ELECTROLYZED WATER COMPOSITION

Abstract

The present invention provides an aqueous electrolyte solution comprising at least four salts. The at least four salts are selected from: at least one salt selected from alkali metal chloride, alkali earth metal chloride, and ammonium chloride, or any combination thereof; at least one salt selected from alkali metal carbonate; alkali earth metal carbonate, and ammonium carbonate, or any combination thereof; at least one salt selected from alkali metal nitrate, alkali earth metal nitrate, and ammonium nitrate, or any combination thereof; and at least one salt selected from alkali metal phosphate, alkali earth metal phosphate, and ammonium phosphate, or any combination thereof.

Claims

1. An aqueous electrolyte solution comprising at least four salts, in which the at least four salts are selected from: at least one salt selected from alkali metal chloride, alkali earth metal chloride, and ammonium chloride, or any combination thereof; at least one salt selected from alkali metal carbonate; alkali earth metal carbonate, and ammonium carbonate, or any combination thereof; at least one salt selected from alkali metal nitrate, alkali earth metal nitrate, and ammonium nitrate, or any combination thereof; and at least one salt selected from alkali metal phosphate, alkali earth metal phosphate, and ammonium phosphate, or any combination thereof.

2. An aqueous electrolyte solution as claimed in claim 1, in which the electrolyte solution comprises: at least one alkali metal chloride salt and at least one alkali earth metal chloride salt; at least one alkali metal carbonate salt; at least one alkali metal nitrate salt and at least one alkali earth metal nitrate; and at least one alkali metal phosphate salt and/or at least one alkali earth metal phosphate salt.

3. An aqueous electrolyte solution as claimed in claim 1, in which the electrolyte solution comprises: at least one alkali metal chloride salt and at least one alkali earth metal chloride salt; and at least one alkali metal nitrate salt and at least one alkali earth metal nitrate salt.

4. An aqueous electrolyte solution as claimed in claim 3, in which the solution comprises: at least one alkali metal chloride salt; at least one alkali earth metal chloride salt; at least one salt selected from alkali metal nitrate; and at least one salt selected from alkali earth metal nitrate.

5. An aqueous electrolyte solution as claimed in claim 4, in which the solution comprises: sodium chloride (NaCl) and calcium chloride (CaCl.sub.2); magnesium nitrate (Mg(NO.sub.3).sub.2) and one or more alkali metal nitrate selected from: sodium nitrate (NaNO.sub.3) and potassium nitrate (KNO.sub.3), or any combination thereof.

6. A method for producing electrolyzed water composition for agricultural use or for ornamental preservation, the method comprising: preparing an aqueous electrolyte solution as claimed in claim 1; introducing the aqueous electrolyte solution into an electrolytic cell comprising a plurality of boron-doped diamond electrodes; and operating a power supply to apply a predetermined voltage to the electrolyte solution to produce an electrolyzed water biocidal composition comprising a plurality of active molecular and ionic species having biocidal activity, in which the salts of the electrolyte are selected such that the electrolyzed water composition comprises a free accessible chlorine (FAC) concentration in the range of from 10 to 10000 ppm, and/or a dissolved 03 concentration in the range of from 0.1 to 750 ppm.

7. A method as claimed in claim 6, in which the electrolyte solution is introduced into the electrolytic cell in a continuous or batch process manner.

8. A method as claimed in claim 6, in which the predetermined voltage is in the range of between 1 and 1000 volts DC.

9. A method as claimed in claim 6, in which the power supply has a current in the range of between 1 and 1000 ampere.

10. An apparatus for producing an electrolyzed water composition, the apparatus comprising: a reservoir comprising an aqueous electrolyte solution as claimed in claim 1; an electrolytic flow cell in fluid communication with the reservoir to receive a feed stream comprising the aqueous electrolyte solution; and a plurality of boron-doped diamond electrodes located within the electrolytic cell and arranged in use to be connected to a power supply.

