Automatic analyzer
10067152 ยท 2018-09-04
Assignee
Inventors
- Masaru MIYAZAKI (Tokyo, JP)
- Takamichi MORI (Tokyo, JP)
- Kazuhiro Nakamura (Tokyo, JP)
- Isao Yamazaki (Tokyo, JP)
Cpc classification
G01N21/75
PHYSICS
International classification
G01N35/10
PHYSICS
Abstract
An automatic analyzer has a washing tank providing water for washing a reagent or sample probe is. The washing water from a washing nozzle spreads from a throttle portion and is divided into right and left flows after colliding with a vent plate provided in an overflow portion of the washing tank. The washing water flows between the vent plate and the inner wall of the overflow portion, and the flows join behind the vent plate. After joining, the washing water flows downward along the vent plate and the inner wall of the overflow portion and then is drained. Because a space is created between the washing water which has collided with the vent plate and the washing water joined behind the vent plate, the washing water is prevented from completely covering the overflow portion, and the airflow can be secured during the drainage.
Claims
1. An automatic analyzer comprising: a probe that suctions a reagent or a sample and discharges the reagent or the sample into a reaction container, a dispensing mechanism that moves the probe vertically and horizontally, a suction and discharge mechanism that causes the probe to suction the reagent or the sample and discharge the reagent or the sample into the reaction container, a photometer that analyzes the sample in the reaction container, a washing tank that has a first opening into which the probe is inserted and that washes the probe inserted through the opening, and a controller that controls operations of the dispensing mechanism, the discharge mechanism, the photometer and the washing tank, wherein the washing tank comprises: a washing portion for washing the probe inserted through the first opening, a first washing nozzle that discharges washing water to the first opening, a suction nozzle that suctions air from the washing portion, an overflow portion that has a second opening through which the washing water discharged from the first washing nozzle to the first opening drains downward, and a vent plate attached to the overflow portion and that has a smaller width than an opening size of the second opening of the overflow portion, and that is disposed to extend within the overflow portion through the second opening from an upper part to a lower part of the overflow portion.
2. The automatic analyzer according to claim 1, wherein: the washing tank further comprises a throttle portion which has a smaller diameter than an opening size of the first opening and which is provided between the first opening and the washing portion, wherein the probe is inserted into the throttle portion, the first washing nozzle supplies the washing water to the first opening from the side, and the washing water discharged from the first washing nozzle collides with the vent plate and is introduced to the overflow portion by the vent member.
3. The automatic analyzer according to claim 1, wherein the vent plate has an approximately C-shaped cross-section.
4. The automatic analyzer according to claim 1, wherein the vent plate has an approximately V-shaped cross-section.
5. The automatic analyzer according to claim 3, wherein the vent plate is integrated into the overflow portion.
6. The automatic analyzer according to claim 4, wherein the vent plate is integrated into the overflow portion.
7. The automatic analyzer according to claim 1, wherein the vent plate is an elastic member.
8. The automatic analyzer according to claim 1, wherein an end of the vent plate is rotatably supported on the overflow portion.
9. An automatic analyzer comprising: a probe that suctions a reagent or a sample and discharges the reagent or the sample into a reaction container, a dispensing mechanism that moves the probe vertically and horizontally, a suction and discharge mechanism that causes the probe to suction the reagent or the sample and discharge the reagent or the sample into the reaction container, a photometer that analyzes the sample in the reaction container, a washing tank that has a first opening into which the probe is inserted and that washes the probe inserted through the opening, and a controller that controls operations of the dispensing mechanism, the discharge mechanism, the photometer and the washing tank, wherein the washing tank comprises: a washing portion for washing the probe inserted through the first opening, a first washing nozzle that discharges washing water to the first opening, a suction nozzle that suctions air from the washing portion, an overflow portion that has a second opening through which the washing water discharged from the first washing nozzle to the first opening drains downward, and a cylindrical vent member attached to the overflow portion and that has a smaller width than an opening size of the second opening of the overflow portion, and that is disposed to extend within the overflow portion through the second opening from an upper part to a lower part of the overflow portion, wherein the vent member has perforations in a side face thereof.
