ALKYL CARBOXYLIC ACID COMPOUNDS AND APPLICATION THEREOF

Abstract

A series of alkyl carboxylic acid compounds and an application thereof, and relates in particular to compounds represented by formula (II) and a pharmaceutically acceptable salt thereof.

##STR00001##

Claims

1. A compound of formula (II) or a pharmaceutically acceptable salt thereof, ##STR00067## wherein R.sub.1 is selected from R.sub.2, ##STR00068## and -L-R.sub.3; R.sub.2 is selected from cyclopentyl, C.sub.4-6 bicycloalkyl, and 4- to 5-membered heterocycloalkyl, and the cyclopentyl, C.sub.4-6 bicycloalkyl, and 4- to 5-membered heterocycloalkyl are each independently and optionally substituted by 1, 2, or 3 R.sub.a; R.sub.3 is selected from C.sub.3-6 cycloalkyl and 4- to 5-membered heterocycloalkyl, and the C.sub.3-6 cycloalkyl and 4- to 5-membered heterocycloalkyl are each independently and optionally substituted by 1, 2, or 3 R.sub.b; L is selected from O, OCH.sub.2, CH(R.sub.c), and C(R.sub.dR.sub.e); R.sub.a and R.sub.b are each independently selected from halogen, CN, C.sub.1-3 alkyl, and C.sub.1-3 alkoxy, and the C.sub.1-3 alkyl and C.sub.1-3 alkoxy are each independently and optionally substituted by 1, 2, or 3 R.sub.f; R.sub.c, R.sub.d, and R.sub.e are each independently selected from halogen and C.sub.1-3 alkyl; R.sub.f is selected from halogen; R.sub.4 is selected from halogen and C.sub.1-3 alkyl; T.sub.1 is selected from CH and N; hetero in the heterocycloalkyl represents 1, 2, or 3 heteroatoms or heteroatom groups, each independently selected from O, NH, S, and N.

2. The compound or the pharmaceutically acceptable salt thereof according to claim 1, wherein R.sub.a and R.sub.b are each independently selected from F and methoxy.

3. The compound or the pharmaceutically acceptable salt thereof according to claim 1, wherein R.sub.c, R.sub.d, and R.sub.e are each independently selected from F and methyl.

4. The compound or the pharmaceutically acceptable salt thereof according to claim 1, wherein R.sub.f is selected from F.

5. The compound or the pharmaceutically acceptable salt thereof according to claim 1, wherein R.sub.2 is selected from ##STR00069## and the ##STR00070## are each independently and optionally substituted by 1, 2, or 3 R.sub.a.

6. The compound or the pharmaceutically acceptable salt thereof according to claim 5, wherein R.sub.2 is selected from ##STR00071##

7. The compound or the pharmaceutically acceptable salt thereof according to claim 1, wherein R.sub.3 is selected from ##STR00072## and the ##STR00073## are optionally substituted by 1, 2, or 3 R.sub.b.

8. The compound or the pharmaceutically acceptable salt thereof according to claim 7, wherein R.sub.3 is selected from ##STR00074##

9. The compound or the pharmaceutically acceptable salt thereof according to claim 1, wherein L is selected from O, OCH.sub.2, and C(CH.sub.3).sub.2.

10. The compound or the pharmaceutically acceptable salt thereof according to claim 1, wherein R.sub.1 is selected from ##STR00075##

11. The compound or the pharmaceutically acceptable salt thereof according to claim 1, wherein R.sub.4 is selected from F, Cl, and methyl.

12. The compound or the pharmaceutically acceptable salt thereof according to claim 1, wherein the compound is selected from: ##STR00076##

13. A compound of the following formula or a pharmaceutically acceptable salt thereof, selected from: ##STR00077## ##STR00078## ##STR00079## ##STR00080##

14. The compound or the pharmaceutically acceptable salt thereof according to claim 13, selected from: ##STR00081## ##STR00082## ##STR00083## ##STR00084##

15. The compound or the pharmaceutically acceptable salt thereof according to claim 14, selected from: ##STR00085## ##STR00086## ##STR00087## ##STR00088## ##STR00089##

16. A method for treating a disease related to soluble guanylate cyclase activators in a subject in need thereof, comprising: administering the compound or the pharmaceutically acceptable salt thereof according to claim 1 to the subject.

17. The method according to claim 16, wherein the disease related to soluble guanylate cyclase activators is chronic kidney disease.

Description

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENT

[0094] The present disclosure is described in detail by the examples below, but it does not mean that there are any adverse restrictions on the present disclosure. The present disclosure has been described in detail herein, and its specific examples have also been disclosed; for one skilled in the art, it is obvious to make various modifications and improvements to the examples of the present disclosure without departing from the spirit and scope of the present disclosure.

Example 1

[0095] ##STR00041##

Synthetic Route:

[0096] ##STR00042## ##STR00043##

Step 1: Synthesis of Compound WX001_2

[0097] tert-Butyl diethylphosphonoacetate (5.14 g, 20.38 mmol) was dissolved in tetrahydrofuran (30 mL) at room temperature under nitrogen atmosphere. The reaction system was cooled to 0? C., and potassium tert-butoxide (2.52 g, 22.48 mmol) was added thereto. After the reaction mixture was stirred for 30 minutes, WX001_1 (2 g, 20.38 mmol) was slowly added dropwise thereto. Once the addition was complete, the reaction system returned to room temperature and was stirred for 12 hours. After the reaction was complete, the reaction mixture was diluted with water (50 mL), and then extracted with ethyl acetate (60 mL?3). The organic phases were combined. The organic phase was washed with saturated brine (60 mL?2), dried over anhydrous sodium sulfate, filtered, and then concentrated under reduced pressure to remove the solvent. The resulting crude product was separated by column chromatography (eluent: petroleum ether/ethyl acetate=1/0 to 1/1, v/v) to obtain compound WX001_2. .sup.1H NMR (400 MHz, DMSO_d.sub.6) 6: 6.75 (dd, J=8.0, 15.5 Hz, 1H), 5.75-5.65 (m, 1H), 2.62-2.52 (m, 1H), 1.83-1.72 (m, 2H), 1.65-1.53 (m, 4H), 1.42 (s, 9H), 1.36-1.28 (m, 2H).

Step 2: Synthesis of Compound WX001_4

[0098] 2,2-Bis(diphenylphosphino)-1,1-binaphthalene (18.24 mg, 29.29 ?mol) and chloro(1,5-cyclooctadiene)rhodium(I) dimer (62.80 mg, 127.37 ?mol) were dissolved in tetrahydrofuran (10 mL) at room temperature under nitrogen atmosphere. The reaction system was stirred at room temperature for 15 minutes. Compound WX001_3 (1.42 g, 5.60 mmol) was dissolved in isopropanol (5 mL) and tetrahydrofuran (10 mL). Compound WX001_2 (1.00 g, 5.09 mmol), potassium hydroxide (343.00 mg, 6.11 mmol), and 1,5-cyclooctadiene (55.11 mg, 509.46 ?mol) were sequentially added thereto. The reaction system was heated to 60? C., added with the prepared catalyst, and stirred at 60? C. for 9 hours. After the reaction was complete, the reaction mixture was cooled to room temperature, diluted with water (150 mL), and then extracted with ethyl acetate (150 mL?3). The organic phases were combined. The organic phase was washed with saturated brine (100 mL?2), dried over anhydrous sodium sulfate, filtered, and then concentrated under reduced pressure to remove the solvent. The resulting crude product was separated by column chromatography (eluent: petroleum ether/ethyl acetate=1/0 to 1/1, v/v) to obtain compound WX001_4. .sup.1H NMR (400 MHz, CDCl.sub.3) ?: 7.12 (d, J=8.1 Hz, 1H), 6.60 (d, J=2.0 Hz, 1H), 6.52 (dd, J=2.0, 8.3 Hz, 1H), 3.98 (s, 2H), 2.73-2.61 (m, 2H), 2.45-2.35 (m, 1H), 1.97-1.78 (m, 2H), 1.70-1.61 (m, 1H), 1.59-1.49 (m, 2H), 1.45-1.35 (m, 2H), 1.28-1.25 (m, 9H), 1.08-0.96 (m, 1H), 0.91-0.82 (m, 1H).

Step 3: Synthesis of Compound WX001_7

[0099] Diisopropylamine (31.52 g, 311.45 mmol, 44.02 mL) was placed in a dry reaction flask at room temperature under nitrogen atmosphere. Tetrahydrofuran (350 mL) was added to dissolve the compound. The reaction system was cooled to ?70? C., and a solution of n-butyllithium in hexane (130.00 mL, 324.99 mmol, 2.5 M) was added thereto. The reaction system was stirred at ?70? C. for 30 minutes, then warmed to ?40? C., and stirred for another 30 minutes. The reaction system was cooled to ?70? C., and WX001_6 (50 g, 270.83 mmol) dissolved in tetrahydrofuran (120 mL) was added thereto. The reaction system was stirred at ?70? C. for 1 hour. Trifluoroacetone (45.52 g, 406.24 mmol) dissolved in tetrahydrofuran (150 mL) was then added, and the reaction system was stirred at ?70? C. for another 2 hours. After the reaction was complete, the reaction system was warmed to 0? C., added with 120 mL of 5 N dilute hydrochloric acid to quench the reaction, and then stirred at room temperature for 30 minutes. The reaction mixture was concentrated under reduced pressure to remove most of the organic solvent, and the residue was diluted with ethyl acetate (300 mL). The phases were separated, and the organic phase was collected. The aqueous phase was extracted with ethyl acetate (200 mL?3), and the organic phases were combined. The organic phase was washed with saturated brine (200 mL?2), dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to remove the solvent to obtain compound WX001_7.

Step 4: Synthesis of Compound WX001_8

[0100] WX001_7 (80.35 g, 270.84 mmol) was dissolved in pyridine (160 mL) at room temperature under nitrogen atmosphere. To the reaction system at room temperature was added phosphorus oxychloride (49.83 g, 325.01 mmol). The reaction system was stirred at 110? C. for 12 hours. After the reaction was complete, the reaction system was cooled to room temperature, poured into 200 mL of water to quench the reaction, added with 6 N dilute hydrochloric acid to adjust the pH to around 3, and diluted with dichloromethane (400 mL). The phases were separated, and the organic phase was collected. The aqueous phase was extracted with dichloromethane (300 mL?3), and the organic phases were combined. The organic phase was washed with saturated brine (200 mL?2), dried over anhydrous sodium sulfate, filtered, and then concentrated under reduced pressure to remove the solvent. The resulting crude product was separated by column chromatography (eluent: petroleum ether/ethyl acetate=1/0 to 9/1, v/v) to obtain a mixture of compounds WX001_8 and WX001_9. The mixture was then separated by column chromatography (eluent: petroleum ether/ethyl acetate=1/0 to 9/1, v/v) to obtain compound WX001_8.

Step 5: Synthesis of Compound WX001_9

[0101] Diisopropylamine (7.99 g, 78.95 mmol) was placed in a dry reaction flask and dissolved in tetrahydrofuran (50 mL) at room temperature under nitrogen atmosphere. The reaction system was replaced with nitrogen three times and then cooled to ?60? C. A solution of n-butyllithium in hexane (71.77 mmol, 28.71 mL, 2.5 M) was slowly added thereto. The reaction system was warmed to ?40? C. and stirred at ?40? C. for 30 minutes. Afterwards, the reaction system was cooled to ?65? C., and WX001_8 (10 g, 35.89 mmol) dissolved in tetrahydrofuran (50 mL) was added dropwise thereto. The reaction system was stirred at ?65? C. for another 1.5 hours. After the reaction was complete, a solution of acetic acid (8.62 g, 143.55 mmol) in tetrahydrofuran (40 mL) was added to the reaction system to quench the reaction. The reaction mixture was then stirred at room temperature for 2 hours. The reaction mixture was diluted with water (80 mL) and ethyl acetate (80 mL), and then the phases were separated. The organic phase was collected and the aqueous phase was extracted with ethyl acetate (80 mL?3). The organic phases were then combined. The organic phase was washed with saturated brine (80 mL?2), dried over anhydrous sodium sulfate, filtered, and then concentrated under reduced pressure to remove the solvent. The resulting crude product was separated by column chromatography (eluent: petroleum ether/ethyl acetate=1/0 to 9/1, v/v) to obtain compound WX001_9. The crude product was directly used in the next step.

