B: 0.1% formic acid in acetonitrile [0124] sample loading: from 0 to 5 min, 5% (B), 5 μL/min flow rate [0125] concentration gradient for separation:

    [0126] from 5 to 7 min, from 5% to 10% (B), 300 nL/min flow rate

    [0127] from 7 to 38 min, from 10% to 40% (B), 300 nL/min flow rate

    [0128] from 38 to 38.5 min, from 40% to 80% (B), 300 nL/min flow rate

    [0129] from 38.5 to 39.5 min, 80% (B), 300 nL/min flow rate

    [0130] from 39.5 to 40 min, from 80% to 5% (B), 300 nL/min flow rate

    [0131] from 40 to 60 min, 5% (B), 300 nL/min flow rate

    [0132] Examples of the parameters of the mass spectrometer used in this case are as follows: [0133] resolution: Full MS 60,000, MS2 30,000 [0134] Full MS: 350 to 2,000 m/z, 100 msec [0135] MS2: 50 msec, NCE 28, 1, ionized materials with a charge state of >6 were excluded from MS2 analysis

    [0136] ‘Proteome Discoverer (v2.4)’ software (Thermo scientific) was used to identify peptides and proteins based on bottom-up data, and protein/peptide identification was based on a FDR (false discovery rate) of 1%. Among proteins from E. coli, KPC, OXA, MBL proteins or GES protein was identified, 200 peptides (Table 2) for KPC protein, 177 peptides (Table 3) for OXA, 109 peptides (Table 4) for NDM protein, 146 peptides (Table 5) for IMP (Table 5), 159 peptides (Table 6) for VIM protein, and 111 peptides (Table 7) for GES were identified.

    TABLE-US-00003 TABLE 2 Information on position and sequence of each peptide identified as KPC protein Oxidation SEQUEST SEQUEST Observed Actual Start Stop Sequence site z XCorr deltaCn m/z mass (Da) 22 35 (S)ATALTNLVAEPFAK(L) 2 4.48 0.67 723.40 1,444.79 23 35 (A)TALTNLVAEPFAK(L) 2 4.56 0.67 687.88 1,373.75 24 35 (T)ALTNLVAEPFAK(L) 2 3.17 0.53 637.36 1,272.71 25 35 (A)LTNLVAEPFAK(L) 2 3.38 0.56 601.84 1,201.67 26 36 (L)TNLVAEPFAKL(E) 2 2.05 0.27 601.84 1,201.67 26 60 (L)TNLVAEPFAKLEQDFGGSIGVY M(49) 3 2.99 0.50 1,223.26 3,666.76 AmDTGSGATVSYR(A) 27 35 (T)NLVAEPFAK(L) 2 2.25 0.33 494.78 987.54 29 35 (L)VAEPFAK(L) 1 2.65 0.43 761.41 760.41 34 47 (F)AKLEQDFGGSIGVY(A) 2 3.15 0.52 742.38 1,482.74 34 60 (F)AKLEQDFGGSIGVYAmDTGSGA M(49) 3 5.39 0.72 932.78 2,795.31 TVSYR(A) 34 60 (F)AKLEQDFGGSIGVYAMDTGSGA 3 3.97 0.62 927.45 2,779.32 TVSYR(A) 36 60 (K)LEQDFGGSIGVYAmDTGSGATV M(49) 2 6.81 0.78 1,299.10 2,596.18 SYR(A) 36 60 (K)LEQDFGGSIGVYAMDTGSGATV 2 6.83 0.78 1,291.10 2,580.18 SYR(A) 42 60 (G)GSIGVYAmDTGSGATVSYR(A) M(49) 2 2.01 0.25 954.44 1,906.87 45 60 (I)GVYAMDTGSGATVSYR(A) 2 2.35 0.36 817.88 1,633.74 48 59 (Y)AmDTGSGATVSY(R) M(49) 2 2.84 0.47 588.25 1,174.48 48 59 (Y)AMDTGSGATVSY(R) 2 2.67 0.44 580.25 1,158.49 48 60 (Y)AmDTGSGATVSYR(A) M(49) 2 3.30 0.55 666.30 1,330.58 48 60 (Y)AMDTGSGATVSYR(A) 2 3.92 0.62 658.30 1,314.59 50 60 (M)DTGSGATVSYR(A) 2 2.25 0.33 557.27 1,112.52 60 67 (Y)RAEERFPL(C) 2 1.76 0.15 509.28 1,016.54 60 71 (Y)RAEERFPLcSSF(K) 2 2.80 0.46 749.86 1,497.71 61 67 (R)AEERFPL(C) 2 1.98 0.24 431.23 860.44 61 72 (R)AEERFPLcSSFK(G) 2 4.26 0.65 735.86 1,469.70 62 72 (A)EERFPLcSSFK(G) 2 3.39 0.56 700.34 1,398.67 63 72 (E)ERFPLcSSFK(G) 2 3.86 0.61 635.82 1,269.62 65 72 (R)FPLcSSFK(G) 2 2.35 0.36 493.25 984.48 68 74 (L)cSSFKGF(L) 2 2.16 0.31 416.69 831.36 72 80 (F)KGFLAAAVL(A) 72 82 (F)KGFLAAAVLAR(S) 2 2.01 0.25 558.85 1,115.69 73 82 (K)GFLAAAVLAR(S) 2 3.21 0.53 494.80 987.59 74 82 (G)FLAAAVLAR(S) 2 1.97 0.24 466.29 930.56 75 82 (F)LAAAVLAR(S) 2 2.96 0.49 392.76 783.50 76 82 (L)AAAVLAR(S) 1 2.24 0.33 671.42 670.41 81 96 (L)ARSQQQAGLLDTPIRY(G) 2 3.60 0.58 908.99 1,815.96 83 95 (R)SQQQAGLLDTPIR(Y) 2 4.74 0.68 713.88 1,425.75 83 98 (R)SQQQAGLLDTPIRYGK(N) 2 5.57 0.73 887.98 1,773.94 84 95 (S)QQQAGLLDTPIR(Y) 2 2.45 0.39 670.37 1,338.73 85 95 (Q)QQAGLLDTPIR(Y) 2 3.39 0.56 606.34 1,210.67 87 95 (Q)AGLLDTPIR(Y) 2 2.37 0.37 478.28 954.55 90 96 (L)LDTPIRY(G) 2 1.96 0.23 439.24 876.47 96 110 (R)YGKNALVPWSPISEK(Y) 2 1.87 0.20 844.95 1,687.89 97 104 (Y)GKNALVPW(S) 2 1.89 0.21 442.75 883.50 97 111 (Y)GKNALVPWSPISEKY(L) 2 4.27 0.65 844.96 1,687.90 99 110 (K)NALVPWSPISEK(Y) 2 3.22 0.53 670.86 1,339.71 100 110 (N)ALVPWSPISEK(Y) 2 2.11 0.29 613.84 1,225.67 102 110 (L)VPWSPISEK(Y) 1 2.69 0.44 1,042.55 1,041.54 103 110 (V)PWSPISEK(Y) 2 2.19 0.31 472.25 942.48 105 111 (W)SPISEKY(L) 2 1.90 0.21 412.22 822.42 105 112 (W)SPISEKYL(T) 2 1.87 0.20 468.76 935.50 105 121 (W)SPISEKYLTTGmTVAEL(S) M(116) 2 3.60 0.58 928.47 1,854.93 111 139 (K)YLTTGmTVAELSAAAVQYSDN M(116) 2 8.51 0.82 1,508.27 3,014.53 AAANLLLK(E) 111 139 (K)YLTTGMTVAELSAAAVQYSDN 2 8.88 0.83 1,500.27 2,998.53 AAANLLLK(E) 112 121 (Y)LTTGmTVAEL(S) M(116) 2 1.90 0.21 526.27 1,050.53 113 139 (L)TTGMTVAELSAAAVQYSDNAA 2 3.10 0.52 1,362.20 2,722.39 ANLLLK(E) 117 139 (M)TVAELSAAAVQYSDNAAANLL 2 5.53 0.73 1,167.12 2,332.23 LK(E) 122 128 (L)SAAAVQY(S) 1 1.62 0.07 709.35 708.35 122 139 (L)SAAAVQYSDNAAANLLLK(E) 2 4.23 0.65 910.48 1,818.94 123 139 (S)AAAVQYSDNAAANLLLK(E) 2 3.20 0.53 866.97 1,731.92 125 139 (A)AVQYSDNAAANLLLK(E) 2 3.41 0.56 795.93 1,589.84 128 139 (Q)YSDNAAANLLLK(E) 2 3.76 0.60 646.85 1,291.68 129 137 (Y)SDNAAANLL(L) 1 2.19 0.32 888.45 887.44 129 139 (Y)SDNAAANLLLK(E) 2 2.65 0.43 565.31 1,128.61 138 147 (L)LKELGGPAGL(T) 2 3.57 0.58 477.79 953.56 139 150 (L)KELGGPAGLTAF(M) 2 2.89 0.48 580.82 1,159.63 140 152 (K)ELGGPAGLTAFmR(S) M(151) 2 3.56 0.58 668.34 1,334.67 140 152 (K)ELGGPAGLTAFMR(S) 2 3.94 0.62 660.34 1,318.67 142 152 (L)GGPAGLTAFmR(S) M(151) 2 2.11 0.29 547.28 1,092.54 143 152 (G)GPAGLTAFmR(S) M(151) 2 2.63 0.43 518.77 1,035.52 144 152 (G)PAGLTAFmR(S) M(151) 2 2.42 0.38 490.25 978.49 148 159 (L)TAFmRSIGDTTF(R) M(151) 2 3.43 0.56 681.82 1,361.63 148 159 (L)TAFMRSIGDTTF(R) 2 3.89 0.61 673.83 1,345.64 151 159 (F)mRSIGDTTF(R) M(151) 2 2.57 0.42 522.25 1,042.48 151 159 (F)MRSIGDTTF(R) 2 2.52 0.40 514.25 1,026.49 153 160 (R)SIGDTTFR(L) 2 2.15 0.30 448.73 895.44 153 163 (R) SIGDTTFRLDR(W) 2 2.55 0.41 640.83 1,279.65 153 177 (R)SIGDTTFRLDRWELELNSAIPGD 3 1.99 0.25 944.82 2,831.43 AR(D) 154 160 (S)IGDTTFR(L) 2 2.08 0.28 405.21 808.41 154 163 (S)IGDTTFRLDR(W) 2 1.88 0.20 597.32 1,192.62 155 163 (I)GDTTFRLDR(W) 2 1.85 0.19 540.78 1,079.54 156 163 (G)DTTFRLDR(W) 2 1.92 0.22 512.27 1,022.52 161 177 (R)LDRWELELNSAIPGDAR(D) 2 3.95 0.62 978.00 1,953.99 161 183 (R)LDRWELELNSAIPGDARDTSSPR 3 2.59 0.42 866.77 2,597.29 (A) 164 177 (R)WELELNSAIPGDAR(D) 2 4.22 0.64 785.90 1,569.78 164 183 (R)WELELNSAIPGDARDTSSPR(A) 3 3.34 0.55 738.70 2,213.08 165 177 (W)ELELNSAIPGDAR(D) 2 3.42 0.56 692.86 1,383.70 184 191 (R)AVTESLQK(L) 2 2.27 0.34 438.24 874.47 184 203 (R)AVTESLQKLTLGSALAAPQR(Q) 2 3.16 0.53 1,027.58 2,053.15 185 191 (A)VTESLQK(L) 2 1.97 0.24 402.73 803.44 192 203 (K)LTLGSALAAPQR(Q) 2 4.07 0.63 599.35 1,196.69 192 204 (K)LTLGSALAAPQRQ(Q) 2 1.85 0.19 663.38 1,324.75 193 203 (L)TLGSALAAPQR(Q) 2 2.24 0.33 542.81 1,083.61 193 206 (L)TLGSALAAPQRQQF(V) 2 4.04 0.63 744.40 1,486.79 194 203 (T)LGSALAAPQR(Q) 2 3.44 0.56 492.28 982.56 195 206 (L)GSALAAPQRQQF(V) 2 2.10 0.29 637.34 1,272.66 199 209 (L)AAPQRQQFVDW(L) 2 3.25 0.54 673.34 1,344.66 199 210 (L)AAPQRQQFVDWL(K) 2 1.82 0.18 729.88 1,457.74 204 211 (R)QQFVDWLK(G) 2 2.89 0.48 532.28 1,062.55 204 219 (R)QQFVDWLKGNTTGNHR(I) 2 2.91 0.48 950.98 1,899.94 210 228 (W)LKGNTTGNHRIRAAVPADW(A) 3 1.93 0.22 693.04 2,076.11 212 219 (K)GNTTGNHR(I) 2 2.39 0.37 428.70 855.39 222 233 (R)AAVPADWAVGDK(T) 2 2.66 0.44 600.31 1,198.60 222 235 (R)AAVPADWAVGDKTG(T) 2 3.29 0.54 679.34 1,356.67 222 236 (R)AAVPADWAVGDKTGT(C) 2 3.82 0.61 729.86 1,457.71 222 254 (R)AAVPADWAVGDKTGTcGVYGT 3 3.57 0.58 1,142.54 3,424.60 ANDYAVVWPTGR(A) 223 233 (A)AVPADWAVGDK(T) 2 2.43 0.38 564.79 1,127.56 224 233 (A)VPADWAVGDK(T) 2 2.89 0.48 529.27 1,056.52 225 233 (V)PADWAVGDK(T) 2 3.15 0.52 479.73 957.46 226 233 (P)ADWAVGDK(T) 1 2.59 0.42 861.41 860.40 229 240 (W)AVGDKTGTcGVY(G) 2 3.20 0.53 614.29 1,226.56 229 246 (W)AVGDKTGTcGVYGTANDY(A) 2 3.02 0.50 924.91 1,847.81 234 254 (K)TGTcGVYGTANDYAVVWPTGR 2 3.04 0.51 1,123.02 2,244.02 (A) 238 254 (C)GVYGTANDYAVVWPTGR(A) 2 4.23 0.65 913.45 1,824.88 239 254 (G)VYGTANDYAVVWPTGR(A) 2 4.11 0.64 884.94 1,767.86 240 254 (V)YGTANDYAVVWPTGR(A) 2 3.37 0.55 835.40 1,668.79 241 254 (Y)GTANDYAVVWPTGR(A) 2 1.98 0.24 753.87 1,505.73 245 254 (N)DYAVVWPTGR(A) 2 2.69 0.44 582.29 1,162.58 247 259 (Y)AVVWPTGRAPIVL(A) 2 2.21 0.32 689.92 1,377.82 251 262 (W)PTGRAPIVL AVY(T) 2 2.40 0.38 628.87 1,255.73 255 264 (R)APIVLAVYTR(A) 2 3.49 0.57 551.83 1,101.65 255 271 (R)APIVLAVYTRAPNKDDK(H) 3 1.75 0.14 624.35 1,870.03 256 264 (A)PIVLAVYTR(A) 2 2.99 0.50 516.32 1,030.62 257 264 (P)IVLAVYTR(A) 2 1.99 0.25 467.79 933.56 260 283 (L)AVYTRAPNKDDKHSEAVIAAA 4 3.51 0.57 642.60 2,566.37 ARL(A) 263 283 (Y)TRAPNKDDKHSEAVIAAAARL(A) 3 5.25 0.71 745.41 2,233.20 263 285 (Y)TRAPNKDDKHSEAVIAAAARLA 3 3.67 0.59 806.78 2,417.33 L(E) 265 282 (R)APNKDDKHSEAVIAAAAR(L) 2 5.92 0.75 932.49 1,862.96 266 282 (A)PNKDDKHSEAVIAAAAR(L) 3 5.88 0.74 598.31 1,791.92 267 282 (P)NKDDKHSEAVIAAAAR(L) 2 4.96 0.70 848.44 1,694.87 268 282 (N)KDDKHSEAVIAAAAR(L) 2 5.04 0.70 791.42 1,580.82 269 282 (K)DDKHSEAVIAAAAR(L) 2 3.34 0.55 727.37 1,452.73 270 282 (D)DKHSEAVIAAAAR(L) 3 2.12 0.29 446.91 1,337.71 271 282 (D)KHSEAVIAAAAR(L) 2 3.35 0.55 612.35 1,222.68 272 282 (K)HSEAVIAAAAR(L) 2 3.92 0.62 548.30 1,094.58 273 282 (H)SEAVIAAAAR(L) 2 2.34 0.36 479.77 957.52 283 291 (R)LALEGLGVN(G) 1 2.36 0.36 885.50 884.49 283 293 (R)LALEGLGVNGQ(—) 2 3.66 0.59 535.80 1,069.58 284 293 (L)ALEGLGVNGQ(—) 2 2.09 0.28 479.25 956.49