Description
BRIEF DESCRIPTION OF DRAWINGS
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DESCRIPTION OF EMBODIMENTS
(20) Embodiments of the invention are explained below referring to the attached drawings.
EXAMPLES
Example 1
(21)
(22) In
(23) Reagent-dispensing mechanisms 7 and 8, which can rotate and move vertically, are provided between the reaction disk land the reagent disk 9, and the reagent-dispensing mechanisms 7 and 8 each have a reagent probe 7a. A reagent syringe 18 is connected to the reagent probes 7a.
(24) A sample-dispensing mechanism 11, which can rotate and move vertically, is provided between the reaction disk 1 and the sample transportation mechanism 17, and the sample-dispensing mechanism 11 has a sample probe 11a.
(25) A sample syringe 19 is connected to the sample probe 11a. The sample probe 11a moves, drawing an arc with a rotation axis for its center, and dispenses the sample in a sample container 15 to a reaction cell (reaction container) 2.
(26) A washing mechanism 3, a spectrophotometer 4, stirring mechanisms 5 and 6, the reagent disk 9 and the sample transportation mechanism 17 are provided around the reaction disk 1, and a washing pump 20 is connected to the washing mechanism 3. A washing tank 13 for the sample probe 11a of the sample-dispensing mechanism 11, 30 and 31 for the stirring mechanisms 5 and 6 and washing tanks 32 and 33 for the reagent probes 7a are provided on the working ranges of the reagent-dispensing mechanisms 7 and 8, the sample-dispensing mechanism 11 and the stirring mechanisms 5 and 6, respectively. The sample containers 15 contain test samples such as blood, and the sample containers 15 on the rack 16 are transported by the sample transportation mechanism 17. The mechanisms are connected to a controller 21, and the operations are controlled by the controller 21.
(27) The sample to be tested in a sample container 15 is dispensed to a reaction cell 2 by the sample probe 11a, and the reagent in a reagent bottle 10 is also dispensed to the reaction cell 2 by the reagent probe 7a. The sample and the reagent are stirred in the reaction cell 2 by the stirring mechanisms 5 and 6. Light emitted from a light source is applied to the mixture solution of the sample and the reagent, and the spectrophotometer 4 receives the light applied. The controller 21 calculates the concentration of a certain component contained in the sample from the intensity of the light received. By such a method, the sample is analyzed.
(28) The above configuration is a general configuration of an automatic analyzer.
(29) Next, referring to
(30) The oblique sectional view of the washing tank shown in
(31) The structure having the shape shown in
(32) In
(33) A washing portion 205 for washing the reagent probe 7a is provided under the opening 311. A washing nozzle 202 is connected to the washing portion 205.
(34) The washing water is supplied from the washing nozzle 202 to the washing portion 205 in the washing tank, and the washing water is applied to the reagent probe 7a to wash the reagent probe 7a. During this operation, the electromagnetic valve 302 is open. After the reagent probe 7a is washed, the electromagnetic valve 302 under the waste liquid portion 221 is closed, and vacuum nozzles 212a, 212b and 212c connected to the washing portion 205 in the washing tank and a vacuum nozzle 211 connected to the opening 311 start vacuum-suctioning. The reagent probe 7a is raised during the vacuum-suction.
(35) Here, because the vacuum nozzles 212a, 212b and 212c suction air and thus remove the washing water on the reagent probe 7a, the opening of the throttle portion 301 is desirably as narrow as possible from the viewpoint of the improvement of the drying property. When the opening is narrow, however, it is supposed that the reagent scatters around the throttle portion 301 while the reagent probe 7a is lowered in the washing tank, and it is thus necessary to wash the upper surface of the throttle portion 301 with the washing water. Therefore, the flow path of the washing water between the washing nozzle 201 and an overflow portion 222 is configured to be narrow with the flow path throttle portion 305 so that a certain amount of the washing water stays on the throttle portion 301, and this configuration allows washing of the throttle portion 301. In this regard, for convenience, the number of the vacuum nozzles is four (211, 212a, 212b and 212c), but the number of the vacuum nozzles does not have influence because the balance with the vacuum force is involved. Also, as another example, separate flow paths may be provided for the washing water from the washing nozzle 201 and that from the washing nozzle 202, and the pressures of the washing water may be different from each other.