Step 6: Synthesis of Compound WX001_10

[0102] Pt/C (0.57 g, purity: 5%) was added to a dry hydrogenation flask and wetted with methanol (60 mL) at room temperature under argon atmosphere. WX001_9 (5.7 g, 20.46 mmol) was then added to the reaction system. The reaction system was stirred at 50? C. under hydrogen atmosphere of 50 psi for 12 hours. Upon completion of the reaction, the reaction system was filtered through diatomite. The filter cake was washed with methanol (60 mL?5), and the filtrates were combined and concentrated under reduced pressure to obtain the residue. Compound WX001_10 was obtained.

Step 7: Synthesis of Compound WX001_11

[0103] WX001_10 (5.8 g, 20.66 mmol) was dissolved in methanol (60 mL) at room temperature under nitrogen atmosphere. To the reaction system was then added sodium methoxide (3.35 g, 61.99 mmol). The reaction system was stirred at 80? C. for 12 hours. Additional sodium methoxide (1.12 g, 20.66 mmol) was added thereto, and the reaction system was stirred at 80? C. for another 12 hours. The reaction mixture was heated to 90? C. and stirred for 12 hours. After the reaction was complete, the reaction mixture was cooled to room temperature, concentrated under reduced pressure to remove most of the organic solvent, diluted with water (60 mL), and then extracted with methyl tert-butyl ether (70 mL). The organic phase was discarded, the aqueous phase was added with 3 N dilute hydrochloric acid to adjust the pH to 2 to 3 and extracted with ethyl acetate (80 mL?3), and the organic phases were combined. The organic phase was washed with saturated brine (50 mL?2), dried over anhydrous sodium sulfate, filtered, and then concentrated under reduced pressure to remove the solvent. Compound WX001_11 was obtained.

Step 8: Synthesis of Compound WX001_5

[0104] Quinine (3.51 g, 10.83 mmol) was dissolved in ethanol (36 mL) at room temperature under nitrogen atmosphere. The reaction system was heated to 80? C., and WX001_11 (3.85 g, 14.44 mmol), dissolved in ethanol (19 mL), was gradually added dropwise thereto. The reaction system was stirred at 80? C. for 10 minutes. A white solid precipitated. The reaction system was slowly cooled to 0? C. and stirred at 0? C. for 1 hour. The reaction mixture was filtered, and the filter cake was washed with 5 mL of water. The solid was collected. The collected solid was dissolved in methanol (157 mL) and water (20 mL). The reaction system was then heated to 100? C. and stirred for 30 minutes. It was subsequently slowly cooled to room temperature, resulting in the precipitation of a white solid. The reaction mixture was filtered, and the filter cake was washed with 10 mL of water. The resulting filter cake was collected. The filter cake was dissolved in ethanol (120 mL) and water (20 mL). The reaction system was then heated to 100? C. and stirred for 30 minutes. It was subsequently slowly cooled to room temperature, resulting in the precipitation of a white solid. The filter cake was washed with 10 mL of water, and the solid was collected. The solid was suspended in water (25 mL) and hydrochloric acid (6 M, 3.25 mL) was added thereto. The reaction system was stirred at room temperature for 2.5 hours. The white solid was then filtered and the filter cake was washed with 1 mL of water. The solid was collected, and the filter cake was concentrated under reduced pressure to remove the solvent. Thus compound WX001_5 was obtained.

[0105] SFC analysis method: column type: Chiralpak IG-3 (100?4.6 mm I.D., 3 ?m); mobile phase: A: CO.sub.2, B: [MeOH (containing 0.1% IPAm)], gradient: B %: 5% to 40%, 3 minutes. The retention time for WX001_5 was recorded as 1.196 minutes.

Step 9: Synthesis of Compound WX001_12

[0106] Compound WX0015 (150 mg, 562.55 ?mol) was dissolved in dichloromethane (3 mL) at room temperature under nitrogen atmosphere. N,N-dimethylformamide (411.19 ?g, 5.63 ?mol) was added thereto, and the reaction system was cooled to 0? C. Oxalyl chloride (107.11 mg, 843.82 ?mol) was then added dropwise thereto. The reaction system was stirred at 25? C. for 1 hour, and concentrated under reduced pressure to remove the solvent. The residue was dissolved in dichloromethane (3 mL), and a solution of compound WX001_4 (218.62 mg, 675.06 ?mol) in dichloromethane (2 mL) was added dropwise thereto, along with N,N-diisopropylethylamine (145.41 mg, 1.13 mmol). The reaction system was stirred at room temperature for 2 hours. After the reaction was complete, the reaction mixture was concentrated under reduced pressure to remove the solvent. The resulting crude product was separated by column chromatography (eluent: petroleum ether/ethyl acetate=1/0 to 7/3, v/v) to obtain compound WX001_12. .sup.1H NMR (400 MHz, CDCl.sub.3) ?:8.17 (d, J=2.4 Hz, 1H), 7.58 (d, J=5.3 Hz, 1H), 7.42-7.31 (m, 4H), 7.21 (dd, J=2.2, 8.3 Hz, 1H), 6.88 (d, J=8.1 Hz, 1H), 3.68 (t, J=7.8 Hz, 1H), 3.47-3.34 (m, 1H), 2.84-2.76 (m, 1H), 2.74-2.64 (m, 1H), 2.49-2.39 (m, 1H), 2.02-1.92 (m, 1H), 1.90-1.80 (m, 1H), 1.68-1.61 (m, 1H), 1.59-1.49 (m, 2H), 1.41-1.31 (m, 2H), 1.30-1.27 (m, 1H), 1.23 (d, J=3.3 Hz, 9H), 1.09-1.01 (m, 1H), 0.96 (d, J=7.2 Hz, 3H).

Step 10: Synthesis of Compound WX001

[0107] Compound WX001_12 (150 mg, 262.02 ?mol) was dissolved in ethyl acetate (3 mL) in a pre-dried reaction flask at room temperature under nitrogen atmosphere. Hydrochloric acid in ethyl acetate (4 M, 3 mL) was added thereto, and the reaction system was stirred at room temperature for 10 hours. After the reaction was complete, the reaction mixture was concentrated under reduced pressure to remove the solvent. The resulting residue was separated by preparative HPLC (mobile phase: acetonitrile/water; acidic system: 0.04% HCl) to obtain compound WX001. MS-ESI m/z: 516.2 [M+H].sup.+. .sup.1H NMR (400 MHz, DMSO_d.sub.6) ?: 11.92 (s, 1H), 9.81 (s, 1H), 7.51-7.42 (m, 4H), 7.39-7.35 (m, 1H), 7.33 (d, J=8.3 Hz, 1H), 7.06-7.00 (m, 1H), 4.12 (d, J=10.7 Hz, 1H), 3.41-3.34 (m, 1H), 2.76-2.69 (m, 1H), 2.65 (dd, J=4.2, 15.6 Hz, 1H), 2.46-2.38 (m, 1H), 1.96-1.87 (m, 1H), 1.85-1.75 (m, 1H), 1.64-1.31 (m, 4H), 1.27-1.09 (m, 2H), 0.98-0.89 (m, 1H), 0.80 (d, J=7.0 Hz, 3H).

Example 2

[0108] ##STR00044##

Step 1: Synthesis of Compounds WX002 and WX003

[0109] Compound WX001 was separated by chiral column chromatography (column type: Phenomenex-Cellulose-2 (250 mm*30 mm, 10 ?m); mobile phase: A (CO.sub.2) and B (ethanol containing 0.1% ammonia); gradient: B %=30% to 30%, 5 minutes) to obtain compounds WX002 and WX003, respectively.

[0110] SFC analysis method: column type: Lux Cellulose-2, 50?4.6 mm I.D., 3 ?m; mobile phase: A: CO.sub.2, B: [EtOH (containing 0.1% IPAm)], gradient: B %: 5% to 50%, 3 minutes.

[0111] WX002 (retention time: 1.154 min): MS-ESI m/z: 516.2 [M+H].sup.+. ee value: 98.32%. .sup.1H NMR (400 MHz, DMSO_d.sub.6) ?: 9.82 (s, 1H), 7.46 (s, 4H), 7.40-7.28 (m, 2H), 7.03 (d, J=8.3 Hz, 1H), 4.12 (d, J=10.1 Hz, 1H), 3.45-3.41 (m, 1H), 2.72-2.60 (m, 2H), 2.45-2.30 (m, 1H), 1.99-1.74 (m, 2H), 1.65-1.32 (m, 4H), 1.29-1.08 (m, 2H), 0.93 (s, 1H), 0.80 (d, J=6.4 Hz, 3H).

[0112] WX003 (retention time: 1.416 min): MS-ESI m/z: 516.2 [M+H].sup.+. ee value: 95.24%. .sup.1H NMR (400 MHz, DMSO_d.sub.6) ?: 9.82 (s, 1H), 7.46 (s, 4H), 7.40-7.28 (m, 2H), 7.03 (d, J=8.3 Hz, 1H), 4.12 (d, J=10.1 Hz, 1H), 3.58-3.47 (m, 1H), 2.72-2.60 (m, 2H), 2.45-2.30 (m, 1H), 1.99-1.74 (m, 2H), 1.65-1.32 (m, 4H), 1.29-1.08 (m, 2H), 0.93 (s, 1H), 0.80 (d, J=6.4 Hz, 3H).

Example 4

[0113] ##STR00045##

Synthetic Route:

[0114] ##STR00046## ##STR00047##

Step 1: Synthesis of Compound WX004_2

[0115] Compound WX004_1 (7.8 g, 27.52 mmol) was dissolved in methanol (80 mL) at room temperature under nitrogen atmosphere. Iron powder (7.68 g, 137.59 mmol) was added thereto, followed by a dropwise addition of a solution of ammonium chloride (11.78 g, 220.14 mmol) in water (40 mL). The reaction system was stirred at 70? C. for 12 hours. After the reaction was complete, the reaction mixture was filtered. The filter cake was washed with methanol (50 mL?2). The filtrate was collected, and concentrated under reduced pressure to remove the solvent. The reaction mixture was diluted with water (50 mL) and then extracted with ethyl acetate (100 mL?2). The organic phases were combined. The organic phase was washed with saturated brine (50 mL?2), dried over anhydrous sodium sulfate, filtered, and then concentrated under reduced pressure to remove the solvent. The resulting residue was purified by column chromatography (eluent: petroleum ether/ethyl acetate=1/0 to 5/1, v/v) to obtain compound WX004_2.

Step 2: Synthesis of Compound WX004_3

[0116] To N,N-dimethylformamide (50 mL) was added sodium hydride (1.10 g, 27.42 mmol, purity: 60%) in batches at 0? C. under nitrogen atmosphere. A solution of compound WX004_2 (2.78 g, 10.97 mmol) in N,N-dimethylformamide (30 mL) was then slowly added dropwise to the reaction system. After the reaction system was stirred at 0? C. for 0.5 hours, 4-methoxybenzylchloride (4.09 g, 26.10 mmol, 3.55 mL) was added dropwise thereto. Following the addition, the reaction system was warmed to 25? C. and stirred for 12 hours. After the reaction was complete, the reaction system was slowly pouring into water (50 mL) to quench and diluted with ethyl acetate (100 mL), and the phases were separated. The organic phase was collected, and the aqueous phase was extracted with ethyl acetate (50 mL?3). The organic phases were then combined. The organic phase was washed with saturated brine (50 mL?2), dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated under reduced pressure to remove the solvent. The resulting residue was purified by column chromatography (eluent: petroleum ether/ethyl acetate=1/0 to 5/1, v/v) to obtain compound WX004_3. .sup.1H NMR (400 MHz, CDCl.sub.3) ?: 7.36-7.23 (m, 6H), 7.18 (d, J=8.4 Hz, 1H), 6.93 (d, J=8.5 Hz, 4H), 4.18 (s, 4H), 3.89 (s, 6H).

