COSMECEUTICAL COMPOSITION

Abstract

The present invention relates to a cosmeceutical composition, comprising, relative to the total weight of said composition: collagen, in an amount between 620 wt and 760 wt ; saline solution, in an amount between 225 wt and 350 wt ; hyaluronic acid or a pharmaceutically acceptable salt thereof, in an amount between 1 wt and 3 wt ; and at least one natural anti-inflammatory and anti-oxidant agent.

Claims

1. A cosmeceutical composition, comprising, relative to the total weight of said composition: collagen, in an amount between 620 wt and 760 wt ; saline solution, in an amount between 225 wt and 350 wt ; hyaluronic acid or a pharmaceutically acceptable salt thereof, in an amount between 1 wt and 3 wt ; and at least one natural anti-inflammatory and anti-oxidant agent.

2. The composition according to claim 1, wherein the at least one natural anti-inflammatory and anti-oxidant agent is selected from the group consisting of: turmeric (Curcuma longa) rhizome extract, Ginkgo Biloba leaf extract, and vitamin C.

3. The composition according to claim 2, wherein the turmeric rhizome extract is present in an amount between 2.1 wt and 3.9 wt , preferably 2.3 wt and 3.7 wt , more preferably between 2.4 wt and 3.5 wt relative to the total weight of the composition.

4. The composition according to claim 2, wherein said Ginkgo Biloba leaf extract is present in an amount between 0.0015 wt and 0.0080 wt , preferably between 0.0020 wt and 0.0065 wt , relative to the total weight of the composition.

5. The composition according to claim 2, wherein the ratio by weight between said turmeric rhizome extract and said Ginkgo Biloba leaf extract is between 100:1 and 2000:1, preferably between 200:1 and 1700:1, even more preferably between 300:1 and 1500:1.

6. The composition according to claim 2, wherein said vitamin C is present in an amount between 5 wt and 7 wt , preferably in an amount between 5.3 wt and 6.8 wt relative to the total weight of the composition.

7. The composition according to claim 1, further comprising vitamin D, preferably vitamin D3.

8. The composition according to claim 7, wherein said vitamin D is in an amount between 0.0010 wt and 0.0080 wt , preferably in an amount between 0.0015 wt and 0.0075 wt , even more preferably between 0.0020 wt and 0.0070 wt relative to the total weight of the composition.

9. The composition according to claim 1, wherein said hyaluronic acid or a pharmaceutically acceptable salt thereof is in an amount between 1 wt and 2.5 wt relative to the total weight of the composition.

10. The composition according to claim 1, wherein said collagen is hydrolyzed collagen, preferably type I hydrolyzed collagen.

11. The composition according to claim 1, wherein said collagen, preferably hydrolyzed collagen is in an amount between 650 wt and 750 wt relative to the total weight of the composition.

12. The composition according to claim 1, comprising relative to the total weight of said composition: collagen, preferably hydrolyzed collagen, in an amount between 650 wt and 750 wt ; saline solution in an amount between 240 wt and 330 wt; hyaluronic acid or a pharmaceutically acceptable salt thereof in an amount between 1 wt and 2.5 wt ; turmeric (Curcuma longa) rhizome extract in an amount between 2.4 wt and 3.5 wt ; Ginkgo biloba leaf extract in an amount between 0.0020 wt and 0.0065 wt . vitamin C in an amount between 5.3 wt and 6.8 wt ; and vitamin D, preferably vitamin D3, in an amount of 0.0020 wt and 0.0070 wt .

13. A method of manufacturing the cosmeceutical composition according to claim 1, comprising: a) a step of mixing together: collagen, in an amount between 620 wt and 760 wt relative to the total weight of the composition; hyaluronic acid or a pharmaceutically acceptable salt thereof, in an amount between 1 wt and 3 wt relative to the total weight of the composition; and at least one natural anti-inflammatory and anti-oxidant agent, the step of mixing causing the production of a powder mixture; b) a step of adding a saline solution, in an amount between 225 wt and 350 wt relative to the total weight of the composition, to said powder mixture obtained according to step a); c) a step of uniformly mixing the saline solution with the powder mixture, obtaining the cosmeceutical composition.