(36) When the electromagnetic valve 302 is closed, air enters the washing tank only from the throttle portion 301. Since the inside diameter of the throttle portion 301 is small, the air which has entered from the throttle portion 301 blows off the washing water remaining on the reagent probe 7a and removes the washing water.
(37) The washing water is supplied to the washing nozzles 201 and 202 from the washing pump 20.
(38) As shown in
(39) The operations of the electromagnetic valve 240, the vacuum tank 241 and the vacuum pump 242 are controlled by the controller 21.
(40) The vacuum nozzle 212c is not shown in
(41)
(42) In addition, it is necessary to increase the pressure of the washing water and wash the reagent probe 7a with a higher amount of water in order to wash the reagent probe 7a sufficiently in a short time. As a result, the washing water floods into the overflow portion 222. The washing water cannot be drained through the overflow portion 222, and the washing water used for washing the probe 7a may overflow in the washing tank as shown in
(43) As shown in
(44) As shown in
(45) Here, assuming that the downward pressure exerted on a liquid surface is represented by gh ( is the density, g is the gravitational acceleration, and h is the height), the pressure causing the washing water to drain under its own weight decreases as the density reduces. On the other hand, the buoyancy force is exerted on the air bubbles surrounded by the washing water from top and bottom. When the buoyancy force and the force causing the washing water to drain under its own weight balance, the washing water cannot be drained.
(46) For example, when the diameter of the overflow portion 222 is 6 mm, the ratio of the air bubbles and the washing water is 1:1 and the height of the liquid surface is 1 m; the apparent density of the washing water is and the pressure at the liquid surface is about 0.14 N when the friction in the tube and the like are ignored. When the air bubbles account for half of the volume of the tube, the buoyancy force is calculated also at about 0.14 N, which is almost balanced with the pressure, and the drainage is not possible.
(47) Furthermore, when the flow velocity of the washing water is high, the flow of the washing water is split. When the upper flow of the split washing water collides with the inner wall of the washing tank, the washing water swirls in the upper part of the overflow portion 222 and joins the washing water discharged from the washing nozzle 201. As a result, the washing water gathers in the upper part of the overflow portion 222. At this point, since the force by which the washing water drains under its own weight has been reduced, the washing water gathers in the washing tank. When the height of the washing water exceeds the height of the washing water discharged from the washing nozzle 201, the washing water flows backward into the throttle portion 301.
(48) When the washing water used for washing the nozzle overflows in the washing tank, flows backward and adheres to the reagent nozzle 7a, the washing water remains on the reagent nozzle 7a in a part higher than the range to be dried by vacuum-suction. This greatly affects the reliability of the dispensing operation, for example because the reagent is diluted with the washing water brought into the reagent bottle with the reagent probe 7a when the next reagent is suctioned or because the washing water drips down into a neighboring reaction container when the reagent is discharged.
(49) Also, when the washing water used for washing the reagent probe adheres to the reagent probe, the washing water used for washing adheres to the reagent nozzle 7a again. When the reagent probe 7a in this state is inserted into the reagent bottle, the reagent is contaminated or diluted. Moreover, when the washing water overflows the washing tank and spills out in the apparatus, the connectors or the wires may be short-circuited. In order to avoid the risks of the deterioration of the dispensing reliability or apparatus troubles, it is essential to drain the washing water without overflow.
