Compounds and uses thereof

Abstract

The present disclosure features compounds useful for the treatment of BAF complex-related disorders.

Claims

1. A compound having the structure of Formula I: ##STR00530## wherein B is ##STR00531## optionally substituted C.sub.2-C.sub.9 heteroaryl, or C.sub.2-C.sub.9 heterocyclyl; R is ##STR00532## wherein R.sup.1 is optionally substituted C.sub.1-C.sub.3 alkyl or optionally substituted C.sub.3-C.sub.6 cycloalkyl; each R.sup.3 and R.sup.5 is, independently, selected from the group consisting of H, optionally substituted C.sub.1-C.sub.6 alkyl, or optionally substituted C.sub.1-C.sub.6 heteroalkyl; R.sup.4 is hydrogen, or optionally substituted C.sub.1-C.sub.6 heteroalkyl; and R.sup.6 is optionally substituted C.sub.6-C.sub.10 aryl or optionally substituted C.sub.2-C.sub.9 heteroaryl or a pharmaceutically acceptable salt thereof.

2. The compound of claim 1, wherein the compound has the structure ##STR00533## wherein R.sup.1 is optionally substituted C.sub.1-C.sub.3 alkyl, or a pharmaceutically acceptable salt thereof.

3. A compound having the structure of any one of compounds ##STR00534## ##STR00535## ##STR00536## and pharmaceutically acceptable salts thereof.

4. A pharmaceutical composition comprising the compound of claim 1 and a pharmaceutically acceptable excipient.

5. The compound of claim 1, wherein both R.sup.3 and R.sup.5 are H.

6. The compound of claim 1, wherein R.sup.4 is H, ##STR00537##

Description

BRIEF DESCRIPTION OF THE DRAWINGS

(1) FIG. 1 is a graph illustrating inhibition of cell proliferation of several cancer cell lines by a BRG1/BRM inhibitor (Compound 87).

(2) FIG. 2 is a graph illustrating inhibition of cell proliferation of uveal melanoma cells by a BRG1/BRM inhibitor (Compound 87), a MEK inhibitor (Selumetinib), and a PKC inhibitor (LXS196).

(3) FIG. 3 is a graph illustrating inhibition of cell proliferation of several cancer cell lines by a BRG1/BRM inhibitor (Compound B).

DETAILED DESCRIPTION

(4) The present disclosure features compounds useful for the inhibition of BRG1 and/or BRM. These compounds may be used to modulate the activity of a BAF complex, for example, for the treatment of a BAF-related disorder, such as cancer. Exemplary compounds described herein include compounds having a structure according to Formula I, Formula IV, Formula V, or Formula VII:

(5) ##STR00308##

(6) or a pharmaceutically acceptable salt thereof.

(7) In some embodiments, the compound, or pharmaceutically acceptable salt thereof, has the structure of any one of compounds 1-241 in Tables 1, 2, 3, and 4.

(8) Other embodiments, as well as exemplary methods for the synthesis of production of these compounds, are described herein.

(9) Pharmaceutical Uses

(10) The compounds described herein are useful in the methods of the invention and, while not bound by theory, are believed to exert their ability to modulate the level, status, and/or activity of a BAF complex, i.e., by inhibiting the activity of the BRG1 and/or BRM proteins within the BAF complex in a mammal. BAF complex-related disorders include, but are not limited to, BRG1 loss of function mutation-related disorders.

(11) An aspect of the present invention relates to methods of treating disorders related to BRG1 loss of function mutations such as cancer (e.g., non-small cell lung cancer, colorectal cancer, bladder cancer, cancer of unknown primary, glioma, breast cancer, melanoma, non-melanoma skin cancer, endometrial cancer, or penile cancer) in a subject in need thereof. Another aspect of the invention relates to methods of treating cancer by administering a compound that selectively inhibits BRM. For example, the compound that selectively inhibits BRM is a compound as described herein. In some embodiments, the compound is administered in an amount and for a time effective to result in one or more (e.g., two or more, three or more, four or more) of: (a) reduced tumor size, (b) reduced rate of tumor growth, (c) increased tumor cell death (d) reduced tumor progression, (e) reduced number of metastases, (f) reduced rate of metastasis, (g) decreased tumor recurrence (h) increased survival of subject, (i) increased progression free survival of subject.

(12) Treating cancer can result in a reduction in size or volume of a tumor. For example, after treatment, tumor size is reduced by 5% or greater (e.g., 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or greater) relative to its size prior to treatment. Size of a tumor may be measured by any reproducible means of measurement. For example, the size of a tumor may be measured as a diameter of the tumor.

(13) Treating cancer may further result in a decrease in number of tumors. For example, after treatment, tumor number is reduced by 5% or greater (e.g., 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or greater) relative to number prior to treatment. Number of tumors may be measured by any reproducible means of measurement, e.g., the number of tumors may be measured by counting tumors visible to the naked eye or at a specified magnification (e.g., 2×, 3×, 4×, 5×, 10×, or 50×).

(14) Treating cancer can result in a decrease in number of metastatic nodules in other tissues or organs distant from the primary tumor site. For example, after treatment, the number of metastatic nodules is reduced by 5% or greater (e.g., 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% or greater) relative to number prior to treatment. The number of metastatic nodules may be measured by any reproducible means of measurement. For example, the number of metastatic nodules may be measured by counting metastatic nodules visible to the naked eye or at a specified magnification (e.g., 2×, 10×, or 50×).

(15) Treating cancer can result in an increase in average survival time of a population of subjects treated according to the present invention in comparison to a population of untreated subjects. For example, the average survival time is increased by more than 30 days (more than 60 days, 90 days, or 120 days). An increase in average survival time of a population may be measured by any reproducible means. An increase in average survival time of a population may be measured, for example, by calculating for a population the average length of survival following initiation of treatment with the compound of the invention. An increase in average survival time of a population may also be measured, for example, by calculating for a population the average length of survival following completion of a first round of treatment with a pharmaceutically acceptable salt of the invention.

(16) Treating cancer can also result in a decrease in the mortality rate of a population of treated subjects in comparison to an untreated population. For example, the mortality rate is decreased by more than 2% (e.g., more than 5%, 10%, or 25%). A decrease in the mortality rate of a population of treated subjects may be measured by any reproducible means, for example, by calculating for a population the average number of disease-related deaths per unit time following initiation of treatment with a pharmaceutically acceptable salt of the invention. A decrease in the mortality rate of a population may also be measured, for example, by calculating for a population the average number of disease-related deaths per unit time following completion of a first round of treatment with a pharmaceutically acceptable salt of the invention.

(17) Exemplary cancers that may be treated by the invention include, but are not limited to, non-small cell lung cancer, small-cell lung cancer, colorectal cancer, bladder cancer, glioma, breast cancer, melanoma, non-melanoma skin cancer, endometrial cancer, esophagogastric cancer, pancreatic cancer, hepatobiliary cancer, soft tissue sarcoma, ovarian cancer, head and neck cancer, renal cell carcinoma, bone cancer, non-hodgkin lymphoma, prostate cancer, embryonal tumor, germ cell tumor, cervical cancer, thyroid cancer, salivary gland cancer, gastrointestinal neuroendocrine tumor, uterine sarcoma, gastrointestinal stromal tumor, CNS cancer, thymic tumor, Adrenocortical carcinoma, appendiceal cancer, small bowel cancer and penile cancer.

(18) Combination Formulations and Uses Thereof

(19) The compounds of the invention can be combined with one or more therapeutic agents. In particular, the therapeutic agent can be one that treats or prophylactically treats any cancer described herein.

(20) Combination Therapies

(21) A compound of the invention can be used alone or in combination with an additional therapeutic agent, e.g., other agents that treat cancer or symptoms associated therewith, or in combination with other types of treatment to treat cancer. In combination treatments, the dosages of one or more of the therapeutic compounds may be reduced from standard dosages when administered alone. For example, doses may be determined empirically from drug combinations and permutations or may be deduced by isobolographic analysis (e.g., Black et al., Neurology 65:S3-S6, 2005). In this case, dosages of the compounds when combined should provide a therapeutic effect.

(22) In some embodiments, the second therapeutic agent is a chemotherapeutic agent (e.g., a cytotoxic agent or other chemical compound useful in the treatment of cancer). These include alkylating agents, antimetabolites, folic acid analogs, pyrimidine analogs, purine analogs and related inhibitors, vinca alkaloids, epipodopyyllotoxins, antibiotics, L-Asparaginase, topoisomerase inhibitors, interferons, platinum coordination complexes, anthracenedione substituted urea, methyl hydrazine derivatives, adrenocortical suppressant, adrenocorticosteroides, progestins, estrogens, antiestrogen, androgens, antiandrogen, and gonadotropin-releasing hormone analog. Also included is 5-fluorouracil (5-FU), leucovorin (LV), irenotecan, oxaliplatin, capecitabine, paclitaxel and doxetaxel. Non-limiting examples of chemotherapeutic agents include alkylating agents such as thiotepa and cyclosphosphamide; alkyl sulfonates such as busulfan, improsulfan and piposulfan; aziridines such as benzodopa, carboquone, meturedopa, and uredopa; ethylenimines and methylamelamines including altretamine, triethylenemelamine, trietylenephosphoramide, triethiylenethiophosphoramide and trimethylolomelamine; acetogenins (especially bullatacin and bullatacinone); a camptothecin (including the synthetic analogue topotecan); bryostatin; callystatin; CC-1065 (including its adozelesin, carzelesin and bizelesin synthetic analogues); cryptophycins (particularly cryptophycin 1 and cryptophycin 8); dolastatin; duocarmycin (including the synthetic analogues, KW-2189 and CB1-TM1); eleutherobin; pancratistatin; a sarcodictyin; spongistatin; nitrogen mustards such as chlorambucil, chlornaphazine, cholophosphamide, estramustine, ifosfamide, mechlorethamine, mechlorethamine oxide hydrochloride, melphalan, novembichin, phenesterine, prednimustine, trofosfamide, uracil mustard; nitrosureas such as carmustine, chlorozotocin, fotemustine, lomustine, nimustine, and ranimnustine; antibiotics such as the enediyne antibiotics (e.g., calicheamicin, especially calicheamicin gammaII and calicheamicin omegaII (see, e.g., Agnew, Chem. Intl. Ed Engl. 33:183-186 (1994)); dynemicin, including dynemicin A; bisphosphonates, such as clodronate; an esperamicin; as well as neocarzinostatin chromophore and related chromoprotein enediyne antiobiotic chromophores), aclacinomysins, actinomycin, authramycin, azaserine, bleomycins, cactinomycin, carabicin, caminomycin, carzinophilin, chromomycinis, dactinomycin, daunorubicin, detorubicin, 6-diazo-5-oxo-L-norleucine, Adriamycin® (doxorubicin, including morpholino-doxorubicin, cyanomorpholino-doxorubicin, 2-pyrrolino-doxorubicin and deoxydoxorubicin), epirubicin, esorubicin, idarubicin, marcellomycin, mitomycins such as mitomycin C, mycophenolic acid, nogalamycin, olivomycins, peplomycin, potfiromycin, puromycin, quelamycin, rodorubicin, streptonigrin, streptozocin, tubercidin, ubenimex, zinostatin, zorubicin; anti-metabolites such as methotrexate and 5-fluorouracil (5-FU); folic acid analogues such as denopterin, methotrexate, pteropterin, trimetrexate; purine analogs such as fludarabine, 6-mercaptopurine, thiamiprine, thioguanine; pyrimidine analogs such as ancitabine, azacitidine, 6-azauridine, carmofur, cytarabine, dideoxyuridine, doxifluridine, enocitabine, floxuridine; androgens such as calusterone, dromostanolone propionate, epitiostanol, mepitiostane, testolactone; anti-adrenals such as aminoglutethimide, mitotane, trilostane; folic acid replenisher such as frolinic acid; aceglatone; aldophosphamide glycoside; aminolevulinic acid; eniluracil; amsacrine; bestrabucil; bisantrene; edatraxate; defofamine; demecolcine; diaziquone; elfomithine; elliptinium acetate; an epothilone; etoglucid; gallium nitrate; hydroxyurea; lentinan; lonidainine; maytansinoids such as maytansine and ansamitocins; mitoguazone; mitoxantrone; mopidanmol; nitraerine; pentostatin; phenamet; pirarubicin; losoxantrone; podophyllinic acid; 2-ethylhydrazide; procarbazine; PSK® polysaccharide complex (JHS Natural Products, Eugene, Oreg.); razoxane; rhizoxin; sizofuran; spirogermanium; tenuazonic acid; triaziquone; 2,2′,2″-trichlorotriethylamine; trichothecenes (especially T-2 toxin, verracurin A, roridin A and anguidine); urethan; vindesine; dacarbazine; mannomustine; mitobronitol; mitolactol; pipobroman; gacytosine; arabinoside (“Ara-C”); cyclophosphamide; thiotepa; taxoids, e.g., Taxol® paclitaxel (Bristol-Myers Squibb Oncology, Princeton, N.J.), ABraxane®, cremophor-free, albumin-engineered nanoparticle formulation of paclitaxel (American Pharmaceutical Partners, Schaumberg, Ill.), and Taxotere® doxetaxel (Rhone-Poulenc Rorer, Antony, France); chloranbucil; Gemzar® gemcitabine; 6-thioguanine; mercaptopurine; methotrexate; platinum coordination complexes such as cisplatin, oxaliplatin and carboplatin; vinblastine; platinum; etoposide (VP-16); ifosfamide; mitoxantrone; vincristine; Navelbine® vinorelbine; novantrone; teniposide; edatrexate; daunomycin; aminopterin; xeloda; ibandronate; irinotecan (e.g., CPT-11); topoisomerase inhibitor RFS 2000; difluoromethylornithine (DMFO); retinoids such as retinoic acid; capecitabine; and pharmaceutically acceptable salts, acids or derivatives of any of the above. Two or more chemotherapeutic agents can be used in a cocktail to be administered in combination with the first therapeutic agent described herein. Suitable dosing regimens of combination chemotherapies are known in the art and described in, for example, Saltz et al. (1999) Proc ASCO 18:233a and Douillard et al. (2000) Lancet 355:1041-7.

(23) In some embodiments, the second therapeutic agent is a therapeutic agent which is a biologic such a cytokine (e.g., interferon or an interleukin (e.g., IL-2)) used in cancer treatment. In some embodiments the biologic is an anti-angiogenic agent, such as an anti-VEGF agent, e.g., bevacizumab (Avastin®). In some embodiments the biologic is an immunoglobulin-based biologic, e.g., a monoclonal antibody (e.g., a humanized antibody, a fully human antibody, an Fc fusion protein or a functional fragment thereof) that agonizes a target to stimulate an anti-cancer response, or antagonizes an antigen important for cancer. Such agents include Rituxan (Rituximab); Zenapax (Daclizumab); Simulect (Basiliximab); Synagis (Palivizumab); Remicade (Infliximab); Herceptin (Trastuzumab); Mylotarg (Gemtuzumab ozogamicin); Campath (Alemtuzumab); Zevalin (Ibritumomab tiuxetan); Humira (Adalimumab); Xolair (Omalizumab); Bexxar (Tositumomab-I-131); Raptiva (Efalizumab); Erbitux (Cetuximab); Avastin (Bevacizumab); Tysabri (Natalizumab); Actemra (Tocilizumab); Vectibix (Panitumumab); Lucentis (Ranibizumab); Soliris (Eculizumab); Cimzia (Certolizumab pegol); Simponi (Golimumab); Ilaris (Canakinumab); Stelara (Ustekinumab); Arzerra (Ofatumumab); Prolia (Denosumab); Numax (Motavizumab); ABThrax (Raxibacumab); Benlysta (Belimumab); Yervoy (Ipilimumab); Adcetris (Brentuximab Vedotin); Perjeta (Pertuzumab); Kadcyla (Ado-trastuzumab emtansine); and Gazyva (Obinutuzumab). Also included are antibody-drug conjugates.

(24) The second agent may be a therapeutic agent which is a non-drug treatment. For example, the second therapeutic agent is radiation therapy, cryotherapy, hyperthermia and/or surgical excision of tumor tissue.

(25) The second agent may be a checkpoint inhibitor. In one embodiment, the inhibitor of checkpoint is an inhibitory antibody (e.g., a monospecific antibody such as a monoclonal antibody). The antibody may be, e.g., humanized or fully human. In some embodiments, the inhibitor of checkpoint is a fusion protein, e.g., an Fc-receptor fusion protein. In some embodiments, the inhibitor of checkpoint is an agent, such as an antibody, that interacts with a checkpoint protein. In some embodiments, the inhibitor of checkpoint is an agent, such as an antibody, that interacts with the ligand of a checkpoint protein. In some embodiments, the inhibitor of checkpoint is an inhibitor (e.g., an inhibitory antibody or small molecule inhibitor) of CTLA-4 (e.g., an anti-CTLA4 antibody such as ipilimumab/Yervoy or tremelimumab). In some embodiments, the inhibitor of checkpoint is an inhibitor (e.g., an inhibitory antibody or small molecule inhibitor) of PD-1 (e.g., nivolumab/Opdivo®; pembrolizumab/Keytruda®; pidilizumab/CT-011). In some embodiments, the inhibitor of checkpoint is an inhibitor (e.g., an inhibitory antibody or small molecule inhibitor) of PDL1 (e.g., MPDL3280A/RG7446; MED14736; MSB0010718C; BMS 936559). In some embodiments, the inhibitor of checkpoint is an inhibitor (e.g., an inhibitory antibody or Fc fusion or small molecule inhibitor) of PDL2 (e.g., a PDL2/Ig fusion protein such as AMP 224). In some embodiments, the inhibitor of checkpoint is an inhibitor (e.g., an inhibitory antibody or small molecule inhibitor) of B7-H3 (e.g., MGA271), B7-H4, BTLA, HVEM, TIM3, GAL9, LAGS, VISTA, KIR, 2B4, CD160, CGEN-15049, CHK 1, CHK2, A2aR, B-7 family ligands, or a combination thereof.

(26) In any of the combination embodiments described herein, the first and second therapeutic agents are administered simultaneously or sequentially, in either order. The first therapeutic agent may be administered immediately, up to 1 hour, up to 2 hours, up to 3 hours, up to 4 hours, up to 5 hours, up to 6 hours, up to 7 hours, up to, 8 hours, up to 9 hours, up to 10 hours, up to 11 hours, up to 12 hours, up to 13 hours, 14 hours, up to hours 16, up to 17 hours, up 18 hours, up to 19 hours up to 20 hours, up to 21 hours, up to 22 hours, up to 23 hours up to 24 hours or up to 1-7, 1-14, 1-21 or 1-30 days before or after the second therapeutic agent

(27) Pharmaceutical Compositions

(28) The compounds of the invention are preferably formulated into pharmaceutical compositions for administration to a mammal, preferably, a human, in a biologically compatible form suitable for administration in vivo. Accordingly, in an aspect, the present invention provides a pharmaceutical composition comprising a compound of the invention in admixture with a suitable diluent, carrier, or excipient.

(29) The compounds of the invention may be used in the form of the free base, in the form of salts, solvates, and as prodrugs. All forms are within the scope of the invention. In accordance with the methods of the invention, the described compounds or salts, solvates, or prodrugs thereof may be administered to a patient in a variety of forms depending on the selected route of administration, as will be understood by those skilled in the art. The compounds of the invention may be administered, for example, by oral, parenteral, buccal, sublingual, nasal, rectal, patch, pump, or transdermal administration and the pharmaceutical compositions formulated accordingly. Parenteral administration includes intravenous, intraperitoneal, subcutaneous, intramuscular, transepithelial, nasal, intrapulmonary, intrathecal, rectal, and topical modes of administration. Parenteral administration may be by continuous infusion over a selected period of time.

(30) A compound of the invention may be orally administered, for example, with an inert diluent or with an assimilable edible carrier, or it may be enclosed in hard or soft shell gelatin capsules, or it may be compressed into tablets, or it may be incorporated directly with the food of the diet. For oral therapeutic administration, a compound of the invention may be incorporated with an excipient and used in the form of ingestible tablets, buccal tablets, troches, capsules, elixirs, suspensions, syrups, and wafers. A compound of the invention may also be administered parenterally. Solutions of a compound of the invention can be prepared in water suitably mixed with a surfactant, such as hydroxypropylcellulose. Dispersions can also be prepared in glycerol, liquid polyethylene glycols, DMSO, and mixtures thereof with or without alcohol, and in oils. Under ordinary conditions of storage and use, these preparations may contain a preservative to prevent the growth of microorganisms. Conventional procedures and ingredients for the selection and preparation of suitable formulations are described, for example, in Remington's Pharmaceutical Sciences (2003, 20th ed.) and in The United States Pharmacopeia: The National Formulary (USP 24 NF19), published in 1999. The pharmaceutical forms suitable for injectable use include sterile aqueous solutions or dispersions and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersions. In all cases the form must be sterile and must be fluid to the extent that may be easily administered via syringe. Compositions for nasal administration may conveniently be formulated as aerosols, drops, gels, and powders. Aerosol formulations typically include a solution or fine suspension of the active substance in a physiologically acceptable aqueous or non-aqueous solvent and are usually presented in single or multidose quantities in sterile form in a sealed container, which can take the form of a cartridge or refill for use with an atomizing device. Alternatively, the sealed container may be a unitary dispensing device, such as a single dose nasal inhaler or an aerosol dispenser fitted with a metering valve which is intended for disposal after use. Where the dosage form comprises an aerosol dispenser, it will contain a propellant, which can be a compressed gas, such as compressed air or an organic propellant, such as fluorochlorohydrocarbon. The aerosol dosage forms can also take the form of a pump-atomizer. Compositions suitable for buccal or sublingual administration include tablets, lozenges, and pastilles, where the active ingredient is formulated with a carrier, such as sugar, acacia, tragacanth, gelatin, and glycerine. Compositions for rectal administration are conveniently in the form of suppositories containing a conventional suppository base, such as cocoa butter. A compound described herein may be administered intratumorally, for example, as an intratumoral injection. Intratumoral injection is injection directly into the tumor vasculature and is specifically contemplated for discrete, solid, accessible tumors. Local, regional, or systemic administration also may be appropriate. A compound described herein may advantageously be contacted by administering an injection or multiple injections to the tumor, spaced for example, at approximately, 1 cm intervals. In the case of surgical intervention, the present invention may be used preoperatively, such as to render an inoperable tumor subject to resection. Continuous administration also may be applied where appropriate, for example, by implanting a catheter into a tumor or into tumor vasculature.

(31) The compounds of the invention may be administered to an animal, e.g., a human, alone or in combination with pharmaceutically acceptable carriers, as noted herein, the proportion of which is determined by the solubility and chemical nature of the compound, chosen route of administration, and standard pharmaceutical practice.

(32) Dosages

(33) The dosage of the compounds of the invention, and/or compositions comprising a compound of the invention, can vary depending on many factors, such as the pharmacodynamic properties of the compound; the mode of administration; the age, health, and weight of the recipient; the nature and extent of the symptoms; the frequency of the treatment, and the type of concurrent treatment, if any; and the clearance rate of the compound in the animal to be treated. One of skill in the art can determine the appropriate dosage based on the above factors. The compounds of the invention may be administered initially in a suitable dosage that may be adjusted as required, depending on the clinical response. In general, satisfactory results may be obtained when the compounds of the invention are administered to a human at a daily dosage of, for example, between 0.05 mg and 3000 mg (measured as the solid form). Dose ranges include, for example, between 10-1000 mg (e.g., 50-800 mg). In some embodiments, 50, 100, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950, or 1000 mg of the compound is administered.

(34) Alternatively, the dosage amount can be calculated using the body weight of the patient. For example, the dose of a compound, or pharmaceutical composition thereof, administered to a patient may range from 0.1-50 mg/kg (e.g., 0.25-25 mg/kg). In exemplary, non-limiting embodiments, the dose may range from 0.5-5.0 mg/kg (e.g., 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0, 4.5, or 5.0 mg/kg) or from 5.0-20 mg/kg (e.g., 5.5, 6.0, 6.5, 7.0, 7.5, 8.0, 8.5, 9.0, 9.5, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20 mg/kg).

EXAMPLES

Example 1. Preparation of 3-(dimethylsulfamoyl)-N-[2-oxo-2-[(4-phenylthiazol-2-yl)amino]ethyl]benzamide (Compound 13)

(35) ##STR00309##

Step 1: Preparation of tert-butyl N-[2-oxo-2-[(4-phenylthiazol-2-yl)amino]ethyl]carbamate (i-1a)

(36) ##STR00310##

(37) To a solution of 2-(tert-butoxycarbonylamino)acetic acid (1 g, 5.71 mmol) in dimethylformamide (DMF) (20 mL) was added 1-[Bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxide hexafluorophosphate (HATU) (2.39 g, 6.28 mmol) and N-Ethyl-N-(propan-2-yl)propan-2-amine (DIPEA) (2.21 g, 17.13 mmol). The solution was stirred for 10 minutes and then 4-phenylthiazol-2-amine (1.01 g, 5.71 mmol) was added. The solution was stirred at 30° C. for 12 hrs. The reaction solution was poured into water (200 mL) and then extracted with EtOAc (200 mL). The organic layer was washed with aqueous citric acid (100 mL*5), brine (200 mL) and then dried over Na.sub.2SO.sub.4. The solution was concentrated in vacuum. The residue was purified through column chromatography (SiO.sub.2, PE/EtOAc=20/1˜3/1) to give intermediate i-1a as a yellow solid.

(38) .sup.1H NMR (400 MHz, CDCl3) δ=10.08 (brs, 1H), 7.83-7.81 (m, 2H), 7.45-7.41 (m, 2H), 7.36-7.34 (m, 1H), 7.28 (s, 1H), 7.17 (s, 1H), 4.04 (s, 2H), 1.51 (s, 9H) ppm.

(39) LCMS (ESI) m/z: [M+H].sup.+=333.9.

Step 2: Preparation of 2-amino-N-(4-phenylthiazol-2-yl)acetamide hydrochloride salt (i-1b)

(40) ##STR00311##

(41) To a solution of intermediate i-1a (100 mg, 284.94 μmol) in dioxane (5 mL) was added HCl/dioxane (4 M, 5 mL), then the solution was stirred at 30° C. for 1 hr. The reaction solution was concentrated in vacuum to give intermediate i-1b (HCl salt) as a white solid, which was used for the next step directly.

(42) LCMS (ESI) m/z: [M+H].sup.+=233.9.

Step 3: Preparation of 3-(dimethylsulfamoyl)-N-[2-oxo-2-[(4-phenylthiazol-2-yl)amino]ethyl]benzamide (Compound 13)

(43) ##STR00312##

(44) To a solution of 3-(dimethylsulfamoyl)benzoic acid (75.72 mg, 330.31 μmol) in DCM (10 mL) was added HATU (125.59 mg, 330.31 μmol) and DIPEA (155.24 mg, 1.20 mmol). The solution was stirred for 10 min and then intermediate i-1 b (81 mg, 300.28 μmol) was added. The solution was stirred at 30° C. for 12 hr. The reaction solution was poured into water (50 mL) and extracted with EtOAc (100 mL). The organic layer was washed with brine (50 mL), dried (Na.sub.2SO.sub.4) and concentrated in vacuum. To the residue was added MeOH (20 mL) and stirred for 20 min, a white solid was formed. The solid was filtered and dried in vacuum to give compound 13 as a white solid.

(45) LCMS (ESI) m/z: [M+H].sup.+=444.9.

(46) .sup.1H NMR (400 MHz, DMSO-d6) δ=12.49 (s, 1H), 9.32 (t, J=5.6 Hz, 1H), 8.27-8.25 (m, 2H), 7.96-7.90 (m, 3H), 7.81-7.80 (m, 1H), 7.64 (s, 1H), 7.46-7.42 (m, 2H), 7.33-7.30 (m, 1H), 4.23 (d, J=5.6 Hz, 2H), 2.65 (s, 6H) ppm.

Example 2. Preparation of Compound (S)—N-(4-(methylthio)-1-oxo-1-((4-phenylthiazol-2-yl)amino)butan-2-yl)benzamide (Compound 42)

(47) ##STR00313##

Step 1: Preparation of (S)-tert-butyl (4-(methylthio)-1-oxo-1-((4-phenylthiazol-2-yl)amino)butan-2-yl)carbamate (Intermediate i-2a)

(48) ##STR00314##

(49) To a solution of (2S)-2-(tert-butoxycarbonylamino)-4-methylsulfanyl-butanoic acid (1 g, 4.01 mmol) in DCM (20 mL) was added 2-ethoxy-1-ethoxycarbonyl-1,2-dihydroquinoline (EEDQ) (1.24 g, 5.01 mmol) and then 4-phenylthiazol-2-amine (589.05 mg, 3.34 mmol) was added. The mixture was stirred at 28° C. for 3 hr. The reaction mixture was diluted with EtOAc (400 mL). The organic layer was washed with brine (40 mL), dried over Na.sub.2SO.sub.4, filtered and concentrated under reduced pressure to give a residue. The residue was purified by column chromatography (SiO.sub.2, Petroleum ether/Ethyl acetate=50/1 to 8/1) to give intermediate i-2a as a white solid.

(50) 1H NMR (400 MHz, METHANOL-d4) δ 7.89 (d, J=7.6 Hz, 2H), 7.43-7.35 (m, 3H), 7.30-7.27 (m, 1H), 4.41-4.32 (m, 1H), 2.62-2.55 (m, 2H), 2.20-2.11 (m, 4H), 2.00-1.94 (m, 1H), 1.46-1.39 (m, 9H) ppm.

(51) LCMS (ESI) m/z: [M+H].sup.+=408.2.

Step 2: Preparation of (S)-2-amino-4-(methylthio)-N-(4-phenylthiazol-2-yl)butanamide trifluoroacetate salt (Intermediate i-2b)

(52) ##STR00315##

(53) To a solution of intermediate i-2a (200 mg, 490.74 μmol) in DCM (5 mL) was added TFA (0.25 mL). The mixture was stirred at 28° C. for 2 hr. To the reaction solution was added a.q NaHCO.sub.3 to adjust pH to 7 and then extracted with EtOAc (100 mL*2). The combined organic layers were washed with brine (20 mL), dried over Na.sub.2SO.sub.4, filtered and concentrated under reduced pressure to give a residue. The residue was purified by prep-HPLC (TFA condition; column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 15%-45% 12 min) and lyophilized to give the TFA salt of intermediate i-2b as a white solid.

(54) 1H NMR (400 MHz, METHANOL-d4) δ 7.91-7.89 (m, 2H), 7.46 (s, 1H), 7.42-7.38 (m, 2H), 7.32-7.31 (m, 1H), 4.27-4.23 (m, 1H), 2.67-2.63 (m, 2H), 2.33-2.22 (m, 2H), 2.14 (s, 3H) ppm.

(55) LCMS (ESI) m/z: [M+H].sup.+=308.1.

Step 3: Preparation of (S)—N-(4-(methylthio)-1-oxo-1-((4-phenylthiazol-2-yl)amino)butan-2-yl)benzamide (Compound 42)

(56) ##STR00316##

(57) To a solution of benzoic acid (43.70 mg, 357.80 μmol) in DCM (2 mL) was added EEDQ (120.66 mg, 487.91 μmol) and then intermediate i-2b (100 mg, 325.27 μmol) was added. The mixture was stirred at 28° C. for 3 hr. The reaction mixture was diluted with EA (150 mL), washed with 10% aqueous citric acid (50 mL*4), dried over Na.sub.2SO.sub.4, filtered and concentrated under reduced pressure to give a residue. The residue was purified by prep-HPLC (TFA condition; column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 45%-75% min) and lyophilized to give compound 42 as a white solid.

(58) 1H NMR (400 MHz, METHANOL-d4) δ 7.91-7.88 (m, 4H), 7.60-7.51 (m, 1H), 7.50-7.48 (m, 2H), 7.38-7.36 (m, 3H), 7.29-7.27 (m, 1H), 4.93-4.89 (m, 1H), 2.70-2.63 (m, 2H), 2.28-2.21 (m, 2H), 2.13 (s, 3H) ppm.

(59) LCMS (ESI) m/z: [M+H].sup.+=412.2.

(60) Chiral HPLC: AD-3S_3_40_3ML_12MIN_T35.M, 4.866 min.

Example 3. Preparation of (S)-3-(N,N-dimethylsulfamoyl)-N-(4-(methylthio)-1-oxo-1-((4-phenylthiazol-2-yl)amino)butan-2-yl)benzamide (Compound 24)

(61) ##STR00317##

(62) To a solution of 3-(dimethylsulfamoyl)benzoic acid (59.66 mg, 260.22 μmol) in DCM (2 mL) was added EEDQ (96.52 mg, 390.33 μmol) and then (2S)-2-amino-4-methylsulfanyl-N-(4-phenylthiazol-2-yl)butanamide (80 mg, 260.22 μmol) was added. The mixture was stirred at 28° C. for 2 hr. The reaction mixture was diluted with EtOAc (150 mL). The organic layer was washed with brine (50 mL), dried over Na.sub.2SO.sub.4, filtered and concentrated under reduced pressure to give a residue. The residue was purified by column chromatography (SiO.sub.2, Petroleum ether/Ethyl acetate=50/1 to 1:1) and lyophilized to give compound 24 as a white solid.

(63) 1H NMR (400 MHz, DMSO-d6) δ 12.55 (s, 1H), 9.10 (d, J=6.8 Hz, 1H), 8.28-8.26 (m, 2H), 7.92-7.89 (m, 3H), 7.80-7.76 (m, 1H), 7.64 (s, 1H), 7.45-7.41 (m, 2H), 7.36-7.30 (m, 1H), 4.83-4.76 (m, 1H), 2.65 (s, 6H), 2.61-2.55 (m, 2H), 2.21-2.16 (m, 2H), 2.10 (s, 3H) ppm.

(64) LCMS (ESI) m/z: [M+H].sup.+=519.2.

Example 4. Preparation of (S)-3-(N,N-dimethylsulfamoyl)-N-(1-oxo-1-((4-phenylthiazol-2-yl)amino)propan-2-yl)benzamide (Compound 12)

(65) ##STR00318##

Step 1: Preparation of (S)-tert-butyl (1-oxo-1-((4-phenylthiazol-2-yl)amino)propan-2-yl)carbamate (Intermediate 4a)

(66) ##STR00319##

(67) To a solution of (2S)-2-(tert-butoxycarbonylamino)propanoic acid (200 mg, 1.06 mmol) in THF (5 mL) was added EEDQ (392.09 mg, 1.59 mmol), then 4-phenylthiazol-2-amine (186.29 mg, 1.06 mmol) was added to the mixture. The mixture was stirred at 25° C. for 12 hr. Citric acid (10%) 80 mL (40*2) was added and the reaction mixture was extracted with EtOAc (100 mL*2). The combined organic layers were washed with brine (20 mL), dried over Na.sub.2SO.sub.4, filtered and concentrated under reduced pressure to give a residue. The residue was purified by column chromatography (SiO.sub.2, Petroleum ether/Ethyl acetate=50/1 to 5:1) to give intermediate i-4a as a white solid.

(68) .sup.1HNMR (400 MHz, DMSO-d6) δ 12.29 (s, 1H), 7.96-7.84 (m, 2H), 7.63 (s, 1H), 7.49-7.39 (m, 2H), 7.35-7.30 (m, 1H), 7.25 (d, J=6.8 Hz, 1H), 4.31-4.20 (m, 1H), 1.38 (s, 7H), 1.28 (d, J=7.2 Hz, 4H) ppm.

(69) LCMS (ESI) m/z: [M+H].sup.+=347.9.

(70) SFC HPLC: AD-3-MeOH(DEA)-5-40-3 mL-35T.I, 1.584 min.

Step 2: Preparation of (S)-2-amino-N-(4-phenylthiazol-2-yl)propanamide (Intermediate i-4b)

(71) ##STR00320##

(72) To a solution of intermediate i-4a (300 mg, 863.48 μmol) in DCM (5 mL) was added TFA (0.5 mL). The mixture was stirred at 25° C. for 2 hr. The reaction solution was poured into water (20 mL) and adjusted pH to 7 with NaHCO.sub.3. The reaction mixture was extracted with EA (100 mL*2). The combined organic layers were washed with brine (40 mL), dried over Na.sub.2SO.sub.4, filtered and concentrated under reduced pressure to give intermediate i-4b as a white solid which was used to the next step without further purification.

(73) .sup.1HNMR (400 MHz, DMSO-d6) δ 7.93-7.87 (m, 2H), 7.60 (s, 1H), 7.46-7.39 (m, 2H), 7.35-7.28 (m, 1H), 5.46 (s, 2H), 3.59-3.57 (m, 1H), 3.33 (s, 3H), 1.23 (d, J=6.8 Hz, 3H) ppm.

(74) LCMS (ESI) m/z: [M+H].sup.+=248.0.

(75) SFC HPLC: OD-3-MeOH(DEA)-5-40-3 mL-35T.I, 1.708 min.

Step 3: Preparation of (S)-3-(N,N-dimethylsulfamoyl)-N-(1-oxo-1-((4-phenylthiazol-2-yl)amino)propan-2-yl)benzamide (Compound 12)

(76) ##STR00321##

(77) To a solution of 3-(dimethylsulfamoyl)benzoic acid (92.70 mg, 404.34 μmol) in DCM (3 mL) was added HATU (169.12 mg, 444.78 μmol) and DIPEA (156.77 mg, 1.21 mmol, 211.29 μL), then intermediate i-4b (100 mg, 404.34 μmol) was added to the mixture. The mixture was stirred at 25° C. for 2 hr. The reaction mixture was concentrated under reduced pressure to give a solid. To the solid was added MeOH (80 mL) and stirred for 10 min and then filtered, the solid was collected by filtration and dried in vacuum to give compound 12 as a white solid.

(78) .sup.1HNMR (400 MHz, DMSO-d6) δ 12.48 (s, 1H), 9.13 (d, J=6.4 Hz, 1H), 8.30-8.23 (m, 2H), 7.92-7.89 (m, 3H), 7.80-7.76 (m, 1H), 7.64 (s, 1H), 7.46-7.41 (m, 2H), 7.36-7.29 (m, 1H), 4.76-4.69 (m, 1H), 2.65 (s, 6H), 1.49 (d, J=7.2 Hz, 3H) ppm.

(79) LCMS (ESI) m/z: [M+H].sup.+=459.0.

(80) SFC HPLC: OD-3-EtOH(DEA)-5-40-3 mL-35T.Ic, 2.455 min.

Example 5. Preparation of 3-(dimethylsulfamoyl)-N-[2-oxo-2-(3-phenyl anilino)ethyl]benzamide (Compound 40)

(81) ##STR00322##

Step 1: Preparation of tert-butyl N-[2-oxo-2-(3-phenylanilino)ethyl]carbamate (Intermediate i-5a)

(82) ##STR00323##

(83) To a solution of 2-(tert-butoxycarbonylamino)acetic acid (113.87 mg, 650.03 μmol) in DCM (3 mL) was added DIPEA (229.12 mg, 1.77 mmol) and HATU (337.04 mg, 886.41 μmol). The mixture was stirred at 25° C. for 10 min. 3-phenylaniline (100.00 mg, 590.94 μmol) was added and the mixture was stirred at 25° C. for 2 hr. The reaction mixture was quenched by addition water (10 mL), and then extracted with ethyl acetate (10 mL*2). The combined organic layers were washed with water (10 mL*2), dried over Na.sub.2SO.sub.4, filtered and concentrated under reduced pressure to give a crude product. To the residue was added a solvent (EA:PE=1:5, v/v, 10 mL) and stirred for 10 min then filtered, the solid was dried in vacuum to give Intermediate i-5a as a pale yellow solid.

(84) LCMS (ESI) m/z: [M+Na].sup.+=349.2.

Step 2: Preparation of 2-amino-N-(3-phenylphenyl)acetamide (Intermediate i-5b) trifluoroactetate salt

(85) ##STR00324##

(86) To a solution of Intermediate i-5a (70.11 mg, 201.48 μmol) in DCM (3 mL) was added TFA (150.00 μL). The mixture was stirred at 25° C. for 1 hr. The reaction mixture was concentrated to dryness to give a residue, which was slurried with methyl t-butyl ether (5 mL) and the solid collected by filtration, and dried in vacuum to afford intermediate i-5b (TFA salt) as a light yellow solid, which was used in next step without further purification.

(87) LCMS (ESI) m/z: [M+H].sup.+=227.2.

Step 3: Preparation of 3-(dimethylsulfamoyl)-N-[2-oxo-2-(3-phenyl anilino)ethyl]benzamide (Compound 40)

(88) ##STR00325##

(89) To a solution of 3-(dimethylsulfamoyl)benzoic acid (45.59 mg, 198.87 μmol) in DCM (3 mL) was added DIPEA (77.11 mg, 596.62 μmol) and HATU (113.43 mg, 298.31 μmol). The mixture was stirred at 25° C. for 10 min and then intermediate i-5b was added and the mixture was stirred at 25° C. for 2 hr. The reaction mixture was concentrated in vacuo to give a residue. To the residue was added a mixture of methanol:water=1:1, v/v, (4 mL) and stirred for 10 min, then filtered, the filter cake was washed with methanol (2 mL) and then lyophilized to give compound 40 as a white solid.

(90) .sup.1H NMR (400 MHz, DMSO-d6) δ 10.22 (s, 1H), 9.24 (m, 1H), 8.19-8.32 (m, 2H), 7.90-8.00 (m, 2H), 7.73-7.86 (m, 1H), 7.56-7.65 (m, 3H), 7.32-7.51 (m, 5H), 4.14 (m, 2H), 2.63-2.68 (m, 6H) ppm; LCMS (ESI) m/z: [M+H].sup.+=438.2.

Example 6. Preparation of 3-(methylsulfamoyl)-N-[2-oxo-2-[(4-phenyl thiazol-2-yl)amino]ethyl]benzamide (Compound 26)

(91) ##STR00326##

Step 1: Preparation of 2-amino-N-(4-phenylthiazol-2-yl)acetamide (Intermediate 6a) trifluoroactetate salt

(92) ##STR00327##

(93) To a solution of tert-butyl N-[2-oxo-2-[(4-phenylthiazol-2-yl)amino]ethyl]carbamate (500 mg, 1.50 mmol) in DCM (3 mL) was added TFA (2.22 mL). The mixture was stirred at 25° C. for 1 h. The reaction mixture was evaporated to dryness. To the residue was added MTBE/PE=(2/1, 30 mL) and stirred for 10 min, then filtered to give a solid which was dried in vacuum to give intermediate i-6a (TFA salt) as a yellow solid.

(94) LCMS (ESI) m/z: [M+H].sup.+=234.2.

Step 2: Preparation of 3-(methylsulfamoyl)-N-[2-oxo-2-[(4-phenylthiazol-2-yl)amino]ethyl]benzamide (Compound 26)

(95) ##STR00328##

(96) To a mixture of 3-(methylsulfamoyl)benzoic acid (46.13 mg, 214.33 μmol) in DCM (2 mL) was added HATU (89.64 mg, 235.75 μmol) and DIPEA (138.5 mg, 1.07 mmol) at 25° C. The mixture was stirred at 25° C. for 10 min and then intermediate 6a (50 mg, 143.96 μmol) was added and stirred for 1 h. The reaction mixture was evaporated to dryness to give the crude product. To the residue was added MeOH (30 mL) and stirred for 10 min, then filtered and the filter cake was washed with MeOH (30 mL). The solid was dried in vacuo to give compound 26 as a white solid.

(97) .sup.1HNMR (400 MHz, DMSO-d.sub.6) δ=12.47 (brs, 1H), 9.25-9.23 (m, 1H), 8.31 (s, 1H), 8.16 (d, J=7.9 Hz, 1H), 7.96 (d, J=7.8 Hz, 1H), 7.90 (m, 2H), 7.76-7.74 (m, 1H), 7.64 (s, 1H), 7.00-7.58 (m, 1H), 7.46-7.40 (m, 2H), 7.32-7.29 (m, 1H), 4.23 (d, J=5.6 Hz, 2H), 2.43 (m, 3H) ppm; LCMS (ESI) m/z: [M+H].sup.+=431.1.

Example 7. Preparation of Compound N-(2-oxo-2-((4-phenylthiazol-2-yl)amino)ethyl)-3-sulfamoylbenzamide (Compound 28)

(98) ##STR00329##

Step 1: Preparation of N-(2-oxo-2-((4-phenylthiazol-2-yl)amino)ethyl)-3-sulfamoylbenzamide (Compound 28)

(99) ##STR00330##

(100) To a solution of 2-amino-N-(4-phenylthiazol-2-yl)acetamide (50 mg, 143.96 μmol) in DCM (2.0 mL) was added 3-sulfamoylbenzoic acid (28.97 mg, 143.96 μmol) and DIPEA (74.42 mg, 575.85 μmol, 100.30 μL). Then HATU (54.74 mg, 143.96 μmol) was added to the reaction. The mixture was stirred at 25° C. for 2 hr. Water (80.0 mL) was added and the reaction mixture was extracted with EtOAc (80.0 mL). The combined organic layers were washed with brine (20.0 mL), dried over Na.sub.2SO.sub.4, filtered and concentrated under reduced pressure to give a residue. To the residue was added DCM (80.0 mL) and then stirred for 10 min. The solid was filtered and dried in vacuum to give compound 28 as an off-white solid.

(101) .sup.1HNMR (400 MHz, DMSO-d6) δ=12.47 (s, 1H), 9.21-9.18 (m, 1H), 8.37 (s, 1H), 8.12 (d, J=7.6 Hz, 1H), 8.01 (d, J=8.0 Hz, 1H), 7.91-7.90 (m, 2H), 7.74-7.72 (m, 1H), 7.64 (s, 1H), 7.48-7.42 (m, 4H), 7.33-7.30 (m, 1H), 4.23 (d, J=5.6 Hz, 2H) ppm.

(102) LCMS (ESI) m/z: [M-t-Bu].sup.+=417.2.

Example 8. Preparation of 3-methylsulfonyl-N-[2-oxo-2-[(4-phenylthiazol-2-yl)amino]ethyl]benzamide (Compound 23)

(103) ##STR00331##

Step 1: Preparation of 2-amino-N-(4-phenylthiazol-2-yl)acetamide (Intermediate 8a) trifluoracetate salt

(104) ##STR00332##

(105) To a solution of tert-butyl N-[2-oxo-2-[(4-phenylthiazol-2-yl)amino]ethyl]carbamate (500 mg, 1.50 mmol) in DCM (3 mL) was added TFA (2.22 mL). The mixture was stirred at 25° C. for 1 hr. The reaction mixture was evaporated to dryness. To the residue was added MTBE/PE=(2/1, 30 mL) and stirred for 10 min then filtered to give a solid which was dried in vacuum to give intermediate i-8a (TFA salt) as a yellow solid.

(106) LCMS (ESI) m/z: [M+H].sup.+=234.2.

Step 2: Preparation of 3-methylsulfonyl-N-[2-oxo-2-[(4-phenylthiazol-2-yl)amino]ethyl]benzamide (Compound 23)

(107) ##STR00333##

(108) To a solution of 3-methylsulfonylbenzoic acid (42.91 mg, 214.32 μmol) in DCM (2 mL) were added HATU (89.64 mg, 235.75 μmol) and DIPEA (138.5 mg, 1.07 mmol) at 25° C. The mixture was stirred for 10 min and then intermediate i-8a (50 mg, 143.96 μmol) was added and the mixture was stirred at 25° C. for 1 hr. The reaction mixture was evaporated to dryness. To the residue was added 30 mL MeOH and stirred for 10 min, a white solid formed, the solid was filtered off and dried in vacuum to give compound 23 as an off-white solid.

(109) .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 12.44 (br s, 1H), 9.26-9.25 (m, 1H), 8.44 (s, 1H), 8.23 (d, J=7.6 Hz, 1H), 8.10 (d, J=7.6 Hz, 1H), 7.91-7.89 (m, 2H), 7.80-7.78 (m, 1H), 7.63 (s, 1H), 7.45-7.41 (m, 2H), 7.32-7.30 (m, 1H), 4.24 (d, J=6.0 Hz, 2H), 3.27 (s, 3H) ppm.

(110) LCMS (ESI) m/z: [M+H].sup.+=416.1.

Example 9. Preparation of 3-(dimethylsulfamoyl)-N-[2-oxo-2-[[4-(2-pyridyl)thiazol-2-yl]amino]ethyl]benzamide (Compound 32)

(111) ##STR00334##

Step 1: Preparation of 2-amino-N-[4-(2-pyridyl)thiazol-2-yl]acetamide (Intermediate 9a) trifluoroactetate salt

(112) ##STR00335##

(113) To a solution of tert-butyl N-[2-oxo-2-[[4-(2-pyridyl)thiazol-2-yl]amino]ethyl]carbamate (250 mg, 665.39 μmol) in DCM (2 mL) was added TFA (1.10 mL). The mixture was stirred at 25° C. for 1 h. The reaction mixture was evaporated to dryness. To the residue was added MTBE/PE=(2/1, 30 mL) and stirred for 10 min then filtered. The solid was dried in vacuum to give intermediate i-9a (TFA salt) as a yellow solid.

(114) LCMS (ESI) m/z: [M+H].sup.+=234.9.

Step 2: Preparation of 3-(dimethylsulfamoyl)-N-[2-oxo-2-[[4-(2-pyridyl)thiazol-2-yl]amino]ethyl]benzamide (Compound 32)

(115) ##STR00336##

(116) To a mixture of 3-(dimethylsulfamoyl)benzoic acid (98.73 mg, 430.66 μmol) in DCM (2 mL) was added HATU (180.13 mg, 473.73 μmol) and DIPEA (278.30 mg, 2.15 mmol) at 25° C. The mixture was stirred at 25° C. for 10 min, then intermediate 9a (150 mg, 430.66 μmol) was added and stirred for 1 h. The reaction mixture was evaporated to dryness. To the residue was added MeOH (30 mL) and stirred for 10 min, then filtered and the filter cake was washed with MeOH (30 mL). The filter cake was evaporated to dryness to give compound 32 as an off-white solid.

