ACTIVATED MICRO-BUBBLE BASED ROOT CANAL DISINFECTION SYSTEM

Abstract

A photoactive solution and a micro-bubble solution are provided as part of a system for use in disinfecting a root canal. The system also include a handpiece having a flexible tip. When the handpiece is energized, vibrations are induced in the flexible tip to mechanically activate the micro-bubble solution in the root canal. The photoactive solution contains a photoactive compound dissolved in an alcohol carrier solution; and the micro-bubble solution contains an oxygen carrier, an oxidizing agent and a surfactant. The flexible tip transmits light energy into the root canal. The micro-bubble solution facilitates delivery of the light to the photoactive solution to activate the photoactive solution in the canal. The activated photoactive solution then acts on the oxygen introduced in the canal via the micro-bubble solution to disinfect the root canal.

Claims

1. A root canal disinfecting system comprising a photosensitive solution and a separate oxygen carrying, micro-bubble producing solution; the photosensitive solution comprising a photoactive compound dissolved in an alcohol carrier solution; wherein, the photosensitive compound is chosen from the group consisting of toluidine blue (TBO), methylene blue (MB), rose bengal (RB) arianor steel blue, tryptan blue, crystal violet, azure blue cert, azure B chloride, azure 2, azure A chloride, azure B tetrafluoroborate, thionin, azure A eosinate, azure B eosinate, azure mix sicc, azure II eosinate, haematoporphyrin HCl, haematoporphyrin ester, aluminum disulphonated phthalocyanine, chlorins, photoactive fullerenes (e.g. Cl6-b), aminolevulinic acid (ALA), bacteriochlorins, phthalocyanines, pheophorbides, purpurins, naphthalocyanines, indocyanine green, and combinations thereof; and the alcohol solution comprises polyethylene glycol and/or ethanol; and the oxygen carrying, micro-bubble producing solution comprising an oxygen carrier, an oxidizing agent and a surfactant; wherein. the oxygen carrier is chosen from the group consisting of perfluorodecahydro naphthalene, perfluorodecalin, perfluorohexane, octafluoropropane, perfluorobutane, perfluorooctane, perfluoromethyldecalin and O.sub.2IrCl(CO)P[C.sub.6H.sub.5].sub.3).sub.2, and combinations thereof; the oxidizing agent of the micro-bubble solution is chosen from the group consisting of hydrogen peroxide (H.sub.2O.sub.2), dilute sodium hypochlorite, dimethyl sulfoxide and chlorine dioxide and combinations thereof; and the surfactant of the micro-bubble solution is chosen from the group consisting of mineral oil, glycerol, polyethylene glycol, non-ionic detergent, polypropylene glycol, SDS, a nonionic polyoxyethylene surfactant and combinations thereof.

2. The root canal disinfecting system of claim 1 further comprising a mechanical activator; said mechanical activator comprising an energizable handpiece having a flexible activator depending from a head of said handpiece; said activator being sized to be inserted in a root canal; whereby, when said handpiece is energized, vibrations are induced in said activator.

3. The root canal disinfecting system of claim 2 wherein said activator is comprised of a fiber optic and said system further includes a light source; said fiber optic transmitting light from said light source along the length of said fiber optic.

4. The root canal disinfecting system of claim 3 wherein said light source is an LED or a laser.

5. A photosensitive solution for use in light activated disinfection of a prepared canal; the solution comprising a photoactive compound dissolved in an alcohol carrier solution, said photosensitive solution being void of oxygen carrying compounds: wherein the photosensitive compound is chosen from the group consisting of toluidine blue (TBO), methylene blue (MB), rose bengal (RB) arianor steel blue, tryptan blue, crystal violet, azure blue cert, azure B chloride, azure 2, azure A chloride, azure B tetrafluoroborate, thionin, azure A eosinate, azure B eosinate, azure mix sicc, azure II eosinate, haematoporphyrin HCl, haematoporphyrin ester, aluminum disulphonated phthalocyanine, chlorins, photoactive fullerenes (e.g. Cl6-b), aminolevulinic acid (ALA), bacteriochlorins, phthalocyanines, pheophorbides, purpurins, naphthalocyanines, indocyanine green, and combinations thereof; and wherein alcohol solution comprises polyethylene glycol and/or ethanol.

6. The solution according to any of claim 5 wherein the photosensitive compound is methylene blue or rose bengal arianor steel blue.

7. The solution according to any of claim 6 wherein the photosensitive compound is methylene blue.

8. The solution according to claim 5 wherein the carrier alcohol solution comprises polyethylene glycol and ethanol.

9. The solution according to claim 5 wherein the polyethylene glycol is glycerol.

10. The solution according to claim 5 wherein the polyethylene glycol, ethanol, and water of the carrier solution are combined in a ratio of about 1:1:1 to about 3:1:2.

11. The solution according to claim 5 wherein the polyethylene glycol, ethanol, and water of the carrier solution are combined in a ratio of about 30:20:50.

12. The solution according to any of claim 5 wherein the photosensitive compound has a concentration of about 5 micro molar to about 100 micro molar in the photosensitive solution.

13. The solution according to any of claim 5 wherein the photosensitive compound has a concentration of about 100 micro molar.

14. An oxygen carrying, micro-bubble producing solution for use in light assisted photodynamic root canal therapy comprising an oxygen carrier, an oxidizing agent and a surfactant, said micro-bubble solution being void of photosensitive compounds; wherein the oxygen carrier is chosen from the group consisting of perfluorodecahydro naphthalene, perfluorodecalin, perfluorohexane, octafluoropropane, perfluorobutane, perfluorooctane, perfluoromethyldecalin and O.sub.2IrCl(CO)P[C.sub.6H.sub.5].sub.3).sub.2, and combinations thereof; wherein the oxidizing agent of the micro-bubble solution is chosen from the group consisting of hydrogen peroxide (H.sub.2O.sub.2), dilute sodium hypochlorite, dimethyl sulfoxide and chlorine dioxide and combinations thereof; and wherein the surfactant of the micro-bubble solution is chosen from the group consisting of mineral oil, glycerol, polyethylene glycol, non-ionic detergent, polypropylene glycol, SDS, a nonionic polyoxyethylene surfactant and combinations thereof.