(50) When the washing water flows at a high pressure, the flow velocity of the washing water is high, and the flow is a turbulent flow. After the washing water is discharged from the washing nozzle 201, there are factors which affect the flow such as the reagent nozzle 7a, the difference in level, the throttle around the center and the slope, until the washing water is drained into the overflow portion 222. Depending on the flow of the washing water which flows towards the overflow portion 222, the washing water does not completely cover the overflow portion 222, and the washing water is drained without overflow because a space is created. Thus, the washing water sometimes overflows in the washing tank and sometimes does not.
(51) When the ratio of the amount of the washing water drained and the air bubbles is 6:4, the permissible flow rate of the drainage through the overflow portion 222 with a diameter of 6 mm is about 20.5 mL/s according to the Torricelli's equation.
(52) On the other hand, when the necessary flow rate for washing the reagent probe 7a is about 21 mL/s, the flow rate of the washing liquid flowing into the overflow portion 222 exceeds the permissible flow rate of the overflow portion 222.
(53) Because the flow rate of the washing water cannot be decreased to maintain the capability of washing the reagent probe 7a, the washing water cannot be drained unless the diameter of the overflow portion 222 is increased.
(54) In order to leave a margin of about two times for the permissible flow rate of the overflow portion 222 relative to the flow rate of the washing water, it is necessary that the diameter of the overflow portion 222 is 1.5 times larger or more. However, to actually install the washing tank in the apparatus, the reduction of the size of the washing tank is essential. Thus, the diameter of the overflow portion 222 cannot be increased.
(55) In order to increase the permissible flow rate while maintaining the current diameter of the overflow portion 222, it is essential to remove the air bubbles. When the proportion of the air bubbles is reduced to 10% or less, the permissible flow rate of the overflow portion 222 becomes 31 mL/s or more, and a margin of 1.5 times or more can be left relative to the flow rate of the washing water.
(56) Therefore, in order to drain the washing water efficiently, it is essential to prevent the air bubbles of the sizes which block the overflow portion 222 from being generated in the overflow portion 222.
(57) Next, Example 1 of the invention which is capable of removing the air bubbles from the overflow portion 222 and draining the washing water efficiently is explained referring to
(58) In Example 1 shown in
(59) The washing water discharged from the washing nozzle 201 spreads from the throttle portion 305 at the center and flows into the overflow portion 222. The washing water collides with the vent plate 303 and is divided into right and left flows, in 180-degree opposite directions, at the vent plate 303. The washing water flows between the vent plate 303 and the inner wall of the overflow portion 222, and the flows join behind the vent plate 303.
(60) After joining behind the vent plate 303, the washing water flows downward along the vent plate 303 or the inner wall of the overflow portion 222 and then is drained. At this point, because a space is created between the washing water which has collided with the vent plate 303 and the washing water joined behind the vent plate 303, the washing water can be prevented from completely covering the flow path in the overflow portion 222. Also, since there is a space between the flows of the washing water divided by the vent plate 303, the airflow can be secured during the drainage.
(61) As a result, the air in the overflow portion 222 is released upward (indicated with the dashed arrow in
(62) The horizontal section of the vent plate 303 has a shape which can secure the airflow between the vent plate 303 and the washing water joined behind the vent plate 303. For example, when the vent plate 303 is a solid cylinder, no space is created between the vent plate 303 and the washing water because the overflow portion 222 has a cylindrical shape.
(63) When a vent plate 303 having a width of 5 mm is placed in the middle of an overflow portion 222 having a diameter of 8 mm under the conditions of
(64) When the width of the vent plate is reduced to about 1 mm, however, it is thought that the space between the washing water and the vent plate disappears when the right and left flows of the washing water, which have been divided at the vent plate 303, join. On the contrary, when the width of the vent plate 303 is almost the same as the diameter of the overflow portion 222, the path is divided by the vent plate 303 into a flow path in which the air flows upward and a flow path for draining the washing water, and the permissible flow rate is reduced by half. At this point, when the flow rate of the washing water flowing into the overflow portion 222 exceeds the permissible flow rate of the overflow portion 222, the washing water overflows. Thus, the width of the vent plate 303 is desirably about 50 to 70% of the diameter of the overflow portion 222.