14. The method according to claim 13, wherein the anti-inflammatory and anti-oxidant agent according to step a) is selected from the group consisting of: turmeric (Curcuma longa) rhizome extract, Ginkgo Biloba leaf extract and vitamin C, wherein turmeric rhizome extract is preferably added in an amount between 2.1 wt and 3.9 wt , more preferably 2.3 wt and 3.7 wt , even more preferably between 2.4 wt and 3.5 wt relative to the total weight of the composition, and/or wherein Ginkgo Biloba leaf extract is preferably added in an amount between 0.0015 wt and 0.0080 wt , more preferably between 0.0020 wt and 0.0065 wt , relative to the total weight of the composition, and/or vitamin C is preferably added in an amount between 5 wt and 7 wt , more preferably in an amount between 5.3 wt and 6.8 wt relative to the total weight of the composition.

15. The method according to claim 13, wherein the step a) of mixing further includes mixing vitamin D, preferably vitamin D3, in an amount between 0.0010 wt and 0.0080 wt , preferably in an amount between 0.0015 wt and 0.0075 wt , even more preferably between 0.0020 wt and 0.0070 wt relative to the total weight of the composition.

16. The method according to claim 13, comprising a step of hydrolyzing said collagen.

Description

DETAILED DESCRIPTION

[0065] The present disclosure relates to a cosmeceutical composition, comprising, in its simplest form: collagen, hyaluronic acid or a pharmaceutically acceptable salt thereof, saline solution and at least natural anti-inflammatory and anti-oxidant agent.

[0066] Object of the present disclosure is a cosmeceutical composition comprising relative to the total weight of said composition: [0067] collagen, in an amount between 620 wt and 760 wt ; [0068] saline solution, in an amount between 225 wt and 350 wt ; [0069] hyaluronic acid or a pharmaceutically acceptable salt thereof, in an amount between 1 wt and 3 wt ; and [0070] at least one natural anti-inflammatory and anti-oxidant agent.

[0071] The composition according to the disclosure stimulates the production of elastin, collagen and hyaluronic acid, restores fibroblast elasticity and protects the skin from free radicals (skin aging).

[0072] According to a preferred embodiment, the present composition comprises heterologous collagen. Collagen may be extracted from fish, bovine and/or chicken, preferably from costal cartilages of chickens.

[0073] According to a particularly preferred embodiment, the present composition comprises hydrolyzed collagen.

[0074] Hydrolyzed collagen is obtained by making type I native collagen (undenatured collagen) undergo a process of thermal, acid, alkaline or enzymatic hydrolysis; such process allows to reduce the collagen in protein fragments of a lower molecular weight.

[0075] By means of the hydrolysis, the long chains of the collagen are reduced in smaller particles. These particles, or fragments, are defined biomimetic since they simulate the degradation action of polymerized collagen, massively stimulate fibroblast and pro-collagen deposit and, consequently, stimulate type Ill endogenous collagen, substantially eliminating the risk of allergies.

[0076] In this way, the behavior of the peptide chains which are part of the collagen is enhanced, and a better cutaneous absorption of said peptide chains is thereby achieved.

[0077] At the skin level, hydrolyzed collagen helps to maintain and improve the firmness, tone, and skin turgor while minimizing and improving the presence of wrinkles.

[0078] According to a preferred embodiment, said collagen is type I collagen. Applicant notes that anyway said collagen may be, alternatively or in combination, type II and/or type III collagen.

[0079] Type I collagen is the collagen that is present in the skin, tendons and bone tissue, and is the collagen type that is most easily recognizable by human immune system.

[0080] Type I collagen contributes to keep and increase the compactness, tonicity, firmness of the skin, and thus reduces the presence of wrinkles.