15. The solution according to claim 14 wherein the oxygen carrier of the micro-bubble solution is a perflourocarbon.

16. The solution according to claim 14 wherein the oxidizing agent of the micro-bubble solution is of hydrogen peroxide (H.sub.2O.sub.2).

17. The solution according to claim 16 wherein the concentration of the hydrogen peroxide (H.sub.2O.sub.2) used is about 35% H.sub.2O.sub.2.

18. The solution according of claim 14 wherein the surfactant of the micro-bubble solution is a nonionic polyoxyethylene surfactant.

19. The solution according to claim 14 wherein the oxygen carrier, oxidizing agent and surfactant of the micro-bubble solution are combined in a ratio of about 30:20:50 by volume.

20. The solution according to claim 14 wherein the oxygen carrier, oxidizing agent and surfactant of the micro-bubble solution are combined in a ratio of 75.0:24.5:0.5 by volume.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0033] FIG. 1 is a schematic representation of root canal disinfection via photo-chemically activated micro-bubbles;

[0034] FIG. 2 contains photographs of tooth cross-sections showing diffusion into dentin of a photosensitizing compound (Methylene Blue) when dissolved in (1) water, (2) glycerol (GLY), (3) polyethylene glycol (PEG) and (4) a solution of water, glycerol and polyethylene glycol (MIX);

[0035] FIG. 3 is a photograph of a prototype activator used to both mechanically activate the micro-bubble solution and to introduce light into the root canal;

[0036] FIG. 4 contains enlarged photographs of the fiber optic activator in the micro-bubble solution in an unactivated state (left side) and in an activated state (right side);

[0037] FIG. 5 contains images demonstrating the penetration of light from the fiber optic cable into the dentin due to activation of the micro-bubble solution;

[0038] FIG. 6 is a graph comparing singlet oxygen release from methylene blue dissolved in (1) water, (2) glycerol, (3) polyethylene glycol and (4) a solution of water, glycerol and polyethylene glycol (MIX);

[0039] FIG. 7 is a graph showing the rate of singlet oxygen production in the micro-bubble solution with methylene blue dissolved in different ratios of perfluorocarbon (PFC), hydrogen peroxide, and water, wherein: [0040] PF1 comprises 50 ?mol/L of MB in combination with perfluorodecahydronaphthalene; [0041] PF2 comprises 50 ?mol/L of MB in combination with perfluorodecahydronaphthalene and H.sub.2O.sub.2 in a 66.6:33.3 ratio; [0042] PF3 comprises, 50 ?mol/L of MB in an emulsion produced by mixing perfluorodecahydronaphthalene:H.sub.2O.sub.2:Triton-X100 in a 60:35:5 ratio; and [0043] PF4 comprises 50 ?mol/L of MB in an emulsion produced by mixing perfluoroecahydronaphthalene:H.sub.2O.sub.2:triton-X100 in a 75:24.5:0.5 ratio. PF-4 is demonstrated to produce maximum singlet oxygen release;

[0044] FIG. 8 is a schematic of fluid dynamics in a root canal when an antibacterial liquid (irrigant) is delivered using syringe irrigation;

[0045] FIG. 9 contains a graph showing the effect of fluid activation on the penetration of fluid into lateral canals of a root canal;

[0046] FIG. 10 contains photomicrographs (on the right) and a schematic drawing (on the left) showing the micro-bubbles that are produced in a root canal upon sonic/ultrasonic activation of the micro-bubble solution in the canal;

[0047] FIG. 11 contains graphs showing singlet oxygen yield of methylene blue (the left hand graph) and rose bengal (the right hand graph) both with and without sonic activation;

[0048] FIG. 12 contains a photomicrograph and colored cross-sectional photograph of immature (4-day old) biofilm on a root canal wall;

[0049] FIG. 13 contains images of a mature (6 week old) bio-film;

[0050] FIG. 14 is a schematic that graphically demonstrates the strategy of photoenhanced activated micro-bubble based root canal disinfection;

[0051] FIG. 15 contains in the first two columns, images showing the three-dimensional confocal laser scanning microscopy reconstruction of the Enterococcus faecalis biofilms subjected to different treatments (60?). The left column shows the biofilm receiving no treatment (top), methylene blue (photosensitizer) (100 ?mol/L) treatment (middle) and PF4 micro-bubble solution treatment (bottom); the middle column shows the biofilm subjected to light (660 nm) irradiation alone (top), methylene blue+light (660 nm) irradiation (middle) and PF4 micro-bubble solution treatment+light (660 nm); and the right column shows the sagittal section of the treatment groups as described in the middle column MB;

[0052] FIG. 16 contains scanning electron photomicrographs of untreated root canals showing the biofilm on the root canal surface;

[0053] FIG. 17 contains scanning electron photomicrographs of root canals treated via conventional light activated disinfection (i.e., photoactivation of methylene blue dissolved in water) without activation; the remnant smear layer, debris and bacteria can be observed on the root canal walls;

[0054] FIG. 18 contains scanning electron photomicrographs of root canals treated via sonically activated PDT treatment after using the methylene blue dissolved in water;

[0055] FIG. 19 contains scanning electron photomicrographs of root canals treated via photoactivation of methylene blue in water, without use of the micro-bubble solution; although the smear layer and bacterial reduction was marginally better than the group of FIG. 17, the root canal wall still showed significant smear layer and adherent bacterial biofilm;