    TABLE-US-00004 TABLE 3 Information on position and sequence of each peptide identified as OXA protein Actual Oxidation SEQUEST SEQUEST Observed mass Start Stop Sequence site z XCorr deltaCn m/z (Da) 23 29 (A)KEWQENK(S) 2 2.01 0.25 481.24 960.47 23 39 (A)KEWQENKSWNAHFTEHK(S) 4 5.28 0.72 550.52 2198.03 28 39 (E)NKSWNAHFTEHK(S) 2 3.06 0.51 749.87 1497.72 30 37 (K)SWNAHFTE(H) 2 1.78 0.16 496.22 990.43 30 38 (K)SWNAHFTEH(K) 2 3.14 0.52 564.75 1127.48 30 39 (K)SWNAHFTEHK(S) 2 3.71 0.60 628.80 1255.58 31 39 (S)WNAHFTEHK(S) 2 3.47 0.57 585.28 1168.54 32 39 (W)NAHFTEHK(S) 2 3.09 0.51 492.24 982.46 32 39 (W)NAHFTEHK(S) 3 2.80 0.46 328.50 982.47 33 39 (N)AHFTEHK(S) 2 2.06 0.27 435.22 868.42 38 51 (E)HKSQGVVVLWNENK(Q) 2 5.32 0.72 819.45 1636.88 40 49 (K) SQGVVVLWNE(N) 2 2.56 0.41 565.80 1129.58 40 51 (K)SQGVVVLWNENK(Q) 3 3.90 0.62 458.25 1371.71 40 60 (K)SQGVVVLWNENKQQGFTNNLK(R) 2 4.90 0.69 1202.14 2402.26 40 61 (K)SQGVVVLWNENKQQGFTNNLKR(A) 3 6.25 0.76 853.78 2558.33 42 51 (Q)GVVVLWNENK(Q) 2 3.13 0.52 579.32 1156.63 49 60 (N)ENKQQGFTNNLK(R) 2 2.79 0.46 710.87 1419.72 49 61 (N)ENKQQGFTNNLKR(A) 3 4.16 0.64 526.28 1575.82 50 60 (E)NKQQGFTNNLK(R) 2 3.50 0.57 646.34 1290.67 50 61 (E)NKQQGFTNNLKR(A) 3 5.40 0.72 483.27 1446.77 51 60 (N)KQQGFTNNLK(R) 2 3.73 0.60 589.32 1176.63 51 61 (N)KQQGFTNNLKR(A) 2 2.07 0.28 667.37 1332.73 52 60 (K)QQGFTNNLK(R) 2 3.28 0.54 525.27 1048.53 52 61 (K)QQGFTNNLKR(A) 3 3.92 0.62 402.55 1204.64 53 61 (Q)QGFTNNLKR(A) 2 2.93 0.49 539.29 1076.57 54 60 (Q)GFTNNLK(R) 1 2.16 0.31 793.42 792.41 54 61 (Q)GFTNNLKR(A) 2 2.44 0.38 475.26 948.51 55 61 (G)FTNNLKR(A) 2 2.40 0.37 446.75 891.50 61 72 (K)RANQAFLPASTF(K) 2 2.99 0.50 661.85 1321.68 61 73 (K)RANQAFLPASTFK(I) 2 4.14 0.64 725.90 1449.78 62 72 (R)ANQAFLPASTF(K) 2 2.30 0.35 583.80 1165.58 62 73 (R)ANQAFLPASTFK(I) 2 3.95 0.62 647.85 1293.68 62 76 (R)ANQAFLPASTFKIPN(S) 2 2.10 0.29 809.94 1617.86 63 73 (A)NQAFLPASTFK(I) 2 3.07 0.51 612.33 1222.64 64 73 (N)QAFLPASTFK(I) 2 3.31 0.55 555.31 1108.60 65 73 (Q)AFLPASTFK(I) 2 1.95 0.23 491.28 980.54 66 73 (A)FLPASTFK(I) 1 2.53 0.41 910.51 909.50 67 73 (F)LPASTFK(I) 1 3.04 0.51 763.43 762.43 74 87 (K)IPNSLIALDLGVVK(D) 2 3.39 0.56 726.45 1450.88 74 94 (K)IPNSLIALDLGVVKDEHQVFK(W) 3 8.46 0.82 779.11 2334.30 74 100 (K)IPNSLIALDLGVVKDEHQVFKWDGQ 5 2.45 0.39 616.53 3077.63 TR(D) 75 94 (I)PNSLIALDLGWKDEHQVFK(W) 4 5.10 0.71 556.31 2221.20 77 94 (N)SLIALDLGVVKDEHQVFK(W) 2 4.75 0.68 1006.07 2010.12 80 94 (I)ALDLGWKDEHQVFK(W) 2 2.13 0.30 849.46 1696.91 81 94 (A)LDLGVVKDEHQVFK(W) 2 1.82 0.18 813.95 1625.88 82 94 (L)DLGVVKDEHQVFK(W) 2 3.04 0.51 757.40 1512.79 83 94 (D)LGVVKDEHQVFK(W) 2 3.16 0.53 699.89 1397.77 83 100 (D)LGVVKDEHQVFKWDGQTR(D) 3 3.36 0.55 714.71 2141.11 84 94 (L)GVVKDEHQVFK(W) 2 3.26 0.54 643.35 1284.68 88 95 (K)DEHQVFKW(D) 2 2.60 0.42 544.76 1087.51 88 100 (K)DEHQVFKWDGQTR(D) 2 3.91 0.62 823.39 1644.77 89 100 (D)EHQVFKWDGQTR(D) 3 1.82 0.18 510.92 1529.74 90 100 (E)HQVFKWDGQTR(D) 3 3.49 0.57 467.91 1400.70 92 100 (Q)VFKWDGQTR(D) 2 1.92 0.22 568.80 1135.58 96 107 (W)DGQTRDIATWNR(D) 3 3.29 0.54 478.24 1431.69 101 107 (R)DIATWNR(D) 2 2.13 0.30 438.22 874.43 101 110 (R)DIATWNRDHN(L) 2 1.94 0.23 621.29 1240.56 101 116 (R)DIATWNRDHNLITAmK(Y) M(115) 3 5.46 0.73 638.99 1913.94 101 116 (R)DIATWNRDHNLITAMK(Y) 3 5.23 0.71 633.66 1897.95 103 116 (I)ATWNRDHNLITAmK(Y) M(115) 3 6.00 0.75 562.95 1685.83 103 116 (I)ATWNRDHNLITAMK(Y) 3 3.52 0.57 557.62 1669.84 104 116 (A)TWNRDHNLITAmK(Y) M(115) 3 2.18 0.31 539.27 1614.79 105 128 (T)WNRDHNLITAMKYSVVPVYQEFAR 3 2.26 0.34 979.83 2936.46 (Q) 107 116 (N)RDHNLITAMK(Y) 2 2.89 0.48 599.82 1197.63 108 116 (R)DHNLITAmK(Y) M(115) 2 3.80 0.61 529.77 1057.53 108 116 (R)DHNLITAMK(Y) 2 3.45 0.56 521.77 1041.53 108 128 (R)DHNLITAmKYSVVPVYQEFAR(Q) M(115) 4 2.38 0.37 625.07 2496.25 109 116 (D)HNLITAMK(Y) 2 3.74 0.60 464.26 926.50 116 128 (M)KYSVVPVYQEFAR(Q) 2 3.73 0.60 793.43 1584.84 117 125 (K)YSVVPVYQE(F) 2 1.93 0.22 542.27 1082.53 117 126 (K)YSVVPVYQEF(A) 2 2.25 0.33 615.81 1229.60 117 128 (K)YSVVPVYQEFAR(Q) 2 3.11 0.52 729.38 1456.74 121 128 (V)PVYQEFAR(Q) 2 1.86 0.19 505.26 1008.51 138 152 (K)mLHAFDYGNEDISGN(V) M(138) 2 2.27 0.34 849.86 1697.71 138 155 (K)MLHAFDYGNEDISGNVDS(F) 2 2.38 0.37 992.43 1982.84 138 163 (K)mLHAFDYGNEDISGNVDSFWLDGGI M(138) 3 3.08 0.51 982.11 2943.31 R(I) 138 163 (K)MLHAFDYGNEDISGNVDSFWLDGGI 2 3.14 0.52 1464.67 2927.32 R(I) 143 153 (F)DYGNEDISGNV(D) 2 2.50 0.40 591.75 1181.49 147 163 (N)EDISGNVDSFWLDGGIR(I) 2 2.56 0.41 940.44 1878.87 153 163 (N)VDSFWLDGGIR(I) 2 2.90 0.48 632.82 1263.63 164 174 (R)ISATEQISFLR(K) 2 4.43 0.66 632.85 1263.68 164 175 (R)ISATEQISFLRK(L) 2 4.21 0.64 696.90 1391.79 165 174 (I)SATEQISFLR(K) 2 2.42 0.38 576.31 1150.60 165 175 (I)SATEQISFLRK(L) 2 3.50 0.57 640.36 1278.70 166 174 (S)ATEQISFLR(K) 2 2.46 0.39 532.79 1063.56 166 175 (S)ATEQISFLRK(L) 2 3.28 0.54 596.84 1191.67 167 174 (A)TEQISFLR(K) 2 2.28 0.34 497.27 992.53 167 175 (A)TEQISFLRK(L) 2 2.74 0.45 561.32 1120.63 169 175 (E)QISFLRK(L) 3 2.05 0.27 297.85 890.54 175 186 (R)KLYHNKLHVSER(S) 3 2.58 0.42 508.62 1522.84 176 186 (K)LYHNKLHVSER(S) 3 2.90 0.48 465.92 1394.75 190 206 (R)IVKQAmLTEANGDYIIR(A) M(195) 2 3.21 0.53 976.03 1950.04 193 200 (K)QAMLTEAN(G) 1 2.61 0.42 877.41 876.40 193 206 (K)QAmLTEANGDYIIR(A) M(195) 2 3.19 0.53 805.89 1609.77 193 206 (K)QAMLTEANGDYIIR(A) 2 4.43 0.66 797.90 1593.79 194 206 (Q)AmLTEANGDYIIR(A) M(195) 2 3.76 0.60 741.87 1481.73 196 206 (M)LTEANGDYIIR(A) 2 2.90 0.48 632.83 1263.65 207 218 (R)AKTGYSTRIEPK(I) 3 2.31 0.35 450.92 1349.74 209 218 (K)TGYSTRIEPK(I) 2 2.21 0.32 576.31 1150.60 210 218 (T)GYSTRIEPK(I) 2 1.81 0.17 525.79 1049.56 219 250 (K)IGWWVGWVELDDNVWFFAmNmDm M(237, 4 1.86 0.19 952.18 3804.69 PTSDGLGLR(Q) 239, 241) 235 250 (F)FAMNMDMPTSDGLGLR(Q) 2 3.17 0.53 878.40 1754.78 236 250 (F)AMNmDmPTSDGLGLR(Q) M(239, 2 3.15 0.52 820.86 1639.71 241) 236 250 (F)AMNMDMPTSDGLGLR(Q) 2 2.98 0.50 804.86 1607.71 238 250 (M)NmDMPTSDGLGLR(Q) M(239) 2 3.11 0.52 711.82 1421.63 238 250 (M)NMDMPTSDGLGLR(Q) 2 2.95 0.49 703.82 1405.63 239 250 (N)mDmPTSDGLGLR(Q) M(239, 2 2.39 0.37 662.80 1323.58 241) 245 265 (S)DGLGLRQAITKEVLKQEKIIP(—) 3 2.55 0.41 783.80 2348.39 251 262 (R)QAITKEVLKQEK(I) 2 4.24 0.65 707.92 1413.82 256 262 (K)EVLKQEK(I) 2 2.55 0.41 437.26 872.50 256 265 (K)EVLKQEKIIP(—) 2 2.11 0.29 598.87 1195.72

