INSECT AND ACARINA PEST CONTROL

Abstract

The present invention relates to a method of controlling or preventing damage to a plant, which comprises applying on the plant, the locus thereof or its propagation material a combination comprising, as component (A), a compound of formula (I) or an agrochemically acceptable salt thereof; and, as component (B), a compound selected from a group consisting of mixing partners; and to compositions, formulations and concentrates comprising such combinations.

##STR00001##

Claims

1. A method of controlling or preventing damage to a plant, which comprises applying on the plant, the locus thereof or its propagation material a combination comprising, as component (A), a compound of formula (I): ##STR00007## or an agrochemically acceptable salt thereof, and, as component (B), a compound selected from the group consisting of: B-1 nicofluprole (CAS 1771741-86-6); B-2 spidoxamat (CAS 907187-07-9); B-3 N-[[2-fluoro-4-[2-hydroxy-3-(3,4,5-trichlorophenyl)-3-(trifluoromethyl)pyrrolidin-1-yl]phenyl]methyl]cyclopropanecarboxamide (CAS 1365070-72-9); B-3a (S)N-[[2-fluoro-4-[2-hydroxy-3-(3,4,5-trichlorophenyl)-3-(trifluoromethyl)pyrrolidin-1-yl]phenyl]methyl]cyclopropanecarboxamide (CAS 2044701-44-0); B-4 4-dimethyl-2-[2-(3-pyridyl)indazol-5-yl]-1,2,4-triazolidine-3,5-dione (CAS 2171099-09-3); B-5 N-[3-chloro-1-(3-pyridyl)pyrazol-4-yl]-2-methylsulfonyl-propanamide (CAS 2396747-83-2); B-6 (3R)-3-(2-chlorothiazol-5-yl)-8-methyl-5-oxo-6-phenyl-2,3-dihydrothiazolo[3,2-a]pyrimidin-8-ium-7-olate (CAS 2246757-58-2); B-7 1-(2-cyanoethyl)-4-oxo-3-[3-(trifluoromethyl)phenyl]pyrido[1,2-a]pyrimidin-1-ium-2-olate (CAS 2032403-97-5); B-8 N-isopropyl-2-(3-pyridyl)indazole-4-carboxamide (CAS 1680187-98-7); B-9 N-cyclopropyl-2-(3-pyridyl)indazole-4-carboxamide (CAS 1680188-04-8); B-10 N-cyclohexyl-2-(3-pyridyl)indazole-4-carboxamide (CAS 1680188-06-0); B-11 2-(3-pyridyl)-N-(2,2,2-trifluoroethyl)indazole-4-carboxamide (CAS 1680188-09-3); B-12 methyl N-[[2-(3-pyridyl)indazole-5-carbonyl]amino]carbamate (CAS 1680188-56-0); B-13 2-(3-pyridyl)-N-(tetrahydrofuran-2-ylmethyl)indazole-5-carboxamide (CAS 1680188-55-9); B-14 N-[(2,2-difluorocyclopropyl)methyl]-2-(3-pyridyl)indazole-5-carboxamide (CAS 