[0056] FIG. 20 contains scanning electron photomicrographs of root canals treated via photoactivation of methylene blue in water, ethanol and polyethylene glycol in the presence of activated micro-bubble solution; in these photomicrographs, the root canal surface is devoid of smear layer, debris and any biofilm structures;

[0057] FIGS. 21-22 show laser confocal microscopy images showing bacterial biofilms (and dead cells as red and viable cells are green). These images are of a root canal from a control group (i.e., untreated) (FIG. 21), a root canal disinfected via photoactivation of methylene blue in water in the presence of mechanically activated micro-bubble solution (FIG. 22);

[0058] FIG. 23 describes the different mechanisms of the micro-bubble solution for root canal disinfection. When mechanically activated, for example, with ultrasonic/sonic frequency agitation, the micro-bubble solution will activate different mechanisms in the root canal. (1) The bubbles generated by agitation of the liquid and the micro-bubbles in the solution interacting producing significant and bubble-root canal wall interaction, which should be responsible for the physical/mechanical effect of micro-bubbles on debridement and biofilm disruption; (2) The hydrogen peroxide in the micro-bubble solution should interact with the organic tissue debris within the root canal leading to the formation of oxygen, which causes the micro-bubbles to grow further and propel towards the root canal wall (the location of tissue remnants) to further improve debridement and antibiofilm effect as shown in FIGS. 24 and 25; and

[0059] FIGS. 24 and 25 each comprise three images showing micro-bubbles being propelled toward the root canal surface and coalescing on the root canal surface.

[0060] Corresponding reference numerals will be used throughout the several figures of the drawings.

DETAILED DESCRIPTION OF THE INVENTION

[0061] The following detailed description illustrates the invention by way of example and not by way of limitation. This description will clearly enable one skilled in the art to make and use the claimed invention, and describes several embodiments, adaptations, variations, alternatives and uses of the claimed invention, including what I presently believe is the best mode of carrying out the claimed invention. Additionally, it is to be understood that the claimed invention is not limited in its application to the details of construction and the arrangements of components set forth in the following description or illustrated in the drawings. The claimed invention is capable of other embodiments and of being practiced or being carried out in various ways. Also, it is to be understood that the phraseology and terminology used herein is for the purpose of description and should not be regarded as limiting.

[0062] My disinfection method is shown schematically in FIG. 1. Briefly, the root canal disinfection method initially involves preparing the canal. This can be accomplished using conventional cleaning and shaping tools, such as files, reamers, etc. After the root canal has been shaped and cleaned, the canal is rinsed with a photosensitive solution, comprising a photosensitive compound dissolved in a carrier solution. The carrier solution enables the photosensitive compound to penetrate the dentin up to a distance of 1-2 mm, and the photosensitive compound will adhere to the biofilm and bacteria in the biofilm throughout the root canal system (including in microtubules). To facilitate penetration of the dentin by the photosensitive compound, the root canal is flushed with (rather than simply soaked in) the photosensitive solution. Flushing/application with the photosensitive solution can be for about 60-600 seconds, and preferably about 60 to about 300 seconds. Excess photosensitive solution is then removed from the canal using, for example, paper points. Importantly, after removal of the excess photosensitive solution, there is still a thin layer of photosensitizing solution in the canal that has bound to the canal surfaces and to the biofilms/tissue remnants in the canal.

[0063] After excess photosensitizing solution has been withdrawn from the canal, the canal is filled with a micro-bubble solution which is comprised of an oxidizing agent, an oxygen carrier and a surfactant. The surfactant can be neutral, anionic or cationic. The micro-bubble solution is then mechanically activated (for example sonically or ultrasonically, in the canal, and light is introduced into the canal. For example, the solution can be mechanically activated using an EndoActivator? driver and tip which is available from Dentsply Tulsa Dental. Preferably, the tool used to mechanically activate the micro-bubble solution also introduces light into the canal to achieve mechanically activated photodynamic therapy. A prototype device is shown in FIG. 3 in which a fiber optic thread is secured to the head of an EndoActivator? driver to be sonically driven. Activation of the micro-bubble solution generates dissolved oxygen bubbles (and causes or induces acoustic microstreaming) in the canal. These bubbles will interact with and produce significant bubble/canal wall interaction (micro-bubbles bouncing from the walls of the canal), which mechanically disrupts the biofilm, and removes debris and smear layer from the root canal wall. Additionally, the micro-bubbles scatter the light introduced into the canal, so that the light does not merely pass axially through the canal. The light will impact, and activate, the photosensitive compound throughout the root canal system, including photosensitive compound that is adhered to biofilm and/or bacteria in dentinal tubules, lateral canals, etc. As shown schematically in FIG. 1, the activated photosensitive compound releases energy to the oxygen molecules, converting the oxygen molecules to singlet oxygen (S1 or 1P*). The singlet oxygen is highly reactive, and destroys the biofilm and bacteria via oxidative damage.

[0064] The micro-bubble solution can be mechanically activated in the canal for about 60 to about 600 seconds, and preferably about 60 to about 180 seconds. As will be discussed more fully below, this method disrupts and destroys the biofilm, resulting in a canal that is substantially free of biofilm, as shown in FIGS. 20 and 22. Additionally, as will be discussed below, the photosensitive compound reaches uninstrumented portions of the canal, and via the photo-chemically enhanced micro-bubble activated disinfection, destroys bacteria in these uninstrumented areas of the canal. This method thus has applicability to minimally invasive endodontic (MIE) procedures or treatment.

[0065] Photosensitive Solution

[0066] The photosensitive solution comprises a photosensitive compound, which is dissolved in a carrier solution. The photosensitive compound can be one or more of the following: toluidine blue (TBO), methylene blue (MB), rose bengal (RB), arianor steel blue, tryptan blue, crystal violet, azure blue cert, azure B chloride, azure 2, azure A chloride, azure B tetrafluoroborate, thionin, azure A eosinate, azure B eosinate, azure mix sicc, azure II eosinate, haematoporphyrin HCl, haematoporphyrin ester, aluminum disulphonated phthalocyanine, chlorins, photoactive fullerenes (e.g. CI6-b), aminolevulinic acid (ALA), bacteriochlorins, phthalocyanines, pheophorbides, purpurins, naphthalocyanines, indocyanine green, or mixtures thereof. A preferred photosensitive compound is methylene blue (MB).