11. An electrolyzed water composition obtainable by a method as claimed in claim 6.

12. Use of the electrolyzed water composition as claimed in claim 11 as a biocidal agent; or as a rehydration agent.

13. An applicator for applying an electrolyzed water composition, in which the applicator comprises a reservoir comprising an electrolyzed water composition as claimed in claim 11, and a nozzle in fluid communication with the reservoir.

Description

BRIEF DESCRIPTION OF FIGURES

[0133] Embodiments of the present invention will now be described, by way of example, with reference to the following figures:

[0134] FIGS. 1A and 1B are photographic images illustrating the fungal colony growth of Fusarium;

[0135] FIGS. 10 and 1D are photographic images illustrating the fungicidal effect with regards to Fusarium of being treated with a strong solution of an electrolyzed water composition of Example 1 and a weak solution of an electrolyzed water composition of Example 1;

[0136] FIG. 2 is a graphical representation comparing the effect of applying the composition of Example 1, and two other electrolyzed water compositions of the present invention, and known pesticide Revus to downy mildew infected lettuce;

[0137] FIG. 3 is a photographic image illustrating the rehydration effect of the electrolyzed water composition of Example 4 on cut flowers;

[0138] FIG. 4A is a photographic image illustrating the condition of roses rehydrated with varying solutions at a time period of two days post rehydration;

[0139] FIG. 4B is a photographic image illustrating the condition of roses rehydrated with varying solutions at a time period of four days post rehydration; and

[0140] FIG. 4C is a photographic image illustrating the condition of roses rehydrated with varying solutions at a time period of eight days post rehydration.

DETAILED DESCRIPTION

Example 1Electrolyzed Water Composition

[0141] An aqueous electrolyte solution comprising 0.05 g/l sodium chloride, 0.267 g/l calcium chloride, 0.267 g/l anhydrous sodium carbonate, 0.133 g/l magnesium nitrate, 0.133 g/l potassium nitrate and 0.15 g/l mono potassium phosphate in water was prepared. The electrolyte solution was stored within a reservoir chamber in fluid communication with an electrolytic cell.

[0142] A feed stream comprising the electrolyte solution was introduced into an electrolytic flow cell. The feed stream can optionally include one or more additional salts to enhance the biocidal properties of the resultant electrolyzed water composition.

[0143] The electrolytic cell is a non-membrane electrolytic cell. It is however to be understood that any suitable electrolytic cell may be used.

[0144] The electrolytic cell comprises a casing, a plurality of boron doped diamond electrodes (BDEs) located within the cell, and metal contact plates used for transmitting charge across the electrolyte solution.

[0145] The BDEs are sheet-like components and are provided in a stack of between 3 and 10 sheets. Each sheet is located at a fixed distance away from an adjacent sheet. The distance between adjacent sheets of BDEs provides a cell gap, which is preferably less than 5 mm, for example between approximately 2 and 3 mm. The BDEs are provided in a plastic frame. The BDEs transmit charge across the electrolyte solution, inducing a strong dipole and creating positively and negatively charged radicals on alternate surfaces of the diamonds.

[0146] The electrolyte solution may be introduced into the electrolytic cell in any suitable manner so as to produce electrolyzed water composition in a continuous process or in a batch process. In the continuous process, the electrolyte solution may be introduced at a suitable flow rate, such as for example at a flow rate in the range of from 0.1 to 100 l/min, for example in the range of from 3 to 5 l/min. In the batch process, the electrolyte solution may have a flow rate of approximately 16 l/min.

[0147] A power supply was operated to apply a voltage in the range of between 1 and 1,000 Volt D.C. and a current within the range of from 1-1,000 ampere to the electrolyte solution.

[0148] The over-potential provided between the electrodes shifts the equilibrium within the electrolyte solution such that a range of active species ions and molecules are produced and remain within the electrolyzed water for a significant amount of time. The term significant amount of time is used herein to refer to at least a week, preferably at least two weeks, for example at least a month. The combination of active molecular and ionic species together with the over-potential which supports the equilibrium confers a variable degree of biocidal activity to the electrolyzed water composition.