Step 3: Synthesis of Compound WX004_5

[0117] WX004_4 (500 mg, 3.84 mmol) and methoxymethylamine hydrochloride (412.32 mg, 4.23 mmol) were dissolved in ethyl acetate (10 mL) at room temperature under nitrogen atmosphere. N-Methylmorpholine (1.17 g, 11.53 mmol, 1.27 mL) was added dropwise thereto, followed by a dropwise addition of 1-propylphosphonic acid cyclic anhydride (3.67 g, 5.76 mmol, 3.43 mL, 50% solution in ethyl acetate). The reaction mixture was stirred at 20? C. for 12 hours. After the reaction was complete, the reaction mixture was poured into water (20 mL) to quench the reaction. The phases were separated, and the organic phase was collected. The aqueous phase was extracted with ethyl acetate (10 mL?2), and the organic phases were combined. The organic phase was washed with saturated brine (30 mL), dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated under reduced pressure to remove the solvent. The resulting residue was purified by column chromatography (eluent: petroleum ether/ethyl acetate=19/1 to 3/1, v/v) to obtain compound WX004_5. .sup.1H NMR (400 MHz, CDCl.sub.3) ?: 3.67 (s, 3H), 3.20 (s, 3H), 2.41 (d, J=2.5 Hz, 6H).

Step 4: Synthesis of Compound WX004_6

[0118] Compound WX004_3 (1.57 g, 3.18 mmol) was dissolved in tetrahydrofuran (17 mL) at room temperature under nitrogen atmosphere. The reaction mixture was cooled to ?70? C., and a 2 M solution of isopropylmagnesium chloride in tetrahydrofuran (1.59 mL, 3.18 mmol) was added dropwise thereto. After the addition was complete, the reaction mixture was warmed to ?40? C. and stirred for 0.5 hours. A solution of compound WX004_5 (220 mg, 1.27 mmol) in tetrahydrofuran (5 mL) was then added dropwise thereto. After the addition was complete, the reaction mixture was warmed to 20? C. and stirred for 12 hours. After the reaction was complete, the reaction mixture was slowly poured into saturated ammonium chloride solution (50 mL) to quench the reaction. The phases were separated, and the organic phase was collected. The aqueous phase was extracted with ethyl acetate (20 mL?2), and the organic phases were combined. The organic phase was dried over anhydrous sodium sulfate, filtered, and the filtrate was concentrated under reduced pressure to remove the solvent. The resulting crude product was purified by column chromatography (eluent: petroleum ether/ethyl acetate=1/0 to 24/1) to obtain compound WX004_6. .sup.1H NMR (400 MHz, CDCl.sub.3) ?: 7.52-7.45 (m, 2H), 7.41 (s, 1H), 7.20 (d, J=8.4 Hz, 4H), 6.82 (d, J=8.6 Hz, 4H), 4.17 (s, 4H), 3.78 (s, 6H), 2.44 (d, J=2.2 Hz, 6H).

Step 5: Synthesis of Compound WX004_7

[0119] tert-Butyl diethylphosphonoacetate (235.39 mg, 933.18 ?mol) was dissolved in tetrahydrofuran (5 mL) at room temperature under nitrogen atmosphere. A 1 M solution of potassium tert-butoxide in tetrahydrofuran (1.03 mL, 1.03 mmol) was added dropwise thereto at 0? C. After the addition was complete, the reaction mixture was stirred at 0? C. for 0.5 hours. A solution of WX004_6 (447.9 mg, 933.18 ?mol) in tetrahydrofuran (3 mL) was then added dropwise thereto. After the addition was complete, the reaction mixture was stirred at 20? C. for 12 hours. After the reaction was complete, the reaction mixture was poured into ice water (10 mL) to quench the reaction, extracted with ethyl acetate (15 mL?3), and the organic phases were combined. The combined organic phase was washed with saturated brine (10 mL), dried over anhydrous sodium sulfate, and filtered. The filtrate was then concentrated under reduced pressure to remove the solvent. The resulting crude product was purified by column chromatography (eluent: petroleum ether/ethyl acetate=99/1 to 97/3) to obtain target compound WX004_7. .sup.1H NMR (400 MHz, CDCl.sub.3) ?: 7.37 (d, J=8.0 Hz, 1H), 7.21 (d, J=8.6 Hz, 4H), 6.85-6.80 (m, 4H), 6.62 (dd, J=2.0, 8.0 Hz, 1H), 6.51 (d, J=1.6 Hz, 1H), 5.74 (s, 1H), 4.13 (s, 4H), 3.78 (s, 6H), 1.87 (d, J=2.4 Hz, 6H), 1.22 (s, 9H)

Step 6: Synthesis of Compound WX004_8

[0120] Platinum on carbon (100 mg, purity: 5%) was added to a dry reaction flask under argon atmosphere and wetted with tetrahydrofuran (2 mL), followed by the addition of a solution of compound WX004_7 (300 mg, 518.93 ?mol) in tetrahydrofuran (2 mL). The reaction system was replaced with hydrogen three times and then reacted at 20? C. under hydrogen (15 psi) for 12 hours. After the reaction was complete, the reaction mixture was filtered through diatomite. The filter cake was washed with tetrahydrofuran (10 mL?3). The filtrates were combined, and then concentrated under reduced pressure to remove the solvent. The resulting crude product was purified by column chromatography (eluent: petroleum ether/ethyl acetate=99/1 to 24/1, v/v) to obtain target compound WX004_8.

Step 7: Synthesis of Compound WX004_9

[0121] Compound WX004_8 (78 mg, 134.45 ?mol) was dissolved in a mixed solvent of dichloromethane (3.5 mL) and water (0.6 mL) at room temperature under nitrogen atmosphere. The reaction mixture was cooled to 0? C., and dichlorodicyanobenzoquinone (92.28 mg, 406.54 ?mol) was added thereto in batches. The reaction mixture was then stirred at 20? C. for 2 hours. After the reaction was complete, the reaction mixture was added with saturated sodium bicarbonate aqueous solution (20 mL) and stirred for 0.5 hours. The phases were then separated, and the organic phase was collected. The aqueous phase was extracted with dichloromethane (10 mL?2), and the organic phases were combined. The organic phase was dried over anhydrous sodium sulfate, filtered, and the filtrate was concentrated under reduced pressure to remove the solvent. The resulting crude product was purified by column chromatography (eluent: petroleum ether/ethyl acetate=1/0 to 19/1, v/v) to obtain target compound WX004_9.

Step 8: Synthesis of Compound WX004_10

[0122] Compound WX001_5 (55.71 mg, 208.93 ?mol) was dissolved in dichloromethane (1 mL) at room temperature under nitrogen atmosphere. The reaction mixture was cooled to 0? C., and oxalyl chloride (45.72 ?L, 522.32 ?mol) was added dropwise thereto, followed by the addition of a drop of N,N-dimethylformamide. The reaction mixture was stirred at 20? C. for 1 hour, and concentrated under reduced pressure to remove the solvent. Dichloromethane (1 mL) was added to dissolve the residue, and the reaction mixture was again concentrated under reduced pressure to obtain a crude product. The crude product was dissolved in dichloromethane (1 mL), and added dropwise to a solution of compound WX004_9 (71 mg, 208.93 ?mol) and N,N-diisopropylethylamine (67.51 mg, 522.32 ?mol) in dichloromethane (1 mL) at 0? C. The reaction mixture was then stirred at 20? C. for 12 hours. After the reaction was complete, the reaction mixture was poured into water (10 mL) to quench the reaction, extracted with dichloromethane (5 mL?3), and the organic phases were combined. The organic phase was washed with saturated brine (20 mL), dried over anhydrous sodium sulfate, filtered, and the filtrate was concentrated under reduced pressure to remove the solvent. The resulting crude product was purified by column chromatography (eluent: petroleum ether/ethyl acetate=46/1 to 17/3, v/v) to obtain target compound WX004_10. .sup.1H NMR (400 MHz, CDCl.sub.3) ?: 8.11 (d, J=1.6 Hz, 1H), 7.59 (d, J=5.6 Hz, 1H), 7.41-7.31 (m, 4H), 7.25 (dd, J=2.0, 8.4 Hz, 1H), 6.80 (d, J=8.0 Hz, 1H), 3.69-3.65 (m, 1H), 3.47-3.43 (m, 1H), 3.41-3.33 (m, 1H), 2.67-2.49 (m, 2H), 1.90-1.82 (m, 6H), 1.33 (d, J=2.4 Hz, 9H), 0.96 (d, J=7.2 Hz, 3H).

Step 9: Synthesis of Compound WX004

[0123] Compound WX004_10 (100 mg, 169.94 ?mol) was dissolved in ethyl acetate (0.5 mL) at room temperature under nitrogen atmosphere. A solution of hydrochloric acid in ethyl acetate (4 M, 2 mL) was added thereto, and the reaction system was stirred at 25? C. for 1 hour. After the reaction was complete, the reaction mixture was directly concentrated under reduced pressure to remove the solvent. The resulting residue was purified by preparative HPLC (mobile phase: acetonitrile/water; acidic system: 0.04% HCl) to obtain target compound WX004. MS-ESI m/z: 531.9 [M+H].sup.+. .sup.1H NMR (400 MHz, DMSO_d.sub.6) ?: 12.16 (s, 1H), 9.84 (d, J=3.1 Hz, 1H), 7.52-7.43 (m, 4H), 7.39-7.32 (m, 2H), 7.04-6.92 (m, 1H), 4.13 (d, J=10.8 Hz, 1H), 3.41-3.34 (m, 2H), 2.65-2.57 (m, 1H), 2.55-2.54 (m, 1H), 1.83-1.73 (m, 6H), 0.80 (d, J=6.8 Hz, 3H).

Example 5

[0124] ##STR00048##

Step 1: Synthesis of Compounds WX005 and WX006

[0125] Compound WX004 was initially separated by chiral column chromatography (column type: DAICEL CHIRALPAK IC (250 mm?30 mm, 10 ?m); mobile phase: A (CO.sub.2) and B (isopropanol, containing 0.1% diisopropylamine); gradient: B %=33% to 33%, 5 minutes) and then purified by preparative HPLC (mobile phase: acetonitrile/water; acidic system: 0.04% HCl) to obtain compounds WX005 and WX006, respectively.

[0126] SFC analysis method: column type: Chiralpak IC-3 (100?4.6 mm I.D., 3 ?m); mobile phase: A: CO.sub.2, B: [IPA (containing 0.1% IPAm)], gradient: B %: 5% to 40%, 3 minutes.

[0127] WX005 (retention time: 1.874 min): MS-ESI m/z: 532.1[M+H].sup.+. ee value: 100%. .sup.1H NMR (400 MHz, DMSO_d.sub.6) ?: 12.16 (s, 1H), 9.85 (s, 1H), 7.50-7.42 (m, 4H), 7.36 (d, J=8.4 Hz, 1H), 7.34 (d, J=2.0 Hz, 1H), 6.97 (dd, J=2.0, 8.4 Hz, 1H), 4.13 (d, J=10.8 Hz, 1H), 3.43-3.39 (m, 2H), 2.64-2.58 (m, 1H), 2.54-2.52 (m, 1H), 1.86-1.70 (m, 6H), 0.80 (d, J=6.8 Hz, 3H).

[0128] WX006 (retention time: 2.003 min): MS-ESI m/z: 532.1[M+H].sup.+. ee value: 92.44%. .sup.1H NMR (400 MHz, DMSO_d.sub.6) ?: 12.14 (s, 1H), 9.84 (s, 1H), 7.53-7.41 (m, 4H), 7.40-7.30 (m, 2H), 6.97 (d, J=8.0 Hz, 1H), 4.13 (d, J=10.8 Hz, 1H), 3.39-3.36 (m, 2H), 2.63-2.58 (m, 1H), 2.54-2.53 (m, 1H), 1.86-1.71 (m, 6H), 0.80 (d, J=6.8 Hz, 3H).

Example 7

[0129] ##STR00049##

Synthetic Route:

[0130] ##STR00050## ##STR00051##

Step 1: Synthesis of Compound WX007_1

[0131] To a dry reaction flask was added 4-fluorophenylmagnesium bromide (13.86 mL, 4.62 mmol, 1 M solution in tetrahydrofuran) at room temperature under nitrogen atmosphere. The reaction mixture was cooled to ?30? C. under nitrogen atmosphere, and a solution of compound WX004_5 (0.8 g, 4.62 mmol) in tetrahydrofuran (10 mL) was added dropwise thereto. After the addition was complete, the reaction mixture was warmed to 20? C. and stirred for 12 hours. After the reaction was complete, the reaction mixture was poured into saturated ammonium chloride aqueous solution (20 mL) to quench the reaction, and then extracted with ethyl acetate (10 mL?2). The organic phases were combined, dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated under reduced pressure to remove the solvent. The resulting residue was purified by column chromatography (eluent, petroleum ether/ethyl acetate=1/0 to 97/3, v/v) to obtain target compound WX007_1.