1680188-65-1); B-15 N-(2,2-difluoropropyl)-2-(3-pyridyl)indazole-5-carboxamide (CAS 1680188-68-4); B-16 2-(3-pyridyl)-N-(pyrimidin-2-ylmethyl)indazole-5-carboxamide (CAS 1680188-69-5); B-17 N-[(5-methylpyrazin-2-yl)methyl]-2-(3-pyridyl)indazole-5-carboxamide (CAS 1680188-91-3); B-18 indazapyroxamet; B-19 N-[1-(difluoromethyl)cyclopropyl]-2-(3-pyridyl)indazole-4-carboxamide (CAS 2408908-90-5); B-20 N-(1,1-dimethyl-2-methylsulfanyl-ethyl)-7-fluoro-2-(3-pyridyl)indazole-4-carboxamide (CAS 2408908-91-6); B-21 N-(1,1-dimethyl-2-methylsulfinyl-ethyl)-7-fluoro-2-(3-pyridyl)indazole-4-carboxamide (CAS 2408908-92-7); B-22 1,1-dimethyl-2-methylsulfonyl-ethyl)-7-fluoro-2-(3-pyridyl)indazole-4-carboxamide (CAS 2408908-93-8); B-23 cyproflanilide (CAS 2375110-88-4); B-24 flupentiofenox (CAS 1472050-04-6); B-25 cyetpyrafen (CAS 1253429-01-4); B-26 flufenerim (CAS 170015-32-4); B-27 (2Z)-2-[2-fluoro-4-methyl-5-(2,2,2-trifluoroethylsulfinyl)phenyl]imino-3-(2,2,2-trifluoroethyl)thiazolidin-4-one (CAS 1445683-71-5 (no specific stereochemistry)); B-27a (R)-(2Z)-2-[2-fluoro-4-methyl-5-(2,2,2-trifluoroethylsulfinyl)phenyl]imino-3-(2,2,2-trifluoroethyl)thiazolidin-4-one (CAS 2377084-09-6 (R-enantiomer)); B-28 1-[6-(2,2-difluoro-7-methyl-[1,3]dioxolo[4,5-f]benzimidazol-6-yl)-5-ethylsulfonyl-3-pyridyl]cyclopropanecarbonitrile (CAS 2408220-94-8); B-29 2-(5-cyclopropyl-3-ethylsulfonyl-2-pyridyl)-5-(trifluoromethylsulfonyl)-1,3-benzoxazole (CAS 2128706-05-6); B-30 2-[3-ethylsulfonyl-6-(1,2,4-triazol-1-yl)-2-pyridyl]-3-methyl-6-(trifluoromethyl)imidazo[4,5-b]pyridine (CAS 1808115-49-2); B-31 5-[[(1R,3R)-3-[3,5-bis(trifluoromethyl)phenyl]-2,2-dichloro-cyclopropanecarbonyl]amino]-2-chloro-N-[3-[(2,2-difluoroacetyl)amino]-2,4-difluoro-phenyl]benzamide) (CAS 2220132-55-6); B-32 5-[[trans-3-[3,5-bis(trifluoromethyl)phenyl]-2,2-dichloro-cyclopropanecarbonyl]amino]-2-chloro-N-[3-[(2,2-difluoroacetyl)amino]-2,4-difluoro-phenyl]benzamide; B-33 N-[4-chloro-2-(3-pyridyl)thiazol-5-yl]-N-ethyl-3-methylsulfonyl-propanamide (CAS 1255091-74-7); B-34 fluchlordiniliprole; and B-35 5-bromo-2-(3-chloro-2-pyridyl)-N-[2,4-dichloro-6-(isopropylcarbamothioyl)phenyl]pyrazole-3-carboxamide (CAS 1442448-92-1) or agrochemically acceptable salts thereof.

