Toxin binding system

Abstract

In some embodiments, a composition and/or method may include a toxin binding system formulated for safe and effective mixture into various feed rations which are fed to monogastric and ruminant animals such as poultry, swine, cows, cattle, and fish, among others. The toxin binding system includes novel combinations of one or more of an organoclay, an activated hydrated sodium calcium aluminosilicate clay, and a synthetic hectorite clay. In some embodiments, the binding composition may include organoclay, bentonite, hectorite, Leonardite, and/or any combination thereof. The toxin binding complex may effectively bind mycotoxins, endotoxins and some pesticides in the animal's digestive system, preventing their absorption and the consequent damages to the animal. This binding action includes the T-2 toxin, which can start their damaging action in the animal's mouth, hence, offering protection from oral lesions.

Claims

1. A method of inhibiting and/or ameliorating a malady associated with a mycotoxin, a pesticide, and an endotoxin, comprising: combining a composition with an animal feed, wherein the composition comprises: an organoclay; bentonite; synthetic hectorite; and Leonardite humic acid; providing the animal feed to an animal whereby the composition binds to at least one mycotoxin within the animal, at least one pesticide within the animal, and at least one endotoxin within the animal, wherein the composition is configured to bind to the at least one mycotoxin, the at least one pesticide, and the at least one endotoxin when the composition is exposed to the at least one mycotoxin, the at least one pesticide, and the at least one endotoxin associated with the animal, wherein the at least one mycotoxin comprises Aflatoxin, Ochratoxin, Zearalenone and Fumonisin, and wherein the at least one pesticide comprises Dieldrin, Diazinon and Malathion; wherein the animal is a farmed animal.

2. The method of claim 1, wherein the farmed animal comprises ruminant animals.

3. The method of claim 1, wherein the at least one mycotoxin, the at least one pesticide, and the at least one endotoxin is biologically innocuous to the animal by the binding of the at least one mycotoxin, the at least one pesticide, and the at least one endotoxin to the composition.

4. The method of claim 1, wherein the bentonite comprises between about 50% and about 75% of the binding composition.

5. The method of claim 1, wherein the organoclay comprises between about 20% and about 40% of the binding composition.

6. The method of claim 1, wherein the synthetic hectorite comprises between about 0.5% and about 5.0% of the binding composition.

7. The method of claim 1, wherein the hectorite comprises between about 0.5% and about 5.0% of the binding composition.

8. The method of claim 1, wherein the composition is configured to bind to, on average, at least 86% of the at least one mycotoxin.

9. The method of claim 1, wherein the composition is configured to bind to, on average, at least 99% of the at least one endotoxin.

10. The method of claim 1, wherein the composition is configured to bind to, on average, at least 70% of the at least one pesticide.

11. The method of claim 1, wherein the composition is configured to bind to, on average, at least 86% of the at least one mycotoxin, wherein the composition is configured to bind to, on average, at least 99% of the at least one endotoxin, and wherein the composition is configured to bind to, on average, at least 70% of the at least one pesticide.

12. The method of claim 1, wherein the farmed animal comprises poultry, swine, cattle, sheep, goats, horses, and fish.

Description

DETAILED DESCRIPTION

Definitions

(1) Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art.

(2) The term bentonite as used herein generally refers to an absorbent aluminium phyllosilicate clay consisting mostly of montmorillonite.

(3) The term connected as used herein generally refers to pieces which may be joined or linked together.

(4) The term coupled as used herein generally refers to pieces which may be used operatively with each other, or joined or linked together, with or without one or more intervening members.

(5) The term directly as used herein generally refers to one structure in physical contact with another structure, or, when used in reference to a procedure, means that one process effects another process or structure without the involvement of an intermediate step or component.

(6) The term synthetic hectorite as used herein generally refers to a rare soft, greasy, white clay mineral with a chemical formula of Na.sub.0.3(Mg,Li).sub.3Si.sub.4O.sub.10(OH).sub.2.