[0081] Type II collagen may be of a native type and may be suitable to stimulate fibroblasts to create new tissues; thus native collagen may be capable of exerting a relevant biorestructuration activity.

[0082] Type Ill collagen is the second most abundant collagen of soft tissues and dominates early phases of wound healing and granulation tissue formation.

[0083] Applicant notes that skin is composed of about 80% of type I collagen, responsible of force and integrity of the skin; the remaining 20% is type Ill collagen, which is responsible for skin stretching.

[0084] The composition according to the disclosure comprises collagen, in particular hydrolyzed collagen, in an amount between 620 wt and 760 wt , preferably between 650 wt and 750 wt relative to the total weight of the composition.

[0085] Hyaluronic acid is an anionic, nonsulfated glycosaminoglycan widely distributed throughout human connective, epithelial, and neural tissues. Advantageously, hyaluronic acid gives the skin its special properties of strength and shape maintenance. Its lack results in a weakening of the skin by promoting the formation of wrinkles and blemishes.

[0086] According to a particularly preferred embodiment, the composition according to the disclosure comprises the sodium salt of hyaluronic acid, sodium hyaluronate.

[0087] Sodium hyaluronate is a hydrophilic molecule, with a standing out hygroscopic behavior. A molecule of sodium hyaluronate is capable of retaining up to ten thousands molecules of water. For such reason, it shows moistening properties, absorbs and retains moisture and determines a supply of water in the skin.

[0088] According to an embodiment, the composition comprises sodium hyaluronate powder.

[0089] Hyaluronic acid or a pharmaceutically acceptable salt thereof is present in an amount between 1 wt and 3 wt , preferably between 1 wt and 2.5 wt relative to the total weight of the composition.

[0090] The saline solution, according to a preferred embodiment, may be a 0.9% saline solution of NaCl with distilled water, of pharmaceutical grade. Saline solution is present in an amount between 225 wt and 350 wt , more preferably between 240 wt and 330 wt, relative to the total weight of the composition.

[0091] In an embodiment, the sum of hyaluronic acid or a pharmaceutically acceptable salt thereof, saline solution, collagen and anti-oxidant and anti-inflammatory agent reaches the 1000 wt.

[0092] The at least one natural anti-inflammatory and anti-oxidant agent which is present in the composition according to the disclosure is selected from the group consisting of turmeric (in particular the extract of the rhizome of the Curcuma longa variety), Ginkgo biloba leaf extract and vitamin C.

[0093] Turmeric, and in particular the extract of the rhizome of the Curcuma longa variety, has shown several beneficial properties for the skin. In particular, it has shown anti-wrinkles, anti-flaccidity, anti-inflammatory and/or anti-irritation properties. Moreover, turmeric shows hydrating properties.

[0094] The composition according to the disclosure comprises turmeric (Curcuma longa) rhizome extract in an amount between 2.1 wt and 3.9 wt , more preferably 2.3 wt and 3.7 wt , even more preferably between 2.4 wt and 3.5 wt , relative to the total weight of said composition.

[0095] Vitamin C, i.e. the L enantiomer of the ascorbic acid, has several anti-oxidant properties and acts against the skin photo-chromic ageing, effectively protecting the skin against the damages caused by free radicals caused by sun exposure and pollution. At the same time, vitamin C shows anti-wrinkles properties. The brightening effect, determines that dark spots on the skin become lighter, less visible.

[0096] When present, vitamin C is present in an amount between 5 wt and 7 wt , more preferably in an amount between 5.3 wt and 6.8 wt relative to the total weight of said composition.

[0097] In a preferred embodiment, the cosmeceutical composition comprises vitamin C powder.

[0098] Ginkgo Biloba leaf extract helps the elasticity of the skin, by stimulating cell renewing. Therefore, it acts against skin ageing.

[0099] Ginkgo Biloba leaf extract contains many different flavone glycosides and terpenoids.