2. The method according to claim 1, wherein the ratio of (A) to (B) by weight is from 500:1 to 1:500, 100:1 to 1:100, 50:1 to 1:50, or 20:1 to 1:20, or preferably 10:1 to 1:10 or 5:1 to 1:5; more preferably 3:1 to 1:3.

3. A method of controlling or preventing damage to a plant, which comprises applying, in any desired sequence or simultaneously, on the plant, the locus thereof or its propagation material, a combination comprising, as component (A), a compound of formula (I): ##STR00008## or an agrochemically acceptable salt thereof, and, as component (B), a compound selected from the group consisting of: B-36 thiacloprid (CAS 111988-49-9); B-37 imidacloprid (CAS 138261-41-3); B-38 flupyradifurone (CAS 951659-40-8); B-39 triflumezopyrim (CAS 1263133-33-0); B-40 sulfoxaflor (CAS 946578-00-3); B-41 acetamiprid (CAS 135410-20-7); B-42 nitenpyram (CAS 150824-47-8); B-43 clothianidin (CAS 210880-92-5); B-44 thiamethoxam (CAS 153719-23-4); B-45 tetraniliprole (CAS 1229654-66-3); B-46 flubendiamide (CAS 272451-65-7); B-47 chlorantraniliprole (CAS 500008-45-7); B-48 cyantraniliprole (CAS 500008-45-7); B-49 pyridalyl (CAS 179101-81-6); B-50 indoxacarb (CAS 173584-44-6); B-51 deltamethrin (CAS 52918-63-5); B-52 cyfluthrin (CAS 68359-37-5); B-53 bifenthrin (CAS 82657-04-3); B-54 zeta-cypermethrin (CAS 1315501-18-8); B-55 gamma-cyhalothrin (CAS 76703-62-3); B-56 acrinathrin (CAS 101007-06-1); B-57 permethrin (CAS 52645-53-1); B-58 alpha-cypermethrin (CAS 67375-30-8); B-59 fenpropathrin (CAS 39515-41-8); B-60 fenvalerate (CAS 51630-58-1); B-61 esfenvalerate (CAS 66230-04-4); B-62 cypermethrin (CAS 52315-07-8); and B-63 lambda-cyhalothrin (CAS 91465-08-6); or agrochemically acceptable salts thereof, wherein the combination of (A) and (B) is in a ratio by weight of 500:1 to 1:500, 100:1 to 1:100, 50:1 to 1:50, or 20:1 to 1:20, or preferably 10:1 to 1:10 or 5:1 to 1:5; more preferably 3.1 to 1:3.

4. The method according to claim 1 wherein the combination is applied in succession, or simultaneously or as a composition comprising (A) and (B).

5. The method according to claim 1 wherein the ratio of (A) to (B) by weight is from 1 to 2:2.5 to 1, especially from 2:1 to 1:2, advantageously about 1:1.

6. The method according to claim 1, wherein the rate of application of (A) is 1 to 2000 g/ha, especially from 10 to 1000 g/ha and (B) is 1 to 2000 g/ha, especially from 10 to 1000 g/ha; or the rate of application of (A) is from 20 to 100 g/ha and (B) is 20 to 200 g/ha.

7. The method according to claim 1, wherein the combination controls one or more pests selected from insect, nematode and acarina pests.