(7) The term humic acid as used herein generally refers to a principal component of humic substances, which are the major organic constituents of soil (humus), peat and coal. It is also a major organic constituent of many upland streams, dystrophic lakes, and ocean water. It is produced by biodegradation of dead organic matter. It is not a single acid; rather, it is a complex mixture of many different acids containing carboxyl and phenolate groups so that the mixture behaves functionally as a dibasic acid or occasionally, as a tribasic acid.

(8) The term monogastric as used herein generally refers to an animal having a simple single-chambered stomach.

(9) The term mycotoxin as used herein generally refers to a toxic secondary metabolite produced by organisms of the fungi kingdom, commonly known as molds.

(10) The term organoclay as used herein generally refers to an organically modified clay (e.g., an organically modified phyllosilicate), derived from a naturally occurring clay mineral. By exchanging the original interlayer cations for organocations (typically quaternary alkylammonium ions) an organophilic surface is generated, consisting of covalently linked organic moieties.

(11) The term ruminant as used herein generally refers to mammals that are able to acquire nutrients from plant-based food by fermenting it in a specialized stomach compartment (rumen) prior to digestion, principally through microbial actions.

(12) As previously indicated, the compositions are directed to a mycotoxins binding composition. More in particular, the present mycotoxins binding composition may be formulated for mixture into animal feed which are used as a food source for farming/raising of monogastric and ruminal animals including, but in no manner limited to, poultry, swine, dairy and beef cattle, sheep, goats, horses, and fish. As previously stated, mycotoxin contamination can occur in crops growing in the field, or it can be introduced during harvest, storage and/or processing of the animal feed. Pesticides could also be found in feedstuffs. Endotoxins can contaminate feedstuffs, if they are mix with animal by-products (e.g., blood meal contaminated with endotoxins).

(13) In some embodiments, a toxin binding system may include an organoclay. Organoclay may include an organically modified phyllosilicate, derived from a naturally occurring clay mineral. By exchanging the original interlayer cations for organocations (typically quaternary ammonium/surfactants) an organophilic surface may be generated, capable of binding a wider range of toxins than the original clay, including but not limited to, mycotoxins, such as derive from tricothecenes fungi, endotoxins, and pesticides.

(14) In another embodiment of the present invention, a toxin binding system may include an aluminosilicate clay. In at least one embodiment, the aluminosilicate clay may include a sodium calcium aluminosilicate clay. In some embodiments, a toxin binding system may include a hydrated sodium calcium aluminosilicate clay, and in one embodiment, an activated hydrated sodium calcium aluminosilicate clay. Bentonite may be included as an example of an aluminosilicate clay.

(15) As will be appreciated by those of skill in the art, both organoclay and activated hydrated sodium calcium aluminosilicate clay are lipophilic, and will bind fats, oils, and other lipids. As discussed above, organoclays and sodium calcium aluminosilicate clays have been utilized as an additive in animal feeds and have been found to be effective in binding with mycotoxins in the gastrointestinal tract of animals including but not limited to poultry, swine, cows, cattle, and fish. Each of these clays has been found to be effective in binding endotoxins and certain pesticides which could found their way into the gastrointestinal tract of animals. In some embodiments, neither organoclay nor activated hydrated sodium calcium aluminosilicate clay bind beneficial constituents inherent within or added to animal feeds, such as, amino acids, vitamins, minerals, antibiotics, pigments, coccidiostats, etc.

(16) As noted above, both the organoclay and the activated hydrated sodium calcium aluminosilicate clay are lipophilic, and as such, neither of these clays are effective in binding to hydrophilic mycotoxins. As such, in at least one embodiment, a toxin binding system may include an amount of a hydrophilic clay. In one embodiment, the present system may include an amount of a synthetic hectorite clay, such as is described in detail in U.S. Pat. No. 3,586,478, which is incorporated herein by reference in its entirety. A synthetic hectorite clay may be readily dispersible in water or other aqueous solvents. In some embodiments, the composition may include naturally occurring hectorite.