[0067] The photosensitive solution is described in US 2011/0027384 and US 2009/0285766, both of which are incorporated herein by reference. The carrier solution in which the photosensitizing compound is dissolved can be water or it can be an alcohol solution. Alcohol solutions tend to enable the photosensitive compound to penetrate more deeply into the dentin, and hence, the carrier solution is preferably an alcohol solution. The alcohol solution can comprise polyethylene glycol and/or ethanol. In a preferred embodiment, the photosensitizing compound carrier solution comprises a polyethylene glycol, an ethanol and water. The polyethylene glycol can be glycerol. The polyethylene glycol, alcohol and water can be combined in a ratio of about 1:1:1 to about 3:1:2 by volume. In a preferred embodiment, the polyethylene glycol, alcohol and water are combined in a ratio of 30:20:50 (or 3:2:5) by volume. The ratio 30:20:50 by volume was arrived at by adding the refractive indices of the components so that the final mixture has a refractive index close to that of dentin and at the same time had the ability to penetrate into the dentinal tubules. These features help the polyethylene glycol, alcohol and water mixture achieve better antimicrobial PDT in dentin tissue.

[0068] The ethanol used to prepare the carrier solution can be about 30% to about 100% ethanol. Preferably, the ethanol is of a concentration which when mixed with the polyethylene glycol and water produces a mixture that is about 30% ethanol. The photosensitizing compound in the photosensitizing solution is present in a concentration of less than about 100 micro-molar, and preferably between about 2 micro-molar to about 100 micro-molar. When the concentration of the photosensitizing compound in the photosensitizing solution exceeded 100 micro molar, there was marked aggregation of the photosensitizing compound in the photosensitizing solution, which impaired photodynamic effect. However, the degree of singlet oxygen release is proportional to concentration or the photosensitizing compound. Hence, to maintain the singlet oxygen release as high as possible, the preferred concentration of photosensitizing compound in the photosensitizing solution is about 100 micro-molar. (George S, Kishen A., Photophysical, photochemical, and photobiological characterization of methylene blue formulations for light-activated root canal disinfection, J Biomed Opt. 2007 May-June; 12(3):034029.)

[0069] The carrier solution helps the photosensitizing compound better penetrate the dentinal tubules and anatomical complexities of the canal. It also aids in penetration of the photosensitizing compound into bacterial cells/biofilm structures. FIG. 2 shows a comparison of the penetration of different photosensitizing solutions as follows: (1) methylene blue (MB) in water, (2) MB in glycerol (Gly), (3) MB in polyethylene glycol (PEG), and (4) MB in a water/glycerol/PEG solution (MIX). In each of these four photosensitive solutions, the methylene blue was present in a concentration of 100 micro-molar.

[0070] As can be seen from FIG. 2, the MB had very little penetration into the dentin when mixed only in water. The penetration was better for Gly and PEG. However, as seen, the penetration of the MB into the dentin was substantially better when the MB was dissolved in the polyethylene glycol, ethanol and water mixture. In the PEG/ethanol/water carrier solution, the methylene blue penetrated up to 2 mm into the dentin. This substantial penetration of the photosensitizing compound into the dentin enables the photosensitizing compound to contact and adhere to biofilm deep with the anatomical complexities of the root canal. As will become more apparent, this is one of the features of my method that enables minimally invasive endodontics.

[0071] Micro-Bubble Solution

[0072] The micro-bubble solution is described in US 2011/0027384 and US 2009/0285766, both of which are incorporated herein by reference. Preferably, the micro-bubble solution comprises at least one oxygen carrier, at least one oxidizing agent and at least one surfactant.

[0073] The oxygen carrier can be one or more of the following: perfluorodecahydro naphthalene, perfluorodecalin, perfluorohexane, octafluoropropane, perfluorobutane, perfluorooctane, perfluoromethyldecalin and O.sub.2IrCl(CO)P[C.sub.6H.sub.5].sub.3).sub.2. The preferred oxygen carrier is a perfluorocarbon (PFC).

[0074] The oxidizing agent can be one or more of the following: hydrogen peroxide (H.sub.2O.sub.2), dilute sodium hypochlorite, dimethyl sulfoxide (DMSO) and chlorine dioxide. The preferred oxidizing agent is hydrogen peroxide (H.sub.2O.sub.2).

[0075] The surfactant can be one or more of the following: mineral oil, glycerol, polyethylene glycol, non-ionic detergent, polypropylene glycol, sodium dodecyl sulfate (SDS), a nonionic polyoxyethylene surfactant (such as Triton? X or Triton? X-100), or an antibacterial detergent (such as cetrimide, a mixture of different quaternary ammonium salts including Cetrimonium bromide). In particular, the nonionic detergent may be Triton? X, and even more preferably, Triton? X-100 (available from Sigma-Aldrich). Triton X-100 is a nonionic polyoxyethylene surfactant, having a specific gravity of 1.065 at 25? C. (about 1.07 g/mL), an approximate molecular weight of 625 (giving an effective molarity of 1.7 M for the neat liquid), a UV absorption of lambda max=275 nm and 283 nm in methanol, a viscosity (Brookfield): 240 cps at 25? C., a pH (5% aqueous solution) of 6.0 to 8.0, and critical micelle concentration (CMC) of 0.22 to 0.24 mM. It is soluble in all proportions at 25? C. in water, benzene, toluene, xylene, trichloroethylene, ethylene glycol, ethyl ether, ethanol, isopropanol, and ethylene dichloride. Triton X-100 has the following formula:

##STR00001##

where n is about 9.5

[0076] The oxygen carrier, oxidizing agent and surfactant are combined in a ratio of about 60:35:5 to about 75:24.5:0.5 by volume. In a preferred embodiment, the oxygen carrier, oxidizing agent and surfactant are combined in a ratio of 75.0:24.5:0.5 by volume.