8. A composition comprising, as component (A), a compound of formula (I): ##STR00009## or an agrochemically acceptable salt thereof; and, as component (B), a compound selected from the group consisting of: B-1 nicofluprole (CAS 1771741-86-6); B-2 spidoxamat (CAS 907187-07-9); B-3 N-[[2-fluoro-4-[2-hydroxy-3-(3,4,5-trichlorophenyl)-3-(trifluoromethyl)pyrrolidin-1-yl]phenyl]methyl]cyclopropanecarboxamide (CAS 1365070-72-9); B-3a (S)N-[[2-fluoro-4-[2-hydroxy-3-(3,4,5-trichlorophenyl)-3-(trifluoromethyl)pyrrolidin-1-yl]phenyl]methyl]cyclopropanecarboxamide (CAS 2044701-44-0); B-4 4-dimethyl-2-[2-(3-pyridyl)indazol-5-yl]-1,2,4-triazolidine-3,5-dione (CAS 2171099-09-3); B-5 N-[3-chloro-1-(3-pyridyl)pyrazol-4-yl]-2-methylsulfonyl-propanamide (CAS 2396747-83-2); B-6 (3R)-3-(2-chlorothiazol-5-yl)-8-methyl-5-oxo-6-phenyl-2,3-dihydrothiazolo[3,2-a]pyrimidin-8-ium-7-olate (CAS 2246757-58-2); B-7 1-(2-cyanoethyl)-4-oxo-3-[3-(trifluoromethyl)phenyl]pyrido[1,2-a]pyrimidin-1-ium-2-olate (CAS 2032403-97-5); B-8 N-isopropyl-2-(3-pyridyl)indazole-4-carboxamide (CAS 1680187-98-7); B-9 N-cyclopropyl-2-(3-pyridyl)indazole-4-carboxamide (CAS 1680188-04-8); B-10 N-cyclohexyl-2-(3-pyridyl)indazole-4-carboxamide (CAS 1680188-06-0); B-11 2-(3-pyridyl)-N-(2,2,2-trifluoroethyl)indazole-4-carboxamide (CAS 1680188-09-3); B-12 methyl N-[[2-(3-pyridyl)indazole-5-carbonyl]amino]carbamate (CAS 1680188-56-0); B-13 2-(3-pyridyl)-N-(tetrahydrofuran-2-ylmethyl)indazole-5-carboxamide (CAS 1680188-55-9); B-14 N-[(2,2-difluorocyclopropyl)methyl]-2-(3-pyridyl)indazole-5-carboxamide (CAS 1680188-65-1); B-15 N-(2,2-difluoropropyl)-2-(3-pyridyl)indazole-5-carboxamide (CAS 1680188-68-4); B-16 2-(3-pyridyl)-N-(pyrimidin-2-ylmethyl)indazole-5-carboxamide (CAS 1680188-69-5); B-17 N-[(5-methylpyrazin-2-yl)methyl]-2-(3-pyridyl)indazole-5-carboxamide (CAS 1680188-91-3); B-18 indazapyroxamet (CAS 1689545-27-4); B-19 N-[1-(difluoromethyl)cyclopropyl]-2-(3-pyridyl)indazole-4-carboxamide (CAS 2408908-90-5); B-20 N-(1,1-dimethyl-2-methylsulfanyl-ethyl)-7-fluoro-2-(3-pyridyl)indazole-4-carboxamide (CAS 2408908-91-6); B-21 N-(1,1-dimethyl-2-methylsulfinyl-ethyl)-7-fluoro-2-(3-pyridyl)indazole-4-carboxamide (CAS 2408908-92-7); B-22 1,1-dimethyl-2-methylsulfonyl-ethyl)-7-fluoro-2-(3-pyridyl)indazole-4-carboxamide (CAS 2408908-93-8); B-23 cyproflanilide (CAS 2375110-88-4); B-24 flupentiofenox (CAS 1472050-04-6); B-25 cyetpyrafen (CAS 1253429-01-4); B-26 flufenerim (CAS 170015-32-4); B-27 (2Z)-2-[2-fluoro-4-methyl-5-(2,2,2-trifluoroethylsulfinyl)phenyl]imino-3-(2,2,2-trifluoroethyl)thiazolidin-4-one (CAS 1445683-71-5 (no specific stereochemistry)); B-27a (R)-(2Z)-2-[2-fluoro-4-methyl-5-(2,2,2-trifluoroethylsulfinyl)phenyl]imino-3-(2,2,2-trifluoroethyl)thiazolidin-4-one (CAS 2377084-09-6 (R-enantiomer)); B-28 1-[6-(2,2-difluoro-7-methyl-[1,3]dioxolo[4,5-f]benzimidazol-6-yl)-5-ethylsulfonyl-3-pyridyl]cyclopropanecarbonitrile (CAS 2408220-94-8); B-29 2-(5-cyclopropyl-3-ethylsulfonyl-2-pyridyl)-5-(trifluoromethylsulfonyl)-1,3-benzoxazole (CAS 2128706-05-6); B-30 2-[3-ethylsulfonyl-6-(1,2,4-triazol-1-yl)-2-pyridyl]-3-methyl-6-(trifluoromethyl)imidazo[4,5-b]pyridine (CAS 1808115-49-2); B-31 5-[[(1R,3R)-3-[3,5-bis(trifluoromethyl)phenyl]-2,2-dichloro-cyclopropanecarbonyl]amino]-2-chloro-N-[3-[(2,2-difluoroacetyl)amino]-2,4-difluoro-phenyl]benzamide) (CAS 2220132-55-6) B-32 5-[[trans-3-[3,5-bis(trifluoromethyl)phenyl]-2,2-dichloro-cyclopropanecarbonyl]amino]-2-chloro-N-[3-[(2,2-difluoroacetyl)amino]-2,4-difluoro-phenyl]benzamide; B-33 N-[4-chloro-2-(3-pyridyl)thiazol-5-yl]-N-ethyl-3-methylsulfonyl-propanamide (CAS 1255091-74-7); B-34 fluchlordiniliprole; and B-35 5-bromo-2-(3-chloro-2-pyridyl)-N-[2,4-dichloro-6-(isopropylcarbamothioyl)phenyl]pyrazole-3-carboxamide (CAS 1442448-92-1); or agrochemically acceptable salts thereof.