(17) As such, a toxin binding system may include a combination of both a lipophilic clay, namely, organoclay and/or sodium calcium aluminosilicate, and a hydrophilic clay (e.g., a synthetic hectorite clay). Therefore, the toxin binding system may be effective in binding mycotoxins present in the animal's gastrointestinal tract, such as via contaminated animal feed. A toxin binding system may be effective in binding water soluble mycotoxins, such as, by way of example only, T-2 toxin which must be bound in the animal's mouth and the gastrointestinal tract. An effective T-2 toxin adsorbent may diminish or prevent the adsorption of T-2 toxin at the intestinal level and reduce the oral lesions caused by T-2 toxin excreted through the saliva. In some embodiments, the toxin binding system may be effective in binding endotoxins and pesticides also present in the gastrointestinal track of the animal via contaminated feedstuffs and via bacterial growth.

(18) In some embodiments, a toxin binding system may include one or more humic acids. In some embodiments, humic acids may be hydrophilic. Humic acids may bind one or more mycotoxins. The humic acid may form between about 0.5% and about 5.0% of the binding composition. In some embodiments, humic acid may be provided by Leonardite. The term humic acid is a generic name. Leonardite comes only from the states of Wyoming, North and South Dakota.

(19) In some embodiments, a toxin binding system may include one or more types of bentonite. In some embodiments, bentonite may bind one or more mycotoxins. The different types of bentonite are each named after the respective dominant element, such as potassium (K), sodium (Na), calcium (Ca), and aluminium (Al). Bentonite usually forms from weathering of volcanic ash, most often in the presence of water. For industrial purposes, two main classes of bentonite exist: sodium and calcium bentonite. The bentonite may form between about 50% and about 75% of the binding composition.

(20) In some embodiments, any number of the components discussed herein may be combined to form a toxin binding system. By combining multiple components discussed herein into a single composition, a composition which binds mycotoxins better than the individual components may be achieved. In some embodiments, the binding composition may include organoclay, bentonite, hectorite, humic acid (e.g., Leonardite), and/or any combination thereof. As can be seen by comparing the efficacy results from the different studies detailed in the Examples section, the composition tested appears to be greater than the sum of the components forming the composition.

(21) In some embodiments, the bentonite may form between about 50% and about 75% of the binding composition. In some embodiments, the synthetic hectorite may form between about 0.5% and about 5.0% of the binding composition. In some embodiments, the organoclay may form between about 20% and about 40% of the binding composition. In some embodiments, the humic acid (e.g., Leonardite) may form between about 0.5% and about 5.0% of the binding composition.

EXAMPLES

(22) Having now described the invention, the same will be more readily understood through reference to the following example(s), which are provided by way of illustration, and are not intended to be limiting of the present invention.

Example 1

(23) In one embodiment, a toxin binding system may include an amount of an organoclay and an amount of a synthetic hectorite clay, each as described hereinabove.

(24) In some embodiments, the toxin binding system may be as follows:

(25) TABLE-US-00001 Component Amount (weight percent) Organoclay 80.0 to 99.9 Synthetic Hectorite Clay 0.1 to 20.0

Example 2

(26) In another embodiment, a toxin binding system may include an amount of a hydrated sodium calcium aluminosilicate clay and an amount of a synthetic hectorite clay, each as described herein above.

(27) In some embodiments, the toxin binding system may be as follows:

(28) TABLE-US-00002 Component Amount (weight percent) Hydrated Sodium Calcium 80.0 to 99.9 Aluminosilicate Clay Synthetic Hectorite Clay 0.1 to 20.0

Example 3

(29) In yet one other embodiment, a toxin binding system may include an amount of an organoclay, and amount of a hydrated sodium calcium aluminosilicate clay, and an amount of a synthetic hectorite clay, each as described hereinabove.