Step 2: Synthesis of Compound WX007_2

[0132] Fuming nitric acid (6.02 g, 95.54 mmol, 4.30 mL, purity: 95%) was placed in a dry reaction flask at room temperature under nitrogen atmosphere. The reaction mixture was then cooled to ?10? C. Compound WX007_1 (390 mg, 1.87 mmol) was slowly added thereto in batches, and the reaction mixture was stirred at 0? C. for 1 hour. After the reaction was complete, the reaction mixture was slowly poured into ice water (10 mL) to quench the reaction, and then extracted with ethyl acetate (15 mL?2). The organic phases were combined, washed with saturated sodium bicarbonate aqueous solution (10 mL), dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated under reduced pressure to remove the solvent. The resulting residue was purified by column chromatography (eluent, petroleum ether/ethyl acetate=1/0 to 97/3, v/v) to obtain target compound WX007_2.

Step 3: Synthesis of Compound WX007_3

[0133] tert-Butyl diethylphosphonoacetate (318.79 mg, 1.26 mmol) was dissolved in tetrahydrofuran (3 mL) at room temperature under nitrogen atmosphere. The reaction mixture was cooled to 0? C. under nitrogen atmosphere, and a 1 M solution of potassium tert-butoxide in tetrahydrofuran (1.39 mL, 1.39 mmol) was added dropwise thereto. After the addition was complete, the reaction mixture was stirred for 0.5 hours. A solution of compound WX007_2 (320 mg, 1.26 mmol) in tetrahydrofuran (3 mL) was then added dropwise thereto, and the reaction mixture was stirred at 20? C. for 12 hours. After the reaction was complete, the reaction mixture was poured into water (20 mL), and extracted with ethyl acetate (10 mL?3). The organic phase was washed with saturated brine (30 mL), dried over anhydrous sodium sulfate, filtered, and the filtrate was concentrated under reduced pressure to remove the solvent. The resulting residue was purified by column chromatography (eluent, petroleum ether/ethyl acetate=1/0 to 97/3, v/v) to obtain target compound WX007_3. .sup.1H NMR (400 MHz, CDCl.sub.3) ?: 7.76 (dd, J=2.0, 7.0 Hz, 1H), 7.34-7.30 (m, 1H), 7.27-7.22 (m, 1H), 5.91 (s, 1H), 2.14 (d, J=2.4 Hz, 6H), 1.27 (s, 9H).

Step 4: Synthesis of Compound WX007_4

[0134] Platinum on carbon (150.00 mg, purity: 5%) was placed in a reaction flask under argon atmosphere and wetted with tetrahydrofuran (3 mL). A solution of compound WX007_3 (300 mg, 853.86 ?mol) in tetrahydrofuran (2 mL) was then added thereto. The reaction mixture was replaced with hydrogen three times, and reacted at 20? C. under hydrogen atmosphere of 15 psi for 12 hours. After the reaction was complete, the reaction mixture was filtered through diatomite. The filter cake was washed with tetrahydrofuran (5 mL). The filtrates were combined and concentrated under reduced pressure to remove the solvent. Thus target compound WX007_4 was obtained, and the crude product was directly used in the next step.

Step 5: Synthesis of Compound WX007_5

[0135] Compound WX001_5 (340 mg, 1.28 mmol) was dissolved in dichloromethane (4 mL) at room temperature under nitrogen atmosphere. The reaction mixture was cooled to 0? C., and oxalyl chloride (404.62 mg, 3.19 mmol, 279.05 ?L) was added dropwise thereto. A drop of N,N-dimethylformamide was then added thereto, and the reaction mixture was stirred at 20? C. for 1 hour. After the reaction was complete, the reaction mixture was directly concentrated under reduced pressure to remove the solvent. The residue was dissolved in dichloromethane (1 mL). To a solution of compound WX007_4 (210 mg, 649.40 ?mol) and N,N-diisopropylethylamine (209.82 mg, 1.62 mmol, 282.78 ?L) in dichloromethane (2 mL) was added dropwise the prepared acyl chloride at 0? C. The reaction mixture was then stirred at 20? C. for 12 hours. After the reaction was complete, the reaction mixture was concentrated under reduced pressure to remove the solvent. The resulting residue was purified by column chromatography (eluent, petroleum ether/ethyl acetate=49/1 to 17/3, v/v) to obtain target compound WX007_5.

Step 6: Synthesis of Compound WX007

[0136] Compound WX007_5 (70 mg, 122.38 ?mol) was dissolved in a solution of hydrochloric acid in ethyl acetate (4 M, 2 mL) at room temperature under nitrogen atmosphere. The reaction system was stirred at 20? C. for 2 hours. After the reaction was complete, the reaction mixture was directly concentrated under reduced pressure to remove the solvent. The residue was purified by preparative HPLC (mobile phase: acetonitrile/water; acidic system: 0.04% HCl) to obtain target compound WX007. MS-ESI m/z: 515.9 [M+H].sup.+. .sup.1H NMR (400 MHz, DMSO_d.sub.6) ?: 12.12 (s, 1H), 10.06 (s, 1H), 7.58-7.61 (m, 1H), 7.49-7.42 (m, 4H), 7.18-7.10 (m, 1H), 6.96-6.88 (m, 1H), 4.11 (dd, J=2.6, 10.6 Hz, 1H), 3.37 (s, 2H), 2.63-2.57 (m, 1H), 2.52-2.51 (m, 1H), 1.82-1.72 (m, 6H), 0.79 (d, J=7.0 Hz, 3H).

Example 8

[0137] ##STR00052##

Step 1: Synthesis of Compounds WX008 and WX009

[0138] Compound WX007 was initially separated by chiral column chromatography (column type: DAICEL CHIRALPAK IC (250 mm?30 mm, 10 ?m); mobile phase: A (CO.sub.2) and B (isopropanol containing 0.1% ammonia); gradient: B %=20% to 20%, 4 minutes) and then purified by preparative HPLC (mobile phase: acetonitrile/water; acidic system: 0.04% HCl) to obtain compounds WX008 and WX009, respectively.

[0139] SFC analysis method: column type: Chiralpak IC-3, 50?4.6 mm I.D., 3 ?m; mobile phase: A: CO.sub.2, B: [IPA (containing 0.1% IPAm)], gradient: B %: 5% to 50%, 3 minutes.

[0140] WX008 (retention time: 0.966 min): MS-ESI m/z: 515.9 [M+H].sup.+. ee value: 100%.

[0141] .sup.1H NMR (400 MHz, DMSO_d.sub.6) ?: 12.13 (s, 1H), 10.07 (s, 1H), 7.60 (dd, J=2.2, 7.4 Hz, 1H), 7.50-7.43 (m, 4H), 7.15 (dd, J=8.4, 10.8 Hz, 1H), 6.95-6.89 (m, 1H), 4.12 (d, J=10.8 Hz, 1H), 3.42-3.36 (m, 2H), 2.64-2.57 (m, 1H), 2.55-2.52 (m, 1H), 1.85-1.72 (m, 6H), 0.80 (d, J=7.0 Hz, 3H).

[0142] WX009 (retention time: 1.021 min): MS-ESI m/z: 516.2 [M+H].sup.+. ee value: 90.3%. .sup.1H NMR (400 MHz, DMSO_d.sub.6) ?: 12.13 (s, 1H), 10.07 (s, 1H), 7.61 (dd, J=2.2, 7.4 Hz, 1H), 7.49-7.44 (m, 4H), 7.15 (dd, J=8.4, 10.8 Hz, 1H), 6.94-6.91 (m, 1H), 4.12 (d, J=10.8 Hz, 1H), 3.42-3.38 (m, 2H), 2.65-2.57 (m, 1H), 2.64-2.57 (m, 1H), 1.92-1.60 (m, 6H), 0.79 (d, J=7.0 Hz, 3H).

Example 10

[0143] ##STR00053##

Synthetic Route:

[0144] ##STR00054## ##STR00055##

Step 1: Synthesis of Compound WX010_1

[0145] To a dry reaction flask was added 4-methylphenylmagnesium chloride (2.89 mL, 5.77 mmol, 2 M solution in tetrahydrofuran) at room temperature under nitrogen atmosphere. The reaction mixture was cooled to ?30? C. under nitrogen atmosphere, and a solution of compound WX004_5 (0.4 g, 2.31 mmol) in tetrahydrofuran (5 mL) was added dropwise thereto. After the addition was complete, the reaction mixture was warmed to 20? C. and stirred for 12 hours. After the reaction was complete, the reaction mixture was poured into saturated ammonium chloride aqueous solution (20 mL) to quench the reaction, and then extracted with ethyl acetate (10 mL?2). The organic phases were combined, dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated under reduced pressure to remove the solvent. The resulting residue was purified by column chromatography (eluent, petroleum ether/ethyl acetate=1/0 to 97/3, v/v) to obtain target compound WX010_1.

Step 2: Synthesis of Compound WX010_2

[0146] Fuming nitric acid (1.12 g, 17.77 mmol, 0.80 mL, purity: 95%) was placed in a dry reaction flask at room temperature under nitrogen atmosphere. The reaction mixture was then cooled to ?10? C. Compound WX010_1 (120 mg, 587.55 ?mol) was slowly added thereto in batches, and the reaction mixture was stirred at 0? C. for 1 hour. After the reaction was complete, the reaction mixture was slowly poured into ice water (10 mL) to quench the reaction, and then extracted with ethyl acetate (5 mL?2). The organic phases were combined, washed with saturated sodium bicarbonate aqueous solution (10 mL), dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated under reduced pressure to remove the solvent. The residue was purified by column chromatography (eluent, petroleum ether/ethyl acetate=1/0 to 97/3, v/v) to obtain target compound WX010_2. .sup.1H NMR (400 MHz, CDCl.sub.3) ?: 8.53 (d, J=1.8 Hz, 1H), 8.06 (dd, J=1.8, 8.0 Hz, 1H), 7.48 (d, J=8.0 Hz, 1H), 2.69 (s, 3H), 2.63 (d, J=2.4 Hz, 6H).

Step 3: Synthesis of Compound WX010_3

[0147] tert-Butyl diethylphosphonoacetate (86.03 mg, 341.04 ?mol) was dissolved in tetrahydrofuran (1 mL) at room temperature under nitrogen atmosphere. The reaction mixture was cooled to 0? C. under nitrogen atmosphere, and a 1 M solution of potassium tert-butoxide in tetrahydrofuran (375.14 ?L, 375.14 ?mol) was added dropwise thereto. After the addition was complete, the reaction mixture was stirred for 0.5 hours. A solution of compound WX010_2 (85 mg, 341.04 ?mol) in tetrahydrofuran (1 mL) was then added dropwise thereto, and the reaction mixture was stirred at 20? C. for 12 hours. After the reaction was complete, the reaction mixture was poured into water (20 mL), and extracted with ethyl acetate (10 mL?3). The organic phases were combined, washed with saturated brine (30 mL), dried over anhydrous sodium sulfate, filtered, and the filtrate was concentrated under reduced pressure to remove the solvent. The residue was purified by column chromatography (eluent, petroleum ether/ethyl acetate=1/0 to 97/3, v/v) to obtain target compound WX010_3.

Step 4: Synthesis of Compound WX010_4

[0148] Platinum on carbon (40.00 mg, purity: 5%) was placed in a reaction flask under argon atmosphere and wetted with tetrahydrofuran (3 mL). A solution of compound WX010_3 (80 mg, 230.30 ?mol) in tetrahydrofuran (1 mL) was then added thereto. The reaction mixture was replaced with hydrogen three times, and stirred at 20? C. under hydrogen atmosphere of 15 psi for 12 hours. After the reaction was complete, the reaction mixture was filtered through diatomite. The filter cake was washed with tetrahydrofuran (5 mL). The filtrates were combined and concentrated under reduced pressure to remove the solvent. Thus compound WX010_4 was obtained, and the crude product was directly used in the next step.