9. The composition according to claim 8, wherein the ratio of (A) to (B) by weight is from 500:1 to 1:500, 100:1 to 1:100, 50:1 to 1:50, or 20:1 to 1:20, or preferably 10:1 to 1:10 or 5:1 to 1:5; more preferably 3:1 to 1:3.

10. A composition comprising, as component (A), a compound of formula (I): ##STR00010## or an agrochemically acceptable salt thereof; and, as component (B), a compound selected from the group consisting of: B-36 thiacloprid (CAS 111988-49-9); B-37 imidacloprid (CAS 138261-41-3); B-38 flupyradifurone (CAS 951659-40-8); B-39 triflumezopyrim (CAS 1263133-33-0); B-40 sulfoxaflor (CAS 946578-00-3); B-41 acetamiprid (CAS 135410-20-7); B-42 nitenpyram (CAS 150824-47-8); B-43 clothianidin (CAS 210880-92-5); B-44 thiamethoxam (CAS 153719-23-4); B-45 tetraniliprole (CAS 1229654-66-3); B-46 flubendiamide (CAS 272451-65-7); B-47 chlorantraniliprole (CAS 500008-45-7); B-48 cyantraniliprole (CAS 500008-45-7); B-49 pyridalyl (CAS 179101-81-6); B-50 indoxacarb (CAS 173584-44-6); B-51 deltamethrin (CAS 52918-63-5); B-52 cyfluthrin (CAS 68359-37-5); B-53 bifenthrin (CAS 82657-04-3); B-54 zeta-cypermethrin (CAS 1315501-18-8); B-55 gamma-cyhalothrin (CAS 76703-62-3); B-56 acrinathrin (CAS 101007-06-1); B-57 permethrin (CAS 52645-53-1); B-58 alpha-cypermethrin (CAS 67375-30-8); B-59 fenpropathrin (CAS 39515-41-8); B-60 fenvalerate (CAS 51630-58-1); B-61 esfenvalerate (CAS 66230-04-4); B-62 cypermethrin (CAS 52315-07-8); and B-63 lambda-cyhalothrin (CAS 91465-08-6); or agrochemically acceptable salts thereof, wherein the composition comprises (A) and (B) in a ratio by weight of 500:1 to 1:500, 100:1 to 1:100, 50:1 to 1:50, or 20:1 to 1:20, or preferably 10:1 to 1:10 or 5:1 to 1:5; more preferably 3.1 to 1:3.

11. The composition according to claim 8 further comprising one or more auxiliaries and/or diluents.

12. The composition according to claim 8 wherein the ratio of (A) to (B) by weight is from 1 to 2:2.5 to 1, especially from 2:1 to 1:2, advantageously about 1:1.

13. A formulation comprising a composition according to claim 8, the formulation comprising from 0.01 to 90% by weight of active agents, from 0 to 25% by weight of agriculturally acceptable surfactant and from 10 to 99.9 percent by weight of solid or liquid formulation inerts and adjuvants.

14. A concentrated composition for dilution by a user, comprising a composition according to claim 8, comprising from 2 to 80% by weight, preferably between about 5 and 70% by weight of active ingredients.