[0077] Micro-bubbles released from the micro-bubble solution are anionic, gas-filled bubbles having a size of about 2-4 microns. The gas core of the micro-bubbles contains most of the unit volume. Micro-bubbles in aqueous media are inherently unstable owing to surface tension effects, and therefore require a stabilizing shell. The surfactant of the carrier solution thus serves to stabilize the micro-bubbles.

[0078] Activation of the Micro-Bubble Solution

[0079] The micro-bubble solution, as noted above, is mechanically activated, for example, either sonically or ultrasonically, in the prepared tooth root canal. Additionally, as the micro-bubble solution is being agitated, light is introduced into the canal, for example, by means of a fiber optic cable. In fact, the fiber optic cable can be sonically or ultrasonically driven in the root canal. Light can be delivered into the canal using a fiber optic cable, and the light source can be an LED, a laser, or other light source. Preferably, the light source is a laser. The source (type) of the laser depends upon the type of photosensitizer used in the micro-bubble solution. In tests, methylene blue was used as a photosensitizer, hence the light source wavelength was 660 nm. The power of the laser tested ranged from 22 mW to 100 mW. Since the effectiveness of photodynamic effect depends on the dose of light, which is directly related to the power of the light source and duration of illumination, for a lower power source, longer duration of illumination can be used. Light doses ranging from 2 J/cm.sup.2 to 60 J/cm.sup.2 for about 60 to about 180 seconds was tested. Preferably, the fiber optic cable has no coating at the end which is received in the root canal to enable light to escape from the cable both through the sides (radially) and from the end (axially) of the cable. Such fiber optic cables are shown, for example, in FIGS. 3 and 4. FIG. 5C schematically shows light escaping from the fiber optic cable both axially and radially. As can be appreciated, the fiber optic cable is sized to extend to the working end of the prepared root canal, and thus has a diameter of about 0.5 mm or less.

[0080] In a preferred method, the fiber optic cable is used to mechanically activate the micro-bubble solution. In FIG. 3, the fiber optic cable is mounted to an irrigant activator, such as an EndoActivator? driver (available from Dentsply Tulsa Dental), and which is described in U.S. Pat. Nos. 7,261,561, 8,235,719, 8,328,552, and 8,388,345 all of which are incorporated herein by reference. This modified EndoActivator? driver was used in the testing of my method. FIG. 4 shows, on the right, sonic activation of the micro-bubble solution in a clear vial using a fiber optic cable in which the end of the cable is not coated (i.e., is stripped of its coating). The left hand picture shows the fiber optic cable in the micro-bubble solution. The light emanating from the cable can be seen to be picked up by the micro bubbles. As can also be seen in the left hand picture, there is little to no activity in the solution. The right hand picture shows the fiber optic cable sonically activating the micro-bubble solution. A high degree of activity is shown around the tip of the cable itself. However, activity can also be seen at the wall of the vial in which the test is conducted. This demonstrates that sonic activation will produce activity at the root canal wall.

[0081] Numerical modeling of the penetration of light into dentin is shown schematically in FIG. 5. In the schematics A-C of FIG. 5, the converging bold lines mark the edges of the dentin, and thus of the shaped canal. (Klein N, Kishen A, Foth H J. Root Canal Disinfection by Photodynamic Inactivation of Bacteria: Light Propagation in Root Canal, Gordon Research Conference on Lasers in Lasers in Medicine & Biology, Holderness, N H, Jul. 22-27, 2012). FIG. 5A schematically shows the penetration of light in a canal without the micro-bubble solution. As seen, the light emanating from the end of the fiber optic passes generally axially through the canal, and very little of the light even reaches the canal wall. FIG. 5B shows the canal with the micro-bubble solution. As seen, the light is scattered and reaches the canal walls. However, the light reaches the canal walls only in the vicinity of the end of the fiber optic cable. FIG. 5C shows the effect of activating the micro-bubble solution on light penetration. As seen, the light is scattered; it reaches axially well beyond the end of the fiber optic cable; and, the light penetrates well into the dentin to reach photosensitizing compound in the dentinal tubules and lateral canal. Because the light penetrates the dentin, the light will reach the photosensitive compound adhered to biofilm and bacteria within the dentin (i.e., in dentinal tubules) in addition to biofilm and bacteria in the main canal and lateral canals, to activate the photoactive compound within the dentinal tubules, lateral canals, and all other aspects of the root canal system.

[0082] As noted above, when the light reaches the photosensitive compound, the photosensitive compound releases energy to convert the oxygen molecules to singlet oxygen. As noted above, H.sub.2O/Gly/PEG as the photosensitizing compound carrier solution substantially enhances the penetration of the photosensitizing compound into the dentin of the root canal. It also substantially increases the amount of oxygen singlet released. FIG. 6 is a chart showing the amount of singlet oxygen released during agitation of the micro-bubble solution in the root canal for four different carrier solutions: (1) water; (2) Glycerol, (3) PEG, and (4) a H.sub.2O/Gly/PEG mixture (MIX). The singlet oxygen release was measured using 1, 3-diphenylisobenzofuran (DPBF). DPBF is a chemical used to study the degree of release of singlet oxygen; it is not actually part of the micro-bubble solution or the photosensitizing solution. As is known, DPBF undergoes bleaching with singlet oxygen and this can be calorimetrically quantified. FIG. 7 shows increased concentration of DPBF, and thus increased concentration of oxygen singlets for varying photosensitizing solution formulations. In this test, four different photosensitizing formulations in micro-bubbles were tested for oxidation potential/singlet oxygen generation as follows: [0083] Solution PF1 comprises 50 micro mol/L of MB in combination with perfluorodecahydronaphthalenel; [0084] Solution PF2 comprises 50 micro mol/L of MB in combination with perfluorodecahydronaphthalene and H.sub.2O.sub.2 (66.6:33.3); [0085] Solution PF3 comprises 50 micro mol/L of MB in an emulsion produced by mixing perfluorodecahydronaphthalene, H.sub.2O.sub.2, and Triton X-100 in the ratio 60:35:5; and [0086] Solution PF4 comprises 50 micro mol/L of MB in an emulsion produced by mixing perfluorodecahydronaphthalene, H.sub.2O.sub.2, and TritonX100 in the ratio 75:24.5:0.5.