[0100] In particular, Ginkgo Biloba leaf extract has anti-inflammatory and anti-oxidant properties and increases blood microcirculation and skin oxygenation. Ginkgo Biloba leaf extract shows effects against free radicals that produces beneficial effects to the cell metabolism, and prevents lipid peroxidation of the membranes.

[0101] Ginkgo Biloba leaf extract shows beneficial effects against the reactive forms of the oxygen (ROS), sustaining the normal function of fibroblasts.

[0102] Ginkgo Biloba leaf extract has been mainly selected for the anti-inflammatory and anti-oxidant properties, it may be selected as an additional ingredient due to the increase in microcirculation and skin oxygenation.

[0103] According to the disclosure, the composition comprises Ginkgo biloba leaf extract powder and/or turmeric rhizome extract powder.

[0104] In the composition according to the disclosure, Ginkgo Biloba leaf extract powder may be used as an alternative to the turmeric rhizome extract powder or as a further additive element, in an amount between 0.0015 wt and 0.0080 wt , preferably between 0.0020 wt and 0.0065 wt , relative to the total weight of the composition.

[0105] In particular, turmeric rhizome extract powder and Ginkgo Biloba leaf extract powder are combined with a ratio by weight between 100:1 and 2000:1, preferably between 200:1 and 1700:1, even more preferably between 300:1 and 1500:1. As it will be apparent by further reading the present description, example 1 of the composition is provided with turmeric rhizome extract and Ginkgo Biloba leaf extract in a ratio by weight of 1000:1.

[0106] Preferably, the anti-oxidant and anti-inflammatory agent is turmeric rhizome extract and/or vitamin C.

[0107] In addition to the aforementioned components, the Applicant has found that several other elements may be beneficially combined thereto. In particular, the composition according to the disclosure may further comprise vitamin D, and in particular D3.

[0108] Vitamin D has a fundamental role in the skin barrier and in the growth of skin cells, and sustains local immune system.

[0109] When present, vitamin D, preferably vitamin D3, is present in an amount between 0.0010 wt and 0.0080 wt ; preferably between 0.0015 wt and 0.0075 wt , more preferably between 0.0020 and 0.0070 wt relative to the total weight of said composition.

[0110] The composition herein disclosed is a liquid composition that may be injected subcutaneously by means of very small needles; the use of very small needles causes less discomfort in the patient during the treatment.

[0111] In a preferred embodiment, the composition comprises relative to the total weight of said composition: [0112] collagen, preferably hydrolyzed collagen, in an amount between 650 wt and 750 wt [0113] saline solution in an amount between 240 wt and 330 wt; [0114] hyaluronic acid or a pharmaceutically acceptable salt thereof in an amount between 1 wt and 2.5 wt ; [0115] turmeric (Curcuma longa) rhizome extract in an amount between 2.4 wt and 3.5 wt ; [0116] Ginkgo biloba leaf extract in an amount between 0.0020 wt and 0.0065 wt . [0117] vitamin C in an amount between 5.3 wt and 6.8 wt ; and [0118] vitamin D, preferably vitamin D3, in an amount of 0.0020 wt and 0.0070 wt .

[0119] In an embodiment, the composition comprises collagen, preferably hydrolyzed collagen, saline solution, hyaluronic acid or a pharmaceutically acceptable salt thereof, turmeric (Curcuma longa) rhizome extract, vitamin C, and vitamin D, preferably vitamin D3.

[0120] In another embodiment, the composition consist of hydrolyzed collagen, hyaluronic acid or a pharmaceutically acceptable salt thereof, saline solution, turmeric (Curcuma longa) rhizome extract, vitamin C, and vitamin D3.

[0121] In another embodiment, the composition consist of hydrolyzed collagen, hyaluronic acid or a pharmaceutically acceptable salt thereof, saline solution, turmeric (Curcuma longa) rhizome extract, Gingko Biloba leaf extract, vitamin C, and vitamin D3.