Step 5: Synthesis of Compound WX010_5

[0149] Compound WX001_5 (70.12 mg, 262.98 ?mol) was dissolved in dichloromethane (4 mL) at room temperature under nitrogen atmosphere, and the reaction mixture was cooled to 0? C. To the reaction mixture was added dropwise oxalyl chloride (69.54 mg, 547.88 ?mol, 47.96 ?L), followed by the addition of a drop of N,N-dimethylformamide. The reaction mixture was stirred at 20? C. for 1 hour. After the reaction was complete, the reaction mixture was directly concentrated under reduced pressure to remove the solvent. The residue was dissolved in dichloromethane (1 mL). To a solution of compound WX010_4 (70 mg, 219.15 ?mol) and N,N-diisopropylethylamine (70.81 mg, 547.88 ?mol, 95.43 ?L) in dichloromethane (2 mL) was added dropwise a prepared solution of acyl chloride in dichloromethane at 0? C. The reaction mixture was then stirred at 20? C. for 12 hours. After the reaction was complete, the reaction mixture was concentrated under reduced pressure to remove the solvent. The resulting residue was purified by column chromatography (eluent, petroleum ether/ethyl acetate=49/1 to 17/3, v/v) to obtain target compound WX010_5. .sup.1H NMR (400 MHz, CDCl.sub.3) ?: 7.45 (s, 1H), 7.42-7.28 (m, 4H), 7.05 (d, J=7.2 Hz, 1H), 6.90 (s, 1H), 6.82 (d, J=7.6 Hz, 1H), 3.65 (dd, J=4.8, 8.4 Hz, 1H), 3.42 (t, J=7.8 Hz, 2H), 2.63-2.49 (m, 2H), 2.03 (d, J=8.2 Hz, 3H), 1.84 (s, 6H), 1.33 (s, 9H), 0.95 (d, J=7.2 Hz, 3H).

Step 6: Synthesis of Compound WX010

[0150] Compound WX010_5 (118 mg, 207.73 ?mol) was dissolved in a solution of hydrochloric acid in ethyl acetate (4 M, 2.95 mL) at room temperature under nitrogen atmosphere. The reaction system was stirred at 20? C. for 2 hours. After the reaction was complete, the reaction mixture was directly concentrated under reduced pressure to remove the solvent. The residue was purified by preparative HPLC (mobile phase: acetonitrile/water; acidic system: 0.04% HCl) to obtain target compound WX010. MS-ESI m/z: 512.0 [M+H].sup.+. .sup.1H NMR (400 MHz, DMSO_d.sub.6) ?: 12.10 (s, 1H), 9.60 (d, J=4.0 Hz, 1H), 7.46 (d, J=1.3 Hz, 4H), 7.11-7.04 (m, 1H), 7.04-6.96 (m, 1H), 6.89-6.82 (m, 1H), 3.97 (d, J=10.8 Hz, 1H), 3.45-3.35 (m, 2H), 2.62-2.55 (m, 1H), 2.52 (d, J=1.9 Hz, 1H), 1.96 (d, J=15.4 Hz, 3H), 1.82-1.72 (m, 6H), 0.81 (d, J=7.0 Hz, 3H).

Example 11

[0151] ##STR00056##

Step 1: Synthesis of Compounds WX011 and WX012

[0152] Compound WX010 was initially separated by chiral column chromatography (column type: DAICEL CHIRALCEL OX (250 mm?30 mm, 10 ?m); mobile phase: A (CO.sub.2) and B (isopropanol containing 0.1% ammonia); gradient: B %=30% to 30%, 7 minutes) and then purified by preparative HPLC (mobile phase: acetonitrile/water; acidic system: 0.04% HCl) to obtain compounds WX011 and WX012, respectively.

[0153] SFC analysis method: column type: Chiralcel OX-3, 50?4.6 mm I.D., 3 ?m; mobile phase: A: CO.sub.2, B: [IPA (containing 0.1% IPAm)], gradient: B %: 5% to 50%, 3 minutes.

[0154] WX011 (retention time: 1.119 min): MS-ESI m/z: 512.2 [M+H].sup.+. ee value: 100%. .sup.1H NMR (400 MHz, DMSO_d.sub.6) ?: 12.10 (s, 1H), 9.61 (s, 1H), 7.46 (s, 4H), 7.08 (d, J=8.0 Hz, 1H), 7.01 (d, J=1.6 Hz, 1H), 6.86 (dd, J=1.6, 7.8 Hz, 1H), 3.97 (d, J=10.6 Hz, 1H), 3.44-3.34 (m, 2H), 2.62-2.56 (m, 1H), 2.52 (d, J=1.9 Hz, 1H), 1.95 (s, 3H), 1.83-1.73 (m, 6H), 0.81 (d, J=7.0 Hz, 3H).

[0155] WX012 (retention time: 1.208 min): MS-ESI m/z: 512.2 [M+H].sup.+. ee value: 100%. .sup.1H NMR (400 MHz, DMSO_d.sub.6) ?: 12.09 (s, 1H), 9.60 (s, 1H), 7.46 (s, 4H), 7.08 (d, J=8.0 Hz, 1H), 6.99 (s, 1H), 6.86 (d, J=7.8 Hz, 1H), 3.97 (d, J=10.8 Hz, 1H), 3.46-3.33 (m, 2H), 2.62-2.54 (m, 1H), 2.52 (s, 1H), 1.98 (s, 3H), 1.82-1.72 (m, 6H), 0.81 (d, J=7.0 Hz, 3H).

Example 13

[0156] ##STR00057##

Synthetic Route:

[0157] ##STR00058## ##STR00059##

Step 1: Synthesis of Compound WX013_2

[0158] WX013_1 (8.0 g, 47.01 mmol) and methoxymethylamine hydrochloride (5.04 g, 51.72 mmol) were dissolved in ethyl acetate (150 mL) at room temperature under nitrogen atmosphere. N-methylmorpholine (14.27 g, 141.04 mmol, 15.51 mL) was added dropwise thereto, followed by a dropwise addition of 1-propylphosphonic acid cyclic anhydride (44.88 g, 70.52 mmol, 41.94 mL, 50% solution in ethyl acetate). The reaction mixture was stirred at 20? C. for 12 hours. After the reaction was complete, the reaction mixture was poured into water (100 mL) to quench the reaction. The phases were separated, and the organic phase was collected. The aqueous phase was extracted with ethyl acetate (200 mL?2), and the organic phases were combined. The organic phase was washed with saturated brine (200 mL?2), dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated under reduced pressure to remove the solvent. The resulting residue was purified by column chromatography (eluent: petroleum ether/ethyl acetate=1/0 to 1/1, v/v) to obtain compound WX013_2. .sup.1H NMR (400 MHz, DMSO_d.sub.6) ?: 3.65 (s, 3H), 3.61 (s, 3H), 3.08 (s, 3H), 2.26 (s, 6H).

Step 2: Synthesis of Compound WX013_3

[0159] WX013_2 (2.7 g, 12.66 mmol) was dissolved in anhydrous tetrahydrofuran (40 mL) at room temperature under nitrogen atmosphere. The reaction mixture was cooled to 0? C. under nitrogen atmosphere. A 3 M solution of phenylmagnesium bromide in tetrahydrofuran (6.33 mL, 18.99 mmol) was then slowly added dropwise thereto. After the addition was complete, the reaction mixture was stirred at 0? C. for 0.5 hours, then warmed to 20? C., and stirred for another 12 hours. After the reaction was complete, the reaction mixture was slowly poured into saturated ammonium chloride solution (100 mL) to quench the reaction, extracted with ethyl acetate (50 mL?2), and the organic phases were combined. The organic phase was washed with saturated brine (50 mL?2), dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated under reduced pressure to remove the solvent. The resulting residue was purified by column chromatography (eluent: petroleum ether/ethyl acetate=1/0 to 9/1, v/v) to obtain compound WX013_3. .sup.1H NMR (400 MHz, CDCl.sub.3) ?: 8.00-7.96 (m, 2H), 7.61-7.55 (m, 1H), 7.51-7.43 (m, 2H), 3.73 (s, 3H), 2.56 (s, 6H).

Step 3: Synthesis of Compound WX013_4

[0160] WX013_3 (2.1 g, 9.12 mmol) was dissolved in anhydrous dichloromethane (25 mL) at room temperature under nitrogen atmosphere. The reaction mixture was cooled to 0? C. under nitrogen atmosphere. m-Chloroperoxybenzoic acid (2.78 g, 13.68 mmol, purity: 85%) was then slowly added thereto in batches. After the addition was complete, the reaction mixture was stirred at 20? C. for 2 hours, then cooled to 0? C., and an additional amount of m-chloroperoxybenzoic acid (2.78 g, 13.68 mmol, purity: 85%) was added thereto. The reaction mixture was subsequently warmed to 20? C., and stirred for another 12 hours. After the reaction was complete, the reaction mixture was slowly poured into saturated sodium sulfite solution (50 mL) to quench the reaction, and then extracted with dichloromethane (20 mL?2). The organic phases were combined, dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated under reduced pressure to remove the solvent. The resulting residue was purified by column chromatography (eluent: petroleum ether/ethyl acetate=19/1 to 17/3, v/v) to obtain compound WX013_4.

Step 4: Synthesis of Compound WX013_5

[0161] WX013_4 (1.0 g, 4.06 mmol) was dissolved in a solution of hydrochloric acid in methanol (4 M, 20.00 mL) at room temperature under nitrogen atmosphere. The reaction mixture was heated to 50? C., and stirred for 4 hours. After the reaction was complete, the reaction mixture was directly concentrated under reduced pressure to remove the solvent. The resulting residue was purified by column chromatography (eluent: petroleum ether/ethyl acetate=19/1 to 7/3, v/v) to obtain compound WX013_5. .sup.1H NMR (400 MHz, CDCl.sub.3) ?: 3.69 (s, 3H), 2.85 (s, 1H), 2.22 (s, 6H).

Step 5: Synthesis of Compound WX013_6

[0162] WX013_5 (374 mg, 2.63 mmol) was dissolved in anhydrous dichloromethane (20 mL) at room temperature under nitrogen atmosphere. 1,8-Bis(dimethylamino)naphthalene (2.31 g, 10.79 mmol) and trimethyloxonium tetrafluoroborate (1.21 g, 8.16 mmol) were then added thereto respectively. After the addition was complete, the reaction mixture was stirred at 20? C. for 2 hours. After the reaction was complete, the reaction mixture was filtered. The filtrate was sequentially washed with 2 M dilute hydrochloric acid (20 mL) and saturated brine (20 mL), dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to remove the solvent. The resulting residue was purified by column chromatography (eluent: petroleum ether/ethyl acetate=1/0 to 9/1, v/v) to obtain compound WX013_6. .sup.1H NMR (400 MHz, CDCl.sub.3) ?: 3.70 (s, 3H), 3.31 (s, 3H), 2.18 (s, 6H).

Step 6: Synthesis of Compound WX013_7

[0163] WX013_6 (322 mg, 2.06 mmol) was dissolved in a mixed solution of tetrahydrofuran (6 mL), methanol (2 mL), and water (2 mL) at room temperature under nitrogen atmosphere. Lithium hydroxide monohydrate (173.04 mg, 4.12 mmol) was then added thereto. After the addition was complete, the reaction mixture was stirred at 20? C. for 12 hours. After the reaction was complete, the reaction mixture was diluted with water (10 mL). The pH of the reaction mixture was adjusted to approximately 2 to 3 using 1 M dilute hydrochloric acid. The reaction mixture was diluted with ethyl acetate (10 mL), and the phases were separated. The organic phase was collected, and the aqueous phase was extracted with ethyl acetate (10 mL?2). The organic phases were then combined. The organic phase was washed with saturated brine (20 mL), dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated under reduced pressure to remove the solvent, and compound WX013_7 was obtained. The crude product was directly used in the next step.

Step 7: Synthesis of Compound WX013_8

[0164] WX013_7 (265 mg, 1.86 mmol) and methoxymethylamine hydrochloride (218.21 mg, 2.24 mmol) were dissolved in ethyl acetate (3 mL) at room temperature under nitrogen atmosphere. N-methylmorpholine (754.23 mg, 7.46 mmol, 819.82 ?L) was added dropwise thereto, followed by a dropwise addition of 1-propylphosphonic acid cyclic anhydride (1.78 g, 2.80 mmol, 1.66 mL, 50% solution in ethyl acetate). The reaction mixture was stirred at 20? C. for 12 hours. After the reaction was complete, the reaction mixture was poured into water (10 mL) to quench the reaction. The phases were separated, and the organic phase was collected. The aqueous phase was extracted with ethyl acetate (10 mL?2), and the organic phases were combined. The organic phase was washed with saturated brine (20 mL), dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated under reduced pressure to remove the solvent. The resulting residue was purified by column chromatography (eluent: petroleum ether/ethyl acetate=19/1 to 17/3, v/v) to obtain compound WX013_8. .sup.1H NMR (400 MHz, CDCl.sub.3) ?: 3.67 (s, 3H), 3.32 (s, 3H), 3.20 (s, 3H), 2.23 (s, 6H).