[0149] The electrolytic cell preferably comprises an outlet through which the electrolyzed water composition exits the cell. The resulting electrolyzed water composition comprises a range of active molecular and ionic species which have biocidal properties.

[0150] The active molecular and ionic species include dissolved ozone and free accessible chlorine (FAC). The electrolyzed water composition according to this embodiment comprises dissolved ozone at a level of approximately 2.5 ppm. The electrolyzed water composition according to this embodiment comprises free accessible chlorine (FAC) at a level of approximately 350 ppm.

[0151] Although the electrolyzed water composition of the present invention contains dissolved ozone at a level of approximately 2.5 ppm, it is to be understood that the electrolyzed water composition of the present invention may comprise any suitable level of dissolved ozone within the range of between 0.1 and 750 ppm. Although the electrolyzed water composition of the present invention contains and FAC at a level of approximately 350 ppm, it is to be understood that the electrolyzed water composition of the present invention may comprise any suitable level of FAC within the range of between 10 and 1,000 ppm.

[0152] It is also to be understood that the electrolyzed water composition may be varied by varying one or more of: the components of the electrolyte composition, the concentration of the components within the electrolyte composition, the degree of over-potential, the current applied, or any combination thereof. In this way the biocidal properties of the electrolyzed water biocidal composition may be tailored to suit different agricultural targets, such as for example crops, pathogens, delivery mechanism, and time points, or any combination thereof. For example, the biocidal properties of the electrolyzed water biocidal composition may be tailored in relation to when the composition is to be applied, such as for example during preparation of growing beds, during sowing and/or during growing seasons.

[0153] The system may further comprise one or more flow regulators arranged in use to adjust the flow of the electrolyte feed stream between the reservoir and the cell.

[0154] The system may further comprise a heater arranged in use to adjust the temperature of the flow of the electrolyte feed stream and/or the electrolyte solution within the cell.

[0155] The system may further comprise a control system arranged in use to control the flow rate of the electrolyte feed stream as required, such as for example by controlling the flow regulator(s).

[0156] The system may comprise a control system arranged in use to control the power supply to the electrodes.

[0157] The system may comprise a control system arranged in use to control the temperature of the electrolyte solution.

[0158] Control of the temperature of the electrolyte solution, the flow rate of the electrolyte solution feed stream, and the power supply to the electrodes may be provided by a single control system. Alternatively, these factors may be controlled by separate control systems.

Example 2Fusarium Control on Soil Samples

[0159] FIGS. 1A to 1D are photographic images of soil samples infected with Fusarium. FIGS. 1A and 1B are not treated with the composition of Example 1. FIGS. 1C and 1D are treated with a weak solution and a strong solution of the composition of Example 1 respectively.

[0160] FIGS. 1A and 1B show fungal colony growth as represented by the diffuse large white regions. The dishes of FIGS. 1C and 1D only show bacterial colony growth as represent by small discrete white regions. The electrolyzed water biocidal composition of Example 1 therefore has a preferential fungicidal effect. It can be seen from these figures that the compositions of the present invention can be used to kill fungicidal activity, such as Fusarium and/or other pathogens, while leaving viable bacterial colonies.

[0161] The compositions of the present invention may be applied to an agricultural area or crop(s) so as to provide a preferential fungicidal effect whilst leaving the existing structure of the soil bacteria reasonably intact and unaffected. The underlying soil bacteria are needed for decomposition and absorption of nutrients. The compositions of the present invention can be used to treat fungicidal infestations while helping and/or maintaining nutrient absorption at plant roots and therefore improving crop yield whilst not effecting the existing structure of the soil. The compositions of the present invention may be applied within a predetermined dosage range to an agricultural area or crop(s) so as to provide a preferential fungicidal effect whilst leaving the existing structure of the soil reasonably intact and unaffected.