    TABLE-US-00005 TABLE 4 Information on position and sequence of each peptide identified as NDM protein Actual SEQUEST mass Oxidation Charge SEQUEST delta Observed value Start Stop Sequence site (z) XCorr Cn m/z (Da) 28 45 (M)PGEIRPTIGQQmETGDQR(F) M(39) 3 3.61 0.58 677.00 2027.98 28 45 (M)PGEIRPTIGQQMETGDQR(F) 3 2.30 0.35 671.67 2011.99 30 45 (G)EIRPTIGQQMETGDQR(F) 3 1.64 0.09 620.31 1857.91 33 45 (R)PTIGQQmETGDQR(F) M(39) 2 2.33 0.36 738.84 1475.67 33 45 (R)PTIGQQMETGDQR(F) 2 2.31 0.35 730.84 1459.68 35 45 (T)IGQQmETGDQR(F) M(39) 2 2.27 0.34 639.79 1277.57 45 52 (Q)RFGDLVFR(Q) 2 1.39 0.00 505.29 1008.56 46 52 (R)FGDLVFR(Q) 2 2.72 0.45 427.23 852.45 47 52 (F)GDLVFR(Q) 1 1.19 0.00 706.39 705.38 53 61 (R)QLAPNVWQH(T) 2 1.33 0.00 546.79 1091.56 53 76 (R)QLAPNVWQHTSYLDMPGFGAVASN 2 1.97 0.24 1302.13 2602.25 (G) 53 81 (R)QLAPNVWQHTSYLDmPGFGAVASN M(67) 3 3.91 0.62 1053.20 3156.58 GLIVR(D) 53 81 (R)QLAPNVWQHTSYLDMPGFGAVASN 3 5.86 0.74 1047.87 3140.60 GLIVR(D) 53 82 (R)QLAPNVWQHTSYLDMPGFGAVASN 4 1.61 0.07 814.92 3255.64 GLIVRD(G) 53 85 (R)QLAPNVWQHTSYLDmPGFGAVASN M(67) 4 2.41 0.38 886.45 3541.77 GLIVRDGGR(V) 58 81 (N)VWQHTSYLDMPGFGAVASNGLIVR 3 2.26 0.34 873.45 2617.34 (D) 62 81 (H)TSYLDmPGFGAVASNGLIVR(D) M(67) 2 3.46 0.57 1042.53 2083.05 68 81 (M)PGFGAVASNGLIVR(D) 2 2.92 0.49 679.39 1356.76 71 81 (F)GAVASNGLIVR(D) 2 1.41 0.00 528.82 1055.62 86 92 (R)VLVVDTA(W) 1 0.79 0.00 716.42 715.41 86 102 (R)VLVVDTAWTDDQTAQIL(N) 2 2.06 0.27 944.49 1886.97 86 106 (R)VLVVDTAWTDDQTAQILNWIK(Q) 2 4.86 0.69 1215.15 2428.28 91 106 (D)TAWTDDQTAQILNWIK(Q) 2 1.98 0.24 952.49 1902.96 94 106 (W)TDDQTAQILNWIK(Q) 2 2.84 0.47 773.40 1544.79 96 106 (D)DQTAQILNWIK(Q) 2 1.71 0.12 665.37 1328.72 104 125 (N)WIKQEINLPVALAVVTHAHQDK(M) 3 1.84 0.19 837.47 2509.40 107 120 (K)QEINLPVALAVVTH(A) 2 2.24 0.33 752.43 1502.85 107 121 (K)QEINLPVALAVVTHA(H) 2 2.26 0.34 787.95 1573.89 107 124 (K)QEINLPVALAVVTHAHQD(K) 3 3.06 0.51 652.35 1954.03 107 125 (K)QEINLPVALAVVTHAHQDK(M) 3 7.39 0.80 695.05 2082.13 110 125 (I)NLPVALAVVTHAHQDK(M) 3 2.55 0.41 571.66 1711.95 ill 125 (N)LPVALAVVTHAHQDK(M) 2 2.58 0.42 799.96 1597.90 112 125 (LJPVALAVVTHAHQDK(M) 3 5.46 0.73 495.95 1484.82 114 125 (V)ALAVVTHAHQDK(M) 2 3.84 0.61 645.35 1288.69 115 125 (A)LAVVTHAHQDK(M) 2 3.89 0.61 609.84 1217.66 116 125 (L)AVVTHAHQDK(M) 2 3.30 0.55 553.29 1104.57 117 125 (A)VVTHAHQDK(M) 2 2.14 0.30 517.78 1033.54 118 125 (V)VTHAHQDK(M) 1 1.59 0.06 935.47 934.46 119 125 (V)THAHQDK(M) 1 0.55 0.03 836.40 835.39 126 135 (K)mGGmDALHAA(G) M(126), 2 1.65 0.09 503.21 1004.41 M(129) 126 140 (K)mGGmDALHAAGIATY(A) M(126), 2 1.40 0.00 755.84 1509.67 M(129) 126 140 (K)MGGMDALHAAGIATY(A) 2 1.63 0.08 739.85 1477.68 126 142 (K)MGGmDALHAAGIATYAN(A) M(129) 2 1.93 0.00 840.38 1678.75 126 142 (K)mGGmDALHAAGIATYAN(A) M(126), 2 2.78 0.46 848.38 1694.75 M(129) 126 142 (K)MGGMDALHAAGIATYAN(A) 2 2.25 0.33 832.39 1662.76 126 144 (K)MGGMDALHAAGIATYANAL(S) 2 2.24 0.33 924.45 1846.88 126 146 (K)mGGMDALHAAGIATYANALSN(Q) M(126) 2 2.18 0.04 1032.98 2063.95 126 146 (K)MGGMDALHAAGIATYANALSN(Q) 2 2.10 0.29 1024.99 2047.96 126 159 (K)mGGmDALHAAGIATYANALSNQLA M(126), 3 2.59 0.42 1147.88 3440.61 PQEGMVAAQH(S) M(129) 126 159 (K)MGGMDALHAAGIATYANALSNQLA 3 3.73 0.60 1137.22 3408.63 PQEGMVAAQH(S) 126 161 (K)MGGMDALHAAGIATYANALSNQLA 3 3.83 0.61 1203.92 3608.74 PQEGMVAAQHSL(T) 126 163 (K)MGGMDALHAAGIATYANALSNQLA 3 3.62 0.59 1286.63 3856.85 PQEGMVAAQHSLTF(A) 126 166 (K)MGGMDALHAAGIATYANALSNQLA 3 2.81 0.47 1372.00 4112.98 PQEGMVAAQHSLTFAAN(G) 143 181 (N)ALSNQLAPQEGmVAAQHSLTFAANG M(153) 4 2.20 0.32 1013.25 4048.97 WVEPATAPNFGPLK(V) 143 181 (N)ALSNQLAPQEGMVAAQHSLTFAAN 3 4.58 0.67 1345.36 4033.05 GWVEPATAPNFGPLK(V) 167 181 (N)GWVEPATAPNFGPLK(V) 2 1.99 0.25 792.41 1582.81 168 181 (G)WVEPATAPNFGPLK(V) 2 2.36 0.36 763.91 1525.80 169 181 (W)VEPATAPNFGPLK(V) 2 2.04 0.26 670.87 1339.72 171 181 (E)PATAPNFGPLK(V) 2 2.24 0.33 556.81 1111.61 173 181 (A)TAPNFGPLK(V) 2 1.59 0.05 472.77 943.52 175 181 (A)PNFGPLK(V) 2 2.07 0.28 386.72 771.43 182 202 (K)VFYPGPGHTSDNITVGIDGTD(I) 2 2.26 0.34 1081.51 2161.01 182 207 (K)VFYPGPGHTSDNITVGIDGTDIAFGG 2 1.78 0.16 1304.13 2606.24 (C) 182 208 (K)VFYPGPGHTSDNITVGIDGTDIAFGGc 2 2.85 0.47 1384.14 2766.26 C) 182 211 (K)VFYPGPGHTSDNITVGIDGTDIAFGGc 3 6.14 0.76 1041.19 3120.54 LIK(D) 182 214 (K)VFYPGPGHTSDNITVGIDGTDIAFGGc 3 8.25 0.82 1151.24 3450.71 LIKDSK(A) 182 216 (K)VFYPGPGHTSDNITVGIDGTDIAFGGc 4 3.32 0.55 913.47 3649.84 LIKDSKAK(S) 185 216 (Y)PGPGHTSDNITVGIDGTDIAFGGcLIK 3 1.88 0.20 1081.20 3240.59 DSKAK(S) 190 214 (H)TSDNITVGIDGTDIAFGGcLIKDSK(A) 3 3.11 0.52 866.43 2596.28 193 211 (D)NITVGIDGTDIAFGGcLIK(D) 2 4.00 0.63 982.52 1963.03 194 211 (N)ITVGIDGTDIAFGGcLIK(D) 2 2.98 0.50 925.50 1848.98 194 214 (N)ITVGIDGTDIAFGGcLIKDSK(A) 3 1.93 0.22 727.38 2179.12 200 211 (D)GTDIAFGGcLIK(D) 2 1.56 0.04 626.33 1250.64 200 214 (D)GTDIAFGGcLIKDSK(A) 2 2.57 0.42 791.41 1580.80 203 214 (D)IAFGGcLIKDSK(A) 2 1.63 0.08 654.86 1307.70 215 234 (K)AKSLGNLGDADTEHYAASAR(A) 2 4.25 0.65 1024.00 2045.99 217 223 (K)SLGNLGD(A) 1 0.69 0.00 675.33 674.32 217 229 (K)SLGNLGDADTEHY(A) 2 1.87 0.20 696.31 1390.61 217 231 (K)SLGNLGDADTEHYAA(S) 2 1.75 0.14 767.35 1532.69 217 232 (K)SLGNLGDADTEHYAAS(A) 2 2.34 0.36 810.87 1619.72 217 234 (K)SLGNLGDADTEHYAASAR(A) 2 5.99 0.75 924.43 1846.85 217 242 (K)SLGNLGDADTEHYAASARAFGAAFP 3 4.83 0.69 879.77 2636.28 K(A) 218 234 (S)LGNLGDADTEHYAASAR(A) 3 2.68 0.44 587.61 1759.81 219 234 (L)GNLGDADTEHYAASAR(A) 2 4.78 0.69 824.37 1646.73 220 234 (G)NLGDADTEHYAASAR(A) 2 2.16 0.31 795.86 1589.70 221 234 (N)LGDADTEHYAASAR(A) 2 4.99 0.70 738.85 1475.68 222 234 (L)GDADTEHYAASAR(A) 2 4.57 0.67 682.30 1362.58 225 234 (A)DTEHYAASAR(A) 2 1.83 0.18 560.75 1119.49 235 242 (R)AFGAAFPK(A) 2 2.26 0.34 404.72 807.43 235 256 (R)AFGAAFPKASmIVMSHSAPDSR(A) M(245) 2 2.65 0.00 1147.56 2293.11 235 256 (R)AFGAAFPKASmlVmSHSAPDSR(A) M(245), 3 1.86 0.19 770.71 2309.11 M(248) 235 256 (R)AFGAAFPKASMIVMSHSAPDSR(A) 2 3.89 0.61 1139.56 2277.11 236 242 (A)FGAAFPK(A) 2 1.97 0.24 369.20 736.39 237 242 (F)GAAFPK(A) 1 1.22 0.00 590.33 589.32 243 254 (K)ASMIVMSHSAPD(S) 2 1.67 0.10 623.29 1244.56 243 256 (K)ASmIVMSHSAPDSR(A) M(245) 3 5.03 0.70 502.24 1503.69 243 256 (K)ASmIVmSHSAPDSR(A) M(245), 3 4.91 0.69 507.57 1519.69 M(248) 243 256 (K)ASMIVMSHSAPDSR(A) 3 5.74 0.74 496.91 1487.69 244 256 (A)SmIVmSHSAPDSR(A) M(248) 2 1.67 0.10 725.33 1448.64 244 256 (A)SMIVMSHSAPDSR(A) 2 4.54 0.67 709.33 1416.65 245 256 (S)MIVmSHSAPDSR(A) M(248) 2 2.85 0.47 673.82 1345.62 245 256 (S)MIVMSHSAPDSR(A) 2 3.54 0.58 665.82 1329.62 246 256 (M)IVmSHSAPDSR(A) M(248) 2 3.10 0.52 608.29 1214.57 247 256 (I)VMSHSAPDSR(A) 2 3.06 0.51 543.76 1085.50 251 256 (H)SAPDSR(A) 1 0.54 0.00 632.30 631.29 257 264 (R)AAITHTAR(M) 2 2.74 0.45 420.74 839.46 258 264 (A)AITHTAR(M) 2 1.81 0.17 385.22 768.42 259 264 (A)ITHTAR(M) 1 1.38 0.00 698.39 697.39 265 270 (R)mADKLR(—) M(265) 2 2.08 0.28 375.20 748.39

    TABLE-US-00006 TABLE 5 Peptide information on position and sequence of each peptide identified as IPM protein Oxi- Actual dation Charge SEQUEST SEQUEST Observed  mass value Start Stop Sequence site (z) XCorr deltaCn m/z (Da)  19  26 (A)AESLPDLK(I) 2 2.48 0.40  436.74  871.47  19  29 (A)AESLPDLKIEK(L) 2 3.47 0.57  621.85 1241.69  20  26 (A)ESLPDLK(I) 2 2.06 0.27  401.22  800.43  20  29 (A)ESLPDLKIEK(L) 2 3.20 0.53  586.33 1170.65  21  29 (E)SLPDLKIEK(L) 3 2.53 0.41  348.21 1041.61  22  29 (S)LPDLKIEK(L) 2 2.99 0.50  478.29  954.57  23  29 (L)PDLKIEK(L) 2 3.04 0.51  421.75  841.49  27  37 (K)IEKLDEGVYVH(T) 3 2.29 0.34  434.56 1300.67  27  51 (K)IEKLDEGVYVHTSFEEVNG 3 5.36 0.72  944.49 2830.44 WGVVPK(H)  30  37 (K)LDEGVYVH(T) 2 2.43 0.38  466.23  930.45  30  51 (K)LDEGVYVHTSFEEVNGWG 2 6.47 0.77 1231.11 2460.20 VVPK(H)  38  51 (H)TSFEEVNGWGVVPK(H) 2 2.67 0.44  774.89 1547.76  52  59 (K)HGLVVLVN(A) 2 1.88 0.20  425.76  849.51  52  72 (K)HGLVVLVNAEAYLIDTPFT 2 6.65 0.77 1136.13 2270.25 AK(D)  52  76 (K)HGLVVLVNAEAYLIDTPFT 2 5.68 0.74 1372.74 2743.46   AKDTEK(L)  58  72 (L)VNAEAYLIDTPFTAK(D) 2 3.34 0.55  826.93 1651.85  60  72 (N)AEAYLIDTPFTAK(D) 2 3.60 0.58  720.38 1438.74  60  76 (N)AEAYLIDTPFTAKDTEK(L) 2 4.60 0.67  956.98 1911.95  61  72 (A)EAYLIDTPFTAK(D) 2 1.97 0.24  684.86 1367.70  64  72 (Y)LIDTPFTAK(D) 2 1.84 0.19  503.29 1004.56  64  76 (Y)LIDTPFTAKDTEK(L) 2 3.50 0.57  739.90 1477.78  73  84 (K)DTEKLVTWFVER(G) 2 3.91 0.62  761.90 1521.79  73  87 (K)DTEKLVTWFVERGYK(I) 3 3.36 0.55  624.33 1869.97  75  84 (T)EKLVTWFVER(G) 2 2.62 0.43  653.86 1305.72  76  84 (E)KLVTWFVER(G) 2 2.32 0.35  589.34 1176.67  77  84 (K)LVTWFVER(G) 2 2.62 0.43  525.29 1048.57  77  85 (K)LVTWFVERG(Y) 2 2.43 0.38  553.81 1105.60  77  87 (K)LVTWFVERGYK(I) 2 2.49 0.40  699.39 1396.76  78  84 (L)VTWFVER(G) 2 1.85 0.19  468.75  935.49  88  99 (K)IKGSISSHFHSD(S) 3 3.27 0.54  438.89 1313.64  88 100 (K)IKGSISSHFHSDS(T) 3 2.29 0.35  467.90 1400.67  88 102 (K)IKGSISSHFHSDSTG(G) 3 2.29 0.34  520.59 1558.74  88 105 (K)IKGSISSHFHSDSTGGIE(W) 2 5.14 0.71  929.96 1857.90  88 110 (K)IKGSISSHFHSDSTGGIEWLN 3 8.19 0.82  839.08 2514.23   SR(S)  90  98 (K)GSISSHFHS(D) 2 1.88 0.20  479.73  957.44  90  99 (K)GSISSHFHSD(S) 3 1.85 0.19  358.49 1072.46  90 101 (K)GSISSHFHSDST(G) 2 2.83 0.47  631.28 1260.54  90 102 (K)GSISSHFHSDSTG(G) 2 3.51 0.57  659.79 1317.56  90 105 (K)GSISSHFHSDSTGGIE(W) 2 3.89 0.61  809.36 1616.71  90 110 (K)GSISSHFHSDSTGGIEWLNS 3 8.69 0.83  758.69 2273.05 R(S)  90 111 (K)GSISSHFHSDSTGGIEWLNS 3 2.00 0.25  787.70 2360.09 RS(I)  97 110 (F)HSDSTGGIEWLNSR(S) 2 4.66 0.68  779.87 1557.72  98 110 (H)SDSTGGIEWLNSR(S) 2 3.76 0.60  711.34 1420.67  99 110 (S)DSTGGIEWLNSR(S) 2 2.13 0.30  667.82 1333.63 100 110 (D)STGGIEWLNSR(S) 2 2.80 0.46  610.31 1218.61 103 110 (G)GIEWLNSR(S) 2 2.33 0.36  487.76  973.50 104 110 (G)IEWLNSR(S) 2 2.09 0.28  459.25  916.48 111 125 (R)SIPTYASELTNELLK(K) 2 4.29 0.65  839.95 1677.88 111 126 (R)SIPTYASELTNELLKK(D) 3 4.86 0.69  603.00 1805.98 111 127 (R)SIPTYASELTNELLKKD(G) 2 3.35 0.55  961.51 1921.02 111 129 (R)SIPTYASELTNELLKKDGK 3 4.29 0.65  703.05 2106.13 (V) 113 125 (I)PTYASELTNELLK(K) 2 4.75 0.68  739.89 1477.76 113 126 (I)PTYASELTNELLKK(D) 3 7.19 0.79  536.30 1605.87 113 129 (I)PTYASELTNELLKKDGK(V) 3 3.48 0.57  636.34 1906.00 114 126 (P)TYASELTNELLKK(D) 2 3.89 0.61  755.42 1508.82 115 126 (T)YASELTNELLKK(D) 3 1.96 0.24  470.26 1407.77 116 125 (Y)ASELTNELLK(K) 2 1.77 0.15  559.31 1116.61 116 126 (Y)ASELTNELLKK(D) 2 2.90 0.48  623.36 1244.70 116 129 (Y)ASELTNELLKKDGK(V) 2 3.63 0.59  773.43 1544.84 118 126 (S)ELTNELLKK(D) 3 1.83 0.18  363.22 1086.63 126 136 (K)KDGKVQATNSF(S) 2 1.99 0.25  597.81 1193.61 126 145 (K)KDGKVQATNSFSGVNYWL 2 5.39 0.72 1121.09 2240.16 VK(N) 127 136 (K)DGKVQATNSF(S) 2 2.04 0.26  533.76 1065.51 127 140 (K)DGKVQATNSFSGVN(Y) 2 2.68 0.44  712.35 1422.68 127 141 (K)DGKVQATNSFSGVNY(W) 2 3.35 0.55  793.88 1585.74 127 145 (K)DGKVQATNSFSGVNYWLV 2 6.57 0.77 1057.04 2112.07 K(N) 129 145 (G)KVQATNSFSGVNYWLVK 2 6.17 0.76  971.02 1940.03 (N) 130 136 (K)VQATNSF(S) 1 2.42 0.38  766.38  765.37 130 141 (K)VQATNSFSGVNY(W) 2 2.28 0.34  643.81 1285.60 130 145 (K)VQATNSFSGVNYWLVK(N) 2 5.47 0.73  906.97 1811.92 132 145 (Q)ATNSFSGVNYWLVK(N) 2 4.10 0.63  793.41 1584.80 133 145 (A)TNSFSGVNYWLVK(N) 2 3.14 0.52  757.89 1513.76 134 145 (T)NSFSGVNYWLVK(N) 2 2.64 0.43  707.36 1412.71 135 145 (N)SFSGVNYWLVK(N) 2 3.07 0.51  650.34 1298.67 137 145 (F)SGVNYWLVK(N) 1 2.26 0.34 1065.58 1064.57 146 157 (K)NKIEVFYPGPGH(T) 2 3.85 0.61  679.35 1356.68 146 158 (K)NKIEVFYPGPGHT(P) 2 2.75 0.45  729.88 1457.74 146 160 (K)NKIEVFYPGPGHTPD(N) 2 4.74 0.68  835.92 1669.82 146 161 (K)NKIEVFYPGPGHTPDN(V) 2 4.79 0.69  892.94 1783.86 146 169 (K)NKIEVFYPGPGHTPDNVVV 3 7.92 0.81  921.81 2762.41 WLPER(K) 146 170 (K)NKIEVFYPGPGHTPDNVVV 3 7.59 0.80  964.51 2890.52 WLPERK(I) 147 170 (N)KIEVFYPGPGHTPDNVVVW 3 1.79 0.16  926.50 2776.48 LPERK(I) 148 160 (K)IEVFYPGPGHTPD(N) 2 2.83 0.47  714.85 1427.68 148 161 (K)IEVFYPGPGHTPDN(V) 2 2.54 0.41  771.87 1541.72 148 169 (K)IEVFYPGPGHTPDNVVVWL 2 4.76 0.69 1261.15 2520.28 PER(K) 148 170 (K)IEVFYPGPGHTPDNVVVWL 3 5.72 0.74  883.80 2648.37 PERK(I) 152 170 (F)YPGPGHTPDNVVVWLPERK 3 1.99 0.25  721.04 2160.11 (I) 153 170 (Y)PGPGHTPDNVVVWLPERK 3 2.90 0.48  666.69 1997.05 (I) 155 169 (G)PGHTPDNVVVWLPER(K) 3 3.66 0.59  572.64 1714.89 157 169 (G)HTPDNVVVWLPER(K) 3 3.39 0.56  521.28 1560.81 158 169 (H)TPDNVVVWLPER(K) 2 4.30 0.65  712.88 1423.75 158 170 (H)TPDNVVVWLPERK(I) 2 2.16 0.31  776.93 1551.85 161 170 (D)NVVVWLPERK(I) 2 2.01 0.25  620.37 1238.72 162 169 (N)VVVWLPER(K) 2 2.01 0.25  499.30  996.58 162 170 (N)WVWLPERK(I) 2 2.25 0.33  563.35 1124.68 170 176 (R)KILFGGc(F) 2 1.99 0.25  397.72  793.42 170 177 (R)KILFGGcF(I) 2 1.76 0.15  471.25  940.49 170 179 (R)KILFGGcFIK(P) 2 3.85 0.61  591.84 1181.67 170 181 (R)KILFGGcFIKPY(G) 2 3.83 0.61  721.90 1441.79 170 185 (R)KILFGGcFIKPYGLGN(L) 2 4.54 0.67  892.49 1782.96 170 196 (R)KILFGGcFIKPYGLGNLGDA 3 3.74 0.60  993.53 2977.58 NIEAWPK(S) 171 181 (K)ILFGGcFIKPY(G) 2 2.71 0.45  657.85 1313.69 171 182 (K)ILFGGcFIKPYG(L) 2 1.99 0.25  686.36 1370.71 171 196 (K)ILFGGcFIKPYGLGNLGDANI 2 3.43 0.56 1425.75 2849.48 EAWPK(S) 177 196 (C)FIKPYGLGNLGDANIEAWPK 3 2.71 0.45  735.06 2202.16 (S) 178 196 (F)IKPYGLGNLGDANIEAWPK 2 3.24 0.54 1028.55 2055.08 (S) 180 196 (K)PYGLGNLGDANIEAWPK(S) 2 2.53 0.41  907.96 1813.91 182 196 (Y)GLGNLGDANIEAWPK(S) 2 4.68 0.68  777.90 1553.79 182 199 (Y)GLGNLGDANIEAWPKSAK 2 2.71 0.45  920.99 1839.96 (L) 184 196 (L)GNLGDANIEAWPK(S) 2 3.70 0.59  692.85 1383.69 186 196 (N)LGDANIEAWPK(S) 2 3.55 0.58  607.32 1212.62 205 225 (K)YGKAKLVVPGHSEVGDASL 3 5.90 0.75  723.41 2167.21 LK(L) 206 225 (Y)GKAKLVVPGHSEVGDASLL 3 3.58 0.58  669.06 2004.15 K(L) 208 220 (K)AKLVVPGHSEVGD(A) 2 2.98 0.50  654.35 1306.69 208 224 (K)AKLVVPGHSEVGDASLL(K) 2 3.35 0.55  846.48 1690.94 208 225 (K)AKLVVPGHSEVGDASLLK 2 5.43 0.72  910.52 1819.03 (L) 208 233 (K)AKLVVPGHSEVGDASLLKL 3 2.13 0.30  901.52 2701.54 TLEQAVK(G) 209 225 (A)KLVVPGHSEVGDASLLK(L) 3 5.50 0.73  583.67 1747.99 210 219 (K)LVVPGHSEVG(D) 2 1.83 0.18  497.27  992.53 210 220 (K)LVVPGHSEVGD(A) 2 2.52 0.41  554.79 1107.56 210 221 (K)LVVPGHSEVGDA(S) 2 1.89 0.21  590.31 1178.60 210 224 (K)LVVPGHSEVGDASLL(K) 2 2.71 0.45  746.90 1491.79 210 225 (K)LVVPGHSEVGDASLLK(L) 2 4.77 0.69  810.96 1619.90 210 226 (K)LVVPGHSEVGDASLLKL(T) 2 1.99 0.25  867.50 1732.98 210 233 (K)LVVPGHSEVGDASLLKLTL 3 3.22 0.53  835.14 2502.41 EQAVK(G) 211 225 (L)VVPGHSEVGDASLLK(L) 2 3.87 0.61  754.41 1506.81 212 225 (V)VPGHSEVGDASLLK(L) 2 5.14 0.71  704.88 1407.74 213 225 (V)PGHSEVGDASLLK(L) 2 5.48 0.73  655.34 1308.67 214 225 (P)GHSEVGDASLLK(L) 2 4.50 0.67  606.81 1211.61 215 225 (G)HSEVGDASLLK(L) 2 4.18 0.64  578.30 1154.59 216 225 (H)SEVGDASLLK(L) 2 2.52 0.41  509.78 1017.54 219 225 (V)GDASLLK(L) 2 2.38 0.37  352.20  702.39 225 233 (L)KLTLEQAVK(G) 2 3.47 0.57  515.32 1028.62 225 239 (L)KLTLEQAVKGLNESK(K) 2 4.55 0.67  829.48 1656.95 226 233 (K)LTLEQAVK(G) 2 2.44 0.38  451.27  900.53 226 239 (K)LTLEQAVKGLNESK(K) 2 4.86 0.69  765.43 1528.85 226 246 (K)LTLEQAVKGLNESKKPSKPS 4 6.74 0.78  567.82 2267.25 N(-) 227 233 (L)TLEQAVK(G) 2 2.31 0.35  394.73  787.45 227 246 (L)TLEQAVKGLNESKKPSKPSN 3 8.03 0.81  719.06 2154.17 (-) 228 246 (T)LEQAVKGLNESKKPSKPSN 3 5.99 0.75  685.38 2053.11 (-) 229 246 (L)EQAVKGLNESKKPSKPSN(-) 3 6.06 0.75  647.69 1940.03 230 246 (E)QAVKGLNESKKPSKPSN(-) 3 5.71 0.74  604.67 1810.98 231 246 (Q)AVKGLNESKKPSKPSN(-) 3 4.71 0.68  561.99 1682.94 232 246 (A)VKGLNESKKPSKPSN(-) 3 3.90 0.62  538.30 1611.89 234 246 (K)GLNESKKPSKPSN(-) 2 4.18 0.64  693.37 1384.73 238 246 (E)SKKPSKPSN(-) 2 2.49 0.40  486.78  971.54

