ANTI-HAIR LOSS LOTION

Abstract

It is provided a combination comprising diaminopyrimidine oxide, oleanolic acid, biotinoyl tripeptide-1, apigenin, biotin, adenosine or an adenosine derivative, and Gingko biloba, a topical composition comprising a therapeutically effective amount of the combination together with appropriate topical cosmetically or pharmaceutically acceptable excipients or carriers. It is also provided the composition for use in reducing or preventing the loss of hair and/or stimulating its growth.

Claims

1. A combination comprising diaminopyrimidine oxide, oleanolic acid, biotinoyl tripeptide-1, apigenin, biotin, adenosine or an adenosine derivative, and Gingko biloba.

2. A topical composition comprising a therapeutically effective amount of the combination of claim 1, together with appropriate topical cosmetically or pharmaceutically acceptable excipients or carriers.

3. The composition according to claim 2, wherein the weight ratio of the combination diaminopyrimidine oxide, oleanolic acid, biotinoyl tripeptide-1, apigenin, biotin, and adenosine or an adenosine derivative to Ginkgo biloba is from 60:1 to 15:1, particularly from 50:1 to 20:1.

4. The composition according to claim 1, which is a lotion wherein the carrier is a mixture of water and ethanol, the water being present in an amount from 25 wt. % to 70 wt. % of the total composition, and the ethanol being present in an amount from 75 wt. % to 30 wt. % of the total composition.

5. The composition according to claim 1, wherein the amount of diaminopyrimidine oxide is from 0.2 wt. % to 2 wt. %, particularly from 0.4 wt. % to 1.5 wt. %, of the total composition.

6. The composition according to claim 1, wherein the amount of oleanolic acid is from 0.00001 wt. % to 0.1 wt. %, particularly from 0.0001 wt. % to 0.01 wt. %, of the total composition.

7. The composition according to claim 1, wherein the amount of biotinoyl tripeptide-1 is from 0.00001 wt. % to 0.1 wt. % particularly from 0.0001 wt. % to 0.01 wt. % of the total composition.

8. The composition according to claim 1, wherein the amount of apigenin is from 0.0001 wt. % to 0.01 wt. %, particularly from 0.0005 wt. % to 0.01 wt. %, of the total composition.

9. The composition according to claim 1, wherein the amount of biotin is from 0.001 wt. % to 0.1 wt. %, particularly from 0.02 wt. % to 0.05 wt. %.

10. The composition according to claim 1, wherein the amount of adenosine or adenosine derivative is from 0.1 wt. % to 1 wt. %, particularly from 0.20 wt. % to 1 wt. %, the total composition.

11. The composition according to claim 10, which comprises an adenosine 5-phosphate derivative or a pharmaceutically acceptable salt thereof.

12. The composition according to claim 1, wherein the amount of Gingko biloba is from 0.001 wt. % to 0.1 wt. %, particularly from 0.01 wt. % to 0.08 wt. %.

13. The composition according to claim 1, further comprising finasteride.

14. The composition according to claim 1, further comprising minoxidil.

15. A method for reducing or preventing the loss of hair or stimulating its growth comprising administering a pharmaceutically effective amount of the composition as defined in claim 2 to a subject, particularly a man, in need of the treatment.

16. The composition according to claim 3, which is a lotion wherein the carrier is a mixture of water and ethanol, the water being present in an amount from 25 wt. % to 70 wt. % of the total composition, and the ethanol being present in an amount from 75 wt. % to 30 wt. % of the total composition.

17. The composition according to claim 16, wherein the amount of diaminopyrimidine oxide is from 0.2 wt. % to 2 wt. %, particularly from 0.4 wt. % to 1.5 wt. %, of the total composition.

18. The composition according to claim 17, wherein the amount of oleanolic acid is from 0.00001 wt. % to 0.1 wt. %, particularly from 0.0001 wt. % to 0.01 wt. %, of the total composition.

19. The composition according to claim 18, wherein the amount of biotinoyl tripeptide-1 is from 0.00001 wt. % to 0.1 wt. % particularly from 0.0001 wt. % to 0.01 wt. % of the total composition.

20. The composition according to claim 19, wherein the amount of apigenin is from 0.0001 wt. % to 0.01 wt. %, particularly from 0.0005 wt. % to 0.01 wt. %, of the total composition.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0020] All terms as used herein in this application, unless otherwise stated, shall be understood in their ordinary meaning as known in the art. Other more specific definitions terms as used in the present application are as set forth below and are intended to apply uniformly throughout the specification and claims unless an otherwise expressly set out definition provides a broader definition.