Example 3Treatment of Downy Mildew Infected Lettuce

[0162] Downy mildew infected lettuce was treated with five different treatments. Treatment 1: untreated control; Treatment 2 (comparative example): aqueous salt mix composition comprising sodium chloride and sodium carbonate in a ratio by weight of 7:8; Treatment 3: electrolyzed water composition of Example 1; Treatment 4 (comparative example): aqueous salt mix composition comprising sodium chloride and calcium chloride in a ratio by weight of 50:50; and Treatment 5: Revus (known pesticide).

[0163] The treatments were applied using a foliar spray. Each treatment group consisted of four replicate plots each containing 8 plants. Each treatment was sprayed onto the plots for 2 minutes. Each treatment was applied weekly for three weeks. Data was collected immediately on harvest. Harvest occurred 3 days after the last application of a treatment. FIG. 2 illustrates the degree of crop infection as represented by the percentage of Downy Mildew remaining on the lettuce after treatment. It can be seen that the composition of the present invention (Treatment 3) provides a pesticidal effect and significantly reduce the percentage of Downy Mildew when compared with the untreated control. Treatment 3 performs at least as well as the known pesticide (Treatment 5).

[0164] The method of biocidal treatment of a substrate using the composition of the present invention for soil sterilisation has significantly reduced environmental issues compared to conventional methods. In contrast to a number of conventional soil sterilisation methods, the present invention does not require a considerable diesel supply to heat the surface soil, and does not require a large water supply to produce a large volume of steam. Furthermore, the compositions of the present invention provide significant biocidal activity whilst only containing salts which are already present within fertiliser and plant micronutrients. The compositions of the present invention are therefore more environmentally friendly than known biocidal compositions. Furthermore, the compositions of the present invention do not leave any harmful chemical residues on treated food. As such, the compositions of the present invention may be used more frequently, during extended periods of crop production, such as for example closer to crop harvest, and without requiring any additional health and safety protection or equipment.

[0165] It is to be understood that the Examples are illustrative of the biocidal properties of the compositions of the present invention. It is to be understood that the compositions of the present invention may be applied in any suitable manner to an agricultural area or crop(s).

[0166] Although the above Examples illustrate the use of the compositions of the present invention for the treatment of soil and crops, it is to be understood that the compositions of the present invention may be used in any industry, in particular the agricultural industry, which requires the use of biocidal compositions. For example, the compositions of the present invention may be used to treat any equipment, such as for example irrigation systems, tanks including water tanks, and/or crop treatment equipment as well as water such as for example surface, rain and/or ground water.

Example 4Electrolyzed Water Composition

[0167] An aqueous electrolyte solution comprising 0.3 g/l sodium chloride, 1.6 g/l calcium chloride, 1.3 g/l magnesium nitrate, and 2.8 g/l potassium nitrate in water was prepared. The electrolyte solution was stored within a reservoir chamber in fluid communication with an electrolytic cell.

[0168] A feed stream comprising the electrolyte solution was introduced into an electrolytic flow cell. The feed stream can optionally include one or more additional salts to enhance the rehydration properties of the resultant electrolyzed water composition.

[0169] The electrolytic cell is a non-membrane electrolytic cell. It is however to be understood that any suitable electrolytic cell may be used.

[0170] The electrolytic cell comprises a casing, a plurality of boron doped diamond electrodes (BDEs) located within the cell, and metal contact plates used for transmitting charge across the electrolyte solution.

[0171] The BDEs are sheet-like components and are provided in a stack of between 3 and 10 sheets. Each sheet is located at a fixed distance away from an adjacent sheet.

[0172] The distance between adjacent sheets of BDEs provides a cell gap, which is preferably less than 5 mm, for example between approximately 2 and 3 mm. The BDEs are provided in a plastic frame. The BDEs transmit charge across the electrolyte solution, inducing a strong dipole and creating positively and negatively charged radicals on alternate surfaces of the diamonds.