(117) .sup.1HNMR (400 MHz, DMSO-d.sub.6) δ=12.49 (br s, 1H), 9.30-9.28 (m, 1H), 8.60 (d, J=4.0 Hz, 1H), 8.27-8.21 (m, 2H), 7.98-7.92 (m, 2H), 7.92-7.86 (m, 1H), 7.85-7.80 (m, 2H), 7.36-7.32 (m, 1H), 4.24 (d, J=5.6 Hz, 2H), 2.66 (s, 6H) ppm;

(118) LCMS (ESI) m/z: [M+H].sup.+=446.2.

Example 10. Preparation of Compound N-[(1S)-1-[(4-phenylthiazol-2-yl)carbamoyl]propyl]benzamide (Compound 45)

(119) ##STR00337##

Step 1: Preparation of tert-butyl N-[(1S)-1-[(4-phenylthiazol-2-yl)carbamoyl]propyl]carbamate (Intermediate i-10a)

(120) ##STR00338##

(121) To a solution of (2S)-2-(tert-butoxycarbonylamino)butanoic acid (200.0 mg, 984.08 μmol) in DCM (5.0 mL) were added EEDQ (331.85 mg, 1.34 mmol) and 4-phenylthiazol-2-amine (157.67 mg, 894.62 μmol) in one portion at 20° C. under N.sub.2. The mixture was stirred at 20° C. for 8 hours. The reaction mixture was diluted with EtOAc (50.0 mL), and washed with citric acid (10%) (50.0 mL*3). The organic phase was washed with saturated brine (20.0 mL*3) and dried over Na.sub.2SO.sub.4, filtered and concentrated under reduced pressure to give a residue. The residue was purified by column chromatography (SiO.sub.2, Petroleum ether/Ethyl acetate=20/1) to give intermediate i-10a as a white solid.

(122) .sup.1H NMR (400 MHz, CDCl.sub.3) δ 9.90 (br s, 1H), 7.94-7.77 (m, 2H), 7.50-7.38 (m, 2H), 7.37-7.31 (m, 1H), 7.15 (s, 1H), 5.10 (br s, 1H), 4.36 (br s, 1H), 1.50 (s, 9H), 1.40-1.22 (m, 2H), 1.07-0.97 (m, 3H) ppm.

(123) LCMS (ESI) m/z: [M+H].sup.+=362.2.

Step 2: Preparation of (2S)-2-amino-N-(4-phenylthiazol-2-yl)butanamide (Intermediate i-10b) trifluoroactetate salt

(124) ##STR00339##

(125) To a solution of intermediate i-10a (300.00 mg, 829.97 μmol) in DCM (10.0 mL) was added TFA (1.08 mL) in one portion at 20° C. under N.sub.2. The mixture was stirred at 20° C. for 2 hours. The reaction mixture was concentrated under reduced pressure to give intermediate i-10b (TFA salt) as a white solid, which was used in the next step without further purification.

(126) LCMS (ESI) m/z: [M+H].sup.+=262.2.

Step 3: Preparation of N-[(1S)-1-[(4-phenylthiazol-2-yl)carbamoyl]propyl]benzamide (Compound 45)

(127) ##STR00340##

(128) To a mixture of HATU (73.95 mg, 194.48 μmol) and benzoic acid (17.42 mg, 142.62 μmol) in DCM (10.0 mL) was added DIPEA (67.03 mg, 518.62 μmol) in one portion at 20° C. under N.sub.2. The mixture was stirred at 20° C. for 10 min. Then intermediate i-10b (50.0 mg, 191.32 μmol) was added and the mixture was stirred at 20° C. for 2 hours. The reaction mixture was concentrated under reduced pressure to give a residue. The residue was purified by prep-HPLC (TFA condition; column: Boston pH-lex 150*25 10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 47%-77%, 10 min) and lyophilized to give compound 45 as a white solid.

(129) .sup.1H NMR (400 MHz, CDCl.sub.3) δ=8.00 (d, J=7.2 Hz, 2H), 7.84 (d, J=7.6 Hz, 2H), 7.64-7.54 (m, 1H), 7.53-7.46 (m, 2H), 7.46-7.40 (m, 2H), 7.40-7.33 (m, 1H), 7.17 (s, 1H), 7.00 (br d, J=8.0 Hz, 1H), 5.18-5.12 (m, 1H), 2.22-2.17 (m, 1H), 2.05-1.97 (m, 1H), 1.14-1.11 (m, 3H) ppm.

(130) LCMS (ESI) m/z: [M+H].sup.+=366.0.

(131) Chiral HPLC: Amycoat-MeOH(DEA)-40-3 mL-35T, 1.417 min.

Example 11. Preparation of N-[(1S)-1-(hydroxymethyl)-2-oxo-2-[(4-phenylthiazol-2-yl)amino]ethyl]benzamide (Compound 47)

(132) ##STR00341##

Step 1: Preparation of tert-butyl N-[(1S)-1-(hydroxymethyl)-2-oxo-2-[(4-phenylthiazol-2-yl)amino]ethyl]carbamate (Intermediate i-11a)

(133) ##STR00342##

(134) To a mixture of (2S)-2-(tert-butoxycarbonylamino)-3-hydroxy-propanoic acid (200.0 mg, 974.62 μmol) in DCM (5.0 mL) was added EEDQ (328.66 mg, 1.33 mmol) and 4-phenylthiazol-2-amine (156.15 mg, 886.02 μmol) in one portion at 20° C. under N.sub.2. The mixture was stirred at 20° C. for 3 hours. The reaction mixture was diluted with EtOAc (50.0 mL), and washed with citric acid (10%) (50.0 mL*3). The organic phase was washed with brine (20.0 mL*3) and dried over Na.sub.2SO.sub.4, filtered and concentrated under reduced pressure to give a residue. The residue was purified by column chromatography (SiO.sub.2, Petroleum ether/Ethyl acetate=5/1 to 2:1) to give intermediate i-11a as colorless oil.

(135) LCMS (ESI) m/z: [M+H].sup.+=364.2.

Step 2: Preparation of ((2S)-2-amino-3-hydroxy-N-(4-phenylthiazol-2-yl)propanamide (Intermediate i-11b) trifluoroactetate salt

(136) ##STR00343##

(137) To a mixture of intermediate i-11a (130.00 mg, 182.07 μmol) in DCM (10.0 mL) was added trifluoroacetic acid (1.0 mL) in one portion at 20° C. under N.sub.2. The mixture was stirred at 20° C. for 2 hours. The reaction mixture was concentrated under reduced pressure to give intermediate i-11b (TFA salt) as a white solid and which was used in the next step without further purification.

(138) LCMS (ESI) m/z: [M+H].sup.+=264.2.

Step 3: Preparation of N-[(1S)-1-(hydroxymethyl)-2-oxo-2-[(4-phenylthiazol-2-yl)amino]ethyl]benzamide (Compound 47)

(139) ##STR00344##

(140) To a mixture of HATU (47.91 mg, 126.00 μmol) and benzoic acid (11.29 mg, 92.48 μmol) in DCM (2.0 mL) was added DIPEA (43.43 mg, 336.04 μmol) in one portion at 20° C. under N.sub.2, and the mixture was stirred at 20° C. for 10 min. Then intermediate i-11b (50.0 mg, 132.51 μmol) was added and the mixture was stirred at 20° C. for 2 hours. The reaction mixture was concentrated under reduced pressure to give a residue. The residue was purified by prep-HPLC (TFA condition; column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 35%-65%, 9 min) and lyophilized to give the compound 47 as a white solid.

(141) .sup.1H NMR (400 MHz, CDCl.sub.3) δ=7.93 (d, J=8.0 Hz, 2H), 7.84 (d, J=8.0 Hz, 2H), 7.63-7.57 (m, 1H), 7.52-7.37 (m, 5H), 7.18 (s, 1H), 5.02-4.99 (m, 1H), 4.47-4.43 (m, 1H), 3.96-3.92 (m, 1H) ppm.

(142) LCMS (ESI) m/z: [M+H].sup.+=368.0.

(143) Chiral HPLC: Amycoat-MeOH(DEA)-40-7 min-3 mL, 2.042 min.

Example 12. Preparation of N-[(1S)-2-methyl-1-[(4-phenylthiazol-2-yl)carbamoyl]propyl]benzamide (Compound 50)

(144) ##STR00345##

Step 1: Preparation of tert-butyl N-[(1S)-2-methyl-1-[(4-phenylthiazol-2-yl)carbamoyl]propyl]carbamate (Intermediate i-12a)

(145) ##STR00346##

(146) To a solution of (2S)-2-(tert-butoxycarbonylamino)-3-methyl-butanoic acid (200.0 mg, 920.55 μmol) in DCM (5.0 mL) were added EEDQ (310.42 mg, 1.26 mmol) and 4-phenylthiazol-2-amine (147.49 mg, 836.86 μmol) in one portion at 20° C. under N.sub.2. The mixture was stirred at 20° C. for 8 hours. The reaction mixture was diluted with EtOAc (50.0 mL), and washed with citric acid (10%) (50.0 mL*3). The combined organic layers were washed with saturated brine (20.0 mL*3), dried over Na.sub.2SO.sub.4, filtered and concentrated under reduced pressure to give a residue. The residue was purified by column chromatography (SiO.sub.2, Petroleum ether/Ethyl acetate=20/1) to give intermediate i-12a as a colorless oil.

(147) LCMS (ESI) m/z: [M+H].sup.+=376.2.

Step 2: Preparation of (2S)-2-amino-3-methyl-N-(4-phenylthiazol-2-yl)butanamide (Intermediate i-12b) trifluoroacetate salt

(148) ##STR00347##

(149) To a mixture of Intermediate i-12a (300.00 mg, 629.59 μmol) in DCM (10.0 mL) was added trifluoroacetic acid (1.0 mL) in one portion at 20° C. under N.sub.2. The mixture was stirred at 20° C. for 2 hours. The reaction mixture was concentrated under reduced pressure to give intermediate i-12b (TFA salt) as a white solid which was used into next step without purification.

(150) LCMS (ESI) m/z: [M+H].sup.+=276.2.

Step 3: Preparation of N-[(1S)-2-methyl-1-[(4-phenylthiazol-2-yl)carbamoyl]propyl]benzamide (Compound 50)

(151) ##STR00348##

(152) To a mixture of HATU (61.81 mg, 162.56 μmol) and benzoic acid (14.56 mg, 119.23 μmol) in DCM (2.0 mL) was added DIPEA (56.03 mg, 433.53 μmol) in one portion at 20° C. under N.sub.2. The mixture was stirred at 20° C. for 10 min. Then intermediate i-12b (50.0 mg, 128.41 μmol) was added and the mixture was stirred at 20° C. for 2 hours. The reaction mixture was concentrated under reduced pressure to give a residue. The residue was purified by prep-HPLC (TFA condition; column: Boston pH-lex 150*25 10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 50%-80%, 10 min) and lyophilized to give compound 50 as a white solid.

(153) .sup.1H NMR (400 MHz, CDCl.sub.3) δ 10.52 (br s, 1H), 7.99 (d, J=8.0 Hz, 2H), 7.81 (d, J=8.0 Hz, 2H), 7.61-7.54 (m, 1H), 7.52-7.45 (m, 2H), 7.43-7.36 (m, 2H), 7.36-7.29 (m, 1H), 7.16 (s, 1H), 6.91 (d, J=8.0 Hz, 1H), 5.24-5.09 (m, 1H), 2.46-2.38 (m, 1H), 1.16-1.13 (m, 6H).

(154) LCMS (ESI) m/z: [M+H].sup.+=380.0.

(155) Chiral HPLC: Cellucoat-MeOH(DEA)-5-40-3 mL-35, 1.981 min.

Example 13. Preparation of Compound N-[(1S)-1-[(4-phenylthiazol-2-yl)carbamoyl]butyl]benzamide (Compound 51)

(156) ##STR00349##

Step 1: Preparation of tert-butyl N-[(1S)-1-[(4-phenylthiazol-2-yl)carbamoyl]butyl]carbamate (Intermediate i-13a)

(157) ##STR00350##

(158) To a mixture of (2S)-2-(tert-butoxycarbonylamino)pentanoic acid (200.0 mg, 920.55 μmol) in DCM (5.0 mL) was added EEDQ (310.42 mg, 1.26 mmol) and 4-phenylthiazol-2-amine (147.49 mg, 836.86 μmol) in one portion at 20° C. under N.sub.2. The mixture was stirred at 20° C. for 8 hours. The reaction mixture was diluted with EtOAC (50.0 mL), and washed with citric acid (10%) (50.0 mL*3). The combined organic layers were washed with saturated brine (20.0 mL*3), dried over Na.sub.2SO.sub.4, filtered and concentrated under reduced pressure to give a residue. The residue was purified by column chromatography (SiO.sub.2, Petroleum ether/Ethyl acetate=20/1) to give intermediate i-13a as colorless oil.

(159) LCMS (ESI) m/z: [M+H].sup.+=376.3.

Step 2: Preparation of (2S)-2-amino-3-methyl-N-(4-phenylthiazol-2-yl)butanamide (Intermediate i-13b) trifluoroacetate salt

(160) ##STR00351##

(161) To a mixture of Intermediate i-13a (300.00 mg, 661.55 μmol) in DCM (10.0 mL) was added trifluoroacetic acid (937.48 μL) in one portion at 20° C. under N.sub.2. The mixture was stirred at 20° C. for 2 hours. The reaction mixture was concentrated under reduced pressure to give intermediate i-13b (TFA salt) as a white solid, which was used into the next step without further purification.

(162) LCMS (ESI) m/z: [M+H].sup.+=276.2.

Step 3: Preparation of N-[(1S)-1-[(4-phenylthiazol-2-yl)carbamoyl]butyl]benzamide (Compound 40)

(163) ##STR00352##

(164) To a mixture of HATU (51.26 mg, 134.83 μmol) and benzoic acid (15.09 mg, 123.59 μmol) in DCM (2.0 mL) was added DIPEA (58.08 mg, 449.42 μmol) in one portion at 20° C. under N.sub.2. The mixture was stirred at 20° C. for 10 min. Then intermediate i-13b (50.0 mg, 181.57 μmol, TFA salt) was added and the mixture was stirred at 20° C. for 2 hours. The reaction mixture was concentrated under reduced pressure to give a residue. The residue was purified by prep-HPLC (TFA condition; column: Boston pH-lex 150*25 10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 50%-80%, 10 min) and lyophilized to give compound 51 as a white solid.

(165) .sup.1H NMR (400 MHz, CDCl.sub.3) δ 7.95-7.93 (m, 2H), 7.78-7.76 (m, 2H), 7.58-7.51 (m, 1H), 7.48-7.41 (m, 5H), 7.12 (s, 1H), 7.08-6.99 (m, 1H), 5.04-4.99 (m, 1H), 2.09-2.03 (m, 1H), 1.95-1.92 (m, 1H), 1.56-1.50 (m, 2H), 1.04-1.00 (m, 3H) ppm.

(166) LCMS (ESI) m/z: [M+H].sup.+=380.0.

(167) Chiral HPLC: Cellucoat-MeOH(DEA)-5-40-3 mL-3, 2.141 min.

Example 14. Preparation of N-[(1S)-3-methyl-1-[(4-phenylthiazol-2-yl)carbamoyl]butyl]benzamide (Compound 48)

(168) ##STR00353##

Step 1: Preparation of tert-butyl N-[(1S)-3-methyl-1-[(4-phenylthiazol-2-yl)carbamoyl]butyl]carbamate (Intermediate i-14a)

(169) ##STR00354##

(170) To a mixture of (2S)-2-(tert-butoxycarbonylamino)-4-methyl-pentanoic acid; hydrate (200.00 mg, 802.24 μmol) in DCM (5.0 mL) was added EEDQ (270.53 mg, 1.09 mmol) and 4-phenylthiazol-2-amine (128.53 mg, 729.31 μmol) in one portion at 20° C. under N.sub.2. The mixture was stirred at 20° C. for 3 hours. The reaction mixture was diluted with EA (50.0 mL), and washed with citric acid (10%) (50.0 mL*3). The organic phase was washed with saturated brine (20.0 mL*3) and dried over Na.sub.2SO.sub.4, filtered and concentrated under reduced pressure to give a residue. The residue was purified by column chromatography (SiO.sub.2, Petroleum ether/Ethyl acetate=20/1) to give intermediate i-14a as colorless oil.

(171) LCMS (ESI) m/z: [M+H].sup.+=390.3.

Step 2: Preparation of (2S)-2-amino-3-methyl-N-(4-phenylthiazol-2-yl)butanamide (Intermediate i-14b) trifluoroacetate salt

(172) ##STR00355##

(173) To a solution of intermediate i-14a (170.00 mg, 392.80 μmol) in DCM (10.0 mL) was added trifluoroacetic acid (742.95 μL) in one portion at 20° C. under N.sub.2. The mixture was stirred at 20° C. for 2 hours. The reaction mixture was concentrated under reduced pressure to give intermediate i-14b (TFA salt) as a white solid, which was used into the next step without further purification. LCMS (ESI) m/z: [M+H].sup.+=290.2.

Step 3: Preparation of N-[(1S)-3-methyl-1-[(4-phenylthiazol-2-yl)carbamoyl]butyl]benzamide (Compound 48)

(174) ##STR00356##

(175) To a mixture of HATU (69.13 mg, 181.81 μmol) and benzoic acid (16.28 mg, 133.31 μmol) in DCM (2.0 mL) was added DIPEA (62.66 mg, 484.82 μmol) in one portion at 20° C. under N.sub.2. The mixture was stirred at 20° C. for 10 min. Then intermediate i-14b (50.0 mg, 172.77 μmol TFA salt) was added and the mixture was stirred at 20° C. for 2 hours. The reaction mixture was concentrated under reduced pressure to give a residue. The residue was purified by prep-HPLC (TFA condition; column: Boston pH-lex 150*25 10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 53%-83%, 10 min) to give compound 48 as a white solid.

(176) .sup.1H NMR (400 MHz, CDCl.sub.3) δ 10.19 (br s, 1H), 7.92-7.87 (m, 2H), 7.86-7.81 (m, 2H), 7.61-7.55 (m, 1H), 7.52-7.46 (m, 2H), 7.45-7.38 (m, 2H), 7.37-7.30 (m, 1H), 7.16 (s, 1H), 6.59 (br d, J=8.0 Hz, 1H), 5.10- 5.00 (m, 1H), 2.02-1.98 (m, 1H), 1.84-1.79 (m, 2H), 1.05-1.02 (m, 6H) ppm.

(177) LCMS (ESI) m/z: [M+H].sup.+=394.1.

(178) Chiral HPLC: Cellucoat-MeOH(DEA)-5-40-3 mL-3, 1.875 min.

Example 15. Preparation of (1S)-3-methyl-1-[(4-phenylthiazol-2-yl)carbamoyl]butyl]benzamide (Compound 41)

(179) ##STR00357##

Step 1: Preparation of tert-butyl N-[(1S)-1-[(4-phenylthiazol-2-yl)carbamoyl]pentyl]carbamate (Intermediate i-15a)

(180) ##STR00358##

(181) To a solution of (2S)-2-(tert-butoxycarbonylamino)hexanoic acid (200.0 mg, 864.72 μmol) in DCM (5.0 mL) were added EEDQ (291.60 mg, 1.18 mmol) and 4-phenylthiazol-2-amine (138.54 mg, 786.11 μmol) in one portion at 20° C. under N.sub.2. The mixture was stirred at 20° C. for 8 hours. The reaction mixture was diluted with EtOAc (50.0 mL) and washed with citric acid (10%) (50.0 mL*3). The organic phase was washed with saturated brine (20.0 mL*3) and dried over Na.sub.2SO.sub.4, filtered and concentrated under reduced pressure to give a residue. The residue was purified by column chromatography (SiO.sub.2, Petroleum ether/Ethyl acetate=20/1). Intermediate i-15a was obtained as a white solid.

(182) LCMS (ESI) m/z: [M+H].sup.+=390.3.

Step 2: Preparation of (2S)-2-amino-N-(4-phenylthiazol-2-yl)hexanamide (Intermediate i-15b)

(183) ##STR00359##

(184) To a mixture of Intermediate i-15a (230.00 mg, 590.48 μmol) in DCM (10.0 mL) was added trifluoroacetic acid (1.03 mL) in one portion at 20° C. under N.sub.2. The mixture was stirred at 20° C. for 2 hours. The reaction mixture was concentrated under reduced pressure to give intermediate i-15b (TFA salt) as a white solid, which was used in the next step without further purification.

(185) LCMS (ESI) m/z: [M+H].sup.+=290.2.

Step 3: Preparation of N-[(1S)-3-methyl-1-[(4-phenylthiazol-2-yl)carbamoyl]butyl]benzamide (Compound 41)

(186) ##STR00360##

(187) To a mixture of HATU (67.32 mg, 177.05 μmol) and benzoic acid (15.86 mg, 129.84 μmol) in DCM (5.0 mL) was added DIPEA (61.02 mg, 472.15 μmol) in one portion at 20° C. under N.sub.2. The mixture was stirred at 20° C. for 10 min. Then Intermediate i-15b (50.0 mg, 123.94 μmol) was added and the mixture was stirred at 20° C. for 2 hours. The reaction mixture was concentrated under reduced pressure to give a residue. The residue was purified by prep-HPLC (TFA condition; column: Boston pH-lex 150*25 10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 53%-83%, 10 min) and lyophilized to give compound 41 as a white solid.

(188) .sup.1H NMR (400 MHz, CDCl.sub.3) δ 7.98-7.96 (m, 2H), 7.83-7.81 (m, 2H), 7.62-7.55 (m, 1H), 7.53-7.42 (m, 4H), 7.41-7.35 (m, 1H), 7.15 (s, 1H), 7.04-6.95 (m, 1H), 5.13-5.08 (m, 1H), 2.15-2.11 (m, 1H), 1.98-1.94 (m, 1H), 1.51-1.41 (m, 4H), 0.96-0.92 (m, 3H) ppm.

(189) LCMS (ESI) m/z: [M+H].sup.+=394.0.

(190) Chiral HPLC: Amycoat-MeOH(DEA)-40-3 mL-35T, 1.431 min.

Example 16. Preparation of Compound N-[(1S)-3-phenyl-1-[(4-phenylthiazol-2-)carbamoyl]propyl]benzamide (Compound 49)

(191) ##STR00361##

Step 1: Preparation of tert-butyl N-[(1S)-3-phenyl-1-[(4-phenylthiazol-2-yl)carbamoyl]propyl]carbamate (Intermediate i-16a)

(192) ##STR00362##

(193) To a mixture of 4-phenylthiazol-2-amine (114.71 mg, 650.91 μmol) in DCM (5 mL) was added EEDQ (241.44 mg, 976.36 μmol) and (2S)-2-(tert-butoxycarbonylamino)-4-phenyl-butanoic acid (200.0 mg, 716.00 μmol) in one portion at 20° C. under N.sub.2. The mixture was stirred at 20° C. for 8 hours. The reaction mixture was diluted with EtOAc (50.0 mL), and washed with citric acid (10%) (50.0 mL*3). The organic phase was washed with saturated brine (20.0 mL*3) and dried over Na.sub.2SO.sub.4, filtered and concentrated under reduced pressure to give a residue. The residue was purified by column chromatography (SiO.sub.2, Petroleum ether/Ethyl acetate=10/1). Intermediate i-16a was obtained as a white solid.

(194) LCMS (ESI) m/z: [M+H].sup.+=438.3.

Step 2: Preparation of (2S)-2-amino-4-phenyl-N-(4-phenylthiazol-2-yl)butanamide (Intermediate i-16b)

(195) ##STR00363##

(196) To a mixture of Intermediate i-16a (270.00 mg, 569.67 μmol) in DCM (10.0 mL) was added trifluoroacetic acid (923.19 μL) in one portion at 20° C. under N.sub.2. The mixture was stirred at 20° C. for 1 hour. The reaction mixture was concentrated under reduced pressure to give intermediate i-16b (TFA salt) as yellow oil, which was used in the next step without further purification.

(197) LCMS (ESI) m/z: [M+H].sup.+=338.2.

Step 3: Preparation of N-[(1S)-3-phenyl-1-[(4-phenylthiazol-2-yl)carbamoyl]propyl]benzamide (Compound 49)

(198) ##STR00364##

(199) To a mixture of HATU (57.62 mg, 151.54 μmol) and benzoic acid (13.57 mg, 111.13 μmol) in DCM (2.0 mL) was added DIPEA (52.23 mg, 404.11 μmol) in one portion at 20° C. under N.sub.2. The mixture was stirred at 20° C. for 10 min. Then intermediate i-16b (50.0 mg, 110.75 μmol) was added and the mixture was stirred at 20° C. for 2 hours. The reaction mixture was concentrated under reduced pressure to give a residue. The residue was triturated in MeOH (10.0 mL), the solid was collected by filtration and dried in vacuum to give compound 49 as a white solid.

(200) .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ=12.54 (s, 1H), 8.85 (d, J=7.2 Hz, 1H), 8.02-7.95 (m, 4H), 7.69 (s, 1H), 7.67-7.61 (m, 1H), 7.61-7.54 (m, 2H), 7.53-7.46 (m, 2H), 7.42-7.29 (m, 5H), 7.28-7.22 (m, 1H), 4.80- 4.74 (m, 1H), 2.87-2.75 (m, 1H), 2.55-2.45 (m, 1H), 2.23-2.19 (m, 2H).

(201) LCMS (ESI) m/z: [M+H].sup.+=442.1.

(202) Chiral HPLC: Amycoat-MeOH(DEA)-40-7 min-3 mL, 3.118 min.

Example 17. Preparation of Compound 3-(dimethylsulfamoyl)-N-[2-[[4-(o-tolyl)thiazol-2-yl]amino]-2-oxo-ethyl]benzamide (Compound 16)

(203) ##STR00365##

Step 1: Preparation of tert-butyl (2-oxo-2-((4-(o-tolyl)thiazol-2-yl)amino)ethyl)carbamate (Intermediate i-17a)

(204) ##STR00366##

(205) To a solution of 2-(tert-butoxycarbonylamino)acetic acid (184.15 mg, 1.05 mmol) in DCM (2.0 mL) was added DIEA (407.57 mg, 3.15 mmol) and HATU (439.65 mg, 1.16 mmol). After 0.5 hr, 4-(o-tolyl)thiazol-2-amine (200.0 mg, 1.05 mmol) was added and the mixture was stirred at 20° C. for 1.5 hr. The reaction mixture was poured into water (10.0 mL) and extracted with EtOAc (10.0 mL*3). The combined organic layers were washed with brine (10.0 mL), dried over Na.sub.2SO.sub.4, filtered and concentrated. The crude product was triturated with MTBE (10.0 mL). The solid was collected by filtration and dried in vacuo to give intermediate i-17a as white solid.

(206) .sup.1H NMR (400 MHz, CDCl.sub.3) δ 10.53-10.01 (m, 1H), 7.51 (s, 1H), 7.32-7.30 (m, 6H), 6.94 (s, 1H), 5.01 (br s, 1H), 3.87 (br s, 2H), 2.41 (s, 3H), 1.46 (s, 9H) ppm

(207) LCMS (ESI) m/z: [M+H].sup.+=348.1

Step 2: Preparation of 2-amino-N-(4-(o-tolyl)thiazol-2-yl)acetamide (Intermediate i-17b)

(208) ##STR00367##

(209) To a solution of intermediate i-17a (210 mg, 604.44 μmol) in DCM (2.1 mL) was added TFA (0.3 mL) at 20° C. The mixture was stirred at 20° C. for 16 hr. The reaction mixture was poured into sat.NaHCO.sub.3 (10.0 mL) and extracted with EtOAc (20.0 mL*3). The combined organic extracts were washed with brine (20.0 mL), dried over Na.sub.2SO.sub.4, filtered and concentrated. The residue was purified by column chromatography (SiO.sub.2, PE:EA=10:1 to 8:1 to 5:1) to give intermediate i-17b as brown oil.

(210) LCMS (ESI) m/z: [M+H].sup.+=248.1

Step 3: Preparation of -(dimethylsulfamoyl)-N-[2-[[4-(o-tolyl)thiazol-2-yl]amino]-2-oxo-ethyl]benzamide (Compound 16)

(211) ##STR00368##

(212) To a solution of 3-(dimethylsulfamoyl)benzoic acid (111.24 mg, 485.21 μmol) in DCM (2.0 mL) was added HATU (202.94 mg, 533.73 μmol) and DIEA (188.13 mg, 1.46 mmol). The mixture was stirred at 20° C. for 0.5 hr. Intermediate D (120.0 mg, 485.21 μmol) was added and the mixture was stirred at 20° C. for 1.5 hr. The reaction mixture was poured into water (10.0 mL) and extracted with EtOAc (10.0 mL*3). The combined organic extract were washed with brine (10.0 mL), dried over Na.sub.2SO.sub.4, filtered and concentrated. The crude product was triturated with MeOH (10.0 mL). The solid was collected by filtration and dried in vacuo to give compound 16 as white solid.

(213) .sup.1H NMR (400 MHz, DMSO-d6) δ 12.40 (s, 1H), 9.31-9.28 (m, 1H), 8.26-8.22 (m, 2H), 7.95-7.93 (m, 1H), 7.82-7.80 (m, 1H), 7.59-7.57 (m, 1H), 7.27-7.25 (m, 4H), 4.22 (d, J=5.6 Hz, 2H), 2.65 (s, 6H), 2.43 (s, 3H) ppm

(214) LCMS (ESI) m/z: [M+H].sup.+=459.0

Example 18. Preparation of Compound 3-(dimethylsulfamoyl)-N-[2-[[4-(m-tolyl)thiazol-2-yl]amino]-2-oxo-ethyl]benzamide (Compound 1)

(215) ##STR00369##

Step 1: Preparation of 4-(m-tolyl)thiazol-2-amine (Intermediate i-18a)

(216) ##STR00370##

(217) To a mixture of 1-(m-tolyl)ethanone (100.0 mg, 745.30 μmol), thiourea (85.10 mg, 1.12 mmol) and 12 (189.16 mg, 745.30 μmol) in EtOH (1.0 mL) was added NaF (10 mg, 238.16 μmol). The mixture was stirred at 20° C. for 3 hr. The reaction mixture was poured into water (8.0 mL) and extracted with EtOAc (10.0 mL*3). The combined organic layers were washed with brine (10.0 mL), dried over Na.sub.2SO.sub.4, filtered and concentrated in vacuum to give intermediate i-18a as green oil which was used directly in the next step.

(218) LCMS (ESI) m/z: [M+H].sup.+=191.1

Step 2: Preparation of tert-butyl (2-oxo-2-((4-(m-tolyl)thiazol-2-yl)amino)ethyl)carbamate (Intermediate i-18b)

(219) ##STR00371##

(220) To a solution of 2-(tert-butoxycarbonylamino)acetic acid (138.11 mg, 788.38 μmol) in DCM (2.0 mL) were added DIEA (305.68 mg, 2.37 mmol) and HATU (329.74 mg, 867.22 μmol) at 20° C. The mixture was stirred at 20° C. for 0.5 hr. Intermediate i-18a (150.0 mg, 788.38 μmol) was added and the mixture was stirred at 20° C. for 1.5 hr. The reaction mixture was poured into water (10.0 mL) and extracted with EtOAc (10.0 mL*3). The combined organic extracts were washed with brine (10.0 mL), dried over Na.sub.2SO.sub.4, filtered and concentrated to give intermediate i-18b as brown oil, which was used directly in the next step.

(221) LCMS (ESI) m/z: [M+H].sup.+=348.1

Step 3: Preparation of 2-amino-N-(4-(m-tolyl)thiazol-2-yl)acetamide (Intermediate i-18c)

(222) ##STR00372##

(223) To a solution of intermediate i-18b (75 mg, 215.87 μmol) in DCM (1.0 mL) was added TFA (0.25 mL) at 20° C. The mixture was stirred at 20° C. for 2 hr. The reaction mixture was poured into sat.Na.sub.2CO.sub.3 (10.0 mL) and extracted with EtOAc (10.0 mL*3). The combined organic extracts were washed with brine (10.0 mL), dried over Na.sub.2SO.sub.4, filtered and concentrated. The residue was purified by prep-HPLC (column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 15%-45%, 9 min) to give a fraction. Then the fraction was concentrated in vacuo, then poured into sat.Na.sub.2CO.sub.3 (15.0 mL) and extracted with EtOAc (20.0 mL*3). The combined organic extracts were washed with brine (10.0 mL), dried over Na.sub.2SO.sub.4, filtered and concentrated to give intermediate i-18c as yellow oil.

(224) LCMS (ESI) m/z: [M+H].sup.+=248.1

Step 4: Preparation of 3-(dimethylsulfamoyl)-N-[2-[[4-(m-tolyl)thiazol-2-yl]amino]-2-oxo-ethyl]benzamide (Compound 1)

(225) ##STR00373##

(226) To a solution of 3-(dimethylsulfamoyl)benzoic acid (46.35 mg, 202.17 μmol) in DCM (1.0 mL) were added HATU (84.56 mg, 222.39 μmol) and DIEA (78.39 mg, 606.51 μmol) at 20° C. The mixture was stirred at 20° C. for 0.5 hr. Then intermediate i-18c (50.0 mg, 202.17 μmol) was added and the mixture was stirred at 20° C. for 1.5 hr. The reaction mixture was poured into water (5.0 mL) and extracted with EtOAc (10.0 mL*3). The combined organic extracts were washed with brine (10.0 mL), dried over Na.sub.2SO.sub.4, filtered and concentrated. The residue was purified by prep-HPLC (column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 42%-72%, 9 min) and lyophilized to give compound 1 as white solid.

(227) 1H NMR (400 MHz, DMSO-d6) δ=12.45 (s, 1H), 9.30-9.27 (m, 1H), 8.26-8.22 (m, 2H), 7.94 (d, J=7.6 Hz, 1H), 7.82-7.77 (m, 1H), 7.73-7.65 (m, 2H), 7.60 (s, 1H), 7.32-7.30 (m, 1H), 7.14 (d, J=7.6 Hz, 1H), 4.23 (d, J=5.6 Hz, 2H), 2.65 (s, 6H), 2.35 (s, 3H) ppm LCMS (ESI) m/z: [M+H].sup.+=459.0

Example 19. Preparation of 3-(dimethylsulfamoyl)-N-[2-oxo-2-[[4-(p-tolyl)thiazol-2-yl]amino]ethyl]benzamide (Compound 31)

(228) ##STR00374##

Step 1: Preparation of tert-butyl N-[2-oxo-2-[[4-(p-tolyl)thiazol-2-yl]amino]ethyl]carbamate (Intermediate i-19a)

(229) ##STR00375##

(230) To a solution of 2-(tert-butoxycarbonylamino)acetic acid (184.15 mg, 1.05 mmol) in DCM (2.0 mL) was added DIEA (407.57 mg, 3.15 mmol) and HATU (439.65 mg, 1.16 mmol). After 0.5 hr, 4-(o-tolyl)thiazol-2-amine (200.0 mg, 1.05 mmol) was added the mixture and stirred at 20° C. for 1.5 hr. The reaction mixture was poured into water (10.0 mL) and extracted with EtOAc (10.0 mL*3). The combined organic extracts were washed with brine (10.0 mL), dried over Na.sub.2SO.sub.4, filtered and concentrated. The crude product was triturated with MTBE (10.0 mL). The solid was collected by filtration and dried in vacuo to give intermediate i-19a as a white solid.

(231) 1H NMR (400 MHz, CDCl.sub.3) δ 9.75 (br s, 1H), 7.71 (d, J=8.0 Hz, 2H), 7.23 (d, J=8.0 Hz, 2H), 7.11 (s, 1H), 5.22-5.03 (m, 1H), 4.20-3.94 (m, 2H), 2.39 (s, 3H), 1.50 (s, 9H).

(232) LCMS (ESI) m/z: [M+H].sup.+=348.1

Step 2: Preparation of 2-amino-N-[4-(p-tolyl)thiazol-2-yl]acetamide (i-19b)

(233) ##STR00376##

(234) To a solution of intermediate i-19a (210 mg, 604.44 μmol) in DCM (2.1 mL) was added TFA (0.3 mL) at 20° C. The mixture was stirred at 20° C. for 16 hr. The reaction mixture was poured into sat.Na.sub.2CO.sub.3 (10.0 mL) and extracted with EtOAc (20.0 mL*3). The combined organic extracts were washed with brine (20.0 mL), dried over Na.sub.2SO.sub.4, filtered and concentrated. The crude product was triturated with MTBE (10.0 mL). The solid was collected by filtration and dried in vacuum to give intermediate i-19b as a white solid.

(235) LCMS (ESI) m/z: [M+H].sup.+=248.1

Step 3: Preparation of 3-(dimethylsulfamoyl)-N-[2-oxo-2-[[4-(p-tolyl)thiazol-2-yl]amino]ethyl]benzamide (Compound 31)

(236) ##STR00377##

(237) To a solution of 3-(dimethylsulfamoyl)benzoic acid (139 mg, 606.32 μmol) in DCM (2.0 mL) were added DIEA (235.09 mg, 1.82 mmol) and HATU (253.59 mg, 666.95 μmol), the mixture was stirred at 20° C. for 0.5 hr. Then intermediate D (150.00 mg, 606.51 μmol) was added at 20° C. and the mixture was stirred at 20° C. for 1.5 hr. The reaction mixture was poured into water (10.0 mL) and extracted with EtOAc (10.0 mL*3). The combined organic extracts were washed with brine (10.0 mL), dried over Na.sub.2SO.sub.4, filtered and concentrated. The crude product was triturated with MTBE (10.0 mL). The solid was collected by filtration and dried in vacuo to give compound 31 as a white solid.

(238) 1H NMR (400 MHz, DMSO-d.sub.6) δ=12.45 (s, 1H), 9.30-9.27 (m, 1H), 8.26-8.22 (m, 2H), 7.94 (d, J=8.0 Hz, 1H), 7.82-7.77 (m, 3H), 7.56 (s, 1H), 7.24 (d, J=8.0 Hz, 2H), 4.23 (d, J=5.6 Hz, 2H), 2.65 (s, 6H), 2.32 (s, 3H) ppm

(239) LCMS (ESI) m/z: [M+H].sup.+=459.0

Example 20. Preparation of N-[2-[[4-(2,6-dimethylphenyl)thiazol-2-yl]amino]-2-oxo-ethyl]-3-(dimethylsulfamoyl)benzamide (Compound 29)

(240) ##STR00378##

(241) To a solution of N-[2-[(4-bromothiazol-2-yl)amino]-2-oxo-ethyl]-3-(dimethylsulfamoyl)benzamide (50 mg, 111.78 μmol) and (2,6-dimethylphenyl)boronic acid (50.29 mg, 335.33 μmol) in dioxane (2 mL) and H.sub.2O (0.1 mL) was added DIPEA (28.89 mg, 223.55 μmol), then Pd(t-Bu.sub.3P).sub.2 (28.56 mg, 55.89 μmol) was added. The reaction mixture was degassed three times and then stirred at 100° C. for 12 hr. The reaction solution was diluted with EtOAc (100 mL) and then washed with aq. NaHCO.sub.3 (100 mL) and brine (100 mL), the organic layer was dried over Na.sub.2SO.sub.4 and then concentrated in vacuum. The residue was purified through Prep-HPLC (combiflash, CH.sub.3CN/H.sub.2O: 5˜95%, NH.sub.3OH) to give compound 29 as a pink solid.

(242) .sup.1HNMR (400 MHz, MeOD) δ=8.34 (s, 1H), 8.23 (d, J=7.2 Hz, 1H), 8.01 (d, J=8.0 Hz, 1H), 7.80-7.76 (m, 1H), 7.20-7.16 (m, 1H), 7.11-7.09 (m, 2H), 6.91 (s, 1H), 4.36 (s, 2H), 2.76 (s, 6H), 2.12 (s, 6H) ppm.

(243) LCMS (ESI) m/z: [M+H].sup.+=473.3.

Example 21. Preparation of Compound N-[2-oxo-2-[(4-phenylthiazol-2-yl)amino]ethyl]-5,6-dihydro-4H-cyclopenta[b]thiophene-2-carboxamide (compound 79)

(244) ##STR00379##

(245) To a solution of 5,6-dihydro-4H-cyclopenta[b]thiophene-2-carboxylic acid (30.0 mg, 178.35 μmol), HATU (67.81 mg, 178.35 μmol) and DIPEA (83.82 mg, 648.53 μmol, 112.96 μL) in DCM (0.5 mL) was added 2-amino-N-(4-phenylthiazol-2-yl)acetamide (56.31 mg, 162.13 μmol, TFA salt). The mixture was stirred at 20° C. for 2 h. The reaction mixture was concentrated under reduced pressure to give a residue. The residue was triturated with MeOH (10.0 mL), the solid was collected by filtration and dried in vacuum to give compound 79 as a white solid

(246) 1H NMR (400 MHz, DMSO-d.sub.6) δ=12.41 (s, 1H), 8.79-8.76 (m, 1H), 7.91 (d, J=7.2 Hz, 2H), 7.64-7.58 (m, 2H), 7.46-7.42 (m, 2H), 7.37-7.30 (m, 1H), 4.15 (d, J=6.0 Hz, 2H), 2.90-2.87 (m, 2H), 2.78-2.69 (m, 2H), 2.41-2.37 (m, 2H) ppm.

(247) LCMS (ESI) m/z: [M+H].sup.+=384.0.

Example 22. Preparation of Compound N-[(1S)-4-methyl-1-[(4-phenylthiazol-2-yl)carbamoyl]pentyl]benzamide (Compound 46)

(248) ##STR00380##

Step 1: Preparation of tert-butyl N-[(1S)-4-methyl-1-[(4-phenylthiazol-2-yl)carbamoyl]pentyl]carbamate (Intermediate i-22a)

(249) ##STR00381##

(250) To a solution of (2S)-2-(tert-butoxycarbonylamino)-5-methyl-hexanoic acid (153.11 mg, 624.15 μmol) and EEDQ (210.47 mg, 851.12 μmol) in DCM (2.0 mL) was added 4-phenylthiazol-2-amine (100.0 mg, 567.41 μmol), the mixture was stirred at 20° C. for 12 hr. The reaction mixture was concentrated under reduced pressure to give a residue. The residue was purified by column chromatography (SiO.sub.2, Petroleum ether/Ethyl acetate=10:1 to 2:1) to give intermediate i-22a as a white solid.

(251) 1H NMR (400 MHz, DMSO-d.sub.6) δ=12.33 (s, 1H), 7.91 (d, J=7.2 Hz, 2H), 7.63 (s, 1H), 7.49-7.40 (m, 2H), 7.37-7.28 (m, 1H), 7.20 (d, J=7.6 Hz, 1H), 4.31-4.11 (m, 1H), 2.50-2.06 (m, 5H), 1.73-1.56 (m, 2H), 1.55-1.45 (m, 1H), 1.39 (s, 7H), 1.27 (s, 2H), 0.86-0.84 (m, 6H) ppm.

(252) LCMS (ESI) m/z: [M+H].sup.+=404.2.

Step 2: Preparation of (2S)-2-amino-5-methyl-N-(4-phenylthiazol-2-yl)hexanamide (Intermediate i-22b) trifluoroacetate salt

(253) ##STR00382##

(254) To a solution of intermediate i-22a (150.0 mg, 371.71 μmol) in DCM (1.0 mL) was added TFA (0.1 mL, 1.35 mmol), the mixture was stirred at 20° C. for 12 hr. The reaction mixture was concentrated under reduced pressure to give intermediate i-22b (TFA salt) as a white solid, which was used in the next step directly.

(255) LCMS (ESI) m/z: [M+H].sup.+=304.2.

Step 3: Preparation of N-[(1S)-4-methyl-1-[(4-phenylthiazol-2-yl)carbamoyl]pentyl]benzamide (Compound 46)

(256) ##STR00383##

(257) To a solution of benzoic acid (16.09 mg, 131.75 μmol, 20.11 μL), HATU (50.10 mg, 131.75 μmol) and DIPEA (61.92 mg, 479.11 μmol) in DCM (1.0 mL) was added intermediate i-22b (50.0 mg, 119.78 μmol, TFA salt), the mixture was stirred at 30° C. for 2 hr. The reaction mixture was concentrated under reduced pressure to give a residue. The residue was purified by prep-HPLC (column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 58%-88%, 9 min) and lyophilized to give compound 46 as a white solid.

(258) 1H NMR (400 MHz, DMSO-d6) δ 12.46 (s, 1H), 8.68 (d, J=7.2 Hz, 1H), 7.93-7.90 (m, 4H), 7.63 (s, 1H), 7.60-7.54 (m, 1H), 7.53-7.47 (m, 2H), 7.44-7.42 (m, 2H), 7.37-7.28 (m, 1H), 4.67-4.61 (m, 1H), 1.87- 1.83 (m, 2H), 1.59-1.56 (m, 1H), 1.48-1.32 (m, 1H), 1.31-1.15 (m, 1H), 0.90-0.88 (m, 6H) ppm.

(259) LCMS (ESI) m/z: [M+H].sup.+=408.2

(260) Chiral HPLC: Cellucoat-MeOH(DEA)-5-40-3 mL-35T.Icm, 1.982 min.

Example 23. Preparation of Compound 3-(dimethylsulfamoyl)-N-[(1S)-1-[(4-phenylthiazol-2-yl)carbamoyl]butyl]benzamide (Compound 17)

(261) ##STR00384##

(262) To a mixture of HATU (36.25 mg, 95.35 μmol) and 3-(dimethylsulfamoyl)benzoic acid (20.04 mg, 87.40 μmol) in DCM (2.0 mL) was added DIPEA (41.07 mg, 317.82 μmol) in one portion at 20° C. under N.sub.2. The mixture was stirred at 20° C. for 10 min. Then (2S)-2-amino-N-(4-phenylthiazol-2-yl)pentanamide (50.0 mg, 128.41 μmol, TFA salt) was added and the mixture was stirred at 20° C. for 2 hours. The reaction mixture was concentrated under reduced pressure to give a residue. The residue was purified by prep-HPLC (TFA condition; column: Boston pH-lex 150*25 10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 57%-76%, 8 min) and lyophilized to give compound 17 as a white solid.

(263) .sup.1H NMR (400 MHz, DMSO-d6) δ 12.52 (s, 1H), 9.05 (d, J=7.6 Hz, 1H), 8.29-8.27 (m, 2H), 7.93-7.90 (m, 3H), 7.81-7.79 (m, 1H), 7.64 (s, 1H), 7.54-7.39 (m, 2H), 7.38-7.27 (m, 1H), 4.76-4.70 (m, 1H), 2.68- 2.65 (m, 6H), 1.86-1.83 (m, 2H), 1.58-1.31 (m, 2H), 0.96-0.92 (m, 3H) ppm.

(264) LCMS (ESI) m/z: [M+H].sup.+=487.1.

(265) Chiral HPLC: Amycoat-MeOH(DEA)-40-7 min-3 mL, 2.022 min.

Example 24. Preparation of Compound N-[(1S)-1-methyl-2-oxo-2-[(4-phenylthiazol-2-yl)amino]ethyl]benzamide (Compound 44)

(266) ##STR00385##

Step 1: Preparation of tert-butyl N-[(1S)-1-methyl-2-oxo-2-[(4-phenylthiazol-2-yl)amino]ethyl]carbamate (Intermediate i-24a)

(267) ##STR00386##

(268) To a solution of (2S)-2-(tert-butoxycarbonylamino)propanoic acid (2.0 g, 10.57 mmol) in DCM (20.0 mL) were added EEDQ (3.56 g, 14.41 mmol) and 4-phenylthiazol-2-amine (1.69 g, 9.61 mmol) in one portion at 20° C. under N.sub.2. The mixture was stirred at 20° C. for 8 hours. The reaction mixture was diluted with EtOAc (50.0 mL), and washed with citric acid (10%) (50.0 mL*3). The organic phase was washed with brine (50.0 mL*3) and dried over Na.sub.2SO.sub.4, filtered and concentrated under reduced pressure to give a residue. The residue was purified by column chromatography (SiO.sub.2, Petroleum ether/Ethyl acetate=10/1 to 3/1) to give intermediate i-24a as a white solid.

(269) .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ=12.30 (s, 1H), 7.90 (d, J=8.0 Hz, 2H), 7.63 (s, 1H), 7.45-7.41 (m, 2H), 7.36-7.30 (m, 1H), 7.26 (d, J=8.0 Hz, 1H), 4.31-4.24 (m, 1H), 1.38-1.28 (m, 12H) ppm.

(270) LCMS (ESI) m/z: [M+H].sup.+=348.2.

Step 2: Preparation of (2S)-2-amino-N-(4-phenylthiazol-2-yl)propanamide (Intermediate i-24b) trifluoroacetate salt

(271) ##STR00387##

(272) To a solution of intermediate i-24a (200.0 mg, 575.65 μmol) in DCM (10.0 mL) was added trifluoroacetic acid (1.0 mL) in one portion at 20° C. under N.sub.2. The mixture was stirred at 20° C. for 2 hours. The reaction mixture was concentrated under reduced pressure to give intermediate i-24b (TFA salt) as a yellow oil, which was used in the next step without further purification.

(273) LCMS (ESI) m/z: [M+H].sup.+=248.2.