[0087] It was found that with solution PF4 there was a significantly greater amount of singlet oxygen generation. Biofilm disruption is proportional to the amount of singlet oxygen released and/or the penetration of photosensitizer into the biofilm structure. Thus, the enhanced singlet oxygen generation lead to a better disruption of matured bacterial biofilm.

[0088] Importantly, the micro-bubble solution is mechanically (for example, sonically or ultrasonically) activated in the canal. This is to be compared with, for example, syringe irrigation. The fluid dynamics of syringe irrigation is shown schematically in FIG. 8. As shown, irrigation fluid is ejected from the end of the syringe. The flow of fluid adjacent the syringe is generally laminar. There is a small area of turbulent flow below the end of the syringe. However, due to the coronally directed reflux near the canal walls, much of this turbulence does not reach the canal wall. This results in a zone of active fluid circulation above the end of the syringe, and a zone of passive fluid circulation below the end of the syringe. Because of this, syringe irrigation does not induce significant stresses on the root canal wall. Therefore, solutions irrigated via syringe irrigation are not forced against the canal wall, and thus the irrigating solution will not penetrate very far into the dentin. Sonic and ultrasonic activation, on the other hand, produces lateral displacement of the fluid adjacent the tip, and causes fluid interaction with the canal wall. The graph of FIG. 9 shows the lateral penetration of sodium hypochlorite into the simulated lateral canals of root dentin and shows that sonic activation dramatically increases the number of lateral canals that are successfully penetrated. Similar synergistic effect is also observed with ultrasonic activation. It is observed that sonic activation of sodium hypochlorite with/without EDTA resulted in significant (numbers and magnitude) penetration into lateral canal.

[0089] Micro-Bubble Solution Dynamics

[0090] My disinfection method allows for the combination of the physical benefits of sonic agitation (physically displacing fluid/micro-bubbles towards the walls of the root canal) and the antibacterial effect of PDT (produced by singlet oxygen release). Larger and deeper penetration of singlet or reactive oxygen aids in marked elimination of biofilm bacteria.

[0091] When the micro-bubble solution is mechanically activated (sonically or ultrasonically) in the canal, both inertial (cavitational) and non-inertial bubble dynamics are produced in the canal. The inertial bubbles (which result from cavitation) will collapse and release energy resulting in heat and shear forces in the canal. These shear forces produce a hydrodynamic effect. The non-inertial (non-cavitational) bubbles oscillate in the fluid, and thus do not collapse, as do the inertial (cavitational) bubbles. These non-inertial bubbles move rapidly around mechanically activated files or tips and produce shear forces that are capable of dislodging materials (i.e., the biofilm) from the canal wall.

[0092] During sonic or ultrasonic activation of the micro-bubble solution, the gas cores of the bubbles expand during the rarefaction phase of the pressure wave and contracts during the compression phase. Enhanced micro-bubble/micro-bubble interaction also occurs during the ultrasonic/sonic activation of micro-bubble solution. This will increase the antibacterial effect of the photodynamic therapy. The higher antibacterial/anti-biofilm effect of photodynamic therapy is explained by the availability of oxygen carrier/oxidizing agent in the micro-bubbles and the activation energy supplied by the ultrasonic/sonic agitation. The above factors will lead to the marked production of singlet/reactive oxygen species, which are responsible for biofilm disruption, which is shown by the graphs of FIGS. 6 and 7.

[0093] The sonic or ultrasonic agitation/activation of the micro-bubble solution in the root canal results in the generation of anionic, stable and transient micro-bubbles. These micro-bubbles, during ultrasonic/sonic agitation, will yield improved micro-bubble/root canal wall interaction (physical effects of root canal disinfectant). It is known that the ultrasonic/sonic assisted irrigation produced the highest wall shear stress on the root canal wall and the highest turbulence intensity of fluid flow coronally from the ultrasonic/sonic tip. Thus the lateral movement of the micro-bubbles displayed has an important implication to enhance the physical effect of stable micro-bubbles to disrupt root canal biofilms.

[0094] FIG. 10 contains photomicrographs and a schematic drawing showing the micro-bubbles that are produced in a root canal upon sonic/ultrasonic activation of the micro-bubble solution in the canal. The oxidizing agent and the oxygen carrier, when activated, produce a significant amount of oxygen, which is released as the micro bubbles in the root canal. The micro-bubble interaction, as noted previously, enhances the photodynamic effect of the PDT. When the micro-bubble solution is mechanically activated, there is an interaction between the stable (anionic) bubbles and the canal wall. This micro-bubble/canal wall interaction generates electrostatic and physical/mechanical shear stresses which mechanically disrupt the biofilm. The mechanically activated micro-bubble solution thus 3-dimensionally improves the physical/mechanical effect within the root canal.

