Methods for non-surgical treatment of carpal tunnel syndrome

Abstract

Provided is a non-surgical method of treatment of carpal tunnel syndrome by administering an effective amount of purified collagenase I and II by injection onto a transverse carpal ligament, without penetrating the transverse carpal ligament. The purified collagenase I and II are obtained from Clostridium histolyticum, and the effective amount is a dose comprising a concentration of approximately 0.29 mg of the purified collagenase I and II to one ml diluent.

Claims

1. A non-surgical method of treating carpal tunnel syndrome in a subject in need thereof, the method comprising: administering by injection, an effective amount of purified collagenase onto a transverse carpal ligament of the subject, wherein the effective amount of the purified collagenase is at least 0.58 mg/ml with a specific activity of at least 1500 SRC units, wherein the purified collagenase is obtained from Clostridium histolyticum and comprises collagenase I and II, and wherein the collagenase is administered with a blunt needle.

2. The method of claim 1, wherein the collagenase is injected in a liquid pharmaceutically acceptable carrier.

3. The method of claim 1, wherein the effective amount of the purified collagenase is injected between three and six sites onto a palmar side of the transverse carpal ligament of the subject.

4. The method of claim 1, wherein the injection is onto the transverse carpal ligament, without penetrating the transverse carpal ligament.

5. The method of claim 1, wherein the injection is performed with ultrasound guidance to avoid penetration of the transverse carpal ligament.

6. The method of claim 1, wherein the purified collagenase functions to rupture the transverse carpal ligament.

7. The method of claim 1, wherein an anesthetic is injected prior to administration of the collagenase, and wherein the injection of the anesthetic makes a needle track.

8. The method of claim 7, wherein the collagenase is administered following the needle track.

9. The method of claim 2, wherein the concentration of the purified collagenase in the pharmaceutically acceptable liquid carrier is between 0.58 mg/ml and 1.16 mg/ml.

10. The method of claim 2, wherein the volume of the pharmaceutically acceptable liquid carrier is between 0.1 ml and 2.0 ml.

11. The method of claim 2, wherein the liquid pharmaceutically acceptable carrier is selected from the group consisting of normal saline, aqueous NaCl/CaCl.sub.2 buffer, aqueous dextran solution, and aqueous hetastarch solution.

Description

DETAILED DESCRIPTION OF EMBODIMENTS OF THE PRESENT INVENTION

(1) The present disclosure relates to the discovery that collagenase injections are effective to lyse collagenous adhesions in the carpal tunnel and treat carpal tunnel syndrome. As such, the disclosure relates to methods of treating or preventing carpal tunnel syndrome in a patient in need of such treatment includes injecting or otherwise delivering an effective amount of collagenase to the collagenous adhesions in the carpal tunnel. The disclosure also relates to the use of collagenase in the manufacture of a medicament to treat carpal tunnel. Collagenase injections have been proposed for the treatment of diseases such as Duptyren's disease and Peyronie's disease. Both diseases are associated with collagen plaques or cords. Wegman, Thomas L., U.S. Pat. No. 5,589,171, Dec. 31, 1996, U.S. Pat. No. 6,086,872, Jul. 11, 2000 and U.S. Pat. No. 6,022,539, Feb. 8, 2000. Collagenase is an enzyme that has the specific ability to digest collagen. A preferred form of collagenase is derived from fermentation by Clostridium histolyticum, and is purified by a chromatographic technique, as disclosed at U.S. Pub. No. 2007/0224183 A1 of Sabatino et al., issued as U.S. Pat. No. 7,811,560. Collagenase naturally produced by Clostridium histolyticum once purified will exhibit two distinct peaks when run on an electrophoresis SDS gel. These two distinct peaks are referred to as collagenase I and collagenase II.

(2) Sterilized lyophilized collagenase powder is commercially available having a minimum assay of 50 ABC units per mg. The assay may range considerably above that from batch to batch, but is taken into account in determining the weight of powder to use with a pharmaceutically acceptable carrier, e.g. normal saline, in preparing a desired concentration for treatment.

(3) A preferred collagenase composition includes a mixture of collagenase I and collagenase II in a mass ratio of about 1 to 1 and having specific activity of at least about 700 SRC units/mg, such as at least about 1,000 SRC units/mg, more preferably at least about 1500 SRC units/mg. One SRC unit will solubilize rat tail collagen into ninhydrin reaction material equivalent to 1 nanomole of leucine per minute, at 25 C., pH 7.4. Collagenase has been described in ABC units as well, with 10,000 ABC units equaling approximately 0.58 mg of collagenase. This potency assay of collagenase is based on the digestion of undenatured collagen (from bovine tendon) at a pH of 7.2 and 37 degrees C. for 20-24 hours. The number of peptide bonds cleaved is measured by reaction with ninhydrin. Amino groups released by a trypsin digestion control are subtracted. One net ABC unit of collagenase will solubilize ninhydrin reactive material equivalent to 1.09 nanomoles of leucine per minute. 1 SRC unit equal approximate 6.3 ABC units or 18.5 GPA units.