Step 3: Preparation of N-[(1S)-1-methyl-2-oxo-2-[(4-phenylthiazol-2-yl)amino]ethyl]benzamide (Compound 44)

(274) ##STR00388##

(275) To benzoic acid (37.18 mg, 304.42 μmol) in DCM (5.0 mL) was added DIPEA (143.07 mg, 1.11 mmol) and HATU (157.84 mg, 415.12 μmol) in one portion at 20° C. under N.sub.2, and the mixture was stirred at 20° C. for 10 min. Then intermediate i-24b (100.0 mg, 276.75 μmol) was added and the mixture was stirred at 20° C. for 2 hours. The reaction mixture was concentrated under reduced pressure to give a residue. The residue was purified by prep-HPLC (TFA condition; column: Boston pH-lex 150*25 10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 52%-72%, 8 min) and lyophilized to give compound 44 as a white solid.

(276) .sup.1H NMR (400 MHz, DMSO-d6) δ 12.38 (br s, 1H), 8.75 (d, J=8.0 Hz, 1H), 7.94-7.90 (m, 4H), 7.64 (s, 1H), 7.60-7.54 (m, 1H), 7.53-7.47 (m, 2H), 7.47-7.41 (m, 2H), 7.37-7.29 (m, 1H), 4.74-4.67 (m, 1H), 1.48 (d, J=8.0 Hz, 3H) ppm.

(277) LCMS (ESI) m/z: [M+H].sup.+=352.3.

(278) Chiral HPLC: Cellucoat-MeOH(DEA)-5-40-3 mL-35T.Icm, 2.262 min.

Example 25. Preparation of (S)-3-methyl-N-(4-(methylthio)-1-oxo-1-((4-phenylthiazol-2-yl)amino)butan-2-yl)benzamide (compound 76)

(279) ##STR00389##

(280) To a solution of 3-methylbenzoic acid (17.77 mg, 130.54 μmol) in DCM (1 mL) was added HATU (74.45 mg, 195.81 μmol) and DIPEA (50.61 mg, 391.61 μmol), then (2S)-2-amino-4-methylsulfanyl-N-(4-phenylthiazol-2-yl)butanamide (40.13 mg, 130.54 μmol) was added. The mixture was stirred at 25° C. for 2 hr. The reaction mixture was concentrated under reduced pressure. The residue was purified by prep-HPLC (TFA condition; column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 55%-85%, 9 min) and lyophilized to give compound 76 as a white solid. 1H NMR (400 MHz, METHANOL-d4) δ 7.90 (d, J=7.2 Hz, 2H), 7.73-7.70 (m, 2H), 7.42-7.34 (m, 5H), 7.30-7.27 (m, 1H), 4.93-4.89 (m, 1H), 2.71-2.60 (m, 2H), 2.42 (s, 3H), 2.30-2.17 (m, 2H), 2.14 (s, 3H) ppm.

(281) LCMS (ESI) m/z: [M+H].sup.+=426.0.

(282) SFC HPLC: OD-3_5CM_MEOH(DEA)_5_40_3ML_T3, 2.236 min.

Example 26. Preparation of Compound (S)-4-methyl-N-(4-(methylthio)-1-oxo-1-((4-phenylthiazol-2-yl)amino)butan-2-yl)benzamide (Compound 54)

(283) ##STR00390##

(284) To a solution of 4-methylbenzoic acid (17.77 mg, 130.54 μmol) in DCM (1 mL) was added HATU (74.45 mg, 195.81 μmol) and DIPEA (50.61 mg, 391.62 μmol), and then (2S)-2-amino-4-methylsulfanyl-N-(4-phenylthiazol-2-yl)butanamide (40.13 mg, 130.54 μmol) was added. The mixture was stirred at 25° C. for 2 hr. The reaction mixture was concentrated under reduced pressure. The residue was purified by prep-HPLC (TFA condition; column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 55%-85%, 9 min) to give compound 54 as a white solid.

(285) .sup.1HNMR (400 MHz, METHANOL-d4) δ 7.90 (d, J=7.2 Hz, 2H), 7.81 (d, J=8.0 Hz, 2H), 7.42-7.36 (m, 3H), 7.33-7.28 (m, 3H), 4.93-4.89 (m, 1H), 2.75-2.62 (m, 2H), 2.41 (s, 3H), 2.30-2.21 (m, 2H), 2.14 (s, 3H) ppm.

(286) LCMS (ESI) m/z: [M+H].sup.+=426.0.

(287) Chiral HPLC: OD-3_5CM_MEOH(DEA)_40_3ML_T35.M, 1.673 min.

Example 27. Preparation of (S)-3-(methylsulfonyl)-N-(4-(methylthio)-1-oxo-1-((4-phenylthiazol-2-yl)amino)butan-2-yl)benzamide (Compound 3)

(288) ##STR00391##

(289) To a solution of 3-methylsulfonylbenzoic acid (19.00 mg, 94.91 μmol) in DCM (2 mL) was added HATU (54.13 mg, 142.36 μmol) and DIPEA (49.07 mg, 379.64 μmol), then (2S)-2-amino-4-methylsulfanyl-N-(4-phenylthiazol-2-yl)butanamide (40 mg, 94.91 μmol) was added. The mixture was stirred at 10° C. for 2 hr. The reaction mixture was concentrated under reduced pressure. The residue was purified by prep-HPLC (TFA condition; column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 48%-78%, 9 min) to give compound 3 as a white solid.

(290) .sup.1HNMR (400 MHz, METHANOL-d4) δ 8.50 (s, 1H), 8.24 (d, J=8.0 Hz, 1H), 8.16 (d, J=8.0 Hz, 1H), 7.91-7.88 (m, 2H) 7.79-7.77 (m, 1H), 7.42-7.36 (m, 3H), 7.31-7.30 (m, 1H), 4.96-4.92 (m, 1H), 3.18 (s, 3H), 2.77-2.66 (m, 2H), 2.36-2.20 (m, 2H), 2.15 (s, 3H) ppm.

(291) LCMS (ESI) m/z: [M+H].sup.+=490.0.

Example 28. Preparation of 3-(tert-butyl)-N-(2-oxo-2-((4-phenylthiazol-2-yl)amino)ethyl)benzamide (Compound 65)

(292) ##STR00392##

(293) To a solution of 3-tert-butylbenzoic acid (25.66 mg, 143.96 μmol) in DCM (1 mL) was added HATU (82.11 mg, 215.94 μmol), DIPEA (93.03 mg, 719.81 μmol, 125.38 μL). The reaction mixture was stirred at 25° C. for 0.5 hrs, then 2-amino-N-(4-phenylthiazol-2-yl)acetamide (50 mg, 143.96 μmol) was added. The reaction mixture was stirred at 25° C. for 2 hr. The reaction mixture was diluted with MeCN (2 mL) and a white precipitate formed. The precipitate was filtered off and lyophilized to afford compound 65 as a white solid.

(294) LCMS (ESI) m/z: [M+H].sup.+=394.3.

(295) .sup.1H NMR (400 MHz, DMSO-d6) δ=12.44 (s, 1H), 8.96-8.94 (m, 1H), 7.94-7.90 (m, 3H), 7.73 (d, J=8.0 Hz, 1H), 7.64 (s, 1H), 7.60 (br d, J=8.4 Hz, 1H), 7.47-7.42 (m, 3H), 7.34-7.31 (m, 1H), 4.21 (d, J=5.6 Hz, 2H), 1.33 (s, 9H) ppm.

Example 29. Preparation of 3-(2-hydroxypropan-2-yl)-N-(2-oxo-2-((4-phenylthiazol-2-yl)amino)ethyl)benzamide (Compound 61)

(296) ##STR00393##

Step 1: Preparation of 3-(2-hydroxypropan-2-yl)benzoic acid (Intermediate i-29a)

(297) ##STR00394##

(298) To a solution of 3-bromobenzoic acid (500 mg, 2.49 mmol) in THF (10 mL) was added n-BuLi (2 mL, 4.97 mmol, 2.5 M) at −65° C. under N.sub.2. The reaction mixture was stirred at −65° C. for 10 min, then acetone (365 μL 4.97 mmol) was added at −65° C. The reaction mixture was warmed to 25° C. gradually and then stirred at room temperature for 50 min. The reaction mixture was poured into 20 mL saturated aqueous ammonium chloride. The mixture was adjusted to pH=4 with 2 M HCl. Then the mixture was extracted with EtOAc (30 mL×5). The combined organic extracts were washed with brine, dried over anhydrous sodium sulfate, filtered and concentrated to give residue. To the residue was added MeCN to afford a precipitate. The precipitate was filtered off and dried in vacuum to afford intermediate i-29a as a white solid.

(299) .sup.1H NMR (400 MHz, DMSO-d6) δ=8.09 (m, 1H), 7.80-7.76 (m, 1H), 7.72-7.67 (m, 1H), 7.43 (m, 1H), 5.15 (br s, 1H), 1.44 (s, 6H) ppm.

(300) LCMS (ESI) m/z: [M−H.sub.2O+H].sup.+=163.1.

Step 2: Preparation of 3-(2-hydroxypropan-2-yl)-N-(2-oxo-2-((4-phenylthiazol-2-yl)amino)ethyl)benzamide (Compound 61)

(301) ##STR00395##

(302) To a solution of intermediate i-29a (25.94 mg, 143.96 μmol) in DCM (1 mL) were added HATU (82.11 mg, 215.94 μmol) and DIPEA (93.03 mg, 719.80 μmol). The reaction mixture was stirred at 25° C. for 0.5 hr. Then 2-amino-N-(4-phenylthiazol-2-yl)acetamide (50 mg, 143.96 μmol, TFA salt) was added. The reaction mixture was stirred at 25° C. for 2 hr. The reaction mixture was diluted with MeCN (2 mL) and a white precipitate formed. The precipitate was filtered off and lyophilized to afford compound 61 as a white solid.

(303) LCMS (ESI) m/z: [M+H].sup.+=396.3.

(304) .sup.1H NMR (400 MHz, DMSO-d6) δ=8.93-8.90 (m, 1H), 8.01 (s, 1H), 7.90 (d, J=7.6 Hz, 2H), 7.73 (d, J=8.0 Hz, 1H), 7.65 (d, J=8.0 Hz, 1H), 7.63 (s, 1H), 7.45-7.40 (m, 3H), 7.35-7.30 (m, 1H), 5.14 (s, 1H), 4.19 (d, J=5.6 Hz, 2H), 1.46 (s, 6H) ppm.

Example 30. Preparation of (S)-3-(tert-butyl)-N-(4-(methylthio)-1-oxo-1-((4-phenylthiazol-2-yl)amino)butan-2-yl)benzamide (Compound 73)

(305) ##STR00396##

(306) To a solution of 3-tert-butylbenzoic acid (21.14 mg, 118.64 μmol), DIPEA (82.66 μL) in DCM (0.5 mL) was added HATU (67.66 mg, 177.96 μmol) at 0° C. After addition, the mixture was stirred at this temperature for 15 min, and then (2S)-2-amino-4-methylsulfanyl-N-(4-phenylthiazol-2-yl) butanamide (50 mg, 118.64 μmol) was added at 0° C. The resulting mixture was stirred at 20° C. for 2 hr. The reaction mixture was diluted with water (1 mL) and extracted with DCM (2*1 mL). The combined organic extracts were washed with brine (2 mL), dried over anhydrous Na.sub.2SO.sub.4, filtered and concentrated under reduced pressure to give the residue. The residue was purified by prep-HPLC (combiflash, CH.sub.3CN/H.sub.2O, 5-95%, FA condition) to give compound 73 as a white solid.

(307) LCMS (ESI) m/z: [M+H].sup.+=468.3.

(308) .sup.1H NMR (400 MHz, DMSO-d6) δ=12.62-12.30 (br s, 1H), 8.73 (d, J=7.2 Hz, 1H), 7.91-7.89 (m, 3H), 7.76 (d, J=8.0 Hz, 1H), 7.63 (s, 1H), 7.62-7.57 (m, 1H), 7.43-7.41 (m, 3H), 7.35-7.30 (m, 1H), 4.79-4.74 (m, 1H), 2.66-2.50 (m, 2H), 2.14-2.12 (m, 2H), 2.10 (s, 3H), 1.32 (s, 9H) ppm.

Example 31. Preparation of (S)-3-(2-hydroxypropan-2-yl)-N-(4-(methylthio)-1-oxo-1-((4-phenylthiazol-2-yl)amino)butan-2-yl)benzamide (Compound 55)

(309) ##STR00397##

(310) To a solution of 3-(1-hydroxy-1-methyl-ethyl)benzoic acid (21.38 mg, 118.64 μmol), DIPEA (82.66 μL) in DCM (0.5 mL) was added HATU (67.66 mg, 177.96 μmol) at 0° C. The mixture was stirred at this temperature for 15 min, and then (2S)-2-amino-4-methylsulfanyl-N-(4-phenylthiazol-2-yl)butanamide (50 mg, 118.64 μmol) was added at 0° C. The resulting mixture was stirred at 20° C. for 12 hr. The reaction mixture was diluted with water (1 mL) and extracted with DCM (2*1 mL). The combined organic extracts were washed with brine (2 mL), dried over anhydrous Na.sub.2SO.sub.4, filtered and concentrated under reduced pressure to give the residue. The residue was purified by reverse phase-HPLC (FA condition) to give compound 55 as a white solid.

(311) .sup.1H NMR (400 MHz, DMSO-d6) δ=12.48 (s, 1H), 8.71 (br d, J=7.2 Hz, 1H), 8.00 (s, 1H), 7.90 (d, J=7.6 Hz, 2H), 7.77 (d, J=8.0 Hz, 1H), 7.65-7.62 (m, 2H), 7.45-7.41 (m, 3H), 7.33-7.32 (m, 1H), 5.11 (s, 1H), 4.78-4.77 (m, 1H), 2.66-2.56 (m, 2H), 2.16-2.12 (m, 2H), 2.10 (s, 3H), 1.45 (s, 6H) ppm.

(312) LCMS (ESI) m/z: [M+H].sup.+=470.1.

Example 32. Preparation of 4-methyl-N-[(1S)-3-methylsulfanyl-1-[(4-phenylthiazol-2-yl)carbamoyl]propyl]-3-methylsulfonyl-benzamide (Compound 2)

(313) ##STR00398##

Step 1: Preparation 4-methyl-3-methylsulfonyl-benzoic acid (Intermediate i-32a)

(314) ##STR00399##

(315) To a mixture of methyl 4-methyl-3-methylsulfonyl-benzoate (100 mg, 438.09 μmol) in THF (1 mL), MeOH (1 mL) and H.sub.2O (0.5 mL) was added LiOH.H.sub.2O (55.15 mg, 1.31 mmol) and the mixture was stirred at 30° C. for 2 hr. Water (20 mL) was added and pH adjusted to 4 with 2N HCl and then the mixture was extracted with EtOAc (20 mL*2). The combined organic phase was washed with brine, dried over anhydrous Na.sub.2SO.sub.4, filtered and concentrated under vacuum to give intermediate i-32a as a white solid which was used in the next step without further purification.

(316) LCMS (ESI) m/z: [M+H].sup.+=215.2.

Step 2: Preparation of 4-methyl-N-[(1S)-3-methylsulfanyl-1-[(4-phenylthiazol-2-yl)carbamoyl]propyl]-3-methylsulfonyl-benzamide (Compound 2)

(317) ##STR00400##

(318) To a mixture of intermediate i-32a (20.33 mg, 94.91 μmol) in DCM (1 mL) was added HATU (43.30 mg, 113.89 μmol) and DIEA (36.80 mg, 284.73 μmol) and the mixture was stirred at 15° C. for 5 min. Then (2S)-2-amino-4-methylsulfanyl-N-(4-phenylthiazol-2-yl)butanamide (40 mg, 94.91 μmol, TFA salt) was added and the mixture was stirred at 15° C. for 16 hr. The solvent was removed under reduced pressure and then the residue was purified through Prep.HPLC (column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 42%-72%, 9 min), and lyophilized to give compound 2 as a white solid

(319) .sup.1H NMR (400 MHz, MeOD) δ=8.53 (d, J=1.6 Hz, 1H), 8.11-8.09 (m, 1H), 7.91-7.89 (m, 2H), 7.57 (d, J=8.0, 1H), 7.40-7.37 (m, 3H), 7.31-7.27 (m, 1H), 4.93-4.91 (m, 1H), 3.19 (s, 3H), 2.77 (s, 3H), 2.72- 2.61 (m, 2H), 2.33-2.18 (m, 2H), 2.14 (s, 3H) ppm.

(320) LCMS (ESI) m/z: [M+H].sup.+=504.2.

Example 33. Preparation of methyl 3-[2-[[2-[[3-(dimethylsulfamoyl)benzoyl]amino]acetyl]amino]thiazol-4-yl]benzoate (Compound 9)

(321) ##STR00401##

(322) To a solution of N-[2-[(4-bromothiazol-2-yl)amino]-2-oxo-ethyl]-3-(dimethylsulfamoyl)benzamide (30 mg, 67.07 μmol) and (3-methoxycarbonylphenyl)boronic acid (36.21 mg, 201.21 μmol) in 1,4-dioxane (3 mL) and H.sub.2O (0.2 mL) were added Pd(t-Bu.sub.3P).sub.2 (17.14 mg, 33.53 μmol) and DIEA (43.34 mg, 335.35 μmol, 58.41 μL) under N.sub.2. The mixture was stirred at 100° C. for 2 hrs then poured into H.sub.2O (20 mL) and extracted with EtOAc (5 mL*3). The combined organic extracts were washed with H.sub.2O (5 mL*2) and brine (5 mL*1), dried over Na.sub.2SO.sub.4, filtered and concentrated under vacuum. The residue was purified through Prep-HPLC (column: Phenomenex Gemini 150*25 mm*10 μm; mobile phase: [water (0.04% NH.sub.3H.sub.2O+10 mM NH.sub.4HCO.sub.3)-ACN]; B %: 36%-66%, 10 min) and lyophilized to compound 9 as a white solid.

(323) .sup.1H NMR (400 MHz, DMSO-d6) δ 12.58 (s, 1H), 9.33-9.31 (m, 1H), 8.56 (s, 1H), 8.27-8.24 (m, 2H), 8.18 (d, J=8.0 Hz, 1H), 7.94-7.91 (m, 2H), 7.83-7.81 (m, 2H), 7.62-7.60 (m, 1H), 4.25 (d, J=5.6 Hz, 2H), 3.89 (s, 3H), 2.66 (s, 6H) ppm

(324) LCMS (ESI) m/z: [M+H].sup.+=503.3.

Example 34. Preparation of 3-[2-[[2-[[3-(dimethylsulfamoyl)benzoyl]amino]acetyl]amino]thiazol-4-yl]-N-methyl-benzamide (Compound 4)

(325) ##STR00402##

(326) To a solution of N-[2-[(4-bromothiazol-2-yl)amino]-2-oxo-ethyl]-3-(dimethylsulfamoyl)benzamide (30 mg, 67.07 μmol) and [3-(methylcarbamoyl)phenyl]boronic acid (36.01 mg, 201.21 μmol) in 1,4-dioxane (3 mL) and H.sub.2O (0.15 mL) were added Pd(t-Bu.sub.3P).sub.2 (17.14 mg, 33.53 μmol) and DIEA (43.34 mg, 335.35 μmol, 58.41 μL) under N.sub.2. The mixture was stirred at 100° C. for 2 hrs then poured into H.sub.2O (20 mL) and extracted with EtOAC (5 mL*3). The combined organic layers were washed with H.sub.2O (5 mL*2) and brine (5 mL), dried over Na.sub.2SO.sub.4, filtered and concentrated under vacuum. The residue was purified by Prep-HPLC (column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 35%-65%, 9 min) and lyophilized to give compound 4 as a white solid.

(327) .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ=12.55 (s, 1H), 9.33-9.30 (m, 1H), 8.52 (s, 1H), 8.40 (s, 1H), 8.27-8.24 (m, 2H), 8.02 (d, J=7.6 Hz, 1H), 7.94 (d, J=7.6 Hz, 1H), 7.81-7.76 (m, 3H), 7.53-7.51 (m, 1H), 4.25 (d, J=5.6 Hz, 2H), 2.81 (d, J=4.4 Hz, 3H), 2.66 (s, 6H), ppm.

(328) LCMS (ESI) m/z: [M+H].sup.+=502.3.

Example 35. Preparation of N-[2-[[4-(2-bromophenyl)thiazol-2-yl]amino]-2-oxo-ethyl]-3-(dimethylsulfamoyl)benzamide (Compound 18)

(329) ##STR00403##

Step 1: Preparation of tert-butyl N-[2-[[4-(2-bromophenyl)thiazol-2-yl]amino]-2-oxo-ethyl]carbamate (Intermediate i-35a)

(330) ##STR00404##

(331) To a solution of 2-(tert-butoxycarbonylamino)acetic acid (82.40 mg, 470.34 μmol) in DCM (10 mL) were added HATU (223.55 mg, 587.93 μmol) and DIEA (151.97 mg, 1.18 mmol) and the mixture was stirred at 30° C. for 30 min. Then 4-(2-bromophenyl) thiazol-2-amine (100 mg, 391.95 μmol) was added. The mixture was stirred at 30° C. for another 12 hrs and then diluted with DCM (50.0 mL) and washed with H.sub.2O (10.0 mL*3) and brine (10.0 mL*2), then dried over Na.sub.2SO.sub.4, filtered and concentrated under vacuum to give intermediate i-35a as yellow oil which was used in the next step directly.

(332) LCMS (ESI) m/z: [M+H].sup.+=412.3.

Step 2: Preparation of 2-amino-N-[4-(2-bromophenyl)thiazol-2-yl]acetamide (Intermediate i-35b) trifluoroacetate salt

(333) ##STR00405##

(334) To a solution of intermediate i-35a (150 mg, 363.81 μmol) in DCM (5.0 mL) was added TFA (1.0 mL). The solution was stirred at 30° C. for 2 hr. Then the mixture was diluted with DCM (20.0 mL) and concentrated under vacuum. This operation was repeated three times. The residue was washed by MTBE (5.0 mL*2) and dried in vacuum to give intermediate i-35b (TFA salt) as yellow oil which was used in the next step directly.

(335) LCMS (ESI) m/z: [M+H].sup.+=312.3

Step 3: Preparation of N-[2-[[4-(2-bromophenyl)thiazol-2-yl]amino]-2-oxo-ethyl]-3-(dimethylsulfamoyl)benzamide (Compound 18)

(336) ##STR00406##

(337) To a solution of 3-(dimethylsulfamoyl)benzoic acid (96.82 mg, 422.33 μmol) in DMF (3.0 mL) were added HATU (401.46 mg, 1.06 mmol) and DIEA (227.43 mg, 1.76 mmol, 306.51 μL) and the mixture was stirred at 30° C. for 30 min. Then intermediate i-35b (150 mg, 351.94 μmol) was added. The mixture was stirred at 30° C. for 16 hrs, then poured into H.sub.2O (30.0 mL) and extracted with EtOAc (5.0 mL*3). The combined organic extracts were washed with H.sub.2O (5.0 mL*2) and brine (5.0 mL), then dried over Na.sub.2SO.sub.4, filtered and concentrated under vacuum. The residue was purified by Prep-HPLC (column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 42%-72%, 9 min) and lyophilized to give compound 18 as a light yellow solid.

(338) .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ=12.51 (s, 1H), 9.34-9.31 (m, 1H), 8.27-8.23 (m, 2H), 7.94 (d, J=8.0 Hz, 1H), 7.83-7.81 (m, 1H), 7.75-7.69 (m, 2H), 7.54 (s, 1H), 7.48-7.46 (m, 1H), 7.32-7.30 (m, 1H), 4.24 (d, J=5.6 Hz, 2H), 2.66 (s, 6H) ppm

(339) LCMS (ESI) m/z: [M+H].sup.+=525.0.

Example 36. Preparation of (N-[2-[[4-(3-bromophenyl)thiazol-2-yl]amino]-2-oxo-ethyl]-3-(dimethylsulfamoyl)benzamide (Compound 7)

(340) ##STR00407##

Step 1: Preparation of tert-butyl N-[2-[[4-(3-bromophenyl)thiazol-2-yl]amino]-2-oxo-ethyl]carbamate (Intermediate i-36a)

(341) ##STR00408##

(342) To a solution of 2-(tert-butoxycarbonylamino)acetic acid (82.40 mg, 470.34 μmol) in DCM (10 mL) were added HATU (223.55 mg, 587.93 μmol) and DIEA (151.97 mg, 1.18 mmol, 204.81 μL) and the mixture was stirred at 30° C. for 30 min. Then 4-(3-bromophenyl)thiazol-2-amine (100.00 mg, 391.95 μmol) was added. The mixture was stirred at 30° C. for another 12 hrs then diluted with DCM (50.0 mL). The solution was washed with H.sub.2O (10.0 mL*3) and brine (10 mL*2), then dried over Na.sub.2SO.sub.4, filtered and concentrated under vacuum to give intermediate i-36a as yellow oil which was used directly in the next step.

(343) LCMS (ESI) m/z: [M+H].sup.+=412.3

Step 2: Preparation of 2-amino-N-[4-(3-bromophenyl)thiazol-2-yl]acetamide (Intermediate i-36b) trifluoroacetate salt

(344) ##STR00409##

(345) To a solution of intermediate i-36a (150.00 mg, 363.81 μmol) in DCM (5 mL) was added TFA (1 mL), the solution was stirred at 30° C. for 2 hrs. Then the mixture was diluted with DCM (20.0 mL) and concentrated in vacuum. This operation was repeated three times. The residue was washed by MTBE (5.0 mL*2) and dried in vacuum to give intermediate i-36b (TFA salt) as yellow oil which was used directly in the next step.

(346) LCMS (ESI) m/z: [M+H].sup.+=312.3

Step 3: Preparation of (N-[2-[[4-(3-bromophenyl)thiazol-2-yl]amino]-2-oxo-ethyl]-3-(dimethylsulfamoyl)benzamide (Compound 7)

(347) ##STR00410##

(348) To a solution of 3-(dimethylsulfamoyl)benzoic acid (96.82 mg, 422.33 μmol) in DMF (3.0 mL) were added HATU (401.46 mg, 1.06 mmol) and DIEA (227.43 mg, 1.76 mmol, 306.51 μL) and the mixture was stirred at 30° C. for 30 min. Then intermediate i-36b (150 mg, 351.94 μmol) was added. The mixture was stirred at 30° C. for 16 hrs then poured into H.sub.2O (30.0 mL) and extracted with EtOAc (5.0 mL*3). The combined organic extracts were washed with H.sub.2O (5.0 mL*2) and brine (5 mL), then dried over Na.sub.2SO.sub.4, filtered and concentrated under vacuum. The residue was purified by Pre-HPLC (column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 42%-72%, 9 min) and lyophilized to give compound 7 as a pink solid.

(349) .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ=12.51 (s, 1H), 9.33-9.30 (m, 1H), 8.27-8.23 (m, 2H), 8.11 (s, 1H), 7.94- 7.91 (m, 2H), 7.82-7.80 (m, 2H), 7.43-7.42 (m, 1H), 7.41-7.40 (m, 1H), 4.24 (d, J=5.6 Hz, 2H), 2.66 (s, 6H) ppm

(350) LCMS (ESI) m/z: [M+H].sup.+=523.2.

Example 37. Preparation of Compound (S)—N-(4-(methylthio)-1-oxo-1-((4-phenylthiazol-2-yl)amino)butan-2-yl)nicotinamide (Compound 70)

(351) ##STR00411##

Step 1: Preparation of (S)-tert-butyl (4-(methylthio)-1-oxo-1-((4-phenylthiazol-2-yl)amino)butan-2-yl)carbamate (Intermediate i-37a)

(352) ##STR00412##

(353) To a solution of (2S)-2-(tert-butoxycarbonylamino)-4-methylsulfanyl-butanoic acid (5.0 g, 20.05 mmol) in DCM (20.0 mL) was added EEDQ (6.20 g, 25.07 mmol), then 4-phenylthiazol-2-amine (2.95 g, 16.71 mmol) was added to the mixture. The mixture was stirred at 25° C. for 3 hr. 10% of Citric acid (800.0 mL) was added and the reaction mixture was extracted with EtOAc (200 mL). The combined organic extracts were washed with brine (100 mL), dried over Na.sub.2SO.sub.4, filtered and concentrated under reduced pressure to give a residue. The residue was purified by column chromatography (SiO.sub.2, Petroleum ether/Ethyl acetate=50/1 to 8:1) to give intermediate i-37a as a white solid.

(354) LCMS (ESI) m/z: [M+H].sup.+=408.0.

(355) SFC HPLC: Amycoat-MeOH(DEA)-5-40-3 mL-35, 2.284 min.

Step 2: Preparation of (S)-2-amino-4-(methylthio)-N-(4-phenylthiazol-2-yl) butanamide (Intermediate i-37b) trifluoroacetate salt

(356) ##STR00413##

(357) To a solution of intermediate i-37a (5.8 g, 14.23 mmol) in DCM (20.0 mL) was added TFA (4.0 mL). The mixture was stirred at 10° C. for 2 hr. The reaction mixture was concentrated under reduced pressure. The crude product intermediate i-37b (TFA salt) was lyophilized and obtained as a white solid. This material was used in the next step without further purification

(358) LCMS (ESI) m/z: [M+H].sup.+=307.9.

(359) SFC HPLC: Cellucoat-MeOH(DEA)-10-12 min-3, 8.970 min.

Step 3: Preparation of (S)—N-(4-(methylthio)-1-oxo-1-((4-phenylthiazol-2-yl)amino)butan-2-yl)nicotinamide (Compound 70)

(360) ##STR00414##

(361) To a solution of nicotinic acid (14.61 mg, 118.64 μmol) in DCM (2 mL) was added HATU (67.66 mg, 177.95 μmol) and DIPEA (61.33 mg, 474.54 μmol, 82.66 μL), then intermediate i-37b (50 mg, 118.64 μmol) was added. The mixture was stirred at 10° C. for 2 hr. The reaction mixture was concentrated under reduced pressure. The residue was purified by prep-HPLC (Phenomenex Gemini 150*25 mml 0 μm; mobile phase: [water (10 mM NH4HCO3)-ACN]; B %: 45%-75%, 10 min) and lyophilized to give compound 70 as a white solid.

(362) 1H NMR (400 MHz, METHANOL-d4) δ 9.06 (m, 1H), 8.72-8.71 (m, 1H), 8.33-8.31 (m, 1H), 7.91-7.89 (m, 2H), 7.58-7.55 (m, 1H), 7.40-7.38 (m, 3H), 7.37-7.30 (m, 1H), 4.94-4.91 (m, 1H), 2.75-2.65 (m, 2H), 2.31-2.29 (m, 2H), 2.14 (s, 3H) ppm.

(363) LCMS (ESI) m/z: [M+H].sup.+=413.1.

(364) Chiral HPLC: Cellucoat-MeOH(DEA)-5-40-3 mL-35T.Icm, 2.269 min.

Example 38. Preparation of 4-methoxy-N-[(1S)-3-methylsulfanyl-1-[(4-phenylthiazol-2-yl)carbamoyl]propyl]benzamide (Compound 67)

(365) ##STR00415##

(366) To a solution of 4-methoxybenzoic acid (21.66 mg, 142.36 μmol) in DCM (2.0 mL) was added HATU (90.22 mg, 237.27 μmol) and DIEA (76.66 mg, 593.18 μmol, 103.32 μL). The mixture was stirred at 30° C. for 30 min. Then (2S)-2-amino-4-methylsulfanyl-N-(4-phenylthiazol-2-yl) butanamide (50 mg, 118.64 μmol) was added. The mixture was stirred at 30° C. for another 2 hrs. The reaction solution was diluted with DCM (20.0 mL) and washed with (5.0 mL*3) and brine (5 mL), dried over Na.sub.2SO.sub.4, filtered and concentrated under vacuum. The residue was purified by Pre-HPLC (column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 45%-75%, 9 min) and lyophilized to give compound 67 as a white solid.

(367) .sup.1HNMR (400 MHz, METHANOL-d4) δ 7.92-7.90 (m, 4H), 7.42-7.38 (m, 3H), 7.33-7.29 (m, 1H), 7.03 (d, J=9.2 Hz, 2H), 4.94-4.91 (m, 1H), 3.88 (s, 3H), 2.75-2.65 (m, 2H), 2.36-2.15 (m, 2H), 2.14 (s, 3H) ppm.

(368) LCMS (ESI) m/z: [M+H].sup.+=442.1.

Example 39. Preparation of 2-hydroxy-N-[(1S)-3-methylsulfanyl-1-[(4-phenylthiazol-2-yl)carbamoyl]propyl]benzamide (Compound 69)

(369) ##STR00416##

(370) To a mixture of (2S)-2-amino-4-methylsulfanyl-N-(4-phenylthiazol-2-yl)butanamide (50 mg, 118.64 μmol) and 2-hydroxybenzoic acid (16.39 mg, 118.64 μmol) in DCM (1 mL) was added DIPEA (61.33 mg, 474.54 μmol). The mixture was stirred at 30° C. for 15 min, then HATU (67.66 mg, 177.95 μmol) was added and stirred at 30° C. for 2 hours. The reaction mixture was evaporated to dryness to give the crude product. The residue was purified by prep-HPLC (column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 51%-71%, 12 min) and lyophilized to give compound 69 as a white solid.

(371) .sup.1H NMR (400 MHz, MeOD-d.sub.4) δ=7.92-7.89 (m, 3H), 7.43-7.37 (m, 4H), 7.31-7.28 (m, 1H), 6.96-6.92 (m, 2H), 4.98-4.94 (m, 1H), 2.72-2.60 (m, 2H), 2.35-2.27 (m, 1H), 2.25-2.17 (m, 1H), 2.13 (s, 3H) ppm.

(372) LCMS (ESI) m/z: [M+H].sup.+=428.1.

(373) Chiral HPLC: OD-3_5CM_MEOH(DEA)_40_3ML_T35.M, 0.822 min.

Example 40. Preparation of Compound (S)-3-hydroxy-N-(4-(methylthio)-1-oxo-1-((4-phenylthiazol-2-yl)amino)butan-2-yl)benzamide (Compound 59)

(374) ##STR00417##

(375) To a solution of 3-hydroxybenzoic acid (16.39 mg, 118.64 μmol, 9.94 μL) and (2S)-2-amino-4-methylsulfanyl-N-(4-phenylthiazol-2-yl)butanamide (50 mg, 118.64 μmol) in DCM (2.0 mL) was added DIPEA (61.33 mg, 474.56 μmol), then HATU (67.66 mg, 177.96 μmol) was added. The mixture was stirred at 10° C. for 2 hr. The reaction mixture was concentrated under reduced pressure. The residue was purified by prep-HPLC (TFA condition; column: column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 45%-75%, 9 min) and lyophilized to give compound 59 as a white solid.

(376) .sup.1HNMR (400 MHz, METHANOL-d4) δ=7.90-7.88 (m, 2H) 7.38-7.34 (m, 4H), 7.30-7.27 (m, 3H), 6.98-6.95 (m, 1H), 4.90-4.87 (m, 1H), 2.69-2.62 (m, 2H), 2.26-2.20 (m, 2H), 2.13 (s, 3H) ppm LCMS (ESI) m/z: [M+H].sup.+=428.1.

(377) Chiral HPLC: AS-3_5CM_MEOH(DEA)_40_3ML_5MIN_T35.M, 1.341 min.

Example 41. Preparation of 4-hydroxy-N-[(1S)-3-methylsulfanyl-1-[(4-phenylthiazol-2-yl)carbamoyl]propyl]benzamide (Compound 53)

(378) ##STR00418##

(379) To a solution of 4-hydroxybenzoic acid (16.39 mg, 118.64 μmol) and (2S)-2-amino-4-methyl sulfanyl-N-(4-phenylthiazol-2-yl)butanamide (50 mg, 118.64 μmol) in DCM (2.0 mL) were added DIEA (76.66 mg, 593.18 μmol, 103.32 μL) and HATU (90.22 mg, 237.27 μmol). The mixture was stirred at 30° C. for 2.5 hrs. The reaction was diluted with DCM (20.0 mL) and washed with water (5.0 mL*3) and brine (5.0 mL), dried over Na.sub.2SO.sub.4, filtered and concentrated under vacuum to give crude product. The crude product was purified by Pre-HPLC (column: Boston pH-lex 150*25 10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 48%-68%, 8 min) and lyophilized to give compound 53 as a yellow solid.

(380) .sup.1H NMR (400 MHz, METHANOL-d.sub.4) δ=7.91 (d, J=8.4 Hz, 2H), 7.82 (d, J=8.4 Hz, 2H), 7.42-7.38 (m, 3H), 7.32-7.30 (m, 1H), 6.87-6.85 (m, 2H), 4.90-4.86 (m, 1H), 2.72-2.64 (m, 2H), 2.28-2.21 (m, 2H), 2.15 (s, 3H) ppm.

(381) LCMS (ESI) m/z: [M+H].sup.+=428.3.

Example 42. Preparation of 2-amino-N-[(1S)-3-methylsulfanyl-1-[(4-phenylthiazol-2-yl)carbamoyl]propyl]benzamide (Compound 63)

(382) ##STR00419##

(383) To a mixture of 2-aminobenzoic acid (16.27 mg, 118.64 μmol) and (2S)-2-amino-4-methyl sulfanyl-N-(4-phenylthiazol-2-yl)butanamide (50 mg, 118.64 μmol) in DCM (1 mL) was added DIPEA (61.33 mg, 474.54 μmol). The mixture was stirred at 30° C. for 15 min, then HATU (67.66 mg, 177.95 μmol) was added and stirred at 30° C. for 2 hours. The reaction mixture was evaporated to dryness to give the crude product. The residue was purified by prep-HPLC (column: Boston pH-lex 150*25 10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 55%-75%, 8 min) and lyophilized to give compound 63 (TFA salt) as a white solid.

(384) .sup.1H NMR (400 MHz, MeOD-d.sub.4) δ=7.91-7.89 (m, 2H), 7.71-7.69 (m, 1H), 7.41-7.37 (m, 3H), 7.35-7.28 (m, 2H), 6.93-6.86 (m, 2H), 4.91-4.87 (m, 1H), 2.75-2.61 (m, 2H), 2.33-2.26 (m, 1H), 2.25-2.16 (m, 1H), 2.15 (s, 3H) ppm; LCMS (ESI) m/z: [M+H].sup.+=427.3.

(385) Chiral HPLC: Cellucoat-MeOH(DEA)-40-3 mL-35T.1 cm, 1.540 min.

Example 43. Preparation of Compound (S)-3-amino-N-(4-(methylthio)-1-oxo-1-((4-phenylthiazol-2-yl)amino)butan-2-yl)benzamide (Compound 56)

(386) ##STR00420##

Step 1: Preparation of (S)-tert-butyl (3-((4-(methylthio)-1-oxo-1-((4-phenylthiazol-2-yl)amino)butan-2-yl)carbamoyl)phenyl)carbamate (Intermediate i-42a)

(387) ##STR00421##

(388) To a solution of 3-(tert-butoxycarbonylamino)benzoic acid (28.15 mg, 118.65 μmol) in DCM (2.0 mL) were added HATU (67.66 mg, 177.94 μmol) and DIPEA (61.33 mg, 474.54 μmol), then (2S)-2-amino-4-methylsulfanyl-N-(4-phenylthiazol-2-yl)butanamide (50 mg, 118.64 μmol) was added. The mixture was stirred at 10° C. for 12 hr. The reaction mixture was concentrated under reduced pressure. The residue was purified by prep-HPLC (TFA condition; column: column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 55%-85%, 9 min) to give intermediate i-42a as a white solid. LCMS (ESI) m/z: [M+H].sup.+=527.1.

Step 2: Preparation of (S)-3-amino-N-(4-(methylthio)-1-oxo-1-((4-phenylthiazol-2-yl)amino)butan-2-yl)benzamide (Compound 56) trifluoroacetate salt

(389) ##STR00422##

(390) To a solution of intermediate i-42a (20 mg, 37.97 μmol) in DCM (2.0 mL) was added TFA (1.0 mL). The mixture was stirred at 10° C. for 2 hr. The reaction mixture was concentrated under reduced pressure. The residue was purified by prep-HPLC (TFA condition; column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 35%-65%, 9 min) and lyophilized to give compound 56 (TFA salt) as a white solid.

(391) 1H NMR (400 MHz, METHANOL-d4) 6=7.90-7.88 (m, 2H), 7.84-7.83 (m, 1H), 7.72-7.71 (m, 1H), 7.57-7.55 (m, 1H), 7.40-7.36 (m, 4H), 7.31-7.279 (m, 1H), 4.93-4.91 (m, 1H), 2.70-2.64 (m, 2H), 2.30-2.20 (m, 2H), 2.14 (s, 3H) ppm.

(392) LCMS (ESI) m/z: [M+H].sup.+=427.1.

Example 44. Preparation of 4-amino-N-[(1S)-3-methylsulfanyl-1-[(4-phenylthiazol-2-yl)carbamoyl]propyl]benzamide (Compound 52)

(393) ##STR00423##

(394) To a solution of 4-aminobenzoic acid (19.52 mg, 142.36 μmol) in DCM (2 mL) were added HATU (90.22 mg, 237.27 μmol) and DIEA (76.66 mg, 593.18 μmol, 103.32 μL) and the mixture was stirred at 30° C. for 30 min. Then (2S)-2-amino-4-methylsulfanyl-N-(4-phenylthiazol-2-yl) butanamide (50 mg, 118.64 μmol) was added. The mixture was stirred at 30° C. for another 2 hrs then diluted with DCM (20.0 mL). The organic layer was washed with (5.0 mL*3) and brine (5 mL), dried over Na.sub.2SO.sub.4, filtered and concentrated under vacuum. The residue was purified by Pre-HPLC (column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.225% FA)-ACN]; B %: 40%-70%, 9 min) and lyophilized to give compound 52 (TFA salt) as a white solid.

(395) .sup.1H NMR (400 MHz, METHANOL-d4) δ 7.92-7.89 (m, 2H), 7.76-7.74 (m, 2H), 7.42-7.38 (m, 3H), 7.33-7.31 (m, 1H), 6.80 (d, J=8.8 Hz, 2H), 4.94-4.88 (m, 1H), 2.71-2.64 (m, 2H), 2.28-2.21 (m, 2H), 2.15 (s, 3H) ppm.

(396) LCMS (ESI) m/z: [M+H].sup.+=427.1.

Example 45. Preparation of (S)—N-(3-amino-1-oxo-1-((4-phenylthiazol-2-yl)amino)propan-2-yl)-3-(N,N-dimethylsulfamoyl)benzamide (Compound 25)

(397) ##STR00424##

Step 1: Preparation of (S)-3-((((9H-fluoren-9-yl)methoxy)carbonyl)amino)-2-((tert-butoxycarbonyl)amino)propanoic acid (Intermediate i-45a)

(398) ##STR00425##

(399) To a solution of (2S)-3-amino-2-(tert-butoxycarbonylamino)propanoic acid (1.0 g, 4.90 mmol) in THF (10.0 mL) and water (10.0 mL) were added FmocOSu (1.82 g, 5.39 mmol) and NaHCO.sub.3 (822.69 mg, 9.79 mmol). The mixture was stirred at 20° C. for 3 hours. The reaction mixture was adjusted to pH 4 with citric acid solution. The solution was extracted with EtOAc (10.0 mL*3). The organic layer was washed with brine (20.0 mL), dried over Na.sub.2SO.sub.4 and filtered. The filtrate was concentrated to give a residue. The residue was triturated with MTBE (10.0 mL). The solution was filtered and the filter cake was dried under vacuum to give intermediate i-45a as a white solid.

(400) LCMS (ESI) m/z: [M+Na].sup.+=449.3

Step 2: Preparation of (S)-(9H-fluoren-9-yl)methyl tert-butyl (3-oxo-3-((4-phenylthiazol-2-yl)amino)propane-1,2-diyl)dicarbamate (Intermediate i-45b)

(401) ##STR00426##

(402) To a solution of intermediate i-45a (1.8 g, 4.22 mmol) in DCM (18.0 mL) were added EEDQ (1.57 g, 6.33 mmol) and 4-phenylthiazol-2-amine (743.86 mg, 4.22 mmol). The reaction mixture was stirred at 20° C. for 12 hours and then poured into water (20.0 mL). The solution was extracted with EtOAc (20.0 mL*3). The combine organic layers were washed with brine (50.0 mL), dried over Na.sub.2SO.sub.4 and filtered. The filtrate was concentrated to give a residue. The residue was purified by column chromatography (SiO.sub.2, PE:EA=100:1-50:1-10:1-5:1-2:1) to give intermediate i-45b as a yellow solid.

(403) LCMS (ESI) m/z: [M+H].sup.+=585.4

(404) Chiral HPLC: Cellucoat-MeOH(DEA)-40-7MIN-3ML, 3.031 min.

Step 3: Preparation of (S)-(9H-fluoren-9-yl)methyl (2-amino-3-oxo-3-((4-phenylthiazol-2-yl)amino)propyl)carbamate (Intermediate i-45c)

(405) ##STR00427##

(406) To a solution of intermediate i-45b (2.5 g, 4.28 mmol) in DCM (25.0 mL) was added TFA (4.0 mL). The reaction mixture was stirred at 20° C. for 1.5 hours. The reaction mixture was concentrated to give a residue. The residue was triturated with PE (10.0 mL). Then the mixture was filtered and the solid was dried in vacuum to give intermediate i-45c (TFA salt) as a white solid.

(407) LCMS (ESI) m/z: [M+Na].sup.+=507.3

(408) Chiral HPLC: OJ-3-MeOH(DEA)-60-7MIN-3ML-35T, 5.436 min

Step 4: Preparation of (S)-(9H-fluoren-9-yl)methyl (2-(3-(N,N-dimethylsulfamoyl)benzamido)-3-oxo-3-((4-phenylthiazol-2-yl)amino)propyl)carbamate (Intermediate i-45c)

(409) ##STR00428##

(410) To a solution of 3-(dimethylsulfamoyl)benzoic acid (38.30 mg, 167.06 μmol) in DCM (1.0 mL) were added HATU (69.87 mg, 183.76 μmol), DIEA (64.77 mg, 501.18 μmol, 87.30 μL) and intermediate i-45b (100 mg, 167.06 μmol). The reaction mixture was stirred at 20° C. for 12 hours and then poured into water (2.0 mL). The solution was extracted with EtOAc (2.0 mL*3). The combined organic extracts were washed with brine (5.0 mL), dried over Na.sub.2SO.sub.4 and filtered. The filtrate was concentrated to give a residue. The residue was purified by column chromatography (SiO.sub.2. PE/EA=50/1-1/1) to give intermediate i-45c as a white solid.

(411) LCMS (ESI) m/z: [M+H].sup.+=696.0

(412) Chiral HPLC: OJ-3-MeOH(DEA)-60-7MIN-3ML-35T, 5.276 min.

Step 5: Preparation of (S)—N-(3-amino-1-oxo-1-((4-phenylthiazol-2-yl)amino)propan-2-yl)-3-(N,N-dimethylsulfamoyl)benzamide (Compound 25) Formate Salt

(413) ##STR00429##

(414) To a solution of intermediate i-45c (100.0 mg, 140.84 μmol) in DCM (1.0 mL) was added piperidine (98.00 μL). The solution was stirred at 20° C. for 2 hours. The mixture was poured into water (2.0 mL), extracted with EtOAc (2.0 mL*3). The combined organic extracts were washed with brine (3.0 mL), dried over Na.sub.2SO.sub.4 and filtered. The filtrate was concentrated to give a residue. The residue was purified by Prep-HPLC (column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.225% FA)-ACN]; B %: 17%-37%, 7 min) and lyophilized to give compound 25 (FA salt) as a white solid.

(415) .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ=8.30-8.28 (m, 2H), 8.23-8.22 (m, 2H), 7.96-7.89 (m, 3H), 7.81 (d, J=8.0 Hz, 1H), 7.63 (s, 1H), 7.45-7.43 (m, 2H), 7.41-7.32 (m, 1H), 4.74 (s, 1H), 3.11 (s, 2H), 2.66 (s, 6H) ppm

(416) LCMS (ESI) m/z: [M+H].sup.+=474.3

(417) Chiral HPLC: OJ-3-MeOH (DEA)-40-7MIN-3ML-35T, 2.956 min.

(418) Chiral HPLC: AD-3_5CM_MEOH(DEA)_40_3ML_8MIN_T35.M

Example 46. Preparation of (S)—N-(3-acetamido-1-oxo-1-((4-phenylthiazol-2-yl)amino)propan-2-yl)-3-(N,N-dimethylsulfamoyl)benzamide (Compound 21)

(419) ##STR00430##

(420) The solution of N-[(1S)-1-(aminomethyl)-2-oxo-2-[(4-phenylthiazol-2-yl)amino]ethyl]-3-(dimethylsulfamoyl)benzamide (20.0 mg, 38.49 μmol) in Ac.sub.2O (0.2 mL) was stirred at 20° C. for 2 hrs. Then the reaction mixture was poured into water (2.0 mL) and extracted with EtOAc (2.0 mL*3). The combined organic extracts were washed with brine (5.0 mL), dried over Na.sub.2SO.sub.4 and filtered. The filtrate was concentrated to give a residue. The residue was lyophilized to give compound 21 (51.86% ee) as a white solid.