    TABLE-US-00007 TABLE 6 Information on position and sequence of each peptide identified as VIM protein Actual Oxi- mass dation Charge SEQUEST SEQUEST Observed value Start Stop Sequence site (z) XCorr deltaCn m/z (Da)  27  45 (S)VDSSGEYPTVSEIPVGEVR(L) 2 5.08 0.70 1010.50 2018.99  28  45 (V)DSSGEYPTVSEIPVGEVR(L) 2 3.43 0.56  960.97 1919.92  29  45 (D)SSGEYPTVSEIPVGEVR(L) 2 4.55 0.67  903.45 1804.89  31  45 (S)GEYPTVSEIPVGEVR(L) 2 3.85 0.61  816.42 1630.83  33  45 (E)YPTVSEIPVGEVR(L) 2 2.45 0.39  723.39 1444.76  34  45 (Y)PTVSEIPVGEVR(L) 2 4.25 0.65  641.86 1281.70  34  46 (Y)PTVSEIPVGEVRL(Y) 2 2.80 0.47  698.40 1394.78  34  47 (Y)PTVSEIPVGEVRLY(Q) 2 4.39 0.66  779.93 1557.85  35  45 (P)TVSEIPVGEVR(L) 2 1.97 0.24  593.33 1184.65  36  45 (T)VSEIPVGEVR(L) 2 2.91 0.48  542.81 1083.60  37  45 (V)SEIPVGEVR(L) 2 1.74 0.14  493.27  984.53  46  56 (R)LYQIADGVWSH(I) 2 2.76 0.46  644.82 1287.63  46  60 (R)LYQIADGVWSHIATQ(S) 2 2.67 0.44  851.44 1700.86  55  62 (W)SHIATQSF(D) 2 2.15 0.30  445.72  889.43  55  67 (W)SHIATQSFDGAVY(P) 2 3.51 0.57  698.33 1394.65  55  72 (W)SHIATQSFDGAVYPSNGL(I) 2 4.66 0.68  932.45 1862.89  55  75 (W)SHIATQSFDGAVYPSNGLIVR(D) 2 4.76 0.68 1116.58 2231.14  57  75 (H)IATQSFDGAVYPSNGLIVR(D) 2 3.76 0.60 1004.53 2007.05  59  75 (A)TQSFDGAVYPSNGLIVR(D) 2 3.29 0.54  912.47 1822.92  60  75 (T)QSFDGAVYPSNGLIVR(D) 2 2.97 0.49  861.95 1721.88  63  75 (F)DGAVYPSNGLIVR(D) 2 3.00 0.50  680.87 1359.72  68  80 (Y)PSNGLIVRDGDEL(L) 2 3.43 0.56  692.86 1383.71  68  81 (Y)PSNGLIVRDGDELL(L) 2 5.05 0.70  749.40 1496.79  73  81 (L)IVRDGDELL(L) 2 2.27 0.34  515.28 1028.55  76  90 (R)DGDELLLIDTAWGAK(N) 2 3.70 0.59  808.92 1615.82  77  90 (D)GDELLLIDTAWGAK(N) 2 2.13 0.30  751.40 1500.79  81  87 (L)LLIDTAW(G) 1 1.63 0.08  831.46  830.46  81  90 (L)LLIDTAWGAK(N) 2 1.77 0.15  544.31 1086.61  88  96 (W)GAKNTAALL(A) 2 1.85 0.19  429.76  857.50  88 109 (W)GAKNTAALLAEIEKQIGLPVTR(A) 3 6.16 0.76  765.12 2292.33  89 101 (G)AKNTAALLAEIEK(Q) 3 2.13 0.29  457.94 1370.78  90 101 (A)KNTAALLAEIEK(Q) 2 4.46 0.66  650.88 1299.74  91 101 (K)NTAALLAEIEK(Q) 2 3.92 0.62  586.83 1171.65  91 109 (K)NTAALLAEIEKQIGLPVTR(A) 2 5.72 0.74 1019.10 2036.18  92 101 (N)TAALLAEIEK(Q) 2 3.40 0.56  529.81 1057.61  92 109 (N)TAALLAEIEKQIGLPVTR(A) 3 4.81 0.69  641.72 1922.13  93 101 (T)AALLAEIEK(Q) 1 2.41 0.38  957.56  956.56  95 101 (A)LLAEIEK(Q) 1 2.47 0.00  815.49  814.48  96 109 (L)LAEIEKQIGLPVTR(A) 2 3.17 0.53  783.97 1565.92  96 115 (L)LAEIEKQIGLPVTRAVSTHF(H) 2 5.38 0.72 1105.13 2208.25  97 109 (L)AEIEKQIGLPVTR(A) 3 2.86 0.47  485.29 1452.83  97 115 (L)AEIEKQIGLPVTRAVSTHF(H) 2 4.43 0.66 1048.58 2095.15  97 126 (L)AEIEKQIGLPVTRAVSTHFHDDRV 3 4.13 0.64 1086.92 3257.74 GGVDVL(R) 100 109 (I)EKQIGLPVTR(A) 2 2.16 0.30  570.84 1139.67 102 109 (K)QIGLPVTR(A) 2 2.81 0.47  442.27  882.53 102 119 (K)QIGLPVTRAVSTHFHDDR(V) 4 2.34 0.36  513.02 2048.07 103 109 (Q)IGLPVTR(A) 2 1.84 0.19  378.24  754.47 109 127 (T)RAVSTHFHDDRVGGVDVLR(A) 4 4.65 0.68  534.78 2135.11 110 117 (R)AVSTHFHD(D) 2 1.97 0.24  457.21  912.41 110 119 (R)AVSTHFHDDR(V) 2 3.67 0.59  592.78 1183.54 110 121 (R)AVSTHFHDDRVG(G) 2 1.88 0.20  670.82 1339.63 110 122 (R)AVSTHFHDDRVGG(V) 3 1.99 0.24  466.56 1396.66 110 124 (R)AVSTHFHDDRVGGVD(V) 2 2.94 0.49  806.38 1610.75 110 127 (R)AVSTHFHDDRVGGVDVLR(A) 2 3.95 0.62  990.51 1979.00 110 140 (R)AVSTHFHDDRVGGVDVLRAAGVA 4 1.86 0.19  803.91 3211.63 TYASPSTR(R) 111 119 (A)VSTHFHDDR(V) 2 2.62 0.43  557.26 1112.50 111 127 (A)VSTHFHDDRVGGVDVLR(A) 3 4.76 0.68  637.00 1907.97 112 119 (V)STHFHDDR(V) 2 2.67 0.44  507.72 1013.43 112 127 (V)STHFHDDRVGGVDVLR(A) 3 4.15 0.64  603.97 1808.90 113 127 (S)THFHDDRVGGVDVLR(A) 3 1.76 0.15  574.96 1721.87 114 127 (T)HFHDDRVGGVDVLR(A) 3 3.69 0.59  541.28 1620.82 115 127 (H)FHDDRVGGVDVLR(A) 2 2.80 0.46  742.88 1483.76 116 126 (F)HDDRVGGVDVL(R) 2 2.89 0.48  591.30 1180.59 116 127 (F)HDDRVGGVDVLR(A) 2 2.93 0.49  669.35 1336.69 116 134 (F)HDDRVGGVDVLRAAGVATY(A) 3 4.41 0.66  657.68 1970.00 116 140 (F)HDDRVGGVDVLRAAGVATYASPS 3 2.16 0.31  857.44 2569.29 TR(R) 117 127 (H)DDRVGGVDVLR(A) 2 2.87 0.48  600.82 1199.62 118 127 (D)DRVGGVDVLR(A) 3 2.35 0.36  362.54 1084.60 120 127 (R)VGGVDVLR(A) 2 2.83 0.47  407.74  813.47 120 140 (R)VGGVDVLRAAGVATYASPSTR(R) 3 4.02 0.63  683.04 2046.09 121 127 (V)GGVDVLR(A) 1 1.28 0.00  715.41  714.40 125 140 (D)VLRAAGVATYASPSTR(R) 3 2.14 0.30  540.64 1618.89 127 134 (L)RAAGVATY(A) 2 1.82 0.18  404.72  807.43 128 140 (R)AAGVATYASPSTR(R) 2 3.99 0.62  626.32  1250.63 128 141 (R)AAGVATYASPSIRR(L) 2 3.09 0.51  704.37 1406.73 129 140 (A)AGVATYASPSTR(R) 2 3.22 0.53  590.80 1179.59 129 141 (A)AGVATYASPSTRR(L) 2 1.87 0.20  668.85 1335.69 130 140 (A)GVATYASPSTR(R) 2 2.80 0.46  555.29 1108.56 130 141 (A)GVATYASPSTRR(L) 2 3.03 0.51  633.34 1264.66 131 140 (G)VATYASPSTR(R) 2 2.63 0.43  526.77 1051.53 132 141 (V)ATYASPSTRR(L) 2 2.27 0.34  555.29 1108.57 135 155 (Y)ASPSTRRLAEVEGNEIPTHSL(E) 4 2.34 0.36  566.80 2263.17 135 158 (Y)ASPSTRRLAEVEGNEIPTHSLEGL 4 4.13 0.64  641.58 2562.31 (S) 141 153 (R)RLAEVEGNEIPTH(S) 3 2.33 0.36  488.92 1463.74 141 155 (R)RLAEVEGNEIPTHSL(E) 3 2.98 0.50  555.63 1663.86 141 156 (R)RLAEVEGNEIPTHSLE(G) 2 2.51 0.40  897.46 1792.90 141 157 (R)RLAEVEGNEIPTHSLEG(L) 3 3.43 0.56  617.65 1849.92 141 158 (R)RLAEVEGNEIPTHSLEGL(S) 3 4.87 0.69  655.34 1963.01 141 163 (R)RLAEVEGNEIPTHSLEGLSSSGD(A) 3 3.95 0.62  799.73 2396.17 141 166 (R)RLAEVEGNEIPTHSLEGLSSSGDAV 4 8.05 0.81  681.60 2722.38 R(F) 142 155 (R)LAEVEGNEIPTHSL(E) 2 3.01 0.50  754.89 1507.76 142 158 (R)LAEVEGNEIPTHSLEGL(S) 2 2.70 0.44  904.47 1806.93 142 163 (R)LAEVEGNEIPTHSLEGLSSSGD(A) 2 3.54 0.58 1121.03 2240.05 142 166 (R)LAEVEGNEIPTHSLEGLSSSGDAVR 2 7.36 0.80 1284.13 2566.25 (F) 143 155 (L)AEVEGNEIPTHSL(E) 2 2.41 0.38  698.34 1394.67 143 158 (L)AEVEGNEIPTHSLEGL(S) 2 3.41 0.56  847.92 1693.82 143 166 (L)AEVEGNEIPTHSLEGLSSSGDAVR 2 4.68 0.68 1227.60 2453.19 (F) 143 167 (L)AEVEGNEIPTHSLEGLSSSGDAVRF 3 6.07 0.75  867.76 2600.25 (G) 144 166 (A)EVEGNEIPTHSLEGLSSSGDAVR(F) 3 5.10 0.71  795.05 2382.14 145 166 (E)VEGNEIPTHSLEGLSSSGDAVR(F) 3 5.67 0.74  752.04 2253.09 146 166 (V)EGNEIPTHSLEGLSSSGDAVR(F) 2 5.27 0.72 1078.02 2154.02 147 166 (E)GNEIPTHSLEGLSSSGDAVR(F) 3 5.07 0.70  676.00 2024.98 148 166 (G)NEIPTHSLEGLSSSGDAVR(F) 3 4.97 0.70  656.99 1967.96 149 166 (N)EIPTHSLEGLSSSGDAVR(F) 3 4.99 0.70  618.98 1853.91 151 166 (I)PTHSLEGLSSSGDAVR(F) 2 5.07 0.70  806.90 1611.79 153 166 (T)HSLEGLSSSGDAVR(F) 2 3.24 0.54  707.85 1413.69 154 166 (H)SLEGLSSSGDAVR(F) 2 3.10 0.52  639.32 1276.63 156 166 (L)EGLSSSGDAVR(F) 2 2.69 0.44  539.26 1076.51 156 167 (L)EGLSSSGDAVRF(G) 2 2.59 0.42  612.79 1223.57 157 166 (E)GLSSSGDAVR(F) 2 2.61 0.42  474.74  947.47 159 167 (L)SSSGDAVRF(G) 2 2.27 0.34  463.22  924.43 159 172 (L)SSSGDAVRFGPVEL(F) 2 2.68 0.44  710.86 1419.71 167 179 (R)FGPVELFYPGAAH(S) 2 3.73 0.60  702.85 1403.69 167 180 (R)FGPVELFYPGAAHS(T) 2 2.07 0.28  746.37 1490.73 167 182 (R)FGPVELFYPGAAHSTD(N) 2 2.59 0.42  854.41 1706.80 174 187 (F)YPGAAHSTDNLVVY(V) 2 2.45 0.39  753.87 1505.72 188 194 (Y)VPSASVL(Y) 1 2.07 0.28  672.39  671.39 188 201 (Y)VPSASVLYGGcAIY(E) 2 2.36 0.37  728.86 1455.71 191 205 (S)ASVLYGGcAIYELSR(T) 2 1.88 0.20  829.92 1657.82 192 205 (A)SVLYGGcAIYELSR(T) 2 2.70 0.44  794.40 1586.78 193 205 (S)VLYGGcAIYELSR(T) 2 3.12 0.52  750.88 1499.75 195 205 (L)YGGcAIYELSR(T) 2 3.62 0.59  644.81 1287.60 196 205 (Y)GGcAIYELSR(T) 2 2.09 0.28  563.27 1124.53 197 205 (G)GcAIYELSR(T) 2 2.29 0.35  534.76 1067.51 197 225 (G)GcAIYELSRTSAGNVADADLAEWP 3 3.45 0.56 1051.50 3151.48 TSIER(I) 199 205 (C)AIYELSR(T) 2 2.15 0.30  426.24  850.46 202 216 (Y)ELSRTSAGNVADADL(A) 2 3.79 0.60  759.88 1517.74 206 215 (R)TSAGNVADAD(L) 1 1.87 0.20  920.40  919.39 206 218 (R)TSAGNVADADLAE(W) 2 2.97 0.50  617.29 1232.56 206 225 (R)TSAGNVADADLAEWPTSIER(I) 2 6.34 0.76 1052.00 2101.99 208 225 (S)AGNVADADLAEWPTSIER(I) 2 2.78 0.46  957.97 1913.92 211 225 (N)VADADLAEWPTSIER(I) 2 4.37 0.66  836.92 1671.82 214 225 (D)ADLAEWPTSIER(I) 2 2.27 0.34  694.35 1386.68 216 225 (D)LAEWPTSIER(I) 2 3.20 0.53  601.32 1200.62 220 235 (W)PTSIERIQQHYPEAQF(V) 2 2.77 0.46  972.49 1942.96 226 234 (R)IQQHYPEAQ(F) 2 2.41 0.38  557.27 1112.53 226 239 (R)IQQHYPEAQFVIPG(H) 2 2.40 0.37  813.92 1625.84 226 240 (R)IQQHYPEAQFVIPGH(G) 3 4.16 0.64  588.64 1762.89 226 242 (R)IQQHYPEAQFVIPGHGL(P) 3 1.97 0.24  645.34 1932.99 226 244 (R)IQQHYPEAQFVIPGHGLPG(G) 3 2.41 0.38  696.70 2087.07 226 245 (R)IQQHYPEAQFVIPGHGLPGG(L) 3 2.70 0.45  715.71 2144.09 226 246 (R)IQQHYPEAQFVIPGHGLPGGL(D) 3 3.95 0.62  753.40 2257.18 226 247 (R)IQQHYPEAQFVIPGHGLPGGLD(L) 3 4.63 0.68  791.74 2372.20 226 248 (R)IQQHYPEAQFVIPGHGLPGGLDL(L) 3 2.67 0.44  829.44 2485.28 226 249 (R)IQQHYPEAQFVIPGHGLPGGLDLL 3 2.70 0.44  867.13 2598.37 (K) 226 250 (R)IQQHYPEAQFVIPGHGLPGGLDLL 3 7.74 0.81  909.83 2726.47 K(H) 226 254 (R)IQQHYPEAQFVIPGHGLPGGLDLL 4 2.63 0.43  795.93 3179.69 KHTTN(V) 226 257 (R)IQQHYPEAQFVIPGHGLPGGLDLL 5 3.45 0.57  702.19 3505.89 KHTTNVVK(A) 235 250 (Q)FVIPGHGLPGGLDLLK(H) 3 2.23 0.33  544.99 1631.95 236 246 (F)VIPGHGLPGGL(D) 2 1.98 0.24  508.80 1015.59 236 248 (F)VIPGHGLPGGLDL(L) 2 2.70 0.44  622.86 1243.70 236 250 (F)VIPGHGLPGGLDLLK(H) 2 3.04 0.51  743.45 1484.89 237 250 (V)IPGHGLPGGLDLLK(H) 2 3.93 0.62  693.91 1385.80 238 250 (I)PGHGLPGGLDLLK(H) 2 4.71 0.68  637.37 1272.72 241 250 (H)GLPGGLDLLK(H) 2 1.80 0.17  491.80  981.59 243 250 (L)PGGLDLLK(H) 2 2.19 0.32  406.75  811.48 251 257 (K)HTTNVVK(A) 2 2.44 0.38  399.73  797.44 251 262 (K)HTTNVVKAHTNR(S) 3 2.09 0.28  459.92 1376.74 251 266 (K)HTTNVVKAHTNRSVVE(-) 3 2.25 0.33  597.99 1790.95