[0122] The composition according to the disclosure may comprise one or more cosmeceutical excipients, wherein said excipient refers to any compound that is acceptable in the sense of being compatible with the other ingredients of the composition.

[0123] The present disclosure further refers to a method of manufacturing a cosmeceutical composition, comprising relative to the total weight of said composition: [0124] a) a step of mixing together: [0125] collagen, in an amount between 620 wt and 760 wt relative to the total weight of the composition; [0126] hyaluronic acid or a pharmaceutically acceptable salt thereof, in an amount between 1 wt and 3 wt relative to the total weight of the composition; and [0127] at least one natural anti-inflammatory and anti-oxidant agent, the step of mixing causing the production of a powder mixture; [0128] b) a step of adding saline solution to said powder mixture, in an amount between 225 wt and 350 wt relative to the total weight of the composition; [0129] c) a step of uniformly mixing the saline solution with the powder mixture, obtaining the cosmeceutical composition.

[0130] The at least one natural anti-inflammatory and anti-oxidant agent is selected from the group consisting of turmeric (Curcuma longa) rhizome extract, vitamin C and Ginkgo Biloba leaf extract.

[0131] Preferably, turmeric rhizome extract is added in an amount between 2.1 wt and 3.9 wt , preferably between 2.3 wt and 3.7 wt , even more preferably between 2.4 wt and 3.5 wt , relative to the total weight of the composition, and/or Ginkgo Biloba leaf extract is added in an amount between 0.0015 wt and 0.0080 wt , preferably between 0.0020 wt and 0.0065 wt relative to the total weight of the composition, and/or vitamin C is added in an amount between 5 wt and 7 wt , preferably in an amount between 5.3 wt and 6.8 wt relative to the total weight of the composition.

[0132] The method may further include the step of mixing vitamin D, preferably vitamin D3, together with the components mixed in step a).

[0133] According to this embodiment, vitamin D, preferably vitamin D3, is mixed in an amount between 0.0010 wt and 0.0080 wt , preferably in an amount between 0.0015 wt and 0.0075 wt , even more preferably between 0.0020 wt and 0.0070 wt relative to the total weight of the composition.

[0134] The composition as above defined may be advantageously used for skin restructuration and revitalization, in particular for facial skin, neck, decollete, and hands restructuration and revitalization.

[0135] In particular, the composition above described may be used in a method for skin biorevitalization and biorestructuration comprising performing subcutaneous microinjections of the composition above described to a subject in need thereof.

[0136] Said subcutaneous microinjections may be carried out preferably at a distance of 0.5 to 5 cm one another, preferably at a distance of 1 to 3 cm, even more preferably at a distance of 1 cm one another.

[0137] In particular, before said subcutaneous microinjections, the subject in need thereof may be subjected to anesthesia, for example local anesthesia.

[0138] Said subcutaneous microinjections of the composition according to the disclosure may be preferably carried out on facial skin, neck, decollete, and hands.

[0139] The treatment may be carried out 4 or 5 times, at intervals of about 1 month, following the first 4 or 5 treatments, a sustainment treatment may be further carried out with the same procedure at an interval of about 6 months. Should the patient so request, a pre-treatment with local anesthetic may be carried out. Applicant has observed that the composition herewith disclosed is compatible with the traditional anesthetic skin creams without side effects or adverse interactions.

EXAMPLES

[0140] Hereinafter, some embodiments of the composition according to the disclosure are provided.

[0141] The hydrolyzed collagen used for the compositions below is the product commercialized under the trademark name Glow25?.

Example 1

[0142] A first and preferred example of the composition comprises: [0143] 10000 mg of hydrolyzed collagen Glow25? [0144] 25 mg of hyaluronic acid [0145] 40 mg of turmeric (Curcuma longa) rhizome extract [0146] 80 mg of vitamin C [0147] 50 ?g of vitamin D3 [0148] 40 ?g of Ginkgo Biloba leaf extract [0149] 3854.91 mg of saline solution

[0150] In particular, the saline solution is a 0.9% saline solution of NaCl with distilled water.