[0095] FIG. 11 contains graphs showing singlet oxygen yield of methylene blue (on the left hand graph) and rose bengal (on the right hand graph) both with sonic activation (square data points) and without sonic activation (diamond data points). The methylene blue graph (on the left hand side) shows that the initial absorbance of light is substantially higher when the micro-bubble solution is sonically activated. The absorbance of the light of the sonically activated micro-bubble solution then falls off much more rapidly than the absorbance of the solution without sonic activation. The rose bengal (RB) graph shows an absorbance level higher at all times for the test without sonic activation. However, as seen, in the sonically activated test, the absorbance fell off much more quickly than in the non-activated sample. The substantial fall off in absorbance in the sonically activated samples (as compared to the non-activated samples) for both the methylene blue and the rose bengal show substantial increase in micro-bubble activity within the first few seconds of activation of the micro-bubble solution. This thus demonstrates that the agitation enhances the singlet oxygen yield and subsequently the efficacy of photodynamic therapy.

[0096] Testing of Procedure

[0097] Often, testing is performed on immature (4-day old) biofilms. Immature, or young, biofilms are still soft, and can be easily removed. However, immature biofilms are rarely presented in an actual root canal procedure. In mature biofilms (i.e., 6 weeks old and older), the biofilm is calcified. The differences between the immature and mature biofilms can be seen in the photomicrographs of FIGS. 12 and 13.

[0098] FIG. 14 schematically demonstrates the strategy of my enhanced light activated disinfection method. The oxygen carrier ensures that there is a sufficient concentration of O.sub.2 in the root canal, because there usually is little to no O.sub.2 in a root canal. The oxidizer enhances the oxidation potential of the photosensitizing compound, and hence increases the number of oxygen singlets in the canal. In addition, the hydrogen peroxide in the micro-bubble solution (1) is activated by the low-energy level light and (2) will interact with organic debris within the root canal to form more free radicals which interact with gas within the micro-bubbles resulting in their growth and propulsion towards the root canal wall, as shown in FIGS. 24 and 25, in which the organic cell remnants are stained blue. Please note that the micro-bubbles expand to larger bubbles in proximity to organic remnants. This phenomenon allows the micro-bubbles to propel towards the organic materials. thereby facilitating debridement.

[0099] To test the effectiveness of the method, 50 single rooted teeth were initially sterilized and then then incubated with E. faecalis in AC Broth, an all culture broth available from Sigma-Aldrich, for four weeks. The teeth were divided into five groups (with 10 teeth in each group) as follows: [0100] Group 1: Control Group. In this group, the teeth received no disinfecting treatment. [0101] Group 2: Traditional RCT. In this group, the teeth were subjected to shaping and cleaning. The teeth were shaped using Protaper? Universal files (available from Dentsply Tulsa Dental), and flushed with 6 ml of 5.25% sodium hypochlorite solution for 180 seconds. [0102] Group 3: Conventional Light Activated Disinfection (LAD). In this group, the teeth were shaped with a Protaper? Universal file. The shaped canal was flushed with a 100 micro-molar solution of methylene blue in water for 300 seconds. The teeth were then subjected to light activation for 600 seconds without agitation. Light was introduced into the canal using the activator of FIG. 3, but without activating the activator. [0103] Group 4: SAMP. In this group, the teeth were disinfected via my photo-chemically activated micro-bubble based root canal disinfection in which the micro-bubbles are pulsated and propelled toward the root canal wall by the activation energy. Initially the root canals of the teeth were shaped with Protaper? Universal files. The shaped canal was then flushed for 300 seconds with a 100 micro-molar solution of methylene blue dissolved in polyethylene glycol, glycerol and water. The PEG, ethanol and water were combined in a 30:20:50 ratio by volume. The excess photosensitive solution was removed from the canal using paper points. The canal was then filled with a micro-bubble solution comprised of PFC, H.sub.2O.sub.2, and Triton X-100 in a 75.0:24.5:0.5 ratio by volume. The micro-bubble solution was sonically activated for 180 seconds using an EndoActivator? driver fitted with a fiber optic cable, and the fiber optic cable delivered light from a diode laser (660 nm) into the canal of the micro-bubble solution with the introduction of light into the canal. [0104] Group 5: RCT+SAMPIn this group, the teeth were shaped and cleaned in accordance with traditional RCT (as in Group 2) and were then subject to SAMP (as in Group 4).

[0105] After treatment, the teeth were cut axially to expose the canal of each tooth. Specimens of the root canal surface were taken and placed in Brain Heart Infusion (BHI) growth media to monitor the colony forming units. The samples were checked at four hours and again at twenty-four hours. In addition, the root canals were prepared from scanning electron microscopy.

[0106] FIG. 15 demonstrates the effectiveness of my disinfecting method. The first two columns of FIG. 15 contain images which show one week old bacterial biofilms of Enterococcus faecalis before and after treatment for the different groups. Each of the six images in the first two columns show active and dead bacteria, with the active bacteria being shown in green and the dead bacteria being shown in red. The two left-hand columns of FIG. 15 contains, images showing the three-dimensional confocal laser scanning microscopy reconstruction of the Enterococcus faecalis biofilms subjected to different treatments (60?). The left column shows the biofilm receiving no treatment (top), methylene blue (photosensitizer) (100 ?mol/L) treatment (middle) and PF4 micro-bubble solution treatment (bottom). The middle column shows the biofilm subjected to light (660 nm) irradiation alone (top), methylene blue+light (660 nm) irradiation (middle) and PF4 micro-bubble solution treatment+light (660 nm). Finally, right-hand column shows the sagittal section of the treatment groups as described in the middle column MB. As seen in the top row, a significant amount of active (live) bacteria exists after just cleaning and shaping. Disinfection using sodium hypochlorite, as shown in the second row, kills (eliminates) nearly all of the active bacteria. However, there active bacteria remain. As shown in the bottom row, my photoenhanced activated micro-bubble based disinfection method eliminates virtually all of the active bacteria.

[0107] Experiments were conducted on 4 weeks old biofilm of E. faecalis grown with root canals of single rooted teeth. The results of the five different groups of teeth are summarized in Table I below.