(421) 1H NMR (400 MHz, DMSO-d6) δ=12.54 (s, 1H), 9.12 (d, J=6.8 Hz, 1H), 8.23-8.22 (m, 3H), 7.95 (d, J=7.6 Hz, 1H), 7.89 (d, J=7.2 Hz, 2H), 7.81 (s, 1H), 7.65 (s, 1H), 7.44-7.42 (m, 2H), 7.40-7.31 (m, 1H), 4.72 (d, J=6.0 Hz, 1H), 3.62 (m, 2H), 2.65 (s, 6H), 1.83 (s, 3H) ppm

(422) LCMS (ESI) m/z: [M+H].sup.+=516.4

(423) Chiral HPLC: Amycoat-MeOH(DEA)-40-5MIN-3ML-35T, 1.514 min and 3.407 min.

Example 47. Preparation of N-(2-((4-(2-cyanophenyl)thiazol-2-yl)amino)-2-oxoethyl)-3-(N,N-dimethylsulfamoyl)benzamide (Compound 27)

(424) ##STR00431##

Step 1: Preparation tert-butyl (2-((4-(2-cyanophenyl)thiazol-2-yl)amino)-2-oxoethyl)carbamate (Intermediate i-47a)

(425) ##STR00432##

(426) To a solution of tert-butyl N-[2-[(4-bromothiazol-2-yl)amino]-2-oxo-ethyl]carbamate (200.0 mg, 558.65 μmol) in toluene (3.0 mL) were added 2-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)benzonitrile (383.93 mg, 1.68 mmol), Ad2nBuP-Pd-G3 (406.85 mg, 558.65 μmol) and K.sub.3PO.sub.4 (177.87 mg, 837.97 μmol) under N.sub.2. The reaction mixture was stirred at 60° C. for 12 hr. The reaction mixture was concentrated to give a residue. The residue was purified by column chromatography (SiO.sub.2, PE/EA=50/1˜1/1) to give intermediate i-47a as a brown solid.

(427) LCMS (ESI) m/z: [M+H].sup.+=359.2

Step 2: Preparation 2-amino-N-(4-(2-cyanophenyl)thiazol-2-yl)acetamide (Intermediate i-47b)

(428) ##STR00433##

(429) To a solution of intermediate i-47a (230 mg, 628.88 μmol) in DCM (2.5 mL) was added TFA (0.25 mL). The mixture was stirred at 30° C. for 2 hr. The reaction mixture was concentrated to give intermediate i-47b (TFA salt) as a yellow solid which was used for next step directly.

(430) LCMS (ESI) m/z: [M+Na].sup.+=281.2

Step 3: Preparation of N-(2-((4-(2-cyanophenyl)thiazol-2-yl)amino)-2-oxoethyl)-3-(N,N-dimethylsulfamoyl)benzamide (Compound 27)

(431) ##STR00434##

(432) To a solution of 3-(dimethylsulfamoyl)benzoic acid (81.41 mg, 355.13 μmol) in DCM (2.0 mL) were added HATU (162.04 mg, 426.15 μmol), DIEA (183.59 mg, 1.42 mmol) and intermediate i-47b (110.0 mg, 355.13 μmol). The reaction mixture was stirred at 30° C. for 12 hr. The reaction mixture was poured into water (5.0 mL). Then the solution was extracted with EtOAc (5.0 mL*3). The combined organic extracts were washed with brine (10.0 mL), dried over Na.sub.2SO.sub.4 and filtered. The filtrate was concentrated to give a residue. The residue was purified by Pre-HPLC (column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.225% FA)-ACN]; B %: 37%-57%, 7 min) and lyophilized to give compound 27 as a white solid.

(433) 1H NMR (400 MHz, DMSO-d.sub.6) δ=12.43 (br s, 1H), 9.32 (m, 1H), 8.26-8.25 (m, 2H), 7.97-7.92 (m, 3H), 7.80-7.79 (m, 3H), 7.56 (m, 1H), 4.26 (d, J=5.6 Hz, 2H), 2.65 (s, 6H) ppm

(434) LCMS (ESI) m/z: [M+H].sup.+=470.2

Example 48. Preparation of N-[2-[[4-[3-(aminomethyl)phenyl]thiazol-2-yl]amino]-2-oxo-ethyl]-3-(dimethylsulfamoyl)benzamide (Compound 10)

(435) ##STR00435##

(436) To a solution of N-[2-[[4-(3-cyanophenyl)thiazol-2-yl]amino]-2-oxo-ethyl]-3-(dimethylsulfamoyl)benzamide (100.0 mg, 212.98 μmol) in MeOH (4.0 mL) was added Raney-Ni (99.99 mg, 1.17 mmol) under N.sub.2. The mixture was stirred under H2 (50 psi) at 15° C. for 16 hours. The mixture was filtered and the filtrate was concentrated under vacuum to give residue. The residue was purified by prep-HPLC (TFA condition; column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 15%-45%, 9 min) and lyophilized to give compound 10 (TFA salt) as a white solid.

(437) .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ=12.48 (s, 1H), 9.34-9.31 (m, 1H), 8.27-8.18 (m, 5H), 7.97-7.91 (m, 3H), 7.83-7.79 (m, 1H), 7.62 (s, 1H), 7.53-7.49 (m, 1H), 7.42 (d, J=7.6, 1H), 4.24 (d, J=5.6 Hz, 2H), 4.08 (m, 2H), 2.66 (m, 6H) ppm.

(438) LCMS (ESI) m/z: [M+H].sup.+=474.2

Example 49. Preparation of N-[2-[[4-(3-cyanophenyl)thiazol-2-yl]amino]-2-oxo-ethyl]-3-(dimethylsulfamoyl)benzamide (Compound 8)

(439) ##STR00436##

Step 1: Preparation of 2 3-(2-aminothiazol-4-yl)benzonitrile (Intermediate i-49a)

(440) ##STR00437##

(441) To a solution of 3-(2-bromoacetyl)benzonitrile (200.00 mg, 892.64 μmol) in H.sub.2O (5.0 mL) was added thiourea (74.74 mg, 981.91 μmol). The mixture was stirred at 25° C. for 1.5 hr. The reaction mixture was diluted with water (10.0 mL) and extracted with EtOAC (35.0 mL*3). The combined organic extracts were washed with brine (20.0 mL*2), dried over Na.sub.2SO.sub.4, filtered and concentrated under reduced pressure to give intermediate i-49a as a yellow solid.

(442) LCMS (ESI) m/z: [M+H].sup.+=202.1.

Step 2: Preparation of tert-butyl N-[2-[[4-(3-cyanophenyl)thiazol-2-yl]amino]-2-oxo-ethyl]carbamate (Intermediate i-49b)

(443) ##STR00438##

(444) To a solution of 2-(tert-butoxycarbonylamino)acetic acid (129.27 mg, 737.90 μmol) in DCM (8.0 mL) were added HATU (280.57 mg, 737.90 μmol), DIEA (260.09 mg, 2.01 mmol, 350.52 μL) and intermediate i-49a (135.0 mg, 670.82 μmol). The mixture was stirred at 25° C. for 12 hr. The reaction mixture was diluted with water (10.0 mL) and extracted with EtOAc (20.0 mL*3). The combined organic extracts were washed with brine (20.0 mL*2), dried over Na.sub.2SO.sub.4, filtered and concentrated under reduced pressure to give a residue. The residue was purified by Prep-HPLC (TFA condition; column: Boston pH-lex 150*25 10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 40%-70%, 10 min) and lyophilized to give intermediate i-49b as a white solid.

(445) LCMS (ESI) m/z: [M+H].sup.+=359.4

Step 3: Preparation of 2-amino-N-[4-(3-cyanophenyl)thiazol-2-yl]acetamide (Intermediate i-49c)

(446) ##STR00439##

(447) To a solution of intermediate i-49b (93.0 mg, 259.48 μmol) in DCM (1.0 mL) was added TFA (0.1 mL). The mixture was stirred at 25° C. for 1.5 hr. The mixture was concentrated under reduced pressure to give intermediate i-49c (TFA salt) as brown oil which was used in the next step without further purification.

(448) LCMS (ESI) m/z: [M+H].sup.+=259.1.

Step 4: Preparation of N-[2-[[4-(3-cyanophenyl)thiazol-2-yl]amino]-2-oxo-ethyl]-3-(dimethylsulfamoyl)benzamide (Compound 8)

(449) ##STR00440##

(450) To a solution of 3-(dimethylsulfamoyl)benzoic acid (75.74 mg, 330.36 μmol) in DCM (2.0 mL) were added HATU (138.17 mg, 363.40 μmol), DIEA (170.79 mg, 1.32 mmol, 230.17 μL) and intermediate F (123.0 mg, 330.36 μmol. The mixture was stirred at 25° C. for 12 hr. The reaction mixture was diluted with water (10.0 mL) and extracted with EtOAc (30.0 mL*3). The combined organic extracts were washed with brine (20.0 mL*2), dried over Na.sub.2SO.sub.4, filtered and concentrated under reduced pressure to give a residue. The residue was purified by prep-HPLC (TFA condition; column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 35%-65%, 9 min) and lyophilized to give compound 8 as a white solid.

(451) .sup.1HNMR (400 MHz, DMSO-d6) δ 12.53 (s, 1H), 9.34-9.31 (m, 1H), 8.33 (s, 1H), 8.26-8.22 (m, 3H), 7.94 (d, J=8.0 Hz, 1H), 7.88 (s, 1H), 7.82-7.78 (m, 2H), 7.68-7.64 (m, 1H), 4.24 (d, J=5.6 Hz, 2H), 2.65 (s, 6H).

(452) LCMS (ESI) m/z: [M+H].sup.+=470.0.

Example 50. Preparation of N-[2-[[4-[3-(acetamidomethyl)phenyl]thiazol-2-yl]amino]-2-oxo-ethyl]-3-(dimethylsulfamoyl)benzamide (Compound 5)

(453) ##STR00441##

(454) To a solution of N-[2-[[4-(3-cyanophenyl)thiazol-2-yl]amino]-2-oxo-ethyl]-3-(dimethylsulfamoyl)benzamide (100 mg, 212.98 μmol) in MeOH (2.0 mL) were added NiCl.sub.2.6H.sub.2O (126.56 mg, 532.44 μmol) and NaBH.sub.4 (80.57 mg, 2.13 mmol), the mixture was stirred at 25° C. for 2 hr, then Ac.sub.2O (21.74 mg, 212.98 μmol) and TEA (43.10 mg, 425.95 μmol) were added into the mixture. The mixture was stirred at 25° C. for another 2 hr and then concentrated under vacuum to give a residue. The residue was diluted with water (20.0 mL) and extracted with EtOAc (15.0 mL*2). The combined organic extracts were washed with brine (5.0 mL*2), dried over Na.sub.2SO.sub.4, filtered and concentrated under reduced pressure to give a residue. The residue was purified by prep-HPLC (TFA condition; column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 30%-53%, 7 min) and lyophilized to give compound 5 as a white solid.

(455) .sup.1H NMR (400 MHz, DMSO-d6) δ 12.52 (s, 1H), 9.31-9.29 (m, 1H), 8.37-8.36 (m, 1H), 8.26-8.23 (m, 2H), 7.94 (d, J=8.0 Hz, 1H), 7.82-7.76 (m, 3H), 7.61 (s, 1H), 7.39-7.36 (m, 1H), 7.21 (d, J=7.6 Hz, 1H), 4.29 (d, J=5.6 Hz, 2H), 4.23 (d, J=5.6 Hz, 2H), 2.65 (s, 6H), 1.88 (s, 3H) ppm.

(456) LCMS (ESI) m/z: [M+H].sup.+=516.3.

Example 51. Preparation of N-[2-[[4-[4-(aminomethyl)phenyl]thiazol-2-yl]amino]-2-oxo-ethyl]-3-(dimethylsulfamoyl)benzamide (Compound 22)

(457) ##STR00442##

Step 1: Preparation of 4-(2-aminothiazol-4-yl)benzonitrile (Intermediate i-51a)

(458) ##STR00443##

(459) To a mixture of 4-(2-bromoacetyl)benzonitrile (500 mg, 2.23 mmol, 79.78 μL) and NaF (0.1 g, 2.38 mmol) in H.sub.2O (8.0 mL) and MeOH (8.0 mL) was added thiourea (186.86 mg, 2.45 mmol). The mixture was stirred at 25° C. for 30 min and then diluted with water (10.0 mL) and extracted with EtOAc (35.0 mL*3). The combined organic extracts were washed with sat.NaHCO.sub.3 (20.0 mL*2), dried over Na.sub.2SO.sub.4, filtered and concentrated under reduced pressure to give intermediate i-51a as a yellow solid.

(460) .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ=7.97-7.95 (m, 2H), 7.82-7.80 (m, 2H), 7.32 (s, 1H), 7.19 (s, 2H) ppm.

Step 2: Preparation of tert-butyl N-[2-[[4-(4-cyanophenyl)thiazol-2-yl]amino]-2-oxo-ethyl]carbamate (Intermediate i-51b)

(461) ##STR00444##

(462) To a solution of 2-(tert-butoxycarbonylamino)acetic acid (287.26 mg, 1.64 mmol) in pyridine (6 mL) were added EDCl (1.43 g, 7.45 mmol) and intermediate i-51a (300.0 mg, 1.49 mmol), and the mixture was stirred at 25° C. for 48 hr. The reaction mixture was diluted with water (15.0 mL) and extracted with EtOAc (30.0 mL*3). The combined organic extracts were washed with brine (20.0 mL*2), dried over Na.sub.2SO.sub.4, filtered and concentrated under reduced pressure to give a residue. The residue was purified by Prep-HPLC (TFA condition; column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 38%-68%, 9 min) and lyophilized to give intermediate i-51b as a white solid.

(463) LCMS (ESI) m/z: [M+H].sup.+=359.2.

Step 3: Preparation of 2-amino-N-[4-(4-cyanophenyl)thiazol-2-yl]acetamide (Intermediate i-51c)

(464) ##STR00445##

(465) To a solution of intermediate i-51b (250 mg, 697.52 μmol) in DCM (2.0 mL) was added TFA (0.2 mL) and stirred at 25° C. for 4 hrs. The mixture was concentrated under reduced pressure to give intermediate i-51c (TFA salt) as a brown solid, which was used in the next step without further purification.

(466) LCMS (ESI) m/z: [M+H].sup.+=259.1.

Step 4: Preparation of N-[2-[[4-(4-cyanophenyl)thiazol-2-yl]amino]-2-oxo-ethyl]-3-(dimethylsulfamoyl)benzamide (Intermediate i-51d)

(467) ##STR00446##

(468) To a solution of 3-(dimethylsulfamoyl)benzoic acid (244.08 mg, 1.06 mmol) in DCM (5.0 mL) were added HATU (404.82 mg, 1.06 mmol), DIEA (500.36 mg, 3.87 mmol, 674.34 μL) and intermediate i-51c (250.0 mg, 967.87 μmol. The mixture was stirred at 25° C. for 24 hr. The reaction mixture was diluted with water (10.0 mL) and extracted with EtOAc (15.0 mL*3). The combined organic extracts were washed with brine (10.0 mL*2), dried over Na.sub.2SO.sub.4, filtered and concentrated under reduced pressure to give a residue. The residue was purified by prep-HPLC (TFA condition; column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 45%-75%, 9 min) and lyophilized to give intermediate i-51d as a white solid.

(469) 1H NMR (400 MHz, DMSO-d6) δ 12.27 (s, 1H), 9.33-9.30 (m, 1H), 8.26-8.23 (m, 2H), 8.08 (d, J=8.4 Hz, 2H), 7.95-7.89 (m, 4H), 7.82-7.78 (m, 1H), 4.24 (d, J=5.6 Hz, 2H), 2.65 (s, 6H) ppm.

(470) LCMS (ESI) m/z: [M+H].sup.+=469.2.

Step 5: Preparation of N-[2-[[4-[4-(aminomethyl)phenyl]thiazol-2-yl]amino]-2-oxo-ethyl]-3-(dimethylsulfamoyl)benzamide (compound 22)

(471) ##STR00447##

(472) To a solution of intermediate i-51d (200.0 mg, 425.95 μmol) in MeOH (1.0 mL) was added Raney-Ni (200.00 mg, 2.33 mmol) under N.sub.2. The mixture was stirred under H2 (50 psi) at 15° C. for 16 hours. The mixture was filtered and the filtrate was concentrated under vacuum. The residue was purified by prep-HPLC (Base column: Phenomenex Gemini 150*25 mm*10 μm; mobile phase: [water (0.05% ammonia hydroxide v/v)-ACN]; B %: 28%-58%, 11.5 min) and lyophilized to give compound 22 as a white solid.

(473) .sup.1H NMR (400 MHz, DMSO-d6) δ 9.16 (s, 1H), 8.26-8.23 (m, 2H), 7.93 (d, J=7.6 Hz, 1H), 7.82-7.77 (m, 3H), 7.43 (s, 1H), 7.35 (d, J=7.6, 2H), 4.15 (m, 2H), 3.72 (s, 2H), 2.65 (s, 6H) ppm. LCMS (ESI) m/z: [M+H].sup.+=474.3.

Example 52. Preparation of N-[2-[[4-[4-(acetamidomethyl)phenyl]thiazol-2-yl]amino]-2-oxo-ethyl]-3-(dimethylsulfamoyl)benzamide (Compound 11)

(474) ##STR00448##

(475) To a solution of N-[2-[[4-(4-cyanophenyl)thiazol-2-yl]amino]-2-oxo-ethyl]-3-(dimethylsulfamoyl)benzamide (100.00 mg, 212.98 μmol) in MeOH (2.0 mL) were added NiCl.sub.2.6H.sub.2O (126.56 mg, 532.44 μmol) and NaBH.sub.4 (80.57 mg, 2.13 mmol), the mixture was stirred at 25° C. for 2 hr, then Ac.sub.2O (21.74 mg, 212.98 μmol, 19.94 μL) and TEA (43.10 mg, 425.95 μmol, 59.29 μL) were added. The mixture was stirred at 25° C. for another 2 hr and then concentrated under vacuum to give a residue. The residue was diluted with water (20.0 mL) and extracted with EtOAc (15.0 mL*2). The combined organic extracts were washed with brine (5.0 mL*2), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure to give a residue. The residue was purified by prep-HPLC (TFA condition; column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 30%-53%, 7 min) and lyophilized to give compound 11 as a white solid.

(476) .sup.1H NMR (400 MHz, DMSO-d6) δ=12.49 (s, 1H), 9.32-9.29 (m, 1H), 8.38-8.35 (m, 1H), 8.27-8.23 (m, 2H), 7.95 (d, J=8.0 Hz, 1H), 7.86-7.79 (m, 3H), 7.60 (s, 1H), 7.31 (d, J=8.4 Hz, 2H), 4.27 (d, J=5.6 Hz, 2H), 4.23 (d, J=5.6 Hz, 2H), 2.66 (s, 6H), 1.88 (s, 3H) ppm.

(477) LCMS (ESI) m/z: [M+H].sup.+=516.3.

Example 53. Preparation of (S)—N-(6-acetamido-1-oxo-1-((4-phenylthiazol-2-yl)amino)hexan-2-yl)benzamide (Compound 43)

(478) ##STR00449##

Step 1: Preparation of (S)-6-((((9H-fluoren-9-yl)methoxy)carbonyl)amino)-2-((tert-butoxycarbonyl)amino)hexanoic acid (Intermediate i-53a)

(479) ##STR00450##

(480) To a solution of (2S)-6-amino-2-(tert-butoxycarbonylamino)hexanoic acid (1 g, 4.06 mmol) in THF (10.0 mL) was added a solution of NaHCO.sub.3 (682.14 mg, 8.12 mmol) in water (10.0 mL) and FmocOSu (1.51 g, 4.47 mmol). The reaction mixture was stirred at 20° C. for 3 hrs. The reaction mixture was poured into water (10.0 mL). The solution was adjusted to pH4 with saturated citric acid solution. The mixture was extracted with EtOAc (15 mL*3). The combined organic extracts were washed with brine (20.0 mL), dried over Na.sub.2SO.sub.4 and filtered. The filtrate was concentrated to give intermediate i-53a as a white solid. The compound was used directly in the next step.

(481) LCMS (ESI) m/z: [M+Na].sup.+=491.4

Step 2: Preparation of (S)-(9H-fluoren-9-yl)methyltert-butyl (6-oxo-6-((4-phenylthiazol-2-yl)amino)hexane-1,5-diyl)dicarbamate (Intermediate i-53b)

(482) ##STR00451##

(483) To a solution of intermediate i-53a (500 mg, 977.50 μmol) in DCM (5.0 mL) was added EEDQ (362.59 mg, 1.47 mmol) and 4-phenylthiazol-2-amine (172.27 mg, 977.50 μmol). The reaction mixture was stirred at 20° C. for 18 hr. The reaction mixture was poured into water (10.0 mL). The solution was extracted with EtOAc (10.0 mL*3). The combined organic extracts were washed with brine (20.0 mL), dried over Na.sub.2SO.sub.4 and filtered. The filtrate was concentrated to give a residue. The residue was purified by column chromatography (SiO.sub.2, Petroleum ether/Ethyl acetate=100:1 to 1:1) to give intermediate i-53b as a yellow oil.

(484) LCMS (ESI) m/z: [M+H].sup.+=627.5

Step 3: Preparation of (S)-(9H-fluoren-9-yl)methyl(5-amino-6-oxo-6-((4-phenylthiazol-2-yl)amino)hexyl)carbamate (Intermediate i-53c)

(485) ##STR00452##

(486) To a solution of intermediate i-53b (440 mg, 586.68 μmol) in DCM (5.0 mL) was added TFA (417.85 μL). The reaction mixture was stirred at 20° C. for 2 hrs. The reaction mixture was adjusted to pH 8 with saturated NaHCO.sub.3 solution. The solution was extracted with EtOAc (5.0 mL*3). The combined organic extracts were washed with brine (10.0 mL), dried over Na.sub.2SO.sub.4 and filtered. The filtrate was concentrated to give a residue. The residue was triturated with MTBE (10.0 mL). The solution was filtered. The filter cake was dried in vacuum to give intermediate i-53c as a white solid.

(487) LCMS (ESI) m/z: [M+Na].sup.+=549.4.

(488) Chiral HPLC: AS-3-MeOH (DEA)-5-40-3ML-35T, 2.386 min.

Step 4: Preparation of (S)-(9H-fluoren-9-yl)methyl(5-benzamido-6-oxo-6-((4-phenylthiazol-2-yl)amino)hexyl)carbamate (Intermediate i-53d)

(489) ##STR00453##

(490) To a solution of benzoic acid (41.68 mg, 341.33 μmol, 52.10 μL) in DCM (2.0 mL) was added HATU (142.76 mg, 375.46 μmol), DIEA (176.46 mg, 1.37 mmol) and intermediate i-53c (190 mg, 341.33 μmol). The reaction mixture was stirred at 20° C. for 3 hr. The reaction mixture was poured into water (2.0 mL). The solution was extracted with EtOAc (2.0 mL*3). The combined organic extracts were washed with brine (5.0 mL), dried over Na.sub.2SO.sub.4 and filtered. The filtrate was concentrated to give a residue. The residue was purified by flash silica gel chromatography (PE:EA=50:1-10:1-3:1-1:1) to give intermediate i-53d as a white solid.

(491) LCMS (ESI) m/z: [M+H].sup.+=631.4.

(492) Chiral HPLC: OJ-3-MeOH(DEA)-60-7MIN-3ML-35T, 3.881 min.

Step 5: Preparation of (S)—N-(6-amino-1-oxo-1-((4-phenylthiazol-2-yl)amino)hexan-2-yl)benzamide (Intermediate i-53e)

(493) ##STR00454##

(494) To a solution of intermediate i-53d (120 mg, 179.50 μmol) in DCM (1.2 mL) was added piperidine (120.00 μL). The solution was stirred at 20° C. for 2 hr. The mixture was poured into water (2.0 mL). The reaction mixture was diluted with saturated NaHCO.sub.3 solution (5 mL) and extracted with EtOAc (2.0 mL*3). The combined organic extracts were washed with brine (5.0 mL), dried over Na.sub.2SO.sub.4 and filtered. The filtrate was concentrated to give a residue. The residue was purified by prep-HPLC (column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.225% FA)-ACN]; B %: 17%-37%, 7 min) to give intermediate i-53e (FA salt) as a white solid.

(495) 1H NMR (400 MHz, DMSO-d6) δ 8.73 (d, J=6.8 Hz, 1H), 8.42 (s, 1H), 7.93-7.89 (m, 4H), 7.62 (s, 1H), 7.58-7.54 (m, 1H), 7.50-7.47 (m, 2H), 7.44-7.40 (m, 2H), 7.33-7.30 (m, 1H), 4.67-4.63 (m, 1H), 2.73 (s, 2H), 1.85 (d, J=6.4 Hz, 2H), 1.53-1.40 (m, 4H) ppm.

(496) LCMS (ESI) m/z: [M+H].sup.+=409.3.

(497) Chiral HPLC: OD-RH_0-60_1ML.M, 8.037 min.

Step 6: Preparation of (S)—N-(6-acetamido-1-oxo-1-((4-phenylthiazol-2-yl)amino)hexan-2-yl)benzamide (Compound 43)

(498) ##STR00455##

(499) A solution of intermediate i-53e (13 mg, 31.82 μmol) in Ac.sub.2O (0.2 mL) was stirred at 30° C. for 2 hr. The reaction mixture was filtered to give a filter cake. The filter cake was washed with MTBE (2.0 mL) and dried in vacuo to give a solid. The solid was poured into water (2.0 mL) and the solution was lyophilized to give compound 43 as a white solid.

(500) .sup.1H NMR (400 MHz, DMSO-d6) δ 12.46 (br s, 1H), 8.67 (d, J=7.2 Hz, 1H), 7.94-7.90 (m, 4H), 7.84 (s, 1H), 7.64 (s, 1H), 7.57-7.51 (m, 1H), 7.53-7.47 (m, 2H), 7.49-7.44 (m, 2H), 7.44-7.42 (m, 1H), 4.67-4.62 (m, 1H), 3.04 (m, 2H), 1.85 (m, 2H), 1.78 (s, 3H), 1.44-1.35 (m, 4H) ppm.

(501) LCMS (ESI) m/z: [M+H].sup.+=451.3.

(502) Chiral HPLC: Cellucoat-MeOH(DEA)-40-3ML-35T, 0.767 min.

Example 54. Preparation of 3-(isopropylsulfonyl)-N-(2-oxo-2-((4-phenylthiazol-2-yl)amino)ethyl)benzamide (Compound 6)

(503) ##STR00456##

Step 1: Preparation of methyl 3-(isopropylthio)benzoate (Intermediate i-54a)

(504) ##STR00457##

(505) To a solution of methyl 3-sulfanylbenzoate (200.0 mg, 1.19 mmol) in THF (2.0 mL) was added 2-iodopropane (303.17 mg, 1.78 mmol). Then NaH (57.07 mg, 1.43 mmol, 60% purity) was added in small portions over 5 min. The reaction mixture was stirred at 20° C. for 1 hr and then poured into 1N HCl (6 mL). The solution was extracted with EtOAc (5.0 mL*3). The combined organic extracts were washed with brine (10.0 mL), dried over Na.sub.2SO.sub.4 and filtered. The filtrate was concentrated to give a residue. The residue was purified by column chromatography (SiO.sub.2, PE/EA=100/1-1/1) to give intermediate i-54a as yellow oil.

(506) LCMS (ESI) m/z: [M+H].sup.+=211.3.

Step 2: Preparation of methyl 3-(isopropylsulfonyl)benzoate (Intermediate i-54b)

(507) ##STR00458##

(508) To a solution of intermediate i-54a (200.0 mg, 883.15 μmol) in DCM (2.0 mL) was added m-CPBA (320.04 mg, 1.58 mmol, 85% purity). The mixture was stirred at 20° C. for 1 hr and then poured into saturated a.q NaHCO.sub.3 (30.0 mL). The solution was extracted with EtOAc (20.0 mL*3). The combined organic extracts were washed with brine (50.0 mL), dried over Na.sub.2SO.sub.4 and filtered. The filtrate was concentrated to give intermediate i-54b as a white solid, which was used directly in the next step.

(509) LCMS (ESI) m/z: [M+H].sup.+=243.3.

Step 3: Preparation of 3-(isopropylsulfonyl)benzoic acid (Intermediate i-54c)

(510) ##STR00459##

(511) To a solution of intermediate i-54b (300.0 mg, 1.24 mmol) in dioxane (3.0 mL) and water (2.0 mL) was added LiOH (59.30 mg, 2.48 mmol). The reaction mixture was stirred at 20° C. for 1 hr and then adjusted pH 6 with saturated citric acid solution. Then the solution was extracted with EtOAc (10.0 mL*3). The combined organic extracts were washed with brine (20.0 mL), dried over Na.sub.2SO.sub.4 and filtered. The filtrate was concentrated to give a residue. The residue was purified with column chromatography (SiO.sub.2, PE:EA=50:1-20:1-10:1-3:1) to give intermediate i-54c as a white solid.

(512) .sup.1H NMR (400 MHz, DMSO-d6) δ 13.58 (s, 1H), 8.31-8.27 (m, 2H), 8.11-8.03 (m, 1H), 7.82 (d, J=7.6 Hz, 1H), 3.53-3.45 (m, 1H), 1.16 (d, J=6.8 Hz, 6H) ppm.

(513) LCMS (ESI) m/z: [M+H].sup.+=229.2.

Step 4: Preparation of 3-(isopropylsulfonyl)-N-(2-oxo-2-((4-phenylthiazol-2-yl)amino)ethyl)benzamide (Compound 6)

(514) ##STR00460##

(515) To a solution of intermediate i-54c (30.0 mg, 129.42 μmol) in DCM (0.3 mL) were added HATU (59.05 mg, 155.30 μmol), DIEA (66.90 mg, 517.66 μmol) and 2-amino-N-(4-phenylthiazol-2-yl) acetamide (30.19 mg, 86.93 μmol). The reaction mixture was stirred at 30° C. for 12 hr and then poured into water (2.0 mL). The solution was extracted with EtOAc (2.0 mL*3). The combined organic extracts were washed with brine (5.0 mL), dried over Na.sub.2SO.sub.4 and filtered. The filtrate was concentrated to give a residue. To the residue was added MeOH (0.5 mL) and stirred for 5 min, then filtered. The solid was lyophilized to give compound 6 as a white solid.

(516) .sup.1H NMR (400 MHz, DMSO-d6) δ 12.49 (s, 1H), 9.31-9.28 (m, 1H), 8.38 (s, 1H), 8.27 (d, J=8.0 Hz, 1H), 8.05 (d, J=7.6 Hz, 1H), 7.90 (d, J=7.2 Hz, 2H), 7.84-7.80 (m, 1H), 7.65 (s, 1H), 7.45-7.43 (m, 2H), 7.41-7.32 (m, 1H), 4.24 (d, J=5.6 Hz, 2H), 3.54-3.47 (m, 1H), 1.18 (d, J=6.8 Hz, 6H) ppm.

(517) LCMS (ESI) m/z: [M+H].sup.+=444.3.

Example 55. Preparation of 3-(dimethylsulfamoyl)-N-[2-oxo-2-[(6-phenyl-2-pyridyl)amino]ethyl]benzamide (Compound 41)

(518) ##STR00461##

Step 1: Preparation of tert-butyl N-[2-oxo-2-[(6-phenyl-2-pyridyl)amino]ethyl]carbamate (Intermediate i-55a)

(519) ##STR00462##

(520) To a solution of 2-(tert-butoxycarbonylamino)acetic acid (113.21 mg, 646.26 μmol), DIPEA (303.73 mg, 2.35 mmol) and HATU (245.73 mg, 646.26 μmol) in DCM (2.0 mL) was added 6-phenylpyridin-2-amine (100.0 mg, 587.51 μmol), and the mixture was stirred at 20° C. for 2 hr. The reaction mixture was concentrated under reduced pressure to give a residue. The residue was purified by column chromatography (SiO.sub.2, Petroleum ether/Ethyl acetate=5:1 to 2:1) to give intermediate i-55a as a light yellow oil.

(521) .sup.1H NMR (400 MHz, CDCl.sub.3) δ 8.48 (s, 1H), 8.14 (d, J=8 Hz, 1H), 7.96 (d, J=7.2 Hz, 2H), 7.82-7.78 (m, 1H), 7.52-7.45 (m, 4H), 5.22-5.21 (m, 1H), 4.03 (s, 2H), 1.52 (s, 9H) ppm.

(522) LCMS (ESI) m/z: [M+H].sup.+=328.0.

Step 2: Preparation of 2-amino-N-(6-phenyl-2-pyridyl)acetamide (Intermediate i-55b)

(523) ##STR00463##

(524) To a solution of Intermediate i-55a (130 mg, 381.21 μmol) in DCM (2.0 mL) was added TFA (192.00 μL) and the mixture was stirred at 20° C. for 12 hr. The reaction mixture was concentrated under reduced pressure to give a residue. The residue was triturated with MTBE (5 mL), the solid was collected by filtration and dried in vacuo to give Intermediate i-55b (TFA salt) as a white solid.

(525) LCMS (ESI) m/z: [M+H].sup.+=228.0.

Step 3: Preparation of 3-(dimethylsulfamoyl)-N-[2-oxo-2-[(6-phenyl-2-pyridyl)amino]ethyl]benzamide (Compound 41) trifluoroacetate salt

(526) ##STR00464##

(527) To a solution of 3-(dimethylsulfamoyl)benzoic acid (36.58 mg, 159.54 μmol), HATU (60.66 mg, 159.54 μmol) and DIPEA (74.98 mg, 580.16 μmol) in DCM (1.0 mL) was added Intermediate i-55b (50.0 mg, 145.04 μmol) and the mixture was stirred at 25° C. for 2 hr. The reaction mixture was concentrated under reduced pressure to give a residue. The residue was purified by prep-HPLC (column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 35%-65%, 9 min) and lyophilized to give compound 41 (TFA salt) as a white solid.

(528) .sup.1H NMR (400 MHz, DMSO-d6) δ 10.68 (s, 1H), 9.24-9.21 (m, 1H), 8.27-8.26 (m, 2H), 8.10-8.08 (m, 2H), 8.02 (d, J=8.0 Hz, 1H), 7.96-7.93 (m, 1H), 7.90-7.88 (m, 1H), 7.82-7.78 (m, 1H), 7.69 (d, J=8.0 Hz, 1H), 7.51-7.46 (m, 3H), 4.23 (d, J=6.0 Hz, 2H), 2.68-2.66 (m, 6H) ppm.

(529) LCMS (ESI) m/z: [M+H].sup.+=439.0.

Example 56. Preparation of N-[(1S)-3-methylsulfanyl-1-[(4-phenylthiazol-2-yl)carbamoyl]propyl]thiophene-2-carboxamide (Compound 58)

(530) ##STR00465##

(531) To a solution of thiophene-2-carboxylic acid (22.93 mg, 178.90 μmol), HATU (68.02 mg, 178.90 μmol) and DIPEA (84.08 mg, 650.55 μmol) in DCM (1.0 mL) was added (2S)-2-amino-4-methylsulfanyl-N-(4-phenylthiazol-2-yl)butanamide (50.00 mg, 162.64 μmol) and the mixture was stirred at 20° C. for 2 hr. The reaction mixture was concentrated under reduced pressure to give a residue. The residue was purified by prep-HPLC (column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 50%-80%, 9 min) and lyophilized to give compound 58 as a white solid.

(532) .sup.1H NMR (400 MHz, CDCl.sub.3) δ=7.69 (d, J=7.2 Hz, 2H), 7.62-7.61 (m, 1H), 7.48 (d, J=4.8 Hz, 1H), 7.40-7.36 (m, 2H), 7.34-7.30 (m, 1H), 7.26-7.24 (d, J=8.0 Hz, 1H), 7.07 (s, 1H), 7.06-7.03 (m, 1H), 5.00-4.95 (m, 1H), 2.68-2.63 (m, 2H), 2.29-2.20 (m, 2H), 2.10 (s, 3H) ppm.

(533) LCMS (ESI) m/z: [M+H].sup.+=418.0

(534) Chiral HPLC: Amycoat-MeOH(DEA)-40-7 min-3 mL-35T, 3.772 min.

Example 57. Preparation of (S)—N-(4-(methylthio)-1-oxo-1-((4-phenylthiazol-2-yl)amino)butan-2-yl)benzo[b]thiophene-2-carboxamide (Compound 71)

(535) ##STR00466##

(536) To a solution of benzothiophene-2-carboxylic acid (50.0 mg, 280.57 μmol, 47.17 μL) in DCM (1.0 mL) were added HATU (128.02 mg, 336.69 μmol), DIEA (108.79 mg, 841.71 μmol) and (2S)-2-amino-4-methylsulfanyl-N-(4-phenylthiazol-2-yl)butanamide (118.25 mg, 280.57 μmol). The reaction mixture was stirred at 20° C. for 12 hr. Then the reaction mixture was poured into water (2.0 mL), extracted with EtOAc (2.0 mL*3). The combined organic extracts were washed with brine (5.0 mL), dried over Na.sub.2SO.sub.4, filtered and concentrated under vacuum to give a residue. The residue was purified by prep-HPLC (column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 52%-82%, 9 min) and lyophilized to give compound 71 as a white solid.

(537) .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ=12.58 (s, 1H), 9.08 (d, J=7.2 Hz, 1H), 8.31 (s, 1H), 8.03-7.92 (m, 2H), 7.91 (d, J=7.2 Hz, 2H), 7.66 (s, 1H), 7.48-7.43 (m, 4H), 7.41-7.33 (m, 1H), 4.79-4.74 (m, 1H), 2.68-2.66 (m, 1H), 2.59-2.54 (m, 1H), 2.16-2.11 (m, 5H) ppm.

(538) LCMS (ESI) m/z: [M+H].sup.+=468.3.

(539) Chiral HPLC: Cellucoat-MeOH+CAN(DEA)-40-7MIN-3ML-35T, 5.321 min.

Example 58. Preparation of 5-(dimethylsulfamoyl)-N-[(1S)-3-methylsulfanyl-1-[(4-phenylthiazol-2-yl)carbamoyl]propyl]thiophene-2-carboxamide (Compound 62)

(540) ##STR00467##

Step 1: Preparation of 4-chlorosulfonylthiophene-2-carboxylic acid (intermediate i-58a) and 5-chlorosulfonylthiophene-2-carboxylic acid (Intermediate i-58b)

(541) ##STR00468##

(542) Thiophene-2-carboxylic acid (300.0 mg, 2.34 mmol) was added to sulfurochloridic acid (1.36 g, 11.71 mmol) and the mixture was stirred at 100° C. for 1 hr. The reaction mixture was carefully added dropwise to ice water (30.0 mL) and stirred for 5 minutes. The precipitate was filtered and dried in vacuo to give a mixture of i-58a and i-58b as a white solid, which was used in the next step without further purification.

(543) .sup.1H NMR (400 MHz, DMSO-d6) δ 7.81 (d, J=1.6 Hz, 2H), 7.60 (d, J=1.6 Hz, 2H), 7.51 (d, J=4 Hz, 1H), 7.13 (d, J=4 Hz, 1H) ppm.

Step 2: Preparation of 4-(dimethylsulfamoyl)thiophene-2-carboxylicacid (Intermediate i-58c) and 5-(dimethylsulfamoyl)thiophene-2-carboxylic acid (Intermediate i-58d)

(544) ##STR00469##

(545) To a mixture of i-58a and i-58b (260.0 mg, crude) in THF (2.0 mL) was added N-methylmethanamine (2.29 mL, 4.59 mmol, 2.0 M in THF) at 0° C., the mixture was stirred at 25° C. for 2 hr. The reaction mixture was concentrated under reduced pressure to give a residue. The residue was purified by prep-HPLC (column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 18%-40%, 12 min) and lyophilized to give intermediate i-58c as a white solid and intermediate i-58d as a white solid.

(546) Intermediate i-58c:

(547) .sup.1H NMR (400 MHz, DMSO-d6) δ 8.51 (d, J=1.6 Hz, 1H), 7.78 (d, J=1.6 Hz, 1H), 2.73-2.66 (m, 7H) ppm.

(548) LCMS (ESI) m/z: [M+H].sup.+=236.0.

(549) Intermediate i-58d:

(550) .sup.1H NMR (400 MHz, DMSO-d6) δ 7.82 (d, J=4.0 Hz, 1H), 7.66 (d, J=4.0 Hz, 1H), 2.70-2.67 (m, 6H) ppm.

(551) LCMS (ESI) m/z: [M+H].sup.+=236.0.

Step 3: Preparation of 5-(dimethylsulfamoyl)-N-[(1S)-3-methylsulfanyl-1-[(4-phenylthiazol-2-yl)carbamoyl]propyl]thiophene-2-carboxamide (Compound 62)

(552) ##STR00470##

(553) To a solution of intermediate i-58d (40.0 mg, 170.01 μmol), DIPEA (87.89 mg, 680.04 μmol) and HATU (71.11 mg, 187.01 μmol) in DCM (1.0 mL) was added (2S)-2-amino-4-methylsulfanyl-N-(4-phenylthiazol-2-yl)butanamide (78.82 mg, 187.01 μmol) and the mixture was stirred at 30° C. for 2 hr. The reaction mixture was concentrated under reduced pressure to give a residue. The residue was purified by prep-HPLC (column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 43%-73%, 10 min) and lyophilized to give compound 62 as a white solid.

(554) .sup.1H NMR (400 MHz, DMSO-d6) δ=12.61 (s, 1H), 9.15 (d, J=7.6 Hz, 1H), 8.07 (d, J=4.0 Hz, 1H), 7.92-7.90 (m, 2H), 7.71 (d, J=4.0 Hz, 1H), 7.66 (s, 1H), 7.46-7.42 (m, 2H), 7.35-7.31 (m, 1H), 4.78-4.67 (m, 1H), 2.69 (s, 6H), 2.65-2.56 (m, 2H), 2.17-2.07 (m, 5H) ppm.

(555) LCMS (ESI) m/z: [M+H].sup.+=525.1.

Example 59. Preparation of N-(2-((4-(2,3-dihydrobenzo[b][1,4]dioxin-6-yl)thiazol-2-yl)amino)-2-oxoethyl)-3-(N,N-dimethylsulfamoyl)benzamide (Compound 15)

(556) ##STR00471##

(557) To a solution of N-[2-[(4-bromothiazol-2-yl)amino]-2-oxo-ethyl]-3-(dimethylsulfamoyl)benzamide (30.0 mg, 67.07 μmol) and 2,3-dihydro-1,4-benzodioxin-6-ylboronic acid (36.21 mg, 201.20 μmol) in dioxane (2.0 mL) and H.sub.2O (0.2 mL) were added DIEA (43.34 mg, 335.33 μmol) and palladium; tritert-butylphosphane (17.14 mg, 33.53 μmol) under N.sub.2. The mixture was stirred at 100 C for 2 hr. The reaction mixture was filtered. The filtrate was concentrated to give a residue. The residue was purified by Prep-HPLC (column: Boston pH-lex 150*25 10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 39%-59%, 8 min) and lyophilized to give compound 15 as a white solid.

(558) 1H NMR (400 MHz, DMSO-d.sub.6) δ=12.42 (s, 1H), 9.30-9.28 (m, 1H), 8.26-8.22 (m, 2H), 7.93 (d, J=7.6 Hz, 1H), 7.82-7.80 (m, 1H), 7.48 (s, 1H), 7.39-7.35 (m, 2H), 6.89 (d, J=8.0 Hz, 1H), 4.27 (s, 4H), 4.22 (d, J=6.0 Hz, 2H), 2.65 (s, 6H) ppm.

(559) LCMS (ESI) m/z: [M+H].sup.+=503.3.

Example 60. Preparation of (S)-3-(isopropylsulfonyl)-N-(4-(methylthio)-1-oxo-1-((4-phenylthiazol-2-yl)amino)butan-2-yl)benzamide (Compound 14)

(560) ##STR00472##

(561) To a solution of 3-(isopropylsulfonyl)benzoic acid (30.0 mg, 129.42 μmol) in DCM (0.3 mL) were added HATU (59.05 mg, 155.30 μmol), DIEA (66.90 mg, 517.66 μmol) and (2S)-2-amino-4-methylsulfanyl-N-(4-phenylthiazol-2-yl)butanamide (54.54 mg, 129.42 μmol). The reaction mixture was stirred at 30° C. for 12 hr. Then the reaction mixture was poured into water (2.0 mL), extracted with EtOAc (2.0 mL*3). The combined organic extracts were washed with brine (5.0 mL), dried over Na.sub.2SO.sub.4 and filtered. The filtrate was concentrated to give a residue. The residue was purified by Pre-HPLC (column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 45%-75%, 9 min) and lyophilized to give compound 14 as a white solid.

(562) 1H NMR (400 MHz, DMSO-d.sub.6) δ=12.59 (s, 1H), 9.12 (d, J=7.2 Hz, 1H), 8.39 (s, 1H), 8.30 (d, J=8.0 Hz, 1H), 8.05 (d, J=7.6 Hz, 1H), 7.91 (d, J=7.2 Hz, 2H), 7.83-7.79 (m, 1H), 7.66 (s, 1H), 7.45-7.41 (m, 2H), 7.34-7.30 (m, 1H), 4.82-4.77 (m, 1H), 3.51 (s, 1H), 2.64-2.55 (m, 2H), 2.16-2.09 (m, 2H), 2.10 (s, 3H), 1.18-1.16 (m, 6H) ppm.

(563) LCMS (ESI) m/z: [M+H].sup.+=518.3

(564) Chiral HPLC: Cellucoat-MeOH(DEA)-40-3ML-35T, 1.119 min.

Example 61 Preparation of N-[(1S)-3-methylsulfanyl-1-[(4-phenylthiazol-2-yl)carbamoyl]propyl]-3-(trifluoromethyl)benzamide (Compound 77)

(565) ##STR00473##

(566) To a solution of 3-(trifluoromethyl)benzoic acid (22.55 mg, 118.64 μmol) in DCM (3 mL) was added DIPEA (46.00 mg, 355.91 μmol) and HATU (49.62 mg, 130.50 μmol). The mixture was stirred at 30° C. for 10 min. (2S)-2-amino-4-methylsulfanyl-N-(4-phenylthiazol-2-yl)butanamide (50 mg, 118.64 μmol) was added and the reaction mixture was stirred at 30° C. for 2 hr. The reaction mixture was concentrated to dryness to give a residue. The residue was purified by prep-HPLC (column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 53%-80%, 12 min) and lyophilized to afford compound 77 (97.3% ee value) as a white solid.

(567) .sup.1H NMR (400 MHz, METHANOL-d4) δ 8.27 (m, 1H), 8.20 (d, J=8.0 Hz, 1H), 7.93-7.89 (m, 3H), 7.73 (m, 1H), 7.42-7.39 (m, 3H), 7.34-7.28 (m, 1H), 4.97-4.95 (m, 1H), 2.73-2.66 (m, 2H), 2.30-2.24 (m, 2H), 2.16 (s, 3H) ppm.

(568) LCMS (ESI) m/z: [M+H].sup.+=480.1.

(569) Chiral SFC: OD-3_5CM_MEOH(DEA)_40_3ML_5MIN_T35.M., 0.601 min.

Example 62. Preparation of N-[(1S)-3-methylsulfanyl-1-[(4-phenylthiazol-2-yl)carbamoyl]propyl]-4-(trifluoromethyl)benzamide (Compound 68)

(570) ##STR00474##

(571) To a solution of 4-(trifluoromethyl)benzoic acid (22.55 mg, 118.64 μmol) in DCM (3 mL) was added DIPEA (46.00 mg, 355.91 μmol) and HATU (49.62 mg, 130.50 μmol). The mixture was stirred at 30° C. for 10 min. (2S)-2-amino-4-methylsulfanyl-N-(4-phenylthiazol-2-yl)butanamide (50 mg, 118.64 μmol) was added and the reaction mixture was stirred at 30° C. for 2 hr. The reaction mixture was concentrated to dryness to give a residue. The residue was purified by prep-HPLC (column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 65%-85%, 8 min) and lyophilized to afford compound 68 as a white solid.

(572) .sup.1HNMR (400 MHz, METHANOL-d4) δ 8.09 (d, J=8.0 Hz, 1H), 7.92 (d, J=7.2 Hz, 1H), 7.83 (d, J=8.4 Hz, 2H), 7.42-7.39 (m, 3H), 7.33-7.31 (m, 1H), 4.97-4.94 (m, 1H), 2.73-2.66 (m, 2H), 2.33-2.24 (m, 2H), 2.16 (s, 3H) ppm.

(573) LCMS (ESI) m/z: [M+H].sup.+=480.1.

(574) Chiral SFC: Cellucoat-MeOH(DEA)-40-7 min-3 mL-35T, 0.937 min.

Example 63. Preparation of N4-methyl-N1-[(1S)-3-methylsulfanyl-1-[(4-phenylthiazol-2-yl)carbamoyl]propyl]terephthalamide (Compound 72)

(575) ##STR00475##

Step 1: Preparation of methyl 4-[[(1S)-3-methylsulfanyl-1-[(4-phenylthiazol-2-yl)carbamoyl]propyl]carbamoyl]benzoate (Intermediate i-63a)

(576) ##STR00476##

(577) To a solution of 4-methoxycarbonylbenzoic acid (85.49 mg, 474.54 μmol) in DCM (5 mL) were added DIPEA (183.99 mg, 1.42 mmol) and HATU (198.48 mg, 522.00 μmol). The mixture was stirred at 30° C. for 10 min. (2S)-2-amino-4-methylsulfanyl-N-(4-phenylthiazol-2-yl)butanamide (200 mg, 474.54 μmol) was added and the reaction mixture was stirred at 30° C. for 2 hr. The reaction mixture was quenched by addition water (10 mL) and extracted with ethyl acetate (10 mL×2). The combined organic extracts were washed with water (10 mL), dried over Na.sub.2SO.sub.4, filtered and concentrated under reduced pressure to give intermediate i-63a as colorless oil, which was used in next step without further purification.