[0021] The term lotion, as used herein, the term lotion refers to any liquid preparation, particularly to a hydroalcoholic liquid formulation.

[0022] The term cosmetically acceptable as used herein, means that the compositions or components thereof so-described are suitable for use in contact with human skin without undue toxicity, incompatibility, instability, allergic response, or the like.

[0023] The expression pharmaceutically acceptable as used herein, means that the components so-described are compatible with the other ingredients of the pharmaceutical composition, and are suitable for use in contact with human skin without undue toxicity, incompatibility, instability, allergic response, or the like.

[0024] The expression therapeutically effective amount as used herein, refers to the amount of a compound that, when administered, is sufficient to prevent development of, or alleviate to some extent, one or more of the symptoms of the disorder or condition being treated. The particular dose of compound administered according to this invention will of course be determined by the particular circumstances surrounding the case, including the particular condition being treated, the duration of the treatment, the nature of any concurrent treatment, the specific combination of active ingredient used, and any other factor known to the expert.

[0025] The term percentage by weight or wt. % refers to the percentage by weight of the ingredient per weight of the overall composition.

[0026] As mentioned above, the combination of the invention comprises diaminopyrimidine oxide, oleanolic acid, biotinoyl tripeptide-1, apigenin, biotin, adenosine or an adenosine derivative, and Gingko biloba in the weight ratios mentioned above. It is particularly suitable for the preparation of formulations for topical application on the hair.

[0027] Weight ratios of the different components in the composition correspond to the weight ratios of the same components in the combination.

[0028] In a particular embodiment of the composition of the invention, optionally in combination with one or more features of the particular embodiments defined above, the weight ratio of the combination diaminopyrimidine oxide, oleanolic acid, biotinoyl tripeptide-1, apigenin, biotin and adenosine or an adenosine derivative to Ginkgo biloba is from 60:1 to 15:1, particularly from 50:1 to 20:1.

[0029] In another particular embodiment, the composition of the invention is a lotion wherein the carrier is a mixture of water and ethanol, the water being present in an amount from 25 wt. % to 70 wt. % of the total composition, and the ethanol being present in an amount from 75 wt. % to 30 wt. % of the total composition.

[0030] The use of the composition of the invention in the form of lotion comprising ethanol/water is specially advantageous since the specific combination of diaminopyrimidine oxide, oleanolic acid, biotinoyl tripeptide-1, apigenin, biotin, adenosine or an adenosine derivative, Gingko biloba, water and ethanol allows obtaining a stable anti-hair loss topical lotion providing a non-oily, non-sticky feeling when applied on the scalp. The liquid topical composition has a fluid, watery texture providing an intense non-greasy, non-sticky, fresh feeling. Additionally, the composition of the invention when is in the form of a lotion, slides soft and pleasant by massaging and is absorbed by the skin very quickly after its application, leaving a hair with a dry, clean, loose and flowing appearance. As a consequence, the product can be applied as many times as necessary, without the limitation of aesthetic concerns.

[0031] In another particular embodiment of the composition of the invention, optionally in combination with one or more features of the particular embodiments defined above, the amount of diaminopyrimidine oxide is from 0.2 wt. % to 2 wt. %, more particularly from 0.4 wt. % to 1.5 wt. %, of the total composition.

[0032] In another particular embodiment of the composition of the invention, optionally in combination with one or more features of the particular embodiments defined above, the amount of oleanolic acid is from 0.00001 wt. % to 0.1 wt. %, more particularly from 0.0001 wt. % to 0.01 wt. %, of the total composition.

[0033] In another particular embodiment of the composition of the invention, optionally in combination with one or more features of the particular embodiments defined above, the amount of biotinoyl tripeptide-1 is from 0.00001 wt. % to 0.1 wt. %, more particularly from 0.0001 wt. % to 0.01 wt, of the total composition.

[0034] In another particular embodiment of the composition of the invention, optionally in combination with one or more features of the particular embodiments defined above, the amount of apigenin is from 0.0001 wt. % to 0.01 wt. %, more particularly from 0.0005 wt. % to 0.01 wt. %, of the total composition.

[0035] In another particular embodiment, optionally in combination with one or more features of the particular embodiments defined above, the amount of biotin is from 0.001 wt. % to 0.1 wt. %, more particularly from 0.02 wt. % to 0.05 wt. %, more particularly from 0.001 wt. % to 0.05 wt. %, and even more particularly from 0.01 wt. % to 0.05 wt. %, of the total composition.