15. A method of controlling or preventing damage to a plant, which comprises applying to a seed, in any desired sequence or simultaneously, as component (A), a compound of formula (I): ##STR00011## or an agrochemically acceptable salt thereof, and, as component (B), a compound selected from the group consisting of B-1, B-3, B-3a, B-4 to B-7, B-18, B-23, B-31, B-33, B-36 to B-45, and B-47 to B-48, and/or a compound selected from the group consisting of: TABLE-US-00023 B-64 iprodione B-65 fluindapyr B-66 flutriafol B-67 amisulbrom B-68 fluopicolide B-69 thiram B-70 carboxin B-71 thiophanate-methyl B-72 dimethomorph B-73 fluquinconazole B-74 myclobutanil B-75 cyproconazole B-76 fluazinam B-77 cymoxanil B-78 cyazofamid B-79 hymexazole B-80 fluoxapiprolin B-81 cyprodinil B-82 florylpicoxamid B-83 oxadixyl B-84 acibenzolar-S-methyl B-85 bixafen B-86 flutolanil B-87 pyraziflumid B-88 pyrapropoyne B-89 zoxamide B-90 prochloraz B-91 triadimenol B-92 imazalil B-93 oxpoconazole B-94 enestrobin B-95 metyltetraprole B-96 quinofumelin B-97 cyflufenamid; and B-98 ipflufenoquin or agrochemically acceptable salts thereof.

16. The method according to claim 1, wherein the plant expresses or contains one or more toxins that impart to the plant tolerance or resistance to harmful insects, wherein preferably the toxin is selected from S-endotoxins, such as Cry1Ab, Cry1Ac, Cry1F, Cry1Fa2, Cry2Ab, Cry3A, Cry3Bb1 and Cry9C, vegetative insecticidal proteins (Vip), such as Vip1, Vip2, Vip3 and Vip3A, hybrid toxins, truncated toxins and modified toxins, more preferably is selected from Cry1Ab and Cry1Ac.

17. A seed treatment composition comprising, as component (A), a compound of formula (I) as defined above or an agrochemically acceptable salt thereof; and, as component (B) at least one compound selected from the group consisting of B-1, B-3, B-3a, B-4 to B-7, B-18, B-23, B-31, B-33, B-36 to B-45, B-47 to B-48 and B-64 to B-98 as defined above, or agrochemically acceptable salts thereof.

18. A seed treatment composition as claimed in claim 17 comprising 0.001 to 50 g of component (A) per kg of seed, preferably from 0.01 to 10 g per kg of seed.

19. A seed treatment composition as claimed in claim 17 comprising 0.001 to 50 g of the or each compound of component (B), per kg of seed, preferably from 0.01 to 10 g per kg of seed.

20. Use of a composition as defined in claim 8 as an insecticide, nematicide or acaricide or for controlling insect, nematode or acarina pests.

Description

BIOLOGICAL EXAMPLES

[0265] The synergistic biological activity of combinations of component (A) and component (B) as disclosed herein was assessed using a Spodoptera littoralis contact assay. Herein, cotton leaf discs were placed onto agar in 24-well microtiter plates and sprayed with aqueous test solutions prepared from 10,000 ppm DMSO stock solutions. After drying the leaf discs were infested with L1 larvae. The samples were assessed for mortality 3 days after infestation.

[0266] The tables below present the observed mortality relative to the expected mortality as determined using the COLBY formula for various combinations of compound A and compound B as disclosed herein. If the mortality actually observed is greater than the expected mortality, then the action of the combination is super-additive, i.e. there is a synergistic effect.