[0151] The above amounts allow obtaining a 14 g injectable composition.

[0152] If the above amounts are provided in terms of by weight, it is clear that the first and preferred example of the composition comprises: [0153] 714.29 of hydrolyzed collagen Glow25? [0154] 1.786 of hyaluronic acid [0155] 2.857 of turmeric (Curcuma longa) rhizome extract [0156] 5.714 of vitamin C [0157] 0.0035714 of vitamin D3 [0158] 0.0028572 of Ginkgo Biloba leaf extract [0159] 275.35 of saline solution

[0160] In the first example, hyaluronic acid is in the form of sodium hyaluronate powder.

Example 2

[0161] A second example of the composition comprises: [0162] 10000 mg of hydrolyzed collagen Glow25? [0163] 25 mg of hyaluronic acid [0164] 40 mg of turmeric (Curcuma longa) rhizome extract [0165] 80.05 mg of vitamin C [0166] 40 ?g of vitamin D3 [0167] 3854.91 mg of saline solution

[0168] In particular, the saline solution is a 0.9% saline solution of NaCl with distilled water.

[0169] The above amounts allow obtaining a 14 g injectable composition.

[0170] If the above amounts are provided in terms of by weight, it is clear that the first and preferred example of the composition comprises: [0171] 714.29 of hydrolyzed collagen Glow25? [0172] 1.786 of hyaluronic acid [0173] 2.857 of turmeric (Curcuma longa) rhizome extract [0174] 5.718 of vitamin C [0175] 0.0028572 of vitamin D3 [0176] 275.35 of saline solution

Example 3

[0177] A third example of the composition comprises: [0178] 10000 mg of hydrolyzed collagen Glow25? [0179] 25 mg of hyaluronic acid [0180] 40 mg of turmeric (Curcuma longa) rhizome extract [0181] 80 mg of vitamin C [0182] 90 ?g of vitamin D3 [0183] 3854.91 mg of saline solution

[0184] In particular, the saline solution is a 0.9% saline solution of NaCl with distilled water.

[0185] The above amounts allow obtaining a 14 g injectable composition.

[0186] If the above amounts are provided in terms of by weight, it is clear that the first and preferred example of the composition comprises: [0187] 714.29 of hydrolyzed collagen Glow25? [0188] 1.786 of hyaluronic acid [0189] 2.857 of turmeric (Curcuma longa) rhizome extract [0190] 5.714 of vitamin C [0191] 0.0064286 of vitamin D3 [0192] 275.35 of saline solution

Example 4

[0193] A fourth example of the composition comprises: [0194] 10000 mg of hydrolyzed collagen Glow25? [0195] 25 mg of hyaluronic acid [0196] 40 mg of turmeric (Curcuma longa) rhizome extract [0197] 80 mg of vitamin C [0198] 90 ?g of Ginkgo Biloba leaf extract [0199] 3854.91 mg of saline solution

[0200] In particular, the saline solution is a 0.9% saline solution of NaCl with distilled water.

[0201] The above amounts allow obtaining a 14 g injectable composition.

[0202] If the above amounts are provided in terms of by weight, it is clear that the first and preferred example of the composition comprises: [0203] 714.29 of hydrolyzed collagen Glow25? [0204] 1.786 of hyaluronic acid [0205] 2.857 of turmeric (Curcuma longa) rhizome extract [0206] 5.714 of vitamin C [0207] 0.0064286 of Ginkgo Biloba leaf extract [0208] 275.35 of saline solution

[0209] In the fourth example, hyaluronic acid is in the form of sodium hyaluronate powder.