(578) LCMS (ESI) m/z: [M+H].sup.+=470.3.

Step 2: Preparation of 4-[[(1S)-3-methylsulfanyl-1-[(4-phenylthiazol-2-yl) carbamoyl]propyl]carbamoyl]benzoic acid (Intermediate i-63b)

(579) ##STR00477##

(580) To a solution of intermediate i-63a (258.82 mg, 468.50 μmol) in MeOH (2.0 mL) and H.sub.2O (2.0 mL) was added NaOH (36.39 mg, 909.75 μmol). The mixture was stirred at 30° C. for 18 hr. The reaction mixture was concentrated in vacuo. The pH of the residue was adjusted to pH 4 with 20% HCl solution. A white precipitate was formed and the mixture was filtered. The filtration cake was washed with water (4.0 mL) and concentrated to dryness to give intermediate i-63b as an off-white solid.

(581) LCMS (ESI) m/z: [M+H].sup.+=456.3.

Step 3: Preparation of N4-methyl-N1-[(1S)-3-methylsulfanyl-1-[(4-phenylthiazol-2-yl)carbamoyl]propyl]terephthalamide (Compound 72)

(582) ##STR00478##

(583) To a solution of intermediate i-63b (40 mg, 81.66 μmol) in DCM (3 mL) was added HATU (36.15 mg, 95.07 μmol) and DIPEA (20.48 mg, 158.46 μmol). The mixture was stirred at 30° C. for 10 min. methanamine (2 M, 396.05 μL) was added and the reaction mixture was stirred at 30° C. for 3 hr. The reaction mixture was concentrated to dryness to give a residue. The residue was purified by prep-HPLC (column: Boston pH-lex 150*25 10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 50%-70%, 8 min) and lyophilized to afford compound 72 as a white solid.

(584) .sup.1H NMR (400 MHz, METHANOL-d4) δ 8.00 (d, J=8.4 Hz, 2H), 7.94-7.91 (m, 4H), 7.42-7.39 (m, 3H), 7.33-7.29 (m, 1H), 4.97-4.94 (m, 1H), 2.95 (s, 3H), 2.73-2.66 (m, 2H), 2.33-2.24 (m, 2H), 2.16 (s, 3H) ppm.

(585) LCMS (ESI) m/z: [M+H]+=469.3.

Example 64. Preparation of (S)-tert-butyl 3-((4-(methylthio)-1-oxo-1-((4-phenylthiazol-2-yl)amino)butan-2-yl)carbamoyl)benzylcarbamate (Compound 75)

(586) ##STR00479##

Step 1: Preparation of (S)-tert-butyl 3-((4-(methylthio)-1-oxo-1-((4-phenylthiazol-2-yl)amino)butan-2-yl)carbamoyl)benzylcarbamate (Intermediate i-64a)

(587) ##STR00480##

(588) To a solution of 3-[(tert-butoxycarbonylamino)methyl]benzoic acid (29.81 mg, 118.64 μmol) in DCM (2 mL) were added HATU (67.66 mg, 177.96 μmol) and DIPEA (61.33 mg, 474.56 μmol), then (2S)-2-amino-4-methylsulfanyl-N-(4-phenylthiazol-2-yl)butanamide (50 mg, 118.64 μmol) was added. The mixture was stirred at 10° C. for 12 hr. The reaction mixture was concentrated under reduced pressure. The residue was purified by prep-TLC (SiO2, PE:EA=1:1) to give intermediate i-64a as a white solid.

(589) LCMS (ESI) m/z: [M+H].sup.+=541.1.

Step 2: Preparation of (S)-tert-butyl 3-((4-(methylthio)-1-oxo-1-((4-phenylthiazol-2-yl)amino)butan-2-yl)carbamoyl)benzylcarbamate (Compound 75) trifluoroacetate salt

(590) ##STR00481##

(591) To a solution of intermediate i-64a (50 mg, 76.37 μmol) in DCM (2.0 mL) was added TFA (1.0 mL). The mixture was stirred at 12° C. for 2 hr. The reaction mixture was concentrated under reduced pressure to remove DCM. The residue was purified by prep-HPLC (TFA condition; column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 25%-55%, 9 min) and lyophilized to give compound 75 (TFA salt) as a white solid.

(592) 1H NMR (400 MHz, METHANOL-d4) δ=7.99-7.97 (m, 2H), 7.91-7.89 (m, 2H), 7.66-7.64 (m, 1H), 7.61-7.59 (m, 1H), 7.41-7.36 (m, 3H), 7.31-7.29 (m, 1H), 4.95-4.91 (m, 1H), 4.19 (s, 2H), 2.72-2.65 (m, 2H), 2.30-2.20 (m, 2H), 2.15 (s, 3H) ppm.

(593) LCMS (ESI) m/z: [M+H].sup.+=441.0.

Example 65. Preparation of 4-(aminomethyl)-N-[3-methylsulfanyl-1-[(4-phenylthiazol-2-yl)carbamoyl]propyl]benzamide (Compound 57)

(594) ##STR00482##

Step 1: Preparation of tert-butyl N-[[4-[[(1S)-3-methylsulfanyl-1-[(4-phenylthiazol-2-yl)carbamoyl]propyl]carbamoyl]phenyl]methyl]carbamate (Intermediate i-65a)

(595) ##STR00483##

(596) To a mixture of 4-[(tert-butoxycarbonylamino)methyl]benzoic acid (89.43 mg, 355.91 μmol) in DCM (1 mL) was added HATU (202.99 mg, 533.86 μmol) and DIPEA (247.97 μL, 1.42 mmol). The mixture was stirred at 30° C. for 15 min, then (2S)-2-amino-4-methylsulfanyl-N-(4-phenylthiazol-2-yl)butanamide (150 mg, 355.91 μmol) was added and stirred at 30° C. for 16 hours. The reaction mixture was evaporated to dryness to give the crude product. The residue was purified by prep-HPLC (column: Boston pH-lex 150*25 10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 59%-79%, 8 min) and lyophilized to give intermediate i-65a as a white solid.

(597) .sup.1H NMR (400 MHz, MeOD-d.sub.4) δ=7.91-7.86 (m, 4H), 7.41-7.37 (m, 5H), 7.31-7.28 (m, 1H), 4.92 (br d, J=5.2 Hz, 1H), 4.30 (s, 2H), 2.74-2.60 (m, 2H), 2.33-2.17 (m, 2H), 2.14 (s, 3H), 1.46 (s, 9H) ppm.

(598) LCMS (ESI) m/z: [M+H].sup.+=541.3.

Step 2: Preparation of 4-(aminomethyl)-N-[3-methylsulfanyl-1-[(4-phenylthiazol-2-yl)carbamoyl]propyl]benzamide trifluoroacetate salt (compound 57)

(599) ##STR00484##

(600) To a solution of intermediate i-65a (150.00 mg, 277.42 μmol) in DCM (1 mL) was added TFA (205.40 μL). The mixture was stirred at 30° C. for 2 hours. The reaction mixture was evaporated to dryness to give the crude product. The residue was purified by prep-HPLC (column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 18%-48%, 12 min) and lyophilized to give compound 57 (TFA salt) as a white solid.

(601) .sup.1H NMR (400 MHz, MeOD-d.sub.4) δ=8.00 (d, J=8.4 Hz, 2H), 7.91-7.89 (m, 2H), 7.58 (d, J=8.4 Hz, 2H), 7.41- 7.37 (m, 3H), 7.32-7.28 (m, 1H), 4.94-4.91 (m, 1H), 4.20 (s, 2H), 2.71-2.64 (m, 2H), 2.31-2.22 (m, 2H), 2.14 (s, 3H) ppm.

(602) LCMS (ESI) m/z: [M+H].sup.+=441.2.

Example 66. Preparation of 4-[(dimethylamino)methyl]-N-[(1S)-3-methylsulfanyl-1-[(4-phenylthiazol-2-yl)carbamoyl]propyl]benzamide (Compound 60)

(603) ##STR00485##

(604) To a mixture of 4-[(dimethylamino)methyl]benzoic acid;hydrochloride (25.59 mg, 118.64 μmol) in DCM (1 mL) was added HATU (67.66 mg, 177.96 μmol) and DIPEA (82.66 μL, 474.56 μmol). The mixture was stirred at 30° C. for 15 min, then (2S)-2-amino-4-methylsulfanyl-N-(4-phenylthiazol-2-yl)butanamide (50 mg, 118.64 μmol) was added and stirred at 30° C. for 2 hours. The reaction mixture was evaporated to dryness. The residue was purified by prep-HPLC (column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 25%-55%, 9 min) and lyophilized to give compound 60 as an off-white solid.

(605) .sup.1H NMR (400 MHz, MeOD-d.sub.4) δ=8.03 (d, J=8.0 Hz, 2H), 7.90 (d, J=8.0 Hz, 2H), 7.63 (d, J=8.4 Hz, 2H), 7.40-7.36 (m, 3H), 7.31-7.29 (m, 1H), 4.95-4.91 (m, 1H), 4.39 (s, 2H), 2.88 (s, 6H), 2.71-2.64 (m, 2H), 2.29-2.19 (m, 2H), 2.14 (s, 3H) ppm.

(606) LCMS (ESI) m/z: [M+H].sup.+=469.1.

Example 67. Preparation of 4-(acetamidomethyl)-N-[3-methylsulfanyl-1-[(4-phenylthiazol-2-yl)carbamoyl]propyl]benzamide (Compound 74)

(607) ##STR00486##

(608) To a solution of 4-(aminomethyl)-N-[3-methylsulfanyl-1-[(4-phenylthiazol-2-yl)carbamoyl]propyl]benzamide (50 mg, 90.15 μmol, TFA salt) in DCM (1 mL) was added TEA (62.74 μL, 450.77 μmol) followed by Ac.sub.2O (21.11 μL, 225.39 μmol) at 0° C. The mixture was stirred at 30° C. for 1 hour. The reaction mixture was evaporated to dryness. The residue was purified by prep-HPLC (column: Xtimate C18 150*25 mm*5 μm; mobile phase: [water (0.05% ammonia hydroxide v/v)-ACN]; B %: 30%-60%, 12 min) and lyophilized to give compound 74 as an off-white solid.

(609) .sup.1H NMR (400 MHz, MeOD-d.sub.4) δ=7.90-7.87 (m, 4H), 7.41-7.36 (m, 5H), 7.31-7.27 (m, 1H), 4.92-4.90 (m, 1H), 4.43 (s, 2H), 2.73-2.60 (m, 2H), 2.27-2.21 (m, 2H), 2.13 (s, 3H), 2.01 (s, 3H) ppm.

(610) LCMS (ESI) m/z: [M+H].sup.+=483.3.

Example 68. Preparation of Compound N-[(1S)-3-methylsulfanyl-1-[(4-phenylthiazol-2-yl)carbamoyl]propyl]-4-methylsulfonyl-benzamide (Compound 68)

(611) ##STR00487##

(612) To a solution of 4-methylsulfonylbenzoic acid (23.75 mg, 118.64 μmol) in DCM (3 mL) was added DIPEA (46.00 mg, 355.91 μmol) and HATU (49.62 mg, 130.50 μmol). The mixture was stirred at 30° C. for 10 min. (2S)-2-amino-4-methylsulfanyl-N-(4-phenylthiazol-2-yl)butanamide (50 mg, 118.64 μmol) was added and the reaction mixture was stirred at 30° C. for 2 hr. The reaction mixture was concentrated to dryness to give a residue. The residue was purified by prep-HPLC (column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 45%-69%, 12 min) and lyophilized to afford compound 68 as a white solid.

(613) 1H NMR (400 MHz, METHANOL-d4) δ=8.19-8.05 (m, 4H), 7.92 (d, J=7.2 Hz, 2H), 7.42-7.39 (m, 3H), 7.33-7.31 (m, 1H), 4.97-4.94 (m, 1H), 3.19 (s, 3H), 2.73-2.66 (m, 2H), 2.33-2.24 (m, 2H), 2.16 (s, 3H) ppm.

(614) LCMS (ESI) m/z: [M+H].sup.+=490.1.

Example 69. Preparation of 4-methoxy-N-[(1S)-3-methylsulfanyl-1-[(4-phenylthiazol-2-yl)carbamoyl]propyl]-3-methylsulfonyl-benzamide (Compound 20)

(615) ##STR00488##

(616) To a solution of 4-methoxy-3-methylsulfonyl-benzoic acid (21.85 mg, 94.91 μmol) in DCM (1 mL) was added HATU (43.30 mg, 113.89 μmol) and DIEA (36.80 mg, 284.73 μmol) and the mixture was stirred at 15° C. for 5 min. Then (2S)-2-amino-4-methylsulfanyl-N-(4-phenylthiazol-2-yl) butanamide (40 mg, 94.91 μmol) was added and the mixture was stirred at 15° C. for 1 hr. The solvent was removed under reduced pressure and the residue was purified by Prep.HPLC (column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 42%-72%, 9 min) and lyophilized to give compound 20 as a white solid.

(617) .sup.1H NMR (400 MHz, MeOD) δ=8.49 (d, J=2.4, 1H), 8.26-8.23 (m, 1H), 7.90-7.88 (m, 2H), 7.40-7.36 (m, 4H), 7.31-7.27 (m, 1H), 4.92-4.91 (m, 1H), 4.08 (s, 3H), 3.26 (s, 3H), 2.73-2.60 (m, 2H), 2.34-2.17 (m, 2H), 2.14 (s, 3H) ppm.

(618) LCMS (ESI) m/z: [M+H].sup.+=520.1.

Example 70. Preparation of 4-methyl-3-(methylsulfonyl)-N-(2-oxo-2-((4-phenylthiazol-2-yl)amino)ethyl)benzamide (Compound 19)

(619) ##STR00489##

Step 1: Preparation of 4-methyl-3-(methylsulfonyl)benzoic acid (Intermediate i-70a)

(620) ##STR00490##

(621) NaOH (13.14 mg, 328.57 μmol) was dissolved in H.sub.2O (1 mL) and then added into solution of methyl 4-methyl-3-methylsulfonyl-benzoate (50 mg, 219.04 μmol) in THF (0.5 mL) and MeOH (0.2 mL). The reaction mixture was stirred at 30° C. for 17 hr. The reaction mixture was diluted with water (3 mL) then washed by EtOAc (5 mL). The water layer was adjusted to pH=3 with 2 M aq. HCl, then extracted by EtOAc (5 mL*3). The combined organic extracts were washed with brine, dried over anhydrous sodium sulfate, filtered and concentrated to give intermediate i-70a as a white solid.

(622) LCMS (ESI) m/z: [M+H]+=215.0.

Step 2: Preparation of 4-methyl-3-(methylsulfonyl)-N-(2-oxo-2-((4-phenylthiazol-2-yl)amino)ethyl)benzamide (Compound 19)

(623) ##STR00491##

(624) To a solution of intermediate i-70a (25 mg, 116.69 μmol) in DCM (1 mL) were added HATU (66.56 mg, 175.04 μmol) and DIPEA (75.41 mg, 583.46 μmol) and then 2-amino-N-(4-phenylthiazol-2-yl)acetamide (40.53 mg, 116.69 μmol) was added. A precipitate was formed after stirring at 30° C. for 2 hr. The precipitate was collected by filtration and lyophilized to afford compound 19 as a white solid.

(625) .sup.1H NMR (400 MHz, DMSO-d6) δ=12.09-10.54 (br s, 1H), 9.20-9.19 (m, 1H), 8.44 (s, 1H), 8.13-7.10 (m, 1H), 7.91-7.89 (m, 2H), 7.63-7.60 (m, 2H), 7.45-7.40 (m, 2H), 7.35-7.32 (m, 1H), 4.22 (d, J=5.6 Hz, 2H), 3.27 (s, 3H), 2.71 (s, 3H) ppm.

(626) LCMS (ESI) m/z: [M+H].sup.+=430.3.

Example 71. Preparation of Compound 4-(dimethylsulfamoyl)-N-[(1S)-3-methylsulfanyl-1-[(4-phenylthiazol-2-yl) carbamoyl]propyl]thiophene-2-carboxamide (Compound 64)

(627) ##STR00492##

(628) To a solution of 4-(dimethylsulfamoyl)thiophene-2-carboxylic acid (40.00 mg, 170.01 μmol), DIPEA (87.89 mg, 680.04 μmol, 118.45 μL) and HATU (71.11 mg, 187.01 μmol) in DCM (1.0 mL) was added (2S)-2-amino-4-methylsulfanyl-N-(4-phenylthiazol-2-yl)butanamide (78.82 mg, 187.01 μmol) and the mixture was stirred at 30° C. for 2 hr. The reaction mixture was concentrated under reduced pressure to give a residue. The residue was purified by prep-HPLC (column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 45%-75%, 10 min) and lyophilized to give compound 64 as a white solid.

(629) 1H NMR (400 MHz, DMSO-d.sub.6) δ=12.60 (s, 1H), 9.09 (d, J=7.2 Hz, 1H), 8.46 (d, J=1.6 Hz, 1H), 8.27 (d, J=1.6 Hz, 1H), 7.92-7.90 (m, 2H), 7.67 (s, 1H), 7.46-7.42 (m, 2H), 7.35-7.33 (m, 1H), 4.77-4.73 (m, 1H), 2.73-2.67 (m, 6H), 2.66-2.53 (m, 2H), 2.10-2.03 (m, 5H) ppm.

(630) LCMS (ESI) m/z: [M+H].sup.+=525.0

Example 72. Preparation of 3-cyclopropylsulfonyl-N-[2-oxo-2-[(4-phenylthiazol-2-yl)amino]ethyl]benzamide (Compound 39)

(631) ##STR00493##

Step 1: Preparation of 1-cyclopropylsulfanyl-3-methyl-benzene (Intermediate i-72a)

(632) ##STR00494##

(633) A mixture of 3-methylbenzenethiol (100.0 mg, 805.13 μmol), bromocyclopropane (292.21 mg, 2.42 mmol) and t-BuOK (99.38 mg, 885.65 μmol) in DMSO (0.5 mL) was stirred at 100° C. for 12 hr. The reaction mixture was diluted with water (2.0 mL) and extracted with EtOAc (2.0 mL*3). The combined organic extracts were dried over Na.sub.2SO.sub.4, filtered and concentrated under reduced pressure to give a residue. The residue was purified by column chromatography (SiO.sub.2, Petroleum ether) to give intermediate i-72a as colorless oil.

(634) .sup.1H NMR (400 MHz, CDCl.sub.3) δ=7.18 (s, 1H), 7.10 (d, J=4.0 Hz, 3H), 6.88-6.86 (m, 1H), 2.29-2.25 (m, 3H), 2.14-2.09 (m, 1H), 1.01-0.96 (m, 2H), 0.63-0.60 (m, 2H) ppm.

Step 2: Preparation of 3-cyclopropylsulfonylbenzoic acid (Intermediate i-72b)

(635) ##STR00495##

(636) To a solution of intermediate i-72a (60.0 mg, 365.26 μmol) and KMnO.sub.4 (230.89 mg, 1.46 mmol) in water (1.0 mL) was added NaOH (7.30 mg, 182.63 μmol). The mixture was stirred at 100° C. for 16 hr. The reaction mixture was poured into sat. Na.sub.2SO.sub.3 (20.0 mL), then 1 N HCl (20.0 mL) was added to the mixture and extracted with EtOAc (20.0 mL*3). The combined organic extracts were washed with brine (20.0 mL), dried over Na.sub.2SO.sub.4, filtered and concentrated under reduced pressure to give the intermediate i-72b as yellow oil.

(637) .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ=8.35 (s, 1H), 8.26 (d, J=7.6 Hz, 1H), 8.13 (d, J=7.6 Hz, 1H), 7.82-7.78 (m, 1H), 2.99-2.93 (m, 1H), 1.17-1.06 (m, 4H) ppm.

(638) LCMS (ESI) m/z: [M+H].sup.+=227.0

Step 3: Preparation of 3-cyclopropylsulfonyl-N-[2-oxo-2-[(4-phenylthiazol-2-yl)amino]ethyl]benzamide (Compound 39)

(639) ##STR00496##

(640) To a solution of intermediate i-72b (30.0 mg, 132.60 μmol), HATU (60.50 mg, 159.12 μmol) and DIPEA (68.55 mg, 530.39 μmol) in DCM (1.0 mL) was added 2-amino-N-(4-phenylthiazol-2-yl) acetamide (50.66 mg, 145.86 μmol). Then the mixture was stirred at 30° C. for 4 hr. The reaction mixture was concentrated under reduced pressure to give a residue. The residue was triturated with MeOH (4.0 mL), the solid was collected by filtration and dried in vacuum to give a white solid. Then the solid was suspended in water (3.0 mL) and lyophilized to give compound 39 as a white solid.

(641) .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ=12.46 (s, 1H), 9.27 (m, 1H), 8.42 (s, 1H), 8.25 (d, J=8.0 Hz, 1H), 8.10 (d, J=8.4 Hz, 1H), 7.92-7.90 (m, 2H), 7.81-7.79 (m, 1H), 7.64 (s, 1H), 7.44-7.42 (m, 2H), 7.33-7.31 (m, 1H), 4.24 (d, J=5.6 Hz, 2H), 2.95-2.90 (m, 1H), 1.19-1.07 (m, 4H) ppm.

(642) LCMS (ESI) m/z: [M+H].sup.+=442.3.

Example 73. Preparation of 3-[2-[[2-[[3-(dimethylsulfamoyl)benzoyl]amino]acetyl]amino]thiazol-4-yl]-N,N-dimethyl-benzamide (compound 37)

(643) ##STR00497##

(644) To a solution of N-[2-[(4-bromothiazol-2-yl)amino]-2-oxo-ethyl]-3-(dimethylsulfamoyl)benzamide (50.0 mg, 111.78 μmol) and [3-(dimethylcarbamoyl)phenyl]boronic acid (64.72 mg, 335.33 μmol) in dioxane (3.0 mL) and H.sub.2O (0.3 mL) were added Pd(t-Bu.sub.3P).sub.2 (28.56 mg, 55.89 μmol) and DIEA (72.23 mg, 558.88 μmol, 97.34 μL) under N.sub.2. The mixture was stirred at 100° C. for 2 hrs. The solution was cooled to 30° C. and a solid formed. The mixture was filtered to give the crude product as a solid. The crude product was dissolved in MeOH (1.0 mL) and DMSO (1.0 mL), purified by Pre-HPLC (column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 30%-60%, 9 min) and lyophilized to give compound 37 as a white solid.

(645) .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ=12.47 (s, 1H), 9.30-9.29 (m, 1H), 8.27-8.23 (m, 2H), 7.98-7.93 (m, 3H), 7.81-7.75 (m, 2H), 7.50-7.52 (m, 1H), 7.34 (d, J=7.60 Hz, 1H), 4.25 (d, J=5.6 Hz, 2H), 3.01-2.94 (m, 6H), 2.66 (s, 6H) ppm.

(646) LCMS (ESI) m/z: [M+H].sup.+=516.0.

Example 74. Preparation of 3-methylsulfonyl-N-[2-oxo-2-[[4-(3-phenylphenyl)thiazol-2-yl]amino]ethyl]benzamide (Compound 36)

(647) ##STR00498##

Step 1: Preparation of tert-butyl N-[2-[(4-bromothiazol-2-yl)amino]-2-oxo-ethyl]carbamate (Intermediate i-74a)

(648) ##STR00499##

(649) To a solution of 4-bromothiazol-2-amine (3.0 g, 16.76 mmol) and 2-(tert-butoxycarbonylamino) acetic acid (4.40 g, 25.13 mmol) in pyridine (30.0 mL) was added EDCl (16.06 g, 83.78 mmol). The mixture was stirred at 15° C. for 16 hr. The reaction mixture was then concentrated under reduced pressure. The residue was diluted with 0.5N HCl (100.0 mL) and extracted with EtOAc (50 mL*3). The combined organic extracts were washed with NaHCO.sub.3 (50.0 mL) and brine (50.0 mL), dried over Na.sub.2SO.sub.4, filtered and concentrated under reduced pressure to give a residue. The residue was purified by column chromatography (SiO.sub.2, Petroleum ether/Ethyl acetate=7/1 to 3:1) to give intermediate i-74a as a white solid.

(650) LCMS (ESI) m/z: [M+Na].sup.+=358.1

Step 2: Preparation of 2-amino-N-(4-bromothiazol-2-yl)acetamide (Intermediate i-74b)

(651) ##STR00500##

(652) A mixture of intermediate i-74a (300 mg, 892.31 μmol) in DCM (5.0 mL) and TFA (1.0 mL) was stirred at 30° C. for 2 hrs. The mixture was diluted with DCM (20.0 mL) and concentrated under vacuum. This operation was repeated three times. The residue was washed with MTBE (5 mL*2) to give intermediate i-74b (TFA salt) as yellow oil which was used directly in the next step.

(653) LCMS (ESI) m/z: [M+Na].sup.+=236.1

Step 3: Preparation of N-[2-[(4-bromothiazol-2-yl)amino]-2-oxo-ethyl]-3-methylsulfonyl-benzamide (Intermediate i-74c)

(654) ##STR00501##

(655) To a solution of 3-methylsulfonylbenzoic acid (171.55 mg, 856.87 μmol) in DMF (10.0 mL) were added HATU (977.42 mg, 2.57 mmol) and DIEA (553.72 mg, 4.28 mmol). The mixture was stirred at 30° C. for 30 min. Then intermediate i-74b (300.0 mg, 856.87 μmol) was added and the mixture was stirred at 30° C. for another 2 hrs. The mixture was poured into H.sub.2O (100.0 mL) and extracted with EtOAc (10.0 mL*3). The combined organic layers were washed with H.sub.2O (5.0 mL*3) and brine (5.0 mL*2), then dried over Na.sub.2SO.sub.4, filtered and concentrated under reduced pressure. The residue was purified by Pre-HPLC (column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 32%-62%, 9 min) and lyophilized to give intermediate i-74c as a light yellow solid.

(656) LCMS (ESI) m/z: [.sup.81BrM+H].sup.+=419.9.

Step 4: Preparation of 3-methylsulfonyl-N-[2-oxo-2-[[4-(3-phenylphenyl)thiazol-2-yl]amino]ethyl]benzamide (Compound 36)

(657) ##STR00502##

(658) To a solution of intermediate i-74c (60.0 mg, 143.44 μmol) and (3-phenylphenyl)boronic acid (85.22 mg, 430.33 μmol) in dioxane (3.0 mL) and H.sub.2O (0.15 mL) were added Pd(t-Bu.sub.3P).sub.2 (36.65 mg, 71.72 μmol) and DIEA (92.69 mg, 717.21 μmol, 124.92 μL). The mixture was stirred at 100° C. for 2 hrs. The mixture was cooled to 30° C. and a solid formed. The mixture was filtered to give crude product as yellow solid, which was dissolved in MeOH (1.0 mL) and DMSO (1.0 mL) and purified by Pre-HPLC (column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 45%-75%, 9 min) and lyophilized to give compound 36 as a white solid.

(659) .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ=12.49 (s, 1H), 9.27-9.25 (m, 1H), 8.46 (s, 1H), 8.25-8.21 (m, 2H), 8.13 (d, J=7.80 Hz, 1H), 7.90 (d, J=7.80 Hz, 1H), 7.81-7.79 (m, 2H), 7.73-7.71 (m, 2H), 7.65-7.60 (m, 1H), 7.54-7.49 (m, 3H), 7.48-7.40 (m, 1H), 4.25 (d, J=5.6 Hz, 2H), 3.29 (s, 3H) ppm LCMS (ESI) m/z: [M+H].sup.+=492.0.

Example 75. Preparation of 4-(methanesulfonamido)-N-[(1S)-3-methylsulfanyl-1-[(4-phenylthiazol-2-yl)carbamoyl]propyl]benzamide (Compound 38)

(660) ##STR00503##

(661) To a mixture of (2S)-2-amino-4-methylsulfanyl-N-(4-phenylthiazol-2-yl)butanamide (50.0 mg, 118.64 μmol) and 4-(methanesulfonamido)benzoic acid (30.64 mg, 142.36 μmol) in pyridine (1 mL) was added EDCl (68.23 mg, 355.91 μmol). The mixture was stirred at 30° C. for 2 h. The reaction mixture was concentrated under reduced pressure to give a residue. The residue was purified by Prep-HPLC (column: Boston Green ODS 150*30 5μ; mobile phase: [water (0.1% TFA)-ACN]; B %: 42%-72%, 9 min) and lyophilized to give compound 38 as a white solid.

(662) .sup.1H NMR (400 MHz, MeOD-d.sub.4) δ=7.91-7.88 (m, 4H), 7.40-7.29 (m, 6H), 4.92-4.88 (m, 1H), 3.03 (s, 3H), 2.70-2.63 (m, 2H), 2.30-2.20 (m, 2H), 2.13 (s, 3H) ppm.

(663) LCMS (ESI) m/z: [M+H].sup.+=505.3.

Example 76. Preparation of 3-(dimethylsulfamoyl)-N-[2-[(4-isopropylthiazol-2-yl)amino]-2-oxoethyl]benzamide (Compound 35)

(664) ##STR00504##

(665) To a solution of 2-[[3-(dimethylsulfamoyl)benzoyl]amino]acetic acid (50.0 mg, 174.64 μmol), 4-isopropylthiazol-2-amine (24.84 mg, 174.64 μmol) in DCM (1.0 mL) were added DIEA (67.71 mg, 523.92 μmol, 91.26 μL), EDCl (50.22 mg, 261.96 μmol) and HOBt (35.40 mg, 261.96 μmol). The mixture was stirred at 15° C. for 16 hr. The reaction mixture was concentrated in vacuo to afford a residue. The residue was purified by prep-HPLC (FA conditions) to afford compound 35 as a white solid.

(666) .sup.1HNMR (400 MHz, DMSO-d.sub.6) δ=12.26 (br s, 1H), 9.26-9.24 (m, 1H), 8.26-8.22 (m, 2H), 7.94 (d, J=8.0 Hz, 1H), 7.81-7.78 (m, 1H), 6.75 (s, 1H), 4.17 (d, J=5.6 Hz, 2H), 2.96-2.90 (m, 1H), 2.65 (s, 6H), 1.22 (d, J=6.8 Hz, 6H) ppm.

(667) LCMS (ESI) m/z: [M+H].sup.+=411.1.

Example 77. Preparation of N-[2-[(4-cyclohexylthiazol-2-yl)amino]-2-oxo-ethyl]-3-(dimethylsulfamoyl)benzamide (Compound 34)

(668) ##STR00505##

(669) To a solution of 2-[[3-(dimethylsulfamoyl)benzoyl]amino]acetic acid (50 mg, 174.64 μmol), 4-cyclohexylthiazol-2-amine (31.83 mg, 174.64 μmol) in DCM (2.0 mL) were added DIEA (67.71 mg, 523.92 μmol, 91.26 μL), EDCl (50.22 mg, 261.96 μmol) and HOBt (35.40 mg, 261.96 μmol). The mixture was stirred at 15° C. for 16 hr. The reaction mixture was concentrated invacuo to afford a residue. The residue was purified by prep-HPLC (FA conditions) to afford compound 34 as a yellow solid.

(670) 1H NMR (400 MHz, DMSO-d.sub.6) δ=12.23 (br s, 1H), 9.26-9.24 (m, 1H), 8.25-8.21 (m, 2H), 7.94 (d, J=8.0 Hz, 1H), 7.81-7.77 (m, 1H), 6.72 (s, 1H), 4.17 (d, J=5.6 Hz, 2H), 2.64 (s, 6H), 2.59-2.56 (m, 1H), 1.94-1.92 (m, 2H), 1.76-1.73 (m, 3H), 1.39-1.31 (m, 4H), 1.18-1.15 (m, 1H) ppm.

(671) LCMS (ESI) m/z: [M+H].sup.+=451.0.

Example 78. Preparation of 3-[2-[[2-[[3-(dimethylsulfamoyl)benzoyl]amino]acetyl]amino]thiazol-4-yl]benzoic acid (Compound 331

(672) ##STR00506##

Step 1: Preparation of methyl 3-[2-[[2-[[3-(dimethylsulfamoyl)benzoyl]amino]acetyl]amino]thiazol-4-yl]benzoate (Intermediate i-78a)

(673) ##STR00507##

(674) To a solution of N-[2-[(4-bromothiazol-2-yl)amino]-2-oxo-ethyl]-3-(dimethylsulfamoyl)benzamide (30.0 mg, 67.07 μmol) and (3-methoxycarbonylphenyl)boronic acid (36.21 mg, 201.21 μmol) in dioxane (2.0 mL) and H.sub.2O (0.1 mL) were added Pd(t-Bu.sub.3P).sub.2 (17.14 mg, 33.53 μmol) and DIEA (43.34 mg, 335.35 μmol, 58.41 μL) under N.sub.2. The mixture was stirred at 100° C. for 2 hrs. A solid was formed upon cooling to 30° C. The solid was filtered and triturated in EtOAc (3 mL), and then dried in vacuum to give intermediate i-78a as a light yellow solid which was used directly in the next step.

(675) LCMS (ESI) m/z: [M+H].sup.+=503.2.

Step 2: Preparation of 3-[2-[[2-[[3-(dimethylsulfamoyl)benzoyl]amino]acetyl]amino]thiazol-4-yl]benzoic acid (Compound 34)

(676) ##STR00508##

(677) To a solution of intermediate i-78a (15.0 mg, 29.85 μmol) in MeOH (2.0 mL) and H.sub.2O (1.0 mL) was added NaOH (1.19 mg, 29.85 μmol). The mixture was stirred at 30° C. for 12 hr then diluted with MeOH (10.0 mL) and treated with HCl (1 M) to pH 5-6. The mixture was concentrated in vacuo to give a residue. The residue was purified through Pre-HPLC (column: Phenomenex Synergi C18 150*25*10 μm; mobile phase: [water (0.1% TFA)-ACN]; B %: 28%-58%, 9 min) and lyophilized to give compound 34 as an off-white solid.

(678) .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ 13.02 (br s, 1H), 12.53 (s, 1H), 9.31-9.30 (m, 1H), 8.54 (s, 1H), 8.27-8.24 (m, 2H), 8.13 (d, J=8.0 Hz, 1H), 7.94 (d, J=7.6 Hz, 1H), 7.90 (d, J=7.60 Hz, 1H), 7.83-7.81 (m, 2H), 7.77-7.57 (m, 1H), 4.25 (d, J=5.6 Hz, 2H), 2.66 (s, 6H) ppm.

(679) LCMS (ESI) m/z: [M+H].sup.+=488.9.

Example 79. Preparation of 3-(hydroxymethyl)-N-[(1S)-3-methylsulfanyl-1-[(4-phenylthiazol-2-yl)carbamoyl]propyl]benzamide (Compound 84)

(680) ##STR00509##

(681) To a solution of 3-(hydroxymethyl)benzoic acid (14.85 mg, 97.58 μmol) and (2S)-2-amino-4-methylsulfanyl-N-(4-phenylthiazol-2-yl)butanamide (30.00 mg, 97.58 μmol) in DCM (0.5 mL) were added DIPEA (37.83 mg, 292.75 μmol) and HATU (55.66 mg, 146.37 μmol) at 25° C. The mixture was stirred at 25° C. for 16 hr. The reaction mixture was concentrated under reduced pressure to give a residue. The residue was purified by prep-HPLC (FA condition) to afford compound 84 as a white solid.

(682) LCMS (ESI) m/z: [M+H].sup.+=442.1.

(683) .sup.1HNMR (400 MHz, METHANOL-d.sub.4): δ=7.90-7.88 (m, 3H), 7.80 (d, J=8.0 Hz, 1H), 7.56 (d, J=7.6 Hz, 1H), 7.48-7.46 (m, 1H), 7.40-7.36 (m, 3H), 7.29 (m, 1H), 4.94-4.90 (m, 1H), 4.68 (m, 2H), 2.71-2.64 (m, 2H), 2.28-2.22 (m, 2H), 2.13 (s, 3H) ppm.

Example 80. Preparation of 4-(hydroxymethyl)-N-[(1S)-3-methylsulfanyl-1-[(4-phenylthiazol-2-yl)carbamoyl]propyl]benzamide (Compound 85)

(684) ##STR00510##

(685) To a solution of 4-(hydroxymethyl)benzoic acid (14.85 mg, 97.58 μmol) and (2S)-2-amino-4-methylsulfanyl-N-(4-phenylthiazol-2-yl)butanamide (30.00 mg, 97.58 μmol) in DCM (0.5 mL) were added DIPEA (37.83 mg, 292.75 μmol) and HATU (55.66 mg, 146.37 μmol) at 25° C. The mixture was stirred at 25° C. for 16 hr. The reaction mixture was concentrated under reduced pressure to give a residue. The residue was purified by prep-HPLC (FA condition) to afford compound 85 as a white solid.

(686) .sup.1HNMR (DMSO-d.sub.6, 400 MHz): δ=12.37 (br s, 1H), 8.68 (d, J=7.2 Hz, 1H), 7.92-7.89 (m, 4H), 7.63 (s, 1H), 7.45-7.42 (m, 4H), 7.35-7.33 (m, 1H), 5.32 (br s, 1H), 4.79-4.73 (m, 1H), 4.57 (m, 2H), 2.59-2.50 (m, 3H), 2.15-2.10 (m, 5H) ppm.

(687) LCMS (ESI) m/z: [M+H].sup.+=441.9.

Example 81. Preparation of 4-amino-N-[(1S)-1-methyl-2-oxo-2-[(4-phenylthiazol-2-yl)amino]ethyl]benzamide (Compound 82)

(688) ##STR00511##

Step 1: Preparation of tert-butyl N-[(1S)-1-methyl-2-oxo-2-[(4-phenylthiazol-2-yl)amino]ethyl]carbamate (Intermediate i-81a)

(689) ##STR00512##

(690) To a mixture of 4-phenylthiazol-2-amine (200.0 mg, 1.13 mmol) and (2S)-2-(tert-butoxycarbonylamino)propanoic acid (214.72 mg, 1.13 mmol) in DCM (2.0 mL) were added DIPEA (440.00 mg, 3.40 mmol) and HATU (647.24 mg, 1.70 mmol) in one portion at 25° C. under N.sub.2. The mixture was stirred at 25° C. for 2 hrs. The mixture was poured into water (10.0 mL) and stirred for 5 min. The aqueous phase was extracted with DCM (10.0 mL*2). The combined organic extracts were washed with brine (10.0 mL), dried over anhydrous Na.sub.2SO.sub.4, filtered and concentrated in vacuum to afford Intermediate i-81a as light yellow oil which was used in the next step without purification.

(691) LCMS (ESI) m/z: [M+H].sup.+=348.1.

Step 2: Preparation of (S)-2-amino-N-(4-phenylthiazol-2-yl)propanamide hydrochloride (Intermediate i-81b)

(692) ##STR00513##

(693) To a mixture of Intermediate i-81a (500 mg, 1.44 mmol) in dioxane (5.0 mL) was added HCl/dioxane (4 M, 1.80 mL) in one portion at 25° C. The mixture was stirred at 25° C. for 30 min and a solid precipitated. The mixture was filtered and the filter cake was washed with EtOAc (10 mL). Then the solid was dried in vacuum to give intermediate i-81b (HCl salt) as a white solid.

(694) .sup.1H NMR (400 MHz, D20) 6=7.84-7.79 (m, 1H), 7.49-7.43 (m, 1H), 4.39-4.35 (m, 1H), 2.80 (s, 1H), 1.67 (d, J=6.8 Hz, 3H).

(695) Chiral HPLC: OD-3_5CM_MeOH (DEA)_5_40_3_ML_T35.

(696) LCMS (ESI) m/z: [M+H].sup.+=248.1.

Step 3: Preparation 4-amino-N-[(1S)-1-methyl-2-oxo-2-[(4-phenylthiazol-2-yl)amino]ethyl]benzamide (Compound 82)

(697) ##STR00514##

(698) To a mixture of Intermediate i-81b (50 mg, 176.19 μmol) and 4-aminobenzoic acid (24.16 mg, 176.19 μmol) in DCM (1.0 mL) were added DIPEA (91.09 mg, 704.78 μmol) and HATU (100.49 mg, 264.29 μmol) in one portion at 25° C. The mixture was stirred at 25° C. for 12 hr. The mixture was concentrated in vacuum to afford a residue. The residue was purified by reverse phase column (FA conditions) to afford compound 82 as a white solid.

(699) .sup.1H NMR (400 MHz, METHANOL-d.sub.4) δ=7.88 (d, J=7.2 Hz, 2H), 7.69 (d, J=8.6 Hz, 2H), 7.40-7.37 (m, 3H), 7.31-7.27 (m, 1H), 6.68 (d, J=8.6 Hz, 2H), 4.77-4.72 (m, 1H), 1.56 (d, J=7.2 Hz, 3H) ppm.

(700) LCMS (ESI) m/z: [M+H].sup.+=367.0.

(701) Chiral HPLC: Amycoat_MeOH+CAN(DEA)_40_3 mL-35 T, 1.474 min.

Example 82. Preparation of 4-(methylamino)-N-[(1S)-3-methylsulfanyl-1-[(4-phenylthiazol-2-yl)carbamoyl]propyl]benzamide (Compound 81)

(702) ##STR00515##

(703) To a solution of 4-(methylamino)benzoic acid (10.76 mg, 71.18 μmol) in DCM (0.5 mL) were added DIPEA (27.60 mg, 213.55 μmol) and HATU (40.60 mg, 106.77 μmol) at 25° C. The mixture was stirred at 25° C. for 1 hr. To the reaction mixture was added (2S)-2-amino-4-methylsulfanyl-N-(4-phenylthiazol-2-yl)butanamide (30 mg, 71.18 μmol). The reaction mixture was stirred at 25° C. for 16 hr. Then the mixture was concentrated under reduced pressure to give a residue. The residue was purified through reverse phase-HPLC (FA condition) and followed by prep-TLC (PE/EA=1/1) to afford compound 81 as a white solid.

(704) .sup.1H NMR (400 MHz, METHANOL-d.sub.4) δ=7.89 (d, J=7.6 Hz, 2H), 7.74 (d, J=8.4 Hz, 2H), 7.40 (m, 3H), 7.30 (m, 1H), 6.61 (d, J=8.8 Hz, 2H), 4.89 (m, 1H), 2.81 (s, 3H), 2.69 (m, 2H), 2.26 (m, 2H), 2.12 (s, 3H) ppm.

(705) LCMS (ESI) m/z: [M+H].sup.+=441.0.

(706) Chiral HPLC: Cellucoat-MeOH+ACN(DEA)-40-5 min-3 mL-35T, 1.895 min.

Example 83. Preparation of N-[(1S)-3-methylsulfanyl-1-[(4-phenylthiazol-2-yl)carbamoyl]propyl]indoline-5-carboxamide (Compound 80)

(707) ##STR00516##

(708) To a solution of indoline-5-carboxylic acid (11.61 mg, 71.18 μmol) in DCM (0.5 mL) were added DIPEA (27.60 mg, 213.55 μmol) and HATU (40.60 mg, 106.77 μmol) at 25° C. The mixture was stirred at 25° C. for 1 hr and then (2S)-2-amino-4-methylsulfanyl-N-(4-phenylthiazol-2-yl) butanamide (30.0 mg, 71.18 μmol) was added. The reaction mixture was stirred at 25° C. for 16 hr. The reaction mixture was concentrated in vacuum to give a residue. The residue was purified through reverse phase-HPLC (FA condition) and followed by prep-TLC (PE/EA=1/1) to afford compound 80 as a white solid.

(709) .sup.1HNMR (400 MHz, MeOD) δ=7.88-7.86 (m, 2H), 7.63-7.59 (m, 2H), 7.39-7.36 (m, 3H), 7.28-7.26 (m, 1H), 6.58-6.52 (m, 1H), 4.89-4.86 (m, 1H), 3.59-3.54 (m, 2H), 3.04-3.00 (m, 2H), 2.68-2.63 (m, 2H), 2.20-2.12 (m, 2H), 2.11 (s, 3H) ppm.

(710) LCMS (ESI) m/z: [M+H].sup.+=453.0.

(711) Chiral HPLC: Cellucoat-MeOH+ACN(DEA)-40-5 min-3 mL-35T, 1.444 min.

Example 84. Preparation of (S)—N-(4-(methylthio)-1-oxo-1-((4-phenylthiazol-2-yl)amino)butan-2-yl)-1H-indole-5-carboxamide (Compound 83)

(712) ##STR00517##

(713) To a solution of 1H-indole-5-carboxylic acid (19.12 mg, 118.64 μmol) in DCM (1.0 mL) were added DIPEA (46.00 mg, 355.91 μmol), HATU (67.66 mg, 177.95 μmol) and followed by (2S)-2-amino-4-methylsulfanyl-N-(4-phenylthiazol-2-yl)butanamide (36.47 mg, 118.64 μmol). The reaction mixture was stirred at 30° C. for 2 hr. Then the reaction mixture was poured into water (5.0 mL) and extracted by EtOAc (5.0 mL×2). The combined organic layers were washed with brine, dried over anhydrous sodium sulfate, filtered and concentrated to give a residue. The residue was purified by prep-HPLC (FA condition) to afford compound 83 as a brown solid.

(714) 1H NMR (400 MHz, DMSO-d.sub.6) δ=12.43 (br s, 1H), 11.34 (s, 1H), 8.56 (d, J=7.2 Hz, 1H), 8.24 (s, 1H), 7.91-7.89 (m, 2H), 7.70-7.68 (m, 1H), 7.63 (s, 1H), 7.44-7.41 (m, 4H), 7.32-7.31 (m, 1H), 6.56 (s, 1H), 4.79-4.74 (m, 1H), 2.68-2.65 (m, 1H), 2.59-2.55 (m, 1H), 2.14-2.12 (m, 2H), 2.10 (s, 3H) ppm.

(715) LCMS (ESI) m/z: [M+H].sup.+=451.3.

(716) Chiral SFC: OD-3_5 CM_MeOH (DEA)_40_3 ML_T35.M, 2.392 min.

Example 85. Preparation of (S)—N-(4-(methylthio)-1-oxo-1-((4-phenylthiazol-2-yl)amino)butan-2-yl)-1H-benzo[d]imidazole-5-carboxamide (Compound 66)

(717) ##STR00518##

(718) To a solution of 1H-benzimidazole-5-carboxylic acid (19.24 mg, 118.64 μmol) in DCM (1.0 mL) were added DIPEA (46.00 mg, 355.91 μmol), HATU (67.66 mg, 177.95 μmol) and then (2S)-2-amino-4-methylsulfanyl-N-(4-phenylthiazol-2-yl)butanamide (36.47 mg, 118.64 μmol) was added. The reaction mixture was stirred at 30° C. for 2 hrs. The reaction mixture was poured into water (5.0 mL) and extracted with EtOAc (5.0 mL×2). The combined organic extracts were washed with brine, dried over anhydrous sodium sulfate, filtered and concentrated to give a residue. The residue was purified by prep-HPLC (FA condition) to afford compound 66 as a yellow solid.

(719) .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ=12.50 (br s, 1H), 8.73 (d, J=7.2 Hz, 1H), 8.34 (s, 1H), 8.25 (s, 1H), 7.91-7.81 (m, 2H), 7.80-7.79 (m, 1H), 7.62-7.61 (m, 2H), 7.44-7.40 (m, 2H), 7.32-7.31 (m, 1H), 4.80-4.75 (m, 1H), 2.68-2.65 (m, 1H), 2.59-2.56 (m, 1H), 2.17-2.14 (m, 2H), 2.10 (s, 3H) ppm.

(720) LCMS (ESI) m/z: [M+H].sup.+=452.3.

(721) Chiral SFC: Cellucoat-MeOH (DEA)-40-3 mL-35 T, 0.942 min.

Example 86. Synthesis of Compound 87

(722) Compound 87 was synthesized by the method illustrated in the below scheme:

(723) ##STR00519##

Preparation of (S)-4-amino-N-(4-(methylthio)-1-oxo-1-((4-(3-(pyridin-4-yl)phenyl)thiazol-2-yl)amino)butan-2-yl)benzamide (Compound 87)

(724) ##STR00520##

Step 1: Preparation of 2-bromo-1-(3-bromophenyl)ethenone (Intermediate B)

(725) ##STR00521##

(726) To a solution of 1-(3-bromophenyl)ethanone (200 g, 1.00 mol, 132.45 mL) in CHCl.sub.3 (250 mL) was added Br.sub.2 (240.86 g, 1.51 mol, 77.70 mL) under N.sub.2 dropwise at 20° C. The mixture was stirred at 80° C. for 1 hr. The reaction mixture was concentrated to give intermediate B (279.27 g, crude) as yellow oil, which was used for next step directly.

Step 2: Preparation of 4-(3-bromophenyl)thiazol-2-amine (Intermediate C)

(727) ##STR00522##

(728) To a solution of thiourea (229.23 g, 3.01 mol) in EtOH (1.5 L) was added intermediate B (279 g, 1.00 mol). The mixture was stirred at 85° C. for 2 hr. Then the mixture was concentrated in vacuum to give a residue. The residue was poured into NaHCO.sub.3 (2 L) solution to adjust pH to 8, then the solution was extracted with EA (2 L*3). The combined organic layers were washed with brine (4 L), dried over Na.sub.2SO.sub.4 and filtered. The filtrate was concentrated to give a residue. The residue was purified by column chromatography (SiO.sub.2. PE:EA=5:1) to give intermediate C (130 g, 494.40 mmol, 49.25% yield) as a yellow solid. LCMS (ESI) m/z: [M+H].sup.+=257.0. .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ=7.98 (m, 1H), 7.79 (d, J=8.0 Hz, 1H), 7.44-7.42 (m, 1H), 7.33-7.29 (m, 1H), 7.14 (s, 1H), 7.09 (s, 2H) ppm.