[0173] The electrolyte solution may be introduced into the electrolytic cell in any suitable manner so as to produce electrolyzed water composition in a continuous process or in a batch process. In the continuous process, the electrolyte solution may be introduced at a suitable flow rate, such as for example at a flow rate in the range of from 0.1 to 100 l/min, for example in the range of from 3 to 5 l/min. In the batch process, the electrolyte solution may have a flow rate of approximately 16 l/min.

[0174] A power supply was operated to apply a voltage in the range of between 1 and 1,000 Volt D.C. and a current within the range of from 1-1,000 ampere to the electrolyte solution.

[0175] The over-potential provided between the electrodes shifts the equilibrium within the electrolyte solution such that a range of active species ions and molecules are produced and remain within the electrolyzed water for a significant amount of time. The term significant amount of time is used herein to refer to at least a week, preferably at least two weeks, for example at least a month. The combination of active molecular and ionic species together with the over-potential which supports the equilibrium confers a variable degree of rehydration activity to the electrolyzed water composition.

[0176] The electrolytic cell preferably comprises an outlet through which the electrolyzed water composition exits the cell. The resulting electrolyzed water composition comprises a range of active molecular and ionic species which have enhanced rehydration properties.

[0177] The active molecular and ionic species include dissolved ozone and free accessible chlorine (FAC). The electrolyzed water composition according to this embodiment comprises dissolved ozone at a level of approximately 2.5 ppm. The electrolyzed water composition according to this embodiment comprises free accessible chlorine (FAC) at a level of approximately 250 ppm.

[0178] Although the electrolyzed water composition of the present invention contains dissolved ozone at a level of approximately 2.5 ppm, it is to be understood that the electrolyzed water composition of the present invention may comprise any suitable level of dissolved ozone within the range of between 0.1 and 750 ppm. Although the electrolyzed water composition of the present invention contains and FAC at a level of approximately 250 ppm, it is to be understood that the electrolyzed water composition of the present invention may comprise any suitable level of FAC within the range of between 10 and 1,000 ppm.

[0179] It is also to be understood that the electrolyzed water composition may be varied by varying one or more of: the components of the electrolyte composition, the concentration of the components within the electrolyte composition, the degree of over-potential, the current applied, or any combination thereof. In this way the rehydration properties of the electrolyzed water composition may be tailored to suit different ornamental targets, such as for example flower species, microbial contaminants, delivery mechanisms, and growing environment, or any combination thereof. For example, the rehydration properties of the electrolyzed water composition may be tailored in relation to when the composition is to be applied.

[0180] The system may further comprise one or more flow regulators arranged in use to adjust the flow of the electrolyte feed stream between the reservoir and the cell.

[0181] The system may further comprise a heater arranged in use to adjust the temperature of the flow of the electrolyte feed stream and/or the electrolyte solution within the cell.

[0182] The system may further comprise a control system arranged in use to control the flow rate of the electrolyte feed stream as required, such as for example by controlling the flow regulator(s).

[0183] The system may comprise a control system arranged in use to control the power supply to the electrodes.

[0184] The system may comprise a control system arranged in use to control the temperature of the electrolyte solution.

[0185] Control of the temperature of the electrolyte solution, the flow rate of the electrolyte solution feed stream, and the power supply to the electrodes may be provided by a single control system. Alternatively, these factors may be controlled by separate control systems.

Example 5Rehydration of Cut Flowers

[0186] With reference to FIG. 3, an experiment was carried out to determine the effect on flowers of being stored in three different rehydration solutions. The flowers were placed in three pairs of buckets. Each pair of buckets contained a different rehydration solution for cut flowers. A first pair of buckets 1A, 1B contained water; a second pair of buckets 2A, 2B contained a conventional rehydration solution (Chrysal Professional T-bag); and a third pair of buckets 3A, 3B contained the Electrolysed Water Composition of Example 4 (5 g/l total salts, 250 ppm FAC). A first bucket from each pair of bucket contained gerboras and a second bucket from each pair of buckets contained roses.