    TABLE-US-00008 Table 7 Information on position and sequence of each peptide identified as GES protein Oxi- Actual dation Charge SEQUEST SEQUEST Observed mass Start Stop Sequence site (z) XCorr deltaCn m/z value (Da)  19  25 (A)SEKLTFK(T) 2 2.70 0.45  426.75  851.48  22  30 (K)LTFKTDLEK(L) 3 2.62 0.43  365.54 1093.61  22  33 (K)LTFKTDLEKLER(E) 3 4.22 0.64  498.29 1491.84  26  33 (K)TDLEKLER(E) 2 3.65 0.59  502.28 1002.54  26  35 (K)TDLEKLEREK(A) 3 2.55 0.41  420.90 1259.68  27  33 (T)DLEKLER(E) 2 2.31 0.35  451.75  901.49  34  55 (R)EKAAQIGVAIVDPQGEIVAGHR 4 7.82 0.81  565.31 2257.21 (M)  36  55 (K)AAQIGVAIVDPQGEIVAGHR(M) 2 6.48 0.77 1001.05 2000.08  38  55 (A)QIGVAIVDPQGEIVAGHR(M) 3 3.42 0.56  620.34 1858.01  39  55 (Q)IGVAIVDPQGEIVAGHR(M) 2 3.98 0.62  865.98 1729.94  40  55 (I)GVAIVDPQGEIVAGHR(M) 2 2.90 0.48  809.44 1616.86  41  55 (G)VAIVDPQGEIVAGHR(M) 3 4.05 0.63  520.95 1559.84  42  55 (V)AIVDPQGEIVAGHR(M) 2 4.01 0.63  731.40 1460.78  43  55 (A)IVDPQGEIVAGHR(M) 3 3.70 0.59  464.25 1389.73  44  55 (I)VDPQGEIVAGHR(M) 2 2.44 0.38  639.33 1276.65  45  55 (V)DPQGEIVAGHR(M) 2 2.91 0.48  589.80 1177.58  46  55 (D)PQGEIVAGHR(M) 2 3.02 0.50  532.28 1062.56  60  67 (R)FAmcSTFK(F) M(62) 2 2.60 0.42  504.22 1006.43  60  67 (R)FAMcSTFK(F) 2 2.15 0.30  496.22  990.44  60  77 (R)FAmcSTFKFPLAALVFER(I) M(62) 3 2.17 0.31  717.70 2150.08  61  67 (F)AMcSTFK(F) 1 1.54 0.03  844.37  843.36  68  77 (K)FPLAALVFER(I) 2 3.43 0.56  581.84 1161.66  70  77 (P)LAALVFER(I) 2 1.94 0.23  459.78  917.54  71  77 (L)AALVFER(I) 2 2.40 0.37  403.23  804.44  78  84 (R)IDSGTER(G) 2 2.17 0.31  389.19 776.37  78  87 (R)IDSGTERGDR(K) 3 2.03 0.26  369.18 1104.52  88 105 (R)KLSYGPDmIVEWSPATER(F) M(95) 3 4.89 0.69  699.01 2094.01  88 105 (R)KLSYGPDMIVEWSPATER(F) 3 3.35 0.55  693.68 2078.01  89 105 (K)LSYGPDmIVEWSPATER(F) M(95) 2 4.26 0.65  983.97 1965.92  89 105 (K)LSYGPDMIVEWSPATER(F) 3 2.26 0.34  650.98 1949.91 106 118 (R)FLASGHmTVLEAA(Q) M(112) 2 2.65 0.43  681.84 1361.66 106 118 (R)FLASGHMTVLEAA(Q) 2 2.68 0.44  673.84 1345.67 106 120 (R)FLASGHmTVLEAAQA(A) M(112) 3 3.34 0.55  521.26 1560.76 106 120 (R)FLASGHMTVLEAAQA(A) 3 3.39 0.56  515.93 1544.76 106 121 (R)FLASGHmTVLEAAQAA(V) M(112) 3 4.55 0.67  544.94 1631.80 106 121 (R)FLASGHMTVLEAAQAA(V) 3 2.84 0.47  539.61 1615.80 106 123 (R)FLASGHmTVLEAAQAAVQ(L) M(112) 3 3.89 0.61  620.65 1858.93 106 123 (R)FLASGHMTVLEAAQAAVQ(L) 3 2.07 0.28  615.32 1842.93 106 135 (R)FLASGHmTVLEAAQAAVQLSDN M(112) 3 3.22 0.53 1043.21 3126.59 GATNLLLR(E) 106 135 (R)FLASGHMTVLEAAQAAVQLSDN 3 2.64 0.43 1037.87 3110.60 GATNLLLR(E) 110 135 (S)GHmTVLEAAQAAVQLSDNGATN M(112) 3 3.49 0.57  903.80 2708.39 LLLR(E) 110 135 (S)GHMTVLEAAQAAVQLSDNGATN 3 4.30 0.65  898.47 2692.39 LLLR(E) 116 135 (L)EAAQAAVQLSDNGATNLLLR(E) 3 2.95 0.49  685.70 2054.07 119 135 (A)QAAVQLSDNGATNLLLR(E) 3 4.24 0.65  595.33 1782.96 121 135 (A)AVQLSDNGATNLLLR(E) 3 3.96 0.62  528.96 1583.86 122 135 (A)VQLSDNGATNLLLR(E) 3 3.38 0.56  505.28 1512.82 124 135 (Q)LSDNGATNLLLR(E) 3 2.56 0.42  429.57 1285.70 125 135 (L)SDNGATNLLLR(E) 2 3.40 0.56  587.32 1172.62 128 135 (N)GATNLLLR(E) 2 2.09 0.28  429.26  856.51 136 148 (R)EIGGPAAmTQYFR(K) M(143) 2 3.76 0.60  728.85 1455.68 136 148 (R)EIGGPAAMTQYFR(K) 2 3.68 0.59  720.85 1439.69 136 149 (R)EIGGPAAmTQYFRK(I) M(143) 2 3.26 0.54  792.90 1583.78 138 148 (I)GGPAAmTQYFR(K) M(143) 2 2.28 0.34  607.78 1213.55 149 156 (R)KIGDSVSR(L) 2 2.47 0.39  431.24  860.48 149 159 (R)KIGDSVSRLDR(K) 3 2.38 0.37  415.90 1244.69 150 159 (K)IGDSVSRLDR(K) 2 1.90 0.21  559.30 1116.59 157 173 (R)LDRKEPEmSDNTPGDLR(D) M(164) 3 5.69 0.74  663.65 1987.93 157 173 (R)LDRKEPEMSDNTPGDLR(D) 3 6.08 0.75  658.32 1971.93 160 173 (R)KEPEmSDNTPGDLR(D) M (164) 3 4.63 0.68  535.58 1603.73 160 173 (R)KEPEMSDNTPGDLR(D) 2 3.84 0.61  794.87 1587.72 160 181 (R)KEPEmSDNTPGDLRDTTTPIAM M(164) 3 2.39 0.37  812.37 2434.09 (A) 160 181 (R)KEPEmSDNTPGDLRDTTTPIAm M 3 1.82 0.18  817.70 2450.09 (A) (164), M(181) 160 183 (R)KEPEmSDNTPGDLRDTTTPIAMA M(164) 4 5.52 0.73  666.32 2661.25 R(T) 160 183 (R)KEPEmSDNTPGDLRDTTTPIAmA M 4 5.33 0.72  670.32 2677.24 R(T) (164), M(181) 160 183 (R)KEPEMSDNTPGDLRDTTTPIAMA 3 4.74 0.68  882.76 2645.24 R(T) 161 173 (K)EPEmSDNTPGDLR(D) M (164) 2 3.35 0.55  738.82 1475.63 161 173 (K)EPEMSDNTPGDLR(D) 2 2.84 0.47  730.82 1459.62 161 181 (K)EPEmSDNTPGDLRDTTTPIAM(A) M(164) 3 1.99 0.24  769.67 2306.00 161 183 (K)EPEMSDNTPGDLRDTTTPIAmAR M(181) 3 4.07 0.63  845.39 2533.14 (T) 161 183 (K)EPEmSDNTPGDLRDTTTPIAmAR M 4 3.75 0.60  638.29 2549.14 (T) (164), M(181) 161 183 (K)EPEMSDNTPGDLRDTTTPIAMAR 3 3.93 0.62  840.06 2517.15 (T) 174 183 (R)DTTTPIAmAR(T) M(181) 2 2.49 0.40  546.77 1091.52 174 183 (R)DTTTPIAMAR(T) 2 2.35 0.36  538.78 1075.54 184 204 (R)TVAKVLYGGALTSTSTHTIER(W) 3 3.36 0.55  735.73 2204.18 188 196 (K)VLYGGALTS(T) 1 2.08 0.28  880.48  879.47 188 197 (K)VLYGGALTST(S) 1 2.23 0.33  981.52  980.52 188 200 (K)VLYGGALTSTSTH(T) 2 2.92 0.49  653.83 1305.65 188 204 (K)VLYGGALTSTSTHTIER(W) 3 5.96 0.75  602.66 1804.95 188 217 (K)VLYGGALTSTSTHTIERWLIGNQ 4 2.09 0.28  808.67 3230.66 TGDATLR(A) 189 204 (V)LYGGALTSTSTHTIER(W) 2 3.77 0.60  853.94 1705.86 190 204 (L)YGGALTSTSTHTIER(W) 3 4.90 0.69  531.93 1592.77 191 204 (Y)GGALTSTSTHTIER(W) 2 4.04 0.63  715.87 1429.73 192 204 (G)GALTSTSTHTIER(W) 2 4.06 0.63  687.35 1372.68 193 204 (G)ALTSTSTHTIER(W) 3 1.81 0.17  439.56 1315.67 195 204 (L)TSTSTHTIER(W) 2 2.43 0.38  566.78 1131.55 205 217 (R)WLIGNQTGDATLR(A) 3 3.79 0.60  482.26 1443.74 206 217 (W)LIGNQTGDATLR(A) 2 2.56 0.41  629.84 1257.67 218 229 (R)AGFPKDWVVGEK(T) 3 3.90 0.62  444.90 1331.69 219 229 (A)GFPKDWVVGEK(T) 2 2.45 0.39  631.33 1260.65 220 229 (G)FPKDWVVGEK(T) 2 2.70 0.44  602.82 1203.63 221 229 (F)PKDWVVGEK(T) 2 3.42 0.56  529.29 1056.56 223 229 (K)DWVVGEK(T) 2 1.96 0.23  416.72  831.42 230 245 (K)TGTcANGGRNDIGFFK(A) 3 2.49 0.40  572.28 1713.81 239 245 (R)NDIGFFK(A) 2 2.68 0.44  420.72  839.42 239 249 (R)NDIGFFKAQER(D) 2 2.34 0.36  662.84 1323.67 246 256 (K)AQERDYAVAVY(T) 2 1.83 0.18  642.81 1283.61 246 261 (K)AQERDYAVAVYTTAPK(L) 3 4.80 0.69  594.98 1781.91 250 261 (R)DYAVAVYTTAPK(L) 2 3.62 0.59  649.84 1297.66 262 272 (K)LSAVERDELVA(S) 2 2.48 0.39  601.32 1200.63 262 275 (K)LSAVERDELVASVG(Q) 2 2.56 0.41  722.88 1443.75 262 276 (K)LSAVERDELVASVGQ(V) 3 1.99 0.25  524.94 1571.81 262 279 (K)LSAVERDELVASVGQVIT(Q) 3 1.78 0.16  629.34 1885.01 262 280 (K)LSAVERDELVASVGQVITQ(L) 3 3.75 0.60  672.03 2013.07 262 281 (K)LSAVERDELVASVGQVITQL(I) 3 1.76 0.15  709.73 2126.16 262 287 (K)LSAVERDELVASVGQVITQLILST 4 4.56 0.67  696.89 2783.53 DK(-) 273 287 (A)SVGQVITQLILSTDK(-) 2 2.98 0.50  801.46 1600.90 274 287 (S)VGQVITQLILSTDK(-) 3 2.82 0.47  505.63 1513.87 276 287 (G)QVITQLILSTDK(-) 2 2.76 0.46  679.90 1357.78 277 287 (Q)VITQLILSTDK(-) 2 3.08 0.51  615.87 1229.72 280 287 (T)QLILSTDK(-) 2 1.80 0.17  459.27  916.52 281 287 (Q)LILSTDK(-) 1 2.28 0.34  789.47  788.46

