PIGLET FEED BASED ON BACTERIAL ENZYME SYNERGISTIC FERMENTATION PROCESS AND PREPARATION METHOD THEREOF

Abstract

Disclosed is a piglet feed based on bacteria enzyme synergistic fermentation process. The piglet feed is composed of basic components and bacteria enzyme synergistic fermentation feed. Basic components include soybean protein concentrate, whey powder, fish meal, sodium chloride, choline chloride, stone powder, calcium hydrogen phosphate, composite vitamins, composite trace elements, and composite amino acids. The bacterial enzyme synergistic fermentation feed includes a fermentation substrate, an enzyme preparation, and a bacterial strain. The bacterial enzyme synergistic fermentation feed can not only improve the production performance of piglets, but also improve the utilization rate of feed nutrients, especially the utilization rate of feed phosphorus, thereby reducing the excretion of phosphorus in feces.

Claims

1. A piglet feed based on bacterial enzyme synergistic fermentation process, wherein the piglet feed consists of 10-20% by weight of basic components and 80-90% by weight of bacterial enzyme synergistic fermentation feed; a total of 100% by weight of the basic component comprises 26.67% by weight of soy protein concentrate, 26.67% by weight of whey powder, 26.67% by weight of fish meal, 2.67% by weight of sodium chloride, 0.8% by weight of choline chloride, 6% by weight of stone powder, 3.46% by weight of hydrogen phosphate calcium, 0.33% by weight of compound vitamins, 1.33% by weight of compound trace elements, and 5.4% by weight of compound amino acids; the bacterial enzyme synergistic fermentation feed comprises a fermentation substrate, an enzyme preparation, and a bacterial strain; and a total of 100% by weight of the fermentation substrate comprises 36% by weight of corn, 36% by weight of expanded corn, 12% by weight of soybean meal, 12% by weight of expanded soybean, 2% by weight of sucrose, and 2% by weight of soybean oil.

2. The piglet feed according to claim 1, wherein in the bacterial enzyme synergistic fermentation feed, the total concentration of the bacterial strain is 1-2×10.sup.8 (CFU/mL).

3. The piglet feed according to claim 1, wherein the enzyme preparation is composed of 0.03-0.06% by weight of protease, 0.03-0.06% by weight of amylase, 0.02-0.04% by weight of lipase, and 0.02-0.04% by weight of cellulase.

4. The piglet feed according to claim 1, wherein the bacterial strain consists of Lactobacillus plantarum (CGMCC1.12934), Pediococcus pentosaceus (CGMCC1.12961), and Aspergillus niger (CGMCC3.4304), and each of which is inoculated with 1-2 mL.

5. The piglet feed according to claim 1, wherein the bacterial strain consists of 1-2 mL of Lactobacillus plantarum (CGMCC1.12934), 1-2 mL of Pediococcus pentosaceus (CGMCC1.12961), and 1-2 mL of Aspergillus niger (CGMCC3.4304).

6. The piglet feed according to claim 1, wherein the multivitamin comprises vitamin A, vitamin D3, vitamin E, vitamin K3, vitamin B1, vitamin B2, vitamin B6, vitamin B12, calcium pantothenate, folic acid, biotin, niacin, and vitamin C.

7. The piglet feed according to claim 1, wherein the composite trace element comprises copper sulfate, ferrous sulfate, manganese sulfate, calcium iodate, and ultrafine sodium selenite.

8. The piglet feed according to claim 1, wherein the composite amino acid comprises lysine, methionine, threonine, and tryptophan.

9. A preparation method of the piglet feed of claim 1, comprising the following steps: providing Lactobacillus plantarum (CGMCC1.12934), Pediococcus pentosaceus (CGMCC1.12961), Aspergillus niger (CGMCC3.4304); providing protease (>50 000 U/g), amylase (>7 000 U/g), lipase (>8 000 U/g), cellulase (>10 000 U/g) and mixing them well; mixing the fermentation substrate uniformly in proportions, adding water according to the ratio of 1:0.5, adding the enzyme preparation and the bacterial strain in sequence during a stirring process, and fermenting at 28-30° C. for 12 h; and mixing the basic component uniformly in proportions, adding the bacterial enzyme synergistic fermentation feed prepared in step (3), and stirring the basic component and the bacterial enzyme synergistic fermentation feed uniformly.

Description

DETAILED DESCRIPTION OF THE DISCLOSURE

[0026] A piglet feed based on bacterial enzyme synergistic fermentation process is provided. The piglet feed is consisted of 15% basic components and 85% bacterial enzyme synergistic fermentation feed. All percentages expressed on a weight basis.

[0027] A total of 100% of the basic component comprises 26.67% soy protein concentrate, 26.67% whey powder, 26.67% fish meal, 2.67% sodium chloride, 0.8% choline chloride, 6% stone powder, 3.46% hydrogen phosphate calcium, 0.33% compound vitamins, 1.33% compound trace elements, 5.4% compound amino acids. All percentages expressed on a weight basis.

[0028] The bacterial enzyme synergistic fermentation feed comprises a fermentation substrate, an enzyme preparation, and a bacterial strain.

[0029] A total of 100% of the fermentation substrate comprises 36% corn, 36% expanded corn, 12% soybean meal, 12% expanded soybean, 2% sucrose, and 2% soybean oil. All percentages expressed on a weight basis.