Step 8: Synthesis of Compound WX013_9

[0165] To a dry reaction flask was added 4-chlorophenylmagnesium bromide (4.54 mL, 4.54 mmol, 1 M solution in tetrahydrofuran) at room temperature under nitrogen atmosphere. The reaction mixture was cooled to ?30? C. under nitrogen atmosphere, and a solution of compound WX013_8 (280 mg, 1.51 mmol) in tetrahydrofuran (5 mL) was added dropwise thereto. After the addition was complete, the reaction mixture was warmed to 20? C. and stirred for 2 hours. After the reaction was complete, the reaction mixture was poured into saturated ammonium chloride aqueous solution (10 mL) to quench the reaction, and then extracted with ethyl acetate (10 mL?2). The organic phases were combined, dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated under reduced pressure to remove the solvent. The residue was purified by column chromatography (eluent, petroleum ether/ethyl acetate=1/0 to 19/1, v/v) to obtain target compound WX013_9.

Step 9: Synthesis of Compound WX013_10

[0166] Fuming nitric acid (3 g, 47.61 mmol, 2.14 mL, purity: 95%) was placed in a dry reaction flask at room temperature under nitrogen atmosphere. The reaction mixture was then cooled to ?10? C. Compound WX013_9 (160 mg, 675.98 ?mol) was slowly added thereto in batches, and the reaction mixture was stirred at 0? C. for 2 hours. After the reaction was complete, the reaction mixture was slowly poured into ice water (10 mL) to quench the reaction, and then extracted with ethyl acetate (5 mL?2). The organic phases were combined, washed with saturated brine (10 mL), dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated under reduced pressure to remove the solvent. The residue was purified by column chromatography (eluent, petroleum ether/ethyl acetate=1/0 to 9/1, v/v) to obtain target compound WX013_10. .sup.1H NMR (400 MHz, CDCl.sub.3) ?: 8.45 (d, J=2.0 Hz, 1H), 8.09 (dd, J=2.0, 8.4 Hz, 1H), 7.67 (d, J=8.4 Hz, 1H), 3.37 (s, 3H), 2.44 (s, 6H).

Step 10: Synthesis of Compound WX013_11

[0167] tert-Butyl diethylphosphonoacetate (148.65 mg, 589.30 ?mol) was dissolved in tetrahydrofuran (2 mL) at room temperature under nitrogen atmosphere. The reaction mixture was cooled to 0? C. under nitrogen atmosphere, and a 1 M solution of potassium tert-butoxide in tetrahydrofuran (648.23 ?L, 648.23 ?mol) was added dropwise thereto. After the addition was complete, the reaction mixture was stirred for 0.5 hours. A solution of compound WX013_10 (166 mg, 589.30 ?mol) in tetrahydrofuran (1 mL) was then added thereto, and the reaction mixture was stirred at 20? C. for 2 hours. After the reaction was complete, the reaction mixture was poured into water (10 mL), and extracted with ethyl acetate (10 mL?3). The organic phase was washed with saturated brine (30 mL), dried over anhydrous sodium sulfate, filtered, and the filtrate was concentrated under reduced pressure to remove the solvent. The residue was purified by column chromatography (eluent, petroleum ether/ethyl acetate=1/0 to 20/1, v/v) to obtain target compound WX013_11. .sup.1H NMR (400 MHz, CDCl.sub.3) ?: 7.61 (d, J=2.0 Hz, 1H), 7.54 (d, J=8.3 Hz, 1H), 7.25-7.21 (m, 1H), 5.91 (s, 1H), 3.29 (s, 3H), 1.97 (s, 6H), 1.28 (s, 9H).

Step 11: Synthesis of Compound WX013_12

[0168] Platinum on carbon (80.00 mg, purity: 5%) was placed in a reaction flask under argon atmosphere and wetted with tetrahydrofuran (1.5 mL). A solution of compound WX013_11 (160 mg, 421.24 ?mol) in tetrahydrofuran (1.5 mL) was then added thereto. The reaction mixture was replaced with hydrogen three times, and stirred at 20? C. under hydrogen atmosphere of 15 psi for 16 hours. After the reaction was complete, the reaction mixture was filtered through diatomite. The filter cake was washed with tetrahydrofuran (5 mL). The filtrates were combined and concentrated under reduced pressure to remove the solvent. The residue was purified by column chromatography (eluent, petroleum ether/ethyl acetate=1/0 to 9/1, v/v) to obtain target compound WX013_12.

Step 12: Synthesis of Compound WX013_13

[0169] Compound WX001_5 (97.76 mg, 366.62 ?mol) was dissolved in dichloromethane (1 mL) at room temperature under nitrogen atmosphere, and the reaction mixture was cooled to 0? C. To the reaction mixture was added dropwise oxalyl chloride (77.56 mg, 611.03 ?mol, 53.49 ?L), followed by the addition of a drop of N,N-dimethylformamide. The reaction mixture was stirred at 20? C. for 1 hour. After the reaction was complete, the reaction mixture was directly concentrated under reduced pressure to remove the solvent. The residue was dissolved in dichloromethane (1 mL). To a solution of compound WX013_12 (86 mg, 244.41 ?mol) and N,N-diisopropylethylamine (78.97 mg, 611.03 ?mol, 106.43 ?L) in dichloromethane (1 mL) was added dropwise the prepared acyl chloride at 0? C. The reaction mixture was then stirred at 20? C. for 12 hours. After the reaction was complete, the reaction mixture was concentrated under reduced pressure to remove the solvent. The resulting residue was purified by column chromatography (eluent, petroleum ether/ethyl acetate=19/1 to 9/1, v/v) to obtain target compound WX013_13. .sup.1H NMR (400 MHz, CDCl.sub.3) ?: 8.11 (s, 1H), 7.58 (d, J=5.6 Hz, 1H), 7.42-7.30 (m, 4H), 7.24 (dd, J=1.4, 8.2 Hz, 1H), 6.81 (d, J=8.4 Hz, 1H), 3.71-3.65 (m, 1H), 3.39 (t, J=7.5 Hz, 2H), 3.23 (s, 3H), 2.66-2.47 (m, 2H), 1.71-1.63 (m, 6H), 1.32 (d, J=2.4 Hz, 9H), 0.96 (d, J=7.2 Hz, 3H).

Step 13: Synthesis of Compound WX013

[0170] Compound WX013_13 (105 mg, 174.86 ?mol) was dissolved in a solution of hydrochloric acid in ethyl acetate (4 M, 4 mL) at room temperature under nitrogen atmosphere. The reaction system was stirred at 20? C. for 2 hours. After the reaction was complete, the reaction mixture was directly concentrated under reduced pressure to remove the solvent. The residue was purified by preparative HPLC (mobile phase: acetonitrile/water; acidic system: 0.04% HCl) to obtain target compound WX013. MS-ESI m/z: 544.2 [M+H].sup.+. .sup.1H NMR (400 MHz, DMSO_d.sub.6) ?: 11.87 (s, 1H), 9.83 (d, J=2.8 Hz, 1H), 7.51-7.41 (m, 4H), 7.38-7.30 (m, 2H), 6.96 (dd, J=2.1, 8.3 Hz, 1H), 4.13 (d, J=10.5 Hz, 1H), 3.30-3.24 (m, 2H), 3.10 (d, J=1.3 Hz, 3H), 2.62-2.55 (m, 1H), 2.52 (d, J=1.9 Hz, 1H), 1.60-1.49 (m, 6H), 0.80 (d, J=7.0 Hz, 3H).

Example 14

[0171] ##STR00060##

Step 1: Synthesis of Compounds WX014 and WX015

[0172] Compound WX013 was initially separated by chiral column chromatography (column type: DAICEL CHIRALPAK IC (250 mm?30 mm, 10 ?m); mobile phase: A (CO.sub.2) and B (isopropanol containing 0.1% ammonia); gradient: B %=30% to 30%, 10 minutes) and then separated by preparative HPLC (mobile phase: acetonitrile/water; acidic system: 0.04% HCl) to obtain compounds WX014 and WX015, respectively.

[0173] SFC analysis method: column type: (S,S)-WHELK-01, 50?4.6 mm I.D., 3.0 ?m; mobile phase: A: hexane, B: [ethanol (containing 0.1% TFA)], gradient: B %: 5% to 50%, 6 minutes.

[0174] WX014 (retention time: 3.051 min): MS-ESI m/z: 544.2 [M+H].sup.+. ee value: 100%. .sup.1H NMR (400 MHz, DMSO_d.sub.6) ?: 12.11 (s, 1H), 9.83 (s, 1H), 7.50-7.42 (m, 4H), 7.37-7.31 (m, 2H), 6.96 (dd, J=2.0, 8.3 Hz, 1H), 4.13 (d, J=10.5 Hz, 1H), 3.30-3.23 (m, 2H), 3.10 (s, 3H), 2.62-2.55 (m, 1H), 2.47-2.44 (m, 1H), 1.64-1.43 (m, 6H), 0.81 (d, J=7.2 Hz, 3H).

[0175] WX015 (retention time: 3.194 min): MS-ESI m/z: 544.2 [M+H].sup.+. ee value: 100%. .sup.1H NMR (400 MHz, DMSO_d.sub.6) ?: 12.11 (s, 1H), 9.82 (s, 1H), 7.52-7.42 (m, 4H), 7.38-7.29 (m, 2H), 6.95 (dd, J=2.1, 8.3 Hz, 1H), 4.13 (d, J=10.7 Hz, 1H), 3.30-3.24 (m, 2H), 3.09 (s, 3H), 2.61-2.55 (m, 1H), 2.53-2.52 (m, 1H), 1.60-1.48 (m, 6H), 0.80 (d, J=7.0 Hz, 3H).

Example 16

[0176] ##STR00061##

Synthetic Route:

[0177] ##STR00062## ##STR00063## ##STR00064## ##STR00065## ##STR00066##

Step 1: Synthesis of Compound WX016_2

[0178] WX016_1 (1.0 g, 9.79 mmol) was dissolved in dichloromethane (10 mL) at room temperature under nitrogen atmosphere, and the reaction mixture was cooled to 0? C. under nitrogen atmosphere. Dess-Martin periodinane (4.98 g, 11.75 mmol) was added thereto in batches. The reaction mixture was warmed to 20? C. and stirred for 2 hours. After the reaction was complete, the reaction mixture was added with saturated sodium bicarbonate solution (20 mL) and stirred for 0.5 hours. The phases were then separated, and the organic phase was collected. The aqueous phase was extracted with dichloromethane (10 mL?2). The organic phases were combined, dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated under reduced pressure to obtain target compound WX016_2.

Step 2: Synthesis of Compound WX016_3

[0179] tert-Butyl diethylphosphonoacetate (1.01 g, 4.00 mmol) was dissolved in tetrahydrofuran (10 mL) at room temperature under nitrogen atmosphere. The reaction mixture was cooled to 0? C. under nitrogen atmosphere, and a 1 M solution of potassium tert-butoxide in tetrahydrofuran (4.39 mL, 4.39 mmol) was added dropwise thereto. After the addition was complete, the reaction mixture was stirred for 0.5 hours. Compound WX016_2 (400 mg, 4.00 mmol) was then added dropwise thereto. The reaction mixture was stirred at 20? C. for 2 hours. After the reaction was complete, the reaction mixture was poured into water (20 mL), and extracted with ethyl acetate (10 mL?3). The organic phase was washed with saturated brine (30 mL), dried over anhydrous sodium sulfate, filtered, and the filtrate was concentrated under reduced pressure to remove the solvent. The residue was purified by column chromatography (eluent, petroleum ether/ethyl acetate=1/0 to 9/1, v/v) to obtain target compound WX016_3. .sup.1H NMR (400 MHz, CDCl.sub.3) ?:6.76-6.85 (m, 1H), 5.89-5.97 (m, 1H), 4.45-4.54 (m, 1H), 3.90-3.98 (m, 1H), 3.80-3.88 (m, 1H), 2.05-2.16 (m, 1H), 1.88-1.97 (m, 2H), 1.66-1.74 (m, 1H), 1.49 (s, 9H).