    [0137] For the KPC protein, a peptide in which one methionine among three methionine residues (49, 116 and 151) was in an oxidized form was identified (FIG. 6A). For OXA, a peptide in which one methionine or two or three methionine residues among six methionine residues (115, 138, 195, 237, 239 and 241) were in an oxidized form was identified (FIG. 6B).

    [0138] Specifically, for the KPC protein, an N-terminal sequence peptide consisting of residues 1-21 was not detected, and for the OXA protein, an N-terminal sequence peptide consisting of residues 1-22 was not detected. For this KPC, when the sequence except residues 1-21 was considered, peptides covering the entire sequence were identified, and the sequence coverage increased from 92.8% (272/293) to 100% (272/272). For OXA, when the sequence except residues 1-22 was considered, the sequence coverage increased from 91.7% (243/265) to 100% (243/243).

    [0139] For the NDM protein, a peptide in which one methionine among seven methionine residues (39, 67, 126, 129, 245, 248 and 265) was in an oxidized form was identified (FIG. 6E). For GES, a peptide in which one methionine or two methionine residues among six methionine residues (62, 95, 112, 143, 164 and 181) were in an oxidized form was identified (FIG. 6F). For IMP and VIM, a peptide in which methionine was in an oxidized form was not identified (FIGS. 6D and 6E).

    [0140] For the IMP and VIM proteins, N-terminal sequence peptides consisting of residues 1-18 and residues 1-26, respectively, were not detected, and for GES, an N-terminal sequence peptide consisting of residues 1-18 was not detected. When the target protein sequences excluding the N-terminus were considered, the identified sequence coverages were 97.2 (243/250) for NDM, 97.8% (223/228) for IMP, 100% (240/240) for VIM, and 98.5% (265/269) for GES.

    [0141] (3) Identification of N-Terminal Sequences (FIGS. 7A, 7B, 7C, 7D, 7E, 7F, 7G, 7H, 7I, 7J, 7K, 7L, 7M, 7N, 7O, 7P, 7Q, 7R, 7S and 7T)

    TABLE-US-00009 KPC -N-terminal sequence: (S)/ATALTNLVAEPFAK(L) (semi-tryptic) KPC-3 -N-terminal sequence: (S)/ATALTNLVAEPFAK(L) (semi-tryptic) KPC-17 -N-terminal sequence: (S)/ATALTNLVAEPFAK(L) (semi-tryptic) OXA -N-terminal sequence: (A)/KEWQENK(S) (semi-tryptic) OXA-181 -N-terminal sequence: (A)/KEWQENKSWNAHFTEHK(S) (semi-tryptic) NDM -N-terminal sequence: (M)PGEIRPTIGQQMETGDQR(F) (semi-tryptic) IMP -N-terminal sequence: (A)AESLPDLK(I) (semi-tryptic) IMP-1 -N-terminal sequence: (A)/AESLPDLK(I) (semi-tryptic) IMP-4 -N-terminal sequence: (A)/AESLPDLK(I) (semi-tryptic) VIM -N-terminal sequence: (S)/VDSSGEYPTVSEIPVGEVR(L) (semi-tryptic) VIM-1 -N-terminal sequence: (S)/GEPSGEYPTVNEIPVGEVR(L) (semi-tryptic) VIM-4 -N-terminal sequence: (S)/GEPSGEYPTVNEIPVGEVR(L) (semi-tryptic) GES -N-terminal sequence: (A)SEKLTFK(T) (semi-tryptic) GES-1 -N-terminal sequence: (A)/SEKLTFK(T) (semi-tryptic)

    Example 6: Amino Acid Sequencing and Characterization of Each Protein

    [0142] Based on the MS2 results obtained in Example 5, multiple alignment analysis was performed on a total of 43 KPC subtype proteins (FIG. 8A), 660 OXA subtype proteins (FIG. 8B), 27 NDM subtype proteins (FIG. 8C), 79 IMP subtype proteins (FIG. 8D), 66 VIM subtype proteins (FIG. 8E) and 43 GES subtype proteins (FIG. 8F) known to date in the National Center for Biotechnology Information (NCBI) database.

    [0143] KPC and OXA Proteins

    [0144] It was confirmed that 97.3% or more of the full-length amino acid sequences of 42 proteins including KPC-2 are conserved, and for the OXA protein, 91.3% or more of the full-length amino acid sequences of 30 proteins including OXA-48 having resistance to carbapenem antibiotics are conversed. In order to identify the characteristics of each protein, phylogenetic tree analysis of KPC and OXA was performed through the MEGA X program (FIGS. 9A and 9B). Specifically, 43 KPC subtype proteins all contained the same N-terminal peptide (1 to 21 a.a.), and among them, 35 subtype proteins including KPC-2 were each composed of a sequence consisting of 293 amino acids. For OXA, 30 OXA proteins comprising the same N-terminal peptide (1 to 22 a.a.) were identified, and 23 OXA proteins were characterized by the same sequence as OXA-48 composed of a sequence consisting of 265 amino acids.

    [0145] MBL Proteins and GES Protein

    [0146] It was confirmed that sequence similarities were 90.9% (NDM), 44.8% (IMP), 63.5% (VIM), and 86.5% (GES). As subtype proteins having the same N-terminus, 27 NDM subtype proteins, 23 IMP subtype proteins (89.4%), 26 VIM subtype proteins (92.1%), and 35 GES subtype proteins (87.9%) were identified. As subtype proteins having the same peptide number as the N-terminal sequence and the same full-length amino acid sequence, 26 NDM subtype proteins (92.6%), 23 IMP subtype proteins (89.4%), 25 VIM subtype proteins (93.6%), and 34 GES subtype proteins (88.9%) were identified. In order to identify the characteristics of each protein, phylogenetic tree analysis was performed on the subtype proteins of the MBL protein and the GES protein through the MEGA X program (FIGS. 9C, 9D, 9E and 9F). As a result, it could be confirmed that the subgroups each containing NDM-1, IMP-6, VIM-2 and GES-5 were grouped in the phylogenetic tree analysis, like the results confirmed in the multiple alignment analysis.

    Example 7. Target Protein Identification Using Mass Spectrometry (Top-Down Method)

    [0147] To determine the mass value of the active protein expressed in the strain, top-down mass spectrometry was performed. To confirm the exact mass value of the protein, a sample obtained by partial purification from the crude protein extract derived from the strain was used, and an LC-MS/MS system (Nano-LC and Q-Exactive HF-X mass spectrometry system) capable of top-down analysis was used.

    [0148] (1) Mass Spectrometry of Purified Protein

    [0149] About 0.5 μg of the partially purified protein sample was injected. Analysis was performed using a direct infusion method without a column and using a nano-flow pump. The sample analysis conditions used in this case are as follows. [0150] buffer A: 0.1% formic acid in water [0151] sample analysis: from 0 to 10 min, 100% fixed buffer (A) 100%, 4 μL/min fixed flow rate

    [0152] (2) Mass Spectrometry of Target Proteins Using High-Resolution Mass Spectrometry

    [0153] Using the protein mode analysis method with the Q-Exactive HF-X mass spectrometer, the mass values of the intact proteins and the tandem mass spectra of the proteins were obtained and identified. The parameters used in this case are as follows. [0154] Resolution: use of Full MS 120,000, MS2 60,000 or 120,000 [0155] Full MS: 620 to 2,400 m/z, 100 msec [0156] MS2: use of 1 or 2 microscans, 1,000 msec, NCE 50; ionized materials with a charge state of 1 to 8 were excluded from MS2 analysis.

    [0157] Software for identifying proteins based on top-down data, ‘Informed Proteomics’ developed by US PNNL (Pacific Northwest National Laboratory) was used.

    [0158] KPC and OXA Proteins

    [0159] Several multi-charged KPC protein (z=+13 to +20) or OXA protein (z=+22 to +37) peaks appeared (FIGS. 10A and 10B), and it was confirmed that a representative mass value obtained by deconvolution of the peaks was an average molecular weight of 28,718.13 m/z×z, corresponding to a monoisotopic mass of 28,700.69 m/z×z. The confirmed representative mass value represents the mass value of the KPC protein in a state in which there is a disulfide bond between Cys68 and Cys237. In addition, other peaks caused by methionine oxidation were observed (e.g., polypeptides with one oxidized methionine show an average molecular weight of 28,718+16 m/z×z). They partially coincided with the positions of oxidized methionine residues (49, 116 and 151 for KPC, and 115, 138, 195, 237, 239 and 241 for OXA) within the KPC or OXA protein sequence obtained through in-gel digestion (bottom-up method). Polypeptides including the N-terminal sequence consisting of residues 1-21 for KPC or residues 1-22 for OXA were not observed even in the analysis based on the top-down method. When each N-terminal sequence was excluded, both KPC (22-293 a.a.) and OXA (23-265 a.a.) showed a sequence coverage of 100% (FIGS. 12A and 12B).

    [0160] MBL Proteins and GES Protein

    [0161] Several peaks of multi-charged MBL proteins or GES protein appeared (FIGS. 10C, 10E, 10G and 10I), and it was confirmed that, for NDM, a representative mass value obtained by deconvolution of the peaks was an average molecular weight of 26,724 m/z X z, corresponding to a monoisotopic mass of 26,707 m/z×z. It was confirmed that the average molecular weight for IMP was 25082.68 m/z×z, corresponding to a monoisotopic mass of 25,067.19 m/z×z, the average molecular weight for VIM was 25,515.42 m/z×z, corresponding to a monoisotopic mass of 25,499.92 m/z×z, and the average molecular weight for GES was 29,245.15 m/z×z, corresponding to a monoisotopic mass of 29,226.92 m/z×z. It was confirmed that the identified representative mass value for NDM corresponded to a palmitoylated protein type (FIG. 11A). In addition, other peaks caused by methionine oxidation and methylation were observed (for example, polypeptides with one oxidized methionine show an average molecular weight of 26724+16 m/z×z, and polypeptides with one methylation show an average molecular weight of 26724+14 m/z x z) (FIG. 11B). On the other hand, for IMP and VIM, no specific protein modification was found. For GES, a disulfide bond between Cys63 and Cys233 was found, and other peaks caused by methionine oxidation were also observed. They partially coincided with the positions of oxidized methionine residues (39, 67, 126, 129, 245, 248 and 265 for NDM, and 62, 95, 112, 143, 164, 181 for GES) within the NDM or GES protein sequence obtained through in-gel digestion (bottom-up method). Polypeptides including the N-terminal sequence consisting of residues 1-19 or 1-20 for NDM, residues 1-18 for IMP, residues 1-26 for VIM, or residues 1-18 for GES were not observed even in the analysis based on the top-down method. When each N-terminal sequence was excluded, sequence coverage of 100% was identified in residues 21-270 for NDM protein (FIG. 12C), residues 19-246 for IMP protein (FIG. 12D), and residues 27-266 for VIM protein (FIG. 12E). In addition, for GES protein, sequence coverage of 100% was identified in residues 19-287 (FIG. 12F).

    [0162] (3) Mass Spectrometry of Target Proteins Using Low-Resolution Mass Spectrometry

    [0163] The mass spectrometry spectrum of each protein was obtained using a low-resolution mass spectrometer (1, SciEX 4800; 2, Bruker Biotyper MALDI-TOF MS system). First, 1 μL of a sinapinic acid (SA, present at 10 mg/mL in 0.1% TFA/50% acetonitrile) matrix and about 100 ng of each protein were placed on the plate spot, dried completely, and subjected to mass spectrometry. As protein samples, all of the colonies cultured in solid culture, cells harvested in liquid culture, crude extract and crude enzyme solution after cell lysis, and purified proteins obtained after purification can be used. In this case, the maximum energy used was 30%, random position acquisition was performed, a total of 2,000 laser shots (40 shots per time) were irradiated, and each spectral data was cumulatively obtained. Mass spectrometry spectra were obtained for the range of 10,000 to 40,000 m/z (KPC and OXA) or 35,000 m/z (MBL and GES), and each target protein with a charge state of +1 as well as each target protein with a charge state of +2 were simultaneously detected (FIGS. 13A, 13B, 13C, 13D, 13E and 13F). As a result of low-resolution mass spectrometry, both charge states of +1 and +2 were detected for all the three MBL proteins and the GES protein. In particular, for the NDM protein which is a protein anchored to the cell membrane, both a molecular weight corresponding to palmitoylation (average mass=238.4136, monoisotopic mass=238.22966) and a molecular weight corresponding to non-palmitoylation were identified.

    [0164] (4) Comparison of Mass Values of Protein Subtypes

    [0165] The exact mass values of the KPC, OXA, MBL and GES proteins were confirmed through the above-described method, and the mass values of the active proteins from which the N-terminal peptide has been removed could be confirmed based on the confirmed mass values. Thus, for all the KPC, OXA, MBL and GES proteins found in the NCBI, the exact mass values of the active proteins can be confirmed through high-resolution or low-resolution mass spectrometry, and it is possible to rapidly and accurately identify various types of subtype proteins through mass spectrometry (Tables 8 to 13).