[0036] In another particular embodiment of the composition of the invention, optionally in combination with one or more features of the particular embodiments defined above, the amount of adenosine or adenosine derivative is from 0.1 wt. % to 1 wt. %, more particularly from 0.20 wt. % to 1 wt. %, of the total composition. In a particular embodiment, the composition of the invention comprises adenosine. In another particular embodiment, the composition of the invention comprises and adenosine 5-phosphate derivative or a pharmaceutically acceptable salt thereof, more particularly adenosine 5-triphosphate or a pharmaceutically acceptable salt thereof.

[0037] In another particular embodiment of the composition of the invention, optionally in combination with one or more features of the particular embodiments defined above, the amount of Gingko biloba is from 0.001 wt. %) to 0.1 wt. %, more particularly from 0.01 wt. % to 0.08 wt. %, and even more particularly from 0.01 wt. % to 0.1 wt. %, of the total composition.

[0038] In another particular embodiment of the composition of the invention, optionally in combination with one or more features of the particular embodiments defined above, the amount of diaminopyrimidine oxide is from 0.2 wt. % to 2 wt. % of the total composition, the amount of oleanolic acid is from 0.00001 wt. % to 0.1 wt. % of the total composition, the amount of biotinoyl tripeptide-1 is from 0.00001 wt. % to 0.1 wt. % of the total composition, the amount of apigenin is from 0.0001 wt. % to 0.01 wt. % of the total composition, the amount of biotin is from 0.02 wt. % to 0.05 wt. % of the total composition, the amount of adenosine or adenosine derivative is from 0.1 wt. % to 1 wt. % of the total composition, and the amount of Gingko biloba is from 0.001 wt. % to 0.1 wt. % of the total composition.

[0039] In another particular embodiment of the composition of the invention, optionally in combination with one or more features of the particular embodiments defined above, the amount of diaminopyrimidine oxide is from 0.4 wt. % to 1.5 wt. % of the total composition, the amount of oleanolic acid is from 0.0001 wt. % to 0.01 wt. % of the total composition, the amount of biotinoyl tripeptide-1 is from 0.0001 wt. % to 0.01 wt. % of the total composition, the amount of apigenin is from 0.0005 wt. % to 0.01 wt. % of the total composition, the amount of biotin is from 0.001 wt. % to 0.05 wt. % of the total composition, the amount of adenosine or adenosine derivative is from 0.20 wt. % to 1 wt. % of the total composition, and the amount of Gingko biloba is from 0.01 wt. % to 0.1 wt. % of the total composition.

[0040] As mentioned above, in the composition of the invention weight ratios of the different components are expressed with respect to the total amount of components of the composition. In the combination, the weight ratios of the components can also be expressed with respect to the total amount of components of the combination. Thus, the weight ratios of the different components with respect to the total amount of components of the composition correspond to the weight ratios of the same components in the combination with respect to the total amount of components of the combination.

[0041] As a way of example, weight percentages of the active ingredients for the composition mentioned above would correspond to the following weight percentages of the components in the combination of the invention, where excipients or carriers are not included: 64.44 wt. % to 55.97 wt. % of diaminopyrimidine oxide, 0.016 wt. % to 0.37 wt. % of oleanolic acid, 0.016 wt. % to 0.37 wt. % of biotinoyl tripeptide-1, 0.08 wt. % to 0.37 wt. % of apigenin, 1.61 wt. % to 1.87 wt. % of biotin, 32.22 wt. % to 37.31 wt. % of adenosine or adenosine derivative, and 1.61 wt. % to 3.73 wt. % of Gingko biloba, the total amount of components being 100%. Similarly, the weigh percentages of the components in the combinations used in the rest of specific compositions disclosed herein can be also calculated.

[0042] The compositions of the present invention can comprise or consist of, the components described herein. Accordingly, in a particular embodiment, the topical composition consists of diaminopyrimidine oxide, oleanolic acid, biotinoyl tripeptide-1, apigenin, biotin, adenosine or an adenosine derivative, and Gingko biloba, together with appropriate topical cosmetically or pharmaceutically acceptable excipients or carriers.

[0043] As mentioned above, the composition of the invention can comprise one or more appropriate topical cosmetically or pharmaceutically acceptable excipient such as antioxidants, preservatives, humectants, pH-regulating agents, chelating agents, among others. The appropriate cosmetically or pharmaceutically excipients and/or carriers and their amounts can readily be determined by those skilled in the art.