Example 5

[0210] A fifth example of the composition comprises: [0211] 9992 mg of hydrolyzed collagen Glow25? [0212] 20 mg of hyaluronic acid [0213] 85 mg of vitamin C [0214] 50 ?g of vitamin D3 [0215] 40 ?g of Ginkgo Biloba leaf extract [0216] 3902.91 mg of saline solution

[0217] In particular, the saline solution is a 0.9% saline solution of NaCl with distilled water.

[0218] The above amounts allow obtaining a 14 g injectable composition.

[0219] If the above amounts are provided in terms of by weight, it is clear that the first and preferred example of the composition comprises: [0220] 713.71 of hydrolyzed collagen Glow25? [0221] 1.429 of hyaluronic acid [0222] 6.071 of vitamin C [0223] 0.0035714 of vitamin D3 [0224] 0.0028572 of Ginkgo Biloba leaf extract [0225] 278.77 of saline solution

[0226] In the fifth example, hyaluronic acid is in the form of sodium hyaluronate powder.

Example 6

[0227] A sixth example of the composition comprises: [0228] 8000 mg of hydrolyzed collagen Glow25? [0229] 25 mg of hyaluronic acid [0230] 40 mg of turmeric (Curcuma longa) rhizome extract [0231] 80 mg of vitamin C [0232] 50 ?g of vitamin D3 [0233] 40 ?g of Ginkgo Biloba leaf extract [0234] 3854.91 mg of saline solution

[0235] In particular, the saline solution is a 0.9% saline solution of NaCl with distilled water.

[0236] The above amounts allow obtaining a 12 g injectable composition.

[0237] If the above amounts are provided in terms of by weight, it is clear that the first and preferred example of the composition comprises: [0238] 667 of hydrolyzed collagen Glow25? [0239] 2.083 of hyaluronic acid [0240] 3.333 of turmeric (Curcuma longa) rhizome extract [0241] 6.666 of vitamin C [0242] 0.0041666 of vitamin D3 [0243] 0.0033333 of Ginkgo Biloba leaf extract [0244] 321.24 of saline solution

[0245] In the sixth example, hyaluronic acid is in the form of sodium hyaluronate powder.

Example 7

[0246] A seventh example of the composition comprises: [0247] 11500 mg of hydrolyzed collagen Glow25? [0248] 25 mg of hyaluronic acid [0249] 40 mg of turmeric (Curcuma longa) rhizome extract [0250] 80 mg of vitamin C [0251] 40 ?g of vitamin D3 [0252] 50 ?g of Ginkgo Biloba leaf extract [0253] 3900 mg of saline solution

[0254] In particular, the saline solution is a 0.9% saline solution of NaCl with distilled water.

[0255] The above amounts allow obtaining a 15.5451 g injectable composition.

[0256] If the above amounts are provided in terms of by weight, it is clear that the first and preferred example of the composition comprises: [0257] 739.8 of hydrolyzed collagen Glow25? [0258] 1.608 of hyaluronic acid [0259] 2.573 of turmeric (Curcuma longa) rhizome extract [0260] 5.146 of vitamin C [0261] 0.002573 of vitamin D3 [0262] 0.003216 of Ginkgo Biloba leaf extract [0263] 250.88 of saline solution

[0264] In the seventh example, hyaluronic acid is in the form of sodium hyaluronate powder.

Example 8

[0265] A eighth example of the composition comprises: [0266] 10000 mg of hydrolyzed collagen Glow25? [0267] 25 mg of hyaluronic acid [0268] 40 mg of turmeric (Curcuma longa) rhizome extract [0269] 50 ?g of vitamin D3 [0270] 30 ?g of Ginkgo Biloba leaf extract [0271] 3934 mg of saline solution

[0272] In particular, the saline solution is a 0.9% saline solution of NaCl with distilled water.

[0273] The above amounts allow obtaining a 14 g injectable composition.