    TABLE-US-00010 TABLE 8 Mass data of KPC protein Full-length KPC protein Full-length KPC protein (containing disulfide bond) Average Average molecular Monoisotopic molecular Monoisotopic Active protein Subtypes Da weight mass Da weight mass Da 2 31115 31115.35 31095.98 31113 31113.33 31093.96 28720 3 31141 31141.39 31121.99 31139 31139.37 31119.97 28746 4 31132 31132.34 31112.99 31130 31130.32 31110.97 28737 5 31174 31174.42 31155.03 31172 31172.40 31153.01 28780 6 31073 31073.27 31053.94 31071 31071.25 31051.92 28678 7 31123 31123.35 31104.03 31121 31121.33 31102.01 28728 8 31099 31099.30 31079.94 31097 31097.28 31077.92 28704 10 31200 31200.46 31181.04 31198 31198.44 31179.02 28806 11 31131 31131.39 31112.02 31129 31129.37 31110.00 28736 12 31133 31133.38 31113.94 31131 31131.36 31111.92 28738 13 31083 31083.35 31063.98 31081 31081.33 31061.96 28688 14 30957 30957.19 30937.92 30955 30955.17 30935.90 28562 15 31272 31271.58 31252.11 31270 31269.56 31250.09 28877 16 31071 31071.29 31051.98 31069 31069.27 31049.96 28676 17 31081 31081.33 31062.00 31079 31079.31 31059.98 28686 18 31129 31129.38 31110.00 31127 31127.36 31107.98 28720 19 31128 31128.39 31108.99 31126 31126.37 31106.97 28733 21 31085 31085.32 31066.01 31083 31083.30 31063.99 28690 22 30952 30952.17 30932.94 30950 30950.15 30930.92 28557 23 31113 31113.33 31093.96 31111 31111.31 31091.94 28718 24 31056 31056.28 31036.94 31054 31054.26 31034.92 28720 25 31358 31357.63 31338.11 31356 31355.61 31336.09 28963 26 31131 31131.35 31111.98 31129 31129.33 31109.96 28720 27 31111 31111.36 31092.01 31109 31109.34 31089.99 28716 28 30983 30983.23 30963.92 30981 30981.21 30961.90 28588 29 31500 31499.74 31480.14 31498 31497.72 31478.12 29105 30 31096 31096.30 31076.94 31094 31094.28 31074.92 28720 31 31189 31189.47 31170.03 31187 31187.45 31168.01 28795 32 31220 31219.56 31200.02 31218 31217.54 31198.00 28825 33 31163 31163.44 31144.02 31161 31161.42 31142.00 28769 34 32026 32026.29 32006.40 32024 32024.27 32004.38 29631 35 31099 31099.31 31079.95 31097 31097.29 31077.93 28704 36 31155 31155.41 31136.00 31153 31153.39 31133.98 28760 37 31051 31051.31 31032.02 31049 31049.29 31030.00 28656 38 31113 31113.33 31093.96 31111 31111.31 31091.94 28718 39 31171 31171.41 31152.00 31169 31169.39 31149.98 28776 40 31370 31369.63 31350.10 31368 31367.61 31348.08 28975 41 31481 31480.78 31461.18 31479 31478.76 31459.16 29086 42 31085 31085.32 31065.97 31083 31083.30 31063.95 28690 43 31143 31143.41 31124.03 31141 31141.39 31122.01 28748 44 32828 32828.19 32807.81 32826 32826.17 32805.79 30433 45 31142 31142.42 31123.03 31140 31140.40 31121.01 28748 46 31125 31125.34 31105.96 31123 31123.32 31103.94 28730 Active protein Active protein (containing disulfide bond) Average Average molecular Monoisotopic molecular Monoisotopic Subtypes weight mass Da weight mass N-terminus 2 28720.43 28702.71 28718 28718.41 28700.69 1~21aa 3 28746.46 28728.71 28744 28744.44 28726.69 1~21aa 4 28737.42 28719.71 28735 28735.40 28717.69 1~21aa 5 28779.50 28761.76 28777 28777.48 28759.74 1~21aa 6 28678.35 28660.66 28676 28676.33 28658.64 1~21aa 7 28728.43 28710.76 28726 28726.41 28708.74 1~21aa 8 28704.38 28686.67 28702 28702.36 28684.65 1~21aa 10 28805.53 28787.76 28804 28803.51 28785.74 1~21aa 11 28736.47 28718.74 28734 28734.45 28716.72 1~21aa 12 28738.46 28720.67 28736 28736.44 28718.65 1~21aa 13 28688.43 28670.71 28686 28686.41 28668.69 1~21aa 14 28562.27 28544.64 28560 28560.25 28542.62 1~21aa 15 28876.66 28858.84 28875 28874.64 28856.82 1~21aa 16 28676.37 28658.70 28674 28674.35 28656.68 1~21aa 17 28686.41 28668.73 28684 28684.39 28666.71 1~21aa 18 28720.43 28702.71 28718 28718.41 28700.69 1~21aa 19 28733.47 28715.72 28731 28731.45 28713.70 1~21aa 21 28690.40 28179.59 28688 28688.38 28177.57 1~21aa 22 28557.25 28539.67 28555 28555.23 28537.65 1~21aa 23 28718.41 28700.68 28716 28716.39 28698.66 1~21aa 24 28720.43 28702.71 28718 28718.41 28700.69 1~21aa 25 28962.70 28944.84 28961 28960.68 28942.82 1~21aa 26 28720.43 28702.71 28718 28718.41 28700.69 1~21aa 27 28716.44 28698.74 28714 28714.42 28696.72 1~21aa 28 28588.31 28570.65 28586 28586.29 28568.63 1~21aa 29 29104.82 29086.86 29103 29102.80 29084.84 1~21aa 30 28720.43 28702.71 28718 28718.41 28700.69 1~21aa 31 28794.55 28776.75 28793 28792.53 28774.73 1~21aa 32 28824.64 28806.74 28823 28822.62 28804.72 1~21aa 33 28768.52 28750.75 28767 28766.50 28748.73 1~21aa 34 29631.37 29613.12 29629 29629.35 29611.10 1~21aa 35 28704.39 28686.68 28702 28702.37 28684.66 1~21aa 36 28760.49 28742.73 28758 28758.47 28740.71 1~21aa 37 28656.39 28638.75 28654 28654.37 28636.73 1~21aa 38 28718.41 28700.68 28716 28716.39 28698.66 1~21aa 39 28776.49 28758.72 28774 28774.47 28756.70 1~21aa 40 28974.71 28956.83 28973 28972.69 28954.81 1~21aa 41 29085.86 29067.90 29084 29083.84 29065.88 1~21aa 42 28690.40 28672.70 28688 28688.38 28670.68 1~21aa 43 28748.49 28730.75 28746 28746.47 28728.73 1~21aa 44 30433.27 30414.54 30431 30431.25 30412.52 1~21aa 45 28747.50 28729.76 28745 28745.48 28727.74 1~21aa 46 28730.42 28712.68 28728 28728.40 28710.66 1~21aa

    TABLE-US-00011 TABLE 9 Mass data of OXA protein Full-length OXA protein Active protein Average Average molecular Monoisotopic molecular Monoisotopic Subtypes Da weight mass Da weight mass N-terminus 48 30359 30358.76 30339.55 28147 28146.97 28129.28 1~22aa 54 30280 30279.68 30260.45 28098 28097.89 28080.23 1~22aa 162 30329 30328.74 30309.54 28117 28116.94 28099.27 1~22aa 163 29891 29891.19 29872.27 27679 27679.40 27662.00 1~22aa 181 30313 30312.74 30293.55 28172 28172.02 28154.31 1~22aa 199 30299 30298.71 30279.52 28158 28157.99 28140.29 1~22aa 204 30423 30422.85 30403.60 28282 28282.14 28264.37 1~22aa 232 30244 30243.63 30224.48 28032 28031.83 28014.21 1~22aa 244 30260 30259.63 30240.47 28048 28047.83 28030.20 1~22aa 245 30393 30392.82 30373.57 28252 28252.11 28234.34 1~22aa 247 29814 29814.11 29795.25 27673 27673.39 27656.02 1~22aa 252 30331 30330.71 30311.52 28190 28189.99 28172.29 1~22aa 370 30401 30400.80 30381.56 28260 28260.08 28242.33 1~22aa 405 29859 29859.19 29840.28 27718 27718.48 27701.04 1~22aa 416 30401 30400.84 30381.60 28260 28260.13 28242.37 1~22aa 436 30285 30284.74 30265.51 28126 28125.97 28108.35 1~22aa 438 30111 30111.42 30092.35 27900 27899.62 27882.08 1~22aa 439 29865 29865.16 29846.26 27653 27653.36 27635.99 1~22aa 484 30214 30213.60 30194.47 28002 28001.80 27984.20 1~22aa 505 30389 30388.79 30369.56 28147 28146.97 28129.28 1~22aa 514 30387 30386.82 30367.58 28175 28175.02 28157.31 1~22aa 515 30361 30360.73 30341.53 28149 28148.94 28131.26 1~22aa 517 30088 30088.47 30069.42 27877 27876.68 27859.15 1~22aa 519 30373 30372.79 30353.57 28161 28160.99 28143.30 1~22aa 535 30185 30184.62 30165.45 27955 27954.78 27937.26 1~22aa 538 30363 30362.75 30343.53 28151 28150.96 28133.26 1~22aa 546 30343 30342.76 30323.56 28131 28130.97 28113.29 1~22aa 547 30427 30426.88 30407.63 28215 28215.09 28197.36 1~22aa 566 30403 30402.77 30383.54 28191 28190.98 28173.27 1~22aa 567 30190 30189.58 30170.47 27978 27977.78 27960.2 1~22aa

    TABLE-US-00012 TABLE 10 Mass data of NDM protein Active protein Active protein Full-length NDM protein (N-terminal residues 1-19) (N-terminal residues 1-20) Average Average Average molecular Monoisotopic molecular Monoisotopic molecular Monoisotopic Subtypes Da weight mass Da weight mass Da weight mass 1 28499 28499.46 28481.22 26510 26510.04 26493.16 26439 26438.96 26422.12 2 28473 28473.42 28455.20 26484 26484.00 26467.14 26413 26412.92 26396.10 3 28498 28498.48 28480.23 26509 26509.05 26492.17 26438 26437.98 26421.14 4 28481 28481.43 28463.26 26492 26492.01 26475.20 26421 26420.93 26404.16 5 28495 28495.45 28477.27 26506 26506.03 26489.22 26435 26434.95 26418.18 6 28528 28527.51 28509.25 26538 26538.09 26521.19 26467 26467.01 26450.15 7 28480 28480.44 28462.27 26491 26491.02 26474.22 26420 26419.94 26403.18 8 28423 28423.39 28405.25 26434 26433.97 26417.19 26363 26362.89 26346.16 9 28499 28498.52 28480.27 26509 26509.10 26492.21 26438 26438.02 26421.17 10 28648 28647.58 28629.25 26658 26658.15 26641.19 26587 26587.08 26570.16 11 28467 28467.40 28449.24 26478 26477.98 26461.18 26407 26406.90 26390.15 12 28539 28539.46 28521.26 26550 26550.04 26533.21 26479 26478.96 26462.17 13 28480 28480.44 28462.27 26491 26491.02 26474.22 26420 26419.94 26403.18 14 28441 28441.42 28423.21 26452 26452.00 26435.15 26381 26380.92 26364.11 15 28509 28509.48 28491.29 26520 26520.06 26503.23 26449 26448.98 26432.19 16 28480 28480.41 28462.17 26491 26490.99 26474.11 26420 26419.91 26403.08 17 28495 28494.51 28476.33 26505 26505.09 26488.27 26434 26434.01 26417.23 18 29103 29103.10 29084.49 27114 27113.67 27096.43 27043 27042.60 27025.40 19 28509 28508.50 28490.31 26519 26519.07 26502.25 26448 26448.00 26431.21 20 28476 28476.41 28458.23 26487 26486.99 26470.17 26416 26415.91 26399.14 21 28525 28525.48 28507.28 26536 26536.06 26519.23 26465 26464.98 26448.19 22 28481 28481.43 28463.26 26492 26492.01 26475.20 26421 26420.93 26404.16 23 28499 28499.46 28481.22 26510 26510.04 26493.16 26439 26438.96 26422.12 24 28513 28513.49 28495.23 26524 26524.07 26507.17 26453 26452.99 26436.14 25 28515 28515.46 28497.21 26526 26526.04 26509.15 26455 26454.96 26438.11 27 28527 28526.53 28508.26 26537 26537.11 26520.20 26466 26466.03 26449.17 28 28528 28527.51 28509.25 26538 26538.09 26521.19 26467 26467.01 26450.15

    TABLE-US-00013 TABLE 11 Mass data of IMP protein Full-length IMP protein Active form of IMP protein Average Average molecular Monoisotopic molecular Monoisotopic Subtypes Da weight mass Da weight mass N-terminus 1 27120 27120.19 27103.24 25113 25112.71 25097.2 1~18aa 2 27180 27180.25 27163.13 25151 25150.65 25135.08 1~19aa 3 27018 27018.1 27001.21 25011 25010.62 24995.17 1~18aa 4 27087 27087.2 27070.24 25080 25079.72 25064.2 1~18aa 5 27176 27176.24 27159.12 25020 25019.56 25004.05 1~19aa 6 27090 27090.16 27073.23 25083 25082.68 25067.19 1~18aa 7 27134 27134.24 27117.21 25025 25024.6 25009.15 1~19aa 8 27053 27053.06 27036.02 24952 24952.38 24936.94 1~20aa 9 27277 27277.22 27260.01 25192 25191.61 25175.97 1~18aa 10 27168 27168.23 27151.24 25161 25160.75 25145.2 1~18aa 11 27061 27061.01 27044.05 24973 24973.34 24957.93 1~19aa 12 27101 27101.29 27084.27 24988 24987.6 24972.09 1~19aa 13 27212 27212.26 27195.02 25078 25077.56 25061.95 1~20aa 14 27441 27440.57 27423.32 25365 25364.9 25349.23 1~18aa 15 27139 27139.07 27121.93 25011 25011.44 24995.94 1~19aa 16 27298 27298.24 27281.06 25268 25267.62 25251.96 1~18aa 17 27141 27141.14 27123.95 25006 25006.44 24990.88 1~20aa 18 27199 27199.28 27182.22 25186 25185.69 25170.14 1~18aa 19 27095 27095.14 27078.06 24994 24994.46 24978.98 1~20aa 20 27143 27143.19 27126.06 25043 25042.5 25026.98 1~20aa 21 27033 27032.96 27016.02 24945 24945.29 24929.9 1~19aa 22 27224 27224.16 27207.03 25208 25207.56 25191.95 1~18aa 23 27101 27101.11 27084.02 25000 25000.42 24984.94 1~20aa 24 27081 27081.08 27064.02 24980 24980.39 24964.94 1~20aa 25 27120 27120.19 27103.24 25113 25112.71 25097.2 1~18aa 26 27135 27135.24 27118.24 25128 25127.76 25112.2 1~18aa 27 27303 27303.26 27286.12 25216 25215.59 25200 1~19aa 28 27139 27139.19 27122.09 24983 24982.5 24967.02 1~19aa 29 27219 27219.17 27202.09 25115 25114.53 25098.96 1~19aa 30 27119 27119.25 27102.29 25112 25111.77 25096.25 1~18aa 31 27176 27176.19 27159.15 25116 25115.56 25100.01 1~18aa 32 27490 27489.64 27472.34 25414 25413.98 25398.25 1~18aa 33 27211 27211.26 27194.09 25205 25204.69 25189.08 1~18aa 34 27048 27048.12 27031.22 25041 25040.64 25025.18 1~18aa 35 27257 27257.13 27240.07 25139 25139.45 25123.91 1~19aa 37 27388 27388.44 27371.1 25254 25253.74 25238.03 1~20aa 38 27057 27057.17 27040.23 25050 25049.69 25034.19 1~18aa 39 27011 27011.07 26994.03 24910 24910.39 24894.95 1~20aa 40 27108 27108.13 27091.2 25101 25100.65 25085.16 1~18aa 41 27109 27109.05 27092.05 25021 25021.38 25005.93 1~19aa 42 27219 27219.32 27202.32 25212 25211.84 25196.28 1~18aa 43 27182 27182.28 27165.21 25073 25072.65 25057.15 1~19aa 44 27049 27048.96 27032.01 24961 24961.29 24945.9 1~19aa 45 27247 27247.2 27230 25105 25104.53 25088.94 1~19aa 46 26841 26840.97 26824.13 24831 24831.37 24816.02 1~18aa 48 27429 27428.51 27411.29 25353 25352.85 25337.19 1~18aa 49 27247 27247.32 27230.22 25234 25233.73 25218.14 1~18aa 51 27104 27104.21 27087.2 24995 24994.58 24979.14 1~19aa 52 27078 27078.11 27061.19 25071 25070.63 25055.15 1~18aa 53 27237 27237.16 27219.98 25094 25094.49 25078.92 1~19aa 54 27427 27426.54 27409.31 25351 25350.88 25335.21 1~18aa 55 27147 27147.34 27130.36 25174 25173.88 25158.3 1~18aa 56 27169 27169.25 27152.21 25156 25155.66 25140.13 1~18aa 58 27272 27272.2 27255.03 25199 25198.56 25182.93 1~19aa 59 27136 27136.27 27119.26 25129 25128.79 25113.22 1~18aa 60 27119 27119.25 27102.29 25112 25111.77 25096.25 1~18aa 61 27119 27119.24 27102.28 25112 25111.76 25096.24 1~18aa 62 27109 27109.04 27091.92 24981 24981.42 24965.93 1~19aa 63 27131 27131.31 27114.28 25018 25017.62 25002.1 1~19aa 64 27317 27317.29 27300.14 25216 25215.59 25200 1~19aa 65 27411 27410.54 27393.31 25335 25334.88 25319.22 1~18aa 66 27168 27168.23 27151.24 25161 25160.75 25145.2 1~18aa 67 27319 27319.26 27302.11 25232 25231.59 25216 1~19aa 68 27031 27030.98 27014.04 24872 24872.24 24856.89 1~20aa 69 27083 27083.09 27066.03 24982 24982.4 24966.95 1~20aa 70 27120 27120.19 27103.24 25113 25112.71 25097.2 1~18aa 71 27217 27217.3 27200.21 25204 25203.71 25188.13 1~18aa 73 27106 27106.18 27089.18 24796 24796.35 24781.04 1~21aa 74 27346 27346.28 27329.06 25259 25258.61 25242.94 1~19aa 75 27227 27227.34 27210.23 25214 25213.74 25198.15 1~18aa 76 27092 27092.13 27075.21 25085 25084.65 25069.17 1~18aa 77 27142 27142.15 27125.19 25135 25134.67 25119.15 1~18aa 78 27138 27138.21 27121.23 25131 25130.73 25115.19 1~18aa 79 27148 27148.2 27131.25 25141 25140.72 25125.21 1~18aa 80 27152 27152.23 27135.25 25145 25144.75 25129.2 1~18aa 82 27255 27255.22 27238.03 25113 25112.56 25096.98 1~19aa 83 27185 27185.25 27168.21 25172 25171.66 25156.13 1~18aa 84 27260 27260.31 27243.02 25126 25125.61 25109.95 1~20aa 85 27146 27146.22 27129.11 24990 24989.53 24974.04 1~19aa