(4) The collagenase is administered via injection in a pharmaceutically acceptable liquid carrier that does not interact or deactivate the collagenase. Examples are normal saline, aqueous NaCl/CaCl.sub.2 buffer (e.g., containing 0.9% NaCl and 2 mM CaCl.sub.2), aqueous dextran solution, and aqueous hetastarch solution. For example, the lyophilized formulation can contain 0.1 mg lactose monohydrate per 1,000 ABC units, with each glass vial containing 5,150 ABC units of enzyme.

(5) In accordance with the present disclosure, collagenase in a liquid carrier is injected into a collagen adhesion within the carpal tunnel. The amount and concentration of collagenase used are effective to soften and relax or rupture the adhesion.

(6) AA4500, i.e., collagenase clostridium histolyticum, is injected onto, not into, the transverse carpal ligament using ultrasound guidance, in the proximal carpal tunnel (wrist crease), mid carpal tunnel (hook of the hamate) and distal carpal tunnel (distal edge of the ligament), in patients, to lyse type 1 collagen. The AA4500 functions as an enzymatic scalpel to rupture the transverse carpal ligament.

(7) The tunnel contains nine tendons and the median nerve. The thickness of the transverse carpal ligament is ascertained in the proximal, mid and distal carpal tunnel for each patient.

(8) Using ultrasound guidance, an 18 gauge needle punctures the skin, without puncturing the transverse carpal ligament, in the proximal, mid and distal carpal tunnel, simulating a lidocaine injection, to provide needle tracks for subsequent AA4500 delivery.

(9) Using ultrasound guidance, a blunt tipped needle (Sprotte) follows the needle tracks. The AA4500 is injected onto, without penetrating, the transverse carpal ligament of each hand of a patient, in the proximal, mid and distal tunnel in three equal aliquots of 0.29 mg AA4500/1 ml, with a concentration of 0.29 mg/ml. Alternative embodiments are 0.58 mg AA4500/1 ml, with a concentration of 0.58 mg/ml, 0.58 mg AA4500/2 ml, with a concentration of 0.29 mg/ml, and 0.58 mg AA4500/0.5 ml, with a concentration of 1.16 mg/ml.

(10) The AA4500 is injected with 1 ml Endo dilution, with a dosing group (n=2) and a placebo group (n=4), with a placebo of 1 ml, for control since the placebo will not rupture the transverse carpal ligament.

(11) Twenty-four hours are allowed to lapse following the injection, for collagen lysis of the transverse carpal ligament. After lapse of the twenty-four hours, each hand of the patient is placed, palmar surface down, on a table top. A gentle pressure applied to force the hand flat against the table top, and manipulation procedure is performed utilizing a specially designed instrument having a steel ball attached to a standard tip/pinch meter, to apply a force of pressure measured in kg or lbs. The transverse carpal ligament is aligned on the steel ball and pressure is applied from the dorsal side of the hand to at least 10 lbs.

(12) Ultrasound imaging is performed at the proximal, mid and distal carpal tunnel to detect thickness of the transverse carpal tunnel or evidence that the ligament has been partially or completely ruptured by the AA4500.

(13) The total volume of liquid injected is preferably 2.0 ml or less, with a smaller volume down to about 0.5 ml to about 0.1 ml being preferred.

(14) The injection is preferably administered with an anesthesia injection of 5-10 ml sterile 1% lidocaine preferably preceding the collagenase injection for patient comfort.

(15) The total dosage is preferably injected in one portion, although two or more portions at the same or different but adjacent points are possible, to assure good distribution of the collagenase within a small volume of the adhesion.

(16) The patient can be any animal, preferably a mammal or human patient. Examples of animals that can be treated according to the present disclosure include domestic animals (such as cats, dogs, etc.), farm animals (such as horses, cows, pigs, etc.) and exotic animals (such as monkeys, apes, etc.). Preferred human patients are those that have decreased finger/carpal tunnel motion which occurred idiopathically, after trauma or patients with diabetes and/or thyroid disorders.

(17) The patient is characterized as having pain that has increased to a level that interferes with activities of daily living and diminishes the quality of life. The present disclosure achieves improvement in all planes of motion.

(18) Accordingly, a non-surgical method is provided for treatment of carpal tunnel syndrome that includes administering an effective amount of purified collagenase I and II by injection onto a transverse carpal ligament of the patient, with the injection not penetrating the transverse carpal ligament, with the purified collagenase I and II being obtained from Clostridium histolyticum, and with the effective amount being a dose comprising a concentration of 0.29 mg or more of the purified collagenase I and II to one ml diluent.

(19) While the invention has been shown and described with reference to certain embodiments thereof, it will be understood by those skilled in the art that various changes in form and detail may be made therein without departing from the spirit and scope of the invention as defined by the appended claims, and equivalents thereof.