TABLE-US-00001 TABLE I EFFICACY OF BIOFILM DESTRUCTION No. of specimens positive CFU (log.sub.10) after after 24-hrs Treatment groups 4 hrs enrichment enrichment Group 1: Control 7.147 (?0.601) 100% Group 2: RCT 0.0 60% (Instrumentation with Protaper? F2 and sodium hypochlorite and EDTA irrigation) Group 3: Conventional-LAD 5.639 (?0.066) 100% (After instrumentation, methylene blue 80 micro molar solution was used to photosensitized and light activation (36J)) Group 4: SAMP (After 0.0 0% instrumentation, photosensitized with MB (5 min) and micro-bubbles were added and sonically activated with EndoActivator? driver for 5 min Group 5: RCT + SAMP 0.0 0% (combination of steps in groups 2 and 4)

[0108] The root samples were split into two halves. One half of the root was used for microbiological culture analysis (shown above) and described just below, and the other half was used for SEM analysis described below in conjunction with FIGS. 16-19. Eight tooth samples were used for microbiological culture analysis while four samples were used for SEM analysis under each group.

[0109] As seen, the control (Group 1) and conventional-LAD (Group 3) samples both had a significant number of colony forming units (CFU's) of active bacteria after four hours of enrichment in a growth media, and all of the samples of these two groups tested positive for colony forming units after 24 hours. In Group 2, where samples were treated via traditional root canal therapy (RCT), there were no colony forming units after four hours of enrichment in growth media. However, 60% of the Group 2 (RCT treated) samples had colony forming units after 24 hours in the growth/enrichment media. This is to be compared with the teeth of Groups 4 and 5 which were treated via SAMP and RCT+SAMP, respectively, as described above. In both on these groups, there were no colony forming units in the growth/enrichment media after four hours, and importantly, there were no colony forming units in the growth media after 24 hours. The 24 hour test demonstrates that the SAMP and RCT+SAMP treatment substantially destroyed all the bacteria in the canals. The teeth from Group 4 (SAMP alone) demonstrate that SAMP is highly effective, and the chemical disinfection used in traditional RCT need not be used if the canal is disinfected via my SAMP method.

[0110] FIGS. 16-19 contain scanning electron photomicrographs of the treated root canals of teeth from Groups 1, 3 (two variations) and 4. FIG. 16 contains photomicrographs of a tooth from Group 1 (control). As seen, the biofilm is still present. FIG. 17 contains photomicrographs of a tooth from Group 3 (PDT with MB in water without activation). As seen, there is some disruption of the biofilm, but the biofilm is still, in large measure, present on the root canal surface. FIG. 18 demonstrates the effect of the micro-bubble solution on conventional light activated disinfection (LAD). As seen, the biofilm is disrupted to a greater extent than in the photomicrographs of FIG. 17. However, the biofilm is still present. FIG. 18 contains photomicrographs of a tooth from Group 4 (SAMP). As seen, in the teeth treated via sonically activated micro-bubble based photodynamic therapy, the biofilm is fully removed, the canal wall can be clearly seen, and the dentinal tubules are opened.

[0111] FIGS. 20-22 contain color-enhanced images of a root canal comparing a control tooth with root canals treated via photoactivation of methylene blue in water in the presence of activated micro-bubble solution and photoactivation of methylene blue in water, ethanol and polyethylene glycol in the presence of activated micro-bubble solution, to demonstrate the effectiveness of the water/alcohol/PEG carrier solution. In these photographs, active bacteria are shown in green and dead bacteria as shown in red. FIG. 20 contains photomicrographs of a control tooth. These pictures show a significant amount of active bacteria (in green). The control biofilms show multilayered biofilm structure of more than 45 microns thickness adherent to the root dentin substrate FIG. 21 contains photomicrographs of a tooth treated with MB in a water carrier and then subject to sonic and light activation of the micro-bubble solution; and FIG. 22 contains photomicrographs of a tooth treated with MB in the polyethylene glycol, alcohol and water carrier solution, and then subject to sonic and light activation of the micro-bubble solution. Both FIGS. 21 and 22 show that substantially all of the active bacteria have been eliminated. These two figures also show that effect of the micro-bubble solution. Although use of the polyethylene glycol-alcohol-water carrier solution for the MB allows for better penetration of the MB into the dentin, the sonic and light activation of the micro-bubble solution compensates for this, and in both groups (FIGS. 21 and 22), the biofilm is substantially completely disrupted. Dead bacteria are noted even within the dentinal tubules.

[0112] The images in FIGS. 24 and 25 were obtained by interactive cell components in serum albumin mixed with micro-bubbles and observing the interaction under light microscopy. These images highlight the micro-bubble dynamics even without photoactivation.

[0113] As can be seen from the foregoing, dissolving of a photoactive compound in a water/alcohol solution enables the photosensitizing compound (i.e., the methylene blue) to better penetrate the dentinal tubules and anatomical complexities of the root canal. Further, the utilization of the micro-bubble solution produces a greater degree (more than 3?) of reactive oxygen (i.e., oxygen singlets), allows for better penetration of light into the dentin, improves the physical/mechanical effects of the micro-bubbles (pulsating/propelling), and thus increases the anti-biofilm efficacy of PDT. My enhanced micro-bubble based light activated disinfection disrupts and substantially eliminates the biofilm from the root canal system, and even in uninstrumented portions of the root canal. Thus, the disclosed method provides for a substantially better disinfection of the root canal than has been obtained with standard or conventional root canal treatment. This better disinfection of the canal allows for better results, and should result in fewer retreatments. Further, because uninstrumented portions of the canal are disinfected, SAMP allows for effective MIE.

[0114] As various changes could be made in the above constructions without departing from the scope of the invention, it is intended that all matter contained in the above description or shown in the accompanying drawings shall be interpreted as illustrative and not in a limiting sense.