[0030] In the bacterial enzyme synergistic fermentation feed, the total concentration of the bacterial strain is 2×10.sup.8 (CFU/mL). Based on the fermentation substrate, the enzyme preparation is added by 0.2%. The bacterial strain is composed of Lactobacillus plantarum (CGMCC1.12934), Pediococcus pentosaceus (CGMCC1.12961), and Aspergillus niger (CGMCC3.4304). Preferably, the bacterial strain consists of 1-2 mL of Lactobacillus plantarum (CGMCC1.12934), 1-2 mL of Pediococcus pentosaceus (CGMCC1.12961), and 1-2 mL of Aspergillus niger (CGMCC3.4304). The enzyme preparation is composed of 0.06% protease, 0.05% amylase, 0.04% lipase, and 0.02% cellulase.

[0031] The multivitamin comprises vitamin A, vitamin D3, vitamin E, vitamin K3, vitamin B1, vitamin B2, vitamin B6, vitamin B12, calcium pantothenate, folic acid, biotin, niacin, and vitamin C. There is no specific amount of each component, and generally meet the needs of animals. The following trace elements and compound amino acids are also the same.

[0032] The composite trace element comprises copper sulfate, ferrous sulfate, manganese sulfate, calcium iodate, and ultrafine sodium selenite. The composite amino acid comprises lysine, methionine, threonine, and tryptophan.

[0033] The preparation method of the piglet feed comprises the following steps:

[0034] (1) providing Lactobacillus plantarum (CGMCC1.12934), Pediococcus pentosaceus (CGMCC1.12961), Aspergillus niger (CGMCC3.4304);

[0035] (2) providing protease (>50 000 U/g), amylase (>7 000 U/g), lipase (>8 000 U/g), cellulase (>10 000 U/g) and mixing them well;

[0036] (3) mixing the fermentation substrate uniformly in proportions, adding water according to the ratio of 1:0.5, adding the enzyme preparation and the bacterial strain in sequence during a stirring process, and fermenting at 28-30° C. for 12 h; and

[0037] (4) mixing the basic component uniformly in proportions, adding the bacterial enzyme synergistic fermentation feed prepared in step (3), and mixing them uniformly.

[0038] The present disclosure has carried out a systematic study on the feed prepared in the examples. The 21-day-old weaned piglet was used as a test animal, and the piglet was fed an unfermented and fermented diet composed of the same raw material to investigate the growth performance, nutrient digestibility and utilization rate of the jejunal digestive enzyme activity.

Example 1

[0039] The 21-day-old weaned piglets were used as experimental animals. The method was divided into 2 groups using a randomized block design method, each with 8 replicates and 3 piglets in each replicate. The control group was fed unfermented piglet feed and the test group was fed fermented feed. The test period is 49 days. The average daily feed intake, daily gain, and diarrhea index of piglets are investigated. The results are shown in Table 1. The results showed that compared with the control group, the test group significantly increased the piglet body weight and average daily gain, and significantly reduced the piglet's diarrhea.

TABLE-US-00001 TABLE 1 Effect on the Production Performance and Diarrhea Rate of Weaned Piglets Item Control Test SEM Value P Initial Weight (kg) 7.16 7.16 0.30 1.00 Fina Weight (kg) 25.64 28.63 0.50 0.05 Average Daily Gain (g) 376 437 10 0.03 Average Daily Feed Intake (g) 650 705 14 0.22 Feed Conversion Ratio 1.73 1.62 0.03 0.27 Diarrhea Index 0.12 0.04 0.02 0.03

Example 2

[0040] The 21-day-old weaned piglets were used as experimental animals. The method was divided into 2 groups using a randomized block design method, each with 8 replicates and 3 piglets in each replicate. The control group was fed unfermented piglet feed, and the test group was fed fermented feed. The test period is 49 days. The nutrient digestibility of piglets is investigated. The results are shown in Table 2. The results showed that the test group significantly improved the apparent digestibility of dietary dry matter, crude protein, crude fat and phosphorus, and the utilization of phosphorus increased by 52%.

TABLE-US-00002 TABLE 2 Effect on the Apparent Digestibility of Nutrients in Weaned Piglets (%) Item Control Test SEM Value P Dry Material (DM) 77 84 1 <0.01 Crude Protein (CP) 67 76 1 <0.01 Ether Extract (EE) 53 67 2 <0.01 Ash 44 57 2 <0.01 Total Phosphorus (TP) 25 38 2 0.03

Example 3

[0041] The 21-day-old weaned piglets were used as experimental animals, and they were divided into 2 groups using a random block design method, each with 8 replicates, and each replicate with 3 piglets. The control group was fed unfermented piglet feed, and the test group was fed fermented feed. The test period was 49 days. The digestive enzyme activity of the piglet's jejunum is investigated. The results are shown in Table 3.

TABLE-US-00003 TABLE 3 Digestive Enzyme Activity Item Control Test SEM Value P Sucrase (U/mg prot) 110 168 8 0.03 Maltase (U/mg prot) 117 115 9 0.77 Amylase (U/mg prot) 1.26 1.62 0.05 <0.01

[0042] The results showed that the test group significantly improved the activities of sucrase and amylase in the jejunum mucosa of piglets by 53% and 29%, respectively.