    TABLE-US-00014 TABLE 12 Mass data of VIM protein Full-length VIM protein Active form of VIM protein Average Average molecular Monoisotopic molecular Monoisotopic Subtypes Da weight mass Da weight mass N-terminus 1 28024 28024.46 28007.22 25322 25322.13 25306.78 1~26aa 2 28327 28326.94 28309.51 25515 25515.42 25499.92 1~26aa 3 28300 28299.96 28282.53 25488 25488.44 25472.94 1~26aa 4 28094 28093.57 28076.29 25391 25391.24 25375.85 1~26aa 5 28042 28041.57 28024.32 25339 25339.25 25323.88 1~26aa 6 28328 28327.97 28310.54 25516 25516.45 25500.95 1~26aa 7 28112 28111.92 28094.63 25464 25463.74 25448.26 1~25aa 8 28297 28296.91 28279.5 25485 25485.39 25469.91 1~26aa 9 28339 28338.99 28321.54 25527 25527.47 25511.95 1~26aa 10 28343 28342.94 28325.5 25531 25531.42 25515.91 1~26aa 11 28300 28299.91 28282.5 25488 25488.39 25472.91 1~26aa 12 28117 28116.55 28099.25 25414 25414.23 25398.81 1~26aa 13 28220 28219.63 28202.29 25455 25455.24 25439.83 1~26aa 14 28124 28123.59 28106.3 25421 25421.27 25405.86 1~26aa 15 28311 28310.94 28293.51 25499 25499.42 25483.92 1~26aa 16 28353 28353.02 28335.56 25542 25541.5 25525.97 1~26aa 17 28345 28344.97 28327.46 25515 25515.42 25499.92 1~26aa 18 27941 27940.58 27923.37 25129 25129.06 25113.79 1~26aa 19 28108 28107.64 28090.35 25405 25405.31 25389.9 1~26aa 20 28346 28345.98 28328.55 25534 25534.46 25518.96 1~26aa 23 28258 28257.83 28240.44 25446 25446.31 25430.85 1~26aa 24 28284 28283.91 28266.49 25472 25472.39 25456.9 1~26aa 25 28147 28146.76 28129.42 25444 25444.43 25428.98 1~26aa 26 28000 28000.47 27983.25 25298 25298.15 25282.81 1~26aa 27 28040 28040.46 28023.22 25338 25338.13 25322.78 1~26aa 28 28070 28069.58 28052.32 25367 25367.26 25351.88 1~26aa 29 28058 28057.57 28040.32 25355 25355.25 25339.88 1~26aa 30 28354 28353.96 28336.52 25542 25542.44 25526.93 1~26aa 31 28320 28319.95 28302.54 25508 25508.43 25492.96 1~26aa 32 27966 27966.42 27949.22 25264 25264.1 25248.78 1~26aa 33 28008 28008.46 27991.23 25306 25306.13 25290.79 1~26aa 34 28038 28038.48 28021.24 25336 25336.16 25320.8 1~26aa 35 28054 28054.48 28037.23 25352 25352.16 25336.79 1~26aa 36 28355 28354.99 28337.55 25543 25543.47 25527.96 1~26aa 37 28110 28109.56 28092.29 25407 25407.24 25391.85 1~26aa 38 28052 28051.59 28034.4 25367 25367.3 25351.91 1~26aa 39 27970 27970.45 27953.24 25268 25268.12 25252.8 1~26aa 40 28122 28121.62 28104.32 25419 25419.29 25403.88 1~26aa 41 28326 28325.95 28308.52 25514 25514.43 25498.93 1~26aa 42 28038 28038.48 28021.24 25336 25336.16 25320.8 1~26aa 43 28122 28121.62 28104.32 25391 25391.24 25375.85 1~26aa 44 28313 28312.87 28295.46 25501 25501.35 25485.87 1~26aa 45 28339 28338.99 28321.54 25527 25527.47 25511.95 1~26aa 46 28333 28332.9 28315.46 25487 25487.36 25471.89 1~26aa 47 28240 28239.62 28222.26 25455 25455.24 25439.83 1~26aa 48 28361 28360.96 28343.49 25515 25515.42 25499.92 1~26aa 49 28058 28057.57 28040.32 25355 25355.25 25339.88 1~26aa 50 28257 28256.88 28239.48 25445 25445.36 25429.89 1~26aa 51 28355 28354.99 28337.54 25543 25543.47 25527.95 1~26aa 52 28044 28043.5 28026.27 25341 25341.18 25325.82 1~26aa 53 28210 28209.74 28192.41 25487 25487.36 25471.89 1~26aa 54 28067 28066.54 28049.28 25364 25364.22 25348.84 1~26aa 55 28127 28126.68 28109.39 25424 25424.36 25408.95 1~26aa 56 28243 28242.82 28225.44 25458 25458.37 25442.9 1~26aa 57 28024 28024.46 28007.22 25322 25322.13 25306.78 1~26aa 58 28304 28303.9 28286.47 25458 25458.37 25442.9 1~26aa 59 28044 28043.5 28026.27 25341 25341.18 25325.82 1~26aa 60 28303 28302.96 28285.53 25491 25491.44 25475.94 1~26aa 61 28161 28160.99 28143.65 25513 25512.82 25497.28 1~25aa 62 28355 28354.99 28337.54 25515 25515.42 25499.92 1~26aa 63 28327 28326.94 28309.51 25515 25515.42 25499.92 1~26aa 64 28051 28050.54 28033.28 25348 25348.21 25332.83 1~26aa 65 28327 28326.94 28309.51 25515 25515.42 25499.92 1~26aa 66 28329 28329.04 28311.58 25518 25517.52 25502 1~26aa 67 28299 28298.88 28281.48 25487 25487.36 25471.89 1~26aa 68 28053 28052.51 28035.26 25350 25350.19 25334.81 1~26aa

    TABLE-US-00015 TABLE 13 Mass data of GES protein Full-length GES protein Full-length GES protein (containing disulfide bond) Average Average molecular Monoisotopic molecular Monoisotopic Subtypes Da weight mass Da weight mass 1 31154 31154.48 31134.97 31152 31152.46 31132.95 2 31212 31211.53 31191.99 31210 31209.51 31189.97 3 31123 31123.45 31104.03 31121 31121.43 31102.01 4 31153 31153.48 31134.04 31151 31151.46 31132.02 5 31185 31184.50 31164.98 31182 31182.48 31162.96 6 31184 31183.56 31164.03 31182 31181.54 31162.01 7 31154 31153.54 31134.02 31152 31151.52 31132.00 8 31197 31196.56 31177.01 31195 31194.54 31174.99 9 31185 31184.50 31164.98 31182 31182.48 31162.96 10 31128 31128.40 31108.91 31126 31126.38 31106.89 11 31169 31168.50 31148.98 31166 31166.48 31146.96 12 31138 31138.48 31118.97 31136 31136.46 31116.95 13 31211 31210.59 31191.04 31209 31208.57 31189.02 14 31199 31198.53 31178.99 31197 31196.51 31176.97 15 31174 31174.47 31154.96 31172 31172.45 31152.94 16 31185 31185.49 31165.96 31183 31183.47 31163.94 17 31168 31167.56 31148.03 31166 31165.54 31146.01 18 31199 31198.53 31178.99 31197 31196.51 31176.97 19 31183 31182.53 31163.00 31181 31180.51 31160.98 20 31199 31198.53 31178.99 31197 31196.51 31176.97 21 31150 31150.49 31130.99 31148 31148.47 31128.97 22 31150 31150.47 31131.02 31148 31148.45 31129.00 23 31154 31154.48 31134.97 31152 31152.46 31132.95 24 31154 31154.42 31134.98 31152 31152.40 31132.96 25 31186 31186.48 31166.98 31184 31184.46 31164.96 26 31169 31168.50 31148.98 31166 31166.48 31146.96 27 31216 31215.52 31195.98 31214 31213.50 31193.96 28 31184 31183.52 31163.99 31182 31181.50 31161.97 29 31158 31158.47 31138.96 31156 31156.45 31136.94 30 31131 31131.46 31111.88 31129 31129.44 31109.86 31 31155 31154.52 31135.00 31153 31152.50 31132.98 32 31233 31233.48 31214.02 31231 31231.46 31212.00 33 31154 31153.54 31134.02 31152 31151.52 31132.00 34 31169 31169.49 31149.98 31167 31167.47 31147.96 35 31165 31164.50 31145.04 31162 31162.48 31143.02 36 31184 31183.56 31164.03 31182 31181.54 31162.01 37 31188 31188.49 31168.97 31186 31186.47 31166.95 38 31140 31139.51 31119.99 31137 31137.49 31117.97 39 31112 31112.44 31092.95 31110 31110.42 31090.93 40 31213 31212.56 31193.01 31211 31210.54 31190.99 41 31264 31264.45 31244.98 31262 31262.43 31242.96 42 31598 31597.98 31578.21 31596 31595.96 31576.19 43 31201 31200.50 31180.96 31198 31198.48 31178.94 Active protein Active protein (containing disulfide bond) Average Average molecular Monoisotopic molecular Monoisotopic Subtypes Da weight mass Da weight mass 1 29217 29217.14 29198.92 29215 29215.12 29196.90 2 29274 29274.19 29255.94 29272 29272.17 29253.92 3 29186 29186.11 29167.97 29184 29184.09 29165.95 4 29216 29216.14 29197.99 29214 29214.12 29195.97 5 29247 29247.17 29228.93 29245 29245.15 29226.91 6 29246 29246.23 29227.98 29244 29244.21 29225.96 7 29216 29216.20 29197.97 29214 29214.18 29195.95 8 29259 29259.22 29240.96 29257 29257.20 29238.94 9 29247 29247.17 29228.93 29245 29245.15 29226.91 10 29203 29203.12 29184.90 29201 29201.10 29182.88 11 29231 29231.17 29212.93 29229 29229.15 29210.91 12 29201 29201.14 29182.92 29199 29199.12 29180.90 13 29273 29273.25 29254.99 29271 29271.23 29252.97 14 29261 29261.20 29242.94 29259 29259.18 29240.92 15 29237 29237.13 29218.90 29235 29235.11 29216.88 16 29248 29248.15 29229.91 29246 29246.13 29227.89 17 29230 29230.23 29211.98 29228 29228.21 29209.96 18 29261 29261.20 29242.94 29259 29259.18 29240.92 19 29231 29231.17 29212.93 29229 29229.15 29210.91 20 29247 29247.17 29228.93 29245 29245.15 29226.91 21 29213 29213.15 29194.94 29211 29211.13 29192.92 22 29213 29213.14 29194.97 29211 29211.12 29192.95 23 29217 29217.14 29198.92 29215 29215.12 29196.90 24 29217 29217.08 29198.93 29215 29215.06 29196.91 25 29275 29275.18 29256.93 29273 29273.16 29254.91 26 29217 29217.14 29198.92 29215 29215.12 29196.90 27 29278 29278.18 29259.93 29276 29276.16 29257.91 28 29246 29246.18 29227.94 29244 29244.16 29225.92 29 29221 29221.13 29202.91 29219 29219.11 29200.89 30 29194 29194.12 29175.83 29192 29192.10 29173.81 31 29217 29217.19 29198.95 29215 29215.17 29196.93 32 29338 29338.23 29320.02 29336 29336.21 29318.00 33 29216 29216.20 29197.97 29214 29214.18 29195.95 34 29232 29232.16 29213.93 29230 29230.14 29211.91 35 29227 29227.16 29208.99 29225 29225.14 29206.97 36 29246 29246.23 29227.98 29244 29244.21 29225.96 37 29251 29251.16 29232.92 29249 29249.14 29230.90 38 29202 29202.17 29183.94 29200 29200.15 29181.92 39 29175 29175.10 29156.90 29173 29173.08 29154.88 40 29261 29261.20 29242.94 29259 29259.18 29240.92 41 29369 29369.20 29350.98 29367 29367.18 29348.96 42 29661 29660.65 29642.16 29659 29658.63 29640.14 43 29237 29237.13 29218.90 29235 29235.11 29216.88

    Example 8: Genotype Identification and Protein Identification of MBL Proteins and GES Derived from Clinical Strains

    [0166] In order to identify clinical strain-derived NDM, IMP and VIM (MBL proteins) and GES (Class A carbapenem protein), a strain confirmed as positive for CRE (Carbapenem-Resistant Enterobacteriaceae) was collected. The collected strain was genotyped for each gene using the PCR method.

    [0167] The strain whose genotype was confirmed was cultured using LB liquid medium, and whether MBL proteins (NDM, IMP and VIM) and GES protein were expressed was analyzed by SDS-PAGE gel analysis (FIGS. 2A, 2B, 2C, 2D and 2E).

    [0168] The clinical strain-derived MBL proteins and GES protein were digested by the in-gel digestion method and identified by the Bottom-Up method using the Q-Exactive HF-X plus system. It was confirmed that, for NDM, the sequence coverage for the identified peptides was 61.11% (165/270) in the full-length protein sequence and 65.74% (165/251) in the active form from which the N-terminus has been removed. It was confirmed that, for IMP and VIM, the sequence coverages for the identified peptides were 22.76% (56/246) and 44.74% (119/266), respectively, in the full-length protein sequences, and 24.56% (56/228) and 49.58% (119/240), respectively, in the active forms from which the N-terminus has been removed. In addition, it was confirmed that, for GES, the sequence coverage for the identified peptides were 67.25% (193/287) in the full-length amino acid sequence, and 71.75% (193/269) in the active protein sequence.

    [0169] Although the present invention has been described in detail with reference to the specific features, it will be apparent to those skilled in the art that this description is only of a preferred embodiment thereof, and does not limit the scope of the present invention. Thus, the substantial scope of the present invention will be defined by the appended claims and equivalents thereto.