(65)
(66) It is necessary that the height of the vent plate 303 is above the height of the flow of the washing water, as shown in
(67) Regarding the length of the vent plate 303, it is necessary that the vent plate 303 extends with enough length to a position below the air bubbles shown in
(68) The vent plate 303 is attached at a position a little closer to the throttle portion 301 from the center of the overflow portion 222. For example, when the diameter of the overflow portion 222 is 8 mm and the width of the vent plate 303 is 5 mm, the vent plate 303 is attached at a position about 1 to 2 mm closer to the throttle portion 301 from the center of the overflow portion 222.
(69) This is because a space is created easily between the vent plate 303 and the washing water which has been divided and then joined. When the vent plate 303 is 2 mm or more closer to the throttle portion 301, the level of the washing water which has collided with the vent plate 303 may rise remarkably along the vent plate 303. On the other hand, when the vent plate 303 is placed further from the throttle portion 301, the space may not be created between the vent plate 303 and the washing water.
(70) Accordingly, the position of the vent plate 303 is about 1 to 2 mm closer to the throttle portion 301 from the center of the overflow portion 222.
(71) As described above, according to Example 1 of the invention, it is possible to remove the air bubbles from the overflow portion 222 and drain the washing water efficiently, because the plate-like vent plate 303 with appropriate length and width is provided in an upstream part of the overflow portion 222 and the washing water used for washing the reagent probe 7a is divided and then caused to flow into the overflow portion 222. Also, even when the washing water is discharged from the washing nozzle 201 at a flow rate and a flow velocity which completely cover the overflow portion 222 to trap the air and cause the above problems when the vent plate 303 is not provided, the provision of the vent plate 303 allows washing of the reagent probe 7a without decreasing the flow rate and the flow velocity and allows efficient drainage of the washing water.
(72) Accordingly, an automatic analyzer having a washing tank from which the water for washing the reagent probe can be drained efficiently and which is capable of further increasing the flow velocity and the flow rate of the water for washing the reagent probe and improving the washing effect can be developed.
Example 2
(73)
(74) In Example 2 shown in
(75) Advantages of the example shown in
(76) The same effects as those of Example 1 can be obtained also by Example 2 of the invention. Furthermore, Example 2 also has effects of dividing the washing water smoothly and securing the airflow more easily.
Example 3
(77)
(78) Example 3 shown in
(79) The same effects as those of Example 1 can be obtained also by Example 3 of the invention. Furthermore, Example 3 also has effects of dividing the washing water smoothly and securing the airflow more easily.
Example 4
(80)
(81) Example 4 shown in
(82) The shape of the example shown in
(83) An example of the attachment in
(84) The same effects as those of Example 1 can be obtained also by Example 4 of the invention. Furthermore, Example 4 also has effects of dividing the washing water smoothly and securing the airflow more easily.
Example 5
(85)
(86) Example 5 shown in
(87) The cylinder is formed through the vent plate 303 from the upper end to the lower end. Also, by using a hydrophobic material for the vent plate 303 and forming minute holes in the wall of the cylinder, a liquid cannot enter from the holes, and only air can enter the cylinder and can be emitted. While the air can be emitted only from the upper end of the cylinder when the wall of the cylinder does not have the holes, the air can be emitted through the holes in the wall of the cylinder, regardless of the heights of the air bubbles, when holes are formed in the wall in the height direction.
(88) The other components are the same as those of the example shown in
(89) The same effects as those of Example 1 can be obtained also by Example 5 of the invention. Furthermore, Example 5 also has effects of emitting the air from the holes in the wall of the cylinder and securing the airflow more easily.
Example 6
(90)
(91) Example 6 shown in
(92) By integrating the vent plate 303 into the washing tank, the space for the ventilation can be secured certainly. Thus, the washing water can be drained smoothly even when the flow rate is high. Also, when the vent plate 303 is integrated into the washing tank, the number of the parts can be reduced.