[0044] Examples of antioxidants include, without being limited to, tocopheryl acetate, tocopherol, butylated hydroxytoluene (BHT), ascorbyl palmitate, and vitamin C.

[0045] Examples of preservatives include, without being limited to, phenoxyethanol, methylparaben, propylparaben, sodium benzoate.

[0046] Examples of humectants include, without being limited to, PEG-6, PEG-8, PEG-32, glycerine, propylene glycol, butylene glycol, sodium hyaluronate, and hyaluronic acid.

[0047] Examples of pH-regulating agents include, without being limited to, sodium hydroxide, potassium hydroxide, triethanolamine, citric acid, and lactic acid.

[0048] Examples of chelating agents include, without being limited to, EDTA.

[0049] Additionally, the composition of the present invention may contain other ingredients, such as fragrances, colorants, and other components known in the state of the art for use in topical formulations.

[0050] The composition of the invention as defined above can be conceived as a base composition suitable for pharmaceutical compounding of ingredients commonly used in pharmaceutical compositions for use in reducing loss of hair. Accordingly, the composition of the present invention can contain additional active ingredients to those already mentioned. In a particular embodiment, optionally in combination with one or more features of the particular embodiments defined above, the composition of the invention further comprises finasteride, minoxidil, clobetasol, triamcinolone, clindamycin, ketoconazole, salicylic acid, or a combination thereof. Advantageously, any one of the mentioned additional active ingredients or a combination thereof can be incorporate in the composition of the invention without affecting to its stability, i.e. it is obtained a stable composition while maintaining the improved sensorial attributes of the base composition.

[0051] Finasteride is a drug known for the treatment of male androgenetic alopecia The drug, commercialized for oral administration, reduces loss of hair by inhibiting 5- reductase enzyme activity, which converts testosterone to its active form, namely di-hydrotestosterone (DHT), which is the main cause of male pattern hair loss. The physical chemical properties of Finasteride make difficult to obtain topical formulations with the sough effect on sensorial perception and appearance.

[0052] Minoxidil is a potassium channel opener, which applied topically is widely used for the treatment of hair loss. It is effective in helping promote hair growth in both males and females with androgenetic alopecia.

[0053] The mechanism by which minoxidil promotes hair growth is not fully understood. The poor solubility of minoxidil makes difficult to obtain topical formulations with good cosmeticity.

[0054] In a particular embodiment, optionally in combination with one or more features of the particular embodiments defined above, the composition of the invention further comprises finasteride, more particularly in an amount from 0.2 to 1.5, particularly of 0.5 wt. %. In another particular embodiment, optionally in combination with one or more features of the particular embodiments defined above, the composition of the invention further comprises minoxidil in an amount from 1 to 5, particularly of 3 wt. %.

[0055] The composition of the invention can be prepared by conventional methods known to those skilled in the art, such as the one described in the examples. An exemplary process may include the addition of diaminopyrimidine oxide, oleanolic acid, biotinoyl tripeptide-1, apigenin, biotin, adenosine or an adenosine derivative, and Gingko biloba into a mixture of water and ethanol with conventional mixing techniques until the solution is homogeneous.

[0056] Throughout the description and claims the word comprise and variations of the word, are not intended to exclude other technical features, additives, components, or steps. Furthermore, the word comprise encompasses the case of consisting of. The following examples are provided by way of illustration, and they are not intended to be limiting of the present invention. Furthermore, the present invention covers all possible combinations of particular and preferred embodiments described herein.

EXAMPLES

Example 1

[0057] This example illustrates an anti-hair loss lotion composition in accordance with the present invention.

TABLE-US-00001 Component wt. % Purified water q.s. Denatured alcohol 60.0 Diaminopyrimidine oxide 0.5 Gingko biloba 0.02 Oleanolic acid 0.001 Biotinoyl tripeptide-1 0.0001 PEG-40 Hydrogenated Castor oil 0.50 Apigenin 0.008 Adenosine 0.2 Biotin 0.03 Parfum (fragrance) 0.02 TOTAL 100.0000 q.s.: quantity sufficient to complete 100 g of topical composition

Manufacturing Method:

[0058] A lotion was prepared by the addition of diaminopyrimidine oxide, oleanolic acid, biotinoyl tripeptide-1, adenosine, and Gingko biloba into a mixture of water and ethanol with conventional mixing techniques until the solution is homogeneous. The rest of components are also added to the mixture until an homogeneous solution is obtained.

