NOVEL SPIRULINA PLATENSIS STRAIN

Abstract

The present invention relates to a novel spirulina strain having a linear shape, which spirulina strain can be used to achieve a harvest of spirulina strain with high yield.

Claims

1. An Arthrospira platensis NCB002 strain having a linear shape and deposited with accession number KCTC 13731BP.

2. The strain according to claim 1, the strain has a linear shape with a length of 0.5 to 3 cm.

3. The strain according to claim 1, wherein the strain is able to survive in a medium containing NH.sub.4Cl in an amount of 0.4 g/L or less.

4. The strain according to claim 1, wherein the strain can be harvested through a sieve having a mesh size of 0.1 to 1.0 mm.

5. A method for harvesting a spirulina strain, comprising the step of passing an Arthrospira platensis NCB002 strain having a linear shape and deposited with accession number KCTC 13731BP through a sieve having a mesh size of 0.1 to 1.0 mm.

6. The method according to claim 5, the strain has a linear shape with a length of 0.5 to 3 cm.

7. The method according to claim 5, wherein the strain is able to survive in a medium containing NH.sub.4Cl in an amount of 0.4 g/L or less.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0024] The above and other objects, features and advantages of the present invention will be more clearly understood from the following detailed description taken in conjunction with the accompanying drawings, in which:

[0025] FIG. 1 is a microscopic image of the novel spirulina strain culture;

[0026] FIG. 2 is a microscopic image of a known spiral-shaped spirulina strain culture; and

[0027] FIG. 3 shows the portions remaining after passing the novel spirulina strain culture (right-sided) and the known spiral-shaped spirulina strain culture (left-sided) through a sieve having a mesh size of 0.2 mm.

BEST MODE FOR CARRYING OUT THE INVENTION

[0028] Hereinafter, the present invention will be described in detail with reference to examples, comparative examples, and experimental examples, which are given for exemplary illustrations of the present invention and construed not to limit the scope of the present invention.

EXAMPLE 1

Isolation and Identification of Novel Spirulina Strain

[0029] The novel spirulina strain was obtained by mutating a known spirulina strain.

[0030] Specifically, a naturally occurring spirulina (Arthrospira platensis) strain was cultured in an SOT medium and then exposed to UV radiations. The morphology of the UV-exposed spirulina strain was observed with an optical microscope during cultivation to sort out the mutant strain of spirulina having a long linear shape unlike the common spirulina strains using a micromanipulator.

[0031] As a result, the sorted strain was named as Arthrospira platensis NCB002 strain and deposited under the accession number of KCTC 13731BP in the Korean Collection for Type Cultures (KCTC) on Nov. 22, 2018.

EXAMPLE 2

Cultivation of Strain

[0032] The novel spirulina strain isolated and identified in Example 1 was cultured in 100 mL of an SOT medium. The composition of the SOT medium was given as presented in the following Table 1, and that of a trace metal mix added to the SOT medium was as presented in the following Table 2.

TABLE-US-00001 TABLE 1 NaHCO.sub.3 16.8 g K.sub.2HPO.sub.4 0.5 g NaNO.sub.3 2.5 g K.sub.2SO.sub.4 1 g NaCl 1 g MgSO.sub.4•7H.sub.2O 0.2 g CaCl.sub.2•2H.sub.2O 0.04 g FeSO.sub.4•7H.sub.2O 0.01 g Na.sub.2EDTA•2H.sub.2O 0.08 g Trace metal mix A5* 1 ml Distilled water 1 L

TABLE-US-00002 TABLE 2 H.sub.3BO.sub.3 2.86 g MnCl.sub.2•4H.sub.2O 1.81 g ZnSO.sub.4•7H.sub.2O 0.22 g NaMoO.sub.4•2H.sub.2O 0.21 g CuSO.sub.4•5H.sub.2O 0.08 g CO(NO.sub.3).sub.2•6H.sub.2O 0.05 g Distilled water 1 L

[0033] On the other hand, the culture of the novel spirulina strain was observed for morphology with a microscope and its microscopic image was as shown in FIG. 1.