(93) Furthermore, by forming holes in the wall of the vent plate 303 as in the example shown in
(94) The height of the vent plate 303 in
(95) The same effects as those of Example 1 can be obtained also by Example 6 of the invention. Furthermore, Example 6 has not only an effect of draining the washing water smoothly even when the flow rate is high but also an effect of reducing the number of the parts since the vent plate 303 is integrated into the washing tank.
Example 7
(96)
(97) Example 7 shown in
(98) In the example shown in
(99) The same effects as those of Example 1 can be obtained also by Example 7 of the invention. Furthermore, Example 7 has not only an effect of draining the washing water smoothly even when the flow rate is high but also an effect of reducing the number of the parts since the vent plate 303 is integrated into the washing tank.
Example 8
(100)
(101) Although the vent plate 303 is provided vertically in the examples in
(102) Example 8 of the invention is an example taking the above issue into consideration, and the material of the vent plate 303 is elastic (elastic member) in this example.
(103) In
(104) As the flow velocity of the washing water from the washing nozzle 201 becomes high, the degree of the bend of the vent plate 303 becomes large, and the flow of the washing water can be caused to be further downward. The position and the height of the vent plate 303 in
(105) The same effects as those of Example 1 can be obtained also by Example 8 of the invention. Furthermore, Example 8 also has an effect of preventing the washing water from scattering upward by causing the washing water to flow downward after colliding with the vent plate 303 and to drain easily and an effect of draining the washing water smoothly even when the flow rate is high.
Example 9
(106)
(107) Example 9 shown in
(108) The position and the height of the vent plate 303 in
(109) The same effects as those of Example 1 can be obtained also by Example 9 of the invention. Furthermore, Example 9 also has an effect of preventing the washing water from scattering upward by causing the washing water to flow downward after colliding with the vent plate 303 and to drain easily and an effect of draining the washing water smoothly even when the flow rate is high.
(110) Although the washing tank according to the invention has been described with respect to the reagent probe, in another dispensing style, the sample probe is thrust deep into the specimen in the sample container 15 to suction the sample from the bottom of the sample container 15. The washing range of the probe can be applied not only to the reagent probe but also to the sample probe, and the invention can be applied to a wide range of washing tanks.
(111) Accordingly, the washing tank of the invention is not limited to one for a reagent probe only, but the washing tank is effective for conducting the operations of from washing to drying at the same position and washing a probe which should be washed over a wide range.
(112) In addition, the invention can be achieved not only as an automatic analyzer having the above washing tank but also as the washing tank alone. This is because the washing tank alone can be manufactured and sold.
(113) In the present description, representative examples of the washing tank of the invention have been explained using
REFERENCE SIGNS LIST
(114) 1 . . . Reaction disk, 2 . . . reaction container, 3 . . . washing mechanism, 4 . . . spectrophotometer, 5 and 6 . . . stirring mechanisms, 7 and 8 . . . reagent-dispensing mechanisms, 7a . . . reagent probe, 9 . . . reagent disk, 10 . . . reagent bottle, 10a . . . detergent bottle, 11 . . . sample-dispensing mechanism, 11a . . . sample probe, 13 . . . washing tank for sample-dispensing mechanism, 15 . . . sample container, 16 . . . rack, 17 . . . sample transportation mechanism, 18 . . . reagent syringe, 19 . . . sample syringe, 20 . . . washing pump, 21 . . . controller, 30 and 31 . . . washing tanks for stirring mechanisms, 32 and 33 . . . washing tanks for reagent-dispensing mechanisms, 201 and 202 . . . washing nozzles, 211, 212a, 212b and 212c . . . suction nozzles, 221 . . . waste liquid portion, 222 . . . overflow portion, 301 . . . throttle portion, 302 . . . electromagnetic valve, 303 . . . vent plate, 304 . . . fixation pin, 305 . . . flow path throttle portion, 306 . . . groove, and 311 . . . opening.