Step 3: Preparation of 4-[3-(4-pyridyl)phenyl]thiazol-2-amine (Intermediate E)

(729) ##STR00523##

(730) 4-(3-bromophenyl)thiazol-2-amine (20 g, 78.40 mmol), 4-pyridylboronic acid (28.9 g, 239.18 mmol), ditert-butyl(cyclopentyl)phosphane; dichloropalladium; iron (2.56 g, 3.92 mmol) and K.sub.3PO.sub.4 (66.56 g, 313.56 mmol) were taken up in dioxane (240 mL) and H.sub.2O (24 mL). The mixture was purged with N.sub.2 three times and then stirred at 80° C. for 7 hr. Water (800 mL) was added and the mixture was extracted with EtOAc (800 mL*3). The combined organic layers were washed with brine, dried over anhydrous Na.sub.2SO.sub.4, filtered and concentrated under vacuum. The residue was added into DCM (30 mL) and MTBE (100 mL) and then stirred for 5 min. The precipitate was collected through filtration and washed with MTBE (10 mL) to give the intermediate E (16.2 g, 61.17 mmol, 78.03% of yield) as a yellow solid.

(731) LCMS (ESI) m/z: [M+H].sup.+=254.0.

Step 4: Preparation of tert-butyl N-[(1S)-3-methylsulfanyl-1-[[4-[3-(4-pyridyl)phenyl]thiazol-2-yl]carbamoyl]propyl]carbamate (Intermediate G)

(732) ##STR00524##

(733) To a mixture of intermediate E (12.6 g, 49.74 mmol) and (2S)-2-(tert-butoxycarbonylamino)-4-methylsulfanyl-butanoic acid (18.6 g, 74.61 mmol) in DCM (900 mL) was added EEDQ (24.6 g, 99.48 mmol) and the mixture was stirred at 25° C. for 2 hr. The solvent was removed in vacuum. The residue was triturated with DCM (100 mL) followed by MeOH (200 mL) to give the intermediate G (11.7 g, 23.73 mmol, 47.71% yield, ee %=99.44%) as a white solid. LCMS (ESI) m/z: [M+H].sup.+=485.1. .sup.1H NMR (400 MHz, DMSO) δ=12.39 (s, 1H), 8.68-8.66 (m, 2H), 8.30 (s, 1H), 8.02-7.99 (m, 1H), 7.83 (s, 1H), 7.76-7.74 (m, 3H), 7.61-7.57 (m, 1H), 7.28 (d, J=7.6 Hz, 1H), 4.31-4.30 (m, 1H), 2.65-2.44 (m, 2H), 2.06 (s, 3H) 2.01-1.85 (m, 2H), 1.38 (s, 9H) ppm. Note: The key point of this reaction is the concentration. (1 mmol E with 17-18 mL DCM was the best condition)

Step 5: Preparation of (2S)-2-amino-4-methylsulfanyl-N-[4-[3-(4-pyridyl)phenyl]thiazol-2-yl]butanamide (Intermediate H)

(734) ##STR00525##

(735) A mixture of intermediate G (11.5 g, 23.73 mmol) in MeOH (50 mL) was added HCl/dioxane (4 M, 100 mL) and the mixture was stirred at 25° C. for 1 hr. The mixture was poured into MTBE (1000 mL) and the precipitate was collected by filtration to give the intermediate H (9.99 g, 23.73 mmol, 100.00% yield, HCl salt) as a yellow solid. LCMS (ESI) m/z: [M+H].sup.+=385.0

Step 6: Preparation of 4-amino-N-[(1S)-3-methylsulfanyl-1-[[4-[3-(4-pyridyl)phenyl]thiazol-2-yl]carbamoyl]propyl]benzamide (Intermediate E)

(736) ##STR00526##

(737) To a mixture of intermediate H (4 g, 9.50 mmol) and 4-aminobenzoic acid (1.30 g, 9.50 mmol) in DMF (40 mL) was added DIEA (4.91 g, 38.01 mmol, 6.62 mL), EDCl (2.73 g, 14.25 mmol) and HOBt (1.93 g, 14.25 mmol). The solution was stirred at 25° C. for 14 hr and then poured into water (200 mL) and the precipitate was collected by filtration. The solid was triturated in MeOH (200 mL) (a gelatinous solid was formed). The precipitate was collected by filtration and the solid was further purified by column chromatography (SiO.sub.2, DCM:MeOH=80:1-20:1) to give Compound 87 (2.13 g, 4.19 mmol, 44.11% yield, ee %=99.28%) as a white solid. LCMS (ESI) m/z: [M+H].sup.+=504.0. .sup.1H NMR (400 MHz, DMSO) δ=12.40 (s, 1H), 8.68-8.66 (m, 2H), 8.31-8.30 (m, 1H), 8.22 (d, J=7.2 Hz, 1H), 8.02-7.99 (m, 1H), 7.82 (s, 1H), 7.76-7.74 (m, 3H), 7.67-7.63 (m, 2H), 7.61-7.57 (m, 1H), 6.58-6.54 (m, 2H), 5.67 (s, 2H), 4.72-4.67 (m, 1H), 2.65-2.54 (m, 2H), 2.12-2.06 (m, 5H) ppm.