(30) In some embodiments, the toxin binding system may be as follows:

(31) TABLE-US-00003 Component Amount (weight percent) Organoclay 0.9 to 99.0 Hydrated Sodium Calcium 0.9 to 99.0 Aluminosilicate Clay Synthetic Hectorite Clay 0.1 to 20.0

Example 4

(32) Presented herein below are tests results for various components used to form one of the compositions as well as the composition for the efficacy against various toxins. TABLE I depicts the efficacy of synthetic hectorite (Laponite) against Aflatoxin, Fumonisin, Ochratoxin, and Zearalenone. TABLE II depicts the efficacy of Humic acid (Leonardite P) against Aflatoxin, Fumonisin, Ochratoxin, and Zearalenone. TABLE III depicts the efficacy of bentonite (Myco-AD D-F) against Aflatoxin, Fumonisin, Ochratoxin, and Zearalenone. TABLE IV depicts the efficacy of organoclay (Myco-AD A-Z) against Aflatoxin, Fumonisin, Ochratoxin, Zearalenone, and Endotoxin. The organoclay may be formed from 64% original bentonite (e.g., about 64%) and surfactant used to modify the original clay surface ((e.g., about 36%) (e.g., ammonium quaternary)). TABLE V depicts the efficacy of a binding composition formed from the compounds tested in TABLES I-IV combined together against Aflatoxin, Fumonisin, Ochratoxin, and Zearalenone. TABLE VI depicts the efficacy of organoclay (Myco-AD A-Z) against pesticides (e.g., several different examples of known organochlorine pesticides). The organoclay may be formed from 64% original bentonite (e.g., about 64%) and surfactant used to modify the original clay surface ((e.g., about 36%) (e.g., ammonium quaternary)). TABLE VII depicts the efficacy of Bentonite (Myco-AD D-F) against pesticides (e.g., several different examples of known organochlorine pesticides). The organoclay may be formed from bentonite. As can be seen by comparing the efficacy results from the different studies the composition tested appears to be greater than the sum of the components forming the composition.

(33) TABLE-US-00004 TABLE I SyntheticHectorite (Laponite) Aflatoxin Fumonisin Ochratoxin Zearalenone % Adsorption 92.9 82.3 32.1 46.4 94.9 86.0 33.6 49.0 95.2 88.5 32.9 52.3 % Adsorption 94.3 85.6 32.9 49.2 Average % Desorption 0.3 66.1 26.0 27.1 0.4 62.7 26.7 30.2 0.4 66.4 26.9 29.0 % Desorption 0.4 65.1 26.5 28.8 Average % Efficiency 93.9 20.5 6.4 20.4

(34) TABLE-US-00005 TABLE II Humic Acid (Leonardite P) Aflatoxin Fumonisin Ochratoxin Zearalenone % Adsorption 69.0 33.4 39.2 57.3 68.7 30.6 37.7 52.9 68.3 30.2 41.0 54.7 % Adsorption 68.7 31.4 39.3 55.0 Average % Desorption 27.3 26.7 7.8 46.5 29.2 26.1 6.9 40.2 28.8 25.9 6.8 41.8 % Desorption 28.4 26.2 7.2 42.8 Average % Efficiency 40.3 5.2 32.1 12.2

(35) TABLE-US-00006 TABLE III Bentonite (MYCO-AD D-F) Aflatoxin Fumonisin Ochratoxin Zearalenone % Adsorption 99.7 56.4 30.1 11.6 99.6 49.5 29.7 15.7 99.5 54.6 30.2 12.5 % Adsorption 99.6 53.5 30.0 13.3 Average % Desorption 0.5 42.0 27.6 9.3 0.5 39.9 26.5 10.5 0.6 43.8 28.0 10.3 % Desorption 0.5 41.9 27.4 10.0 Average % Efficiency 99.1 11.6 2.6 3.3