[0034] As can be seen from FIG. 1, the novel spirulina strain had a linear shape with a length of 1 cm or greater.

EXAMPLES 3 TO 6

Variation of NH4C1 Concentration of Culture Medium

[0035] The procedures were performed for cultivation of spirulina in the same manner as described in Example 2, excepting that the concentration of NH.sub.4Cl added to the medium for cultivation of spirulina was 0.1 g/L (Example 3), 0.2 g/L (Example 4), 0.3 g/L (Example 5), or 0.4 g/L (Example 6).

COMPARATIVE EXAMPLE 1

Variation of NH.SUB.4.Cl Concentration of Culture Medium

[0036] The procedures were performed for cultivation of spirulina in the same manner as described in Example 2, excepting that the concentration of NH.sub.4Cl added to the medium for cultivation of spirulina was 0.5 g/L (Comparative Example 1.

COMPARATIVE EXAMPLE 2

Cultivation of Spiral-Shaped Spirulina Strain

[0037] The known spiral-shaped spirulina strain was cultured in the same manner as described in Examples 1 and 2.

[0038] On the other hand, the culture of the known spiral-shaped spirulina strain was observed for morphology with a microscope and its microscopic image was as shown in FIG. 2.

COMPARATIVE EXAMPLES 3 TO 7

Variation of NH.SUB.4.Cl Concentration of Culture Medium

[0039] The procedures were performed for cultivation of the known spirulina strains in the same manner as described in Comparative Example 2, excepting that the concentration of NH.sub.4Cl added to the medium for cultivation of spirulina was 0.1 g/L (Comparative Example 3), 0.2 g/L (Comparative Example 4), 0.3 g/L (Comparative Example 5), or 0.4 g/L (Comparative Example 6), or 0.5 g/L (Comparative Example 7).

EXPERIMENTAL EXAMPLE 1

Resistance to NH.SUB.4.Cl

[0040] The novel spirulina strain or the spiral-shaped spirulina strain was cultured in a medium containing NH.sub.4Cl in the same manner as described in Examples 3 to 6 and Comparative Example 1, or

[0041] Comparative Examples 3 to 7 and visually inspected in regards to viability to determine the resistance of the novel spirulina strain to NH.sub.4Cl. In the following Table 3, “O” indicates the strain viable, and “X” indicates the strain inviable.

[0042] As can be seen from Table 3, the novel spirulina strain of the present invention was able to survive in a medium containing NH.sub.4Cl in an amount of 0.1 to 0.4 g/L, while the known spiral-shaped spirulina strain was unable to survive in a medium containing NH.sub.4Cl in an amount of more than 0.2 g/L.

TABLE-US-00003 TABLE 3 NH.sub.4Cl content (g/L) Strain type 0.1 0.2 0.3 0.4 0.5 Known spirulina strain ◯ ◯ X X X Novel spirulina strain ◯ ◯ ◯ ◯ X

EXPERIMENTAL EXAMPLE 2

Harvest of Strains

[0043] The novel spirulina strain of the present invention cultures in the same manner of Examples 1 and 2 and the known spirulina strain cultured in the manner of Comparative Example 2 were passed through a sieve having a mesh size of 0.2 mm for harvest.

[0044] The culture media of the novel spirulina strain and the known spirulina strain remaining after passing through the sieve having a mesh size of 0.2 mm were compared as shown in FIG. 3.

[0045] As can be seen from FIG. 3, the culture medium of the novel spirulina strain after passing through the sieve was clear (colorless) as the novel spirulina strain did not pass through the sieve having a mesh size of 0.2 mm, while the culture medium of the known spirulina strain was green as the known spirulina strain successfully passed through the sieve.

MODE FOR CARRYING OUT THE INVENTION

[0046] The present invention is directed to a novel spirulina strain having a long linear shape and a method for harvesting a spirulina strain using the novel spirulina strain.

INDUSTRIAL APPLICABILITY

[0047] The novel spirulina strain having a long linear shape and the method for harvesting a spirulina strain using the novel spirulina strain according to the present invention are applicable to the preparation of spirulina.