Example 87. Experimental Data for Compounds of the Invention

(738) TABLE-US-00005 LCMS m/z: # Compound Name M + H HNMR 88 3-(methylsulfonyl)-N-(2-oxo-2-((4-(1- 483.1 .sup.1H NMR (400 MHz, DMSO-d6) δ = 12.57 oxo-1,2-dihydroisoquinolin- (br s, 1H), 11.25 (br s, 1H), 7-yl)thiazol-2- 9.29 (br s, 1H), 8.78 (br s, 1H), 8.46 (br s, yl)amino)ethyl)benzamide 1H), 8.32-8.13 (m, 3H), 7.79-7.71 (m, 3H), 7.19 (br s, 1H), 6.56 (br s, 1H), 4.25 (br s, 2H), 3.29 (br s, 3H) ppm. 89 3-(methylsulfonyl)-N-(2-oxo-2-((4-(3- 493.0 1H NMR (400 MHz, DMSO-d6) δ = 12.50 (pyridin-4-yl)phenyl)thiazol- (br s, 1H), 9.26 (br s, 1H), 2-yl)amino)ethyl)benzamide 8.68 (br s, 2H), 8.46 (br s, 1H), 8.31 (br s, 1H), 8.24 (br d, J = 8.4 Hz, 1H), 8.13 (br d, J = 6.8 Hz, 1H), 8.02 (br d, J = 7.6 Hz, 1H), 7.85-7.76 (m, 5H), 7.62-7.58 (m, 1H), 4.26 (br d, J = 4.8 Hz, 2H), 3.29 (br s, 3H) ppm. 90 3-(methylsulfonyl)-N-(2-oxo-2-((4-(3- 494.1 1H NMR (400 MHz, DMSO-d6) δ = 12.59 (pyrimidin-4-yl)phenyl)thiazol- (br s, 1H), 9.29 (br s, 2H), 8.91-8.82 (m, 2-yl)amino)ethyl)benzamide 2H), 8.45 (br s, 1H), 8.25-8.09 (m, 5H), 7.82 (br s, 2H), 7.62 (br s, 1H), 4.25 (br s, 2H), 3.28 (br s, 3H) ppm 91 3-(methylsulfonyl)-N-(2-oxo-2-((4-(3- 500.1 1H NMR (400 MHz, DMSO-d6) δ = 12.46 (tetrahydro-2H-pyran-4- (br s, 1H), 9.27-9.24 (m, 1H), 8.45 (s, 1H), yl)phenyl)thiazol-2- 8.23 (d, J = 8.0 Hz, 1H), 8.12 (d, J = 8.0 Hz, yl)amino)ethyl)benzamide 1H), 7.82-7.78 (m, 2H), 7.73 (d, J = 8.0 Hz, 1H), 7.65 (s, 1H), 7.38-7.34 (m, 1H), 7.22 (d, J = 7.60 Hz, 1H), 4.24 (d, J = 6.0 Hz, 2H), 3.98-3.95 (m, 2H), 3.48-3.42 (m, 2H), 3.28 (s, 3H), 2.85-2.77 (m, 1H), 1.74-1.65 (m, 4H) ppm 92 N-(2-((4-(2,3- 474.3 1H NMR (400 MHz, DMSO-d6) δ = 12.40 dihydrobenzo[b][1,4]dioxin- (br s, 1H), 9.25-9.23 (m, 1H), 6-yl)thiazol-2-yl)amino)-2-oxoethyl)-3- 8.45-8.44 (m, 1H), 8.23 (d, J = 8.0 Hz, 1H), (methylsulfonyl)benzamide 8.15-8.10 (m, 1H), 7.82-7.80 (m, 1H), 7.47 (s, 1H), 7.40-7.34 (m, 2H), 6.89 (d, J = 8.2 Hz, 1H), 4.27 (s, 4H), 4.23 (d, J = 5.8 Hz, 2H), 3.28 (s, 3H) ppm 93 N-(2-((4-(3-methyl-4-oxo-3,4- 498.0 1H NMR (400 MHz, DMSO-d6) δ = 12.57 dihydroquinazolin-6-yl)thiazol-2- (s, 1H), 9.27-9.25 (m, 1H), yl)amino)-2-oxoethyl)-3- 8.73 (d, J = 2.0 Hz, 1H), 8.46-8.45 (m, 1H), (methylsulfonyl)benzamide 8.37 (s, 1H), 8.34-7.32 (m, 1H), 8.24 (d, J = 7.6 Hz, 1H), 8.13 (d, J = 8.4 Hz, 1H), 7.85 (s, 1H), 7.82-7.79 (m, 1H), 7.72 (d, J = 8.4 Hz, 1H), 4.26 (d, J = 6.0 Hz, 2H), 3.51 (s, 3H), 3.28 (s, 3H) ppm 94 N-(2-((4-(3- 523.3 1H NMR (400 MHz, DMSO) δ = 12.31 (br s, (methylsulfonamidomethyl)phenyl)thiazol- 1H), 9.25 (br s, 1H), 8.45 (s, 1H), 8.24 (d, 2-yl)amino)-2-oxoethyl)-3- J = 7.2 Hz, 1H), 8.12 (d, J = 7.2 Hz, 1H), 7.92 (methylsulfonyl)benzamide (s, 1H), 7.81 (d, J = 7.6 Hz, 2H), 7.62 (s, 1H), 7.58 (br s, 1H), 7.41-7.40 (m, 1H), 7.30 (d, J = 7.2 Hz, 1H), 4.23 (d, J = 5.2 Hz, 2H), 4.20 (d, J = 4.8 Hz, 2H), 3.28 (s, 3H), 2.89 (s, 3H) ppm 95 N-(2-oxo-2-((4-(3-(pyridin-4- 456.2 1H NMR (400 MHz, DMSO) δ = 12.39 (s, yl)phenyl)thiazol-2- 1H), 8.88 (d, J = 6.4 Hz, 2H), yl)amino)ethyl)indoline-5- 8.48-8.47 (m, 1H), 8.42 (s, 1H), 8.18 (br d, carboxamide J = 6.0 Hz, 2H), 8.11 (d, J = 8.0 Hz, 1H), 7.94-7.84 (m, 2H), 7.68-7.67 (m, 1H), 7.63-7.53 (m, 2H), 6.53 (d, J = 8.0 Hz, 1H), 4.15-4.13 (m, 2H), 3.02-2.96 (m, 1H), 2.98 (m, 2H), 2.55 (br s, 2H) ppm 96 N-(2-oxo-2-((4-(3-(pyridin-4- 455.2 1H NMR (400 MHz, DMSO) δ = 13.37 (s, yl)phenyl)thiazol-2- 1H), 12.54 (s, 1H), 9.00 (s, 1H), yl)amino)ethyl)-1H-indazole- 8.75-8.74 (m, 2H), 8.47 (s, 1H), 8.37 (s, 5-carboxamide 1H), 8.31 (s, 1H), 8.09-8.07 (m, 1H), 7.91-7.90 (m, 1H), 7.85 (s, 1H), 7.83-7.82 (m, 3H), 7.69-7.66 (m, 2H), 4.30- 4.28 (m, 2H) ppm 97 (S)-N-(4-(methylthio)-1-oxo-1-((4-(3- 489.2 1H NMR (400 MHz, DMSO) δ = 12.55 (s, (pyridin-4-yl)phenyl)thiazol- 1H), 8.77 (d, J = 7.2 Hz, 1H), 2-yl)amino)butan-2- 8.71-8.65 (m, 2H), 8.32 (s, 1H), 8.02 (d, J = yl)benzamide 8.0 Hz, 1H), 7.97-7.92 (m, 2H), 7.85 (s, 1H), 7.80-7.75 (m, 3H), 7.63-7.55 (m, 2H), 7.54-7.47 (m, 1H), 7.54-7.47 (m, 1H), 4.80- 4.75 (m, 1H), 2.60-2.57 (m, 2H), 2.18-2.12 (m, 2H), 2.11 (s, 3H) ppm 98 3-(methylsulfonyl)-N-(2-oxo-2-((4-(3- 498.9 1H NMR (400 MHz, DMSO) δ = 12.57 (s, (thiazol-2-yl)phenyl)thiazol-2- 1H), 9.28-9.25 (m, 1H), yl)amino)ethyl)benzamide 8.55-8.54 (m, 1H), 8.45 (d, J = 1.6 Hz, 1H), 8.24 (d, J = 8.0 Hz, 1H), 8.12 (d, J = 8.4 Hz, 1H), 8.01 (d, J = 8.0 Hz, 1H), 7.96 (d, J = 3.2 Hz, 1H), 7.92-7.90 (m, 1H), 7.82-7.79 (m, 3H), 7.59-7.55 (m, 1H), 4.25 (d, J = 6.0 Hz, 2H), 3.28 (s, 3H) ppm 99 N-(2-oxo-2-((4-(3-(pyridin-4- 470.3 1H NMR (400 MHz, DMSO) δ = 12.36 (br s, yl)phenyl)thiazol-2-yl)amino)ethyl)- 1H), 8.73-8.64 (m, 2H), 8.38 (s, 1H), 8.32- 1,2,3,4-tetrahydroquinoline-6- 8.31 (m, 1H), 8.01 (d, J = 7.8 Hz, 1H), 7.82 carboxamide (s, 1H), 7.79-7.73 (m, 3H), 7.60 (m, 1H), 7.49-7.40 (m, 2H), 6.44 (d, J = 9.0 Hz, 1H), 6.29 (s, 1H), 4.12 (d, J = 5.8 Hz, 2H), 3.23 (br s, 2H), 2.71-2.70 (m, 2H), 1.89-1.75 (m, 2H) ppm 100 4-(aminomethyl)-N-(2-oxo-2-((4-(3- 444 1H NMR (400 MHz, DEUTERIUM OXIDE) (pyridin-4-yl)phenyl)thiazol- δ = 8.61 (d, J = 6.8 Hz, 2H), 2-yl)amino)ethyl)benzamide 8.08 (d, J = 6.8 Hz, 2H), 7.85 (d, J = 8.4 Hz, 2H), 7.81 (s, 1H), 7.71-7.69 (m, 1H), 7.59-7.57 (m, 1H), 7.56 (d, J = 8.4 Hz, 2H), 7.43-7.41 (m, 1H), 7.27 (s, 1H), 4.30 (s, 2H), 4.24 (s, 2H) ppm 101 N-(2-oxo-2-((4-(3-(pyridin-4- 455 1H NMR (400 MHz, DMSO) δ = 12.24 (br s, yl)phenyl)thiazol-2- 1H), 9.03-8.95 (m, 2H), yl)amino)ethyl)imidazo[1,5- 8.71-8.65 (m, 2H), 8.55 (s, 1H), 8.31 (s, a]pyridine-6-carboxamide 1H), 8.02 (d, J = 7.8 Hz, 1H), 7.84 (s, 1H), 7.79-7.73 (m, 3H), 7.66-7.56 (m, 2H), 7.43 (s, 1H), 7.22-7.21 (m, 1H), 4.23 (d, J = 5.8 Hz, 2H) ppm 102 N-(2-oxo-2-((4-(3-(pyridin-4- 471.1 1H NMR (400 MHz, DMSO) δ = 8.63 (d, yl)phenyl)thiazol-2-yl)amino)ethyl)- J = 5.8 Hz, 2H), 8.28-8.25 (m, 2H), 5,6,7,8-tetrahydro-1,8-naphthyridine- 7.98 (d, J = 7.8 Hz, 1H), 7.75-7.72 (m, 4H), 3-carboxamide 7.60-7.58 (m, 2H), 4.11 (s, 2H), 3.30-3.27 (m, 2H), 2.68-2.67 (m, 2H), 1.77-1.74 (m, 2H) ppm 103 4-(3-hydroxyazetidin-1-yl)-N-(2-oxo-2- 486.1 1H NMR (400 MHz, DMSO) δ = 12.36 (br s, ((4-(3-(pyridin-4-yl)\phenyl)thiazol-2- 1H), 8.68 (d, J = 4.8 Hz, 2H), yl)amino)ethyl)benzamide 8.53 (br s, 1H), 8.31 (s, 1H), 8.02 (d, J = 7.2 Hz, 1H), 7.80-7.74 (m, 6H), 7.64-7.56 (m, 1H), 6.45 (d, J = 8.0 Hz, 2H), 5.69 (d, J = 6.4 Hz, 1H), 4.60 (d, J = 6.0 Hz, 1H), 4.18-4.10 (m, 4H), 3.62-3.59 (m, 2H) ppm 104 4-methylsulfonyl-N-[2-oxo-2-[[4-[3-(4- 494.1 1H NMR (400 MHz, DMSO) δ = 12.51 (s, pyridyl)phenyl]thiazol-2- 1H), 9.32-9.29 (m, 1H), 9.06 (d, J = 4.8 Hz, yl]amino]ethyl]pyridine-2-carboxamide 1H), 8.80 (d, J = 5.6 Hz, 2H), 8.44 (s, 1H), 8.37 (s, 1H), 8.17-8.16 (m, 1H), 8.07-8.01 (m, 3H), 7.86-7.83 (m, 2H), 7.66-7.62 (m, 1H), 4.31 (d, J = 6.0 Hz, 2H), 3.41 (s, 3H) ppm 105 3-hydroxy-N-[2-oxo-2-[[4-[3-(4- 486.3 1H NMR (400 MHz, DMSO) δ = 8.87 (d, pyridyl)phenyl]thiazol-2-yl]amino]ethyl]- J = 4.8 Hz, 2H), 8.46-8.35 (m, 2H), 8.21- 1,2,3,4-tetrahydroquinoline-6-carboxamide 8.06 (m, 3H), 7.92-7.83 (m, 2H), 7.67-6.64 (m, 1H), 7.51-7.43 (m, 2H), 6.45 (d, J = 9.0 Hz, 1H), 4.13 (d, J = 5.7 Hz, 2H), 4.01-3.92 (m, 1H), 3.33-3.25 (m, 1H), 2.99-2.97 (m, 1H), 2.87 (d, J = 13.0 Hz, 1H), 2.69-2.65 (m, 1H), 2.61 (d, J = 7.6 Hz, 2H), 2.44-2.43 (m, 1H) ppm 106 4-amino-N-(2-oxo-2-((4-phenylthiazol- 353.1 1H NMR (400 MHz, DMSO-d6) δ = 2-yl)amino)ethyl)benzamide 12.32 (br s, 1H), 8.42-8.39 (m, 1H), 7.90 (d, J = 7.6 Hz, 2H), 7.62-7.60 (m, 3H), 7.44-7.41 (m, 2H), 7.34-7.30 (m, 1H), 6.57 (d, J = 7.6 Hz, 2H), 5.65 (s, 2H), 4.12 (d, J = 5.2 Hz, 2H) ppm. 107 (S)-4-amino-N-(1-((4-(4- 452.1 1H NMR (400 MHz, DMSO-d6) δ = 12.44 cyanophenyl)thiazol-2-yl)amino)- (s, 1H), 8.23 (d, J = 7.2 Hz, 1H), 4-(methylthio)-1-oxobutan-2- 8.08 (d, J = 8.4 Hz, 2H), 7.92-7.88 (m, 3H), yl)benzamide 7.65 (d, J = 8.0 Hz, 2H), 6.58 (d, J = 8.4 Hz, 2H), 4.72-4.67 (m, 1H), 2.64- 2.60 (m, 2H), 2.08 (s, 5H) ppm 108 4-amino-N-(2-((4-(4- 383.1 1HNMR (400 MHz, DMSO-d6) δ =12.28 (br methoxyphenyl)thiazol-2-yl)amino)- s, 1H), 8.44-8.43 (m, 1H), 2-oxoethyl)benzamide 7.84-7.82 (m, J = 8.8 Hz, 2H), 7.62-7.60 (m, 2H), 7.44 (s, 1H), 7.00-6.98 (m, 2H), 6.57-6.55 (m, 2H), 5.66 (s, 2H), 4.11 (d, J = 5.6 Hz, 2H), 3.79 (s, 3H) ppm 109 4-amino-N-(2-((4-(2,3- 411.3 1H NMR (400 MHz, DMSO-d6) δ = 12.27 dihydrobenzo[b][1,4]dioxin-6- (br s, 1H), 8.40-8.38 (m, 1H), yl)thiazol-2-yl)amino)-2- 7.61 (d, J = 8.8 Hz, 2H), 7.45 (s, 1H), 7.41- oxoethyl)benzamide 7.34 (m, 2H), 6.89 (d, J = 8.0 Hz, 1H), 6.56 (d, J = 8.8 Hz, 2H), 5.65 (s, 2H), 4.26 (s, 4H), 4.10 (d, J = 6.0 Hz, 2H) ppm 110 methyl 3-(2-(2-(4- 416.1 1H NMR (400 MHz, MeOD) δ = 7.67 (d, aminobenzamido)acetamido)thiazol-4- J = 8.6 Hz, 2H), 6.92 (s, 1H), yl)-5,6-dihydropyridine-1(2H)-carboxylate 6.69 (d, J = 8.6 Hz, 2H), 6.66 (s, 1H), 4.30 (s, 2H), 4.22 (s, 2H), 3.73 (s, 3H), 3.60-3.58 (m, 2H), 2.32-2.31 (m, 2H) ppm 111 4-amino-N-(2-((4-(2,3- 395.3 1H NMR (400 MHz, DMSO-d6) δ = 12.24 (s, dihydrobenzofuran-5- 1H), 8.40 (s, 1H), 7.75 (s, 1H), yl)thiazol-2-yl)amino)- 7.69-7.56 (m, 3H), 7.38 (s, 1H), 6.80 (d, 2-oxoethyl)benzamide J = 8.4 Hz, 1H), 6.56 (d, J = 8.8 Hz, 2H), 5.65 (s, 2H), 4.56 (m, 2H), 4.10 (d, J = 5.6 Hz, 2H), 3.26-3.16 (m, 2H) ppm 112 4-amino-N-(2-((4-(2,3- 395.2 1H NMR (400 MHz, DMSO-d6) δ = 12.47- dihydrobenzofuran-6- 11.92 (m, 1H), 8.30 (br s, 1H), yl)thiazol-2-yl)amino)-2- 7.60 (d, J = 8.4 Hz, 2H), 7.46 (br s, 1H), oxoethyl)benzamide 7.38 (s, 1H), 7.27 (s, 1H), 7.24 (d, J = 7.6 Hz, 1H), 6.56 (d, J = 8.4 Hz, 2H), 5.64 (s, 2H), 4.55 (m, 2H), 4.06 (d, J = 5.6 Hz, 2H), 3.18 (m, 2H) ppm 113 4-amino-N-(2-((4-(3-(5-methyl-1,3,4- 435.1 1H NMR (400 MHz, DMSO-d6) δ = 12.47 oxadiazol-2-yl)phenyl)thiazol- (s, 1H), 8.54 (s, 1H), 8.46-8.43 (m, 1H), 2-yl)amino)-2- 8.13 (br d, J = 8.0 Hz, 1H), 7.93 (d, J = 8.0 oxoethyl)benzamide Hz, 1H), 7.84-7.83 (m, 1H), 7.67-7.60 (m, 3H), 6.56 (d, J = 8.8 Hz, 2H), 5.68 (s, 2H), 4.12 (d, J = 5.6 Hz, 2H), 2.60 (s, 3H) ppm 114 4-amino-N-(2-((4-(2- 369.3 1H NMR (400 MHz, DMSO-d6) δ = 12.40 hydroxyphenyl)thiazol-2-yl)amino)- (s, 1H), 10.90 (s, 1H), 8.47 (s, 1H), 2-oxoethyl)benzamide 7.92-7.91 (m, 1H), 7.72 (s, 1H), 7.62 (d, J = 8.6 Hz, 2H), 7.22-7.13 (m, 1H), 6.96-6.85 (m, 2H), 6.57 (d, J = 8.6 Hz, 2H), 5.68 (br s, 2H), 4.13 (d, J = 5.8 Hz, 2H) ppm 115 4-amino-N-(2-((4-(4- 369.1 1HNMR (400 MHz, DMSO-d6) δ = 12.22 hydroxyphenyl)thiazol-2- (br s, 1H), 9.81-9.42 (m, 1H), 8.41 (br s, yl)amino)-2-oxoethyl)benzamide 1H), 7.78-7.51 (m, 4H), 7.34 (s, 1H), 6.81 (br d, J = 8.8 Hz, 2H), 6.57 (br d, J = 8.4 Hz, 2H), 5.66 (br s, 2H), 4.11 (br d, J = 5.2 Hz, 2H) ppm 116 (S)-4-amino-N-(1-((4-(5-cyano-[1,1′- 528.3 1H NMR (400 MHz, CDCI3) δ = 11.10 (br s, biphenyl]-3-yl)thiazol-2-yl)amino)- 1H), 8.29 (s, 1H), 8.14 (s, 1H), 4-(methylthio)-1-oxobutan-2- 7.83-7.80 (m, 3H), 7.66-7.64 (m, 2H), 7.53- yl)benzamide 7.50 (m, 2H), 7.47-7.43 (m, 1H), 7.31 (s, 1H), 7.12 (d, J = 8.0 Hz, 1H), 6.72 (d, J = 8.0 Hz, 2H), 5.31-5.26 (m, 1H), 2.81-2.69 (m, 2H), 2.44- 2.39 (m, 1H), 2.35-2.28 (m, 1H), 2.18 (s, 3H) ppm 117 4-amino-N-(2-oxo-2-((4-(3-(pyridin-4- 430.0 1H NMR (400 MHz, MeOD) δ = 8.89 (d, yl)phenyl)thiazol-2- J = 6.8 Hz, 2H), 8.51 (s, 1H), 8.43 (d, J = 6.8 yl)amino)ethyl)benzamide Hz, 2H), 8.21 (d, J = 7.6 Hz, 1H), 7.92 (br d, J = 8.0 Hz, 1H), 7.75 (d, J = 8.8 Hz, 2H), 7.71-7.67 (m, 1H), 7.64 (s, 1H), 6.81 (d, J = 8.8 Hz, 2H), 4.30 (s, 2H) ppm 118 (S)-4-amino-N-(1-((4-(4′- 532.4 1H NMR (400 MHz, MeOD) δ = 8.19 (s, (aminomethyl)-[1,1′-biphenyl]-3- 1H), 7.91 (d, J = 7.6 Hz, 1H), yl)thiazol-2-yl)amino)-4-(methylthio)- 7.79-7.75 (m, 4H), 7.59-7.54 (m, 3H), 7.51- 1-oxobutan-2-yl)benzamide 7.48 (m, 2H), 6.84 (br d, J = 8.4 Hz, 2H), 4.89 (br s, 1H), 4.17 (s, 2H), 2.73-2.59 (m, 2H), 2.32-2.17 (m, 2H), 2.13 (br s, 3H) ppm 119 (S)-4-amino-N-(1-((4-(6-cyano-[1,1′- 528.2 1H NMR (400 MHz, DMSO-d6) δ = 12.43 biphenyl]-3-yl)thiazol-2-yl)amino)- (s, 1H), 8.19 (s, 1H), 8.14 (s, 1H), 4-(methylthio)-1-oxobutan-2- 8.09 (d, J = 8.4 Hz, 1H), 8.04 (d, J = 8.0 Hz, yl)benzamide 2H), 7.64 (d, J = 7.2 Hz, 4H), 7.58-7.51 (m, 3H), 6.55 (d, J = 8.8 Hz, 2H), 5.66 (s, 2H), 4.66 (s, 1H), 2.64-2.61 (m, 2H), 2.07 (m, 5H) ppm 120 (S)-N-(1-((4-(3- 498.1 1H NMR (400 MHz, DMSO-d6) δ = 12.31 (acetamidomethyl)phenyl)thiazol- (s, 1H), 8.38-8.34 (m, 1H), 2-yl)amino)-4-(methylthio)-1- 8.21 (br d, J = 7.2 Hz, 1H), 7.79 (s, 1H), oxobutan-2-yl)-4- 7.76 (d, J = 7.6 Hz, 1H), 7.64 (d, J = 8.8 Hz, aminobenzamide 2H), 7.59 (s, 1H), 7.39-7.35 (m, 1H), 7.20 (d, J = 7.2 Hz, 1H), 6.55 (d, J = 8.4 Hz, 2H), 5.67 (s, 2H), 4.67 (br d, J = 7.2 Hz, 1H), 4.28 (d, J = 6.0 Hz, 2H), 2.64-2.61 (m, 2H), 2.08-2.05 (m, 5H), 1.88 (s, 3H) ppm 121 (S)-N-(1-((4-(4- 498.1 1H NMR (400 MHz, DMSO) δ = 12.36 (s, (acetamidomethyl)phenyl)thiazol- 1H), 8.35-8.32 (m, 1H), 8.21 (d, J = 8.0 Hz, 2-yl)amino)-4-(methylthio)-1- 1H), 7.83 (d, J = 8.4 Hz, 2H), 7.66-7.63 (m, oxobutan-2-yl)-4- 2H), 7.57 (s, 1H), 7.29 (d, J = 8.4 Hz, 2H), aminobenzamide 6.57 (d, J = 8.4 Hz, 2H), 4.70-4.65 (m, 1H), 4.26 (d, J = 5.6 Hz, 2H), 2.64-2.50 (m, 2H), 2.08-2.04 (m, 5H), 1.87 (s, 3H) ppm 122 (S)-methyl 3-(2-(2-(4- 418.1 1H NMR (400 MHz, MeOD) δ = 7.67-7.65 aminobenzamido)acetamido)thiazol- (m, 2H), 6.76 (s, 1H), 6.69-6.67 (m, 2H), 4-yl)piperidine-1-carboxylate 4.25 (br d, J = 14.0 Hz, 1H), 4.21 (s, 2H), 4.03 (br d, J = 10.4 Hz, 1H), 3.68 (s, 3H), 2.98-2.92 (m, 2H), 2.78-2.76 (m, 1H), 2.10-2.07 (m, 1H), 1.77-1.74 (m, 2H), 1.71-1.56 (m, 1H) ppm 123 N-(2-((4-(4′-(acetamidomethyl)- 500.1 1H NMR (400 MHz, DMSO) δ = 12.36 (s, [1,1′-biphenyl]-3-yl)thiazol-2- 1H), 8.42-8.35 (m, 2H), 8.18 (s, 1H), 7.89 yl)amino)-2-oxoethyl)-4- (d, J = 7.6 Hz, 1H), 7.75 (s, 1H), 7.67 (d, aminobenzamide J = 8.0 Hz, 2H), 7.62-7.59 (m, 3H), 7.53- 7.49 (m, 1H), 7.38 (d, J = 8.0 Hz, 2H), 6.57 (d, J = 8.8 Hz, 2H), 5.66 (s, 2H), 4.31 (d, J = 6.0 Hz, 2H), 4.12 (d, J = 6.0 Hz, 2H), 1.90 (s, 3H) ppm 124 N-(2-((4-(3′-(acetamidomethyl)-[1,1′- 500.3 1H NMR (400 MHz, MeOD) δ = 8.20-8.19 biphenyl]-3-yl)thiazol-2-yl)amino)- (m, 1H), 7.90 (d, J = 7.6 Hz, 1H), 2-oxoethyl)-4-aminobenzamide 7.86-7.77 (m, 2H), 7.66-7.56 (m, 3H), 7.53- 7.43 (m, 3H), 7.32 (d, J = 8.0 Hz, 1H), 6.97 (d, J = 8.4 Hz, 2H), 4.46 (s, 2H), 4.31 (s, 2H), 2.03 (s, 3H) ppm 125 (S)-4-amino-N-(1-oxo-1-((4-(3-(pyridin- 444.1 H NMR (400 MHz, DMSO-d6) δ = 12.33 (br 4-yl)phenyl)thiazol-2-yl)amino)propan-2- s, 1H), 8.68-8.66 (m, 2H), 8.30 (s, 1H), yl)benzamide 8.20 (br d, J = 6.4 Hz, 1H), 8.01 (br d, J = 8.0 Hz, 1H), 7.82 (s, 1H), 7.76-7.74 (m, 3H), 7.65 (d, J = 8.8 Hz, 2H), 7.61-7.57 (m, 1H), 6.56 (d, J = 8.4 Hz, 2H), 5.65 (s, 2H), 4.67- 4.61 (m, 1H), 1.43 (d, J = 7.2 Hz, 3H) ppm 126 4-(methylamino)-N-(2-oxo-2-((4-(3- 444.3 1H NMR (400 MHz, DMSO-d6) δ = 12.24- (pyridin-4-yl)phenyl)thiazol-2- 12.23 (m, 1H), 8.67 (d, J = 6.4 Hz, 2H), yl)amino)ethyl)benzamide 8.47-8.44 (m, 1H), 8.30 (s, 1H), 8.01 (br d, J = 7.6 Hz, 1H), 7.82 (s, 1H), 7.76 (br d, J = 6.0 Hz, 3H), 7.69 (d, J = 8.8 Hz, 2H), 7.61-7.57 (m, 1H), 6.55 (d, J = 8.8 Hz, 2H), 6.25-6.22 (m, 1H), 4.13 (br d, J = 6.0 Hz, 2H), 2.72 (d, J = 4.8 Hz, 3H) ppm 127 4-amino-N-(2-((4-(3-(6-methoxypyridin- 460.0 1H NMR (400 MHz, DMSO) δ = 12.34 (br s, 3-yl)phenyl)thiazol-2-yl)amino)-2- 1H), 8.54 (d, J = 2.4 Hz, 1H), oxoethyl)benzamide 8.41 (br s, 1H), 8.16 (s, 1H), 8.07-8.05 (m, 1H), 7.90 (d, J = 8.0 Hz, 1H), 7.77 (s, 1H), 7.61 (d, J = 8.4 Hz, 3H), 7.54- 7.50 (m, 1H), 6.95 (d, J = 8.4 Hz, 1H), 6.56 (d, J = 8.4 Hz, 2H), 5.65 (s, 2H), 4.12 (br d, J = 5.6 Hz, 2H), 3.91 (s, 3H) ppm 128 (S)-N-(1-((4-(3-(1,3,4-oxadiazol-2- 495.3 1H NMR (400 MHz, DMSO-d6) δ = 12.46 yl)phenyl)thiazol-2-yl)amino)- (s, 1H), 9.37 (s, 1H), 8.61 (s, 1H), 4-(methylthio)-1-oxobutan-2-yl)-4- 8.21 (d, J = 6.8 Hz, 1H), 8.15 (d, J = 8.4 Hz, aminobenzamide 1H), 7.98 (d, J = 7.6 Hz, 1H), 7.84 (s, 1H), 7.69-7.64 (m, 3H), 6.56 (d, J = 8.8 Hz, 2H), 5.66 (s, 2H), 4.71-4.67 (m, 1H), 2.64-2.54 (m, 2H), 2.12-2.09 (m, 5H) ppm 129 4-amino-N-(2-((4-(3′-((N- 514.3 1H NMR (400 MHz, DMSO) δ = 12.37 (br s, methylacetamido)methyl)-[1,1′- 1H), 8.43 (br s, 1H), 8.17 (br s, 1H), 7.91 biphenyl]-3-yl)thiazol-2-yl)amino)-2- (br d, J = 7.2 Hz, 1H), 7.76 (s, 1H), 7.65- oxoethyl)benzamide 7.32 (m, 7H), 7.28-7.19 (m, 1H), 6.57 (d, J = 8.4 Hz, 2H), 5.67 (s, 2H), 4.69-4.54 (m, 2H), 4.13 (m, 2H), 3.02-2.82 (m, 3H), 2.12- 2.03 (m, 3H) ppm 130 3′-(2-(2-(4- 486.1 1H NMR (400 MHz, DMSO) δ = 12.37 (s, aminobenzamido)acetamido)thiazol- 1H), 8.50-8.49 (m, 1H), 4-yl)-N-methyl-[1,1′-biphenyl]-4- 8.44-8.41 (m, 1H), 8.25 (s, 1H), 8.00-7.92 carboxamide (m, 3H), 7.85-7.79 (m, 3H), 7.69 (d, J = 8.2 Hz, 1H), 7.62 (d, J = 8.6 Hz, 2H), 7.59-7.53 (m, 1H), 6.57 (d, J = 8.6 Hz, 2H), 5.67 (s, 2H), 4.13 (d, J = 5.6 Hz, 1H), 4.16-4.11 (m, 1H), 2.82 (d, J = 4.6 Hz, 3H) ppm 131 3′-(2-(2-(4- 500.3 1H NMR (400 MHz, DMSO) δ = 12.38 (s, aminobenzamido)acetamido)thiazol- 1H), 8.44-8.41 (m, 1H), 8.24 (s, 1H), 7.94 4-yl)-N,N-dimethyl-[1,1′-biphenyl]-3- (d, J = 7.8 Hz, 1H), 7.83-7.78 (m, 2H), 7.72 carboxamide (s, 1H), 7.67 (d, J = 7.8 Hz, 1H), 7.62 (d, J = 8.6 Hz, 2H), 7.59-7.53 (m, 2H), 7.42 (d, J = 7.6 Hz, 1H), 6.57 (d, J = 8.6 Hz, 2H), 5.67 (br s, 2H), 4.13 (d, J = 5.6 Hz, 2H), 3.08-2.91 (m, 6H) ppm 132 4-(dimethylamino)-N-(2-oxo-2-((4-(3- 458.1 1H NMR (400 MHz, DMSO-d6) δ = 12.40 (pyridin-4-yl)phenyl)thiazol-2- (s, 1H), 8.82 (d, J = 6.4 Hz, 2H), yl)amino)ethyl)benzamide 8.56-8.53 (m, 1H), 8.38 (s, 1H), 8.07 (br d, J = 6.4 Hz, 3H), 7.85 (s, 2H), 7.76 (d, J = 8.8 Hz, 2H), 7.66- 7.63 (m, 1H), 6.73 (d, J = 8.8 Hz, 2H), 4.15 (d, J = 5.6 Hz, 2H), 2.98 (s, 6H) ppm 133 4-amino-N-(2-((4-(3-(2-aminopyridin-4- 445 1H NMR (400 MHz, DMSO) δ = 12.38 (br s, yl)phenyl)thiazol-2-yl)amino)-2- 1H), 8.44-8.43 (m, 1H), oxoethyl)benzamide 8.23-8.13 (m, 2H), 8.04-7.92 (m, 2H), 7.76 (s, 1H), 7.68-7.51 (m, 4H), 6.82 (m, 1H), 6.75 (s, 1H), 6.57 (d, J = 8.8 Hz, 2H), 6.00 (s, 2H), 5.67 (br s, 1H), 4.13 (d, J = 5.6 Hz, 2H) ppm 134 4-amino-N-(2-((4-(4′- 507.2 1H NMR (400 MHz, DMSO) δ = 8.43 (s, (methylsulfonyl)- 1H), 8.27 (s, 1H), 8.08-7.96 (m, 5H), 7.81 [1,1′-biphenyl]-3- (s, 1H), 7.72 (br d, J = 7.6 Hz, 1H), 7.67- yl)thiazol-2-yl)amino)-2- 7.54 (m, 3H), 6.57 (d, J = 8.6 Hz, 2H), 5.71- oxoethyl)benzamide 5.63 (m, 1H), 5.67 (s, 1H), 4.13 (br d, J = 5.6 Hz, 2H), 3.28 (s, 3H) ppm 135 4-amino-N-(2-((4-(3′-(aminomethyl)- 458.3 1H NMR (400 MHz, DMSO) δ = 12.35 (s, [1,1′-biphenyl]-3-yl)thiazol-2- 1H), 8.47-8.46 (m, 1H), 8.21 (s, 4H), 7.94 yl)amino)-2-oxoethyl)benzamide (d, J = 7.6 Hz, 1H), 7.86 (s, 1H), 7.79-7.73 (m, 2H), 7.42-7.69 (m, 7H), 6.59 (d, J = 8.8 Hz, 2H), 4.18-4.11 (m, 4H) ppm 136 3′-(2-(2-(4- 473.1 1H NMR (400 MHz, DMSO) δ = 13.12 (br aminobenzamido)acetamido)thiazol- s, 1H), 12.40 (s, 1H), 4-yl)-[1,1′-biphenyl]- 8.43 (br s, 1H), 8.25 (br s, 2H) 8.04-7.92 3-carboxylic acid (m, 3H), 7.80 (s, 1H), 7.72-7.53 (m, 5H), 6.57 (br d, J = 8.2 Hz, 2 H), 5.67 (br s, 2H), 4.20-4.08 (m, 1H), 4.13 (br d, J = 5.2 Hz, 1H) ppm 137 3′-(2-(2-(4- 500.1 1H NMR (400 MHz, DMSO) δ = 12.37 (s, aminobenzamido)acetamido)thiazol- 1H), 8.44-8.41 (m, 1H), 8.24 (s, 1H), 7.96- 4-yl)-N,N-dimethyl-[1,1′- 7.92 (m, 1H), 7.79 (d, J = 6.6 Hz, 3H), 7.67 biphenyl]-4-carboxamide (d, J = 8.2 Hz, 1H), 7.62 (d, J = 8.6 Hz, 2H), 7.58 (s, 1H), 7.59- 7.52 (m, 2H), 6.57 (d, J = 8.6 Hz, 2H), 5.67 (s, 2H), 4.13 (d, J = 5.8 Hz, 2H), 2.99 (br s, 6H) ppm 138 4-amino-N-(2-((4-(4′-((N- 514.1 1H NMR (400 MHz, DMSO) δ = 12.38 (s, methylacetamido)methyl)- 1H), 8.47-8.44 (m, 1H), [1,1′-biphenyl]-3-yl)thiazol-2- 8.19-8.17 (m, 1H), 7.90-7.88 (m, 1H), 7.77 yl)amino)-2-oxoethyl)benzamide (d, J = 2.4 Hz, 1H), 7.74-7.66 (m, 2H), 7.63-7.59 (m, 3H), 7.54- 7.49 (m, 1H), 7.35-7.32 (m, 2H), 6.60 (d, J = 8.4 Hz, 2H), 4.61-4.54 (m, 2H), 4.13 (d, J = 5.6 Hz, 2H), 2.94-2.83 (m, 3H), 2.08-2.07 (m, 3H) ppm 139 4-amino-N-(2-((4-(4′-hydroxy- 445.2 1H NMR (400 MHz, DMSO) δ = 12.35 (s, [1,1′-biphenyl]-3-yl)thiazol-2- 1H), 9.57 (s, 1H), yl)amino)-2-oxoethyl)benzamide 8.43-8.41 (m, 1H), 8.10 (s, 1H), 7.80 (d, J = 7.6 Hz, 1H), 7.72 (s, 1H), 7.61 (d, J = 8.8 Hz, 2H), 7.54- 7.51 (m, 3H), 7.47-7.43 (m, 1H), 6.88-6.86 (m, 2H), 6.55 (d, J = 8.4 Hz, 2H), 5.6 (s, 2H), 4.11 (d, J = 5.6 Hz, 2H) ppm 140 4-amino-N-(2-((4-(3-(1-methyl-2-oxo- 460.2 1H NMR (400 MHz, DMSO) δ = 12.48- 1,2-dihydropyridin-4- 12.23 (m, 1H), 8.43 (br s, 1H), 8.24 (s, yl)phenyl)thiazol-2-yl)amino)-2- 1H), 8.00 (d, J = 8.2 Hz, 1H), 7.86-7.79 (m, oxoethyl)benzamide 2H), 7.67 (d, J = 7.6 Hz, 1H), 7.62 (d, J = 8.6 Hz, 2H), 7.58-7.52 (m, 1H), 6.75 (d, J = 2.2 Hz, 1H), 6.63 (dd, J = 2.2, 7.2 Hz, 1H), 6.57 (d, J = 8.8 Hz, 2H), 5.68 (s, 2H), 4.12 (d, J = 5.8 Hz, 2H), 3.47 (s, 3H) ppm 141 4-amino-N-(2-((4-(3′-hydroxy-[1,1′- 445.2 1H NMR (400 MHz, DMSO) δ = 12.37 (br s, biphenyl]-3-yl)thiazol-2- 1H), 9.56 (s, 1H), yl)amino)-2-oxoethyl)benzamide 8.45-8.43 (m, 1H), 8.15 (s, 1H), 7.89 (d, J = 7.6 Hz, 1H), 7.75 (s, 1H), 7.62 (d, J = 8.4 Hz, 2H), 7.56- 7.48 (m, 2H), 7.31-7.27 (m, 1H), 7.12 (d, J = 8.4 Hz, 1H), 7.08-7.06 (m, 1H), 6.80 (dd, J = 2.0, 7.6 Hz, 1H), 6.57 (d, J = 8.8 Hz, 2H), 5.67 (s, 2H), 4.12 (d, J = 6.0 Hz, 2H) ppm 142 4-amino-N-(2-((4-(2′-fluoro- 446.9 1H NMR (400 MHz, DMSO) δ = 12.26 (br s, [1,1′-biphenyl]-3-yl)thiazol-2- 1H), 8.43-8.42 (m, 1H), yl)\amino)-2-oxoethyl)benzamide 8.10 (d, J = 1.2 Hz, 1H), 7.94-7.93 (m, 1H), 7.72 (s, 1H), 7.62-7.51 (m, 6H), 7.34-7.33 (m, 2H), 6.57 (d, J = 8.8 Hz, 2H), 5.65 (s, 2H), 4.12 (d, J = 6.0 Hz, 2H) ppm 143 4-amino-N-(2-((4-(2-methyl-1-oxo-1,2- 434.1 1H NMR (400 MHz, DMSO-d6) δ = 12.43 dihydroisoquinolin-7-yl)thiazol-2- (br s, 1H), 8.81 (s, 1H), 8.40 (br s, 1H), yl)amino)-2-oxoethyl)benzamide 8.21-8.19 (m, 1H), 7.74 (br s, 1H), 7.70 (d, J = 8.0 Hz, 1H), 7.61 (d, J = 8.4 Hz, 2H), 7.48 (d, J = 7.2 Hz, 1H), 6.63 (d, J = 7.2 Hz, 1H), 6.57 (d, J = 8.4 Hz, 2H), 5.65 (s, 2H), 4.12 (br d, J = 5.6 Hz, 2H), 3.52 (s, 3H) ppm 144 3-[3-[2-[[2-[(4- 486.4 1H NMR (400 MHz, DMSO) δ = 12.42 (s, aminobenzoyl)amino]acetyl]amino]thiazol- 1H), 8.58 (br d, J = 4.6 Hz, 1H), 8.48- 4-yl]phenyl]-N-methyl-benzamide 8.47 (m, 1H), 8.26 (s, 1H), 8.17 (s, 1 H), 7.94 (d, J = 7.8 Hz, 1H), 7.90-7.84 (m, 2H), 7.78 (s, 1H), 7.70 (br d, J = 8.2 Hz, 1H), 7.66 (d, J = 8.6 Hz, 2H), 7.59- 7.57 (m, 2H), 6.64 (d, J = 8.7 Hz, 2H), 4.14 (d, J = 5.6 Hz, 2H), 2.84 (d, J = 4.6 Hz, 3H) ppm 145 4-amino-N-(2-((4-(4′- 486.1 1H NMR (400 MHz, MeOD) δ = 8.18 (s, ((dimethylamino)methyl)-[1,1′- 1H), 7.87 (d, J = 8.0 Hz, 1H), biphenyl]-3-yl)thiazol-2- 7.69-7.65 (m, 4H), 7.57 (d, J = 8.0 Hz, 1H), yl)amino)-2-oxoethyl)benzamide 7.49-7.40 (m, 4H), 6.70-6.68 (m, 2H), 4.27 (s, 2H), 3.54 (s, 2H), 2.28 (s, 6H) ppm 146 4-amino-N-(2-((4-(3-(2-methoxypyridin- 460.2 1H NMR (400 MHz, DMSO) δ = 12.37 (s, 4-yl)phenyl)thiazol-2-yl)amino)- 1H), 8.44 (br s, 1H), 2-oxoethyl)benzamide 8.28-8.26 (m, 2H), 8.00 (d, J = 7.6 Hz, 1H), 7.82 (s, 1H), 7.73 (d, J = 8.0 Hz, 1H), 7.63 (br d, J = 6.8 Hz, 2H), 7.59-7.55 (m, 1H), 7.37-7.35 (m, 1H), 7.16 (s, 1H), 6.59 (br d, J = 4.8 Hz, 2H), 4.12 (br d, J = 5.2 Hz, 2H), 3.91 (s, 3H) ppm 147 4-amino-N-(2-oxo-2-((4-(1-oxo-1,2- 420.1 1H NMR (400 MHz, DMSO) δ = 12.43 (br s, dihydroisoquinolin-7-yl)thiazol-2- 1H), 11.24 (br d, J = 5.2 Hz, 1H), yl)amino)ethyl)benzamide 8.78 (s, 1H), 8.43-8.42 (m, 1H), 8.22-8.20 (m, 1H), 7.76 (s, 1H), 7.71 (d, J = 8.6 Hz, 1H), 7.63 (d, J = 8.6 Hz, 2H), 7.20-7.17 (m, 1H), 6.58-6.56 (m, 3H), 5.66 (s, 2H), 4.13 (d, J = 5.6 Hz, 2H) ppm 148 4-amino-N-(2-((4-(3-methyl-4-oxo-3,4- 435.3 1H NMR (400 MHz, DMSO-d6) δ = 12.31 dihydroquinazolin-6-yl)thiazol-2- (br s, 1H), 8.71 (s, 1H), yl)amino)-2-oxoethyl)benzamide 8.38-8.37 (m, 2H), 8.33-8.31 (m, 1H), 7.79 (s, 1H), 7.71 (d, J = 8.6 Hz, 1H), 7.61 (d, J = 8.6 Hz, 2H), 6.56 (d, J = 8.6 Hz, 2H), 5.65 (s, 2H), 4.11 (d, J = 5.6 Hz, 2H), 3.51 (s, 3H) ppm 149 4-amino-N-(2-((4-(4′-cyano-[1,1′- 454 1H NMR (400 MHz, DMSO-d6) δ = 12.37 biphenyl]-3-yl)thiazol-2-yl)amino)- (br s, 1H), 8.42-8.26 (m, 1H), 2-oxoethyl)benzamide 7.99 (s, 1H), 7.97-7.93 (m, 5H), 7.81 (s, 1H), 7.62 (d, J = 8.8 Hz, 1H), 7.60-7.58 (m, 3H), 6.57 (d, J = 8.8 Hz, 2H), 5.66 (s, 2H), 4.12 (d, J = 5.6 Hz, 2H). ppm 150 4-amino-N-(2-((4-(3-methyl-4-oxo-3,4- 435.1 1H NMR (400 MHz, DMSO) δ = 12.53 (br s, dihydrophthalazin-6-yl)thiazol- 1H), 8.82 (s, 1H), 2-yl)amino)-2-oxoethyl)benzamide 8.46-8.42 (m, 3H), 8.01-7.99 (m, 2H), 7.62 (d, J = 8.6 Hz, 2H), 6.57 (d, J = 8.6 Hz, 2H), 5.68 (s, 2H), 4.13 (d, J = 5.6 Hz, 2H), 3.74 (s, 3H) ppm 151 4-amino-N-(2-oxo-2-((4-(3-(1-(2,2,2- 500.8 1H NMR (400 MHz, DMSO-d6) δ = 12.31 trifluoroethyl)-1H-pyrazol-3- (br s, 1H), 8.57-8.32 (m, 2H), yl)phenyl)thiazol-2-yl)amino)ethyl)benzamide 7.93 (d, J = 2.3 Hz, 1H), 7.85 (d, J = 8.0 Hz, 1H), 7.76 (d, J = 7.6 Hz, 1H), 7.69 (s, 1H), 7.61 (d, J = 8.4 Hz, 2H), 7.49- 7.48 (m, 1H), 6.89 (d, J = 2.4 Hz, 1H), 6.56 (d, J = 8.4 Hz, 2H), 5.66 (s, 2H), 5.21-5.20 (m, 2H), 4.12 (d, J = 5.6 Hz, 2H). ppm 152 4-amino-N-(2-((4-(3′-fluoro-[1,1′- 447.1 1H NMR (400 MHz, DMSO-d6) δ = 12.34 biphenyl]-3-yl)thiazol-2-yl)amino)- (br s, 1H), 8.42-8.41 (m, 1H), 2-oxoethyl)benzamide 8.22 (d, J = 1.6 Hz, 1H), 7.93-7.92 (m, 1H), 7.80 (s, 1H), 7.63-7.62 (m, 1H), 7.60-7.54 (m, 6H), 7.24-7.23 (m, 1H), 6.57 (d, J = 8.8 Hz, 2H), 5.65 (s, 2H), 4.12 (d, J = 6.0 Hz, 2H) ppm 153 4-amino-N-(2-((4-(4′-(hydroxymethyl)- 458.8 1H NMR (400 MHz, DMSO-d6) δ = 8.49- [1,1′-biphenyl]-3- 8.38 (m, 2H), 8.20 (s, 1H), yl)thiazol-2-yl)amino)-2- 7.89 (d, J = 7.8 Hz, 1H), 7.76 (s, 1H), 7.69 oxoethyl)benzamide (d, J = 8.2 Hz, 2H), 7.62 (d, J = 8.4 Hz, 3H), 7.56-7.49 (m, 1H), 7.45 (d, J = 8.0 Hz, 2H), 6.57 (d, J = 8.6 Hz, 2H), 5.67 (s, 2H), 5.25 (br s, 1H), 4.57 (s, 2H), 4.13 (d, J = 5.6 Hz, 2H) ppm 154 4-amino-N-(2-((4-(3′-(hydroxymethyl)- 459.1 1H NMR (400 MHz, DMSO-d6) δ = 12.37 [1,1′-biphenyl]-3- (br s, 1H), 8.42 (s, 1H), yl)thiazol-2-yl)amino)-2- 8.20 (s, 1H), 7.94-7.85 (m, 1H), 7.75 (s, oxoethyl)benzamide 1H), 7.69-7.56 (m, 5H), 7.55-7.49 (m, 1H), 7.45 (m, 1H), 7.34 (br d, J = 7.6 Hz, 1H), 6.56 (d, J = 8.4 Hz, 2H), 5.66 (s, 2H), 5.28- 5.27 (m, 1H), 4.59 (d, J = 6.0 Hz, 2H), 4.12 (br d, J = 5.6 Hz, 2H) ppm 155 (R)-4-amino-N-(4-(methylthio)-1-oxo-1- 504.3 1H NMR (400 MHz, DMSO-d6) δ = 12.38 ((4-(3-(pyridin-4-yl)phenyl)thiazol- (s, 1H), 8.67 (d, J = 6.0 Hz, 2H), 2-yl)amino)butan-2-yl)benzamide 8.30 (s, 1H), 8.20 (d, J = 6.8 Hz, 1H), 8.01 (d, J = 7.6 Hz, 1H), 7.82 (s, 1H), 7.76-7.75 (m, 3H), 7.65 (d, J = 8.4 Hz, 2H), 7.61-7.57 (m, 1H), 6.56 (d, J = 8.8 Hz, 2H), 5.65-5.64 (m, 2H), 4.72-4.67 (m, 1H), 2.64-2.60 (m, 2H), 2.12-2.06 (m, 5H) ppm 156 4-(3-(2-(2-(4- 446.2 1H NMR (400 MHz, DMSO) δ = 12.36 (s, aminobenzamido)acetamido)thiazol- 1H), 8.42 (s, 1H), 8.31 (d, J = 7.6 Hz, 2H), 4-yl)phenyl)pyridine 1-oxide 8.27 (s, 1H), 7.97 (s, 1H), 7.83-7.81 (m, 3H), 7.73 (d, J = 8.8 Hz, 1H), 7.61 (d, J = 8.4 Hz, 2H), 7.58-7.56 (m, 1H), 6.57 (d, J = 8.4 Hz, 2H), 4.12 (d, J = 5.6 Hz, 2H) ppm 157 4-amino-N-(2-((4-(3′-cyano-[1,1′- 454.2 1H NMR (400 MHz, DMSO) δ = 12.36 (br s, biphenyl]-3-yl)thiazol-2-yl)amino)- 1H), 8.42 (br s, 1H), 2-oxoethyl)benzamide 8.25 (br d, J = 10.0 Hz, 2H), 8.15-7.94 (m, 2H), 7.89-7.55 (m, 7H), 6.57 (d, J = 3.8 Hz, 2H), 5.66 (s, 2H), 4.12 (s, 2H) ppm 158 4-amino-N-(2-((4-(4′-(aminomethyl)- 462.3 1H NMR (400 MHz, DMSO) δ = 12.31- 2′,3′,4′,5′-tetrahydro-[1,1′- 12.24 (m, 1H), 8.45-8.43 (m, 1H), biphenyl]-3-yl)thiazol-2-yl)amino)-2- 7.95 (s, 1H), 7.78-7.77 (m, 3H), 7.67 (s, oxoethyl)benzamide 1H), 7.62 (d, J = 7.2 Hz, 2H), 7.39-7.38 (m, 2H), 6.58 (d, J = 8.8 Hz, 2H), 6.21 (br s, 1H), 4.12 (d, J = 5.6 Hz, 2H), 2.89-2.81 (m, 2H), 2.38 (br d, J = 17.2 Hz, 3H), 1.99-1.93 (m, 3H), 1.46-1.40 (m, 1H) ppm 159 4-amino-N-(2-oxo-2-((4-(3-(2- 514.3 1H NMR (400 MHz, DMSO) δ = 12.36 (s, (piperazin-1-yl)pyridin-4- 1H), 8.81 (br s, 2H), yl)phenyl)thiazol-2- 8.47-8.44 (m, 1H), 8.26-8.24 (m, 2H), 7.98 yl)amino)ethyl)benzamide (d, J = 8.0 Hz, 1H), 7.79 (s, 1H), 7.72 (d, J = 8.0 Hz, 1H), 7.63- 7.58 (m, 3H), 7.24 (s, 1H), 7.12 (d, J = 6.4 Hz, 1H), 6.60 (d, J = 8.4 Hz, 2H), 4.13 (d, J = 5.6 Hz, 2H), 3.85-3.82 (m, 4H), 3.23-3.017 (m, 4H) ppm 160 4-amino-N-(2-((4-(3-(2-((2- 488.2 1H NMR (400 MHz, MeOD) δ = 8.30 (s, aminoethyl)amino)pyridin-4- 1H), 8.14-8.04 (m, 2H), yl)phenyl)thiazol-2-yl)amino)-2- 7.77-7.71 (m, 3H), 7.65-7.57 (m, 2H), 7.35- oxoethyl)benzamide 7.25 (m, 2H), 6.82-6.74 (m, 2H), 4.29 (s, 2H), 3.81-3.80 (m, 2H), 3.30-3.28 (m, 2H) ppm 161 4-amino-N-(2-((4-(3- 378 1H NMR (400 MHz, DMSO-d6) δ = 12.38 cyanophenyl)thiazol-2-yl)amino)- (br s, 1H), 8.43-8.42 (m, 1H), 2-oxoethyl)benzamide 8.32 (s, 1H), 8.23-8.21 (m, 1H), 7.85 (s, 1H), 7.78 (d, J = 7.6 Hz, 1H), 7.69-7.64 (m, 1H), 7.64-7.57 (m, 2H), 6.56 (d, J = 8.8 Hz, 2H), 5.65 (s, 2H), 4.12 (d, J = 5.6 Hz, 2H) ppm 162 4-amino-N-(2-oxo-2-((4-(3-(1- 503.1 1H NMR (400 MHz, DMSO-d6) δ = 12.37 (tetrahydro-2H-pyran-4-yl)-1H- (br s, 1H), 8.43-8.42 (m, 1H), pyrazol-3-yl)phenyl)thiazol-2- 8.37 (s, 1H), 7.87 (d, J = 2.0 Hz, 1H), 7.81 yl)amino)ethyl)benzamide (br d, J = 7.6 Hz, 1H), 7.73 (br d, J = 7.6 Hz, 1H), 7.67 (s, 1H), 7.62 (br d, J = 8.4 Hz, 2H), 7.45-7.44 (m, 1H), 6.75 (d, J = 2.0 Hz, 1H), 6.57 (d, J = 8.4 Hz, 2H), 5.66 (s, 2H), 4.52-4.40 (m, 1H), 4.12 (br d, J = 5.6 Hz, 2H), 4.00-3.97 (m, 2H), 3.54-3.45 (m, 2H), 2.06-1.96 (m, 4H) ppm 163 4-amino-N-(2-((4-(3-(2-(2- 489 1H NMR (400 MHz, MeOD) δ = 8.28-8.21 aminoethoxy)pyridin-4- (m, 2H), 8.02 (d, J = 7.8 Hz, 1H), yl)phenyl)thiazol-2-yl)amino)-2- 7.77-7.70 (m, 2H), 7.67 (d, J = 7.8 Hz, 1H), oxoethyl)benzamide 7.60-7.51 (m, 2H), 7.39 (dd, J = 5.6Hz, 1.6 Hz, 1H), 7.22 (d, J = 1.2 Hz, 1H), 6.81-6.71 (m, 2H), 4.66-4.58 (m, 2H), 4.29 (s, 2H), 3.45-3.39 (m, 2H) ppm 164 4-amino-N-(2-((4-(3-(2- 459.2 1H NMR (400 MHz, DMSO) δ = 8.61 (d, (aminomethyl)pyridin-4- J = 4.8 Hz, 1H), 8.44-8.41 (m, 1H), yl)phenyl)thiazol-2-yl)amino)- 8.32-8.30 (m, 2H), 8.01 (d, J = 8.0 Hz, 1H), 2-oxoethyl)benzamide 7.84 (s, 1H), 7.79 (s, 1H), 7.77-7.74 (m, 1H), 7.66-7.58 (m, 4H), 6.58- 6.54 (m, 2H), 5.66 (s, 2H), 4.12 (d, J = 5.6 Hz, 2H), 4.01 (s, 2H) ppm 165 4-amino-N-(2-((4-(3-(2- 460.2 1H NMR (400 MHz, MeOD) δ = 8.74 (d, (hydroxymethyl)pyridin-4- J = 6.2 Hz, 1H), 8.49 (s, 1H), yl)phenyl)thiazol-2-yl)amino)-2- 8.34 (s, 1H), 8.28 (br d, J = 6.2 Hz, 1H), oxoethyl)benzamide 8.19 (d, J = 7.8 Hz, 1H), 7.91 (d, J = 8.6 Hz, 1H), 7.78-7.72 (m, 2H), 7.69-7.67 (m, 1H), 7.63 (s, 1H), 6.85-6.77 (m, 2H), 5.08 (s, 2H), 4.30 (s, 2H) ppm 166 4-amino-N-(2-((4-(3-(1-(2-aminoethyl)- 489.2 1H NMR (400 MHz, MeOD) δ = 8.25 (s, 2-oxo-1,2-dihydropyridin-4- 1H), 8.04 (d, J = 8.0 Hz, 1H), yl)phenyl)thiazol-2-yl)amino)-2- 7.81-7.69 (m, 3H), 7.66 (br d, J = 7.2 Hz, oxoethyl)benzamide 1H), 7.60-7.53 (m, 2H), 6.92 (d, J = 2.0 Hz, 1H), 6.86 (dd, J = 7.2 Hz, 2.0 Hz, 1H), 6.80 (d, J = 8.8 Hz, 2H), 4.38-4.34 (m, 2H), 4.29 (s, 2H), 3.40 (m, 2H) ppm 167 4-amino-N-(2-((4-(2-(2- 491 1H NMR (400 MHz, DMSO) δ = 12.42 (br s, (dimethylamino)ethyl)-1-oxo-1,2- 1H), 8.80 (d, J = 2.0 Hz, 1H), dihydroisoquinolin-7-yl)thiazol- 8.43-8.40 (m, 1H), 8.21-8.19 (m, 1H), 8.15 2-yl)amino)-2-oxoethyl)benzamide (s, 1H), 7.76 (s, 1H), 7.69 (d, J = 8.4 Hz, 1H), 7.62 (d, J = 8.8 Hz, 2H), 7.45 (d, J = 7.2 Hz, 1H), 6.62 (d, J = 7.2 Hz, 1H), 6.57 (d, J = 8.8 Hz, 2H), 5.65 (s, 2H), 4.13 (d, J = 6.0 Hz, 2H), 4.08-4.05 (m, 2H), 2.58-2.55 (m, 2H), 2.20 (s, 6H) ppm 168 4-amino-N-(2-((4-(3-(2- 487 1H NMR (400 MHz, DMSO) δ = 12.37 (s, ((dimethylamino)methyl)pyridin- 1H), 10.01 (s, 1H), 4-yl)phenyl)thiazol-2-yl)amino)-2- 8.78 (d, J = 5.2 Hz, 1H), 8.46-8.43 (m, 1H), oxoethyl)benzamide 8.32 (s, 1H), 8.04 (d, J = 8.0 Hz, 1H), 7.94 (s, 1H), 7.88- 7.86 (m, 1H), 7.80-7.75 (m, 2H), 7.65-7.61 (m, 3H), 6.58 (d, J = 8.4 Hz, 2H), 4.53 (s, 2H), 4.13 (d, J = 5.6 Hz, 2H), 2.87 (s, 6H) ppm 169 4-amino-N-(2-((4-(3-(4- 464.2 1H NMR (400 MHz, MeOD) δ = 7.78 (br d, (aminomethyl)cyclohexyl)phenyl)thiazol- J = 13.2 Hz, 1H), 7.71-7.67 (m, 3H), 7.36- 2-yl)amino)-2-oxoethyl)benzamide 7.35 (m, 2H), 7.32-7.16 (m, 1H), 6.70 (d, J = 8.6 Hz, 2H), 4.25 (s, 2H), 3.08 (d, J = 7.6 Hz, 1H), 2.86 (d, J = 7.2 Hz, 1H), 2.72-2.55 (m, 1H), 1.80-1.89 (m, 2H), 1.95-1.75 (m, 5H), 1.61- 1.58 (m, 1H), 1.29-1.23 (m, 1H) ppm 170 4-amino-N-(2-((4-(3-(1-(2- 517.3 1H NMR (400 MHz, MeOD) δ = 8.40 (br s, (dimethylamino)ethyl)-6-oxo-1,6- 1H), 8.10-8.05 (m, 2H), dihydropyridin-3-yl)phenyl)thiazol- 7.99 (d, J = 8.0 Hz, 1H), 7.90 (d, J = 8.0 2-yl)amino)-2-oxoethyl)benzamide Hz, 1H), 7.70 (d, J = 8.0 Hz, 2H), 7.51-7.47 (m, 3H), 8.73-8.70 (m, 3H), 4.39- 4.36 (m, 2H), 4.28 (s, 2H), 3.22-3.19 (m, 2H), 2.72 (s, 6H) ppm 171 4-amino-N-(2-((4-(2-(2-aminoethyl)-1- 463 1H NMR (400 MHz, DMSO) δ = 8.82 (d, oxo-1,2-dihydroisoquinolin-7-yl)thiazol- J = 1.6 Hz, 1H), 8.46-8.43 (m, 1H), 2-yl)amino)-2-oxoethyl)benzamide 8.31 (br s, 1H), 8.23-8.21 (m, 1H), 7.77 (s, 1H), 7.71 (d, J = 8.4 Hz, 1H), 7.62 (d, J = 8.8 Hz, 2H), 7.46 (d, J = 7.2 Hz, 1H), 6.65 (d, J = 7.2 Hz, 1H), 6.57 (d, J = 8.4 Hz, 2H), 5.66 (br s, 2H), 4.13 (d, J = 5.6 Hz, 2H), 4.06 (br s, 2H), 2.99 (br s, 2H) ppm 172 (S)-4-amino-N-(3-hydroxy-1-oxo-1-((4- 460.2 1H NMR (400 MHz, DMSO-d6) δ = 12.35 (s, (3-(pyridin-4-yl)phenyl)thiazol- 1H), 8.92 (d, J = 6.4 Hz, 2H), 2-yl)amino)propan-2-yl)benzamide 8.44 (s, 1H), 8.28 (d, J = 6.4 Hz, 2H), 8.12 (d, J = 8.0 Hz, 1H), 8.00 (d, J = 7.2 Hz, 1H), 7.92 (br d, J = 8.8 Hz, 1H), 7.87 (s, 1H), 7.69-7.64 (m, 3H), 6.61 (d, J = 8.8 Hz, 2H), 4.72-4.68 (m, 1H), 3.85-3.83 (m, 2H) ppm 173 4-amino-N-(2-oxo-2-((4-(1-(pyridin-3- 437.4 1H NMR (400 MHz, DMSO) δ = 12.17 (s, yl)piperidin-3-yl)thiazol-2- 1H), 8.39-8.37 (m, 1H), yl)amino)ethyl)benzamide 8.32 (d, J = 2.6 Hz, 1H), 7.96-7.95 (m, 1H), 7.60 (d, J = 8.6 Hz, 2H), 7.37-7.32 (m, 1H), 7.22-7.20 (m, 1H), 6.91 (s, 1H), 6.56 (d, J = 8.6 Hz, 2H), 5.65 (s, 2H), 4.07 (d, J = 5.8 Hz, 2H), 3.93 (d, J = 11.0 Hz, 1H), 3.78 (d, J = 11.4 Hz, 1H), 2.97-2.75 (m, 3H), 2.07 (d, J = 8.0 Hz, 1H), 1.84-1.78 (m, 1H), 1.68-1.66 (m, 2H) ppm 174 4-amino-N-(2-((4-(3-((1r,4r)-4- 464.3 1H NMR (400 MHz, MeOD) δ = 7.78-7.67 (aminomethyl)cyclohexyl)phenyl)thiazol- (m, 4H), 7.37-7.27 (m, 2H), 2-yl)amino)-2-oxoethyl)benzamide 7.17 (d, J = 7.6 Hz, 1H), 6.91-6.76 (m, 2H), 4.26 (s, 2H), 2.85 (d, J = 7.0 Hz, 2H), 2.62- 2.53 (m, 1H), 2.03-1.92 (m, 4H), 1.74-1.72 (m, 1H), 1.65-1.52 (m, 2H), 1.31-1.17 (m, 2H) ppm 175 4-amino-N-(2-((4-(3-((1s,4s)-4- 464.3 1H NMR (400 MHz, MeOD) δ = 7.85-7.75 (aminomethyl)cyclohexyl)phenyl)thiazol- (m, 3H), 7.71 (br d, J = 8.0 Hz, 1H), 7.41- 2-yl)amino)-2-oxoethyl)benzamide 7.30 (m, 2H), 7.24 (d, J = 7.8 Hz, 1H), 6.97 (br s, 2H), 4.29 (d, J = 2.6 Hz, 2H), 3.09 (d, J = 7.6 Hz, 2H), 2.73 (br s, 1H), 2.07 (br s, 1H), 1.80 (br s, 8H) ppm 176 2-(4-(3-(2-(2-(4- 477.2 1H NMR (400 MHz, DMSO-d6) δ = 13.13 (s, aminobenzamido)acetamido)thiazol-4- 1H), 12.35 (s, 1H), 8.43-8.40 (m, 1H), 8.18 yl)phenyl)-1H-pyrazol-1-yl)acetic acid (s, 1H), 8.09 (s, 1H), 7.94 (s, 1H), 7.74 (br d, J = 8.0 Hz, 1H), 7.71 (s, 1H), 7.61 (d, J = 8.8 Hz, 2H), 7.52 (br d, J = 8.0 Hz, 1H), 7.44-7.40 (m, 1H), 6.59-6.55 (m, 2H), 5.67 (br s, 2H), 4.99 (s, 2H), 4.12 (d, J = 6.0 Hz, 2H) ppm 177 methyl 2-(4-(3-(2-(2-(4- 502.3 1H NMR (400 MHz, DMSO) δ = 12.37 (s, aminobenzamido)acetamido)thiazol- 1H), 8.58 (d, J = 5.2 Hz, 1H), 4-yl)phenyl)pyridin-2-yl)acetate 8.43-8.40 (m, 1H), 8.28 (s, 1H), 8.01 (br d, J = 8.0 Hz, 1H), 7.80 (s, 1H), 7.74-7.72 (m, 2H), 7.66-7.57 (m, 4H), 6.56 (d, J = 8.8 Hz, 2H), 5.65 (s, 2H), 4.12 (br d, J = 5.6 Hz, 2H), 3.94 (s, 2H), 3.64 (s, 3H) ppm 178 (S)-4-amino-N-(4-methoxy-1-oxo-1-((4- 488.2 1H NMR (400 MHz, MeOD) δ = 8.88 (d, (3-(pyridin-4-yl)phenyl)thiazol-2- J = 7.2 Hz, 2H), 8.50 (s, 1H), yl)amino)butan-2- 8.43 (d, J = 7.2 Hz, 2H), 8.20 (d, J = 8.0 Hz, yl)benzamide 1H), 7.92 (d, J = 8.8 Hz, 1H), 7.72 (d, J = 8.4 Hz, 2H), 7.70-7.66 (m, 1H), 7.64 (s, 1H), 6.81-6.78 (m, 2H), 4.85-4.82 (m, 1H), 3.61-3.58 (m, 2H), 3.37 (s, 3H), 2.32-2.23 (m, 1H), 2.20-2.12 (m, 1H) ppm 179 4-amino-N-(2-((4-(3- 438.3 1H NMR (400 MHz, DMSO) δ = 12.29 (s, morpholinophenyl)thiazol-2- 1H), 8.41-8.38 (m, 1H), yl)amino)-2-oxoethyl)benzamide 7.63-7.57 (m, 3H), 7.46 (s, 1H), 7.38-7.32 (m, 1H), 7.31-7.24 (m, 1H), 6.92 (d, J = 8.0 Hz, 1H), 6.56 (d, J = 8.8 Hz, 2H), 5.65 (s, 2H), 4.10 (d, J = 5.6 Hz, 2H), 3.78-3.74 (m, 4H), 3.18-3.12 (m, 4H) ppm 180 methyl 2-(4-(3-(2-(2-(4- 491.2 1H NMR (400 MHz, DMSO) δ = 12.25 (br s, aminobenzamido)acetamido)thiazol-4- 1H), 8.43-8.40 (m, 1H), yl)phenyl)-1H-pyrazol-1-yl)acetate 8.20 (s, 1H), 8.09 (s, 1H), 7.97 (s, 1H), 7.74 (d, J = 7.6 Hz, 1H), 7.70 (s, 1H), 7.61 (d, J = 8.8 Hz, 2H), 7.53 (d, J = 8.0 Hz, 1H), 7.44-7.40 (m, 1H), 6.56 (d, J = 8.8 Hz, 2H), 5.66 (s, 2H), 5.13 (s, 2H), 4.12 (d, J = 5.6 Hz, 2H), 3.71 (s, 3H) ppm 181 methyl 4-(3-(2-(2-(4- 488.2 1H NMR (400 MHz, DMSO) δ = 12.36 (s, aminobenzamido)acetamido)thiazol-4- 1H), 8.81 (d, J = 5.2 Hz, 1H), yl)phenyl)picolinate 8.40-8.35 (m, 3H), 8.06-8.02 (m, 2H), 7.83- 7.80 (m, 2H), 7.64-7.60 (m, 3H), 6.56 (d, J = 8.4 Hz, 2H), 5.65 (s, 2H), 4.11 (br d, J = 6.0 Hz, 2H), 3.93 (s, 3H) ppm 182 methyl 2-(4-(3-(2-(2-(4- 530.1 1H NMR (400 MHz, DMSO) δ = 12.36 (s, aminobenzamido)acetamido)thiazol-4- 1H), 8.58 (d, J = 5.2 Hz, 1H), yl)phenyl)pyridin-2-yl)-2- 8.44-8.41 (m, 1H), 8.25 (s, 1H), 8.01 (d, methylpropanoate J = 8.0 Hz, 1H), 7.82 (s, 1H), 7.75 (d, J = 7.6 Hz, 1H), 7.68 (s, 1H), 7.62- 7.57 (m, 4H), 6.56 (d, J = 8.4 Hz, 2H), 5.66 (s, 2H), 4.12 (d, J = 6.0 Hz, 2H), 3.60 (s, 3H), 1.60 (s, 6H) ppm 183 4-amino-N-(2-((4-(3-(2-(N- 501.4 1H NMR (400 MHz, DMSO) δ = 12.38 (s, methylacetamido)pyridin-4- 1H), 8.57 (d, J = 5.2 Hz, 1H), yl)phenyl)thiazol-2-yl)amino)-2- 8.47-8.46 (m, 1H), 8.31 (s, 1H), 8.03 (d, oxoethyl)benzamide J = 7.8 Hz, 1H), 7.89 (s, 1H), 7.83 (s, 1H), 7.79 (d, J = 7.6 Hz, 1H), 7.69- 8.68 (m, 1H), 7.67-7.56 (m, 3H), 6.62 (d, J = 8.4 Hz, 2H), 4.18-4.10 (m, 2H), 3.34 (s, 3H), 2.08 (s, 3H) ppm 184 5-amino-N-(2-oxo-2-((4-(3-(pyridin-4- 431.1 1H NMR (400 MHz, DMSO) δ = 8.69-8.67 yl)phenyl)thiazol-2- (m, 2H), 8.65-8.62 (m, 1H), yl)amino)ethyl)picolinamide 8.30 (s, 1H), 8.02 (d, J = 8.0 Hz, 1H), 7.97 (d, J = 2.4 Hz, 1H), 7.83 (s, 1H), 7.77-7.61 (m, 3H), 7.72 (d, J = 8.4 Hz, 1H), 7.62-7.58 (m, 1H), 7.01-6.98 (m, 1H), 5.99 (s, 2H), 4.21 (d, J = 6.0 Hz, 2H) ppm 185 4-amino-N-(2-((4-(3-(1,1- 486.4 1H NMR (400 MHz, DMSO) δ = 12.31 (br s, dioxidothiomorpholino)phenyl)thiazol- 1H), 8.40 (s, 1H), 2-yl)amino)-2-oxoethyl)benzamide 7.67-7.58 (m, 3H), 7.51 (s, 1H), 7.41-7.36 (m, 1H), 7.33-7.27 (m, 1H), 7.02-6.95 (m, 1H), 6.56 (d, J = 8.8 Hz, 2H), 5.66 (s, 2H), 4.10 (d, J = 5.6 Hz, 2H), 3.81 (br s, 4H), 3.16 (br s, 4H) ppm 186 4-amino-N-[2-[[4-[3-[2-[2-[2- 558.3 1H NMR (400 MHz, DMSO) δ = 12.60 (s, (dimethylamino)ethylamino]-2-oxo- 1H), 8.56 (d, J = 5.2 Hz, 1H), 8.43-8.40 (m, ethyl]-4-pyridyl]phenyl]thiazol-2- 1H), 8.27 (s, 1H), 8.18 (s, 1H), 8.07-8.06 yl]amino]-2-oxo-ethyl]benzamide (m, 1H), 8.00 (br d, J = 7.6 Hz, 1H), 7.79 (s, 1H), 7.72-7.69 (m, 2H), 7.62-7.57 (m, 4H), 6.56 (d, J = 8.4 Hz, 2H), 5.65 (s, 2H), 4.12 (br d, J = 6.0 Hz, 2H), 3.69 (s, 2H), 3.20- 3.15 (m, 2H), 2.33-2.32 (m, 2H), 2.14 (s, 6H) ppm 187 1-[3-[2-[[2-[(4- 480.1 1H NMR (400 MHz, MeOD) δ = 7.71-7.66 aminobenzoyl)amino]acetyl]amino]thiazol- (m, 2H), 7.56 (s, 1H), 7.38-7.33 (m, 2H), 4-yl]phenyl]piperidine- 7.26-7.24 (m, 1H), 6.95-6.94 (m, 1H), 6.72- 4-carboxylic acid 6.67 (m, 2H), 4.26 (s, 2H), 3.70 (d, J = 12.8 Hz, 2H), 2.89-2.77 (m, 2H), 2.46-2.45 (m, 1H), 2.09-1.99 (m, 2H), 1.92-1.78 (m, 2H) ppm 188 4-amino-N-[2-[[4-[4-methyl-3-(4- 444.1 1H NMR (400 MHz, DMSO) δ = 12.30 (br s, pyridyl)phenyl]thiazol-2-yl]amino]-2- 1H), 8.66 (d, J = 5.6 Hz, 2H), 8.42-8.40 (m, oxo-ethyl]benzamide 1H), 7.87 (br d, J = 8.0 Hz, 1H), 7.80 (s, 1H), 7.66 (s, 1H), 7.61 (br d, J = 8.8 Hz, 2H), 7.45 (d, J = 6.0 Hz, 2H), 7.41 (d, J = 8.0 Hz, 1H), 6.56 (d, J = 8.8 Hz, 2H), 5.66 (s, 2H), 4.10 (br d, J = 5.6 Hz, 2H), 2.28 (s, 3H) ppm 189 4-amino-N-[2-[[4-[3-[2-[2- 516.4 1H NMR (400 MHz, DMSO) δ = 12.38 (br (dimethylamino)ethylamino]-4- s, 1H), 8.42-8.44 (m, 1H), 8.32 (s, 1H), pyridyl]phenyl]thiazol-2-yl]amino]-2- 8.19 (s, 1H), 8.06 (d, J = 5.8 Hz, 1H), 7.96 oxo-ethyl]benzamide (br d, J = 7.6 Hz, 1H), 7.76 (s, 1H), 7.65- 7.50 (m, 4H), 6.83-6.79 (m, 2H), 6.57 (d, J = 8.6 Hz, 2H), 6.42 (br s, 1H), 5.67 (br s, 2H), 4.13 (br d, J = 5.6 Hz, 2H), 3.41 (br s, 2H), 2.47 (br s, 2H), 2.22 (s, 6H) ppm 190 4-amino-N-[2-[[4-[3-[2-(4- 528.2 1H NMR (400 MHz, MeOD) δ = 8.27 (s, methylpiperazin-1-yl)-4- 1H), 8.22 (d, J = 5.6 Hz, 1H), 8.03 (d, J = 8.0 pyridyl]phenyl]thiazol-2-yl]amino]-2- Hz, 1H), 7.79-7.77 (m, 2H), 7.72-7.70 (m, oxo-ethyl]benzamide 1H), 7.58-7.54 (m, 2H), 7.41 (br d, J = 6.4 Hz, 1H), 7.30-7.27 (m, 1H), 6.91-6.88 (m, 2H), 4.28-3.99 (m, 6H), 3.47 (br s, 4H), 2.99 (s, 3H) ppm 191 4-amino-N-[2-[[4-[3-[2-[2- 530.0 1H NMR (400 MHz, DMSO) δ = 12.35 (br s, (dimethylamino)ethyl-methyl-amino]-4- 1H), 8.43-8.41 (m, 1H), 8.23-8.14 (m, 2H), pyridyl]phenyl]thiazol-2-yl]amino]-2- 7.96 (d, J = 7.6 Hz, 1H), 7.78 (s, 1H), 7.67 oxo-ethyl]benzamide (d, J = 8.0 Hz, 1H), 7.61 (d, J = 8.8 Hz, 2H), 7.58-7.51 (m, 1H), 6.94-6.71 (m, 2H), 6.56 (d, J = 8.4 Hz, 2H), 5.66 (br s, 2H), 4.12 (d, J = 6.0 Hz, 2H), 3.72-3.69 (m, 2H), 3.08 (s, 3H), 2.47-2.44 (m, 2H), 2.22 (s, 6H) ppm 192 4-amino-N-[2-[[4-(1H-indazol-5- 392.9 1H NMR (400 MHz, DMSO-d6) δ = 13.09 (s, yl)thiazol-2-yl]amino]-2-oxo- 1H), 12.29 (s, 1H), 8.42-8.39 (m, 1H), ethyljbenzamide 8.27 (s, 1H), 8.11 (s, 1H), 7.91-7.87 (m, 1H), 7.62-7.53 (m, 4H), 6.56 (d, J = 8.4 Hz, 2H), 5.65 (s, 2H), 4.12 (d, J = 5.6 Hz, 2H) ppm 193 5-amino-N-[2-oxo-2-[[4-[3-(4- 432.2 1H NMR (400 MHz, DMSO) δ = 12.44 (s, pyridyl)phenyl]thiazol-2- 1H), 8.86-8.76 (m, 3H), 8.39 (s, 1H), 8.19 yl]amino]ethyl]pyrimidine-2- (s, 2H), 8.08 (d, J = 6.0 Hz, 3H), 7.89-7.83 carboxamide (m, 2H), 7.66-7.64 (m, 1H), 4.21 (d, J = 6.0 Hz, 2H) ppm 194 4-amino-N-[2-[[4-[3-(3-methyl-4- 444.2 1H NMR (400 MHz, DMSO) δ = 12.33 (br s, pyridyl)phenyl]thiazol-2-yl]amino]-2- 1H), 8.54 (s, 1H), 8.49 (d, J = 4.8 Hz, 1H), oxo-ethyl]benzamide 8.43-8.40 (m, 1H), 7.98 (d, J = 8.4 Hz, 1H), 7.94 (d, J = 1.6 Hz, 1H), 7.75 (s, 1H), 7.62 (d, J = 8.8 Hz, 2H), 7.57-7.55 (m, 1H), 7.38 (d, J = 8.0 Hz, 1H), 7.30 (d, J = 4.8 Hz, 1H), 6.57 (d, J = 8.8 Hz, 2H), 5.66 (s, 2H), 4.12 (d, J = 6.0 Hz, 2H), 2.29 (s, 3H) ppm 195 4-amino-N-[2-[[4-[3-(2-methyl-4- 444.0 1H NMR (400 MHz, DMSO) δ = 12.36 (m, pyridyl)phenyl]thiazol-2-yl]amino]-2- 1H), 8.53 (d, J = 5.2 Hz, 1H), 8.43-8.40 (m, oxo-ethyl]benzamide 1H), 8.28 (s, 1H), 7.99 (d, J = 7.6 Hz, 1H), 7.81 (s, 1H), 7.73 (br d, J = 7.6 Hz, 1H), 7.62 (d, J = 2.4 Hz, 2H), 7.60-7.53 (m, 3H), 6.56 (d, J = 8.8 Hz, 2H), 5.66 (s, 2H), 4.12 (d, J = 5.6 Hz, 2H), 2.55 (s, 3H) ppm 196 4-amino-N-[2-[[4-[3-[2- 473.0 1H NMR (400 MHz, METHANOL-d4) δ = (methylaminomethyl)-4- 8.72 (d, J = 5.2 Hz, 1H), 8.31 (s, 1H), 8.04 pyridyl]phenyl]thiazol-2-yl]amino]-2- (d, J = 7.6 Hz, 1H), 7.81-7.77 (m, 4H), oxo-ethyl]benzamide 7.73 (d, J = 8.0 Hz, 1H), 7.61-7.56 (m, 2H), 6.97-6.92 (m, 2H), 4.45 (s, 2H), 4.30 (s, 2H), 2.85 (s, 3H) ppm 197 4-amino-N-[2-oxo-2-[[4-[3-(4- 460.2 1H NMR (400 MHz, DMSO) δ = 12.33 (s, pyridyloxymethyl)phenyl]thiazol-2- 1H), 8.41-8.39 (m, 3H), 8.13 (s, 1H), 8.00 yl]amino]ethyl]benzamide (s, 1H), 7.88-7.84 (m, 1H), 7.64-7.59 (m, 3H), 7.48-7.40 (m, 2H), 7.06-7.05 (m, 2H), 6.56 (d, J = 8.4 Hz, 2H), 5.65 (br s, 2H), 5.24 (s, 1H), 5.25 (s, 1H), 4.11 (d, J = 5.6 Hz, 2H) ppm 198 4-amino-N-[2-[[4-[3-(1-hydroxy-1- 411.2 1H NMR (400 MHz, DMSO) δ = 12.35 (s, methyl-ethyl)phenyl]thiazol-2-yl]amino]- 1H), 8.46-8.43 (m, 1H), 8.04 (s, 1H), 7.71 2-oxo-ethyl]benzamide (br d, J = 7.6 Hz, 1H), 7.63 (d, J = 8.4 Hz, 2H), 7.57 (s, 1H), 7.42-7.40 (m, 1H), 7.36- 7.32 (m, 1H), 6.62 (d, J = 8.8 Hz, 2H), 4.12 (br d, J = 5.6 Hz, 2H), 1.45 (s, 6H) ppm 199 5-amino-N-[2-[[4-[3-(2-methoxy-4- 461.2 1H NMR (400 MHz, DMSO) δ = 12.43 (br s, pyridyl)phenyl]thiazol-2-yl]amino]-2- 1H), 8.64-8.62 (m, 1H), 8.27-8.23 (m, 2H), oxo-ethyl]pyridine-2-carboxamide 8.04-7.92 (m, 2H), 7.83 (s, 1H), 7.75-7.67 (m, 2H), 7.62-7.52 (m, 1H), 7.36-7.35 (m, 1H), 7.17 (s, 1H), 7.0-6.97 (m, 1H), 5.99 (s, 2H), 4.20 (d, J = 6.0 Hz, 2H), 3.92 (s, 3H) ppm 200 2-[[4-[3-[2-[[2-[(4- 517.3 1H NMR (400 MHz, DMSO) δ = 8.44-8.40 aminobenzoyl)amino]acetyl]amino]thiazol- (m, 1H), 8.29 (s, 1H), 8.20 (s, 1H), 8.13 (d, 4-yl]phenyl]-2-pyridyl]-methyl- J = 5.2 Hz, 1H), 7.97 (d, J = 7.6 Hz, 1H), 7.78 amino]acetic acid (s, 1H), 7.68 (d, J = 7.6 Hz, 1H), 7.61 (d, J = 8.8 Hz, 2H), 7.58-7.52 (m, 1H), 6.90 (d, J = 5.2 Hz, 1H), 6.85 (s, 1H), 6.56 (d, J = 8.8 Hz, 2H), 5.65 (br s, 2H), 4.25 (s, 2H), 4.12 (br d, J = 6.0 Hz, 2H), 3.12 (s, 3H) ppm 201 4-amino-N-[2-oxo-2-[[4-[6-(4-pyridyl)-2- 431.1 1H NMR (400 MHz, DMSO) δ = 12.44 (s, pyridyl]thiazol-2- 1H), 8.87 (d, J = 4.8 Hz, 2H), 8.49-8.42 (m, yl]amino]ethyl]benzamide 3H), 8.21-8.17 (m, 1H), 8.15-8.09 (m, 2H), 8.08-8.04 (m, 1H), 7.63 (d, J = 8.8 Hz, 2H), 6.59 (d, J = 8.8 Hz, 2H), 4.14 (d, J = 5.6 Hz, 2H) ppm 19F NMR (376MHz, DMSO-d6) ä = −74.27 (s, 1F) 202 4-amino-N-[2-[[4-[3-[2-[methyl-[2- 530.2 1H NMR (400 MHz, DMSO) δ = 12.36 (br s, (methylamino)-2-oxo-ethyl]amino]-4- 1H), 8.44-8.41 (m, 1H), 8.21 (s, 1H), 8.15 pyridyl]phenyl]thiazol-2-yl]amino]-2- (d, J = 5.2 Hz, 1H), 7.98 (d, J = 8.0 Hz, 1H), oxo-ethyl]benzamide 7.81-7.74 (m, 2H), 7.68 (d, J = 7.6 Hz, 1H), 7.62 (d, J = 8.8 Hz, 2H), 7.58-7.54 (m, 1H), 6.97-6.91 (m, 1H), 6.87 (s, 1H), 6.57 (d, J = 8.8 Hz, 2H), 5.66 (s, 2H), 4.20 (s, 2H), 4.13 (d, J = 6.0 Hz, 2H), 3.13 (s, 3H), 2.60 (d, J = 4.4 Hz, 3H) ppm 203 4-amino-N-[2-[[4-[3-[2-(1,1- 563.2 1H NMR (400 MHz, DMSO) δ = 12.38 (s, dioxothiazinan-2-yl)-4- 1H), 8.53 (d, J = 5.2 Hz, 1H), 8.42-8.28 (m, pyridyl]phenyl]thiazol-2-yl]amino]-2- 1H), 8.27 (s, 1H), 8.02 (d, J = 7.6 Hz, 1H), oxo-ethyl]benzamide 7.81 (s, 1H), 7.72 (br d, J = 8.0 Hz, 1H), 7.64-7.60 (m, 5H), 6.57 (d, J = 8.4 Hz, 2H), 5.65 (s, 2H), 4.13 (d, J = 5.6 Hz, 2H), 4.01- 3.98 (m, 2H), 3.33 (br s, 2H), 2.20-2.17 (m, 2H), 1.98-1.84 (m, 2H) ppm 204 4-amino-N-[2-[[4-[3-(4-methyl-3-oxo- 465.2 1H NMR (400 MHz, DMSO) δ = 12.33 (br s, piperazin-1-yl)phenyl]thiazol-2- 1H), 8.41 (m, 1H), 7.68-7.59 (m, 3H), 7.47 yl]amino]-2-oxo-ethyl]benzamide (s, 1H), 7.40-7.33 (m, 1H), 7.32-7.26 (m, 1H), 6.93 (m, 1H), 6.58 (d, J = 8.6 Hz, 2H), 5.67 (s, 2H), 4.11 (br d, J = 5.6 Hz, 2H), 3.82 (s, 2H), 3.56-3.52 (m, 2H), 3.47-3.44 (m, 2H), 2.92 (s, 3H) ppm 205 4-amino-N-[2-[[4-[3-[2-[2- 501.2 1H NMR (400 MHz, DMSO) δ = 12.40 (br s, (methylamino)-2-oxo-ethyl]-4- 1H), 8.57 (d, J = 5.4 Hz, 1H), 8.41 (br s, pyridyl]phenyl]thiazol-2-yl]amino]-2- 1H), 8.28 (s, 1H), 8.01 (br d, J = 7.8 Hz, oxo-ethyl]benzamide 2H), 7.79 (s, 1H), 7.75-7.68 (m, 2H), 7.67- 7.56 (m, 4H), 6.57 (br d, J = 8.6 Hz, 2H), 5.66 (s, 2H), 4.13 (br d, J = 5.6 Hz, 2H), 3.69 (s, 2H), 2.62 (d, J = 4.6 Hz, 3H) ppm 206 4-amino-N-[2-[[4-[3-[2-[[2- 544.2 1H NMR (400 MHz, DMSO) δ = 12.36 (s, (dimethylamino)-2-oxo-ethyl]-methyl- 1H), 8.44-8.41 (m, 1H), 8.21 (s, 1H), 8.12 amino]-4-pyridyl]phenyl]thiazol-2- (d, J = 5.2 Hz, 1H), 7.98 (d, J = 7.6 Hz, 1H), yl]amino]-2-oxo-ethyl]benzamide 7.79 (s, 1H), 7.68 (d, J = 8.4 Hz, 1H), 7.62 (d, J = 8.4 Hz, 2H), 7.59-7.53 (m, 1H), 6.92- 6.83 (m, 2H), 6.57 (d, J = 8.4 Hz, 2H), 5.66 (s, 2H), 4.52 (s, 2H), 4.13 (d, J = 5.6 Hz, 2H), 3.12-3.01 (m, 6H), 2.82 (s, 3H) ppm 207 4-amino-N-[2-[[4-[3-(2-isopropyl-4- 472.2 1H NMR (400 MHz, DMSO) δ = 12.37 (s, pyridyl)phenyl]thiazol-2-yl]amino]-2- 1H), 8.57 (d, J = 4.8 Hz, 1H), 8.45-8.42 (m, oxo-ethyl]benzamide 1H), 8.27 (s, 1H), 7.99 (d, J = 7.6 Hz, 1H), 7.82 (s, 1H), 7.74 (d, J = 8.4 Hz, 1H), 7.62- 7.54 (m, 5H), 6.56 (d, J = 8.8 Hz, 2H), 5.67 (s, 2H), 4.12 (d, J = 5.6 Hz, 2H), 3.15-3.08 (m, 1H), 1.29 (d, J = 6.8 Hz, 6H) ppm 208 4-amino-N-[2-oxo-2-[[4-[5-(4-pyridyl)-3- 431.2 1H NMR (400 MHz, DMSO) δ = 12.68- pyridyl]thiazol-2- 12.28 (m, 1H), 9.20 (d, J = 2.0 Hz, 1H), yl]amino]ethyl]benzamide 8.96 (d, J = 2.2 Hz, 1H), 8.74-8.70 (m, 2H), 8.62-8.61 (m, 1H), 8.44-8.42 (m, 1H), 7.99 (s, 1H), 7.87-7.84 (m, 2H), 7.62 (d, J = 8.6 Hz, 2H), 6.57 (d, J = 8.6 Hz, 2H), 5.66 (s, 2H), 4.13 (d, J = 5.6 Hz, 2H) ppm 209 methyl 2-[3-[3-[2-[[2-[(4- 519.2 1H NMR (400 MHz, DMSO) δ = 12.41 (s, aminobenzoyl)amino]acetyl]amino]thiazol- 1H), 8.44 (s, 1H), 8.34 (s, 1H), 8.00 (s, 4-yl]phenyl]pyrazol-1-yl]-2-methyl- 1H), 7.83 (d, J = 6.4 Hz, 1H), 7.77-7.68 (m, propanoate 2H), 7.63 (d, J = 7.2 Hz, 2H), 7.46 (s, 1H), 6.81 (s, 1H), 6.58 (d, J = 7.2 Hz, 2H), 5.68 (s, 2H), 4.13 (s, 2H), 3.65 (s, 3H), 1.84 (s, 6H) ppm 210 2-[3-[3-[2-[[2-[(4- 477.1 1H NMR (400 MHz, DMSO) δ = 12.45 (s, aminobenzoyl)amino]acetyl]amino]thiazol- 1H), 8.64-8.30 (m, 2H), 7.87-7.79 (m, 2H), 4-yl]phenyl]pyrazol-1-yl]acetic acid 7.76-7.68 (m, 2H), 7.63 (d, J = 8.8 Hz, 2H), 7.46 (m, 1H), 6.78 (d, J = 2.2 Hz, 1H), 6.58 (d, J = 8.6 Hz, 2H), 5.01 (s, 2H), 4.13 (br d, J = 5.6 Hz, 2H) ppm 211 4-amino-N-[2-[[4-[3-[2-(1-amino-1- 487.4 1H NMR (400 MHz, DMSO) δ = 8.61 (d, methyl-ethyl)-4-pyridyl]phenyl]thiazol- J = 5.2 Hz, 1H), 8.45-8.37 (m, 1H), 8.28 (s, 2-yl]amino]-2-oxo-ethyl]benzamide 1H), 8.04-7.94 (m, 2H), 7.83-7.72 (m, 2H), 7.64-7.53 (m, 5H), 6.56 (d, J = 8.8 Hz, 2H), 5.66 (s, 2H), 4.12 (d, J = 5.6 Hz, 2H), 1.49 (s, 6H) ppm 212 2-[3-[3-[2-[[2-[(4- 505.2 1H NMR (400 MHz, DMSO) δ = 12.42 (s, aminobenzoyl)amino]acetyl]amino]thiazol- 1H), 8.42 (s, 1H), 8.36 (s, 1H), 7.96 (d, 4-yl]phenyl]pyrazol-1-yl]-2-methyl- J = 2.4 Hz, 1H), 7.82 (br d, J = 8.0 Hz, 1H), propanoic acid 7.74 (br d, J = 7.6 Hz, 1H), 7.69 (s, 1H), 7.63 (d, J = 8.6 Hz, 2H), 7.46 (m, 1H), 6.79 (d, J = 2.4 Hz, 1H), 6.58 (d, J = 8.6 Hz, 2H), 4.14 (d, J = 5.6 Hz, 2H), 1.81 (s, 6H) ppm 213 4-amino-N-[2-[[4-[3-[1-[1,1-dimethyl-2- 518.3 1H NMR (400 MHz, DMSO) δ = 12.43 (br s, (methylamino)-2-oxo-ethyl]pyrazol-3- 1H), 8.43 (m, 1H), 8.37 (s, 1H), 7.92 (d, yl]phenyl]thiazol-2-yl]amino]-2-oxo- J = 2.4 Hz, 1H), 7.83 (d, J = 8.0 Hz, 1H), 7.76 ethyl]benzamide (d, J = 7.8 Hz, 1H), 7.69 (s, 1H), 7.62 (d, J = 8.8 Hz, 2H), 7.46 (m, 1H), 7.34 (br d, J = 4.4 Hz, 1H), 6.81 (d, J = 2.4 Hz, 1H), 6.57 (d, J = 8.8 Hz, 2H), 5.68 (s, 2H), 4.13 (d, J = 5.6 Hz, 2H), 2.59 (d, J = 4.4 Hz, 3H), 1.76 (s, 6H) ppm 214 (S)-4-amino-N-(1-((4-(3-(2- 518.1 1H NMR (400 MHz, DMSO) δ = 12.44 (s, methylpyridin-4-yl)phenyl)thiazol-2- 1H), 8.92-8.79 (m, 1H), 8.46 (s, 1H), 8.36- yl)amino)-4-(methylthio)-1-oxobutan-2- 8.09 (m, 4H), 7.94 (br d, J = 7.4 Hz, 1H), yl)benzamide 7.89 (s, 1H), 7.74-7.59 (m, 3H), 6.59 (br dd, J = 8.6, 2.6 Hz, 2H), 4.75-4.65 (m, 1H), 2.76 (d, J = 2.4 Hz, 3H), 2.66-2.52 (m, 3H), 2.15-2.04 (m, 5H) ppm 215 N-[1-[4-[3-[2-[[2-[(4- 597.5 1H NMR (400 MHz, DMSO) δ = 12.46- aminobenzoyl)amino]acetyl]amino]thiazol- 12.29 (m, 1H), 9.59 (s, 1H), 8.65 (d, J = 4-yl]phenyl]-2-pyridyl]-1-methyl- 5.2 Hz, 1H), 8.44-8.43 (m, 1H), 8.27 (s, ethyl]-5-methyl-1,3,4-oxadiazole-2- 1H), 8.01 (d, J = 7.6 Hz, 1H), 7.89 (s, 1H), carboxamide 7.81 (s, 1H), 7.75 (d, J = 7.6 Hz, 1H), 7.68- 7.67 (m, 1H), 7.58 (s, 3H), 6.57 (d, J = 8.8 Hz, 2H), 5.67 (s, 2H), 4.12 (d, J = 5.6 Hz, 2H), 2.58 (s, 3H), 1.81 (s, 6H) ppm 216 4-amino-N-[2-[[4-[3-[1-[2- 490.2 1H NMR (400 MHz, DMSO) δ = 12.45 (br s, (methylamino)-2-oxo-ethyl]pyrazol-3- 1H), 8.52-8.34 (m, 2H), 8.04 (br d, J = 4.8 yl]phenyl]thiazol-2-yl]amino]-2-oxo- Hz, 1H), 7.82 (br d, J = 7.8 Hz, 1H), 7.79 (d, ethyl]benzamide J = 2.4 Hz, 1H), 7.72 (br d, J = 7.8 Hz, 1H), 7.69 (s, 1H), 7.62 (d, J = 8.6 Hz, 2H), 7.46 (m, 1H), 6.77 (d, J = 2.4 Hz, 1H), 6.57 (d, J = 8.6 Hz, 2H), 5.68 (s, 2H), 4.84 (s, 2H), 4.13 (br d, J = 5.6 Hz, 2H), 2.65 (d, J = 4.6 Hz, 3H) ppm 217 4-amino-N-[2-oxo-2-[[4-[2-(4-pyridyl)-4- 431.2 1HNMR (400 MHz, DMSO) δ = 12.48 (m, pyridyl]thiazol-2- 1H), 8.78 (d, J = 4.8 Hz, 1H), 8.74-8.72 (m, yl]amino]ethyl]benzamide 2H), 8.52 (s, 1H), 8.45-8.42 (m, 1H), 8.18 (s, 1H), 8.10-8.09 (m, 2H), 7.61 (d, J = 8.8 Hz, 2H), 7.69-7.53 (m, 1H), 6.56 (d, J = 8.8 Hz, 2H), 5.66 (s, 2H), 4.13 (d, J = 5.6 Hz, 2H) ppm 218 4-amino-N-[2-oxo-2-[[4-[3-[2-(4- 458.2 1HNMR (400 MHz, MeOD) δ = 8.68 (d, pyridyl)ethyl]phenyl]thiazol-2- J = 6.8 Hz, 2H), 7.91 (d, J = 6.8 Hz, 2H), yl]amino]ethyl]benzamide 7.77-7.73 (m, 4H), 7.36 (s, 1H), 7.31-7.29 (m, 1H), 7.15 (d, J = 8.0 Hz, 1H), 6.86-6.84 (m, 2H), 4.27 (s, 2H), 3.36-3.34 (m, 2H), 3.16-3.12 (m, 2H) ppm 219 1-[4-[3-[2-[[2-[(4- 514.3 1H NMR (400 MHz, DMSO) δ = 13.40- aminobenzoyl)amino]acetyl]amino]thiazol- 12.88 (m, 1H), 12.53-12.29 (m, 1H), 8.60- 4-yl]phenyl]-2- 8.52 (m, 1H), 8.48-8.38 (m, 1H), 8.31-8.25 pyridyl]cyclopropanecarboxylic acid (m, 1H), 8.05-7.97 (m, 1H), 7.89-7.80 (m, 2H), 7.78-7.71 (m, 1H), 7.66-7.55 (m, 4H), 6.63-6.53 (m, 2H), 5.72-5.61 (m, 2H), 4.17- 4.09 (m, 2H), 1.61-1.44 (m, 4H) ppm 220 4-amino-N-[2-[[4-[3-(1-methylimidazol-4- 433.1 1H NMR (400 MHz, DMSO) δ = 8.43-8.40 yl)phenyl]thiazol-2-yl]amino]-2-oxo- (m, 1H), 8.35 (s, 1H), 8.19 (s, 1H), 7.72- ethyl]benzamide 7.60 (m, 7H), 7.40-7.38 (m, 1H), 6.56 (d, J = 8.8 Hz, 2H), 5.66 (s, 2H), 4.12 (d, J = 6.0 Hz, 2H), 3.70 (s, 3H) ppm 221 4-amino-N-[2-[[4-[3-[2-(3-amino-1- 528.3 1HNMR (400 MHz, DMSO) δ = 8.45-8.42 piperidyl)-4-pyridyl]phenyl]thiazol-2- (m, 1H), 8.35-8.32 (m, 1H), 8.20 (s, 1H), yl]amino]-2-oxo-ethyl]benzamide 8.18 (d, J = 5.2 Hz, 1H), 7.97 (d, J = 7.6 Hz, 1H), 7.79 (s, 1H), 7.69 (br d, J = 8.0 Hz, 1H), 7.61 (d, J = 8.8 Hz, 2H), 7.57-7.53 (m, 1H), 7.08 (s, 1H), 6.95 (d, J = 5.2 Hz, 1H), 6.56 (d, J = 8.4 Hz, 2H), 5.67 (s, 2H), 4.36-4.33 (m, 1H), 4.15-4.11 (m, 3H), 3.03- 2.78 (m, 4H), 2.00-1.88 (m, 1H), 1.77-1.67 (m, 2H), 1.57-1.34 (m, 2H) ppm 222 4-amino-N-[2-[[4-[3-(1,2- 447.2 1H NMR (400 MHz, DMSO) δ = 12.41 (s, dimethylimidazol-4-yl)phenyl]thiazol-2- 1H), 8.44-8.41 (m, 1H), 8.31-8.30 (m, 1H), yl]amino]-2-oxo-ethyl]benzamide 7.68-7.66 (m, 1H), 7.62-7.59 (m, 4H), 7.51 (s, 1H), 7.37-7.33 (m, 1H), 6.57-6.55 (m, 2H), 5.67 (s, 2H), 4.12 (d, J =6.0 Hz, 2H), 3.58 (s, 3H), 2.32 (s, 3H) ppm 223 4-amino-N-[2-[[4-[3-[2-[1- 527.3 1H NMR (400 MHz, DMSO) δ = 12.49- (methylcarbamoyl)cyclopropyl]-4- 12.20 (m, 1H), 8.61 (d, J = 5.4 Hz, 1H), pyridyl]phenyl]thiazol-2-yl]amino]-2- 8.46-8.38 (m, 1H), 8.27 (s, 1H), 8.01 (d, J = oxo-ethyl]benzamide 7.8 Hz, 1H), 7.82 (s, 1H), 7.74 (d, J = 7.8 Hz, 1H), 7.67-7.56 (m, 6H), 6.57 (d, J = 8.6 Hz, 2H), 5.67 (s, 2H), 4.13 (d, J = 5.8 Hz, 2H), 2.62 (d, J = 4.6 Hz, 3H), 1.43-1.37 (m, 2H), 1.30-1.24 (m, 2H) ppm 224 [2-[4-[3-[2-[[2-[(4- 488.4 1H NMR (400 MHz, METHANOL-d4) δ = aminobenzoyl)amino]acetyl]amino]thiazol- 8.46 (d, J = 5.2 Hz, 1H), 8.30 (s, 1H), 7.98 4-yl]phenyl]-2- (d, J = 8.0 Hz, 1H), 7.77 (s, 1H), 7.72 (d, pyridyl]acetyl]oxysodium J = 8.8 Hz, 2H), 7.67 (d, J = 7.6 Hz, 1H), 7.60 (d, J = 5.2 Hz, 1H), 7.53-7.49 (m, 1H), 7.36 (s, 1H), 6.70 (d, J = 8.4 Hz, 2H), 4.20 (s, 2H), 3.80 (s, 2H) 225 4-amino-N-[2-[[4-[3-[2-[3- 556.2 1H NMR (400 MHz, MeOD) δ = 8.29-8.27 (dimethylamino)-1-piperidyl]-4- (m, 1H), 8.17 (d, J = 6.0 Hz, 1H), 8.07-8.05 pyridyl]phenyl]thiazol-2-yl]amino]-2- (m, 1H), 7.78-7.73 (m, 3H), 7.59-7.56 (m, oxo-ethyl]benzamide 2H), 7.46 (s, 1H), 7.31-7.30 (m, 1H), 6.87- 6.83 (m, 2H), 4.50-4.47 (m, 1H), 4.27 (s, 2H), 4.09-4.05 (m, 1H), 3.68-3.63 (m, 1H), 3.54-3.47 (m, 1H), 3.39-3.34 (m, 1H), 3.00 (s, 6H), 2.30-2.26 (m, 1H), 2.08-1.77 (m, 3H) ppm 226 4-amino-N-[2-[[4-[3-[2-[1,1-dimethyl-2- 529.3 1H NMR (400 MHz, DMSO) δ = 12.48- (methylamino)-2-oxo-ethyl]-4- 12.23 (m, 1H), 8.61 (d, J = 5.4 Hz, 1H), pyridyl]phenyl]thiazol-2-yl]amino]-2- 8.47-8.37 (m, 1H), 8.24 (s, 1H), 8.01 (d, J = oxo-ethyl]benzamide 7.8 Hz, 1H), 7.82-7.68 (m, 2H), 7.66-7.55 (m, 5H), 7.40 (br d, J = 4.4 Hz, 1H), 6.57 (d, J = 8.8 Hz, 2H), 5.67 (s, 2H), 4.13 (br d, J = 5.4 Hz, 2H), 2.58 (d, J = 4.4 Hz, 3H), 1.56 (s, 6H) ppm 227 4-amino-N-[2-oxo-2-[[4-(3-pyrimidin-4- 431.2 1H NMR (400 MHz, DMSO) δ = 9.30 (s, ylphenyl)thiazol-2- 1H), 8.90 (d, J = 5.2 Hz, 1H), 8.82 (s, 1H), yl]amino]ethyl]benzamide 8.45-8.43 (m, 1H), 8.20-8.04 (m, 3H), 7.80 (s, 1H), 7.62 (d, J = 8.8 Hz, 3H), 6.57 (d, J = 8.4 Hz, 2H), 5.67 (s, 2H), 4.13 (d, J = 6.0 Hz, 2H) ppm 228 4-amino-N-[2-[[4-(1-methylindazol-6- 407.0 1H NMR (400 MHz, DMSO-d6) δ = 12.39 (s, yl)thiazol-2-yl]amino]-2-oxo- 1H), 8.45-8.43 (m, 1H), 8.11 (s, 1H), 8.04 ethyl]benzamide (d, J = 0.8 Hz, 1H), 7.82-7.76 (m, 1H), 7.75-7.67 (m, 2H), 7.62 (d, J = 8.8 Hz, 2H), 6.57 (d, J = 8.4 Hz, 2H), 5.68 (s, 2H), 4.13 (d, J = 5.6 Hz, 2H), 4.07 (s, 3H) ppm 229 4-amino-N-[2-[[4-[3-[1-(2-hydroxy-2- 491.4 1H NMR (400 MHz, DMSO-d6) δ = 12.37 methyl-propyl)pyrazol-3- (br s, 1H), 8.44-8.43 (m, 1H), 8.36 (s, 1H), yl]phenyl]thiazol-2-yl]amino]-2-oxo- 7.81 (d, J = 7.6 Hz, 1H), 7.75-7.72 (m, ethyl]benzamide 2H), 7.67 (s, 1H), 7.63 (d, J = 8.6 Hz, 2H), 7.45-7.44 (m, 1H), 6.75 (d, J = 2.0 Hz, 1H), 6.57 (d, J = 8.6 Hz, 2H), 5.66 (s, 2H), 4.92-4.56 (m, 1H), 4.13 (d, J = 5.6 Hz, 2H), 4.09 (s, 2H), 1.12 (s, 6H). 230 4-amino-N-[2-oxo-2-[[4-[3- 467.0 1H NMR (400 MHz, DMSO) δ (ppm) = (tetrahydropyran-4- 12.33 (br s, 1H), 8.43-8.40 (m, 1H), 7.88 yloxymethyl)phenyl]thiazol-2- (s, 1H), 7.81 (d, J = 7.6 Hz, 1H), 7.62-7.59 yl]amino]ethyl]benzamide (m, 3H), 7.42-7.38 (m, 1H), 7.30 (d, J = 7.6 Hz, 1H), 6.57 (d, J = 8.4 Hz, 2H), 5.66 (s, 2H), 4.58 (s, 2H), 4.12 (d, J = 5.6 Hz, 2H), 3.85-3.80 (m, 2H), 3.63-3.56 (m, 1H), 3.40- 3.35 (m, 2H), 1.99-1.85 (m, 2H), 1.55- 1.41 (m, 2H) 231 4-amino-N-[2-[[4-[3-(3-methylisoxazol- 434.2 1H NMR (400 MHz, DMSO-d6) δ = 12.36 5-yl)phenyl]thiazol-2-yl]amino]-2-oxo- (s, 1H), 8.43 (t, J = 6.0 Hz, 1H), 8.37 (s, ethyl]benzamide 1H), 8.01 (d, J = 8.0 Hz, 1H), 7.80-7.77 (m, 2H), 7.64-7.59 (m, 3H), 6.92 (s, 1H), 6.57 (d, J = 8.4 Hz, 2H), 5.66 (s, 2H), 4.12 (d, J = 6.0 Hz, 2H), 2.31 (s, 3H) 232 4-amino-N-[2-[[4-[3-[1-(2-methoxy-2- 505.1 1H NMR (400 MHz, DMSO) δ (ppm) = methyl-propyl)pyrazol-3- 12.48-12.30 (m, 1H), 8.43-8.40 (m, 1H), yl]phenyl]thiazol-2-yl]amino]-2-oxo- 8.34 (s, 1H), 7.83-7.78 (m, 1H), 7.73 (d, J = ethyl]benzamide 8.0 Hz, 1H), 7.69 (d, J = 2.4 Hz, 1H), 7.67 (s, 1H), 7.61 (d, J = 8.7 Hz, 2H), 7.44 (t, J = 7.8 Hz, 1H), 6.74 (d, J = 2.3 Hz, 1H), 6.56 (d, J = 8.4 Hz, 2H), 5.66 (s, 2H), 4.18 (s, 2H), 4.12 (d, J = 6.0 Hz, 2H), 3.20 (s, 3H), 1.12 (s, 6H) 233 (S)-4-amino-N-(4-(dimethylamino)-1- 501.3 1H NMR (400 MHz, DMSO) δ = 8.73-8.64 oxo-1-((4-(3-(pyridin-4- (m, 2H), 8.52 (d, J = 7.0 Hz, 1H), 8.31 (s, yl)phenyl)thiazol-2-yl)amino)butan-2- 1H), 8.02 (d, J = 7.6 Hz, 1H), 7.82 (s, 1H), yl)benzamide 7.79-7.72 (m, 3H), 7.65-7.56 (m, 3H), 6.57 (d, J = 8.8 Hz, 2H), 5.68 (s, 2H), 4.64 (br d, J = 5.6 Hz, 1H), 2.42-2.36 (m, 2H), 2.20 (s, 6H), 2.03-1.89 (m, 2H) ppm 234 4-amino-N-[2-[[4-[3-[(3R)-3-hydroxy-1- 452.0 11H NMR (400 MHz, DMSO-d6) δ = 12.35 piperidyl]phenyl]thiazol-2-yl]amino]-2- (s, 1H) 8.48 (m, 1H), 7.58-7.67 (m, 4H), oxo-ethyl]benzamide 7.40 (br s, 1H), 7.31 (m, 1H), 7.02 (m, 1H), 6.64 (d, J = 8.4 Hz, 2H), 4.12 (m, 2H), 3.58 (m, 3H), 2.83 (br s, 1H), 2.68-2.74 (m, 1H), 1.80-1.96 (m, 2H), 1.61 (m, 1H), 1.36 (m, 1H) ppm 235 4-amino-N-[2-oxo-2-[[4-[3-(1- 481.1 1H NMR (400 MHz, DMSO-d6) δ = 12.32 tetrahydropyran-4- (br s, 1H), 8.43 (m, 1H), 7.90 (s, 1H), yloxyethyl)phenyl]thiazol-2- 7.79 (d, J = 7.6 Hz, 1H), 7.66-7.57 (m, 3H), yl]amino]ethyl]benzamide 7.40 (m, 1H), 7.30 (d, J = 7.6 Hz, 1H), 6.57 (d, J = 8.8 Hz, 2H), 5.68 (s, 2H), 4.70 (m, 1H), 4.12 (m, 2H), 3.86-3.68 (m, 2H), 3.41-3.39 (m, 1H), 3.30-3.24 (m, 2H), 1.92 (m, 1H), 1.71-1.61 (m, 1H), 1.50- 1.31 (m, 5H) ppm 236 4-amino-N-[2-[[4-[3-[(3S)-3-hydroxy-1- 452.1 1H NMR (400 MHz, DMSO-d6) δ = 12.33- piperidyl]phenyl]thiazol-2-yl]amino]-2- 12.32 (m, 1H), 8.42-8.39 (m, 1H), 7.62- oxo-ethyl]benzamide 7.58 (m, 3H), 7.47 (s, 1H), 7.28-7.21 (m, 2H), 6.88-6.86(m, 1H), 6.56 (d, J = 6.8 Hz, 2H), 5.66 (s, 2H), 4.85 (d, J = 2.0 Hz, 1H), 4.11 (d, J = 5.6 Hz, 2H), 3.62-3.51 (m, 2H), 2.68-2.66 (m, 1H), 2.71-2.66 (m, 2H), 1.93-1.89 (m, 1H), 1.79-1.74 (m, 1H), 1.59-1.50 (m, 1H), 1.33-1.24 (m, 1H) ppm 237 4-amino-N-[(1S)-1-[[4-[3-[3- 532.3 1H NMR (400 MHz, METHANOL-d4) δ = (aminomethyl)phenyl]phenyl]thiazol-2- 8.21 (s, 1H), 8.09 (d, J = 8.8 Hz, 2H), 7.92 yl]carbamoyl]-3-methylsulfanyl- (d, J = 7.6 Hz, 1H), 7.82 (s, 1H), 7.77 (d, J = propyl]benzamide 8.0 Hz, 1H), 7.64 (d, J = 8.0 Hz, 1H), 7.58-7.46 (m, 6H), 4.95-4.92 (m, 1H), 4.22 (s, 2H), 2.76-2.65 (m, 2H), 2.36- 2.18 (m, 2H), 2.14 (s, 3H) ppm 238 4-amino-N-[2-[[4-[3-[(3S)-3-methoxy-1- 466.0 1H NMR (400 MHz, DMSO-d6) δ = 12.29 piperidyl]phenyl]thiazol-2-yl]amino]-2- (s, 1H), 8.41-8.38 (s, 1H), 7.60 (d, J = 8.4, oxo-ethyl]benzamide 3H), 7.46 (s, 1H), 7.30-7.22 (m, 2H), 6.92-6.89 (m, 1H), 6.56 (d, J = 8.4, 2H), 5.65 (s, 2H), 4.10 (d, J = 5.6, 2H), 3.69-3.65 (m, 1H), 3.47-3.43 (m, 1H), 3.37-3.35 (m, 1H), 3.29 (s, 3H), 2.84-2.77 (1H), 2.73-2.68 (m, 1H), 2.02-1.98 (m, 1H), 1.82-1.77 (m, 1H), 1.57-1.52 (s, 1H), 1.38-1.28 (m, 1H) ppm. 239 4-amino-N-[2-oxo-2-[[4-(3- 437.2 1H NMR (400 MHz, DMSO-d6) δ = 12.36 tetrahydropyran-2-ylphenyl)thiazol-2- (br s, 1H), 8.46 (br s, 1H), 7.91 (br s, 1H), yl]amino]ethyl]benzamide 7.76 (d, J = 7.2 Hz, 1H), 7.65 (s, 2H), 7.59 (s, 1H), 7.36 (m, 1H), 7.24 (d, J = 6.8 Hz, 1H), 6.71-6.51 (m, 2H), 4.34 (d, J = 10.8 Hz, 1H), 4.13 (s, 2H), 4.08-3.98 (m, 2H), 1.96-1.75 (m, 2H), 1.71-1.32 (m, 4H) 1H NMR (400 MHz, DMSO + D2O) δ = 7.88 (br s, 1H), 7.74 (d, J = 7.6 Hz, 1H), 7.65 (d, J = 8.0 Hz, 2H), 7.55 (s, 1H), 7.41- 7.30 (m, 1H), 7.23 (d, J = 7.6 Hz, 1H), 6.70 (d, J = 8.4 Hz, 2H), 4.32 (d, J = 10.8 Hz, 1H), 4.12 (s, 2H), 4.01 (d, J = 11.2 Hz, 1H), 3.59-3.42 (m, 1H), 1.90-1.75 (m, 2H), 1.70-1.35 (m, 4H) 19F NMR (376 MHz, DMSO + D2O) δ = −74.24 (s) 240 4-amino-N-[2-oxo-2-[(4-quinazolin-7- 405.3 1H NMR (400 MHz, DMSO-d6) δ = 9.58 (s, ylthiazol-2-yl)amino]ethyl]benzamide 1H), 9.29 (s, 1H), 8.50 (s, 1H), 8.45 (t, J = 6.0 Hz, 1H), 8.33-8.31 (m, 1H), 8.21 (d, J = 8.4 Hz, 1H), 8.10 (s, 1H), 7.62 (d, J = 8.4 Hz, 2H), 6.56 (d, J = 8.4 Hz, 2H), 5.67 (s, 2H), 4.14 (d, J = 6.0 Hz, 2H) 241 4-amino-N-[2-[[4-[3-[1-(2-amino-2- 490.1 1H NMR (400 MHz, DMSO-d6) δ = 12.34 methyl-propyl)pyrazol-3- (s, 1H), 8.44-8.43 (m, 1H), 8.33 (s, 1H), yl]phenyl]thiazol-2-yl]amino]-2-oxo- 8.00 (br s, 3H), 7.88-7.79 (m, 3H), 7.69- ethyl]benzamide 7.64 (m, 1H), 7.61 (d, J = 8.6 Hz, 2H), 7.50- 7.48 (m, 1H), 6.85 (d, J = 2.2 Hz, 1H), 6.56 (d, J = 8.7 Hz, 2H), 5.66 (br s, 2H), 4.33 (s, 2H), 4.12 (br d, J = 5.7 Hz, 2H), 1.26 (s, 6H)