[0274] If the above amounts are provided in terms of by weight, it is clear that the first and preferred example of the composition comprises: [0275] 714.3 of hydrolyzed collagen Glow25? [0276] 1.785 of hyaluronic acid [0277] 2.857 of turmeric (Curcuma longa) rhizome extract [0278] 0.003571 of vitamin D3 [0279] 0.002142 of Ginkgo Biloba leaf extract [0280] 281.065 of saline solution.

[0281] In the eighth example, hyaluronic acid is in the form of sodium hyaluronate powder.

[0282] It is herewith disclosed a manufacturing process for the aforementioned composition.

[0283] Hydrolyzed collagen, hyaluronic acid or a pharmaceutically acceptable salt thereof, at least one between the Vitamin C, turmeric rhizome extract, and Ginkgo Biloba leaf extract and vitamin D (when present) are provided in pharmaceutical grade, pure, powders or fine granules.

[0284] The aforementioned elements are mixed together in a mixer by means of any between a substantially automated process or a manual process, up to the moment at which a powder mixture is obtained.

[0285] Subsequently the method comprises the addition of saline solution, in an amount sufficient to obtain the above-disclosed ranges or precise amounts. The addition of saline solution determines obtaining, at first a non-uniform fluid mixture that is subsequently mixed again.

[0286] A further subsequent mixing step therefore takes place, for obtaining a substantially uniform liquid that corresponds to the cosmeceutical composition, with no significant solid residual part that may clog the needle of a syringe. It may be noted that the composition can be injected through needles with gauges down to 33 or 34; solid residual parts may therefore be carefully avoided.

[0287] The so obtained liquid final cosmeceutical composition is then optionally filtered and introduced, preferably in a controlled atmosphere environment, in containers. It is not necessary to use light-attenuating (e.g. brown, green) glass or plastic vessels.

[0288] The composition so obtained may be introduced in a sterile container.

[0289] In an embodiment, which is non-limiting, the container is an injection-ready container.

[0290] An injection-ready container may be provided with a sealing stopper, which is preferably of a self-sealing type. The stopper is pierceable, and in particular is configured and specifically destined to be pierced by a needle, in particular by a needle of a syringe.

[0291] For the purposes of the present disclosure, for self-sealing shall be intended a cap that, once pierced by a needle, in particular a needle of a syringe, and once such needle is extracted, in particular fully extracted, by said cap, closes automatically without any substantial intervention of a user.

[0292] In particular a plastic and/or rubber stopper, suitable to allow the penetration of the needle of a syringe without subsequently spilling any residual liquid that may be present in the composition. The container may be a single-dose vial or a multi-dose vial. The container may be preferably a depyrogenated container.

[0293] Thus, in an embodiment the method comprises collecting a single-dose amount of the liquid final composition into the container. In an alternative embodiment the method comprises collecting a multi-dose amount of the liquid final composition into the container.

[0294] A single-dose amount of the liquid final composition may be, for instance, a dose sufficient to fill substantially a 0.5 ml syringe, or a 1 ml syringe, or a 2 ml syringe, or a 3 ml syringe or a 5 ml syringe.

[0295] A multi-dose amount of the liquid final composition may be, for instance, an amount of 10 ml or more.

[0296] In an embodiment, such container is a syringe, in particular a disposable syringe. This allows the medical doctor or any practitioner to implement the treatment without performing the action of perforating the pierceable sealing cap of the container with an additional syringe.

[0297] The advantages of the composition herewith disclosed are clear in view of the above description. The composition is substantially natural, has proven to be well tolerated also by sensitive patients, the composition, when injected, determines limited side effects as swelling or bleeding or itching. The composition substantially determines an immediate, prolonged, and effective firmness of the skin. In particular, when Ginkgo Biloba leaf extract is present, a temporary effect of redness in the skin is the result of a relevant oxygenation of the skin in the area surrounding the injection site.

[0298] The herewith disclosed composition is easy to manufacture and may be produced in a cost-effective way.

[0299] Several adaptations or additions can be carried out to the object of the present disclosure without for this departing from the scope of protection provided by the annexed claims.