(36) TABLE-US-00007 TABLE IV Organoclay (MYCO-AD A-Z) Endotoxin Endotoxin Aflatoxin Fumonisin Ochratoxin Zearalenone (Run 1) (Run 2) % Adsorption 93.8 86.9 91.3 98.0 99.6 99.7 94.3 91.3 96.0 98.7 99.6 99.5 93.5 91.9 93.5 98.4 99.5 99.5 % Adsorption 93.9 90.0 93.6 98.4 99.6 99.6 Average % Desorption 13.8 6.5 0.3 1.7 0.0 0.0 11.6 6.9 0.4 1.7 0.2 0.0 15.4 6.6 0.3 1.8 0.0 0.0 % Desorption 13.6 6.7 0.3 1.7 0.1 0.0 Average % Efficiency 80.3 83.3 93.3 96.7 99.5 99.6

(37) TABLE-US-00008 TABLE V Organoclay + Bentonite + Humic Acid + Synthetic Hectorite (MYCOTOP) Aflatoxin Fumonisin Ochratoxin Zearalenone % Adsorption 99.1 97.1 97.7 99.5 99.9 98.5 98.2 99.6 99.9 97.3 97.5 99.2 % Adsorption 99.6 97.6 97.8 99.4 Average % Desorption 0.1 26.5 2.7 0.5 0.1 24.0 3.0 0.9 0.1 26.8 6.4 0.9 % Desorption 0.1 25.8 4.0 0.8 Average % Efficiency 99.5 71.8 93.8 98.6

(38) TABLE-US-00009 TABLE VI Organoclay (MYCO-AD A-Z) Heptachlor Dieldrin Diazinon Malathion (Organochlorine) (Organochlorine) (Organochlorine) (Organochlorine) % Adsorption 72.8 85.6 90.0 94.4 70.4 89.4 88.0 96.4 79.5 88.1 86.5 93.6 % Adsorption 74.2 87.7 88.2 94.8 Average % Desorption 23.8 15.1 6.5 1.0 18.9 16.1 6.4 0.7 22.4 13.1 5.6 2.1 % Desorption 21.7 14.8 6.2 1.3 Average % Efficiency 52.5 72.9 82.0 93.5

(39) TABLE-US-00010 TABLE VII Bentonite (MYCO-AD DF) Heptachlor Dieldrin Diazinon Malathion (Organochlorine) (Organochlorine) (Organochlorine) (Organochlorine) % Adsorption 90.4 85.5 32.2 27.6 91.2 86.9 34.4 24.3 91.1 85.8 35.6 29.2 % Adsorption 90.9 86.1 34.1 27.0 Average % Desorption 7.0 15.7 8.3 12.7 6.7 18.9 5.4 9.2 6.0 21.2 7.0 8.0 % Desorption 6.6 18.6 6.9 10.0 Average % Efficiency 84.3 67.5 27.2 17.0

(40) In this patent, certain U.S. patents, U.S. patent applications, and other materials (e.g., articles) have been incorporated by reference. The text of such U.S. patents, U.S. patent applications, and other materials is, however, only incorporated by reference to the extent that no conflict exists between such text and the other statements and drawings set forth herein. In the event of such conflict, then any such conflicting text in such incorporated by reference U.S. patents, U.S. patent applications, and other materials is specifically not incorporated by reference in this patent.

(41) Further modifications and alternative embodiments of various aspects of the invention will be apparent to those skilled in the art in view of this description. Accordingly, this description is to be construed as illustrative only and is for the purpose of teaching those skilled in the art the general manner of carrying out the invention. It is to be understood that the forms of the invention shown and described herein are to be taken as the presently preferred embodiments. Elements and materials may be substituted for those illustrated and described herein, parts and processes may be reversed, and certain features of the invention may be utilized independently, all as would be apparent to one skilled in the art after having the benefit of this description of the invention. Changes may be made in the elements described herein without departing from the spirit and scope of the invention as described in the following claims.