Example 88. Assay for ATPase Catalytic Activity of BRM and BRG-1

(739) The ATPase catalytic activity of BRM or BRG-1 was measured by the in vitro biochemical assay using ADP-Glo™ (Promega, V9102). The ADP-Glo™ kinase assay is performed in two steps once the reaction is complete. The first step is to deplete any unconsumed ATP in the reaction. The second step is to convert the reaction product ADP to ATP, which will be utilized by the luciferase to generate luminesce and be detected by a luminescence reader, such as Envision.

(740) The assay reaction mixture (10 μL) contains 30 nM of BRM or BRG-1, 20 nM salmon sperm DNA (from Invitrogen, UltraPure™ Salmon Sperm DNA Solution, cat #15632011), and 400 μM of ATP in the ATPase assay buffer, which comprises of 20 mM Tris, pH 8, 20 mM MgCl.sub.2, 50 mM NaCl, 0.1% Tween-20, and 1 mM fresh DTT (Pierce™ DTT (Dithiothreitol), cat #20290). The reaction is initiated by the addition of the 2.5 μL ATPase solution to 2.5 μL ATP/DNA solution on low volume white Proxiplate-384 plus plate (PerkinElmer, cat #6008280) and incubates at room temperature for 1 hour. Then following addition of 5 μL of ADP-Glo™ Reagent provided in the kit, the reaction incubates at room temperature for 40 minutes. Then 10 μL of Kinase Detection Reagent provided in the kit is added to convert ADP to ATP, and the reaction incubates at room temperature for 60 minutes. Finally, luminescence measurement is collected with a plate-reading luminometer, such as Envision.

(741) BRM and BRG-1 were synthesized from high five insect cell lines with a purity of greater than 90%. IP.sub.50 data for compounds 1-241 from the ATPase catalytic activity assay described herein are shown in Tables 5 and 6 below.

(742) TABLE-US-00006 TABLE 5 Compound BRM IP.sub.50 BRM Max % BRG1 IP.sub.50 BRG1 Max % No. (μM)* Inhibition (μM)* Inhibition 1 ++ >95% ++ >70% 2 +++ >95% ++ >95% 3 ++ >95% ++ >90% 4 ++ >90% + >70% 5 + >90% + >70% 6 ++ >90% + >60% 7 ++ >90% ++ >60% 8 ++ >90% + >70% 9 +++ >90% +++ >60% 10 + >90% + >90% 11 ++ >80% + >70% 12 ++ >80% + >60% 13 ++ >80% ++ >30% 14 ++ >80% + >60% 15 ++ >80% ++ >60% 16 ++ >80% + >60% 17 ++ >80% + >60% 18 + >80% + >60% 19 +++ >70% + >30% 20 + >70% + >60% 21 + >70% + >60% 22 + >70% + >60% 23 ++ >70% ++ >50% 24 ++ >70% NT >99% 25 + >70% + >50% 26 + >60% + <30% 27 + >60% + >30% 28 ++ >50% ++ >30% 29 + >50% + >60% 30 NT >70% NT >50% 31 ++ >30% ++ <30% 32 + >30% + <30% 33 + >80% + >80% 34 + >90% + >60% 35 + >95% + >90% 36 +++ >95% ++ >90% 37 + >50% + >30% 38 + >80% + >80% 39 + >30% + <30% 40 NT >50% NT <30% 41 ++ >90% ++ >80% 42 ++ >90% ++ >80% 43 + >80% + >70% 44 + >70% + >70% 45 + NT + NT 46 ++ >60% ++ >70% 47 + >70% + >70% 48 + >50% + >30% 49 ++ >50% ++ >30% 50 + >30% + >30% 51 ++ >90% + >80% 52 +++ >95% +++ >95% 53 ++ >95% ++ >90% 54 ++ >90% ++ >90% 55 ++ >90% ++ >80% 56 + >90% + >80% 57 + >90% + >90% 58 ++ >90% ++ >90% 59 + >90% + >90% 60 + >90% + >80% 61 ++ >80% ++ >50% 62 ++ >80% ++ >70% 63 + >80% + >80% 64 ++ >70% ++ >60% 65 ++ >70% ++ >30% 66 + >70% + >50% 67 + >70% + >70% 68 + >60% + >60% 69 + >60% + >60% 70 + >60% + >50% 71 ++ >60% + >50% 72 + >60% + >50% 73 +++ >50% ++ >30% 74 + >50% + >30% 75 + >50% + >60% 76 + >30% + >30% 77 + >30% + >30% 78 + NT + NT 79 +++ >30% ++ >30% 80 +++ >95% +++ >95% 81 +++ >95% +++ >95% 82 ++ >95% ++ >95% 83 ++ >90% + >80% 84 + >80% + >80% 85 ++ >95% ++ >90% 86 + >70% + >70% “+” indicates inhibitory effect of >10 μM; “++” indicates inhibitory effect of 1-10 μM; “+++” indicates inhibitory effect of <1 μM; “NT” indicates not tested

(743) TABLE-US-00007 TABLE 6 Compound BRM IP.sub.50 BRM Max % BRG1 IP.sub.50 BRG1 Max % No. (μM)* Inhibition (μM)* Inhibition 87 ++++ >95% ++++ >95% 88 +++ >95% ++ >95% 89 ++++ >80% +++ >80% 90 ++++ >70% ++++ >50% 91 +++ >95% ++ >90% 92 +++ >90% +++ >80% 93 +++ >90% +++ >90% 94 +++ >95% ++ >90% 95 ++++ >95% ++++ >95% 96 +++ >70% ++ >70% 97 +++ >70% +++ >70% 98 +++ >90% +++ >70% 99 ++++ >90% ++++ >95% 100 +++ >80% ++ >80% 101 +++ >95% +++ >95% 102 +++ >80% +++ >80% 103 +++ >70% ++ >80% 104 ++++ >70% ++++ >60% 105 +++ >80% +++ >50% 106 +++ >70% +++ >60% 107 +++ >95% ++ >95% 108 +++ >80% +++ >90% 109 +++ >90% +++ >90% 110 +++ >80% ++ >80% 111 +++ >90% +++ >90% 112 +++ >90% +++ >80% 113 +++ >95% +++ >95% 114 +++ >80% ++ >80% 115 +++ >90% +++ >90% 116 +++ >95% +++ >95% 117 ++++ >95% ++++ >95%, 118 +++ >90% ++ >90% 119 +++ >95% ++ >95% 120 +++ >90% +++ >90% 121 +++ >95% +++ >95% 122 +++ >95% ++ >90% 123 +++ >80% +++ >80% 124 +++ >95% +++ >95% 125 ++++ >95% ++++ >95% 126 ++++ >80% ++++ >80% 127 +++ >90% +++ >80% 128 ++++ >95% ++++ >95% 129 +++ >95% +++ >95% 130 +++ >70% ++ >70% 131 ++++ >95% ++++ >95% 132 ++++ >80% ++++ >70% 133 ++++ >95% ++++ >95% 134 +++ >50% +++ >50% 135 ++++ >95% ++++ >95% 136 +++ >95% +++ >95% 137 +++ >90% +++ >95% 138 +++ >90% +++ >90% 139 ++++ >95% ++++ >95% 140 +++ >95% +++ >90% 141 ++++ >95% +++ >95% 142 +++ >90% +++ >90% 143 ++++ >95% ++++ >95% 144 +++ >95% +++ >95% 145 +++ >90% +++ >90% 146 ++++ >95% ++++ >95% 147 ++++ >95% ++++ >95% 148 ++++ >95% ++++ >95% 149 ++++ >80% ++++ >80% 150 ++++ >95% ++++ >90% 151 +++ >80% +++ >80% 152 +++ >90% +++ >80% 153 ++++ >90% ++++ >80% 154 ++++ >95% ++++ >95% 155 +++ >80% +++ >80% 156 +++ >95% +++ >90% 157 ++++ >95% ++++ >90% 158 +++ >90% +++ >95% 159 +++ >90% +++ >95% 160 +++ >95% +++ >95%, 161 +++ >70% +++ >50% 162 ++++ >95% ++++ >95% 163 ++++ >95% +++ >95% 164 ++++ >95% ++++ >95% 165 ++++ >95% ++++ >95% 166 +++ >95% +++ >95% 167 +++ >95% +++ >95% 168 ++++ >95% ++++ >95% 169 +++ >95% +++ >95% 170 +++ >95% +++ >95% 171 +++ >95% +++ >95% 172 +++ >95% +++ >90% 173 +++ >90% +++ >90% 174 +++ >80% +++ >80% 175 +++ >90% +++ >80% 176 +++ >90% +++ >80% 177 ++++ >95% ++++ >95% 178 ++++ >95% ++++ >95% 179 +++ >90% +++ >95% 180 +++ >90% +++ >95% 181 ++++ >95% ++++ >95% 182 ++++ >95% +++ >90% 183 ++++ >95% ++++ >95% 184 ++++ >80% ++++ >70% 185 +++ >80% +++ >70% 186 +++ >95% +++ >95% 187 +++ >70% +++ >60% 188 ++++ >95% ++++ >80% 189 +++ >90% +++ >90% 190 +++ >80% +++ >80% 191 ++++ >95% +++ >95% 192 +++ >90% +++ >90% 193 +++ >80% +++ >90% 194 +++ >90% +++ >80% 195 ++++ >95% ++++ >95% 196 +++ >95% +++ >90% 197 ++++ >90% ++++ >80% 198 +++ >80% +++ >60% 199 +++ >95% +++ >95% 200 ++++ >95% ++++ >95% 201 ++++ >95% ++++ >90% 202 ++++ >95% ++++ >95% 203 +++ >80% +++ >80% 204 +++ >80% +++ >70% 205 ++++ >95% ++++ >95% 206 +++ >95% +++ >95% 207 ++++ >95% ++++ >95% 208 +++ >90% +++ >80% 209 ++++ >95% ++++ >95% 210 +++ >95% +++ >80% 211 +++ >95% +++ >90% 212 +++ >95% +++ >95% 213 ++++ >95% ++++ >95% 214 ++++ >95% ++++ >95% 215 +++ >90% +++ >90% 216 ++++ >90% +++ >95% 217 ++++ >90% ++++ >90% 218 ++++ >95% +++ >90% 219 ++++ >95% ++++ >95% 220 +++ >95% ++ >80% 221 +++ >95% +++ >80% 222 +++ >95% +++ >80% 223 ++++ >95% ++++ >90% 224 ++++ >95% ++++ >90% 225 +++ >80% +++ >90% 226 ++++ >95% ++++ >95% 227 ++++ >95% ++++ >95% 228 +++ >90% +++ >90% 229 +++ >95% +++ >95% 230 +++ >95% +++ >90% 231 +++ >90% +++ >90% 232 +++ >80% +++ >80% 233 +++ >90% +++ >80% 234 +++ >90% +++ >90% 235 +++ >90% +++ >70% 236 +++ >90% +++ >90% 237 +++ >90% +++ >90% 238 +++ >90% +++ >95% 239 +++ >90% +++ >80% 240 +++ >80% +++ >80% 241 +++ >90% +++ >90% “+” indicates inhibitory effect of >10 μM; “++” indicates inhibitory effect of 1-10 μM; “+++” indicates inhibitory effect of 0.1-1 μM, “++++” indicates inhibitory effect of <0.1 μM

Example 89. Effects of BRG1/BRM ATPase Inhibition on the Growth of Uveal Melanoma and Hematological Cancer Cell Lines

(744) Procedure: Uveal melanoma cell lines (92-1, MP41, MP38, MP46), prostate cancer cell lines (LNCAP), lung cancer cell lines (NCIH1299), and immortalized embryonic kidney lines (HEK293T) were plated into 96 well plates with growth media (See Table 7). BRG1/BRM ATPase inhibitor, Compound 87, was dissolved in DMSO and added to the cells in a concentration gradient from 0 to 10 micromolar at the time of plating. Cells were incubated at 37 degrees Celsius for 3 days. After three days of treatment, the media was removed from the cells, and 30 microliters of TrypLE (Gibco) was added to cells for 10 minutes. Cells were detached from the plates, and resuspended with the addition of 170 microliters of growth media. Cells from two DMSO-treated control wells were counted, and the initial number of cells plated at the start of the experiment, were re-plated into fresh-compound containing plates for an additional four days at 37 degrees Celsius. At day 7, cells were harvested as described above. On day 3 and day 7, relative cell growth was measured by the addition of Cell-titer glo (Promega), and luminescence was measured on an Envision plate reader (Perkin Elmer). The concentration of compound at which each cell line's growth was inhibited by 50% (GI.sub.50), was calculated using Graphpad Prism, and is plotted below. For multiple myeloma cell lines (OPM2, MM1S, LP1), ALL cell lines (TALL1, JURKAT, RS411), DLBCL cell lines (SUDHL6, SUDHL4, DB, WSUDLCL2, PFEIFFER), AML cell lines (OCIAML5), MDS cell lines (SKM1), ovarian cancer cell lines (OV7, TYKNU), esophageal cancer cell lines (KYSE150), rhabdoid tumor lines (RD, G402, G401, HS729, A204), liver cancer cell lines (HLF, HLE, PLCRPF5), and lung cancer cell lines (SW1573, NCIH2444), the above methods were performed with the following modifications: Cells were plated in 96 well plates, and the next day, BRG1/BRM ATPase inhibitor, Compound 87, was dissolved in DMSO and added to the cells in a concentration gradient from 0 to 10 micromolar. At the time of cell splitting on days 3 and 7, cells were split into new 96 well plates, and fresh compound was added four hours after re-plating.

(745) Table 7 lists the tested cell lines and growth media used.

(746) TABLE-US-00008 TABLE 7 Cell Lines and Growth Media Cell Line Source Growth Media 92-1 SIGMA RPMI1640 + 20% FBS A204 ATCC McCoy's 5A + 10% FBS DB ATCC RPMI1640 + 10% FBS G401 ATCC McCoy's 5A + 10% FBS G402 ATCC McCoy's 5A + 10% FBS HEK293T ATCC DMEM + 10% FBS HLE JCRB DMEM + 10% FBS HLF JCRB DMEM + 10% FBS HS729 ATCC DMEM + 10% FBS JURKAT ATCC RPMI1640 + 10% FBS KYSE150 DSMZ RPMI1640/Ham's F12 + 10% FBS LNCAP ATCC RPMI1640 + 10% FBS LP1 DSMZ IMDM + 20% FBS MM1S ATCC RPMI1640 + 10% FBS MP38 ATCC RPMI1640 + 20% FBS MP41 ATCC RPMI1640 + 20% FBS MP46 ATCC RPMI1640 + 20% FBS NCIH1299 ATCC RPMI1640 + 10% FBS NCIH2444 ATCC RPMI1640 + 20% FBS OCIAML5 DSMZ alpha-MEM + 20% FBS +10 ng/ml GM-CSF OPM2 DSMZ RPMI1640 + 10% FBS OV7 ECACC DMEM/Ham's F12 (1:1) + 2 mM Glutamine + 10% FBS + 0.5 ug/ml hydrocortisone + 10 ug/ml insulin PFEIFFER ATCC RPMI1640 + 10% FBS PLCPRF5 ATCC EMEM + 10% FBS RD ATCC DMEM + 10% FBS RS411 ATCC RPMI1640 + 10% FBS SKM1 JCRB RPMI1640 + 10% FBS SUDHL4 DSMZ RPMI1640 + 10% FBS SUDHL6 ATCC RPMI1640 + 20% FBS SW1573 ATCC DMEM + 10% FBS TALL1 JCRB RPMI1640 + 10% FBS TYKNU JCRB EMEM + 20% FBS WSUDLCL2 DSMZ RPMI1640 + 10% FBS

(747) Results: As shown in FIG. 1, the uveal melanoma and hematologic cancer cell lines were more sensitive to BRG1/BRM inhibition than the other tested cell lines. Inhibition of the uveal melanoma and hematologic cancer cell lines was maintained through day 7.

Example 90. Comparison of BRG1/BRM Inhibitors to Clinical PKC and MEK Inhibitors in Uveal Melanoma Cell Lines

(748) Procedure: Uveal melanoma cell lines, 92-1 or MP41, were plated in 96 well plates in the presence of growth media (See Table 7). BAF ATPase inhibitors (Compound 87), PKC inhibitor (LXS196; MedChemExpress), or MEK inhibitor (Selumetinib; Selleck Chemicals) were dissolved in DMSO and added to the cells in a concentration gradient from 0 to 10 micromolar at the time of plating. Cells were incubated at 37 degrees Celsius for 3 days. After three days of treatment, cell growth was measured with Cell-titer glow (Promega), and luminescence was read on an Envision plate reader (Perkin Elmer).

(749) Results: As shown in FIG. 2, Compound 87 showed comparable growth inhibition of uveal melanoma cells as the clinical PKC and MEK inhibitors. Further, compound 87 was found to result in a faster onset of inhibition than the clinical PKC and MEK inhibitors.

Example 91. Synthesis of Compound B

(750) BRG1/BRM Inhibitor Compound B has the structure:

(751) ##STR00527##

(752) Compound B was synthesized as shown in Scheme 2 below.

(753) ##STR00528##

Step 1: Preparation of (S)-1-(methylsulfonyl)-N-(4-(methylthio)-1-oxo-1-((4-(3-(pyridin-4-yl)phenyl)thiazol-2-yl)amino)butan-2-yl)-1H-pyrrole-3-carboxamide (Compound B)

(754) ##STR00529##

(755) To a mixture of (2S)-2-amino-4-methylsulfanyl-N-[4-[3-(4-pyridyl)phenyl]thiazol-2-yl]butanamide (2 g, 4.75 mmol, HCl salt) and 1-methylsulfonylpyrrole-3-carboxylic acid (898.81 mg, 4.75 mmol) in DMF (20 mL) was added EDCl (1.37 g, 7.13 mmol), HOBt (962.92 mg, 7.13 mmol) and DIEA (2.46 g, 19.00 mmol, 3.31 mL) and the mixture was stirred at 25° C. for 3 hours. The mixture was poured into H.sub.2O (100 mL) and the precipitate was collected by filtration. The solid was triturated in MeOH (20 mL) and the precipitate was collected by filtration. The solid was dissolved in DMSO (10 mL) and then the mixture was poured into MeOH (50 mL) and the formed precipitate was collected by filtration and lyophilized to give Compound B (2.05 g, 3.66 mmol, 77.01% yield) as a white solid. LCMS (ESI) m/z [M+H].sup.+=555.9. .sup.1H NMR (400 MHz, DMSO) δ 12.49 (s, 1H), 8.68-8.66 (m, 2H), 8.46 (d, J=7.2 Hz, 1H), 8.31-8.30 (m, 1H), 8.02-8.00 (m, 1H), 7.94-7.96 (m, 1H), 7.83 (s, 1H), 7.73-7.74 (m, 3H), 7.61-7.57 (m, 1H), 7.31-7.29 (m, 1H), 6.79-6.77 (m, 1H), 4.74-4.69 (m, 1H), 3.57 (s, 3H), 2.67-2.53 (m, 2H), 2.13-2.01 (m, 5H). SFC: AS-3-MeOH (DEA)-40-3 mL-35T.Icm, t=0.932 min, ee %=100%.

Example 92. Effects of BRG1/BRM ATPase Inhibition on the Growth of Uveal Melanoma, Hematological Cancer, Prostate Cancer, Breast Cancer, and Ewing's Sarcoma Cell Lines

(756) Procedure: All cell lines described above in Example 89 were also tested as described above with Compound B. In addition, the following cell lines were also tested as follows. Briefly, for Ewing's sarcoma cell lines (CADOES1, RDES, SKES1), retinoblastoma cell lines (WERIRB1), ALL cell lines (REH), AML cell lines (KASUMI1), prostate cancer cell lines (PC3, DU145, 22RV1), melanoma cell lines (SH4, SKMEL28, WM115, COL0829, SKMEL3, A375), breast cancer cell lines (MDAMB415, CAMA1, MCF7, BT474, HCC1419, DU4475, BT549), B-ALL cell lines (SUPB15), CML cell lines (K562, MEG01), Burkitt's lymphoma cell lines (RAMOS2G64C10, DAUDI), mantle cell lymphoma cell lines (JEKO1, REC1), bladder cancer cell lines (HT1197), and lung cancer cell lines (SBCS), the above methods were performed with the following modifications: Cells were plated in 96 well plates, and the next day, BRG1/BRM ATPase inhibitor, Compound B, was dissolved in DMSO and added to the cells in a concentration gradient from 0 to 10 micromolar. At the time of cell splitting on days 3 and 7, cells were split into new 96 well plates, and fresh compound was added four hours after re-plating.

(757) TABLE-US-00009 TABLE 8 lists the tested cell lines and growth media used. Cell Line Source Growth Media 22RV1 ATCC RPMI1640 + 10% FBS A375 ATCC DMEM + 10% FBS BT474 ATCC Hybricare medium + 1.5 g/L sodium bicarbonate + 10% FBS BT549 ATCC RPMI1640 + 0.023 IU/ml insulin + 10% FBS CADOES1 DSMZ RPMI1640 + 10% FBS CAMA1 ATCC EMEM + 10% FBS COLO829 ATCC RPMI1640 + 10% FBS DAUDI ATCC RPMI1640 + 10% FBS DU145 ATCC EMEM + 10% FBS DU4475 ATCC RPMI1640 + 10% FBS HCC1419 ATCC RPMI1640 + 10% FBS HT1197 ATCC EMEM + 10% FBS JEKO1 ATCC RPMI1640 + 20% FBS K562 ATCC IMDM + 10% FBS KASUMI1 ATCC RPMI1640 + 10% FBS MCF7 ATCC EMEM + 0.01 mg/ml bovine insulin + 10% FBS MDAMB415 ATCC Leibovitz's L-15 + 2 mM L-glutamine + 10 mcg/ml insulin + 10 mcg/ml glutathione + 15% FBS MEG01 ATCC RPMI1640 + 10% FBS PC3 ATCC F-12K + 10% FBS RAMOS2G64C10 ATCC RPMI1640 + 10% FBS RDES ATCC RPMI1640 + 15% FBS REC1 ATCC RPMI1640 + 10% FBS REH ATCC RPMI1640 + 10% FBS SBC5 JCRB EMEM + 10% FBS SH4 ATCC DMEM + 10% FBS SKES1 ATCC McCoy's 5A + 15% FBS SKMEL28 ATCC EMEM + 10% FBS SKMEL3 ATCC McCoy's 5A + 15% FBS SUPB15 ATCC IMDM + 4 mM L-glutamine + 1.5 g/L sodium bicarbonate + 0.05 mM 2-mercaptoethanol + 20% FBS WERIRB1 ATCC RPMI1640 + 10% FBS WM115 ATCC EMEM + 10% FBS

(758) Results: As shown in FIG. 3, the uveal melanoma, hematologic cancer, prostate cancer, breast cancer, and Ewing's sarcoma cell lines were more sensitive to BRG1/BRM inhibition than the other tested cell lines. Inhibition of the uveal melanoma, hematologic cancer, prostate cancer, breast cancer, and Ewing's sarcoma cell lines was maintained through day 7.

Other Embodiments

(759) While the invention has been described in connection with specific embodiments thereof, it will be understood that invention is capable of further modifications and this application is intended to cover any variations, uses, or adaptations of the invention following, in general, the principles of the invention and including such departures from the present disclosure that come within known or customary practice within the art to which the invention pertains and may be applied to the essential features hereinbefore set forth, and follows in the scope of the claims.

(760) Other embodiments are in the claims.