TABLE-US-00013 Component A Component B Mortality (%) A B-2 Expected Observed [AI] (mg/L) [AI] (mg/L) (%) (%) 5 15.625 90 0 2.5 7.812 0 10 1.25 3.906 0 0

TABLE-US-00014 Component A Component B Mortality (%) A B-3a Expected Observed [AI] (mg/L) [AI] (mg/L) (%) (%) 10 0.062 100 100 5 0.031 90 100 2.5 0.016 0 50 1.25 0.008 0 10 0.625 0.004 0 0 0.312 0.002 0 0 2.5 0.062 0 50 1.25 0.031 0 0 0.625 0.016 0 0 0.312 0.008 0 0 2.5 0.25 0 50 1.25 0.125 0 20 0.625 0.062 0 0 0.312 0.031 0 0 1.25 0.5 90 100 0.625 0.25 70 100 0.312 0.125 10 80 0.156 0.062 0 60 0.078 0.031 0 20 0.039 0.016 0 0

TABLE-US-00015 Component A Component B Mortality (%) A B-5 Expected Observed [AI] (mg/L) [AI] (mg/L) (%) (%) 10 31.25 100 100 5 15.625 90 100 2.5 7.812 0 10 2.5 31.25 0 10 1.25 15.625 0 0 0.625 7.812 0 0

TABLE-US-00016 Component A Component B Mortality (%) A B-7 Expected Observed [AI] (mg/L) [AI] (mg/L) (%) (%) 2.5 7.812 0 10 1.25 3.906 0 0 2.5 31.25 0 50 1.25 15.625 0 0 2.5 125 0 10 1.25 62.5 0 0

TABLE-US-00017 Component A Component B Mortality (%) A B-35 Expected Observed [AI] (mg/L) [AI] (mg/L) (%) (%) 5 62.5 90 30 2.5 31.25 0 40 1.25 15.625 0 20 0.625 7.812 0 0

TABLE-US-00018 Component A Component B Mortality (%) A B-36 Expected Observed [AI] (mg/L) [AI] (mg/L) (%) (%) 10 31.25 100 100 5 15.625 90 90 2.5 7.812 0 50 1.25 3.906 0 10 0.625 1.953 0 0 2.5 125 20 30 1.25 62.5 0 20 0.625 31.25 0 10 1.25 250 60 70 0.625 125 20 60 0.312 62.5 0 50 0.156 31.25 0 0

TABLE-US-00019 Component A Component B Mortality (%) A B-37 Expected Observed [AI] (mg/L) [AI] (mg/L) (%) (%) 10 31.25 100 100 5 15.625 90 90 2.5 7.812 0 90 1.25 3.906 0 0

TABLE-US-00020 Component A Component B Mortality (%) A B-44 Expected Observed [AI] (mg/L) [AI] (mg/L) (%) (%) 10 0.5 100 100 5 0.25 92 100 2.5 0.125 0 20 1.25 0.062 0 0 0.625 0.031 0 0 5 1 100 100 2.5 0.5 100 100 1.25 0.25 20 80 0.625 0.125 0 50 0.312 0.062 0 10 0.156 0.031 0 0 0.625 0.5 100 100 0.312 0.25 20 90 0.156 0.125 0 40 0.078 0.062 0 0 0.312 1 100 100 0.156 0.5 100 100 0.078 0.25 20 100 0.039 0.125 0 60

TABLE-US-00021 Component A Component B Mortality (%) A B-47 Expected Observed [AI] (mg/L) [AI] (mg/L) (%) (%) 2.5 7.5 100 100 1.25 3.75 90 100 0.625 1.875 20 50 0.312 0.938 20 10

TABLE-US-00022 Component A Component B Mortality (%) A B-55 Expected Observed [AI] (mg/L) [AI] (mg/L) (%) (%) 5 0.625 90 80 2.5 0.312 0 20 1.25 0.156 0 10 0.625 0.078 0 0 0.312 0.039 0 0 2.5 1.25 40 40 1.25 0.625 0 10 0.625 0.312 0 0 0.312 0.156 0 0 0.625 1.25 40 70 0.312 0.625 0 20 0.156 0.312 0 0 0.312 2.5 90 100 0.156 1.25 40 60 0.078 0.625 0 30