[0187] The stems of the cut flowers (in this case roses and gerboras) were cut to remove about 2 cm in length. The cut flowers are placed within the buckets containing the corresponding rehydration solution. The flowers remained in the solution for five days at ambient temperature. The results are shown in FIG. 3.

[0188] As shown in FIG. 3, the flowers in the third pair of buckets 3A, 3B are observed to be in a much healthier condition than the flowers placed in either water or in conventional rehydration solution.

[0189] The present invention therefore provides compositions with improved rehydration properties for cut flowers compared to conventional rehydration solutions and water.

Example 6Method for Handling Dry Roses

Rehydration of Roses:

[0190] 30 bunches of dry roses (10 pink roses, 10 white roses and 10 yellow roses) arrive dry in boxes after import by air from Kenya. The roses are removed from the boxes. 2 cm of length is cut from the base of the stem. Roses are then placed in the corresponding rehydration solution (either a) water or b) the composition of Example 1) with cardboard collars in place. The roses remain in the rehydration solution overnight (approx. 9 hours) at ambient temperature. The rehydrated roses are then removed from the rehydration solution and shipped as bunches. The cardboard collars are discarded at this stage.

Shop Floor

[0191] A further 1 cm is removed from the base of the rehydrated stem. The roses were placed in one of ten buckets (two sets of five buckets). A first set of five buckets (marked B) received the roses which have previously been rehydrated in water, and the other set of five buckets (marked A) received the roses which have previously been rehydrated with the composition of Example 4.

[0192] The roses were placed at room temperature (approx. 20 C.) for four days and time lapse photography is initiated (FIGS. 4A and 4B).

[0193] Each set of five buckets comprises 2 litres of one of five different solutions (Ship to Store liquids as shown in Table 1):

1) water 10 A/B;
2) water and proff 2 Crysal T-bag 12 A/B;
3) dilute composition of S1 (1.0 g/l NaCl solution) 14 A/B;
4) dilute composition of Example 4 16 A/B;
5) dilute Composition of Example 4 variant with alternative formulation as shown in Table 1 18 A/B.

TABLE-US-00001 TABLE 1 Composition Composition of of Example 4 Water T-bag Dilute S1 Example 4 variant 10 A/B 12 A/B 14 A/B 16 A/B 18 A/B ORP 305 275 745 490 668 FAC (ppm) 0.31 0.11 147.8 48.4 22.8

[0194] The term ORP is used herein to refer to the oxidative reduction potential. The oxidative reduction potential is a measure of the amount of antimicrobial efficacy that an electrolysed water solution contains.

[0195] As shown in FIGS. 4A and 4B show the condition of the rehydrated (either with water or with the composition of Example 4) roses placed in varying solutions. The roses performed well over the period of four days post-rehydration (FIG. 4B). The rehydrated roses placed in water (buckets 10 A/B) appear to begin to droop after four days post-rehydration. A few of the roses placed within the water (buckets 10A/B) are also failing to open and a number of petals shrivelled after a period of four days post rehydration treatment.

Customer Vase

[0196] The roses are then transferred to cleaned buckets. The consumer sachet instructions for caring for the roses were followed accordingly for 8 days and again time lapse photography was initiated. The number of wilted flowers were counted for each colour rose within each vase on Day 1 (Table 2), Day 3 (Table 3) and Day 8 (Table 4).