Cosmeticity

[0059] A study enrolling 20 men was carried out. The composition of Example 1 was applied with a slight massage onto the scalp of each one of the subjects. The cosmetic acceptability of the lotion was very good. After its application, the composition distributed uniformly without providing any oily, sticky feeling. The liquid topical composition had a fluid, watery texture and provided a non-greasy, non-sticky, fresh wet feeling. Additionally, the composition was quickly absorbed by the skin after its application, leaving a hair with a dry, clean, loose and flowing appearance. Furthermore, it was also stable.

Example 2

[0060] This example illustrates an anti-hair loss lotion composition in accordance with the present invention. The lotion was prepared as disclosed in Example 1.

TABLE-US-00002 Component wt. % Purified water q.s. Denatured alcohol 46 Diaminopyrimidine oxide 1.5 Gingko biloba 0.1 Oleanolic acid 0.0009 Biotinoyl tripeptide-1 0.0006 PEG-40 Hydrogenated Castor oil 1.00 Apigenin 0.0015 Adenosine 0.75 Biotin 0.01 Parfum (fragrance) 0.02 TOTAL 100.0000 q.s.: quantity sufficient to complete 100 g of topical composition

[0061] A similar cosmeticity as for lotion of Example 1 was observed.

Example 3

[0062] This example illustrates an anti-hair loss lotion composition in accordance with the present invention. The lotion was prepared as disclosed in Example 1.

TABLE-US-00003 Component wt. % Purified water q.s. Denatured alcohol 55.0 Diaminopyrimidine oxide 0.4 Gingko biloba 0.01 Oleanolic acid 0.0001 Biotinoyl tripeptide-1 0.0001 PEG-40 Hydrogenated Castor oil 0.50 Apigenin 0.005 Adenosine 0.1 Biotin 0.003 Parfum (fragrance) 0.02 TOTAL 100.0000 q.s.: quantity sufficient to complete 100 g of topical composition

[0063] A similar cosmeticity as for lotions of Example 1 and 2 was observed.

Example 4.Evaluation of Synergistic Effect of Ginkgo biloba on a Combination of Diaminopyrimidine Oxide, Oleanolic Acid, Biotinoyl Tripeptide-1, Apigenin, Biotin, and Adenosine

[0064] A study was conducted to evaluate the synergistic effect of the addition of Ginkgo biloba a hair lotion containing: diaminopyrimidine oxide, oleanolic acid, biotinoyl tripeptide-1, apigenin, biotin, and adenosine, in reducing hair loss in men with androgenetic alopecia.

Subjects and Methods

[0065] In a three-arm randomized study (1:1:1), subjects with type II and III androgenetic alopecia (AGA) were recruited; and were randomized to one of the following 3 groups: lotion with the sixactive agents diaminopyrimidine oxide, adenosine, oleanolic acid, biotinoyl tripeptide-1, apigenin and biotin (group A; as lotion of Example 2 without ginkgo biloba); lotion with ginkgo biloba (group B; as lotion of Example 2 without the rest of active agents); or lotion with the abovementioned six active agents+Ginko biloba (group C; lotion of Example 2). The lotion was applied on the scalp once daily for 6 months of treatment. The primary endpoint was the number of hairs in anagen and telogen phase by the use of TrichoScan through 3 and 6 months of treatment.

Results

[0066] In this study, 90 subjects with type II and III androgenetic alopecia (AGA) were included, of which 72 completed the study (24 subjects in group A, 23 in group B, and 25 in group C). The study showed a significant increase in the number of hairs in anagen phase after 3 and 6 months in the treatment groups A and C versus baseline, and at the same time a decrease in the number of hairs in telogen phase. Conversely, subjects in the treatment group B showed only a slight increase in the number of hairs in anagen phase and maintenance of hair in telogen phase during the study. Furthermore, the anagen/telogen ratio (NT) showed significant improvement in both treatment groups (A and C) versus baseline at 3 and 6 months. In particular, subjects of treatment group C showed a significant improvement compared to group A both at 3 and at 6 months (p<0.01).

TABLE-US-00004 basal A/T A/T 3 months A/T 6 months Group A (6 actives) 1.82 0.13 1.95 0.14 2.1 0.14 Group B (Gingko Biloba) 1.80 0.14 1.82 0.14 1.84 0.12 Group C (6 actives + 1.84 0.14 2.20 0.13 2.50 0.17 GB)

[0067] Surprisingly, it was also found that after discontinuing the treatment, the anagen/telogen ratio was significantly reduced to a lesser extent in group C compared to group A, and a significant increase in the number of hairs in the telogen phase in group A in relation to group C was observed. This was seen both at 3 and at 6 months after discontinuing the treatment.