Step 3: Synthesis of Compounds WX016_4 and WX016_5

[0180] Compound WX016_3 was initially separated by chiral column chromatography (column type: REGIS(S,S)WHELK-01, (250 mm?25 mm, 10 ?m); mobile phase: A (CO.sub.2) and B (isopropanol containing 0.1% ammonia); gradient: B %=30% to 30%, 10 minutes) to obtain compounds WX016_4 and WX016_5, respectively.

[0181] SFC analysis method: column type: WK, 100?4.6 mm I.D., 3 ?m; mobile phase: A: CO.sub.2, B: [IPA (containing 0.1% IPAm)], gradient: B %: 10% to 50%, 4 minutes.

[0182] WX016_4 (retention time: 0.635 min): ee value: 100%. .sup.1H NMR (400 MHz, CDCl.sub.3) ?: 6.81 (dd, J=5.2, 15.6 Hz, 1H), 5.93 (dd, J=1.4, 15.4 Hz, 1H), 4.52-4.46 (m, 1H), 3.98-3.90 (m, 1H), 3.88-3.80 (m, 1H), 2.17-2.07 (m, 1H), 1.98-1.91 (m, 2H), 1.75-1.65 (m, 1H), 1.49 (s, 9H)

[0183] WX016_5 (retention time: 0.804 min): ee value: 100%. .sup.1H NMR (400 MHz, CDCl.sub.3) ?: 6.81 (dd, J=5.2, 15.6 Hz, 1H), 5.93 (dd, J=1.4, 15.6 Hz, 1H), 4.52-4.46 (m, 1H), 3.97-3.90 (m, 1H), 3.87-3.80 (m, 1H), 2.18-2.08 (m, 1H), 1.99-1.90 (m, 2H), 1.74-1.66 (m, 1H), 1.49 (s, 9H).

Step 4: Synthesis of Compound WX016_6

[0184] Chloro(1,5-cyclooctadiene)rhodium(I) dimer (15.54 mg, 31.52 ?mol) and 2,2-bis(diphenylphosphino)-1,1-binaphthalene (4.51 mg, 7.25 ?mol) were dissolved in tetrahydrofuran (3 mL) at room temperature under nitrogen atmosphere. The reaction mixture was stirred at 20? C. for 15 minutes. 3-Amino-4-chlorophenylboronic acid pinacol ester (319.70 mg, 1.26 mmol) was dissolved in a mixed solvent of isopropanol (1.5 mL) and tetrahydrofuran (3 mL) at room temperature under nitrogen atmosphere. To the reaction mixture were added compound WX016_4 (250.00 mg, 1.26 mmol), 1,5-cyclooctadiene (13.64 mg, 126.10 ?mol), and potassium hydroxide (84.90 mg, 1.51 mmol). The reaction mixture was heated to 60? C. The previously prepared catalyst was added thereto under nitrogen atmosphere, and the reaction mixture was stirred at 60? C. for 12 hours. After the reaction was complete, the reaction mixture was poured into water (10 mL) to quench the reaction, and extracted with ethyl acetate (10 mL?3). The organic phases were combined, washed with saturated brine (30 mL), dried over anhydrous sodium sulfate, filtered, and the filtrate was concentrated under reduced pressure to remove the solvent. The residue was purified by column chromatography (eluent: 19/1 to 7/3, v/v) to obtain compound WX016_6.

Step 5: Synthesis of Compound WX016_8

[0185] Compound WX001_5 (204.59 mg, 767.27 ?mol) was dissolved in anhydrous dichloromethane (4 mL) at room temperature under nitrogen atmosphere. The reaction mixture was cooled to 0? C., and oxalyl chloride (243.47 mg, 1.92 mmol, 167.91 ?L) was added dropwise thereto. A drop of N,N-dimethylformamide was then added thereto, and the reaction mixture was stirred at 20? C. for 1 hour. After the reaction was complete, the reaction mixture was directly concentrated under reduced pressure to remove the solvent. The residue was dissolved in anhydrous dichloromethane (5 mL). To a solution of compound WX016_6 (250.00 mg, 767.27 ?mol) and N,N-diisopropylethylamine (247.91 mg, 1.92 mmol, 334.10 ?L) in dichloromethane (2 mL) was added dropwise the prepared acyl chloride at 0? C. The reaction mixture was then stirred at 20? C. for 12 hours. After the reaction was complete, the reaction mixture was poured into water (10 mL) to quench the reaction. The phases were separated, and the organic phase was collected. The aqueous phase was extracted with dichloromethane (10 mL). The organic phases were then combined, washed with saturated brine (20 mL), dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated under reduced pressure to remove the solvent. The resulting residue was purified by column chromatography (eluent, petroleum ether/ethyl acetate=19/1 to 7/3, v/v) to obtain compound WX016_8.

Step 6: Synthesis of Compound WX016_10

[0186] Compound WX016_10 (300.00 mg, 522.23 ?mol) was dissolved in ethyl acetate (1 mL) at room temperature under nitrogen atmosphere. A solution of hydrochloric acid in ethyl acetate (4 M, 5 mL) was added thereto, and the reaction mixture was stirred at 20? C. for 5 hours. After the reaction was complete, the reaction mixture was directly concentrated under reduced pressure. The crude product was purified by preparative HPLC (mobile phase: acetonitrile/water; acidic system: 0.04% HCl) to obtain target compound WX016_10.

Step 7: Synthesis of Compounds WX016 and WX017

[0187] Compound WX016_10 was initially purified by chiral column chromatography (column type: Phenomenex-Cellulose-2 (250 mm?30 mm, 10 ?m); mobile phase: A (CO.sub.2) and B (ethanol containing 0.1% ammonia); gradient: B %=30% to 30%, 5 minutes) to obtain compounds WX016 and WX017, respectively.

[0188] SFC analysis method: column type: Lux Cellulose-2, 50?4.6 mm I.D., 3 ?m; mobile phase: A: CO.sub.2, B: [isopropanol (containing 0.1% IPAm)], gradient: B %: 5% to 50%, 5 minutes.

[0189] WX016 (retention time: 1.239 min): MS-ESI m/z: 517.9[M+H].sup.+. ee value: 99.66%. .sup.1H NMR (400 MHz, DMSO_d.sub.6) ?: 11.93 (s, 1H), 9.81 (s, 1H), 7.49-7.43 (m, 4H), 7.40 (d, J=1.9 Hz, 1H), 7.33 (d, J=8.3 Hz, 1H), 7.06 (dd, J=1.9, 8.3 Hz, 1H), 4.11 (d, J=10.6 Hz, 1H), 3.94-3.84 (m, 1H), 3.63-3.47 (m, 2H), 3.42-3.37 (m, 1H), 3.06-2.99 (m, 1H), 2.65-2.59 (m, 2H), 1.90-1.78 (m, 1H), 1.75-1.62 (m, 1H), 1.60-1.48 (m, 1H), 1.45-1.32 (m, 1H), 0.80 (d, J=7.1 Hz, 3H).

[0190] WX017 (retention time: 1.542 min): MS-ESI m/z: 517.9[M+H].sup.+. ee value: 99.70%. .sup.1H NMR (400 MHz, DMSO_d.sub.6) ?: 11.93 (s, 1H), 9.83 (s, 1H), 7.49-7.43 (m, 4H), 7.41 (d, J=1.8 Hz, 1H), 7.36 (d, J=8.3 Hz, 1H), 7.08 (dd, J=1.9, 8.4 Hz, 1H), 4.12 (d, J=10.5 Hz, 1H), 3.86-3.77 (m, 1H), 3.75-3.67 (m, 1H), 3.66-3.57 (m, 1H), 3.44-3.38 (m, 1H), 3.00-2.91 (m, 1H), 2.83-2.76 (m, 1H), 2.45-2.40 (m, 1H), 1.78-1.66 (m, 2H), 1.60-1.50 (m, 1H), 1.41-1.29 (m, 1H), 0.80 (d, J=7.1 Hz, 3H).

Step 8: Synthesis of Compound WX016_7

[0191] Chloro(1,5-cyclooctadiene)rhodium(I) dimer (15.54 mg, 31.52 ?mol) and 2,2-bis(diphenylphosphino)-1,1-binaphthalene (4.51 mg, 7.25 ?mol) were dissolved in tetrahydrofuran (3 mL) at room temperature under nitrogen atmosphere. The reaction mixture was stirred at 20? C. for 15 minutes. 3-Amino-4-chlorophenylboronic acid pinacol ester (319.70 mg, 1.26 mmol) was dissolved in a mixed solvent of isopropanol (1.5 mL) and tetrahydrofuran (3 mL) at room temperature under nitrogen atmosphere. To the reaction mixture were added compound WX016_5 (250.00 mg, 1.26 mmol), 1,5-cyclooctadiene (13.64 mg, 126.10 ?mol), and potassium hydroxide (84.90 mg, 1.51 mmol). The reaction mixture was heated to 60? C. The previously prepared catalyst was added thereto under nitrogen atmosphere, and the reaction mixture was stirred at 60? C. for 12 hours. After the reaction was complete, the reaction mixture was poured into water (10 mL) to quench the reaction, and extracted with ethyl acetate (10 mL?3). The organic phases were combined, washed with saturated brine (30 mL), dried over anhydrous sodium sulfate, filtered, and the filtrate was concentrated under reduced pressure to remove the solvent. The crude product was purified by column chromatography (eluent: 19/1 to 7/3, v/v) to obtain compound WX016_7.

Step 9: Synthesis of Compound WX016_9

[0192] Compound WX001_5 (109.66 mg, 411.26 ?mol) was dissolved in anhydrous dichloromethane (2 mL) at room temperature under nitrogen atmosphere, and the reaction mixture was cooled to 0? C. To the reaction mixture was added dropwise oxalyl chloride (156.60 mg, 1.23 mmol, 108.00 ?L), followed by the addition of a drop of N,N-dimethylformamide. The reaction system was stirred at 0? C. for 1 hour. After the reaction was complete, the reaction mixture was directly concentrated under reduced pressure to remove the solvent. The residue was dissolved in anhydrous dichloromethane (2 mL). Compound WX016_7 (134.00 mg, 411.26 ?mol) and N,N-diisopropylethylamine (132.88 mg, 1.03 mmol, 179.08 ?L) were sequentially added thereto at 0? C. The reaction mixture was then stirred at 25? C. for 12 hours. After the reaction was complete, the reaction mixture was directly concentrated under reduced pressure to remove the solvent. The resulting residue was purified by column chromatography (eluent, petroleum ether/ethyl acetate=19/1 to 7/3, v/v) to obtain target compound WX016_9.

Step 10: Synthesis of Compounds WX018 and WX019

[0193] Compound WX016_9 (174.00 mg, 302.89 ?mol) was dissolved in ethyl acetate (1 mL) at room temperature under nitrogen atmosphere. A solution of hydrochloric acid in ethyl acetate (4 M, 5 mL) was added thereto, and the reaction mixture was stirred at 25? C. for 1.5 hours. After the reaction was complete, the reaction mixture was directly concentrated under reduced pressure to remove the solvent. The resulting residue was purified by preparative HPLC (mobile phase: acetonitrile/water; acidic system: 0.04% HCl) to obtain target compounds WX018 and WX019.

[0194] SFC analysis method: column type: Chiralpak IC-3, 50?4.6 mm I.D., 3 ?m; mobile phase: A: CO.sub.2, B: [isopropanol (containing 0.1% IPAm)], gradient: B %: 5% to 50%, 3 minutes.

[0195] WX018 (retention time: 1.286 min): MS-ESI m/z: 517.9 [M+H].sup.+. ee value: 100%. .sup.1H NMR (400 MHz, DMSO_d.sub.6) ?: 12.03 (s, 1H), 9.81 (s, 1H), 7.49-7.43 (m, 4H), 7.43-7.41 (m, 1H), 7.33 (d, J=8.3 Hz, 1H), 7.06 (dd, J=2.0, 8.4 Hz, 1H), 4.12 (d, J=10.6 Hz, 1H), 3.94-3.86 (m, 1H), 3.62-3.47 (m, 2H), 3.41-3.34 (m, 1H), 3.07-2.98 (m, 1H), 2.63-2.60 (m, 1H), 2.57-2.53 (m, 1H), 1.90-1.78 (m, 1H), 1.75-1.62 (m, 1H), 1.60-1.49 (m, 1H), 1.45-1.34 (m, 1H), 0.80 (d, J=7.1 Hz, 3H).