Day 1:

[0197]

TABLE-US-00002 TABLE 2 Ship to Store liquid Rehydration Composition Composition of Composition of Example 4 Example 4 Water T bag 10% S1 of Example 4 Variant Yellow Roses 0/10 1/10 wilted 0/10 wilted 0/10 wilted 0/10 wilted wilted White Roses 2/6 wilted 0/8 wilted 0/9 wilted 0/9 wilted 0/9 wilted 1 N/O Pink Roses 0/10 0/10 wilted 0/10 wilted 0/10 wilted 0/10 wilted wilted Composition Composition of Example 1 Water Water T bag 10% S1 of Example 1 Variant Yellow Roses 0/10 0/10 wilted 0/10 wilted 0/10 wilted 0/10 wilted wilted White Roses 1/10 0/10 wilted 3/10 wilted.sup.1 0/10 wilted 0/10 wilted wilted 3 N/O Pink Roses 0/10 0/10 wilted 0/10 wilted 0/10 wilted 0/10 wilted wilted

Day 3

[0198]

TABLE-US-00003 TABLE 3 Ship to Store liquid Rehydration Composition Composition of Composition of Example 4 Example 4 Water T bag 10% S1 of Example 4 Variant Yellow Roses 10/10 4/10 wilted 2/10 wilted 0/10 wilted 0/10 wilted wilted White Roses 4/6 wilted 1/8 wilted 0/9 wilted 0/9 wilted 0/9 wilted Pink Roses 10/10 1/10 wilted 0/10 wilted 0/10 wilted 1/10 wilted wilted Composition Composition of Example 1 Water Water T bag 10% S1 of Example 1 Variant Yellow Roses 7/10 wilted 7/10 wilted 0/10 wilted 1/10 wilted 2/10 wilted White Roses 10/10 5/10 wilted 1/10 wilted 0/10 wilted 1/10 wilted wilted 3 N/O Pink Roses 10/10 8/10 wilted 1/10 wilted 0/10 wilted 2/10 wilted wilted

Day 8

[0199]

TABLE-US-00004 TABLE 4 Ship to Store liquid Rehydration Composition Composition Composition of Example 4 of Example 4 Water T bag 10% S1 of Example 4 Variant Yellow Roses 10/10 wilted 6/10 wilted 5/10 wilted 2/10 wilted 6/10 wilted White Roses 3/6 wilted 2/8 wilted 4/9 wilted 0/9 wilted 1/9 wilted Pink Roses 9/10 wilted 4/10 wilted 0/10 wilted 4/10 wilted 4/10 wilted 1 N/O Composition Composition of Example 1 Water Water T bag 10% S1 of Example 1 Variant Yellow Roses 10/10 wilted 6/10 wilted 0/10 wilted 4/10 wilted 6/10 wilted all with all with shrivelled shrivelled petals petals White Roses 10/10 wilted 5/10 wilted 8/10 wilted 1/10 wilted 3/10 wilted 3 N/O Pink Roses 10/10 wilted 4/10 wilted 1/10 wilted 5/10 wilted 10/10 wilted all with shrivelled petals

[0200] It was found that roses wilting/drooping was the primary failure, with some roses not opening (N/O). As the blooms aged, the petals become shrivelled or slightly dessicated.

[0201] In total, the flower loss after rehydration, 4 days of simulated retail environment, and 8 days of simulated home display was 93% ( 52/56) for roses rehydrated in water; 48% ( 28/58) for roses in Chrysal T-Bag solution, and just 27% ( 16/59) for those stored in the composition of Example 4.

[0202] It can therefore be seen that the compositions of the present invention significantly improve the shelf life of cut flowers. The improvement in shelf life obtained by using the compositions of the present invention to rehydrate cut flowers is equivalent to increasing the shelf life of the cut flowers by 2-3 days. It is to be noted that although the present invention provides results for roses, that the compositions of the present invention have a similar effect on other cut flowers. The present invention therefore provides compositions which can be used to increase the shelf life of flowers, to increase the quality of the cut flowers sold to consumers, to increase the effective yield, and thereby reducing the number of guarantee claims brought by customers.

[0203] It is to be understood that Examples 4 to 6 are illustrative of the rehydration properties of the compositions of the present invention for roses. It is to be understood that the compositions of the present invention may be applied in any suitable manner to any species of ornamental crop(s) or pathogen.