[0196] WX019 (retention time: 1.226 min): MS-ESI m/z: 517.9 [M+H].sup.+. ee value: 92.60%. .sup.1H NMR (400 MHz, DMSO_d.sub.6) ?: 11.94 (s, 1H), 9.83 (s, 1H), 7.51-7.43 (m, 4H), 7.42-7.39 (m, 1H), 7.38-7.34 (m, 1H), 7.08 (dd, J=2.0, 8.4 Hz, 1H), 4.12 (d, J=10.6 Hz, 1H), 3.85-3.77 (m, 1H), 3.75-3.67 (m, 1H), 3.65-3.58 (m, 1H), 3.41-3.35 (m, 1H), 3.00-2.92 (m, 1H), 2.80 (dd, J=4.6, 15.9 Hz, 1H), 2.46-2.40 (m, 1H), 1.79-1.66 (m, 2H), 1.56 (dd, J=6.0, 13.5 Hz, 1H), 1.41-1.30 (m, 1H), 0.80 (d, J=7.1 Hz, 3H).

Bioassay:

Test Example 1: In Vitro Activity Assay

I. cGMP Expression Test Based on InCap Cells

1. Experimental Steps

1) Solution Preparation

10% BSA (Bovine Serum Albumin)

[0197] 10 g of BSA was dissolved in 100 mL of double-distilled water (ddH.sub.2O) to obtain 10% BSA.

10 mM ODQ Stock Solution

[0198] 1 mg of ODQ powder was weighed and dissolved in 534 ?L of DMSO to prepare 10 mM ODQ solution. The solution was aliquoted and stored in a ?20? C. refrigerator.

Washing Buffer (50 mL)

[0199]

TABLE-US-00001 Volume Final concentration 49 mL of Earls balanced 1x salt solution (EBSS) 500 ?L of 1M hydroxyethyl piperazine 10 mM ethanesulfonic acid (HEPES) 250 ?L of 10% BSA stabilizer 0.05% 250 ?L of 1M MgCl.sub.2 5 mM

Assay Buffer (50 mL)

[0200]

TABLE-US-00002 Volume Final concentration 48.95 mL of Earls balanced 1x salt solution (EBSS) 500 ?L of 1M hydroxyethyl piperazine 10 mM ethanesulfonic acid (HEPES) 250 ?L 10% BSA 0.05% 50 ?L of 500 mmol/L 0.5 mM isobutylmethylxanthine (IBMX) 250 ?L of 1M MgCl.sub.2 5 mM

Detection Buffer

[0201] a) 50 ?L of cGMP-D2 (D2-labeled cyclic guanosine monophosphate) was added to 1 mL of lysis buffer and mixed thoroughly.

[0202] b) 50 ?L of anti-cGMP cryptate (Eu.sup.3+ cryptate-labeled anti-cyclic guanosine monophosphate antibody) was added to 1 mL of lysis buffer and mixed thoroughly.

2) Compound Dilution

[0203] (1) The compound was diluted to a concentration of 10 mM using DMSO.

[0204] (2) A serial dilution of the compound was performed, resulting in 10 different concentration levels of each compound. 100 nL of each diluted compound was added to a 96-well plate.

3) Preparation of LNCap Cells

[0205] (1) LNCap culture medium: RPMI1640 supplemented with 10% fetal bovine serum (FBS) and 1% double antibody

[0206] (2) The phosphate-buffered saline (PBS), trypsin, and culture medium for use in cell passage were preheated in a 37? C. water bath.

[0207] (3) Cells were removed from a 37? C., 5% Co.sub.2 incubator, and the old culture medium was removed from the culture flask using a pipette.

[0208] (4) 5 mL of PBS was pipetted into the flask for rinsing the cells, and then the liquid was discarded.

[0209] (5) 3 mL of trypsin was pipetted into the flask. After shaking, the liquid was discarded, and the flask was returned to the incubator.

[0210] (6) Approximately 2 minutes later, the flask was retrieved, and cell detachment was observed. 9 mL of culture medium was pipetted into the culture flask and mixed by repetitive pipetting. The cell suspension was then transferred to a 50 mL centrifuge tube.

[0211] (7) 0.7 mL of cell suspension was pipetted into a counting chamber, and cell counting was performed using the ViCell XR. The remaining cells were centrifuged at 1000 rpm for 5 minutes, and the supernatant was discarded.

[0212] (8) 10 mL of washing buffer was added to wash the cells, followed by another centrifugation at 1000 rpm for 5 minutes, after which the supernatant was discarded.

[0213] (9) Assay buffer was added, and the cell concentration was adjusted to 3?10.sup.6/mL.

4) Preparation and Addition of ODQ Solution

[0214] (1) 10 mM ODQ stock solution was diluted at a ratio of 1:1000 and added to the cell solution.

[0215] (2) After thorough mixing, the solution was added to a microplate at 10 ?L per well.

5) Preparation of cGMP Standard Curve

[0216] (1) 1 mM cGMP stock solution was diluted to 10 ?M using assay buffer. 11 concentration gradients were created through 4-fold serial dilutions.

[0217] (2) The diluted cGMP was added to the microplate at 10 ?L per well.

6) Addition of Assay Reagents and Plate Reading

[0218] (1) cGMP-D2 was transferred to a 384-well plate at 5 ?L per well. Anti-cGMP cryptate was transferred to a 96-well plate at 5 ?L per well. Centrifugation was performed at 1500 rpm for 1 minute.

[0219] (2) Incubation was carried out at room temperature for 1 hour.

[0220] (3) The plates were read using envision at 665/615 wavelengths.

7) Data Analysis

[0221] (1) cGMP standard curve: A standard curve was generated using GraphPad Prism, based on the ratio of cGMP concentration to the 665/615 wavelength values.

[0222] (2) Conversion of HTRF (homogeneous time-resolved fluorescence) ratio (665/615) to cGMP concentration: In GraphPad Prism, the HTRF ratio (665/615) was copied to the ratio column of the cGMP standard curve. The analysis Log inhibitor vs response-variable slope was run, and the interpolate option was selected to convert the HTRF ratio (665/615) into cGMP concentration.

[0223] (3) Compound activation curve: A curve was generated using the Log agonist vs response-variable slope analysis method in GraphPad Prism, based on the converted cGMP concentration and compound concentration.

TABLE-US-00003 TABLE 1 MEC values of the compounds of the present disclosure for sGC stimulatory activity Compound MEC (nM) EC50 (nM) WX001 4.20 6.62 WX002 2.20 1.76 WX003 NA 27.72 WX004 0.61 3.74 WX005 0.27 2.06 WX006 7.92 33.60 WX007 0.65 3.28 WX008 0.95 4.14 WX009 33.06 28.96 WX010 1.76 2.16 WX011 1.39 1.75 WX012 25.92 66.08 WX013 1.22 3.50 WX014 0.42 1.65 WX015 0.82 16.71 WX016 27.85 145.0 WX019 9.83 81.24 MEC: Minimum effective concentration required to stimulate cGMP production in LNCap cells (greater than three times the baseline value). NA indicates not measured.

[0224] Experimental conclusion: The compounds of the present disclosure are effective in stimulating sGC, thereby increasing cGMP levels.

Test Example 2: In Vitro Hepatocyte Metabolic Stability Study

1. Experimental Objective: The Aim of the Study was to Assess the Stability of the Compounds in Hepatocytes of Different Species.

2. Experimental Steps:

[0225] A variety of 96-well precipitation plates were prepared and labeled as T0, T15, T30, T60, T90, T0-MC, T90-MC, and blank matrix, respectively. Revival medium and incubation medium were taken in advance and pre-warmed in a 37? C. water bath. Hepatocytes of different species, stored in a liquid nitrogen tank, were taken and immediately immersed in a 37? C. water bath for approximately 90 seconds. Once partially thawed, cells were transferred to centrifuge tubes containing 40 mL of revival medium, and gently inverted to resuspend the cells in the revival medium. At room temperature, cells were centrifuged at 100?g for 5 minutes. The supernatant was discarded, and the hepatocytes were resuspended in an appropriate volume of incubation medium. Viability was calculated using trypan blue staining. 198 ?L of hepatocyte suspension (0.51?10.sup.6 cells/mL) was added to pre-warmed incubation plates. For the control group, 198 ?L of hepatocyte-free incubation medium was added to T0-MC and T120-MC plates. All plates were pre-incubated for 10 minutes in a 37? C. incubator. 2 ?L of test and control compound working solutions were then added, mixed thoroughly, and the incubation plates were immediately placed in a shaker within the incubator. The timer was initiated to start the reaction. Two duplicate samples were prepared at each time point for each compound. Incubation conditions were set at 37? C., saturated humidity, and 5% CO.sub.2. In the test system, the test compound had a final concentration of 1 ?M, the control compound had a final concentration of 3 ?M, the hepatocyte had a final concentration of 0.5?10.sup.6 cells/mL, and the total organic solvent had a final concentration of 0.96%, of which DMSO had a final concentration of 0.1%. At the end of the incubation at the corresponding time point, the incubation plate was removed, and 25 ?L of the compound and control compound-cell mixture was transferred to a sample plate containing 125 ?L of stop solution (acetonitrile solution containing 200 ng/mL of tolbutamide and Labetalol). For the blank sample plate, 25 ?L of hepatocyte-free incubation medium was directly added. All sample plates were sealed and shaken on the shaker at 600 rpm for 10 minutes, and then centrifuged at 3220?g for 20 minutes. The supernatant of test and control samples was diluted with ultrapure water at a ratio of 1:3. All samples were mixed well and analyzed using LC/MS/MS methods.

[0226] The experimental results are shown in Table 2.

TABLE-US-00004 TABLE 2 Stability of the compounds of the present disclosure in hepatocytes of different species Species Test item WX005 WX015 Mouse T.sub.1/2-min 27.0 85.2 CL.sub.int (liver) 610.4 193.3 (mL/min/kg) Rat T.sub.1/2-min 41.9 78.2 CL.sub.int (liver) 154.7 82.9 (mL/min/kg) Human T.sub.1/2-min 77.6 154.7 CL.sub.int (liver) 49.7 24.9 (mL/min/kg) T.sub.1/2: half-life; CL.sub.int (liver): hepatic clearance

[0227] Experimental conclusion: The compounds of the present disclosure exhibit good stability in human-derived hepatocytes, with moderate hepatic clearance and half-life.

Test Example 3: In Vivo Pharmacokinetic Property Study

[0228] Experimental objective: The aim of this study was to determine the pharmacokinetic parameters of the compound in male Sprague Dawley rats.

Experimental Materials:

[0229] Sprague Dawley rats (male, 200 to 300 g, 7 to 9 weeks old, procured from Shanghai SLAC)

Experimental Methods:

[0230] For this project, four male Sprague Dawley rats were used. Two rats in one group were administered the compound intravenously at a dosage of 2 mg/kg and a concentration of 0.4 mg/mL. Two rats in another group were administered the compound orally at a dosage of 10 mg/kg and a concentration of 1 mg/mL. Plasma samples were collected 0.083 (intravenous group only), 0.25, 0.5, 1, 2, 4, 6, 8, and 24 hours after administration. The collected samples were then analyzed using LC-MS/MS and data were collected. The collected analytical data were processed using Phoenix WinNonlin 6.3 software to calculate relevant pharmacokinetic parameters.

[0231] The experimental results are shown in Table 3.

TABLE-US-00005 TABLE 3 Results of in vivo pharmacokinetic experiments Test sample WX005 WX015 iv Cl 5.47 6.7 (2 mg/kg) (mL/min/kg) V.sub.dss (L/kg) 1.27 0.67 T.sub.1/2 (h) 3.83 2.38 AUC 11228 9147 (h*nmol/L) po T.sub.1/2 (h) 6.92 2.72 (10 mg/kg) AUC 17423 36252 (h*nmol/L) % F 42.1% 79.4%

[0232] Conclusion: The compounds of the present disclosure exhibit favorable clearance, half-life, and oral bioavailability by gavage.